Biology-Basel最新文献

{"title":"Simulating Habitat Suitability Changes of Threadfin Porgy (<i>Evynnis cardinalis</i>) in the Northern South China Sea Using Ensemble Models Under Medium-to-Long-Term Future Climate Scenarios.","authors":"Junyi Zhang, Jiajun Li, Yancong Cai, Kui Zhang, Youwei Xu, Zuozhi Chen, Shannan Xu","doi":"10.3390/biology14030236","DOIUrl":"10.3390/biology14030236","url":null,"abstract":"<p><p>The impact of global warming on fish distribution is a key factor in fishery management and sustainable development. However, limited knowledge exists regarding the influence of environmental factors on the distribution of <i>Evynnis cardinalis</i> under climate change. This study addresses this gap by predicting the species distribution under current conditions and three future climate scenarios (SSP126, SSP370, and SSP585) using five individual models and four ensemble models. The results demonstrate that the ensemble models outperform the single models, with majority voting (EMca) achieving the highest accuracy (ROC = 0.97, TSS = 0.85). Bathymetry (BM) and the sea surface height (SSH) are the primary factors influencing the distribution. The predictions indicate that the currently suitable habitats of <i>E. cardinalis</i> are primarily located in the Beibu Gulf region of the northern South China Sea. Under future climate scenarios, suitable habitat areas are expected to expand to higher latitudes and deeper waters, though highly suitable habitats in the western Guangdong coastal waters, western Beibu Gulf, and southwestern offshore waters of Hainan Island will significantly decrease.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Prenatal Herbal Methionine Supplementation on Growth Indices, Onset of Puberty, Blood Metabolites, and Fertility of Alpine Doelings.","authors":"Venancio Cuevas-Reyes, Jorge Flores-Sánchez, Esau Ramírez de la Cruz, Juan M Vázquez-García, Luisa E S Hernández-Arteaga, Reagan Sims, Jaime M Cavazos-Galindo, Miguel Mellado, César A Rosales-Nieto","doi":"10.3390/biology14030237","DOIUrl":"10.3390/biology14030237","url":null,"abstract":"<p><p>This study investigated the effects of prenatal herbal methionine supplementation on growth, puberty onset, reproductive efficiency, and blood metabolites in first-generation G1 Alpine doelings. Sixty pregnant multiparous goats (G0), each with at least three parturitions, were assigned during the last trimester of pregnancy to either herbal methionine (H-MET-G0; 1% Optimethione^®; n = 30) or a control group (CTL-G0; n = 30). Only female offspring (G1 doelings) were studied (H-MET-G1:19; CTL-G1:25) from weaning (45 days old) to 10 months (first breeding). G1 doelings were weighed and monitored weekly for metabolites, IGF-1, insulin, and progesterone. Ovulation of the G1 doelings was induced using vasectomized bucks, followed by breeding with intact bucks. Postweaning growth, age at puberty (H-MET-G1:216 vs. CTL-G1:229 days), and live weight at puberty (H-MET-G1:20.5 vs. CTL-G1:21.0 kg) did not significantly differ (<i>p</i> > 0.05). Age at puberty and conception were negatively correlated with live weight (<i>p</i> < 0.001). First-cycle conception rates were 81% in CTL-G1 and 66% in H-MET-G1, with similar days to conception (<i>p</i> > 0.05). The number of pregnant doelings and the number of kids in utero did not significantly differ (<i>p</i> > 0.05) between treatments. Plasma IGF-1 and insulin levels were significantly higher (<i>p</i> < 0.001) in H-MET-G1 doelings, whereas plasma metabolites related to nutrition showed no differences between groups (<i>p</i> > 0.05). Prenatal herbal methionine supplementation had no carry-over effect on growth, puberty onset, or reproductive efficiency. Age at puberty and conception were negatively correlated with live weight and positively correlated with IGF-1 and insulin levels.