Biology-Basel最新文献

{"title":"Isolation, Identification, and Characterization of Novel Environmental Bacteria with Polyurethane-Degrading Activity.","authors":"Marta Muñoz-Martí, Virtudes Navarro Bañón, Mª Carmen García-Poyo, Carlos Castaño Forte, Josefina Garrido, Jose María Orts, Andrea Huguet, Jorge García-Hernández, María Ángeles Castillo","doi":"10.3390/biology14091307","DOIUrl":"10.3390/biology14091307","url":null,"abstract":"<p><p>Polyurethane (PU) is a widely used plastic material whose persistence in the environment entails a serious ecological challenge. This study aimed to isolate and characterize environmental bacteria capable of degrading PU, using Impranil DLN as a model substrate, and to investigate their enzymatic mechanisms and phylogenetic relationships. A total of 31 bacterial isolates were obtained from four waste accumulation sites and taxonomically identified across 12 different genera using MALDI-TOF MS and 16S rRNA gene sequencing. This analysis revealed genera not previously reported as PU biodegraders, including <i>Priesta</i>, <i>Dermacoccus</i>, <i>Gordonia</i>, <i>Micrococcus</i>, <i>Pseudarthrobacter</i>, and <i>Agromyces</i>. The <i>Bacillus cereus</i> clade was the most prevalent group, followed by the <i>Priestia megaterium</i> clade and <i>Achromobacter</i> sp. Biodegradation assays revealed high variability among strains, with the most efficient degrading over 90% of Impranil DLN. Protease activity was the most frequently detected enzymatic function, followed by urease and esterase activities. However, no clear correlation was observed between enzymatic profiles and degradation efficiency. Selected strains were tested on polyether PU foam, revealing biodegradative activity, an uncommon observation for bacteria on such recalcitrant material. These findings contribute to our understanding of bacterial diversity and enzymatic mechanisms involved in PU biodegradation, as well as their potential applications in plastic waste bioremediation.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects of Stem Cells from Human Exfoliated Deciduous Teeth and Their Conditioned Medium in Cisplatin-Induced Acute Kidney Injury: An In Vivo Study.","authors":"Ardeshir Talebi, Sahar Talebi, Sara Nikpoor, Nosrat Nourbakhsh, Hossein Talebi, Sareh Soroushzadeh, Miguel Gómez-Polo, Seyed Ali Mosaddad","doi":"10.3390/biology14091305","DOIUrl":"10.3390/biology14091305","url":null,"abstract":"<p><strong>Background: </strong>Acute kidney injury (AKI) is a severe condition with high morbidity and mortality. Mesenchymal stem cells (MSCs) and their conditioned medium (MSCs-CM), containing extracellular vesicles (EVs), have shown therapeutic potential. This study evaluates the effects of stem cells from human exfoliated deciduous teeth (SHED) and their conditioned medium (SHED-CM) on cisplatin-induced AKI in an in vivo model.</p><p><strong>Methods: </strong>Male Wistar rats (n = 21) were randomly divided into three groups: a control group (cisplatin-induced AKI without intervention), a SHED group (receiving intraperitoneal SHED injections), and a SHED-CM group (receiving intraperitoneal SHED-CM injections). Kidney weight, urine parameters, histopathology, and injury scores were assessed.</p><p><strong>Results: </strong>SHED-CM treatment significantly improved renal function, with reduced kidney injury scores (1.42 ± 0.18 vs. 2.57 ± 0.23 in the control, <i>p</i> < 0.05). Kidney weight was lower in the SHED-CM group compared to the control (1.12 ± 0.05 g vs. 1.36 ± 0.07 g, <i>p</i> < 0.05). SHED-CM also significantly increased urine volume (2.41 ± 0.19 mL vs. 1.23 ± 0.14 mL in the control, <i>p</i> < 0.01) and reduced urinary protein concentration (27.3 ± 2.1 mg/dL vs. 48.5 ± 3.4 mg/dL in the control, <i>p</i> < 0.05). Histopathological analysis revealed reduced tubular necrosis and inflammation, with SHED-CM showing superior improvement over SHED.</p><p><strong>Conclusions: </strong>SHED-CM demonstrated superior therapeutic efficacy over SHED injection in mitigating cisplatin-induced AKI, likely due to extracellular vesicle-mediated paracrine effects. These findings support SHED-CM as a promising, non-invasive alternative for MSC-EV-based AKI therapy.