Biology-Basel最新文献

{"title":"Genetic Variation and Gene Expression of the Antimicrobial Peptide Macins in Asian Buffalo Leech (<i>Hirudinaria manillensis</i>).","authors":"Yunfei Yu, Lizhou Tang, Mingkang Xiao, Jingjing Yin, Tianyu Ye, Rujiao Sun, Rui Ai, Fang Zhao, Zuhao Huang, Gonghua Lin","doi":"10.3390/biology14050517","DOIUrl":"10.3390/biology14050517","url":null,"abstract":"<p><p>With the growing severity of antibiotic resistance, antimicrobial peptides demonstrate significant potential for medical applications. Here, we performed genome and transcriptome sequencing of 30 Asian buffalo leech (<i>Hirudinaria manillensis</i>) individuals and integrated data from three other leech species (<i>Whitmania pigra</i>, <i>Hirudo nipponia</i>, and <i>Hirudo medicinalis</i>) to investigate genetic variation and gene expression of <i>H. manillensis</i> macins. Three macins (Hman1, Hman2, and Hman3), along with their encoding genes (<i>Hman1</i>, <i>Hman2</i>, and <i>Hman3</i>), were identified in <i>H. manillensis</i>. Hman1 exhibited the highest similarity (63.5 ± 12.0%) to macins from other leeches, followed by Hman2 (57.8 ± 7.4%) and Hman3 (30.0 ± 3.5%). Both amino acid and codon sequences of Hman1 were conserved within the species, whereas Hman2 and Hman3 exhibited markedly higher variability. All <i>Hman1</i> sequences were translatable, while four <i>Hman2</i> and 28 <i>Hman3</i> sequences had degenerated into pseudogenes. Transcripts per million (TPM) values for <i>Hman1</i>, <i>Hman2</i>, and <i>Hman3</i> were 2196.63, 242.35, and 1.22, respectively. Total macin expression in <i>H. manillensis</i> was less than 1/20 of that in <i>W. pigra</i>. Based on sequence variation and expression patterns, we propose that Hman1 retains functionality while Hman2 and Hman3 have lost or are losing their antibacterial functions.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Imaging Techniques for Mammalian/Human Ciliated Cell's Cilia: Insights into Structure, Function, and Dynamics.","authors":"Jin Li, Shiqin Huang, Hao Chen","doi":"10.3390/biology14050521","DOIUrl":"10.3390/biology14050521","url":null,"abstract":"<p><p>Cilia are evolutionarily conserved, microtubule-based organelles characterized by their ultrastructures and diverse functional roles, including developmental signaling, mechanosensation, and fluid propulsion. They are widely distributed across cell surfaces and play crucial roles in cell cycle regulation and tissue homeostasis. Despite advances in studying their molecular regulation and functions, demonstrating the precise ultrastructure of cilia remains a challenge. Recent novel microscopy techniques, such as super-resolution microscopy and volume electron microscopy, are revolutionizing our understanding of their architecture and mechanochemical signaling. By integrating findings from different methodologies, this review highlights how these advances bridge basic research and clinical applications and provide a comprehensive understanding of the structural organization, functional mechanisms, and dynamic changes of cilia.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the Impact of Ecological Restoration on Waterbird Diversity and Habitat Quality in Myanmar's Moe Yun Gyi Wetland.","authors":"Phyoe Marnn, Haider Ali, Haibo Jiang, Yang Liu, Ziqi Li, Sarfraz Ahmed, Tao Yang, Ziwei Li, Chunguang He","doi":"10.3390/biology14050519","DOIUrl":"10.3390/biology14050519","url":null,"abstract":"<p><p>The Moe Yun Gyi constructed wetland in Myanmar, located along the East Asian-Australian bird migration path, serves as a crucial habitat for migrating waterbirds. Protecting this biodiverse habitat is crucial in the face of ongoing threats, highlighting the need for effective conservation strategies. This study aims to evaluate the effects of the restoration on the populations of waterbirds from January 2014 to January 2024, by analyzing the composition and the spatiotemporal dynamics of waterbirds communities, with particular reference to changes in diversity. We measured waterbird quantity and species richness using the point count method, employing the Shannon-Wiener and D_G-F Index formulae for diversity analysis. Habitat analysis was conducted with ArcGIS 10.6 and eCognition 10.3, while data analysis utilized RStudio and Microsoft Excel. The results indicate that restoration efforts in 2016 initially led to a decline in waterbird populations. Restoration efforts after 2017 resulted in a twofold increase in both diversity and abundance. However, in 2023, flooding in Bago City caused a decline in waterbird populations, impacting habitat rehabilitation. There was a notable rise in uncommon waterbird populations, especially threatened <i>Pelecaniformes</i>. The community's richness has partially recovered, but seasonal water level volatility continues to impact diversity. The application of restoration techniques directly alters the extent and depth of water bodies, which are essential parameters for supporting appropriate waterbird habitats. We propose implementing a rational system for managing water levels, optimizing wetland hydrology, and enhancing water level regulation to safeguard significant resting areas along migration paths.