Biology-Basel最新文献

{"title":"Application of Biochar on Soil Improvement and Speciation Transformation of Heavy Metal in Constructed Wetland.","authors":"Yuan Zhou, Xiaoqin Nie, Yao Zhao, Liqiu Zhang, Yatian Cheng, Cancan Jiang, Wenbin Zhao, Xiangchun Wang, Chao Yang","doi":"10.3390/biology14050515","DOIUrl":"10.3390/biology14050515","url":null,"abstract":"<p><p>The pyrolysis of sewage sludge into biochar, enhanced by incorporating agriculture waste rich in inorganic minerals and lignocellulosic compounds, provides an effective approach for achieving sludge-harmless treatment and resourceful utilization. In this study, sewage sludge and maize straw-based biochar (SMB) was prepared using the co-pyrolysis method, and the effects of different application ratios (0%, 1%, 3%, and 5%, <i>w</i>/<i>w</i>) of SMB on soil properties, ryegrass growth, microbial community structure, and Pb content and speciation in the contaminated soil of constructed wetlands were investigated. The results showed that SMB had a high carbon content (28.58%) and was rich in functional groups (e.g., -C-O, -C-N). The results indicated that increasing SMB dosage (0-5% <i>w</i>/<i>w</i>) in Pb-contaminated soil elevated soil pH from 6.40 to 7.93, cation-exchange capacity (CEC) from 30.59 to 79.03 cmol/kg (+158%), and organic carbon content by 65% (from 176.79 mg/kg to 107.3 mg/kg), while reducing available phosphorus and potassium by 20% and 30%, respectively, resulting in a 6% decline in ryegrass leaf length. SMB application enriched Pb-resistant bacteria (e.g., <i>Sphingomonas</i> abundance increased from 10.3% to 11.2%) and enhanced Pb immobilization. After 55 days, the total soil Pb increased by 33%, and the residual fraction Pb significantly increased by 7.3% to 21.7%, driven by functional group complexation, ion exchange, pH, and CEC improvements.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and Morphological Characterization of the Entomopathogenic Nematode <i>Oscheius cyrus</i> (Nematoda: Rhabditidae) and Molecular Variability of <i>Heterorhabditis bacteriophora</i> from Georgia (Caucasus).","authors":"Oleg Gorgadze, Elena Fanelli, Alessio Vovlas, Alberto Troccoli, Eustachio Tarasco, Francesca De Luca","doi":"10.3390/biology14050512","DOIUrl":"10.3390/biology14050512","url":null,"abstract":"<p><p>Surveys were conducted in the village of Shamgona in the Samegrelo Region of West Georgia, and two nematode species belonging to the <i>Oscheius</i> genus and a population of <i>Heterorhabditis bacteriophora</i> were isolated. Integrative diagnosis, combining morphology, PCA, sequencing and phylogeny, was carried for <i>Oscheius</i> sp. from the soil of hazelnut orchard. This population shows high similarity at morphological and morphometrical levels with <i>O. cyrus</i> from Iran, and the main distinctive features with <i>O. cyrus</i> from Iran are the presence of eight lateral lines and males. Sequence and phylogenetic analyses by using ITS, D2-D3 expansion domains of the 28S rRNA gene, the partial 18S rRNA gene, and the mitochondrial COI confirmed the identification as <i>O. cyrus.</i> A second population of <i>Oscheius</i> sp. and <i>H. bacteriophora</i> was characterized only at the molecular and phylogenetic level. The phylogenetic analyses, based on ITS and D2-D3 expansion domains, revealed the occurrence of <i>O. insectivorus</i> in Georgia grouping with <i>O. insectivorus</i> sequences with high support. The phylogenetic trees of <i>H. bacteriophora</i>, based on D2-D3 expansion domains, ITS, the partial 18S rRNA gene, and mitochondrial COI confirmed the high variability among geographical populations. The present study reports on the occurrence of two species of <i>Oscheius</i> in Georgia for the first time.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Identification of a Glucuronyltransferase-Related Gene, <i>GlcAT-S,</i> with Putative Mucus Protection and Anti-Inflammatory Effects from Gut-Damaged <i>Drosophila</i> by Dextran Sulfate Sodium (DSS).","authors":"Seung Hun Lee, Dooseon Hwang, Jang-Won Lee, Tae-Won Goo, Eun-Young Yun","doi":"10.3390/biology14050513","DOIUrl":"10.3390/biology14050513","url":null,"abstract":"<p><p>The intestinal epithelium, which is protected by mucosal surfaces composed of mucins and other glycoproteins, functions as a selective barrier that absorbs nutrients while preventing the translocation of harmful substances. To understand the mechanisms between mucosal disruption and tissue inflammation, we orally administrated a mucus-disrupting agent, dextran sodium sulfate, to <i>Drosophila melanogaster</i> and screened 63 differentially expressed genes (DEGs). Through a database search using bioinformatics tools (CHEA3 and WebGestalt), we identified ELK1 as a potential key