Biology-Basel最新文献

{"title":"Comprehensive Ubiquitome Analysis of <i>Nicotiana benthamiana</i> Leaves Infected with Tomato Brown Rugose Fruit Virus.","authors":"Jiali Yang, Donghai Wang, Boshen Zhang, Mangle Chen, Jianping Chen, Fei Yan, Shaofei Rao","doi":"10.3390/biology14060656","DOIUrl":"10.3390/biology14060656","url":null,"abstract":"<p><p>Tomato brown rugose fruit virus (ToBRFV) is an important emerging virus that poses a serious threat to the global agricultural economy. Ubiquitination is one of the key post-translational protein modification types in plant responses to biotic stress, but the extent to which ToBRFV infection alters the overall ubiquitination status has not been reported. This study conducted integrated ubiquitome and proteome analyses of <i>Nicotiana benthamiana</i> leaves infected with ToBRFV and identified differentially ubiquitinated proteins. A total of 346 lysine sites on 302 identified proteins were found to be affected, with 260 sites exhibiting upregulated ubiquitination levels in 224 proteins and 86 sites showing downregulated ubiquitination levels in 80 proteins. The differentially ubiquitinated proteins were primarily localized in the cytoplasm (29%), nucleus (18%), plasma membrane (8.9%), mitochondria (5.1%), and chloroplasts (4.6%). Fourteen conserved ubiquitination motifs, including ENNNK, ENNK, SK, and KNG, were identified. Furthermore, enrichment analysis indicated that ToBRFV infection induces an increase in the ubiquitination levels of proteins associated with ion transport, MAPK signaling pathways, and plant hormone signal transduction, while the ubiquitination levels of proteins related to carbon metabolism and secondary metabolite synthesis decreased. Functional analysis of the three differentially ubiquitinated proteins revealed that a RING/U-box superfamily protein negatively regulates ToBRFV infection. Our work provides the first systematic analysis of the ubiquitination profile in <i>N. benthamiana</i> leaves following ToBRFV infection, providing important resources for further studies on the regulatory mechanisms of ubiquitination in plant responses to ToBRFV.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Lethal Effects of Combined P-tert-Butylcatechol and L-Lysine on <i>Microcystis aeruginosa</i>.","authors":"Heyun Jiao, Gangwei Jiao, Ruitong Jiang, Yifei Shen, Peimin He, Liu Shao","doi":"10.3390/biology14060655","DOIUrl":"10.3390/biology14060655","url":null,"abstract":"<p><p>Allelochemicals are recognized as promising algaecides due to their environmental safety. Para-tert-butylcatechol (TBC) and L-lysine exhibit significant potential in suppressing harmful algal blooms (HABs); however, their combined effects and algae inhibition mechanisms remain unelucidated. Therefore, this study systematically investigated the growth inhibition of <i>Microcystis aeruginosa</i> by TBC and L-lysine individually and in combination, while simultaneously examining their combined effects on algal growth, cell membrane integrity, photosynthetic activity, antioxidant responses, and microcystin production. The results revealed a significant interactive effect between TBC (0.04 mg/L) and L-lysine (1 mg/L), achieving over 90% growth inhibition within 96 h. The combined treatment significantly inhibited <i>M. aeruginosa</i> growth through impaired photosynthetic efficiency and elevated oxidative stress. Compared to the control group, the treatment group exhibited a continuous decline in chlorophyll-a content, phycobiliprotein levels, <i>F_v</i>/<i>F_m</i>, YII, α, and rETR_max, while phosphoenolpyruvate carboxylase (PEPC) activity decreased by 96.48% by day 8. And antioxidant enzymes, including superoxide dismutase (SOD) and reduced glutathione (GSH), showed a progressive increase in activity. In addition, the structure and integrity of the cell membrane of <i>M. aeruginosa</i> were damaged after treatment, and the conductivity of the treatment groups increased continuously from 2.32 to 4.63 μs/cm. In addition, under combined treatment, intra- and extracellular microcystin levels initially increased (peaking at day 2) but sharply declined thereafter, becoming significantly lower than controls by day 8. These findings highlight the potential of combining TBC and L-lysine as an eco-friendly and cost-effective strategy for mitigating <i>M. aeruginosa</i>-dominated harmful algal blooms.