Biology-Basel最新文献

{"title":"Genetic Relationships, Whole-Fruit Oil Variations, and Quantitative Trait Diversity in Elite <i>Symplocos paniculata</i> Accessions from Hunan Revealed by GBS.","authors":"Jiating Zhao, Beilei Xie, Lijuan Jiang, Yan Yang, Changzhu Li, Jingzhen Chen, Peiwang Li, Yanju Song, Xiao Zhou, Qiang Liu, Yunzhu Chen","doi":"10.3390/biology15191702","DOIUrl":"10.3390/biology15191702","url":null,"abstract":"<p><p><i>Symplocos paniculata</i> is a promising woody oil species, but the genetic diversity and joint variation in whole-fruit, tissue-specific oil, fatty acid, and quantitative traits remain insufficiently characterized. Thirty elite accessions from six geographic origins in Hunan Province were evaluated using genotyping-by-sequencing, whole-fruit and tissue-specific oil measurements, fatty acid profiling, and 12 quantitative traits. A total of 6335 single-nucleotide polymorphisms revealed geographic differentiation together with substantial within-origin heterogeneity. The principal diversity, PCoA, AMOVA, and pairwise FST patterns remained stable after exclusion of a high-missingness accession. Whole-fruit oil content averaged 34.91% and ranged from 18.54% to 51.35%, with oleic acid as the predominant fatty acid. An additional tissue-specific experiment showed higher mean oil contents in non-seed than seed tissues (37.22% vs. 30.32%) and clear differences in fatty acid composition. Dry-mass-weighted reconstruction closely reproduced measured whole-fruit oil content (Pearson r = 0.935), indicating that whole-fruit variation reflects tissue-specific oil accumulation rather than tissue proportion alone. In the original 72-test analysis, pooled morphology-oil associations did not retain Benjamini-Hochberg significance after adjustment for geographic origin or genetic structure. In a separate 15-test tissue-specific reassessment, infructescence width remained positively associated with non-seed oil content in pooled, origin-adjusted, and genetic-adjusted models. These results characterize complementary genetic and oil-related variations among the sampled accessions and identify tissue-specific relationships that warrant independent validation in replicated common-garden and multi-year trials.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of Rocket Fuel Derivatives to the <i>Tenebrio molitor</i>-<i>Gregarina</i> spp. Host-Parasite System at Different Temperatures.","authors":"Denis Rybalka, Viktor Brygadyrenko","doi":"10.3390/biology15191700","DOIUrl":"10.3390/biology15191700","url":null,"abstract":"<p><p>Hydrazine (N₂H₄) is a component of rocket fuel, and its dinitroaromatic relatives are feedstocks for explosives and pesticides. Both classes contaminate soils around aerospace facilities and in areas of armed conflict. The toxicity of hydrazine hydrate, 2,4-dinitrophenylhydrazine (DNPH) and 2,4-dinitroaniline to terrestrial invertebrates remains unexplored. This study examined the effects of these substances on the mortality, body mass, pupation, and intensity of <i>Gregarina</i> spp. infestation in larvae of <i>Tenebrio molitor</i> Linnaeus, 1758 (Coleoptera, Tenebrionidae). Mortality rose with dose for all three compounds and at 90 mg/kg reached 26.7%. Pupation responded to the fact of exposure itself: none was recorded in the control (0.0%), whereas 59.6% of the exposed larvae pupated, already at the lowest concentration of 10 mg/kg, where no deaths occurred. The proportion pupating was similar across the three compounds (55.0-62.2%) but declined with increasing concentration, from 67.2% at 10 mg/kg to 52.2% at 90 mg/kg. Within the 21-28 °C range, no main effect of temperature on mortality, pupation, or body mass was found. All three compounds reduced the intensity of <i>Gregarina</i> spp. infestation with increasing concentration. Under acute 5-day exposure, therefore, the studied compounds cause little larval mortality but alter pupation, body mass and the intensity of parasitic infestation.