transcription factor for the selected DEGs, and among the 63 DEGs, ELK1-related genes, B3GAT3, FIBP, and TENT2 (<i>GlcAT-S</i>, <i>Fibp</i>, and <i>Wisp</i> in <i>Drosophila</i>), were selected as the relevant genes that respond to mucus disruption. We confirmed that enterocyte (EC)-specific <i>GlcAT-S</i> knockdown by RNAi significantly reduced gut length and increased intestinal stem cell proliferation in <i>Drosophila</i>. Additionally, in EC-specific <i>GlcAT-S</i>-knockdown flies, it was observed that the mucus-production-related genes, <i>Muc68D</i> and <i>Mur29B</i>, were specifically reduced, whereas the inflammatory cytokines <i>egr</i> and <i>upd3</i> were overexpressed. This study provides evidence that <i>GlcAT-S</i> is involved in the regulation of intestinal inflammation in <i>Drosophila</i> and plays a protective role against mucus disruption. Our findings suggest that <i>GlcAT-S</i> may be a potential therapeutic target for the treatment of intestinal inflammatory diseases such as IBD.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Thioredoxin Reductase Activity and Oxidation of Cellular Thiols by Antimicrobial Agent, 2-Bromo-2-nitro-1,3-propanediol, Causes Oxidative Stress and Cell Death in Cultured Noncancer and Cancer Cells.","authors":"Chao Jiang, Gary Krzyzanowski, Dinesh S Chandel, Wesley A Tom, Nirmalee Fernando, Appolinaire Olou, M Rohan Fernando","doi":"10.3390/biology14050509","DOIUrl":"10.3390/biology14050509","url":null,"abstract":"<p><strong>Background: </strong>The thioredoxin system (TrxS) is crucial for maintaining redox balance by regulating cellular thiol levels and is involved in various biological processes, including cancer progression. Thioredoxin reductase (TrxR), a key component of TrxS, reduces oxidized thioredoxin (Trx) using NADPH. This study investigates the inhibitory effects of 2-bromo-2-nitro-1,3-propanediol (Bronopol, BP), a preservative, on TrxR activity and its impact on cellular thiols and cell viability.</p><p><strong>Methods: </strong>Purified recombinant TrxR and noncancer and cancer cells were treated with different concentrations of BP and TrxR activity measured. BP-treated cells were examined for effects of BP on total cellular thiol level and GSH/GSSG ratio.</p><p><strong>Results: </strong>BP effectively inhibited TrxR in a dose-dependent manner, an effect that was reversible with dithiothreitol (DTT). BP treatment significantly reduced total thiol levels, decreased the GSH/GSSG ratio, and increased reactive oxygen species (ROS) in cells. Additionally, BP decreased cell viability and induced apoptosis, as indicated by morphological changes and increased c-fos mRNA expression.</p><p><strong>Conclusions: </strong>These findings highlight BP's potential as a TrxR inhibitor and its cytotoxicity toward both noncancer and cancer cells. The observed effects-TrxR inhibition, thiol oxidation, GSH/GSSG imbalance, and ROS accumulation-may underlie BP's cytotoxicity. Further research is needed to explore the precise molecular mechanisms by which BP exerts these effects.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Cytokine Expression Profiles in Prostate Cancer Patients.","authors":"Karoline Brito Caetano Andrade Coelho, Denise Kusma Wosniaki, Jonatas Luiz Pereira, Murilo Luz, Letusa Albrecht, Jeanine Marie Nardin, Mateus Nobrega Aoki, Leonardo O Reis, Rodolfo Borges Dos Reis, Dalila Lucíola Zanette","doi":"10.3390/biology14050505","DOIUrl":"10.3390/biology14050505","url":null,"abstract":"<p><p>This study aimed to identify the cytokine expression profile in prostate cancer (PCa) patients compared to healthy individuals. Plasma samples from 75 PCa patients and 14 healthy controls were analyzed using Multiplex ELISA (Luminex) to measure the expression levels of 12 cytokines: IL-4, IL-5, IL-6, IL-10, IL-1β, IL-17A, IL-12p70, MCP-1/CCL2, MIP-1α/CCL3, MIP-1β/CCL4, TNF-α, and IFN-γ. Differences in cytokine expression levels were analyzed using the Mann-Whitney test, Wilcoxon's rank-sum test, Spearman's rank correlation, and K-means Clustering unsupervised machine learning to validate cytokine expression patterns. In PCa patients, MIP-1α/CCL3, MIP-1β/CCL4, IFN-γ, and interleukins exhibited significantly higher expression levels; conversely, TNF-α and MCP-1/CCL2 both had decreased expression compared to healthy individuals. The clustering analysis confirmed that PCa patients exclusively exhibit the highest associations with MIP-1α/CCL3, IFN- γ, IL-12p70, IL-4, and IL-5. Furthermore, specific correlations between IL-4 and MIP-1 beta, IL-4 and IFN-gamma, IL-5 and IL-12p70, and IL-5 and IFN-gamma in PCa patients did not occur in healthy individuals. Such results will guide forthcoming in vitro and in vivo human prostate cancer-drug treatment models, paving the way for exploration of future drug targets and candidates with potential to predict FDA-approved prostate cancer treatment responses by targeting cytokine levels and the oncogenesis pathways.