Biology-Basel最新文献_第8页

Assessing Low-Mineralized Surface Water via Danio rerio Embryo Toxicity Test: A Case Study of Glacial Lakes in the Northern Tien Shan. 低矿化度地表水胚胎毒性试验研究——以天山北部冰湖为例。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-30 DOI: 10.3390/biology15191718

Mariya A Suvorova, Anna S Nizkorodova, Alina A Dmitriyeva

{"title":"Assessing Low-Mineralized Surface Water via Danio rerio Embryo Toxicity Test: A Case Study of Glacial Lakes in the Northern Tien Shan.","authors":"Mariya A Suvorova, Anna S Nizkorodova, Alina A Dmitriyeva","doi":"10.3390/biology15191718","DOIUrl":"10.3390/biology15191718","url":null,"abstract":"Bioassays are rarely used in ecotoxicological studies of high-altitude lakes located above 3000 m a.s.l., as ultra-low mineralization of water poses a major obstacle to toxicity assessment in biological objects. For the first time, we conducted a 96 h bioassay of surface water from supraglacial, proglacial and moraine-dammed lakes of two distinct mountain ranges of the Northern Tien Shan (average elevations of 3000-3600 m a.s.l.) using Danio rerio embryos. The study demonstrates the feasibility of using the D. rerio embryo toxicity test to survey the initially low-mineralized surface water of high-altitude lakes. For several Ile Alatau lakes, analysis of lethal and sublethal endpoints (including molecular biomarkers cyp1a, xpc, and dpolb gene expression) demonstrated a mild toxic effect, whereas physicochemical analysis failed to reveal contamination exceeding maximum permissible concentrations. In the Dzungarian Alatau, significant pollution with total petroleum hydrocarbons was detected in the lake cascade beneath the remote Tuyte Glacier. An altitude-dependent effect on zebrafish embryos was observed, ranging from moderate to no toxicity. The obtained results indicate that D. rerio embryo toxicity test might be applied to ecotoxicological studies of ultra-fresh water sources supplied by the melting mountain cryosphere, thus simplifying, reducing the cost of, and expanding the monitoring of remote high-mountain areas.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of Gastrointestinal Parasites in Fecal Samples from Free-Grazing Ducks in Northeastern Thailand. 泰国东北部野鸭粪便中胃肠道寄生虫的发生。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-30 DOI: 10.3390/biology15191719

Teputid Kuasit, Tongjit Thanchomnang, Jitsuda Kullawat, Sujittra Yahom, Siriporn Kuanamon, Wanchai Maleewong, Weerachai Saijuntha, Chairat Tantrawatpan

{"title":"Occurrence of Gastrointestinal Parasites in Fecal Samples from Free-Grazing Ducks in Northeastern Thailand.","authors":"Teputid Kuasit, Tongjit Thanchomnang, Jitsuda Kullawat, Sujittra Yahom, Siriporn Kuanamon, Wanchai Maleewong, Weerachai Saijuntha, Chairat Tantrawatpan","doi":"10.3390/biology15191719","DOIUrl":"10.3390/biology15191719","url":null,"abstract":"Intestinal parasitic infections remain a persistent concern in tropical agricultural systems, where close interactions among animals, humans, and the environment facilitate parasite transmission. Free-grazing ducks, commonly raised in rice-field ecosystems in Thailand, may be exposed to a wide range of intestinal parasites. This cross-sectional study examined 312 fecal samples collected from five selected free-grazing duck flocks in northeastern Thailand from October to November 2025. All fecal samples were examined using the modified formalin-ethyl acetate concentration technique. Parasites were identified based on egg and larval morphology. Positive parasite detections were found in 168 samples, yielding an overall percentage of positive fecal samples (%PFS) of 53.8%. The %PFS across the five provinces ranged from 42.5% to 63.5%. Among the positive fecal samples, the most frequently detected parasites were unidentified nematodes (38.1%), trichurid-like eggs (30.3%), strongyle-type eggs (25.0%), echinostome-like eggs (21.4%), and Ascaridia spp. (18.4%). Among the positive samples, small operculated trematode (SOT) and hymenolepidid-like eggs were detected in 3.0% and 1.2%, respectively; however, these findings may represent spurious passage. Single morphotype detection predominated, while co-detection of multiple morphotypes occurred less frequently. These findings document a diverse assemblage of intestinal parasite morphotypes in fecal samples from free-grazing ducks in northeastern Thailand, potentially reflecting exposure through aquatic and soil-associated routes.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic and Metabolomic Analyses Reveal That Fusarium sp. Isolate Is Associated with Growth Promotion in Lolium perenne L. 转录组学和代谢组学分析表明，镰刀菌对黑麦草生长有促进作用。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-30 DOI: 10.3390/biology15191720