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, Physiological Time and Reproductive Potential of <i>Bradysia impatiens</i> (Diptera: Sciaridae) from Liuba, Shaanxi, with a Review Pertaining to Its Occurrences in China.","authors":"Qi Wang, Limin Zhao, Ya Sun","doi":"10.3390/biology15191696","DOIUrl":"10.3390/biology15191696","url":null,"abstract":"<p><p>The sciarid fly <i>Bradysia impatiens</i> (Johannsen) is one of the key pests on American ginseng <i>Panax quinquefolium</i> L. in Liuba County, Shaanxi Province, China. Its living specimens were sampled, isolated, reared, mounted, observed and microphotographed. Its identification was completed through both comparisons of morphotaxonomic characteristics and DNA barcoding, and its occurrences in China were reviewed. After conducting serial rearing experiments of the insect at eight temperatures, we calculated developmental threshold temperatures (<i>C</i>) and thermal constants (<i>K</i>) of the species in its egg, larval, pupal and generational stages, and then constructed four reciprocal models <i>N</i> = <i>K</i>/(<i>T</i> - <i>C</i>), where <i>N</i> denotes developmental duration and <i>T</i> is rearing temperature. With values of <i>K</i> and <i>C</i> as well as their <i>SE</i> written, the reciprocal model for its generational stage is <i>N_generation</i> = (374.9 ± 8.15)/[<i>T</i> - (6.6 ± 0.29)]. Based on <i>C</i> and <i>K</i> and annual effective accumulated temperatures, we estimated that the sciarid pest would reproduce at a rate of 4.2 to 5.7 generations a year in local regions. In the laboratory, the fecundity of the species was tested to be 59.2 eggs per female, the survival rate of the immature individuals averaged 46.4% in the range from 10.0 to 30.0 °C, and the sex ratio of female to male was 2:1. Based on these coefficients, we are able to estimate the reproductive potential that a parent female and her offspring may reach in a year if they live under favorable conditions, and corresponding economic losses.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inter- and Intraspecific Variation in Dorsal Pigmentation Patterns of Skates (Rajidae) from the Cantabrian Sea: Evidence of High Variability in <i>Raja clavata</i> and Implications for Species Identification.","authors":"Clara Piedrafita-Zubillaga, Daniel A Gómez-Lobo, Isabel Gómez-Álvarez, Johanne Wauters, Pablo de la Calva-Castiñeira, Trinidad Pérez, Ángel Muñoz, Yaisel J Borrell, Laura Miralles","doi":"10.3390/biology15191698","DOIUrl":"10.3390/biology15191698","url":null,"abstract":"<p><p>Accurate species identification is essential to assess population trends and implement effective conservation measures. Many rajid species have life-history characteristics that increase their vulnerability to fishing mortality, while their conservation status differs among species. However, species identification within the family Rajidae is hampered by morphological conservatism and intraspecific phenotypic variation, which frequently lead to misidentification in fisheries surveys and seafood markets. Here, we combine COI DNA barcoding with a detailed photographic assessment of dorsal pigmentation patterns in 484 skate specimens collected from a fish wholesaler market in the Cantabrian Sea. Seven species were genetically identified as <i>Raja clavata</i>, <i>Raja montagui</i>, <i>Raja microocellata</i>, <i>Raja undulata</i>, <i>Raja brachyura</i>, <i>Leucoraja naevus</i>, and <i>Leucoraja circularis.</i> Substantial overlap in pigmentation patterns was detected among genetically distinct species, while <i>R. clavata</i> exhibited nine distinct morphotypes, five of which have not been previously described. We further investigated whether <i>R. maderensis</i> individuals display dorsal patterns distinct from those of <i>R. clavata</i>. Notably, a dark-crossbar pattern, previously unreported in <i>R. montagui</i>, was recorded in this species. Moreover, an unusually extreme case of hypomelanosis in <i>R. microocellata</i> is also discussed. Together, these findings demonstrate that dorsal pigmentation alone in processed individuals is insufficient for reliable species discrimination in skates and highlights the need to integrate molecular tools into fisheries monitoring and biodiversity assessments.