Biology-Basel最新文献

{"title":"Unveiling MHC-<i>DAB</i> Polymorphism Within the Western Balkan Salmonid Hotspot: Preliminary Outcomes from Native Trouts of Ohrid Lake and the Drin-Skadar Drainage (Albania).","authors":"Lorenzo Talarico, Arnold Rakaj, Lorenzo Tancioni","doi":"10.3390/biology13121060","DOIUrl":"https://doi.org/10.3390/biology13121060","url":null,"abstract":"<p><p>Due to their involvement in pathogen-mediated immune responses, the hypervariable genes of the Major Histocompatibility Complex (MHC) have become a paradigm for investigating the evolution and maintenance of genetic (adaptive) diversity, contextually providing insight into the viability of wild populations, which is meaningful for conservation. Here, we provide the first preliminary characterization of MHC polymorphism and evolution in trouts from Albania, a known hotspot of Salmonid diversity harboring ecologically and phylogenetically distinct native (threatened) taxa. Overall, 36 trout-including Lake Ohrid-endemic <i>Salmo ohridanus</i> and <i>S. letnica</i>, and both riverine and lacustrine native brown trout (the <i>S. trutta</i> complex) from the Drin-Skadar drainage-were genotyped at the MHC-<i>DAB</i> locus through next-generation amplicon sequencing. We identified 34 alleles (including 30 novel alleles), unveiling remarkable population/taxon MHC-<i>DAB</i> distinctiveness. Despite apparent functional (supertype) similarity, <i>S. letnica</i> and the <i>S. trutta</i> complex showed MHC-typical high sequence/allele diversity and evidence of global/codon-specific positive selection, particularly at antigen-binding sites. Conversely, deep-water-adapted <i>S. ohridanus</i> revealed unexpectedly reduced allelic/supertype diversity and relaxed selection. Evolution by reticulation and signals of trans-species polymorphism emerged from sequence genealogies. Further investigations and increased sampling will provide a deeper understanding of the evolutionary mechanisms yielding the observed pattern of MHC diversity across Albanian trout taxa and populations.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forces Acting on the Foot of the American Alligator (<i>Alligator mississippiensis</i>) During Pedal Anchoring.","authors":"Collin Walter, Jamie Carroll, Michael Cramberg, Jeremy J Houser, Debra Loguda-Summers, Bruce A Young","doi":"10.3390/biology13121062","DOIUrl":"https://doi.org/10.3390/biology13121062","url":null,"abstract":"<p><p>This study was undertaken to explore the forces acting on the pes during pedal anchoring and to discern if pedal anchoring required the activation of the intrinsic pedal musculature. Replica feet equipped with strain gauges were moved over mud substrate, mimicking locomotion and pedal anchoring. Quantification of the substrate tracks demonstrated that they were similar to those made by freely moving <i>Alligator</i>, that the locomotor and pedal anchoring tracks were significantly different, and that the composition of the artificial feet significantly altered the tracks. Strain gauges revealed significantly different forces at different locations (e.g., digit vs. heel) on the pes and between locomotor and pedal anchoring motions. Collectively, the results of the present study demonstrate that the forces acting on the pes during pedal anchoring are different from those during locomotion. Furthermore, varying the composition of the feet used in this study demonstrated the importance of flexion at the metatarsal/phalangeal joints. Resistance to this flexion in living crocodylians requires active muscle contraction, meaning that pedal anchoring is an active, not passive, behavior. These results offer the first insights into the mechanics of pedal anchoring and demonstrate how technologies like 3D printing can be applied to established problems like fossil trackways.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Impact of One-Year Integrated Rice-Crayfish Farming on Bacterioplankton Communities in Paddy Water.","authors":"Yiran Hou, Qiancheng Xu, Yanhong Yang, Rui Jia, Xiongjian Huang, Linjun Zhou, Bing Li, Jian Zhu","doi":"10.3390/biology13121059","DOIUrl":"https://doi.org/10.3390/biology13121059","url":null,"abstract":"<p><p>As global food security issues become increasingly severe, an important innovation in agricultural production patterns, namely integrated rice-fish farming, has been widely implemented around the world, especially in Asia. To assess the impact of integrated rice-crayfish (<i>Procambarus clarkii</i>) farming (IRCF) on agricultural ecosystems, we used Illumina high-throughput 16S rRNA gene sequencing to analyze differences in diversity, composition, co-occurrence network, and assembly process of planktonic bacterial communities in paddy water between traditional rice farming (TRM) and IRCF. Environmental factors and planktonic bacterial communities were evaluated during the tillering, jointing, flowering, and grain-filling stages on August 24, September 5, September 24, and October 16, respectively. Our