Ganglian Liu, Jiahui Li, Yanwen Wang, Zhenghai Sun, Liping Li

{"title":"Transcriptomic and Metabolomic Analyses Reveal That Fusarium sp. Isolate Is Associated with Growth Promotion in Lolium perenne L.","authors":"Ganglian Liu, Jiahui Li, Yanwen Wang, Zhenghai Sun, Liping Li","doi":"10.3390/biology15191720","DOIUrl":"10.3390/biology15191720","url":null,"abstract":"Most previous studies have focused on the pathogenicity of Fusarium species towards crops. In this study, a Fusarium isolate was obtained from healthy Chamelaucium uncinatum plants; its ITS rDNA sequence showed 99% similarity to that of Fusarium napiforme. The fermentation preparation derived from this isolate promoted the growth and biomass accumulation of Lolium perenne L., a high-quality temperate forage grass; following seed treatment with the fermentation broth, the germination rate increased from 61.5% to 82%. In the pot experiment, in which the fermentation preparation was applied by root irrigation, plant height and root length of the host plants increased significantly by 20.0% and 30.6% (p < 0.05) and dry weight increased by 67.6% (p = 0.010), whereas fresh weight showed a non-significant increasing trend (+22.5%, p = 0.136); no disease symptoms were observed. Integrated transcriptomic and metabolomic analyses showed that exposure to the fermentation broth was associated with changes in genes and metabolites related to biomass accumulation. This study identified a candidate fungal isolate for the future development of microbial inoculants for forage crops.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Social Isolation Causes Behavioural Abnormalities and Reduces Synaptic Proteins in the Brains of Drosophila. 社会隔离导致果蝇大脑行为异常并减少突触蛋白。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-30 DOI: 10.3390/biology15191721

Gurlaz Kaur, Heba Mahmood, James Thompson-Dick, Anna Phan

{"title":"Social Isolation Causes Behavioural Abnormalities and Reduces Synaptic Proteins in the Brains of Drosophila.","authors":"Gurlaz Kaur, Heba Mahmood, James Thompson-Dick, Anna Phan","doi":"10.3390/biology15191721","DOIUrl":"10.3390/biology15191721","url":null,"abstract":"Social isolation is known to cause a variety of behavioural abnormalities in many different species. Here we perform a detailed behavioural analysis of how chronic social deprivation alters behaviours in the fruit fly, Drosophila melanogaster, within an arena. We identify specific, quantifiable measures to capture behaviours of interest using the previously established cTrax and MATLAB BehavioralMicroarray toolkits. Socially isolated Drosophila exhibited behavioural changes in almost all measures assessed, identifying a surprisingly wide range of behavioural deficits. We observe that both male and female isolated flies demonstrate reduced locomotion (walk distance and speed), are more avoidant of other same-sex individuals (increased social distancing), and show reduced clustering into same-sex groups. Because these measures of sociality are assessed in same-sex groups, they are independent of sexual motivations. For the majority of measures taken, we observe the same behavioural changes in isolated male and female flies. In the brain, we observe that social isolation leads to a reduction in synaptic proteins specifically within dopamine neurons, as well as across the whole brain in both sexes, consistent with the varied behavioural abnormalities observed upon social isolation.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic Profiling Uncovers Physiological and Parasitological Adaptations to Bee Pollen Supplementation in Goats. 代谢组学分析揭示山羊对蜂花粉补充的生理和寄生适应性。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-29 DOI: 10.3390/biology15191717

Adriana Rojas, Lauren K Solice, Reagan N Sims, Brian L Bruner, Adeyemi A Olanrewaju, Rodolfo C Cardoso, Morteza Hosseini Ghaffari, Cesar A Rosales-Nieto

