Biology-Basel最新文献_第4页

Earthworm Species from Diverse Ecological Groups Negatively Affect Enchytraeid Density in a Forest Ecosystem. 不同生态类群蚯蚓物种对森林生态系统内生虫密度的负面影响

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-17 DOI: 10.3390/biology14091283

Kamil Karaban, Anita Kaliszewicz, Krassimira Ilieva-Makulec, Alexei V Uvarov

{"title":"Earthworm Species from Diverse Ecological Groups Negatively Affect Enchytraeid Density in a Forest Ecosystem.","authors":"Kamil Karaban, Anita Kaliszewicz, Krassimira Ilieva-Makulec, Alexei V Uvarov","doi":"10.3390/biology14091283","DOIUrl":"10.3390/biology14091283","url":null,"abstract":"Earthworms and enchytraeids are two very important groups of soil organisms that influence soil biology and ecology, as well as physicochemical processes occurring in the soil. The interactions within and between these major groups of soil fauna are currently among the most pressing topics in soil ecology and are still insufficiently understood. In a field mesocosm experiment, we examined the effects of the density of the following five key earthworm species in Central Europe: Dendrobaena octaedra, Lumbricus rubellus, L. terrestris, Aporrectodea caliginosa, and Allobophora chlorotica. These species were selected to represent the following three major ecological groups of lumbricids: epigeic, endogeic, and anecic. The mesocosm experiment examined the effect of these species across density gradients and at two soil profile horizons-litter and mineral soil-and at the entire soil profile within the mesocosm. This comprehensive and unique approach was used to compare the effects of earthworm density gradient on enchytraeid abundance under identical conditions in the forest soil. The results indicate that all studied species of earthworm negatively affected enchytraeid density. The strength of this effect depended on the earthworm species, earthworm density, and the level of the analyzed soil profile. Epigeic L. rubellus, endogeic Aporrectodea caliginosa, and anecic L. terrestris appeared to be the most effective. Higher earthworm density had a greater effect on the density of enchytraeids. The most significant results concerned the litter layer, where the density of enchytraeids was highest due to their natural occurrence. Our results provide new insights into the ecological relationships among key groups of soil fauna and can be a starting point for predicting changes caused by earthworms in newly occupied ecosystems.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Inorganic Phosphorus-Solubilizing Bacteria on Rhizosphere Phosphorus Forms and Steroid Saponin Content of Paris polyphylla var. yunnanensis. 无机增磷菌对云南多叶林根际磷形态及甾体皂苷含量的影响

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-17 DOI: 10.3390/biology14091284

You Zhou, Yueheng Wang, Lingfeng Xu, Guoxin Lan, Dongqin Guo, Haizhu Zhang, Nong Zhou

{"title":"Effects of Inorganic Phosphorus-Solubilizing Bacteria on Rhizosphere Phosphorus Forms and Steroid Saponin Content of Paris polyphylla var. yunnanensis.","authors":"You Zhou, Yueheng Wang, Lingfeng Xu, Guoxin Lan, Dongqin Guo, Haizhu Zhang, Nong Zhou","doi":"10.3390/biology14091284","DOIUrl":"10.3390/biology14091284","url":null,"abstract":"To improve the phosphorus utilization efficiency and medicinal quality of the traditional Chinese medicinal herb Paris polyphylla var. yunnanensis, three inorganic phosphorus-solubilizing bacteria strains (Bacillus cereus Y1-1, Bacillus aryabhattai Z6-1, and Bacillus aryabhattai Z3-4) were inoculated individually and in combination into the rhizosphere soil of Paris polyphylla var. yunnanensis in a greenhouse pot experiment. At the late growth stage, the contents of total and inorganic phosphorus in the rhizosphere soils, as well as the total phosphorus and six steroidal saponins in the plants, were measured. The proportion of inorganic phosphorus in rhizosphere soils of treatment groups increased by 9.83~30.43%. The content of effective phosphorus form Ca2-P in the rhizosphere soils of treatment groups increased by 50.81~328.37%. Inoculation with B. aryabhattai (S2, S3, and S6) significantly increased total steroidal saponin content in plants by 9.14%, 10.64%, and 16.58%, respectively. An antagonistic effect was observed when multiple bacterial strains were inoculated together. Thus, mixed inoculation was less effective than single-strain inoculation in improving rhizosphere soil phosphorus structure. In conclusion, inoculation with inorganic phosphorus-solubilizing bacteria could enhance phosphorus availability in rhizosphere soil and improve medicinal quality of Paris polyphylla var. yunnanensis. These findings provide a basis for efficient phosphorus resource utilization and sustainable cultivation to enhance medicinal plant quality.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12468006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population Density and Spatial-Temporal Activity Pattern of the Tibetan Wolf in Dulan, Qinghai, China.

