Biology-Basel最新文献_第9页

Circulating Antimicrobial Peptides as Biomarkers of Inflammation and Airway Dysfunction After Marathon Running. 循环抗菌肽作为马拉松后炎症和气道功能障碍的生物标志物。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-07 DOI: 10.3390/biology14070825

Marie-Therese Lingitz, Hannes Kühtreiber, Lisa Auer, Michael Mildner, Claus G Krenn, Clemens Aigner, Bernhard Moser, Christine Bekos, Hendrik Jan Ankersmit

{"title":"Circulating Antimicrobial Peptides as Biomarkers of Inflammation and Airway Dysfunction After Marathon Running.","authors":"Marie-Therese Lingitz, Hannes Kühtreiber, Lisa Auer, Michael Mildner, Claus G Krenn, Clemens Aigner, Bernhard Moser, Christine Bekos, Hendrik Jan Ankersmit","doi":"10.3390/biology14070825","DOIUrl":"10.3390/biology14070825","url":null,"abstract":"Marathon running exerts physical stress and may lead to transient immune dysregulation, increasing susceptibility to airway inflammation and exercise-induced bronchoconstriction (EIB). This study investigated systemic levels of antimicrobial peptides in athletes and their association with EIB. Serum concentrations of angiogenin, human beta-defensin 2 (hBD-2), major basic protein (MBP), S100A8, and S100A8/A9 were measured in 34 marathoners and 36 half-marathoners at baseline, immediately after a race, and seven days postrace using enzyme-linked immunosorbent assays and compared with 30 sedentary controls. Lung function was assessed by spirometry to identify bronchoconstriction. Levels of hBD-2 and S100A8/A9 were significantly elevated postrace in runners compared to baseline and controls, returning to baseline during recovery. During recovery, S100A8 levels remained slightly elevated in marathoners with EIB. Similarly, human beta-defensin 2 was modestly increased in runners who developed bronchoconstriction. Notably, S100A8 levels correlated negatively with lung function parameters, including forced expiratory volume and mid-expiratory flows. These findings suggest that endurance running induces systemic inflammatory responses and modulates innate immune peptides, particularly in individuals prone to bronchoconstriction. These peptides may serve as biomarkers of respiratory stress and help guide personalized strategies in endurance sports.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Regulatory Role of EvfG Through Coordinated Control of Flagellar Biosynthesis and Energy Metabolism in Porcine Extraintestinal Pathogenic Escherichia coli (ExPEC). EvfG通过协调控制鞭毛生物合成和能量代谢对猪肠外致病性大肠杆菌的调节作用

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-07 DOI: 10.3390/biology14070822

Bingbing Zong, Peiyi Wang, Wei Liu, Aihua Wu, Yong Xiao, Shulin Fu, Yinsheng Qiu, Yanyan Zhang, Wentong Liu

{"title":"The Regulatory Role of EvfG Through Coordinated Control of Flagellar Biosynthesis and Energy Metabolism in Porcine Extraintestinal Pathogenic Escherichia coli (ExPEC).","authors":"Bingbing Zong, Peiyi Wang, Wei Liu, Aihua Wu, Yong Xiao, Shulin Fu, Yinsheng Qiu, Yanyan Zhang, Wentong Liu","doi":"10.3390/biology14070822","DOIUrl":"10.3390/biology14070822","url":null,"abstract":"In this study, we found that the deletion of the gene evfG in the type VI secretion system (T6SS) gene cluster significantly affected the motility of porcine extraintestinal pathogenic Escherichia coli (ExPEC) strain PCN033. Furthermore, the bacterial motility assay showed that ΔevfG mutants exhibited reduced motility compared to the parental strain. Transmission electron microscopy (TEM) showed a significant reduction in the number of flagella in the mutant ΔevfG when compared with PCN033. To further explore the reasons why the deletion of evfG affects the motility of PCN033, transcriptomic and metabolomic analyses were conducted. The omics analyses showed that 134 differentially accumulated metabolites and 2236 differentially expressed genes were identified between the mutant ΔevfG and the parental strain PCN033. The metabolome profile and functional annotation analyses indicated that the impaired motility of ΔevfG was connected to the downregulation of the expression levels of genes associated with the energy metabolism pathway and flagellar assembly. Our study provides a new insight into the diminished PCN033 motility induced by evfG deletion. Moreover, the candidate genes and metabolites regulated by the gene evfG in the T6SS, which was involved in the motility of PCN033, were reported in this study.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Role of Spicules in Shaping Sponge Body Structure: Evidence from the Early Cambrian Shuijingtuo Formation. 揭示针状体在形成海绵体结构中的作用：来自早寒武世水井沱组的证据。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-07 DOI: 10.3390/biology14070826