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematical Modeling of Population Dynamics of Pollinators: A Survey.","authors":"Fernando Huancas, Anibal Coronel, Esperanza Lozada, Jorge Torres","doi":"10.3390/biology14091308","DOIUrl":"10.3390/biology14091308","url":null,"abstract":"<p><p>In this paper, we develop a systematic review of the existing literature on the mathematical modeling of several aspects of pollinators. We selected the MathSciNet and Wos databases and performed a search for the words \"pollinator\" and \"mathematical model\". This search yielded a total of 236 records. After a detailed screening process, we retained 107 publications deemed most relevant to the topic of mathematical modeling in pollinator systems. We conducted a bibliometric analysis and categorized the studies based on the mathematical approaches used as the central tool in the mathematical modeling and analysis. The mathematical theories used to obtain the mathematical models were ordinary differential equations, partial differential equations, graph theory, difference equations, delay differential equations, stochastic equations, numerical methods, and other types of theories, like fractional order differential equations. Meanwhile, the topics were positive bounded solutions, equilibrium and stability analysis, bifurcation analysis, optimal control, and numerical analysis. We summarized the research findings and identified some challenges that could inform the direction of future research, highlighting areas that will aid in the development of future research.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiation-Induced EMT of Adipose-Derived Stem Cells in 3D Organotypic Culture via Notch Signaling Pathway.","authors":"Seon Jeong Choi, Meesun Kim, Kyung Tae Chung, Tae Gen Son","doi":"10.3390/biology14091306","DOIUrl":"10.3390/biology14091306","url":null,"abstract":"<p><p>In our previous study, adipose-derived stem cells (ASCs) cultured in a three-dimensional (3D) organotypic system exhibited mesenchymal-to-epithelial transition (MET) features, including cobblestone morphology and increased expression of E-cadherin and CK18. In this study, we investigated whether ionizing radiation could reverse this phenotype via epithelial-mesenchymal transition (EMT) and examined the involvement of Notch signaling. Mouse ASCs were cultured in Matrigel-based 3D organotypic conditions and exposed to 8 Gy of γ-radiation, and EMT- and Notch-related gene and protein expression were assessed 96 h post-irradiation using ATP viability assays, RT-qPCR, and Western blotting. Exposure to 8 Gy significantly reduced cell viability in 2D ASCs to 49.50 ± 6.50% compared with 61.02 ± 5.77% in 3D organoids (<i>p</i> < 0.0001). Irradiated 3D organoids showed EMT-like changes, including an increase of ~2.5-fold in fibronectin and an increase of ~2.0-fold in Twist1 expression, while epithelial CK18 was modestly elevated. Notch signaling was concurrently activated, with Notch1 and Jagged1 increasing by more than twofold and Fra-1 being significantly upregulated. Pretreatment with 20 μM of the γ-secretase inhibitor (GSI) kept cell viability above 90% and suppressed radiation-induced fibronectin, Twist1, Notch1, and Jagged1 expression. These findings indicate that ionizing radiation promotes EMT in 3D-cultured ASCs and reverses prior epithelialization, with Notch signaling playing a key regulatory role. The 3D ASC organoid model may thus provide a physiologically relevant platform for investigating radiation-induced plasticity and potential antifibrotic interventions.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bridging Neurobiology and Artificial Intelligence: A Narrative Review of Reviews on Advances in Cochlear and Auditory Neuroprostheses for Hearing Restoration.","authors":"Daniele Giansanti","doi":"10.3390/biology14091309","DOIUrl":"10.3390/biology14091309","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Hearing loss results from diverse biological insults along the auditory pathway, including sensory hair cell death, neural degeneration, and central auditory processing deficits. Implantable auditory neuroprostheses, such as cochlear and brainstem implants, aim to restore hearing by directly stimulating neural structures. Advances in neurobiology and device technology underpin the development of more sophisticated implants tailored to the biological complexity of auditory dysfunction.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;This narrative review of reviews aims to map the integration of artificial intelligence (AI) in auditory neuroprosthetics, analyzing recent research trends, key thematic areas, and the opportunities and challenges of AI-enhanced devices. By synthesizing biological and computational perspectives, it seeks to guide future interdisciplinary efforts toward more adaptive and biologically informed hearing restoration solutions.