findings revealed that, throughout the entire cultivation period, IRCF had no notable impacts on bacterioplankton community diversity in paddy water, but it changed the composition and relative abundance of the dominant bacterioplankton. Specifically, IRCF promoted the Chloroflexota during the tillering stage but reduced its presence during the grain-filling stage. It also significantly decreased the Bacillota during the jointing stage while notably enhancing Actinomycetota during the flowering stage. Furthermore, IRCF markedly improved the robustness and negative/positive cohesion within bacterioplankton co-occurrence networks during jointing and grain-filling stages. IRCF altered the assembly processes shaping planktonic bacterial communities, promoting a greater dominance of stochastic processes during the tillering, jointing, and flowering stages and a diminished dominance during the grain-filling stage. IRCF dramatically changed aquatic environmental factors, particularly during the jointing stage, by substantially increasing the TN, ammonium, nitrate, and phosphate levels in paddy water. These nutrient levels were closely correlated with the dynamics of the planktonic bacterial communities. Our findings underscore the considerable potential of IRCF in enhancing the stability of bacterioplankton communities and promoting rice growth while also providing valuable data and theoretical insights into the microbiological ecological impacts of IRCF on the agroecosystem.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of <i>Bacillus</i> Strains from Egyptian Mangroves: Exploring Their Endophytic Potential in Maize for Biological Control of <i>Spodoptera frugiperda</i>.","authors":"Hayam M Fathy, Mona Awad, Nawal A Alfuhaid, El-Desoky S Ibrahim, Moataz A M Moustafa, Ayatollah S El-Zayat","doi":"10.3390/biology13121057","DOIUrl":"https://doi.org/10.3390/biology13121057","url":null,"abstract":"<p><p>The widespread use of pesticides to manage <i>Spodoptera frugiperda</i> has led to significant challenges. This insect has developed resistance to 47 active insecticide ingredients. Therefore, endophytic entomopathogenic bacteria have been explored as an alternative pest management strategy, offering the potential to reduce reliance on chemical pesticides. The current study aims to evaluate the colonization potential of indigenous marine <i>Bacillus</i> strains as endophytes in maize plants and to assess their insecticidal activity against <i>S. frugiperda</i>. Four inoculation methods-foliar application, seed treatment, soil drenching, and a combination of all three-were used to establish the <i>Bacillus</i> strains as endophytes in maize plants. Our results showed that the promising native <i>Bacillus</i> strains exhibited both antibacterial and insecticidal effects against <i>S. frugiperda</i> neonates under laboratory conditions. Foliar application of <i>Bacillus</i> sp. Esh39 caused the highest mortality rate (65%), followed by <i>Bacillus tequilensis</i> R39 (60%). However, this method did not significantly enhance plant height or chlorophyll content. The potential of these native <i>Bacillus</i> strains warrants further investigation to improve biological control via endophytic mediation. Our findings provide valuable insights into the bacterial diversity and functionality of mangrove ecosystems and pave the way for innovative, sustainable insect management strategies.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Growth and Metabolic Profile of <i>Scutellaria baicalensis</i> Georgi in Response to Sodium Chloride.","authors":"Sylwester Ślusarczyk, Kajetan Grzelka, Joanna Jaśpińska, Anna Pawlikowska-Bartosz, Łukasz Pecio, Marta Stafiniak, Mehdi Rahimmalek, Wojciech Słupski, Adam Cieślak, Adam Matkowski","doi":"10.3390/biology13121058","DOIUrl":"https://doi.org/10.3390/biology13121058","url":null,"abstract":"<p><p><i>Scutellaria baicalensis</i> Georgi is a valuable medicinal plant of the <i>Lamiaceae</i> family. Its roots have been used in Traditional Chinese Medicine (under the name Huang-qin) since antiquity and are nowadays included in Chinese and European Pharmacopoeias. It is abundant in bioactive compounds which constitute up to 20% of dried root mass. These substances are lipophilic flavones with unsubstituted B-ring, baicalein, and wogonin and their respective glucuronides-baicalin and wogonoside being the most abundant. The content of these compounds is variable and the environmental factors causing this remain partially unknown. The role of these compounds in stress response is still being investigated and in our efforts to measure the effect of NaCl treatment on <i>S. baicalensis</i> growth and metabolic profile, we hope to contribute to this research. Short-term exposure to salt stress (50, 100, and 150 mM NaCl) resulted in a marked increase of baicalein from 1.55 mg to 2.55 mg/g DM (1.6-fold), baicalin from 8.2 mg to 14.7 mg (1.8-fold), wogonin from 4.9 to 6.8 (1.4-fold), and wogonoside from 3.3 to 6.8 mg/g DM (2-fold) in the roots. Conversely, in the aerial parts, the content of individual major flavonoids: carthamidine-7-O-glucuronide and scutellarein-7-O-glucuronide decreased the most by 10-50% from 18.6 mg to 11.3 mg/g (1.6-fold less) and from 6.5 mg to 3.4 mg/g DM (0.52-fold less), respectively. The amino acid profile was also altered with an increase in root concentrations of the following amino acids: arginine from 0.19 to 0.33 mg/g (1.7-fold), glutamate from 0.09 to 0.16 mg/g DM (1.6-fold), alanine from 0.009 to 0.06 mg/g (6.8-fold), proline from 0.011 to 0.029 (2.4-fold) and lysine from 0.016 to 0.063 mg/g (3.9-fold). Aspartate concentration decreased from 0.01 to 0.002 mg/g (4.8-fold less) at 150 mM NaCl. In the aerial parts, the concentration and variation in levels of specific amino acids differed among groups. For instance, the glutamate content exhibited a significant increase exclusively in the treatment group, rising from 0.031 to 0.034 mg/g, representing a 1.2-fold increase. Proline concentration showed a marked increase across all treated groups with the highest from 0.011 to 0.11 mg/g (10-fold). In conclusion, moderate salt stress was shown to increase <i>S. baicalensis</i> root biomass and flavonoid content which is rarely observed in a glycophyte species and provides a foundation for further studies on the mechanisms of osmotic stress adaptation on the specialized metabolism level.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foodborne Pathogen Prevalence and Biomarker Identification for Microbial Contamination in Mutton Meat.","authors":"Gayathri Muthusamy, Subburamu Karthikeyan, Veeranan Arun Giridhari, Ahmad R Alhimaidi, Dananjeyan Balachandar, Aiman A Ammari, Vaikuntavasan Paranidharan, Thirunavukkarasu Maruthamuthu","doi":"10.3390/biology13121054","DOIUrl":"https://doi.org/10.3390/biology13121054","url":null,"abstract":"<p><p>Microbial contamination and the prevalence of foodborne pathogens in mutton meat and during its slaughtering process were investigated through microbial source tracking and automated pathogen identification techniques. Samples from mutton meat, cutting boards, hand swabs, knives, weighing balances, and water sources were collected from four different retail sites in Coimbatore. Total plate count (TPC), yeast and mold count (YMC), coliforms, <i>E. coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Salmonella</i>, and <i>Staphylococcus</i> were examined across 91 samples. The highest microbial loads were found in the mutton-washed water, mutton meat, and cutting board samples. The automated pathogen identification system identified <i>Staphylococcus</i> species as the predominant contaminant and also revealed a 57% prevalence of <i>Salmonella</i>. Further analysis of goat meat inoculated with specific pathogens showed distinct volatile and metabolite profiles, identified using gas chromatography-mass spectrometry (GC-MS). Multivariate statistical analyses, including principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), and sparse partial least squares discriminant analysis (sPLS-DA), identified potential biomarkers for pathogen contamination. The results highlight the significance of cross-contamination in the slaughtering process and suggest the use of volatile compounds as potential biomarkers for pathogen detection.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain-Periphery Axes: The Potential Role of Extracellular Vesicles-Delivered miRNAs.","authors":"Giuseppa D'Amico, Adelaide Carista, Olga Maria Manna, Letizia Paladino, Domiziana Picone, Silvia Sarullo, Martina Sausa, Francesco Cappello, Alessandra Maria Vitale, Celeste Caruso Bavisotto","doi":"10.3390/biology13121056","DOIUrl":"https://doi.org/10.3390/biology13121056","url":null,"abstract":"<p><p>Bidirectional communication between the central nervous system (CNS) and peripheral organs and tissue has been widely documented in physiological and pathological conditions. This communication relies on the bilateral transmission of signaling molecules and substances that circulate throughout the body and reach their target site(s) via the blood and other biological fluids (e.g., the cerebrospinal fluid, the lymph). One of the mechanisms by which these molecular messengers are exchanged is through the secretion of extracellular vesicles (EVs). EVs are known to mediate cell-to-cell communication by delivering biological molecules, including nucleic acids, proteins, lipids, and various other bioactive regulators. Moreover, EVs can cross the blood-brain barrier (BBB), enabling direct communication between the periphery and the brain. In particular, the delivery of microRNAs (miRNAs) can modulate the expression profiles of recipient cells, thereby influencing their functions. This review synthesizes current findings about the brain-periphery cross-talk mediated by EVs-delivered miRNAs. Although this mechanism has been definitively shown in a few cases, much evidence indirectly indicates that it could mediate brain-peripherical organs/tissue communication, especially in pathological conditions. Therefore, understanding this process could provide valuable insights for the treatment and management of neurological and systemic diseases.