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-16 DOI: 10.3390/biology14091273

Liulin Guan, Liping Tan, Junchen Liu, Xinyang Chen, Shanshan Zhao, Guosheng Wu, Yonghong Shi, Xiao Song, Aichun Xu

{"title":"Population Density and Spatial-Temporal Activity Pattern of the Tibetan Wolf in Dulan, Qinghai, China.","authors":"Liulin Guan, Liping Tan, Junchen Liu, Xinyang Chen, Shanshan Zhao, Guosheng Wu, Yonghong Shi, Xiao Song, Aichun Xu","doi":"10.3390/biology14091273","DOIUrl":"10.3390/biology14091273","url":null,"abstract":"As apex predators, wolves (Canis lupus) play a vital role in balancing and stabilizing ecosystems. However, the scarcity of empirical data on the population size and spatial ecology of wolves in Qinghai Province, China, poses significant challenges to the design of targeted conservation measures, with cascading effects on both regional ecosystem integrity and the long-term viability of this ecologically critical species. To address this knowledge gap, we deployed a systematic grid of 150 camera traps across Dulan County, Qinghai Province, from July 2021 to July 2022. The survey effort totaled 41,610 camera-days, yielding 392 independent wolf detections, defined as events separated by ≥24 h at the same location. The random encounter model estimated wolf density across three habitat types in Dulan County as follows: alpine meadows (4.87 ± 0.87 individuals/100 km2), bare rock areas (21.39 ± 3.79 individuals/100 km2), and alpine shrub (7.81 ± 1.31 individuals/100 km2). Wolves exhibited significant habitat selection (χ2 = 23.6, p < 0.001), with 78% of detections occurring in alpine meadows and bare rocks at elevations of 4200-4300 m. The total wolf population was estimated to range from 2137 to 9169 individuals. Kernel density estimation (KDE) revealed a diurnal activity rhythm in wolves with minimal seasonal variation (Δ4 = 0.88). Peak activity intensity occurred between 8:00 and 15:00, and 17:00 and 21:00, during the cold season, and between 10:00 and 15:00, and 18:00-22:00, in the warm season. The study provides a scientific basis for more effective management and conservation, enhancing understanding of wolf populations in Qinghai and peripheral regions.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Perceived Threat Threshold from Various Drivers to Cranes Along Indus Flyway, Punjab, Pakistan. 巴基斯坦旁遮普印度河飞道不同驾驶员对起重机感知威胁阈值的比较分析

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-16 DOI: 10.3390/biology14091275

Ayesha Zulfiqar, Xueying Sun, Qingming Wu, Abdul Rehman, Nasrullah Khan, Mah Noor Khan

{"title":"Comparative Analysis of Perceived Threat Threshold from Various Drivers to Cranes Along Indus Flyway, Punjab, Pakistan.","authors":"Ayesha Zulfiqar, Xueying Sun, Qingming Wu, Abdul Rehman, Nasrullah Khan, Mah Noor Khan","doi":"10.3390/biology14091275","DOIUrl":"10.3390/biology14091275","url":null,"abstract":"Migratory birds globally face escalating anthropogenic threats, with crane species being particularly vulnerable. This study assessed human-perceived threats to cranes (Grus virgo & Grus grus) along Pakistan's vital Indus Flyway using 400 stakeholder questionnaires across eight districts (2021-2024). We quantified perceived threat based on frequency (1 = Very Rare; 5 = Very Frequent) and severity (1 = Not Severe; 5 = Extremely Severe), revealing poaching (illegal killing) as the dominant threat (frequency = 4.9; severity = 4.8), followed by illegal wildlife trade (4.7; 4.5) and taming (4.6; 4.3). Spatial analysis showed strikingly higher perceived threats in southern Pakistan (Rajanpur: frequency = 4.88, severity = 4.82) versus central regions (Khushab: 3.76, 4.02; p < 0.001), with riverbanks identified as high-risk poaching zones (42 incidents). Cluster analysis also confirmed Rajanpur as a critical hotspot within three distinct threat tiers. Critically, analysis of socio-demographic drivers revealed threat type (frequency: F = 104.92, p < 0.001; severity: F = 153.64, p < 0.001) and poaching method (frequency: F = 10.14, p < 0.001; severity: F = 15.43, p < 0.001) as significant perception-shapers, while education, occupation, and crane species preference (frequency: F = 1.17, p = 0.310) exerted a non-significant influence. These results highlight that individual backgrounds minimally modulate threat perceptions. The study aligns with global evidence of uniform crane threats demanding the following urgent conservation action: region-specific enforcement (especially southern hotspots), community-led anti-poaching initiatives, and targeted awareness programs to shift high-threat communities toward crane-friendly coexistence practices.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of Macrobenthic Communities to Heavy Metal Contamination in Sediments and Seawater: A Case Study in Temperate Bay, South Korea. 大型底栖生物群落对沉积物和海水重金属污染的响应——以韩国温带湾为例