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Evolution of White Spot Syndrome Virus in Shrimp: Insights from Transposon Dynamics.","authors":"Zhouquan Li, Guanghua Huang, Jingyi Zhang, Mingyou Li, Zhizhi Liu, Sihua Peng, Rui Wang, Dong Liu","doi":"10.3390/biology14060653","DOIUrl":"10.3390/biology14060653","url":null,"abstract":"<p><p>White spot syndrome virus (WSSV) has emerged as a significant threat to global shrimp aquaculture, causing economic losses because of its rapid spread and high mortality rates. This study aims to elucidate the genetic and evolutionary dynamics of WSSV through a comprehensive genome analysis. Utilizing 27 complete genome sequences sourced from public databases, this study investigates the genetic variability, potential recombination events, and evolutionary patterns of WSSV. Our results identified multiple genomic deletions, 14 novel single-nucleotide polymorphism sites, and variable number tandem repeats across different strains, underscoring the virus's genetic diversity. A recombination event between freshwater and marine strains highlights a complex transmission pathway, potentially facilitated by aquaculture practices. A phylogenetic tree constructed using ancestral genes suggests that WSSV originated in Southeast Asia and subsequently globally spread, influenced by both natural and anthropogenic factors. Genomic shrinkage of the virus occurred in time series, while the host's viral infection induced transposon transposition and insertion into the earlier virus genome to provide a basis for genomic shrinkage. Our research emphasizes the importance of advanced molecular characterization and evolutionary models of the virus in understanding the spread of viral pathogens in aquaculture environments.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-Driven Transcriptome Prediction in Human Pathology: From Molecular Insights to Clinical Applications.","authors":"Xiaoya Chen, Huinan Xu, Shengjie Yu, Wan Hu, Zhongjin Zhang, Xue Wang, Yue Yuan, Mingyue Wang, Liang Chen, Xiumei Lin, Yinlei Hu, Pengfei Cai","doi":"10.3390/biology14060651","DOIUrl":"10.3390/biology14060651","url":null,"abstract":"<p><p>Gene expression regulation underpins cellular function and disease progression, yet its complexity and the limitations of conventional detection methods hinder clinical translation. In this review, we define \"predict\" as the AI-driven inference of gene expression levels and regulatory mechanisms from non-invasive multimodal data (e.g., histopathology images, genomic sequences, and electronic health records) instead of direct molecular assays. We systematically examine and analyze the current approaches for predicting gene expression and diagnosing diseases, highlighting their respective advantages and limitations. Machine learning algorithms and deep learning models excel in extracting meaningful features from diverse biomedical modalities, enabling tools like PathChat and Prov-GigaPath to improve cancer subtyping, therapy response prediction, and biomarker discovery. Despite significant progress, persistent challenges-such as data heterogeneity, noise, and ethical issues including privacy and algorithmic bias-still limit broad clinical adoption. Emerging solutions like cross-modal pretraining frameworks, federated learning, and fairness-aware model design aim to overcome these barriers. Case studies in precision oncology illustrate AI's ability to decode tumor ecosystems and predict treatment outcomes. By harmonizing multimodal data and advancing ethical AI practices, this field holds immense potential to propel personalized medicine forward, although further innovation is needed to address the issues of scalability, interpretability, and equitable deployment.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pro-Arrhythmic Effect of Chronic Stress-Associated Humoral Factors in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.","authors":"Shi Su, Jinglei Sun, Suhua Qiu, Wenting Wu, Jiali Zhang, Yi Wang, Chenxia Shi, Yanfang Xu","doi":"10.3390/biology14060652","DOIUrl":"10.3390/biology14060652","url":null,"abstract":"<p><p>Under chronic stress, the pro-arrhythmic effect and mechanism of circulating humoral factors in human cardiomyocytes remain unknown. In the present study, we observed the effect of serum from chronic-stress mice on the electrical activity of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Male C57/BL6J mice were subjected to 35 days of chronic unpredictable mild stress (CUMS). The serum from CUMS mice induced arrhythmia-like events (cell arrhythmias) in hiPSC-CMs in a time- and concentration-dependent manner. Patch clamp recordings in the heterologous expression system demonstrated that the serum derived from CUMS mice exerted an inhibitory effect on the cloned human potassium currents (<i>I</i>_to, <i>I</i>_Kr, <i>I</i>_Ks) that mediate action potential repolarization. In addition, serum from CUMS reduced the expression of relevant channel proteins. Moreover, both heat-inactivated serum and deproteinized serum evoked similar severity of cell arrhythmias in hiPSC-CMs as the untreated serum, indicating that circulating substances with small molecules were mainly involved in the occurrence of arrhythmias. Furthermore, metabolomics analysis showed that 90 small-molecule metabolites increased and 390 decreased in CUMS serum. We concluded that circulating humoral substances under chronic stress conditions have direct arrhythmogenic effects by inducing ion channel dysfunction in myocardial cells.