{"title":"Metabolomic Profiling Uncovers Physiological and Parasitological Adaptations to Bee Pollen Supplementation in Goats.","authors":"Adriana Rojas, Lauren K Solice, Reagan N Sims, Brian L Bruner, Adeyemi A Olanrewaju, Rodolfo C Cardoso, Morteza Hosseini Ghaffari, Cesar A Rosales-Nieto","doi":"10.3390/biology15191717","DOIUrl":"10.3390/biology15191717","url":null,"abstract":"Parasitic infections compromise livestock health, productivity, and profitability, while rising anthelmintic resistance restricts the effectiveness of conventional parasite control strategies. Bee pollen (BP) contains bioactive compounds with immunomodulatory and antimicrobial potential. This study investigated whether dietary BP supplementation reduces parasite burden and influences liver function, hematological and metabolic profiles, and circulating metabolomic signatures in goats. Thirty crossbred Boer × Spanish does were randomly assigned to either a BP-supplemented group (T-BP; n = 15) or a control group (T-CTL; n = 15). Over 49 days, T-BP goats received 10 g/day BP mixed with 50 g/day pelleted feed, while controls received the same feed without BP. Body weight, weekly blood samples, FAMACHA scores, and biweekly fecal egg counts (FEC) were collected. We assessed metabolomic profiles on days -8, 21, 42, and 56. Data were analyzed using mixed models and repeated measures. We identified strongyle-type eggs, presumably Haemonchus contortus, as the predominant parasite. No significant differences were observed between treatments for body weight, liver enzymes, metabolic biomarkers, or hematological profiles (p > 0.05). FEC varied over time (p < 0.001), with significantly lower FEC in BP-supplemented goats only on Day 42 (p < 0.05). Metabolomic differences were significant on Days 21 and 42 but not on Day 56. These differences involved metabolites annotated to lipid signaling, inflammatory processes, and cellular energy metabolism. In summary, BP supplementation demonstrated a potential antiparasitic effect against H. contortus but did not significantly affect liver, metabolic, or hematological parameters. Metabolomic findings should be considered exploratory and hypothesis-generating.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthetic Gene Cluster Mining and In Vitro Evaluation of the Antimicrobial and Cytotoxic Potential of Xenorhabdus thuongxuanensis. 生物合成基因簇挖掘及其体外抗菌和细胞毒性评价。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-28 DOI: 10.3390/biology15191716

Yaowapa Sikhaophong, Sophit Khanthawong, Jatuporn Ngoenkam, Sutthirat Sitthisak, Apichat Vitta, Liam K R Sharkey, Sacha J Pidot, Aunchalee Thanwisai

{"title":"Biosynthetic Gene Cluster Mining and In Vitro Evaluation of the Antimicrobial and Cytotoxic Potential of Xenorhabdus thuongxuanensis.","authors":"Yaowapa Sikhaophong, Sophit Khanthawong, Jatuporn Ngoenkam, Sutthirat Sitthisak, Apichat Vitta, Liam K R Sharkey, Sacha J Pidot, Aunchalee Thanwisai","doi":"10.3390/biology15191716","DOIUrl":"10.3390/biology15191716","url":null,"abstract":"Xenorhabdus species are prolific producers of diverse bioactive natural products. However, the genomic characteristics, biosynthetic potential, and biological activities of Xenorhabdus thuongxuanensis remain poorly characterized. This study aimed to characterize the genomic features and biosynthetic potential of X. thuongxuanensis strains KLK17.5 and KLK18.1 through comparative genomic analyses and to evaluate the antibacterial, antifungal, and cytotoxic activities of their crude extracts. Both strains had genome sizes and GC contents comparable to those of other Xenorhabdus species, with average nucleotide identity (ANI) values of 96-98% supporting their classification as X. thuongxuanensis. Functional annotation identified genes involved in secondary metabolism, while antiSMASH predicted diverse biosynthetic regions showing similarity to known BGCs, including those associated with holomycin, putrebactin, avaroferrin, and aryl polyenes. The crude extracts exhibited antibacterial activity against several antibiotic-resistant bacteria with minimum inhibitory concentration (MIC) values ranging from 0.2 to 6.25 mg/mL. They also inhibited Candida albicans and Cryptococcus neoformans, with inhibition zones ranging from 7.5 to 12 mm and from 10 to 42.5 mm, respectively. The crude extract from KLK18.1 exhibited cytotoxicity against HepG2 cells, with an IC50 value of 10.85 µg/mL. These findings expand the current knowledge of the genomic, biosynthetic, and biological potential of X. thuongxuanensis and provide a basis for future characterization of its BGC-associated metabolites.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systems-Level Transcriptomic and Cellular Architecture of the Human Striatum in Schizophrenia and Bipolar Psychosis. 精神分裂症和双相精神病患者纹状体的系统水平转录组学和细胞结构。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-26 DOI: 10.3390/biology15191714