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This narrative review analyzed recent literature reviews from PubMed and Scopus (last 5 years), focusing on AI integration with auditory neuroprosthetics and related biological processes. Emphasis was placed on studies linking AI innovations to neural plasticity and device-nerve interactions, excluding purely computational works. The ANDJ (a standard narrative review checklist) checklist guided a transparent, rigorous narrative approach suited to this interdisciplinary, rapidly evolving field.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results and discussion: &lt;/strong&gt;Eighteen recent review articles were analyzed, highlighting significant advancements in the integration of artificial intelligence with auditory neuroprosthetics, particularly cochlear implants. Established areas include predictive modeling, biologically inspired signal processing, and AI-assisted surgical planning, while emerging fields such as multisensory augmentation and remote care remain underexplored. Key limitations involve fragmented biological datasets, lack of standardized biomarkers, and regulatory challenges related to algorithm transparency and clinical application. This review emphasizes the urgent need for AI frameworks that deeply integrate biological and clinical insights, expanding focus beyond cochlear implants to other neuroprosthetic devices. To complement this overview, a targeted analysis of recent cutting-edge studies was also conducted, starting from the emerging gaps to capture the latest technological and biological innovations shaping the field. These findings guide future research toward more biologically meaningful, ethical, and clinically impactful solutions.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;This narrative review highlights progress in integrating AI with auditory neuroprosthetics, emphasizing the importance of biological foundations and interdisciplinary approaches. It also recognizes ongoing challenges such as data limitations and the need for clear ethical frameworks. Collaboration acr","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Bacterial Community Characteristics of <i>Hippophae rhamnoides</i> Subsp. <i>sinensis</i> Rousi in Different Distribution Areas of the Qinghai-Tibet Plateau and Their Responses to Habitat Factors.","authors":"Pei Gao, Guisheng Ye, Yuhua Ma","doi":"10.3390/biology14091304","DOIUrl":"10.3390/biology14091304","url":null,"abstract":"<p><p>Investigating the compositional characteristics of rhizosphere soil bacterial communities of Chinese seabuckthorn across different distribution areas and their relationship with habitat factors holds significant value for the development and utilization of characteristic medicinal plant resources in the Qinghai-Tibet Plateau. This study targeted rhizosphere soil from 12 distribution areas of Chinese seabuckthorn on the Qinghai-Tibet Plateau. By integrating measurements of soil and climatic parameters with high-throughput sequencing and redundancy analysis (RDA), the research systematically elucidated the characteristics of rhizosphere bacterial communities and their environmental driving mechanisms. The results revealed that rhizosphere bacterial communities of Chinese seabuckthorn across all 12 distribution areas were dominated by Proteobacteria, Acidobacteriota, and Actinobacteriota. Among them, the R2 habitat exhibited the highest amplicon sequence variant counts (3496), while R1, R8, R9, and R12 habitats showed significantly lower counts. α/β diversity analysis revealed that Shannon, Ace, and Chao1 indices in R2 and R7 habitats were significantly higher than those in R1 and R8. Regarding community aggregation patterns, soil bacterial communities in R1, R9, and R12 habitats exhibited the highest aggregation, while those in R2 and R11 habitats showed relatively lower aggregation. Functional prediction demonstrated that Metabolism dominated across all distribution areas (50.40-52.02%), with the R11 habitat exhibiting exceptionally high metabolic function abundance (>9300). Clustering analysis partitioned the 12 habitats into two distinct groups: one comprising R2, R6, R7, R9, R10, and R11, and the other containing the remaining six habitats. Redundancy analysis (RDA) further clarified that habitat factors, including altitude, soil water content (SWC), east longitude (EAST), and pH, were key drivers shaping bacterial community structure. This study underscores the pivotal regulatory role of environmental factors in shaping rhizosphere microbial diversity, community structure, and functional profiles of Chinese seabuckthorn, thereby providing a valuable scientific foundation for the sustainable development of characteristic medicinal plant resources on the Qinghai-Tibet Plateau.