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Microalgae's Physiological Responses to Fibrous and Layered Clay Minerals.","authors":"Zhongquan Jiang, Tianyi Wei, Sijia Wu, Zhongyang Wang, Zhonghua Zhao, Lu Zhang, Ying Ge, Zhen Li","doi":"10.3390/biology14060647","DOIUrl":"10.3390/biology14060647","url":null,"abstract":"<p><p>Microalgae interact with mineral particles in an aqueous environment, yet how clay minerals affect physiological processes in algal cells remains unexplored. In this study, we compared the effects of palygorskite (Pal) and montmorillonite (Mt), which respectively represent fibrous and layered clay minerals, on the physiological processes of <i>Chlamydomonas reinhardtii</i>. It was observed that <i>C. reinhardtii</i> responded differently to the treatments of Pal and Mt. The Pal particles bound tightly to and even inserted themselves into cells, resulting in a significant decrease in cell numbers from 27.35 to 21.02 × 10⁷ mL^-1. However, Mt was only loosely attached to the cell surface. The photosynthesis in the algal cells was greatly inhibited by Pal, with the rETR_max significantly reduced from 103.80 to 56.67 μmol electrons m^-2s^-1 and the downregulation of IF2CP, psbH and OHP1, which are key genes involved in photosynthesis. In addition, Pal reduced the quantities of proteins and polysaccharides in extracellular polymeric substances (EPSs) and the P uptake by <i>C. reinhardtii</i> when the P level in the culture was 3.15 mg/L. However, no significant changes were found regarding the above EPS components or the amount of P in algal cells upon the addition of Mt. Together, the impacts of fibrous Pal on <i>C. reinhardtii</i> was more profound than those of layered Mt.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Assessment and Trading Mechanism of Carbon Sink in China's Marine Aquaculture.","authors":"Xuan Yu, Haonan Guo, Qi Chen","doi":"10.3390/biology14060648","DOIUrl":"10.3390/biology14060648","url":null,"abstract":"<p><p>This study develops species-specific assessment models for carbon sink in marine aquaculture (CSMA) using provincial data from China's coastal regions (2004-2023). Key findings are as follows: (1) Cumulative CSMA reached 46.3618 million tonnes, exhibiting three growth phases-initial fluctuations (2004-2008), rapid growth (2008-2015), and optimization and maturation (2015-2023). (2) Species contributions were heterogeneous: shellfish dominated at 45%, followed by shrimp (24%), fish (15%), crab (11%), and algae (5%). (3) Spatially, Guangdong, Fujian, and Shandong consistently lead in CSMA; Guangxi, Liaoning, and Zhejiang form a second tier, whereas Hebei, Hainan, and Jiangsu remain at the lower end. (4) Province-specific dominance patterns were observed: shellfish-dominant pattern in Shandong, Fujian, and Liaoning; shrimp-dominant pattern in Hebei and Hainan; shellfish-and-shrimp dual-cores in Guangdong and Guangxi; and a multifaceted profile in Jiangsu and Zhejiang. To facilitate the realization of CSMA's value, we propose a dedicated trading mechanism. Based on the derivations from the effect analysis model and the illustrative case studies, we explore the potential economic and ecological benefits of CSMA trading.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of C-Jun N-Terminal Kinases on a Stressed Epithelium: Time for Testing Isoform Specificity.","authors":"Nitesh Shashikanth, Osama Alaidi, Lohitha Basa, Shreya Taank, RadhaKrishna Rao, Jayaraman Seetharaman","doi":"10.3390/biology14060649","DOIUrl":"10.3390/biology14060649","url":null,"abstract":"<p><p>Biological, physiological, and psychological stressors cause a \"stress response\" in our bodies. Stressors that are sensorily perceived (either acute or chronic) trigger hormonal responses from the sympathetic nervous system-the SAM and HPA axis-that effect intended organs to alert the individual. Other stressors have a direct effect on the target organ(s) of the body-e.g., physical injury and wounds, toxins, ionizing, and UV radiation. Both kinds of stressors change cell equilibrium, often leading to reactive oxygen species (ROS) accumulation and cellular damage. Among the signaling pathways involved in fighting these stressors, the c-Jun-N-terminal kinases (JNK) respond to diverse kinds of stressors. This review focuses on JNK1 and JNK2, both of which are ubiquitously present in all cell types, and attention is paid to gastrointestinal tract epithelial cells and their response-including tight junction disruption and cytoskeletal changes. We discuss the seemingly opposite roles of JNK1 and JNK2 in helping cells choose pro-survival and pro-apoptotic pathways. We examine the common features of the JNK protein structure and the possibilities of discovering JNK-isoform-specific inhibitors since, although JNK1 and JNK2 are involved in multiple diseases, including cancer, obesity, diabetes, musculoskeletal and liver disease, no cell-specific or isoform-specific inhibitors are available.