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-16 DOI: 10.3390/biology14091276

Jian Liang, Se-Hyun Choi, Chae-Woo Ma

{"title":"Responses of Macrobenthic Communities to Heavy Metal Contamination in Sediments and Seawater: A Case Study in Temperate Bay, South Korea.","authors":"Jian Liang, Se-Hyun Choi, Chae-Woo Ma","doi":"10.3390/biology14091276","DOIUrl":"10.3390/biology14091276","url":null,"abstract":"Although effects of heavy metals in sediments on macrobenthic organisms have been extensively studied, few investigations have simultaneously assessed the influence of heavy metals in seawater and sediments on macrobenthic communities. This study investigated subtidal macrobenthic communities of Asan Bay, a coastal area in South Korea subject to extensive anthropogenic disturbances. It aimed to assess sources of heavy metals in seawater and sediments and evaluate their influence on the structure and composition of macrobenthic communities. Our findings suggest that cadmium (Cd), chromium (Cr), arsenic (As), and mercury (Hg) in seawater mainly originate from terrestrial inputs. Although the concentrations of heavy metals in seawater and sediments at most sampling stations complied with the Korean Environmental Quality Standards, the results of redundancy analysis (RDA), Spearman correlation, distance-based linear models (DistLM), and distance-based redundancy analysis (dbRDA) indicated that these heavy metals still influenced the structure of macrobenthic communities. Heavy metals in seawater were found to have a greater impact than metals in sediments, with Cr in seawater exerting the most decisive influence on macrobenthos (RDA showed that Cr explained 47.6% of the constrained species-environment relationship). Thus, Cr concentrations in bay seawaters should receive attention. Existing seawater quality standards may need to be reconsidered to reflect their effects on macrobenthic communities better.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the Transcription Factor CREB in Ethanol-Induced Endoplasmic Reticulum Stress and Apoptosis in PC12 Cells. 转录因子CREB在乙醇诱导的PC12细胞内质网应激和凋亡中的作用

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-16 DOI: 10.3390/biology14091277

Marica Németh, Barbara Brandt, Hajnalka Les, Petra Kele-Morvai, András Maifeld, Tibor A Rauch, Kristóf Schwartz, Marianna Pap

{"title":"Role of the Transcription Factor CREB in Ethanol-Induced Endoplasmic Reticulum Stress and Apoptosis in PC12 Cells.","authors":"Marica Németh, Barbara Brandt, Hajnalka Les, Petra Kele-Morvai, András Maifeld, Tibor A Rauch, Kristóf Schwartz, Marianna Pap","doi":"10.3390/biology14091277","DOIUrl":"10.3390/biology14091277","url":null,"abstract":"Ethanol is a known neurotoxic agent that induces endoplasmic reticulum (ER) stress and apoptosis in nerve cells. The transcription factor CREB is crucial for cell survival under stress; however, its involvement in ethanol-induced endoplasmic reticulum (ER) stress remains poorly understood. We examined the effects of ethanol on wild-type PC12 cells and CREB-overexpressing PC12-CREB cells. Cell viability was evaluated by ATP assays, apoptosis was detected by Hoechst staining, and key proteins involved in ER stress and apoptotic signaling were analyzed by Western blot analysis. Ethanol treatment decreased cell viability and increased apoptosis in wild-type PC12 cells in a time-dependent manner. In contrast, PC12-CREB cells-maintained viability and showed significantly lower apoptotic cell numbers. Ethanol activated markers of ER stress (BiP, CHOP, ATF6) and pro-apoptotic pathways (phosphorylation of JNK and p38 MAPK) in wild-type cells. In CREB-overexpressing cells, CHOP induction and JNK activation were decreased, while the expression of the anti-apoptotic protein Mcl-1 was increased. CREB overexpression protects against ethanol-induced ER stress and apoptosis. This protective effect is mediated through modulation of unfolded protein response (UPR) signaling and regulation of pro-and anti-apoptotic gene expression. These findings underscore a potential role for CREB in attenuating ethanol-induced neurotoxicity.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Regulation by FoxO Signaling Pathway and Muscle Remodeling Defines the Adaptive Strategy of Largemouth Bass (Micropterus salmoides) Under Saline-Alkaline Stress. FoxO信号通路与肌肉重塑的协同调控决定了大口黑鲈在盐碱胁迫下的适应策略。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-16 DOI: 10.3390/biology14091274