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Climate-Driven Mismatches Between Pollinator-Dependent Crops and Honeybees in Asia.","authors":"Ehsan Rahimi, Chuleui Jung","doi":"10.3390/biology14030234","DOIUrl":"10.3390/biology14030234","url":null,"abstract":"<p><p>In Asia, <i>Apis cerana</i> (native) and <i>Apis mellifera</i> (introduced) are the primary managed honeybee species, vital for pollination and honey production. However, climate change and other threats are driving pollinator declines, while research on their ecology in Asia remains limited. Bridging these knowledge gaps is crucial for developing conservation strategies to sustain pollination services and agricultural systems in the region. In this study, we evaluated the potential impacts of climate change on the spatial interactions between two honeybee species (<i>A. cerana</i> and <i>A. mellifera</i>) and 20 pollinator-dependent crops across 23 countries in Asia. We used species distribution models (SDMs) to generate habitat suitability maps for both honeybees and crops under current and future climate scenarios (SSP585 for 2070). Schoener's D statistic was employed to quantify the spatial overlap, and a novel spatial approach was applied to create mismatch maps that identified areas of increased or decreased interactions. We found that, on average, <i>A. cerana</i> demonstrated higher overlap with 12 crops compared to 8 for <i>A. mellifera</i> in future projections. Key crops like sesame, eggplant, and mango retained strong overlaps with both pollinators, while mismatches were more pronounced for <i>A. cerana</i>, particularly with watermelon, strawberry, and buckwheat. In contrast, <i>A. mellifera</i> showed greater stability and resilience in spatial overlaps with crops such as soybean and sunflower. Overall, <i>A. cerana</i> is expected to face more significant spatial mismatches, highlighting its vulnerability to climate-induced changes and the critical need for targeted conservation efforts. This study highlights the urgent need for region-specific conservation strategies by identifying areas at risk of losing critical pollination services. Our results provide a crucial framework for informed management and policy interventions to safeguard pollination-dependent agricultural systems and biodiversity in Asia amidst accelerating environmental challenges.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light Exposure, Physical Activity, and Indigeneity Modulate Seasonal Variation in NR1D1 (REV-ERBα) Expression.","authors":"Denis Gubin, Sergey Kolomeichuk, Konstantin Danilenko, Oliver Stefani, Alexander Markov, Ivan Petrov, Kirill Voronin, Marina Mezhakova, Mikhail Borisenkov, Aislu Shigabaeva, Julia Boldyreva, Julianna Petrova, Dietmar Weinert, Germaine Cornelissen","doi":"10.3390/biology14030231","DOIUrl":"10.3390/biology14030231","url":null,"abstract":"<p><p>Nuclear receptor subfamily 1 group D member 1 (NR1D1 or REV-ERBα) is a crucial element of the circadian clock's transcriptional and translational feedback loop. Understanding its expression in humans is critical for elucidating its role in circadian rhythms and metabolic processes, and in finding potential links to various pathologies. In a longitudinal survey, we examined REV-ERBα expression at 08:00 using a real-time polymerase chain reaction (qRT-PCR) in blood mononuclear cells from Arctic native and non-native residents during equinoxes and solstices. REV-ERBα expression exhibited a pronounced seasonality, peaking at the summer solstice, and reaching a nadir at the winter solstice in both natives and non-natives, with a relatively higher summer peak in natives. After adjusting for age, sex, and body mass index, the amount and timing of light exposure, the amount of physical activity, and indigeneity emerged as significant predictors of REV-ERBα expression.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of Root Rot (<i>Rhizoctonia solani</i> Kühn) of Common Bean Using Host Resistance and Consortia of Chemicals and Biocontrol Agents.","authors":"Pratikshya Parajuli, Ritesh Kumar Yadav, Hira Kaji Manandhar, Megha N Parajulee","doi":"10.3390/biology14030235","DOIUrl":"10.3390/biology14030235","url":null,"abstract":"<p><p>Rhizoctonia root rot and web blight caused by <i>Rhizoctonia solani</i> Kühn is one of the destructive diseases of the common bean (<i>Phaseolus vulgaris</i> L.). A two-year study was conducted (2019-2021) to evaluate the common bean landraces and genotype, biocontrol agents, and chemical fungicides against the pathogen. Nine landraces from high-altitude agriculture in the