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Basal Sources to Top Predators: Isotopic Evidence of Trophic Organization in the Francisco Coloane Coastal Marine Protected Area.","authors":"Lisette Zenteno-Devaud, Gabriela V Aguirre-Martínez, Matías Cárcamo-Chávez, Paola Acuña-Gómez, Jorge Acevedo, Claudio A Moraga, Lautaro Oyarzún-Galaz, Pedro Valenzuela, Anelio Aguayo-Lobo","doi":"10.3390/biology15191699","DOIUrl":"10.3390/biology15191699","url":null,"abstract":"<p><p>Coastal food webs are sustained by two contrasting energy channels-a fast pelagic pathway fueled by phytoplankton and a slow benthic pathway built on detrital processing-yet whether these channels remain segregated or become progressively coupled as energy moves toward higher consumers is still poorly resolved, particularly in remote, environmentally variable systems. We tested these competing scenarios-channel compartmentalization versus channel coupling-using carbon (δ¹³C) and nitrogen (δ¹⁵N) stable isotopes across a four-level food web in the Francisco Coloane Marine Coastal Protected Area, a subantarctic system in the Strait of Magellan, Chile. Lower-trophic consumers showed strong isotopic differentiation, tracking access to distinct basal resources, but this segregation gave way to substantial niche convergence at intermediate trophic levels, where overlap probabilities exceeded 60%. Macroalgal-derived carbon dominated assimilation across resident consumers (50-80%), while sediment organic matter acted as an integrative pool rather than an independent pathway, blending detrital inputs from multiple sources. The clearest evidence for channel coupling came from the squat lobster <i>Grimothea gregaria</i>: its pelagic and benthic life stages occupied opposite ends of the isotopic gradient, showing that a single species can physically cross channels that appear isotopically segregated at the community level. Together, these results indicate that benthic and pelagic energy pathways in this protected area are not independent but progressively integrated as energy moves up the food web-a pattern with direct implications for how spatial management should treat habitat connectivity in coastal marine reserves.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated lncRNA and mRNA Transcriptome Analysis Reveals Candidate Regulatory Networks Associated with Postnatal <i>Longissimus Dorsi</i> Muscle Development in Hanper Sheep.","authors":"Xiaoyong Chen, Zhen Wang, Rui Yuan, Ying Bai, Lei Liu, Guanghui Li","doi":"10.3390/biology15191694","DOIUrl":"10.3390/biology15191694","url":null,"abstract":"<p><p>Skeletal muscle development is a key determinant of meat production and meat-quality traits in sheep, but the coordinated long non-coding RNA (lncRNA) and messenger RNA (mRNA) co-expression landscape underlying postnatal muscle development in Hanper sheep remains unclear. In this study, RNA sequencing was performed on <i>Longissimus Dorsi</i> muscle samples from Hanper sheep at 1 month (M1), 7 months (M7), and 13 months (M13) of age to characterize lncRNA and mRNA expression profiles during postnatal growth. A total of 13,940 candidate lncRNAs were identified, and these lncRNAs exhibited typical structural features, including shorter transcript lengths, fewer exons, and shorter open reading frames than mRNAs. Differential expression analysis identified 39, 11, and 174 differentially expressed lncRNAs in M7_vs_M1, M13_vs_M7, and M13_vs_M1, respectively, while 357, 160, and 446 differentially expressed mRNAs were detected in the corresponding comparisons. Functional enrichment analyses showed that differentially expressed mRNAs and co-expressed mRNAs associated with differentially expressed lncRNAs were mainly involved in RNA metabolism, translation, ribosome biogenesis, amino acid biosynthesis, energy and lipid metabolism, extracellular matrix remodeling, and cell adhesion. Several muscle-development-related signaling pathways, including AMPK, insulin, PI3K-Akt, PPAR, mTOR, FoxO, ECM-receptor interaction, and focal adhesion pathways, were also enriched. Integrated lncRNA-mRNA analysis further identified candidate lncRNA-associated differentially expressed genes, including <i>IGF2</i>, <i>FOS</i>, <i>ACTC1</i>, <i>ADIPOQ</i>, <i>SPP1</i>, <i>PECAM1</i>, and <i>SLC16A7</i>. These findings provide a comprehensive lncRNA and mRNA transcriptomic resource and reveal candidate regulatory molecules potentially involved in postnatal <i>Longissimus Dorsi</i> muscle development in Hanper sheep.