Wei-Hung Chan, Shih-Ming Huang, Hui-Chen Su, Yi-Lin Chiu

{"title":"Systems-Level Transcriptomic and Cellular Architecture of the Human Striatum in Schizophrenia and Bipolar Psychosis.","authors":"Wei-Hung Chan, Shih-Ming Huang, Hui-Chen Su, Yi-Lin Chiu","doi":"10.3390/biology15191714","DOIUrl":"10.3390/biology15191714","url":null,"abstract":"Psychosis involves striatal circuit dysfunction, but the regional molecular and cellular changes remain unclear. We re-analyzed 215 postmortem RNA-seq samples (72 donors: 36 controls, 28 schizophrenia, 8 bipolar disorder with psychosis) from the nucleus accumbens, caudate and putamen, asking whether diagnosis-associated transcriptional changes are region-specific and whether they reflect cellular composition or cell-intrinsic state. Region-specific limma-voom models identified 0, 141 and 2152 schizophrenia-associated genes (FDR < 0.10) in the nucleus accumbens, caudate and putamen; the small bipolar-psychosis group yielded one gene, with limited power to detect additional associations. Correlation-aware camera tests supported immune-inflammatory Hallmark programs in the caudate and putamen, whereas nucleus accumbens signals were not robust to sequencing-run adjustment. Leukocyte-reference deconvolution was uninformative for brain parenchyma. Across four deconvolution estimators and two single-nucleus references, including an independent eight-donor striatal reference, putamen samples from donors with schizophrenia showed a consistently lower oligodendrocyte-lineage reference weight, whereas attribution to oligodendrocyte precursor cells specifically was method-dependent. Exploratory directional signatures (dopamine-adenosine, glutamate-calcium, Activin-SMAD, APP processing) remained supported in the caudate and putamen after direction-selection permutation conditional on fixed gene membership and were driven mainly by schizophrenia. The results characterize a dorsal-striatal, schizophrenia-associated transcriptional and oligodendroglial signal and provide prioritized hypotheses for independent validation.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophysiology of the Camel Heart: A Systematic Review of Evidence from Camelus dromedarius. 骆驼心脏的电生理：来自单峰骆驼证据的系统综述。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-26 DOI: 10.3390/biology15191711

Alyazia Alshamsi, Jawaher Abdulhakim, Mariam Alnuaimi, Azhar T Rahma, Reem AlSheryani, Ismail Elkonaisi, Frank C Howarth

{"title":"Electrophysiology of the Camel Heart: A Systematic Review of Evidence from Camelus dromedarius.","authors":"Alyazia Alshamsi, Jawaher Abdulhakim, Mariam Alnuaimi, Azhar T Rahma, Reem AlSheryani, Ismail Elkonaisi, Frank C Howarth","doi":"10.3390/biology15191711","DOIUrl":"10.3390/biology15191711","url":null,"abstract":"Objective: We aimed to synthesise the available evidence on dromedary-camel electrocardiographic (ECG) measurements, rhythm, and conduction while distinguishing direct electrophysiological studies from supporting cardiovascular reports. Methods: PubMed/MEDLINE, Scopus, Embase, Web of Science, Google Scholar, and supplementary sources were searched from inception to 22 December 2025. During revision, the research librarian verified that the saved strategies contained the principal ECG and conduction terms. This verification did not retrieve or screen additional database records. Results: Fifteen primary studies met the eligibility criteria, all involving Camelus dromedarius. Six provided direct ECG or electrical telemetry data, whereas nine provided supporting heart rate, Doppler, echocardiographic, or haemodynamic data that were not used to derive ECG intervals or arrhythmia frequency. Direct studies reported low resting heart rates, atrioventricular conduction times (PR intervals) of approximately 180-260 ms, and drug-associated rhythm or conduction abnormalities. Lead configuration, animal position, recording duration, sampling, and outcome reporting varied substantially. Biological sex was incompletely reported, and no study compared males with females. Conclusions: The directly measured ECG findings are preliminary descriptors rather than validated reference intervals. Confidence is limited by small samples, methodological heterogeneity, the dated search, and possible loss of baseline studies through the executed exposure/context block. The conclusions apply only to Camelus dromedarius. Registration: International Prospective Register of Systematic Reviews (PROSPERO), CRD420251169514.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Andrographolide Promotes Post-Ischemic Endothelial Angiogenic Activity via IL-10-Mediated M2-Like Microglia-Endothelial Crosstalk Involving Endothelial VEGFR2 Upregulation. 穿心莲内酯通过il -10介导的m2样小胶质细胞内皮串扰参与内皮细胞VEGFR2上调，促进缺血后内皮血管生成活性。

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-26 DOI: 10.3390/biology15191715

Hongfei Li, Hairong Xiang, Xinyu Li, Jiazhen Xie, Linfeng Zhao, Lan Yang, Riyi Shi, Yong Wang, Liangqin Shi