Guoyang Liu, Di Peng, Biyuan Liu, Qiqun Cheng

{"title":"Synergistic Regulation by FoxO Signaling Pathway and Muscle Remodeling Defines the Adaptive Strategy of Largemouth Bass (Micropterus salmoides) Under Saline-Alkaline Stress.","authors":"Guoyang Liu, Di Peng, Biyuan Liu, Qiqun Cheng","doi":"10.3390/biology14091274","DOIUrl":"10.3390/biology14091274","url":null,"abstract":"This study investigates the effects of saline, alkaline, and combined saline-alkaline water environments on the growth, muscle quality, gene expression, and metabolic profiles of largemouth bass (Micropterus salmoides). Juvenile fish were exposed to five water conditions for 60 days: freshwater (FW), saline water (SW, 10 ppt), alkaline water (AW, 15 mmol/L), and two saline-alkaline combinations (SAW-1: 4 ppt + 10 mmol/L; SAW-2: 6 ppt + 15 mmol/L). While growth rate was similar across groups, SAW-2 caused a significant decrease in survival rate and induced notable alterations in muscle texture and fiber structure. Transcriptomic analyses revealed group-specific enrichment of stress-responsive pathways. The FoxO signaling pathway acts as a central regulator of muscle maintenance and energy reallocation. The solute carrier gene slc38a4 and glula (glutamine synthetase), both closely associated with ammonia detoxification via glutamine synthesis and transport, were upregulated under saline-alkaline stress, indicating enhanced capacity for nitrogen metabolism. In addition, two key regulators of muscle remodeling, loc119898415 and tbx18, were significantly upregulated, suggesting a potential chromatin-transcription program underlying compensatory myogenesis and muscle fiber adaptation in response to environmental challenges. Metabolomic profiling showed an accumulation of osmoprotectants (betaine, taurine) in SW and SAW-2 groups, suggesting enhanced stress resistance. Multiomics integration further indicated coordinated regulation between lipid metabolism and insulin signaling, potentially mediated by the FoxO pathway. These results offer practical guidance for improving largemouth bass aquaculture under inland saline-alkaline conditions.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Antimicrobial Efficacy of Cannabinoids and Their Derivatives Against Neisseria Gonorrhoeae by Computational Analysis. 用计算分析法研究大麻素及其衍生物对淋病奈瑟菌的抑菌效果。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-15 DOI: 10.3390/biology14091272

Umairah Natasya Mohd Omeershffudin, Zakirah Zainal Abidin, Zaw Myo Hein, Che Mohd Nasril Che Mohd Nassir, Ebrahim Nangarath Kottakal Cheriya, Suresh Kumar, Muhammad Danial Che Ramli

{"title":"Investigating the Antimicrobial Efficacy of Cannabinoids and Their Derivatives Against Neisseria Gonorrhoeae by Computational Analysis.","authors":"Umairah Natasya Mohd Omeershffudin, Zakirah Zainal Abidin, Zaw Myo Hein, Che Mohd Nasril Che Mohd Nassir, Ebrahim Nangarath Kottakal Cheriya, Suresh Kumar, Muhammad Danial Che Ramli","doi":"10.3390/biology14091272","DOIUrl":"10.3390/biology14091272","url":null,"abstract":"Neisseria gonorrhoeae is a Gram-negative diplococcus that causes gonorrhea through sexual contact. This ancient STD remains a major public health concern due to reproductive health impacts, antimicrobial resistance (AMR), and lack of a vaccine. Cannabis sativa contains antibacterial cannabinoids, though its role in combating antibiotic resistance is underexplored. The 2Fe-2S iron-sulfur cluster protein is a potential antibiotic target, as these clusters are vital for bacterial proteins involved in electron transport, enzyme activity, and gene regulation. Disrupting them may impair bacterial survival and function. In this investigation, the 2Fe-2S iron sulfur cluster binding domain-containing protein (NGFG_RS03485), identified as a potential therapeutic target from the core proteome of 12 Neisseria gonorrhoeae strains, was selected for this study. Potential antimicrobial agents were explored through molecular docking studies involving 16 cannabinoid analogs-9 obtained from literature sources and 7 identified via fingerprint similarity searches. The study revealed that four cannabinoids form favorable bonds with active regions against our targeted protein; with a high binding affinity formed from the molecular docking; 1,3-Benzenediol, 2-[3-methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl]-5-pentyl-, (1R-trans). Dronabinol, Cannabinolic acid A (CBNA), Cannabigerolic acid (CBGA), and Ferruginene C are derivatives identified. Drug-likeness assessments were conducted to evaluate the pharmacokinetic and toxicity properties of the cannabinoids and compared against the antibiotics.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microglial Autophagy and Mitophagy in Ischemic Stroke: From Dual Roles to Therapeutic Modulation. 缺血性脑卒中中的小胶质细胞自噬和线粒体自噬：从双重作用到治疗调节。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-15 DOI: 10.3390/biology14091269