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Genomics of <i>NGF</i>, <i>NTRK1</i>, and <i>NGFR</i> Across Chordates: Joint Gene Recovery and Region-Specific Constraint.","authors":"Zhimin Li, Xiaojie Liu, Ya Zhang, Yeping Tan","doi":"10.3390/biology15191695","DOIUrl":"10.3390/biology15191695","url":null,"abstract":"<p><p>Nerve growth factor (NGF) is a neurotrophin central to sensory neuron biology and pain signaling. <i>NGF</i> and <i>NTRK1</i> are gnathostome paralogs within the older neurotrophin and Trk families, respectively, whereas <i>NGFR</i> encodes p75NTR, a member of the tumor necrosis factor receptor superfamily. Orthology assessment of <i>NGF</i>, <i>NTRK1</i>, and <i>NGFR</i> across vertebrates is challenging because mature neurotrophins are short, cysteine-rich ligands, <i>NTRK1</i> has closely related paralogs, and <i>NGFR</i> lies outside the Trk receptor family. We assessed species-level joint genomic recovery of the three genes and compared evolutionary constraint among predefined ligand and receptor regions. The analysis covered 94 chordates and combined graded recovery evidence with family phylogenies, locus and domain evidence, codon models, amino acid conservation, and structural mapping. Sixteen non-gnathostome species were included to provide evolutionary context and were not used to calculate recovery rates for the three focal genes. Among 78 sampled gnathostomes, 52 had high-confidence joint recovery, eight had supported provisional joint recovery, four had component-level evidence consistent with possible co-recovery, and 14 remained incomplete. Mature NGF had a numerically lower estimate of the ratio of nonsynonymous to synonymous substitution rates (dN/dS, ω) than the pro-domain (0.067 versus 0.352). All six scaffold cysteines were invariant; in the residue-class comparison, the cysteine scaffold was the only structural class that differed from the coordinate-resolved background after correction. The dimer-interface, TrkA-contact, and p75NTR-contact classes did not differ significantly from that background after correction. Receptor analyses yielded low ω estimates in Trk kinase domains and p75NTR intracellular regions. Together, these results provide a confidence-graded map of focal-gene recovery and regional purifying constraint while placing non-gnathostome homologs within the older neurotrophin system.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Seedling Grade on Biomass Accumulation and Allometric Growth Across Developmental Stages in <i>Pinus yunnanensis</i> Franch. Seedlings.","authors":"Pengrui Wang, Honglin Wang, Zhuangyue Lu, Xiangyang Kang, Yulan Xu, Nianhui Cai, Boning Yang","doi":"10.3390/biology15191692","DOIUrl":"10.3390/biology15191692","url":null,"abstract":"<p><p><i>Pinus yunnanensis</i> Franch. holds significant ecological value as a native conifer endemic to the southwestern uplands of China. The annual <i>P. yunnanensis</i> seedlings were categorized into three quality classes: Grade I, Grade II, and Grade III in accordance with the national specifications for seedling quality. We measured root, stem, needle, aboveground, and individual biomass at six stages from 240 to 690 days. We used the standardized major axis method to analyze allometric relationships among organs and with total biomass. Our results showed that Grade I seedlings had more stable allometric coordination over time. The lower grades showed more fluctuations and weaker coordination. Needles dominated biomass throughout the development period, roots grew faster in later stages, and stems grew slower but increased their share. This showed seedlings invest first in needles for carbon gain and then gradually build support and uptake structures. The biomass allocation and growth modes matured between 510 and 690 days. Biomass differed among seedling grades. Grade I seedlings show better growth stability and resource-use efficiency. They are suitable for nursery cultivation and afforestation. This study lays the groundwork for upgrading quality standards and perfecting silvicultural techniques. It also helps design precise tending measures to raise survival rates and early productivity. This work has practical value for plantation establishment, ecological restoration, and timber development in Southwest China.