{"title":"Andrographolide Promotes Post-Ischemic Endothelial Angiogenic Activity via IL-10-Mediated M2-Like Microglia-Endothelial Crosstalk Involving Endothelial VEGFR2 Upregulation.","authors":"Hongfei Li, Hairong Xiang, Xinyu Li, Jiazhen Xie, Linfeng Zhao, Lan Yang, Riyi Shi, Yong Wang, Liangqin Shi","doi":"10.3390/biology15191715","DOIUrl":"10.3390/biology15191715","url":null,"abstract":"Ischemic stroke remains a leading cause of disability worldwide, and angiogenesis is critical for functional recovery. Although andrographolide (ANP) has anti-inflammatory and neuroprotective properties, its role in post-stroke angiogenesis remains unclear. Here, we investigated whether ANP promotes post-ischemic angiogenesis by modulating microglia-endothelial crosstalk. Using a ferric chloride (FeCl3)-induced carotid artery thrombosis model in male C57BL/6J mice, we found that ANP treatment (2.5 mg/kg) attenuated ischemic brain injury and neurological deficits, enhanced vascular stability, and improved cerebral blood perfusion. Mechanistically, ANP promoted a pro-reparative M2-like microglial state characterized by coordinated transcriptional changes, increased CD206 and IL-10, and reduced CD86 expression. ANP-treated microglia enhanced endothelial cell migration and tube formation while preserving tight-junction integrity through paracrine signaling. Co-immunoprecipitation and gain- and loss-of-function experiments further supported a microglial IL-10-dependent endothelial VEGFR2 upregulation. Together, these findings demonstrate that ANP promotes post-stroke reparative angiogenic activity through IL-10-mediated M2-like microglia-endothelial crosstalk involving endothelial VEGFR2 upregulation, linking its immunomodulatory effects to angiogenesis and highlighting microglia-endothelial crosstalk as a potential therapeutic target for ischemic stroke recovery.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage-Dependent Heat-Stress Mechanisms in Chinese Sturgeon (Acipenser sinensis) Early Life-Stages Under Simulated Thermal-Lag Effect. 模拟热滞后效应下中华鲟（Acipenser sinensis）早期阶段热应激机制的研究

IF 4.3 3区生物学

Biology-Basel Pub Date : 2026-09-26 DOI: 10.3390/biology15191713

Xiaoqian Leng, Junlin Yang, Xinchi Li, Qiong Zhou, Ke Zhang, Jiang Luo, Wei Xiong, Jinping Wu, Luoxin Li, Yuan Liu, Jinming Wu, Junyi Li

{"title":"Stage-Dependent Heat-Stress Mechanisms in Chinese Sturgeon (Acipenser sinensis) Early Life-Stages Under Simulated Thermal-Lag Effect.","authors":"Xiaoqian Leng, Junlin Yang, Xinchi Li, Qiong Zhou, Ke Zhang, Jiang Luo, Wei Xiong, Jinping Wu, Luoxin Li, Yuan Liu, Jinming Wu, Junyi Li","doi":"10.3390/biology15191713","DOIUrl":"10.3390/biology15191713","url":null,"abstract":"Hydraulic engineering-driven thermal lag disturbs natural water temperature and induces thermal stress in early-life-stage Chinese sturgeon (Acipenser sinensis), yet stage-specific molecular stress mechanisms remain unclear. This study assessed phenotypic, proteomic and metabolomic responses of endogenous-nutrition embryos and exogenous first-feeding larvae under 16 °C, 19 °C and 22 °C. Phenotypic results showed optimal embryo survival at 16 °C (hatching rate 64.6%), maximum larval growth at 19 °C (specific growth rate 6.79%/d), and severe developmental suppression at 22 °C (with the hatching rate dropping by 49.6 percentage points relative to 16 °C). Multi-omics uncovered distinct injury pathways: high temperature disrupted amino-acid and fatty-acid catabolism and triggered organic-acid accumulation and energy deficiency in embryos; in first-feeding larvae, it activated ferroptosis, polyunsaturated fatty-acid peroxidation and lysosomal pathways to produce oxidative damage. These findings demonstrate that a 1-3 °C temperature rise exceeds stage-specific thermal tolerance, with embryos succumbing to \"metabolic suppression-energy deficiency\" and larvae to \"oxidative stress-ferroptosis\". This study provides the first integrative proteomic and metabolomic evidence of stage-dependent thermal injury mechanisms in sturgeon, offering mechanistic insights critical for conservation physiology and informing conservation actions for this endangered sturgeon species.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"15 19","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13663467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149197438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0