Biology-Basel最新文献

Functional and Genomic Evidence of L-Arginine-Dependent Bacterial Nitric Oxide Synthase Activity in Paenibacillus nitricinens sp. nov. 硝化芽孢杆菌l -精氨酸依赖性细菌一氧化氮合酶活性的功能和基因组证据

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060733

Diego Saavedra-Tralma, Alexis Gaete, Carolina Merino-Guzmán, Maribel Parada-Ibáñez, Francisco Nájera-de Ferrari, Ignacio Jofré-Fernández

{"title":"Functional and Genomic Evidence of L-Arginine-Dependent Bacterial Nitric Oxide Synthase Activity in Paenibacillus nitricinens sp. nov.","authors":"Diego Saavedra-Tralma, Alexis Gaete, Carolina Merino-Guzmán, Maribel Parada-Ibáñez, Francisco Nájera-de Ferrari, Ignacio Jofré-Fernández","doi":"10.3390/biology14060733","DOIUrl":"10.3390/biology14060733","url":null,"abstract":"Although nitric oxide (NO) production in bacteria has traditionally been associated with denitrification or stress responses in model or symbiotic organisms, functionally validated L-arginine-dependent nitric oxide synthase (bNOS) activity has not been documented in free-living, non-denitrifying soil bacteria. This paper reports Paenibacillus nitricinens sp. nov., a bacterium isolated from rainforest soil capable of synthesizing NO via a bNOS under aerobic conditions. A bnos-specific PCR confirmed gene presence, while whole-genome sequencing (6.7 Mb, 43.79% GC) revealed two nitrogen metabolism pathways, including a bnos-like gene. dDDH (<70%) and ANI (<95%) values with related Paenibacillus strains support the delineation of this isolate as a distinct species. Extracellular and intracellular NO measurements under aerobic conditions showed a dose-dependent response, with detectable production at 0.1 µM L-arginine and saturation at 100 µM. The addition of L-NAME reduced NO formation, confirming enzymatic mediation. The genomic identification of a bnos-like gene strongly supports the presence of a functional pathway. The absence of canonical nitric oxide reductase (Nor) genes or other typical denitrification-related enzymes reinforces that NO production arises from an alternative, intracellular enzymatic mechanism rather than classical denitrification. Consequently, P. nitricinens expands the known repertoire of microbial NO synthesis and suggests a previously overlooked source of NO flux in well-aerated soils.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic and Biochemical Responses of Juvenile Babylonia areolata to Hypoxia Stress. 雏鸟对缺氧胁迫的代谢和生化反应。

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060727

Baojun Tang, Xiaoyao Ren, Zhiguo Dong, Hanfeng Zheng, Yujia Liu, Tao Wei

{"title":"Metabolic and Biochemical Responses of Juvenile Babylonia areolata to Hypoxia Stress.","authors":"Baojun Tang, Xiaoyao Ren, Zhiguo Dong, Hanfeng Zheng, Yujia Liu, Tao Wei","doi":"10.3390/biology14060727","DOIUrl":"10.3390/biology14060727","url":null,"abstract":"As an important aquaculture species, the marine snail Babylonia areolata is frequently subjected to fluctuation in dissolved oxygen concentration during farming and transportation processes. In the present study, we investigated the metabolic rates, transcription, and enzyme level responses of juvenile B. areolata exposed to long-term stress (144 h). The results showed that the mortality rate of juvenile B. areolata was higher in the 0.5 mg O2/L group compared to the 2 mg O2/L group. During the hypoxic stress period, both oxygen consumption and ammonia excretion rates were observed to be lower in juvenile B. areolata than those in the control group. As hypoxic stress duration prolonged, juvenile B. areolata demonstrated significantly elevated activities of pyruvate kinase (PK) and alkaline phosphatase (AKP), alongside reduced activities of lactic dehydrogenase (LDH), acid phosphatase (ACP), and superoxide dismutase (SOD). Significant changes in the expression levels of PK and LDH genes were observed during the hypoxic stress. The expression levels of ACP and SOD genes were significantly downregulated, while juvenile B. areolata exhibited elevated AKP gene expression levels under 0.5 mg O2/L. Our findings suggest that under long-term exposure to hypoxia, B. areolata failed to maintain energy homeostasis and suffered biochemical disruptions, leading to a reduction in survival. The mortality rate of B. areolata can be substantially decreased by ensuring that transportation time does not exceed 48 h.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Degradation of Aflatoxin B1 by Myroides odoratimimus 3J2MO. 芳香密菇3J2MO降解黄曲霉毒素B1的研究