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Provenance-Specific Height-Diameter Modeling for Chinese Fir: A Clustered Mixed-Effects Approach.","authors":"Xiangrong Wu, Yuhan Wang, Yanjuan Lyu, Wanrong Chen, Ming Li, Shuaichao Sun","doi":"10.3390/biology14091301","DOIUrl":"10.3390/biology14091301","url":null,"abstract":"<p><p>Chinese fir is the predominant afforestation species in southern China, exhibiting distinct provenances due to long-term climatic adaptation. This study utilized data from four surveys conducted at different ages in a provenance trial forest at Zhangping Wuyi Forest Farm, Fujian Province, to classify Chinese fir provenances using cluster analysis based on growth metrics. The resulting clusters were integrated as random effects into height-diameter models. Model performance was enhanced by incorporating age parameters and validated through five-fold cross-validation. The findings reveal that: (1) the Logistic model best captured the fundamental height-diameter relationship of Chinese fir; (2) the inclusion of provenance-clustering random effects improved model fit and predictive accuracy, with height-based clustering outperforming other methods; (3) the addition of age parameters further refined the base models beyond the clustering effects, and the combination of both approaches achieved the highest precision. Among clustering techniques, height-based clustering surpassed combined height-diameter at breast height (DBH) clustering, while DBH-based clustering was the least effective. The developed models facilitate precise growth predictions for multi-provenance Chinese fir across extensive geographic ranges, offering a theoretical basis for provenance-specific management.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosensor-Based Comparison of Stress Responses in Qingtian Paddy Field Carp (<i>Cyprinus carpio</i> var. <i>qingtianensis</i>) and Xingguo Red Carp (<i>Cyprinus carpio</i> var. <i>singuonensis</i>) Under Acute Shallow Water Conditions.","authors":"Tengyu Liu, Rui Han, Yuhan Jiang, Jiamin Sun, Haiyun Wu, Qigen Liu","doi":"10.3390/biology14091303","DOIUrl":"10.3390/biology14091303","url":null,"abstract":"<p><p>The domestication of common carp in rice paddies (5-20 cm depth) is challenging, as the fish must withstand drastic fluctuations in temperature and dissolved oxygen, restricted movement, and bird predation without the option of diving. The effects of stress responses in different species of carp in shallow-water environments remain poorly understood, particularly with fluctuating water levels where real-time monitoring is challenging. This study employed a glucose biosensor system enabling real-time monitoring, together with biochemical analysis techniques capable of evaluating multiple physiological indicators, to investigate shallow-water adaptation in Qingtian paddy field carp and Xingguo red carp. Our results quantitatively reveal, for the first time, the differing physiological stress thresholds of the two carp strains under shallow water. The Qingtian paddy field carp exhibited a higher tolerance to shallow water and showed faster recovery from prolonged stress. Furthermore, the total cholesterol and triglyceride contents of Qingtian paddy field carp gradually increased with prolonged shallow-water stress, reflecting the activation of lipid metabolic pathways. These findings highlight the advantages of biosensor technology in aquatic stress research and a strong support of the core element of paddy domesticated carp in the Globally Important Agricultural Heritage Systems.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the Complete Mitogenome of <i>Polypedates braueri</i> (Anura, Rhacophoridae, <i>Polypedates</i>) and Insights into the Phylogenetic Relationships of Rhacophoridae.","authors":"Simin Chen, Huiling Huang, Siqi Shan, Chengmin Li, Kaiyuan Huang, Xinyi Xu, Lichun Jiang","doi":"10.3390/biology14091299","DOIUrl":"10.3390/biology14091299","url":null,"abstract":"<p><p>White-lipped tree frogs, <i>Polypedates braueri</i>, are currently included in the list of terrestrial wildlife with important ecological, scientific, and social value in China. Understanding the structure and characteristics of the mitochondrial genome provides essential information for resource conservation and phylogenetic analyses of <i>P. braueri</i>. While