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of <i>ddx4</i> Ovary-Specific Transcript Causes Dysfunction of Meiosis and Derepress of DNA Transposons in Zebrafish Ovaries.","authors":"Yuanyuan Chen, Xing Lin, Jing Dai, Yifan Bai, Fei Liu, Daji Luo","doi":"10.3390/biology13121055","DOIUrl":"https://doi.org/10.3390/biology13121055","url":null,"abstract":"<p><p>Alternative splicing of <i>ddx4</i> (DEAD-box helicase 4), a key germline marker gene, has been reported to generate sex-specific transcripts in zebrafish gonads. The biological functions and regulatory mechanisms of the <i>ddx4</i> ovary-specific transcript (<i>ddx4-L</i>) during oogenesis remain unclear. In this study, we found that <i>ddx4-L</i> mutants, in which <i>ddx4-L</i> was specifically deleted, had enlarged ovaries but laid fewer eggs, along with having a lower fertilization rate compared to WT controls. RNA-seq analysis was performed to detect the changes in gene expression between WT and <i>ddx4-L</i> mutant ovaries. A total of 524 upregulated and 610 downregulated DEGs were identified. GO and GSEA enrichment analyses showed that genes involved in fertilization and reproduction biological processes were significantly downregulated. More specifically, we observed a remarkable reduction in Sycp1, a core component of synaptonemal complex, in <i>ddx4-L</i> mutant ovaries at both the mRNA and protein levels. In addition, the expressions of transposon elements, as well as the events of alternative splicing, alternative polyadenylation, and RNA editing, were analyzed based on the RNA-seq data. We found that the deletion of <i>ddx4-L</i> resulted in derepression of DNA transposons in zebrafish ovaries, possibly causing genome instability. In conclusion, our work demonstrates that the ovary-specific <i>ddx4</i> transcript plays important roles in oocyte meiosis and DNA transposon repression, which extends our understanding of the biological functions and regulatory mechanisms of sex-specific alternative splicing in zebrafish oogenesis and reproduction.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolating and Identifying One Strain with Lead-Tolerant Fungus and Preliminary Study on Its Capability of Biosorption to Pb².","authors":"Wanna Li, Liujing Zhao, Cui Liu, Xingpeng Bai, Chenyuan Xu, Fengqiu An, Feilong Sun","doi":"10.3390/biology13121053","DOIUrl":"https://doi.org/10.3390/biology13121053","url":null,"abstract":"<p><p>To obtain lead-resistant microorganisms as potential strains for bioremediation, in this study, a strain of fungus with high resistance to lead was isolated and domesticated from lead-contaminated soil, which was cultured and molecularly biologically identified as the genus Sarocladium Pb-9 (GenBank No. MK372219). The optimal incubation time of strain Pb-9 was 96 h, the optimal incubation temperature was 25 °C, and the optimal incubation pH was 7. The strain Pb-9 had a good adsorption effect on Pb²⁺ at a lead concentration of 2000 mg/L; scanning electron microscopy (SEM) observed that the spores of the Pb-9 strain appeared to be wrinkled and deformed under Pb²⁺ stress, and XRD analysis showed that the mycelium of Pb-9 adsorbed Pb²⁺; Fourier transform infrared spectroscopy (FTIR) analysis showed that the Pb-9 strain might produce substances such as esters and polysaccharides under the treatment of different Pb²⁺ concentrations. The above results showed that strain Pb-9 has good resistance and adsorption capacity to lead. Therefore, it has potential application value in the bioremediation of environmental heavy metal pollution, and this study provides a fundamental basis for the bioremediation of lead pollution in the environment.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adenoviral Vectors for Gene Therapy of Hereditary Diseases.","authors":"Anna Muravyeva, Svetlana Smirnikhina","doi":"10.3390/biology13121052","DOIUrl":"https://doi.org/10.3390/biology13121052","url":null,"abstract":"<p><p>Adenoviral vectors (AdVs) are effective vectors for gene therapy due to their broad tropism, high capacity, and high transduction efficiency, which makes them actively used as oncolytic vectors and for creating vector vaccines. However, despite their numerous advantages, AdVs have not yet found their place in gene therapy for hereditary diseases. This review provides an overview of AdVs, their features, and clinical trials using them for gene replacement therapy in monogenic diseases and analyzes the reasons for the failures of these studies. Additionally, current research on the modification of AdVs to reduce immune responses and target delivery is discussed.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"13 12","pages":""},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11673936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}