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060724

Xue Wang, Yao-Yao Gao, Dun Wang, Qi Zhang, Hao-Ran Wang, Ting-Ting Zhang, Meng-Jie Zhu, Jing Dong, Dong Ling, Peng Feng, Xue-Hui Tang, Pei-Wu Li

{"title":"Study on the Degradation of Aflatoxin B1 by Myroides odoratimimus 3J2MO.","authors":"Xue Wang, Yao-Yao Gao, Dun Wang, Qi Zhang, Hao-Ran Wang, Ting-Ting Zhang, Meng-Jie Zhu, Jing Dong, Dong Ling, Peng Feng, Xue-Hui Tang, Pei-Wu Li","doi":"10.3390/biology14060724","DOIUrl":"10.3390/biology14060724","url":null,"abstract":"To address the issue of aflatoxin contamination, which poses a significant threat to food safety and human health, we have conducted extensive research. We have isolated a strain of Myroides odoratimimus (3J2MO) from the soil that exhibited remarkable efficiency in degrading various aflatoxin types, including AFB1, AFB2, AFG1, AFG2, and AFM1. SDS-PAGE analysis confirmed the purity of the enzymes to be over 95%. Through fluorescence assays, we quantified the enzymatic activity, with an AFB1 degradation rate of 95% achieved at 37 °C and a pH of 8.0. Further analysis using HPLC-MS/MS identified the degradation intermediates, revealing the mechanisms of lactone ring cleavage and epoxy group hydrolysis. GO/COG/KEGG annotations provided insights into the functions of these enzymes, with peroxidase linked to reactive oxygen species (ROS) generation and helicase associated with ATP-dependent conformational changes. Helicase, on the other hand, hydrolyzes ATP, driving conformational changes in AFB1 and facilitating its breakdown into non-toxic metabolites. The potential industrial-scale application of this discovery could significantly mitigate aflatoxin-related economic losses while minimizing chemical residues in the food chain.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Specimen Preservation Using DESS and DNA Extraction in Museum Collections. 利用DESS和DNA提取技术保存博物馆藏品中的DNA标本。

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060730

Eri Ogiso-Tanaka, Daisuke Shimada, Akito Ogawa, Genki Ishiyama, Ken-Ichi Okumura, Kentaro Hosaka, Chikako Ishii, Kyung-Ok Nam, Masakazu Hoshino, Shuhei Nomura, Showtaro Kakizoe, Yasuhide Nakamura, Isao Nishiumi, Minako Abe Ito, Taiju Kitayama, Norio Tanaka, Tsuyoshi Hosoya, Utsugi Jinbo

{"title":"DNA Specimen Preservation Using DESS and DNA Extraction in Museum Collections.","authors":"Eri Ogiso-Tanaka, Daisuke Shimada, Akito Ogawa, Genki Ishiyama, Ken-Ichi Okumura, Kentaro Hosaka, Chikako Ishii, Kyung-Ok Nam, Masakazu Hoshino, Shuhei Nomura, Showtaro Kakizoe, Yasuhide Nakamura, Isao Nishiumi, Minako Abe Ito, Taiju Kitayama, Norio Tanaka, Tsuyoshi Hosoya, Utsugi Jinbo","doi":"10.3390/biology14060730","DOIUrl":"10.3390/biology14060730","url":null,"abstract":"Recent advances in DNA research have increased the necessity for museums to preserve not only morphological specimens but also their DNA, leading us to maintain tissue samples linked to specimens at -80 °C. DNA analysis has become an essential tool for taxonomic research and biodiversity assessment; however, freezer storage for all samples is impractical due to space limitations and operational costs. This creates a pressing need to develop more widely applicable DNA preservation methods. We investigated the comparative effects of traditional preservation methods versus DESS (DMSO/EDTA/saturated NaCl solution) preservation on both morphology and DNA integrity using museum specimens from various taxonomic groups. Our results demonstrated that DESS preservation maintained high-quality DNA fragments exceeding >15 kb at room temperature across all examined species, with nematode samples maintaining DNA integrity even after 10 years of storage. When preserving whole organisms, the optimal preservation solution conditions for maintaining both morphological features and DNA integrity varied among species. Notably, DNA integrity was maintained even after complete evaporation of the DESS solution. These findings suggest that DESS utilization for specimen DNA preservation is effective across many species, not only for long-term storage in environments without freezer facilities but also for temporary preservation until freezing.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Zooplankton Community Assembly and Their Associations with Environmental Drivers in Arid-Region Reservoirs of Northwest China. 西北干旱区水库浮游动物群落聚集机制及其与环境驱动因素的关系