Xinyi Ren, Yazhou Hu, Luke C Strotz, Mei Luo, Caibin Zhang, Zhifei Zhang

{"title":"Unraveling the Role of Spicules in Shaping Sponge Body Structure: Evidence from the Early Cambrian Shuijingtuo Formation.","authors":"Xinyi Ren, Yazhou Hu, Luke C Strotz, Mei Luo, Caibin Zhang, Zhifei Zhang","doi":"10.3390/biology14070826","DOIUrl":"10.3390/biology14070826","url":null,"abstract":"In most cases, sponge fossils are preserved as isolated spicules, with complete sponge body fossils largely confined to Konservat-Lagerstätten. Although the classification and diversity of sponges and their isolated spicules have been extensively studied, no systematic attempts have been made to define the relationship between fossil spicules and the sponge body plan. By utilizing relatively well-preserved sponge fossils from the black shales of the Shuijingtuo Formation (South China) in conjunction with isolated spicules from the same locality, we assess spicule morphology to identify the potential functional roles of spicules and chart their arrangement within the sponge body. The elemental distribution and three-dimensional morphology of the examined sponge body fossil (likely a hexactinelid) are assessed using both micro-XRF and micro-CT. Tetractine, stauractine and pentactine spicules are the most abundant spicule types, both in the body fossil and in acid residues, with an additional spicule type (monaxons) also present. The larger pentactine spicules (five-ray spicules) frame the structure, whereas the smaller tetractines and stauractines (four-ray spicules), along with smaller pentactines, are arranged along the branches of the larger spicules. Based on the arrangement of the different spicules, it is proposed that each of the spicule types represents a discrete functional form: monaxons support the overall sponge body plan, pentactines construct the framework of the parietal gaps, and the smaller pentactines or tetractines stabilize the framework of the parietal gaps. These results provide a new understanding of sponge morphology, spicule function and the relationship between isolated fossil spicules and associated sponge body fossils.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Environmental DNA Metabarcoding and Underwater Visual Census for Assessing Macrobenthic Diversity. 环境DNA元条形码与水下视觉普查评估大型底栖生物多样性的比较。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-06 DOI: 10.3390/biology14070821

Zifeng Zhan, Weiwei Huo, Shangwei Xie, Wandong Chen, Xinming Liu, Kuidong Xu, Yanli Lei

{"title":"Comparison of Environmental DNA Metabarcoding and Underwater Visual Census for Assessing Macrobenthic Diversity.","authors":"Zifeng Zhan, Weiwei Huo, Shangwei Xie, Wandong Chen, Xinming Liu, Kuidong Xu, Yanli Lei","doi":"10.3390/biology14070821","DOIUrl":"10.3390/biology14070821","url":null,"abstract":"The rapid advancement of environmental DNA (eDNA) technology has transformed ecological research, particularly in aquatic ecosystems. However, the optimal sampling matrix (e.g., water or sediment) and the potential for eDNA to replace or complement traditional underwater visual census (UVC) remain unclear. Here, we integrate water eDNA, sediment eDNA, and UVC approaches to systematically compare the diversity of benthic macrofauna in the subtidal zones of the Nanji Islands, China. Our results show that sediment eDNA samples exhibited the highest species richness, while UVC had the lowest. Each method revealed distinct species profiles, with relatively few shared taxa at the order level and below. Environmental eDNA showed significant advantages in detecting key phyla such as Annelida and Arthropoda. In contrast, traditional UVC was crucial for identifying certain taxa, such as Bryozoa, which were undetectable by eDNA methods. The low overlap in species detected by these methods underscores their complementary nature, highlighting the necessity of integrating multiple approaches to achieve a more comprehensive and accurate biodiversity assessment. Future research should focus on refining eDNA techniques, such as developing more universal primers, to further enhance their applicability in biodiversity monitoring.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybrid Deep Learning Framework for High-Accuracy Classification of Morphologically Similar Puffball Species Using CNN and Transformer Architectures. 基于CNN和Transformer架构的形态相似Puffball物种高精度分类混合深度学习框架。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-05 DOI: 10.3390/biology14070816