Jumla district in western Nepal (29.27443900, 82.19332200; 2540 m msl) and one genotype of commercial common bean from the sub-tropical Chitwan district in central Nepal (27.65186564, 84.34869288; 256 m masl) were evaluated for resistance against the pathogen under screenhouse and field conditions. All were found highly susceptible to the pathogen and the highest lesion length (3.02 cm) was measured in the <i>Rato Male Simi</i> followed by <i>Rato Sano Chhirke Simi</i> (2.40 cm) in Chitwan conditions. Three chemical fungicides-SAAF™ (carbendazim 12% + mancozeb 63% WP), Vitavax^® (carboxin 37.5% + thiram 37.5% DS), and Nativo^® (tebuconazole 50% + trifloxystrobin 25% <i>w</i>/<i>w</i> (75 WG)-and three biocontrol agents-<i>Trichoderma viride</i> (10⁹ cfu/mL), <i>Pseudomonas fluorescens</i> (10⁹ cfu/mL), and <i>Bacillus subtilis</i> (10⁸ cfu/mL)-were evaluated as seed and soil treatments in fourteen different combinations against <i>R. solani</i>. SAAF™ and Vitavax^® were found highly effective as they completely suppressed the incidence of pre-emergence damping off. SAAF™ was equally effective followed by Vitavax^® and Nativo^® in suppressing post-emergence damping off. On the other hand, the highest disease incidence (26.63%) was recorded in treatment where seed treatment with <i>Pseudomonas fluorescens</i> formulation at the rate of 10 g per kg of FYM slurry along with soil application with <i>Pseudomonas fluorescens</i> formulation at the rate of 10 kg per ha with 50 kg FYM was applied, whereas the lowest disease incidence (4.96%) occurred in SAAF™ for both seed and soil applications. Highest grain yield (4125 kg/ha) was recorded on Nativo^® where seed treatment at the rate of 1.5 g per kg along with soil drenching at the rate of 1.5 g per liter was used. Thus, it can be concluded that the best control for pre-emergence damping off was achieved by SAAF™ where seed treatment at the rate of 2 g per kg along with soil drenching at the rate of 2 g per liter and Vitavax^® as seed treatment at the rate of 2 g per kg along with soil drenching at the rate of 2 g per liter was used, while the best control of post-emergence damping off was achieved with combined seed treatment and soil treatment with SAAF™. There was no incidence of web blight throughout the growing period. Our findings could be useful in devising strategies for the integrated management of <i>R. solani</i> causing root rot disease in common beans.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full-Length Transcriptome Sequencing and Comparative Transcriptomics Reveal the Molecular Mechanisms Underlying Gonadal Development in Sleepy Cod (<i>Oxyeleotris lineolata</i>).","authors":"Jiajia Fan, Dongmei Ma, Huaping Zhu, Minghui Lin, Zaixuan Zhong, Yuanyuan Tian","doi":"10.3390/biology14030232","DOIUrl":"10.3390/biology14030232","url":null,"abstract":"<p><p>Sleepy cod (<i>Oxyeleotris lineolata</i>) is native to Australia and is now an economically valuable fish cultured in China and Southern Asian countries. Its growth rate exhibits as sexually dimorphic, with males generally growing more rapidly and attaining a larger body size compared to females. Thus, the effective development of sex control breeding can significantly contribute to increased yields and output value. Nevertheless, due to the lack of genomic and transcriptomic data, the molecular mechanisms underlying sex determination and gonadal differentiation in sleepy cod remain poorly understood. In this study, long-read PacBio isoform sequencing (Iso-Seq) was performed to obtain a full-length transcriptome from a pooled sample of eight tissues (kidney, brain, liver, muscle, heart, spleen, ovary and testis). A total of 30.41 G subread bases were generated and 49,113 non-redundant full-length transcripts with an average length of 2948 bp were produced. Using the full-length transcriptome as a reference, short-read Illumina sequencing was performed to investigate the differences in gene expression at the transcriptome level between ovaries and testes from 12-month-old individuals. A total of 19,102 differentially expressed transcripts (DETs) were identified, of which 8510 (44.55%) were up-regulated in the ovary and 10,592 (55.45%) were up-regulated