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying Molecular Properties of Ataxin-2 Inhibitors for Spinocerebellar Ataxia Type 2 Utilizing High-Throughput Screening and Machine Learning.","authors":"Smita Sahay, Jingran Wen, Daniel R Scoles, Anton Simeonov, Thomas S Dexheimer, Ajit Jadhav, Stephen C Kales, Hongmao Sun, Stefan M Pulst, Julio C Facelli, David E Jones","doi":"10.3390/biology14050522","DOIUrl":"10.3390/biology14050522","url":null,"abstract":"<p><p>Spinocerebellar ataxia type 2 (SCA2) is an autosomal dominant neurodegenerative disorder marked by cerebellar dysfunction, ataxic gait, and progressive motor impairments. SCA2 is caused by the pathologic expansion of CAG repeats in the ataxin-2 (<i>ATXN2</i>) gene, leading to a toxic gain-of-function mutation of the ataxin-2 protein. Currently, SCA2 therapeutic efforts are expanding beyond symptomatic relief to include disease-modifying approaches such as antisense oligonucleotides (ASOs), high-throughput screening (HTS) for small molecule inhibitors, and gene therapy aimed at reducing <i>ATXN2</i> expression. In the present study, data mining and machine learning techniques were employed to analyze HTS data and identify robust molecular properties of potential inhibitors of <i>ATXN2</i>. Three HTS datasets were selected for analysis: <i>ATXN2</i> gene expression, CMV promoter expression, and biochemical control (luciferase) gene expression. Compounds displaying significant <i>ATXN2</i> inhibition with minimal impact on control assays were deciphered based on effectiveness (E) values (<i>n</i> = 1321). Molecular descriptors associated with these compounds were calculated using MarvinSketch (<i>n</i> = 82). The molecular descriptor data (MD model) was analyzed separately from the experimentally determined screening data (S model) as well as together (MD-S model). Compounds were clustered based on structural similarity independently for the three models using the SimpleKMeans algorithm into the optimal number of clusters (<i>n</i> = 26). For each model, the maximum response assay values were analyzed, and E values and total rank values were applied. The S clusters were further subclustered, and the molecular properties of compounds in the top candidate subcluster were compared to those from the bottom candidate subcluster. Six compounds with high <i>ATXN2</i> inhibiting potential and 16 molecular descriptors were identified as significantly unique to those compounds (<i>p</i> < 0.05). These results are consistent with a quantitative HTS study that identified and validated similar small-molecule compounds, like cardiac glycosides, that reduce endogenous ATXN2 in a dose-dependent manner. Overall, these findings demonstrate that the integration of HTS analysis with data mining and machine learning is a promising approach for discovering chemical properties of candidate drugs for SCA2.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Analysis Reveals the Molecular Features and Immune Infiltration of PANoptosis-Related Genes in Metabolic Dysfunction-Associated Steatotic Liver Disease.","authors":"Yan Huang, Jingyu Qian, Zhengyun Luan, Junling Han, Limin Tang","doi":"10.3390/biology14050518","DOIUrl":"10.3390/biology14050518","url":null,"abstract":"<p><strong>Background: </strong>Metabolic dysfunction-associated steatotic liver disease (MASLD), a chronic inflammatory disorder characterized by alcohol-independent hepatic lipid accumulation, remains poorly understood in terms of PANoptosis involvement.</p><p><strong>Methods: </strong>We integrated high-throughput sequencing data with bioinformatics to profile differentially expressed genes (DEGs) and immune infiltration patterns in MASLD, identifying PANoptosis-associated DEGs (PANoDEGs). Machine learning algorithms prioritized key PANoDEGs, while ROC curves assessed their diagnostic efficacy. Cellular, animal, and clinical validations confirmed target expression.</p><p><strong>Results: </strong>Three PANoDEGs (SNHG16, Caspase-6, and Dynamin-1-like protein) exhibited strong MASLD associations and diagnostic significance. Immune profiling revealed elevated M1 macrophages, naïve B cells, and activated natural killer cells in MASLD tissues versus controls. Further experiments verified the expression of the key PANoDEGs.</p><p><strong>Conclusions: </strong>This study provides new insights for further studies on the pathogenesis and treatment strategies of PANoptosis in MASLD.