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radon Exposure and Cancer Risk: Assessing Genetic and Protein Markers in Affected Populations.","authors":"Yerlan Kashkinbayev, Baglan Kazhiyakhmetova, Nursulu Altaeva, Meirat Bakhtin, Pavel Tarlykov, Elena Saifulina, Moldir Aumalikova, Danara Ibrayeva, Aidos Bolatov","doi":"10.3390/biology14050506","DOIUrl":"10.3390/biology14050506","url":null,"abstract":"<p><p>Radon is an inert gas produced by the radioactive decay of uranium-238, commonly found in the environment. Radon and its decay products are the main sources of human exposure to radiation from natural sources. When inhaled, radon's alpha particles impact lung tissue, potentially causing lung cancer by damaging DNA and altering oxidative processes. This review article addresses the need for a deeper understanding of the genetic and molecular changes associated with radon-induced lung cancer, aiming to clarify key genetic mutations and protein markers linked to carcinogenesis. Particular attention in recent studies has been given to mutations in tumor suppressor genes (RASSF1, TP53), oncogenes (KRAS, EGFR), and changes in the expression levels of protein biomarkers associated with inflammation, stress, and apoptosis. Identifying these markers is critical for developing effective screening methods for radon-induced lung cancer, enabling timely identification of high-risk patients and supporting effective preventive strategies. Summarizing current genetic and protein biomarkers, this review highlights the importance of a comprehensive approach to studying radon-induced carcinogenesis. Understanding these molecular mechanisms could ultimately improve early diagnostic methods and enhance therapy for cancers associated with radon exposure.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Identification and Cold Stress Response Mechanism of Barley Di19 Gene Family.","authors":"Wenbo Chai, Chao Yuan, Shufen Li, Hanyuan Xu, Qing Zhu, Hongtao Li, Wei Ji, Jun Wang","doi":"10.3390/biology14050508","DOIUrl":"10.3390/biology14050508","url":null,"abstract":"<p><p>The Di19 (Drought-induced 19) gene family encodes Cys2/His2-type zinc finger proteins that are known to be involved in plant responses to various abiotic stresses, including drought, salinity, and temperature extremes. However, little is known about their roles in barley (<i>Hordeum vulgare</i>), particularly in cold stress adaptation. This study aimed to conduct a comprehensive genome-wide analysis of the barley genome to identify Di19 gene family members and examine their expression patterns under cold stress, providing theoretical support for stress-resistant barley breeding. By aligning Di19 gene sequences from <i>Arabidopsis</i> and rice and using BLASTp, seven <i>HvDi19</i> genes were identified in barley. Bioinformatics analysis revealed that all members contain a conserved Cys2/His2-type zinc finger domain and nuclear localization signals. Phylogenetic analysis grouped the <i>HvDi19</i> genes into four subfamilies, with three homologous gene pairs, and Ka/Ks analysis indicated strong purifying selection. Tissue-specific expression analysis showed significant variation in <i>HvDi19</i> expression across barley organs. Under cold stress, different barley varieties exhibited distinct <i>HvDi19</i> gene expression profiles: for instance, <i>HvDi19-1</i> was downregulated in cold-tolerant varieties, whereas <i>HvDi19-7</i> showed increased expression in a cold-tolerant mutant, suggesting their potential roles in modulating cold response. These findings reveal the evolutionary conservation and cold-responsive expression characteristics of the <i>HvDi19</i> gene family, laying a foundation for future functional studies. The results also provide important molecular resources for the genetic improvement of cold tolerance in barley, contributing to the development of stress-resilient crop varieties under climate change.