Eda Kumru, Güney Ugurlu, Mustafa Sevindik, Fatih Ekinci, Mehmet Serdar Güzel, Koray Acici, Ilgaz Akata

{"title":"Hybrid Deep Learning Framework for High-Accuracy Classification of Morphologically Similar Puffball Species Using CNN and Transformer Architectures.","authors":"Eda Kumru, Güney Ugurlu, Mustafa Sevindik, Fatih Ekinci, Mehmet Serdar Güzel, Koray Acici, Ilgaz Akata","doi":"10.3390/biology14070816","DOIUrl":"10.3390/biology14070816","url":null,"abstract":"Puffballs, a group of macrofungi belonging to the Basidiomycota, pose taxonomic challenges due to their convergent morphological features, including spherical basidiocarps and similar peridial structures, which often hinder accurate species-level identification. This study proposes a deep learning-based classification framework for eight ecologically and taxonomically important puffball species: Apioperdon pyriforme, Bovista plumbea, Bovistella utriformis, Lycoperdon echinatum, L. excipuliforme, L. molle, L. perlatum, and Mycenastrum corium. A balanced dataset of 1600 images (200 per species) was used, divided into 70% training, 15% validation, and 15% testing. To enhance generalizability, images were augmented to simulate natural variability in orientation, lighting, and background. In this study, five different deep learning models (ConvNeXt-Base, Swin Transformer, ViT, MaxViT, EfficientNet-B3) were comparatively evaluated on a balanced dataset of eight puffball species. Among these, the ConvNeXt-Base model achieved the highest performance, with 95.41% accuracy, and proved especially effective in distinguishing morphologically similar species such as Mycenastrum corium and Lycoperdon excipuliforme. The findings demonstrate that deep learning models can serve as powerful tools for the accurate classification of visually similar fungal species. This technological approach shows promise for developing automated mushroom identification systems that support citizen science, amateur naturalists, and conservation professionals.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy Metal-Associated (HMA) Domain-Containing Proteins: Insight into Their Features and Roles in Bread Wheat (Triticum aestivum L.). 面包小麦（Triticum aestivum L.）中重金属相关（HMA）结构域蛋白的特征和作用

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-05 DOI: 10.3390/biology14070818

Mehak Taneja, Santosh Kumar Upadhyay

{"title":"Heavy Metal-Associated (HMA) Domain-Containing Proteins: Insight into Their Features and Roles in Bread Wheat (Triticum aestivum L.).","authors":"Mehak Taneja, Santosh Kumar Upadhyay","doi":"10.3390/biology14070818","DOIUrl":"10.3390/biology14070818","url":null,"abstract":"The heavy metal-associated (HMA) domain-harboring proteins constitute critical players involved in the transport of various metal ions in plants, and are associated with development and stress responses. Herein, a total of 243 TaHMA genes were identified in the bread wheat genome, each of which had a characteristic molecular profile and a distinct chromosomal localization. The TaHMA proteins were distributed in five clades in phylogeny, which differed with respect to the distribution of the key HMA domain. Sub-cellular localization was variable for the TaHMA proteins. Gene structure analysis yielded similar results when compared with the orthologous counterparts. Cis-regulatory element analysis produced a range of promoter elements, suggesting their diverse biological roles. Gene duplication analysis revealed a crucial role played by tandem and segmental duplication events in the expansion of TaHMA genes. Synteny analysis highlighted the evolutionary relatedness of TaHMA genes with those derived from Arabidopsis and rice. Expression analysis provided crucial information about the role of TaHMAs in mediating vital responses in the plant body, including the development of tissues and the regulation of various abiotic stress conditions. Overall, the study provides significant cues and evidence to functionally annotate and characterize the differentially expressed TaHMAs in order to validate their role.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Lentiviral Reporter System for In Vitro Reprogramming of Astrocytes to Neuronal Precursors. 星形胶质细胞体外重编程为神经元前体的慢病毒报告系统的建立。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-05 DOI: 10.3390/biology14070817

Anna Schnaubelt, Guoli Zheng, Maryam Hatami, Johannes Tödt, Hao Wang, Thomas Skutella, Andreas Unterberg, Klaus Zweckberger, Alexander Younsi