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Intelligence in Aquatic Biodiversity Research: A PRISMA-Based Systematic Review.","authors":"Tymoteusz Miller, Grzegorz Michoński, Irmina Durlik, Polina Kozlovska, Paweł Biczak","doi":"10.3390/biology14050520","DOIUrl":"10.3390/biology14050520","url":null,"abstract":"<p><p>Freshwater ecosystems are increasingly threatened by climate change and anthropogenic activities, necessitating innovative and scalable monitoring solutions. Artificial intelligence (AI) has emerged as a transformative tool in aquatic biodiversity research, enabling automated species identification, predictive habitat modeling, and conservation planning. This systematic review follows the PRISMA framework to analyze AI applications in freshwater biodiversity studies. Using a structured literature search across Scopus, Web of Science, and Google Scholar, we identified 312 relevant studies published between 2010 and 2024. This review categorizes AI applications into species identification, habitat assessment, ecological risk evaluation, and conservation strategies. A risk of bias assessment was conducted using QUADAS-2 and RoB 2 frameworks, highlighting methodological challenges, such as measurement bias and inconsistencies in the model validation. The citation trends demonstrate exponential growth in AI-driven biodiversity research, with leading contributions from China, the United States, and India. Despite the growing use of AI in this field, this review also reveals several persistent challenges, including limited data availability, regional imbalances, and concerns related to model generalizability and transparency. Our findings underscore AI's potential in revolutionizing biodiversity monitoring but also emphasize the need for standardized methodologies, improved data integration, and interdisciplinary collaboration to enhance ecological insights and conservation efforts.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACE- and DPP-IV-Inhibitory Peptides from Bambara Groundnut Hydrolysate: Elucidation Using Computational Tools and Molecular Docking.","authors":"Jirakrit Saetang, Thaiyawat Haewphet, Krisana Nilsuwan, Soottawat Benjakul","doi":"10.3390/biology14050511","DOIUrl":"10.3390/biology14050511","url":null,"abstract":"<p><p>Hypertension and type 2 diabetes are the major metabolic syndromes, often managed using synthetic ACE and DPP-IV inhibitors that may cause adverse effects on health. This study investigated Bambara groundnut protein hydrolysates as a natural source of dual ACE- and DPP-IV-inhibitory peptides. Protein isolates were hydrolyzed using Flavourzyme, and the resulting peptides were fractionated using membranes with different molecular weight cut-offs. Those fractions were then analyzed for enzyme inhibition. Peptides were identified by LC-MS/MS and screened using PeptideRanker and BIOPEP-UWM, followed by molecular docking against ACE (PDB: 1O8A) and DPP-IV (PDB: 1NU6). The >10 kDa and 5-10 kDa fractions showed the highest ACE- and DPP-IV-inhibitory activities, respectively. Some peptides such as YKDGLYSPHW, LPVSTPGKF, and EPWWPK displayed strong binding affinities (ΔG: -10.2 to -11.3 kcal/mol for ACE, -8.6 to -9.1 kcal/mol for DPP-IV) and interacted with key catalytic residues, including His387 and Glu411 in ACE, and Ser630, Glu205, and Phe357 in DPP-IV. These findings highlight the potential of Bambara groundnut hydrolysates or peptides as a source of natural ACE and DPP-IV inhibitors.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth Process and Mortality of <i>Sasa borealis</i> Seedlings over Six Years Following Mass Flowering and Factors Influencing Them.","authors":"Hanami Suzuki, Hisashi Kajimura","doi":"10.3390/biology14050516","DOIUrl":"10.3390/biology14050516","url":null,"abstract":"<p><p>The sexual reproduction of <i>Sasa borealis</i>, a species of dwarf bamboo, occurred in central Japan from 2016 to 2017. <i>S. borealis</i> grows on the forest floor and serves as an important source of habitat and food for various animals. Sexual reproduction occurs in synchrony among individuals in a given area, leading to a decline in population and causing substantial disturbances to the forest ecosystem; however, the subsequent regeneration process remains unclear. In this study, we investigated <i>S. borealis</i> seedling regeneration over six years. Fixed plots were established in the forest in the year following the sexual reproductive event, and the growth of seedlings was monitored from seed emergence to seedling growth at the individual level. We considered biotic and abiotic factors to evaluate their influence on regeneration. We examined mammalian and arthropod foraging as biotic factors. Conversely, abiotic factors included temperature and humidity near the ground surface, solar radiation, soil conditions, and snow cover. Seedling growth was characterized by a slow rate and affected by morphological changes resulting from foraging and abiotic factors. The return of <i>S. borealis</i> to its presexual reproductive stage requires an extended duration. Our study provides precious information for future <i>S. borealis</i> conservation and management strategies.