in the testis. The DETs were mainly clustered into 241 KEGG pathways, in which oocyte meiosis and arachidonic acid metabolism were the most relevant pathways involved in gonadal differentiation. To verify the validity of the transcriptomic data, 20 DETs were selected to investigate the gonad expression profiles based on qPCR. The expression levels of all 20 screened genes were consistent with the transcriptome sequencing results. The present study provides new genetic resources-including full-length transcriptome sequences and annotation information-as a coding genomic-level reference for sleepy cod-yielding valuable insights into the genetic mechanisms of sex determination and gonadal differentiation in this economically important species.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11940265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zebrafish (<i>Danio rerio</i>) Prefer Undisturbed Shoals over Shoals Exposed to the Synthetic Alarm Substance Hypoxanthine-3N-oxide (C₅H₄N₄O₂).","authors":"Andrew Velkey, Kaitlyn Kinslow, Megan Bowers, Ethan Hoffman, Jamie Martin, Bandhavi Surisetty","doi":"10.3390/biology14030233","DOIUrl":"10.3390/biology14030233","url":null,"abstract":"<p><p>As an anti-predation behavior, shoaling enhances survival among prey species by reducing individual predation risk through mechanisms like the dilution effect and collective vigilance. Zebrafish-a highly social and genetically tractable species-are valuable for studying these behaviors. The present study examined zebrafish's social preferences in a 3-chamber open-tank free-swim task, assessing whether visual cues alone could distinguish between an intact and an alarmed shoal exposed to the synthetic alarm substance H3NO. Subjects were allowed to freely associate with either shoal while their behaviors were recorded and analyzed. The results reveal a significant preference for proximity to the intact shoal, indicating zebrafish's ability to visually discern threat levels. Subjects spent nearly twice as much time in the zone near the intact shoal, with reduced freezing and faster movement velocities compared to the alarmed shoal zone. Males exhibited more freezing behavior than females, consistent with sex-specific strategies in threat response. These findings underscore zebrafish's reliance on visual cues for social responding under predatory threat and highlight sex-based differences in threat perception. This research expands the understanding of zebrafish's social dynamics and provides a robust framework for future exploration of the neural mechanisms underlying social behavior and threat assessment in zebrafish.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Germplasm Improvement in Three Farmed Grass Carp Populations Based on Genetic Variability.","authors":"Zhongyuan Shen, Liming Shao, Xixi Liu, Haiqi Li, Haipeng Guo, Lang Qin, Kaikun Luo, Wuhui Li, Jing Wang, Shengnan Li, Qianhong Gu, Liang Guo, Xu Huang, Qinbo Qin, Shaojun Liu","doi":"10.3390/biology14030230","DOIUrl":"10.3390/biology14030230","url":null,"abstract":"<p><p>The assessment of genetic improvement was comprehensively analyzed using the mtDNA <i>Cyt b</i> gene and SSR markers among three farmed grass carp populations caught in May 2024. The results of an mtDNA <i>Cyt b</i> gene analysis in 198 individuals showed that the haplotype diversity index (<i>H_i</i>) and nucleotide diversity index (<i>P_i</i>) were 0.555 and 0.00058, respectively. The results of the analysis of SSR marker data in 196 individuals indicated that the unequal dosage amplification at the same locus was found in the CC population. Moreover, the total number of alleles (<i>A</i>: 338), number of alleles per locus (<i>Na</i>: 15.36), observed heterozygosity (<i>Ho</i>: 0.8391), expected heterozygosity (<i>He</i>: 0.8380), and polymorphic information content (<i>PIC</i>: 0.8191) in the KC population was relatively higher than that in the CC (<i>A</i>: 129; <i>Na</i>: 5.86; <i>Ho</i>: 0.0025; <i>He</i>: 0.6191; <i>PIC</i>: 0.5747) and CY (<i>A</i>: 293; <i>Na</i>: 8.77; <i>Ho</i>: 0.821; <i>He</i>: 0.7483; and <i>PIC</i>: 0.5747) populations. The <i>FST</i> and AMOVA analysis showed the existence of a significant differentiation (<i>p</i> < 0.001), with a high genetic differentiation between the CC and CY populations. In summary, a high genetic variability exists in the KC population, while the father (CY) and mother (CC) populations have relatively low genetic variability. This study reveals evidence of the existence of a \"micro-hybrid\". Moreover, the results demonstrated that combining both gynogenesis and backcross breeding technology is vital for the genetic improvement of grass carp. Moreover, continuous research into the genetic health of these populations is required as well as support for the protection of germplasm resources and artificial breeding.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Characterization of OFT and OFO Cell Lines from Olive Flounder (<i>Paralichthys olivaceus</i>) for Use as Feeder Cells.","authors":"Ja Young Jo, Ju-Won Kim, Eun Soo Noh, Yong-Ok Kim, Seung Pyo Gong, Hee Jeong Kong, Jae Hoon Choi","doi":"10.3390/biology14030229","DOIUrl":"10.3390/biology14030229","url":null,"abstract":"<p><p>Olive flounder (<i>Paralichthys olivaceus</i>) is a commercially important fish species in Japan, China, and the Republic of Korea. Despite numerous attempts to improve productivity, there have been no studies of in vitro germline stem cell (GSC) culture in this species. Here, olive flounder testicular and ovarian cell lines (OFT and OFO, respectively) were established and characterized. RT-PCR demonstrated that OFT and OFO expressed several gonadal somatic cell markers, including <i>wt1</i> and <i>fgf2</i>, but lacked expression of germ cell markers, such as <i>vasa</i>, <i>nanos2</i>, and <i>scp3</i>. In addition, SNP analysis revealed that OFT originated from XY male <i>P. olivaceus</i> and OFO originated from XX female <i>P. olivaceus</i>. These results suggest that OFT was composed of Sertoli cells and OFO was composed of granulosa cells and theca cells. Finally, coculture of OFT or OFO with enriched male <i>P. olivaceus</i> GSCs isolated from the top 20% and 20-30% Percoll density gradient layers showed that GSCs were attached on both cell lines. In conclusion, we established <i>P. olivaceus</i> testicular and ovarian cell lines, which were expected to use for development of an in vitro GSC culture system.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical Evaluation of Bone Remodeling Following Compressive Force on Mandibular Condyle.","authors":"Ioannis Lyros, Ioannis A Tsolakis, Georgia Kotantoula, Konstantinos Tosios, Vilaras George, Nikolaos Nikitakis, Efstratios Ferdianakis, Theodoros Lykogeorgos, Eleni Fora, Apostolos I Tsolakis","doi":"10.3390/biology14030228","DOIUrl":"10.3390/biology14030228","url":null,"abstract":"<p><p>Excessive mandibular growth is largely affected by genetic factors and its orthodontic management is challenging. The present study utilized histophotometry and aimed to investigate immunohistochemical alterations in rat condyles following posterior mandibular displacement using a fixed intraoral functional appliance. Seventy-two male Wistar rats were divided into two equal groups, experimental and control, each consisting of three equal subgroups. The animals were sacrificed after 30, 60, and 90 days, and their condyles were isolated and examined microscopically for potential immunohistochemical changes. Statistically significant differences in RANKL appeared at days 30 and 60, while noteworthy changes regarding TRAP were evidenced at days 30 and 90. In the experimental group, RANKL decreased statistically significantly between days 60 and 90, while in the control group, the RANKL measured significantly increased at day 90 compared to days 30 and 60 alike. In the experimental group, TRAP appeared significantly decreased at day 60 vs. 30, while at day 90 vs. 60, it was found to be significantly increased. In the control group, there were no significant changes in TRAP. Posterior mandibular displacement may cause immunohistochemical changes in the rat condylar bone, and such outcomes should be considered when planning similar procedures in humans.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 3","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11939411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}