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060732

Xuelian Qiu, Fangze Zi, Long Yun, Qiang Huo, Liting Yang, Yong Song, Shengao Chen

{"title":"Mechanisms of Zooplankton Community Assembly and Their Associations with Environmental Drivers in Arid-Region Reservoirs of Northwest China.","authors":"Xuelian Qiu, Fangze Zi, Long Yun, Qiang Huo, Liting Yang, Yong Song, Shengao Chen","doi":"10.3390/biology14060732","DOIUrl":"10.3390/biology14060732","url":null,"abstract":"This study investigates the mechanisms of zooplankton community assembly and their relationship to environmental factors in high-latitude arid regions. We conducted seasonal sampling at four reservoirs in the upper Tarim River Basin from 2023 to 2024: Shangyou Reservoir (SY), Shengli Reservoir (SL), Duolang Reservoir (DL) and Xinjingzi Reservoir (XJZ). The zooplankton community was categorized into five functional groups based on the predominant species, with small crustacean filter feeders (SCF) in all reservoirs except XJZ, where a seasonal shift between rotifer collectors (RC) in the wet season and SCF in the dry season was observed. Pearson correlation and canonical correspondence analysis (CCA) revealed that interspecific competition, pH, conductivity (COND), and salinity (SALIN) were the main determinants of zooplankton community composition. Significant correlations (p < 0.05) were detected among functional groups RC (rotifers carnivora), RF (rotifers filter feeders), SCF (small copepods and claocera filter feeders), and MCC (middle copepods and claocera carnivora). Environmental factors showed significant spatial heterogeneity, while zooplankton biomass was positively correlated with pH and COND. Cluster similarity analyses indicated complex interactions between 29 zooplankton species, with RF identified as an important positive predictor for larger groups. The network of co-occurrences showed predominantly positive relationships, emphasizing the mutual facilitation between the species. Our results suggest that interspecific interactions have stronger effects on community structuring than environmental factors, with mutual facilitation emerging as an important survival strategy. This study provides important insights into the dynamics of zooplankton communities in dry reservoirs and establishes a framework for understanding ecological patterns and assembly mechanisms under drought conditions.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Aletris spicata (Thunb.) Franch. Extract in Asthma Therapy: Oxidative Stress, Inflammation, and Gut Microbiota. 刺痛的机制（拇指）法兰治（姓氏）。哮喘治疗中的提取物：氧化应激、炎症和肠道微生物群。

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060731

Jing Yang, Zhiyong Chen, Yue Zhu, Teng Chen, Ying Zhou, Zuhua Wang

{"title":"Mechanisms of Aletris spicata (Thunb.) Franch. Extract in Asthma Therapy: Oxidative Stress, Inflammation, and Gut Microbiota.","authors":"Jing Yang, Zhiyong Chen, Yue Zhu, Teng Chen, Ying Zhou, Zuhua Wang","doi":"10.3390/biology14060731","DOIUrl":"10.3390/biology14060731","url":null,"abstract":"Aletris spicata (Thunb.) Franch. (AS), a traditional edible and medicinal plant for treating asthma, was investigated for its therapeutic mechanisms. Liquid chromatography-high-resolution tandem mass spectrometry (LC-HRMS/MS) analysis identified 33 compounds in AS. In ovalbumin (OVA)-induced asthmatic mice, AS significantly reduced inflammatory cells (neutrophils, lymphocytes, eosinophils) in bronchoalveolar lavage fluid (BALF) and decreased IL-4, IL-5, IL-13, TNF-α, and serum IgE while increasing IFN-γ. AS alleviated lung and intestinal inflammation, reduced ROS and MDA levels, and enhanced SOD activity. Immunohistochemistry and Western blot revealed AS upregulated Nrf2/HO-1 expression and inhibited NF-κB p65 nuclear translocation. Gut microbiota studies demonstrated AS restored intestinal flora homeostasis by modulating the richness, diversity, and composition. Spearman correlation analysis identified significant relationships between oxidative stress markers, inflammatory cytokines, and specific gut bacteria. These findings indicate that AS mitigates asthma through antioxidant effects (Nrf2/HO-1 pathway), anti-inflammatory actions (NF-κB pathway), and gut microbiota modulation. The study provides a scientific basis for developing AS as a natural anti-asthma treatment or functional food. The multi-target mechanism involving oxidative stress, inflammation, and gut flora highlights AS's comprehensive therapeutic potential for asthma management.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Maternal Hyperglycemia on Fetal Pancreatic Islet Development. 母体高血糖对胎儿胰岛发育的影响。