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Status and Best Management Practices of Potato Early Dying Disease in New Brunswick, Canada.","authors":"Khalil I Al-Mughrabi, Rene Poirier, Salah Eddin Khabbaz","doi":"10.3390/biology14050514","DOIUrl":"10.3390/biology14050514","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Potato early dying (PED) disease complex is often called the &lt;i&gt;Verticillium&lt;/i&gt; wilt of potato and is considered one of the most economically devastating diseases of potato worldwide. The severity of the disease greatly increases with the association of the soil-borne pathogens &lt;i&gt;Verticillium dahliae&lt;/i&gt; and &lt;i&gt;V. albo-atrum&lt;/i&gt; and the root lesion nematode (&lt;i&gt;Pratylenchus&lt;/i&gt; sp.). Recently, an increase in wilt disease symptoms and a sharp decline in marketable tuber yield were observed in New Brunswick (NB), Canada. A survey of 71 fields, along with eight fumigated and eight non-fumigated fields, was carried out to determine and quantify nematodes and &lt;i&gt;Verticillium&lt;/i&gt; in the soil. Techniques used included plate counts for &lt;i&gt;Verticillium&lt;/i&gt; (CFU/g soil), real-time qPCR (RT-qPCR) for &lt;i&gt;V. dahliae&lt;/i&gt; (cell/g soil), and nematode identification and counts (# of nematodes/kg of soil). The survey results of the 71 fields revealed that 55 fields had &lt;i&gt;Verticillium&lt;/i&gt; sp. ranging from 2 to 66 CFU/g of soil by the plate method, and 68 fields had high &lt;i&gt;V. dahliae&lt;/i&gt; ranging from 261 to 27,471 cell/g of soil by RT-qPCR method. All fields had high numbers of root lesion nematodes ranging from 560 to 14,240 nematodes/kg of soil. There was an uneven distribution of PED incidence in potato fields at various locations of NB. Fumigation with Chloropicrin significantly reduced the numbers of root lesion nematodes by 34.1-99.0%, &lt;i&gt;Verticillium&lt;/i&gt; sp. CFU/g of soil by 50-100%, and &lt;i&gt;V. dahliae&lt;/i&gt; cell/g soil by 38-91% in the eight fumigated fields. The management of the PED complex with various disease management products under field conditions was also studied in a field plot trial setup. The nematicide Velum applied in-furrow at the recommended label rate decreased the numbers of root lesion nematodes by up to 66% compared to other products. The combination of both Velum + Aprovia and the application of ammonium-lignosulfonate significantly reduced &lt;i&gt;V. dahliae&lt;/i&gt; by 190.55% and 274.24%, respectively, compared to other products. The fungicide Aprovia applied in-furrow at the recommended rate for the management of &lt;i&gt;Verticillium&lt;/i&gt; wilt significantly reduced &lt;i&gt;Verticillium&lt;/i&gt; sp. CFU/g of soil in treated soil by 73.3% compared to Velum, Mustgrow, Senator PSPT, Vapam, ammonium-lignosulfonate, Nimitz, and the untreated control. Disease management products increased potato marketable yield by 27.38-97.74%. The results of this study suggest that the root lesion nematode and &lt;i&gt;V. dahliae&lt;/i&gt; have a ubiquitous distribution in the fields cultivated with potatoes in NB. The co-infection of potato by both &lt;i&gt;V. dahliae&lt;/i&gt; and the root lesion nematode can greatly increase the severity of PED. Fumigation with Chloropicrin significantly reduced the levels of root lesion nematodes and &lt;i&gt;Verticillium&lt;/i&gt; in all fumigated fields. Management practices of PED using the fungicide Aprovia, the nematicide Velum, and a combination of both Velum + Apro","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12109294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and Spatial Population Genetic Variation in Chilean Jack Mackerel (<i>Trachurus murphyi</i>).","authors":"Cristian B Canales-Aguirre, Sandra Ferrada Fuentes, Ricardo Galleguillos","doi":"10.3390/biology14050510","DOIUrl":"10.3390/biology14050510","url":null,"abstract":"<p><p><i>Trachurus murphyi</i> have been studied for population genetic structures for decades, identifying only one large population across the South Pacific Ocean. Although all of these studies have extensively examined the spatial genetic pattern, there remains a gap in understanding the potential role of temporality. Our study aims to elucidate spatial and temporal genetic patterns in <i>T. murphyi</i> populations in the South Pacific Ocean, examining genetic composition across seasons, including feeding and spawning seasons, where the latter was not previously investigated. Using 10 microsatellite loci, our study confirms an overall consistent and stable population genetic pattern in <i>T. murphyi</i> across its geographic distribution observed over multiple years and seasons. The only exception was found for New Zealand in the spring-summer season. Furthermore, we identify potential genetic markers for monitoring variability in the species.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 5","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12108880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}