{"title":"Development of a Lentiviral Reporter System for In Vitro Reprogramming of Astrocytes to Neuronal Precursors.","authors":"Anna Schnaubelt, Guoli Zheng, Maryam Hatami, Johannes Tödt, Hao Wang, Thomas Skutella, Andreas Unterberg, Klaus Zweckberger, Alexander Younsi","doi":"10.3390/biology14070817","DOIUrl":"10.3390/biology14070817","url":null,"abstract":"Astrocytes, which proliferate after brain injury, represent a promising target for cellular reprogramming due to their abundance and ability to support brain repair. In this study, we investigated the in vitro reprogramming of primary cortical astrocytes from neonatal rats into neuronal precursor cells (NPCs) using the transcription factors Oct4, Sox2, and Klf4 (OSK), delivered via lentiviral vectors. We designed a reporter system to trace the conversion of astrocytes to NPCs and neurons by using GFAP-driven iCre and Nestin- or Synapsin1-driven fluorescent reporters. After transduction, we observed morphological changes and the expression of neuronal markers in some cells, while many cells remained in a transitional state, expressing both astrocytic and neuronal features. Importantly, the study was not designed to quantify reprogramming efficiency or demonstrate full astrocyte-to-neuron conversion but rather to establish and evaluate a traceable reporter system. Our data suggest that OSK-mediated reprogramming in this in vitro model can initiate conversion of astrocytes to neuronal precursor-like cells, although the process is complex and incomplete within the one-week timeframe. We also highlight limitations in co-transduction efficiency and potential silencing of the reporter system during reprogramming. These findings provide an initial technical platform to explore astrocyte reprogramming in vitro and inform future studies aiming to refine these methods and apply them in vivo.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DIRAS1 Drives Oxaliplatin Resistance in Colorectal Cancer via PHB1-Mediated Mitochondrial Homeostasis. 通过phb1介导的线粒体稳态，DIRAS1驱动结直肠癌奥沙利铂耐药。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-05 DOI: 10.3390/biology14070819

Min Long, Qian Ouyang, Jingyi Wen, Xuan Zeng, Zihui Xu, Shangwei Zhong, Changhao Huang, Jun-Li Luo

{"title":"DIRAS1 Drives Oxaliplatin Resistance in Colorectal Cancer via PHB1-Mediated Mitochondrial Homeostasis.","authors":"Min Long, Qian Ouyang, Jingyi Wen, Xuan Zeng, Zihui Xu, Shangwei Zhong, Changhao Huang, Jun-Li Luo","doi":"10.3390/biology14070819","DOIUrl":"10.3390/biology14070819","url":null,"abstract":"Background: Colorectal cancer (CRC) is a prevalent global malignancy with particularly challenging treatment outcomes in advanced stages. Oxaliplatin (OXA) is a frontline chemotherapeutic agent for CRC. However, 15% to 50% of stage III patients experience recurrence due to drug resistance. Elucidating the molecular mechanisms underlying OXA resistance is, therefore, crucial for improving CRC prognosis. The role of DIRAS1, a RAS superfamily member with reported tumor-suppressive functions in various cancers, remains poorly defined in CRC.Methods: The effects of DIRAS1 on CRC cell proliferation and migration were evaluated using MTT, wound healing, and colony formation assays. Stable cell lines with knockdown or overexpression of DIRAS1 and PHB1 were established via plasmid and lentiviral systems. Drug sensitivity to OXA was assessed through cytotoxicity assays and IC50 determination. Clinical relevance was validated through immunohistochemical analysis of CRC tissue samples. Transcriptomic sequencing was performed to explore downstream regulatory mechanisms.Results: DIRAS1 expression was positively correlated with OXA resistance and was significantly upregulated following prolonged chemotherapy exposure. Silencing DIRAS1 reduced the IC50 of OXA in vitro and increased tumor sensitivity to OXA in vivo. Transcriptome analysis identified PHB1 as a downstream effector of DIRAS1. Functional studies revealed that PHB1 contributes to chemoresistance by maintaining mitochondrial stability.Conclusions: This study identifies DIRAS1 as a key contributor to OXA resistance in CRC by modulating PHB1 expression and mitochondrial function. Targeting the DIRAS1-PHB1 axis may offer a novel therapeutic strategy to overcome chemoresistance in CRC.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Bacillus subtilis and Rhodotorula Yeast Culture on the Growth Performance, Meat Quality, Antioxidant Capacity, and Serum Metabolites in Yellow-Feathered Broilers. 枯草芽孢杆菌和酵母红酵母培养物对黄羽肉鸡生长性能、肉品质、抗氧化能力和血清代谢物的影响