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060728

Carina Pereira Dias, Michel Raony Teixeira Paiva de Moraes, Fernanda Angela Correia Barrence, Camila Stephanie Balbino da Silva, Basilio Smuckzec, Fernanda Ortis, Telma Maria Tenório Zorn

{"title":"Effect of Maternal Hyperglycemia on Fetal Pancreatic Islet Development.","authors":"Carina Pereira Dias, Michel Raony Teixeira Paiva de Moraes, Fernanda Angela Correia Barrence, Camila Stephanie Balbino da Silva, Basilio Smuckzec, Fernanda Ortis, Telma Maria Tenório Zorn","doi":"10.3390/biology14060728","DOIUrl":"10.3390/biology14060728","url":null,"abstract":"Hyperglycemia during fetal development disturbs extracellular matrix (ECM) synthesis and deposition patterns, which disrupts organogenesis and adult organ function. Although the ECM cooperates in pancreas development, little is known about the effects of hyperglycemia on the pancreatic ECM during development. This study investigates the effect of severe maternal hyperglycemia on ECM composition and endocrine pancreas development in E19.0 mouse fetuses. Deposition patterns of pan-laminin, laminin (alpha 1 and gamma 1 chains) and integrin alpha 3 were evaluated by immunostaining. The proliferative index of islet cells and alpha and beta cell distribution were evaluated by PCNA, glucagon and insulin immunostaining, respectively. Pdx1 and Pax4 expressions were analyzed by RT-qPCR. While for pan-laminin and laminin (alpha1 and gamma1 chains) deposition was weaker in the endocrine pancreas of hyperglycemic mothers' fetuses, integrin alpha 3 deposition in the basement membrane was increased. The proliferative index of endocrine cells was lower in the hyperglycemic group, while the beta-cell area was increased. In addition, there was a tendency towards lower Pdx1 and increased Pdx4 expression. These data suggest that maternal hyperglycemia alters fetal endocrine pancreas morphogenesis by modifying peri-islet basement membrane molecule patterns, promoting a decrease in endocrine cell proliferation associated with changes in the expression of important growth factors for the beta cells differentiated and the proliferative state.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Bacterial Microbiome of High-Moisture Plant-Based Meat Substituted Soybean Flour with Mung Bean Protein and Duckweed Powder. 用绿豆蛋白和浮萍粉替代高水分植物性肉豆粉的细菌微生物群研究

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060735

Jutamat Klinsoda, Theera Thurakit, Kullanart Tongkhao, Khemmapas Treesuwan, Kanokwan Yodin, Hataichanok Kantrong

{"title":"Exploring the Bacterial Microbiome of High-Moisture Plant-Based Meat Substituted Soybean Flour with Mung Bean Protein and Duckweed Powder.","authors":"Jutamat Klinsoda, Theera Thurakit, Kullanart Tongkhao, Khemmapas Treesuwan, Kanokwan Yodin, Hataichanok Kantrong","doi":"10.3390/biology14060735","DOIUrl":"10.3390/biology14060735","url":null,"abstract":"(1) Background: This study aimed to investigate the bacterial microbiomes in the ingredients and final PBM products during a storage period of 28 days at 2-4 °C for food safety and quality. (2) Methods: DNA from raw ingredients (i.e., defatted soy flour, potato starch, wheat gluten, mung bean protein, and duckweed) and three PBM formulations were extracted and sequenced using 16S rRNA gene sequencing. (3) Results: Alpha diversity (Simpson and Shannon) was high in the raw ingredients (p ≤ 0.05). Beta diversity showed dissimilarities between the samples. Firmicutes and Proteobacteria were the core microflora in these ingredients. The heat-stable microbes in PBM (e.g., Nostocaceae in SF and Cyanobacteriale in MB and DW) survived after extrusion. After the ingredients were stored at room temperature, the bacterial communities shifted, with Paucibacter being the majority population in raw ingredients and PBM in the 2nd batch. The predictions of Potential_Pathogens related to the abundance of Aeromonadaceae and Enterobacteriaceae need to be monitored during storage. (4) Conclusions: Our results showed that the bacterial community in PBM containing 30% MB and 3% DW did not drastically change during 28 days of storage at cold temperatures. Uncovering bacterial microbiomes in the ingredients should be emphasized for quality and safety, as ingredients influence the microbiome in the final products.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Bioactive Characterization of Berberis kaschgarica Rupr-Derived Exosome-Like Nanovesicles: Exploring Therapeutic Potential in Atherosclerosis Pathogenesis. 小檗rupr衍生的外泌体样纳米囊泡的分离和生物活性表征：探索动脉粥样硬化发病机制的治疗潜力。