Juan Wu, Jiaxin Liu, Yanwen Li, Fang Du, Weijia Li, Karuppiah Thilakavathy, Jonathan Chee Woei Lim, Zhong Sun, Juqing Deng

{"title":"Microglial Autophagy and Mitophagy in Ischemic Stroke: From Dual Roles to Therapeutic Modulation.","authors":"Juan Wu, Jiaxin Liu, Yanwen Li, Fang Du, Weijia Li, Karuppiah Thilakavathy, Jonathan Chee Woei Lim, Zhong Sun, Juqing Deng","doi":"10.3390/biology14091269","DOIUrl":"10.3390/biology14091269","url":null,"abstract":"Ischemic stroke induces complex neuroinflammatory cascades, where microglial autophagy and mitophagy serve dual roles in both injury amplification and tissue repair. This scoping review synthesized current evidence on their regulatory mechanisms and therapeutic implications. Literature was identified via PubMed and Embase, yielding 79 records, from which 39 original research articles and 13 review papers were included after eligibility screening. Search terms included \"microglia,\" \"autophagy,\" and \"ischemic stroke.\" Protective autophagy was frequently associated with AMPK activation, mTOR inhibition, and mitophagy pathways such as PINK1/Parkin and BNIP3/NIX, facilitating mitochondrial clearance, M2 polarization, and anti-inflammatory signaling. Therapeutic agents such as rapamycin, Tat-Beclin 1, and Urolithin A consistently demonstrated neuroprotection in preclinical stroke models. In contrast, excessive or prolonged autophagic activation was linked to inflammasome amplification, oxidative stress, and phagoptosis. Limited human studies reported associations between elevated serum ATG5 levels or ATG7 polymorphisms and worse clinical outcomes, suggesting preliminary translational relevance. These findings support the potential of phase-specific modulation of microglial autophagy as a therapeutic avenue for stroke, although further validation in human models and development of autophagy biomarkers are needed for clinical application.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Genome Analysis of Two Bioactive Brevibacterium Strains Isolated from Marine Sponges from the Red Sea. 两株红海海绵生物活性短杆菌的综合基因组分析。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-09-15 DOI: 10.3390/biology14091271

Yehia S Mohamed, Samar M Solyman, Abdelrahman M Sedeek, Hasnaa L Kamel, Manar El Samak

{"title":"Comprehensive Genome Analysis of Two Bioactive Brevibacterium Strains Isolated from Marine Sponges from the Red Sea.","authors":"Yehia S Mohamed, Samar M Solyman, Abdelrahman M Sedeek, Hasnaa L Kamel, Manar El Samak","doi":"10.3390/biology14091271","DOIUrl":"10.3390/biology14091271","url":null,"abstract":"Marine-derived Actinomycetota have emerged as promising sources of bioactive natural products, particularly filamentous actinomycetes (e.g., Streptomyces). However, members from non-filamentous genera have showed potential biotechnological importance. In this study, we performed a comprehensive genomic characterization of two bioactive Brevibacterium strains, Brevibacterium luteolum (B. luteolum) 26C and Brevibacterium casei (B. casei) 13A, isolated from two Red Sea sponges. Whole-genome sequencing and taxonomic analysis confirmed species-level identification, marking the first documented report of these species within the Red Sea ecosystem. The two strains displayed antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans. Additionally, functional annotation revealed multiple genomic islands (GIs) enriched with genes conferring heavy metal resistance, DNA repair enzymes, nutrient acquisition, and mobile genetic elements, highlighting potential evolutionary adaptations to the harsh physicochemical conditions of the Red Sea. Genome mining identified biosynthetic gene clusters, including those encoding ε-poly-L-lysine, tropodithietic acid, ectoine, and carotenoids. The comparative analysis of orthologous gene clusters from both strains and their counterparts from terrestrial ecosystems highlighted potential marine adaptive genetic mechanisms. This study highlights the biosynthetic potential of B. luteolum 26C and B. casei 13A and their ecological role as active competitors and potential defensive associates within the sponge microbiome.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0