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-05 DOI: 10.3390/biology14070820

Ke Wang, Xiangtan Su, Xinyu Lu, Guang Yang, Gaowei Zhang, Jiwei Chen, Jiale Sun, Aiqin Gao

{"title":"Effects of Bacillus subtilis and Rhodotorula Yeast Culture on the Growth Performance, Meat Quality, Antioxidant Capacity, and Serum Metabolites in Yellow-Feathered Broilers.","authors":"Ke Wang, Xiangtan Su, Xinyu Lu, Guang Yang, Gaowei Zhang, Jiwei Chen, Jiale Sun, Aiqin Gao","doi":"10.3390/biology14070820","DOIUrl":"10.3390/biology14070820","url":null,"abstract":"This study aimed to investigate the effects of dietary supplementation with Bacillus subtilis (BS) and Rhodotorula yeast culture (RYC) used as feed additives to study their effects on growth performance, meat quality, antioxidant capacity, and serum metabolomics in yellow-feathered broilers. A total of 192 one-day-old healthy yellow-feathered broilers were randomly divided into four treatment groups. The CON group was fed a basal diet, whereas the BS, RYC, and MIX groups were fed diets supplemented with 5 × 109 CFU/kg BS, 5000 mg/kg RYC, and a combination of both, respectively. The experimental period comprised 56 days. In the initial stage of growth, compared with the CON group, the body weight and average daily gain of broilers in the BS group were significantly increased (p < 0.05). The BS group increased the activity of CAT and SOD in broilers at 28 days, and the activity of CAT in RYC and MIX group were also significantly increased (p < 0.05). BS could increase GSH-Px activity significantly in broilers at 56 days (p < 0.05). Moreover, in comparison with the CON group, the MIX group decreased shear force and drip loss significantly (p < 0.05). Serum metabolome analysis showed that the addition of BS or RYC alone had the greatest difference in the abundance of D-amino acid metabolism and lysine metabolism, while the combined addition of BS and RYC found that glycerophospholipid metabolism and tryptophan metabolism had the greatest difference. In conclusion, dietary addition of BS and RYC could improve the meat quality of yellow-feathered broilers.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ScnR1-Mediated Competitive DNA Binding and Feedback Inhibition Regulate Guvermectin Biosynthesis in Streptomyces caniferus. scnr1介导的竞争性DNA结合和反馈抑制调节cancanus链霉菌Guvermectin的生物合成。

IF 3.5 3区生物学

Biology-Basel Pub Date : 2025-07-04 DOI: 10.3390/biology14070813

Haoran Shi, Jiabin Wang, Xuedong Zhang, Na Zhou, Xiangjing Wang, Wensheng Xiang, Shanshan Li, Yanyan Zhang

{"title":"ScnR1-Mediated Competitive DNA Binding and Feedback Inhibition Regulate Guvermectin Biosynthesis in Streptomyces caniferus.","authors":"Haoran Shi, Jiabin Wang, Xuedong Zhang, Na Zhou, Xiangjing Wang, Wensheng Xiang, Shanshan Li, Yanyan Zhang","doi":"10.3390/biology14070813","DOIUrl":"10.3390/biology14070813","url":null,"abstract":"Guvermectin, a Streptomyces-derived purine nucleoside compound, exhibits dual bioactivities as a plant growth regulator and an antibacterial agent. While its biosynthetic gene cluster (BGC) is regulated by the cluster-situated activator GvmR and the adjacent repressor GvmR2, the role of distal transcriptional regulators (TRs) in guvermectin biosynthesis remains unexplored. Here, we identified ScnR1, a highly conserved LacI-family TR located far from the guvermectin BGC, which is directly activated by GvmR. Overexpression of scnR1 significantly suppressed guvermectin biosynthesis. Further investigations revealed that ScnR1 competitively binds to the gvmR, gvmA, and O1 promoters (overlapping with the GvmR-binding sites), thereby inhibiting the guvermectin BGC transcription. Moreover, ScnR1 formed a reciprocal feedback loop with the adjacent repressor GvmR2, where each repressor inhibits the other's expression. These findings reveal a multi-layered regulatory mechanism wherein LacI-family TRs fine-tune guvermectin biosynthesis through competitive DNA binding and reciprocal feedback control. This study offers new perspectives on the hierarchical control of secondary metabolism in Streptomyces and provides valuable theoretical guidance for the engineering of strains with enhanced natural product production.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12292694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0