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population Genomic Assessment of Genetic Diversity and Structure in Mackerel Scad (<i>Decapterus macarellus</i>) in the South China Sea Based on RAD-seq.","authors":"Yi Li, Shuai Zhang, Min Li, Jiangtao Fan, Yancong Cai, Yutao Yang, Shannan Xu, Zuozhi Chen","doi":"10.3390/biology15191697","DOIUrl":"10.3390/biology15191697","url":null,"abstract":"<p><p>The mackerel scad (<i>Decapterus macarellus</i>) is an important pelagic fish in the South China Sea (SCS), where it supports light purse-seine fisheries. Genome-wide information on its genetic diversity and population structure remains limited, although it is needed for stock identification and sustainable management. Restriction-site-associated DNA sequencing (RAD-seq) was used to analyze 112 <i>D. macarellus</i> individuals collected from Xisha, Dongsha, and Nansha Islands in the SCS, with Dongsha and Nansha sampled in April 2025 and Xisha sampled in September 2025; the congener <i>Decapterus macrosoma</i> was included as an outgroup for analyses requiring an external reference. A total of 12,080 high-quality single-nucleotide polymorphisms (SNPs) across 1087 retained loci were used for within-species analyses. The three sampling groups showed moderate-to-high genetic diversity (π = 0.02045-0.03191; <i>H</i>_O = 0.01669-0.02497). Pairwise F_ST values ranged from 0.0086 to 0.0120 and D_XY values ranged from 0.00156 to 0.00174, while principal component analysis, ADMIXTURE, and phylogenetic analyses indicated low genome-wide differentiation among the sampled regions. The TreeMix results were treated as exploratory and showed close allele-frequency relationships among the sampling groups. Genotype-environment association analysis identified putative association signals across the environmental variables examined, with chlorophyll-a, salinity, and nitrate yielding the largest numbers of candidate loci. Overall, these results indicate low neutral genetic differentiation among the sampled groups. Because sampling location and month were confounded and the data are based primarily on neutral genomic markers, the present results should be considered a genomic baseline rather than definitive evidence for a single fishery management unit.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered IRE1α-Dependent Decidual Programming Links Impaired Inflammation and Angiogenesis in Recurrent Implantation Failure.","authors":"Lara Castagnola, Lourdes Materazzi, Daniel Paparini, Ana Schafir, Marcela Irigoyen, Esteban Grasso, Nancy Charó, Daiana Vota, Agustina Marcial, Diego Gnocchi, Alejandro Gustavo Martínez, Antonio Cattaneo, Lautaro Tessari, Vanesa Hauk, Soledad Gori, Rosanna Ramhorst","doi":"10.3390/biology15191693","DOIUrl":"10.3390/biology15191693","url":null,"abstract":"<p><strong>Background: </strong>Decidualization alters the endometrial stromal cells (EnSC) secretome, allowing sterile inflammation associated with embryo implantation, but mechanisms that lead to alterations in this process are still unknown. We evaluated whether impaired IRE1α-mediated endoplasmic reticulum (ER) stress signalling contributes to defective angiogenic decidual programming in patients with recurrent implantation failure (RIF).</p><p><strong>Methods: </strong>Candidate pathways were identified with bioinformatic analyses of endometrial arrays and validated in endometrial biopsies from fertile and RIF women (secretory phase) by RT-qPCR and in isolated EnSC by flow cytometry. Functional alterations were evaluated using trophoblast spheroid and endothelial cell migration assays. ER stress was pharmacologically induced in EnSC using thapsigargin.</p><p><strong>Results: </strong>RIF endometria displayed impaired in vivo decidualization, with reduced expression of <i>IGFBP1</i> and <i>PGR</i>, together with altered expression of molecules involved in blastocyst adhesion. Conditioned media from RIF-derived EnSC-impaired trophoblast outgrowth. RIF samples displayed an anti-angiogenic and altered inflammatory profile, characterized by reduced intracellular IL-1β accumulation, decreased <i>VEGFA</i>, and increased <i>sFlt1</i> expression. Bioinformatic analyses identified IRE1α pathway as a central altered network associated with angiogenesis. Consistently, RIF samples showed reduced expression of <i>IRE1α</i>, <i>sXBP1</i>, and <i>NLRP3</i>. Moreover, ER stress induction in EnSC promoted endothelial cell migration.</p><p><strong>Conclusions: </strong>RIF is associated with defective angiogenic decidual programming linked to impaired IRE1α-mediated ER stress signalling. These findings identify the IRE1α axis as a potential pathway of interest for future therapeutic exploration in recurrent implantation failure.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}