the complete mitochondrial genomes serve as important molecular markers for phylogenetic and genetic studies, the mitochondrial genome of <i>P. braueri</i> has received little attention. In this paper, we analyzed the characterization of the mitochondrial genome of <i>P. braueri</i> and investigated the phylogenetic relationships of Rhacophoridae. The complete mitochondrial genome of <i>P. braueri</i> was 20,254 bp in length, containing thirty-six genes (twelve protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), twenty-two transfer RNA genes (tRNAs)), three non-coding regions for the origin of light strand (<i>O_L</i>), and two control regions (<i>CR</i>). There were six overlapping regions and seventeen intergenic spacer regions in the mitogenome. The mitogenome also showed a bias towards A + T content (61.87%) and had negative AT-skew (-0.039) and GC-skew (-0.209). All the PCGs employed the ATG, ATA, or ATT as the start codon and TAA, TAG, AGG, or single T as the stop codon. Additionally, all of the tRNAs displayed a typical cloverleaf secondary structure, except trnS1, which lacked the D arm. The phylogenetic analysis, based on the maximum likelihood (ML) and Bayesian inference (BI) methods, revealed that Rhacophoridae could be classified into four monophyletic genera. The phylogenetic status of <i>P. braueri</i> was closely related to that of <i>Polypedates megacephalus</i> and <i>Polypedates leucomystax</i>. Additionally, selective pressure analysis suggested that <i>COX1</i> and <i>ND1</i> were highly efficient for discriminating closely related species in the genus <i>Polypedates</i>, while <i>ND4L</i> was the most appropriate marker for population-level genetic analyses. The diversification of the <i>Polypedates</i> commenced during the Late Oligocene and extended into the Miocene. The present study provides valuable genomic information on <i>P. braueri</i> and new insights into the phylogenetic relationships of Rhacophoridae.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Physiological and Antioxidant Properties of the Medicinal and Endemic <i>Hypericum bilgehan-bilgilii</i> Species Under Different Cultivation Methods.","authors":"Huseyin Turker","doi":"10.3390/biology14091302","DOIUrl":"10.3390/biology14091302","url":null,"abstract":"<p><p><i>Hypericum bilgehan bilgilii</i> is a locally endemic species belonging to the genus <i>Hypericum</i>. Considering that the related plant has pharmacological and medicinal potential like other <i>Hypericum</i> species, this study has been conducted to determine its physiological and antioxidant activities. Plant extracts obtained through three different cultivation methods (wild, cultivated in a laboratory environment in peat, and cultivated using tissue culture methods) were used to determine physiological, biochemical, and antioxidant activity analyses. (The abbreviations for the cultivation methods are indicated as, respectively, W, in vivo in lab, in vitro in lab). In this study, the highest photosynthetic pigment content, total protein content, proline content, malondialdehyde (MDA) content, phenylalanine ammonium lyase (PAL) activity, antioxidant activity (2,2-Diphenyl-1-picryl hydrazyl radical scavenging activity, total phenolic content, carotenoid) values were determined for the first time in the in vitro in lab group in <i>H. bilgehan bilgilii</i> plants grown under three different cultivation methods. Superoxide dismutase (SOD) and catalase (CAT) enzyme activities were observed at the highest levels in the wild group. Based on these parameters, the physiological, biochemical, and antioxidant activities of the <i>H. bilgehan bilgilii</i> plant were studied for the first time under different cultivation conditions, producing new results. According to these results, it was determined that in vitro cultivated plants have higher physiological parameters and antioxidant content. This study has also demonstrated the significance of tissue culture, a biotechnological method, in enhancing bioactive compounds, and that in vitro cultures could be used as an alternative to traditional methods for producing larger quantities of bioactive compounds. In addition, establishing in vitro cultures for endemic, endangered, and medically significant species will enable the sustainable use of the local endemic species <i>H. bilgehan-bilgilii</i>, facilitate large-scale production of phytochemicals, and provide a valuable plant resource and information for future comprehensive phytochemical studies.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}