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Methionine Metabolism Reveals AMPK-SAMTOR Signaling as a Therapeutic Vulnerability in Prostate Cancer.","authors":"Serdar Arisan, Ayyuce Sever, Pinar Obakan-Yerlikaya, Elif Damla Arisan, Pinar Uysal-Onganer","doi":"10.3390/biology14050507","DOIUrl":"10.3390/biology14050507","url":null,"abstract":"<p><p>Prostate cancer (PCa) is not only one of the most diagnosed malignancies in men but also a leading cause of cancer-related mortality globally. PCa exhibits unique metabolic dependencies, particularly on lipids and glutamine, unlike many solid tumors, rather than glycolysis. Methionine metabolism plays a crucial role in these metabolic pathways, contributing to polyamine biosynthesis, DNA methylation, and cellular signaling processes. Here, we demonstrate that methionine deprivation induces selective vulnerability in AMPK-deficient PC3 PCa cells by disrupting SAMTOR-mTOR signaling and triggering oxidative stress, lipid depletion, and autophagic responses. Through functional and proteomic analyses, we show that SAMTOR directly interacts with p-AMPK and modulates cell fate under methionine-limited conditions. Our findings establish a mechanistic link between methionine sensing and metabolic stress signaling in PCa, offering a new avenue for targeted intervention.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Future Climate Change Increases the Risk of Suitable Habitats for the Invasive Macrophyte <i>Elodea nuttallii</i>.","authors":"Yuhan Qi, Yu Zhang, Jiali Xue, Zhen Zhang, Jingjing Cao, Nianwan Yang, Fanghao Wan, Xiaoqing Xian, Wanxue Liu","doi":"10.3390/biology14050504","DOIUrl":"10.3390/biology14050504","url":null,"abstract":"<p><p><i>Elodea nuttallii</i> is an ornamental macrophyte native to North America that has been introduced to Europe and Asia, and having been established, has had detrimental effects on local aquatic ecosystems. In this study, we developed an optimized MaxEnt model to predict the global potential habitat suitability for <i>E. nuttallii</i> under the influence of climate change. The model incorporated 20 relevant impact factors and occurrence record data for <i>E. nuttallii</i>. The results reveal that under current and future climate scenarios, potentially suitable habitats for <i>E. nuttallii</i> can be found on six assessed continents, mainly in Western Europe, western and eastern North America, southeastern Asia, southeastern Oceania, and scattered coastal areas in South America and Africa. Moreover, temperature and precipitation were identified as factors having significant effects on the distribution of <i>E. nuttallii</i>. In the future, the area of habitats potentially suitable for <i>E. nuttallii</i> is predicted to expand, particularly towards higher latitudes.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory Efficacy of <i>Arthrospira platensis</i> Extracts on Skin Pathogenic Bacteria and Skin Cancer Cells.","authors":"Ranchana Rungjiraphirat, Nitsanat Cheepchirasuk, Sureeporn Suriyaprom, Yingmanee Tragoolpua","doi":"10.3390/biology14050502","DOIUrl":"10.3390/biology14050502","url":null,"abstract":"<p><p><i>Arthrospira platensis</i> (spirulina) is a cyanobacterium containing various phytochemical compounds associated with various antioxidant, antimicrobial, antiviral, anticancer, anti-inflammatory, and immune-promoting properties. The efficacy of ethanolic and methanolic crude extracts of <i>A. platensis</i> regarding antibacterial, antioxidant, and anticancer effects was determined in this study. The ethanolic extract showed the highest antioxidant activity by 8.96 ± 0.84 mg gallic acid equivalent per gram of extract (mg GAE/g extract), 53.03 ± 4.21 mg trolox equivalent antioxidant capacity per gram of extract (mg TEAC/g extract), and 48.06 ± 0.78 mg TEAC/g extract as determined by DPPH, ABTS, FRAP assays, respectively. Moreover, the ethanolic extract showed the highest total phenolic and flavonoid compound contents by 38.79 ± 1.61 mg GAE/g extract and 27.50 ± 0.53 mg of quercetin equivalent per gram of extract (mg QE/g extract). Gallic acid and quercetin in the extracts were also determined by HPLC. The antibacterial activity was evaluated by agar well diffusion and broth dilution methods on skin pathogenic bacteria, including <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, methicillin-resistant <i>S. aureus</i> (MRSA), <i>Micrococcus luteus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Cutibacterium acnes</i>. The inhibition zone of <i>A. platensis</i> extracts ranges from 9.67 ± 0.58 to 12.50 ± 0.50 mm. In addition, MIC and MBC values ranged from 31.25 to 125 mg/mL. The inhibition of biofilm formation and biofilm eradication by <i>A. platensis</i> ethanolic extract was 87.18% and 99.77%, as determined by the crystal violet staining assay. Furthermore, the anticancer activity of <i>A. platensis</i> on A375 human melanoma cells was examined. The ethanolic and methanolic extracts induced DNA apoptosis through both intrinsic and extrinsic pathways by upregulating the expression of caspase-3, caspase-8, and caspase-9. These findings suggested that <i>A. platensis</i> demonstrated promising antioxidant, antibacterial, and anticancer activities, emphasizing its potential as a natural therapeutic agent for the management of pathological conditions.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}