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Preclinical Investigation of Estrogenic Bone Protection in a Hypertensive Rat Model Under Gender-Affirming Hormone Therapy.","authors":"Lucas Streckwall, Germán A Colareda, Daiana Escudero, Romina G Diaz, Juan M Fernández","doi":"10.3390/biology14060650","DOIUrl":"10.3390/biology14060650","url":null,"abstract":"<p><p>The goal of gender-affirming hormone therapy (GAHT) is to align an individual's physical characteristics with their gender identity by suppressing endogenous sex hormones and replacing them with those consistent with their gender. Transgender women undergoing GAHT are at higher risk of cardiovascular complications, and since clinical evidence suggests that hypertension is associated with increased bone loss, we investigated the effects of estrogen treatment on bone health in a hypertensive transgender animal model. Male spontaneously hypertensive rats were orchiectomized (Orch), and half of them received estrogen treatment (Orch + Es), while a third group remained intact as controls. Bone marrow progenitor cells (BMPCs) were isolated to assess osteogenic potential, and femurs were collected for histological and mechanical analysis. BMPCs from Orch + Es rats exhibited enhanced osteogenic potential compared to those from Orch rats. Histological analysis revealed a higher number of osteocytes and fewer adipocytes in the Orch + Es group. Mechanical testing showed reduced bone strength in Orch rats, which was partially preserved in Orch + Es animals. In conclusion, estrogen administration mitigated the deleterious effects of testosterone depletion on BMPCs and provided protective effects on bone structure and strength in this preclinical model of GAHT in hypertensive rats.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the Behavioral Characteristics of <i>Lutjanus erythropterus</i> on the Basis of the Distance and Structure of Artificial Reefs.","authors":"Manju Jiang, Yu Guo, Chuanxin Qin, Jia Zhang, Ankai Zhang","doi":"10.3390/biology14060644","DOIUrl":"10.3390/biology14060644","url":null,"abstract":"<p><p>The aims of this study were to analyze the preferences of rocky reef fish for different types of artificial reefs and to determine the distances between reefs that lead to the production of independent effects. In this study, the aggregation behavior of juvenile <i>Lutjanus erythropterus</i> was observed under different reef spacings (0.5 L (distances of 0.5 times the reef length), 1.0 L, 1.5 L, 2.0 L, 2.5 L, and 3.0 L). The results revealed that when the distance between the reefs was 0.5 L, the juvenile fish presented frequent between-reef activity, and their attraction to the reefs was significant. When the distance between the reefs was 2.5 L or 3.0 L, the number of juvenile fish appeared to significantly differ between the areas. The number of juvenile fish farther from the reefs was relatively low, and the value tended to plateau. The results revealed that the two reefs had a combined effect when the reef distance was 0.5 L, 1.0 L, 1.5 L, or 2.0 L, whereas the two reefs had independent effects when the distance was 2.5 L or 3.0 L. In addition, to determine the distance at which the reefs had independent effects, the preference of juvenile <i>L. erythropterus</i> for different reef types was investigated, and the behavior of <i>L. erythropterus</i> was observed when two or four reefs (room-shaped artificial reef, RAR; lobe-shaped artificial reef, LAR; cubic artificial reef, CAR; and stacked artificial reef, SAR) were placed in an experimental tank at the same time. The results showed that in the experiments where two or four different reef models were present, juvenile <i>L. erythropterus</i> preferred the cubic artificial reef. At this reef, the fish exhibited the strongest reefing behavior and stayed for the longest time. This study reveals the reefing behaviors of juvenile <i>L. erythropterus</i> under different reef spacings and their preferences for various artificial reef models, providing a theoretical basis and reference for the design and selection of habitats for related species and of artificial reef models for marine pastures.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}