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060726

Dilihuma Dilimulati, Nuerbiye Nueraihemaiti, Alhar Baishan, Sendaer Hailati, Alifeiye Aikebaier, Yipaerguli Paerhati, Wenting Zhou

{"title":"Isolation and Bioactive Characterization of Berberis kaschgarica Rupr-Derived Exosome-Like Nanovesicles: Exploring Therapeutic Potential in Atherosclerosis Pathogenesis.","authors":"Dilihuma Dilimulati, Nuerbiye Nueraihemaiti, Alhar Baishan, Sendaer Hailati, Alifeiye Aikebaier, Yipaerguli Paerhati, Wenting Zhou","doi":"10.3390/biology14060726","DOIUrl":"10.3390/biology14060726","url":null,"abstract":"Berberis kaschgarica Rupr.-derived exosome-like nanovesicles (BELNs), a type of plant-derived extracellular vesicle, consist of proteins, lipids, and nucleic acids. In this research, we employed differential centrifugation and ultracentrifugation techniques to isolate and purify BELNs. Subsequently, we conducted a comprehensive multi-omics analysis to systematically determine their physicochemical properties. Experiments were conducted in vitro with Human Umbilical Vein Endothelial Cells (HUVECs) to verify the therapeutic impact of BELNSs on atherosclerosis. The isolated BELNs exhibited a distinctive teacup-shaped exosome morphology. The extraction yield was approximately 2.1 × 1013 particles per milliliter, and the average particle size was measured to be 179.1 nm. These nanovesicles were lipid-rich. The protein content predominantly comprised cytoplasmic proteins. In-depth analysis revealed the presence of five highly conserved plant microRNAs: miR166, miR156, miR399, miR171, and miR395. These miRNAs are involved in regulating plant growth and responses to both biotic and abiotic stresses. Functional assays demonstrated that Berberis kaschgarica Rupr.-derived exosome-like nanovesicles substantially decreased the lipid deposition in HUVECs that was triggered by Palmitic Acid (PA). This research establishes the inaugural utilization of multi-omics platforms to systematically elucidate the bioactivity profile of BELNs from Berberis kaschgarica Rupr., thereby laying the groundwork for advancing its therapeutic potential.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profiling Genetic Variation: Divergence Patterns and Population Structure of Thailand's Endangered Celastrus paniculatus Willd. 遗传变异谱分析：泰国濒危野生斑芹的分化模式和种群结构。

IF 3.6 3区生物学

Biology-Basel Pub Date : 2025-06-19 DOI: 10.3390/biology14060725

Kornchanok Kaenkham, Warayutt Pilap, Weerachai Saijuntha, Sudarat Thanonkeo

{"title":"Profiling Genetic Variation: Divergence Patterns and Population Structure of Thailand's Endangered Celastrus paniculatus Willd.","authors":"Kornchanok Kaenkham, Warayutt Pilap, Weerachai Saijuntha, Sudarat Thanonkeo","doi":"10.3390/biology14060725","DOIUrl":"10.3390/biology14060725","url":null,"abstract":"This study examined genetic diversity in the endangered medicinal plant Celastrus paniculatus using 62 individual samples from seven natural populations in northern and northeastern Thailand to inform conservation strategies. The analysis of the nuclear internal transcribed spacer (ITS) and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) markers revealed 17 haplotypes (CpI1-CpI17) across these populations, with 15 being population-specific. The genetic diversity varied significantly among populations: CMI showed the highest diversity (Hd = 0.944 ± 0.070), while LEI and LPN displayed complete homogeneity. The haplotype network identified a central shared haplotype (CpI4), suggesting a common ancestry, with the PLK population showing a distinct genetic divergence through unique haplotypes separated by multiple mutation steps. Genetic distance calculations revealed close relationships between LEI and NPM populations (distance = 0.0004), with greater differentiation between PLK and other populations (distances > 0.005). Phylogenetic analyses confirmed the species integrity while highlighting population clusters, especially PLK in ITS analyses and LPN in rbcL analyses. This genetic structure information provides a foundation for targeted conservation planning. Results suggest that conservation efforts should prioritize both genetically diverse populations (like CMI and MKM) and genetically distinct ones (like PLK) to preserve the maximum evolutionary potential. This study delivers crucial molecular data for developing evidence-based conservation strategies to protect this valuable medicinal species from further decline.","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0