Biology-Basel最新文献

{"title":"Induction of Sustained Immunity Following Vaccination with Live Attenuated <i>Trypanosoma cruzi</i> Parasites Combined with Saponin-Based Adjuvants.","authors":"Brenda A Zabala, María Elisa Vázquez, Daniela E Barraza, Andrea C Mesías, Federico Ramos, Alejandro Uncos, Iván S Marcipar, Leonardo Acuña, Cecilia Pérez Brandán","doi":"10.3390/biology14091298","DOIUrl":"10.3390/biology14091298","url":null,"abstract":"<p><p>Chagas disease, caused by <i>Trypanosoma cruzi</i>, remains a major health concern in Latin America, particularly affecting low-income and rural communities. Among the many vaccine strategies explored, live attenuated parasites have shown the strongest ability to trigger protective immune responses. In this study, we investigated whether adding saponin-based adjuvants-Immunostimulant Particle Adjuvant (ISPA) and Quil-A-could improve the effectiveness and safety of a live parasite attenuated <i>T. cruzi</i> vaccine. Mice were vaccinated with a <i>T. cruzi</i> attenuated strain (TCC) alone or in combination with each adjuvant, and immunoglobulin G (IgG) subtypes in the serum of vaccinated mice, and interferon gamma (IFN-γ) and interleukin-10 (IL-10) in the supernatants of stimulated cells were measured at two weeks and twelve months post-vaccination. While protection levels were similar across all groups, the adjuvants assist in modulating the immune response over time: ISPA and Quil-A initially shifted antibody production toward IgG1 but later favored a balanced T_H1/T_H2 profile. ISPA also promoted long-term regulation through increased IL-10. Both adjuvants reduced tissue inflammation and enhanced clearance of vaccine-derived parasites. These findings suggest that while adjuvants may not boost protection directly, they significantly improve vaccine safety and immune quality, reinforcing their value in developing better vaccines for Chagas disease.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of Placental Vitamin D Receptor Expression in Gestational Diabetes Is Not Directly Related to Vitamin D Concentration.","authors":"Marek Walkowiak, Małgorzata Jamka, Mateusz de Mezer, Jakub Żurawski, Paweł Gutaj, Ewa Wender-Ożegowska","doi":"10.3390/biology14091300","DOIUrl":"10.3390/biology14091300","url":null,"abstract":"<p><p>Vitamin D plays a key role in pregnancy beyond calcium-phosphate regulation, modulating immune responses and glucose metabolism via the vitamin D receptor (VDR). Placental expression may be altered in gestational diabetes mellitus (GDM). This study aimed to assess placental VDR expression in GDM and evaluate its association with maternal vitamin D levels and clinical parameters. VDR expression in the placental tissue of 53 women with GDM and 26 healthy controls was assessed semi-quantitatively by immunohistochemistry. Maternal serum 25-hydroxyvitamin D (25(OH)D) levels, body mass index, weight gain, neonatal outcomes, and other variables were evaluated. Univariate and multivariate linear regression analyses were performed. VDR expression was significantly higher in the GDM group compared to controls (<i>p</i> = 0.0297 for mean, <i>p</i> = 0.0378 for median). No significant differences were observed in serum 25(OH)D concentrations between groups. Stepwise regression revealed that diabetes was the only independent predictor of VDR expression. Within the GDM subgroup, VDR expression was not associated with any clinical parameters, including maternal vitamin D status. Upregulated placental VDR in GDM may represent an adaptive response to metabolic stress. These findings suggest complex regulation of vitamin D signalling in diabetic pregnancies, warranting further investigation.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium and Mammalian Uterine Health: A Comprehensive Review.","authors":"Ruoning Wu, Xiaohan Li, Zhaoming Li, Jilong Luo, Ziwei Zhang, Mengyao Guo","doi":"10.3390/biology14091295","DOIUrl":"10.3390/biology14091295","url":null,"abstract":"<p><p>Selenium (Se) is an essential trace element for animals and plays an important role in maintaining various physiological functions. In recent years, more and more studies have focused on the relationship between selenium and mammalian uterus health. This review comprehensively summarizes the current research progress in this field and introduces the physiological functions of selenium, including its antioxidant, anti-inflammatory, and immune regulatory functions. It also analyzed the role of selenium in uterine development, maintaining pregnancy, preventing and treating uterine diseases from multiple aspects. In addition, factors affecting animal selenium utilization and appropriate selenium supplementation strategies were also introduced. Understanding the relationship between selenium and animal uterine health is of great significance for improving animal reproductive performance, ensuring offspring health, and promoting sustainable development of the animal husbandry.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal Production and Quality in the Red Sea Urchin <i>Mesocentrotus franciscanus</i> Fed with Seaweed <i>Devaleraea mollis</i> and <i>Ulva australis</i> from a Land-Based Integrated Multi-Trophic Aquaculture (IMTA) System.","authors":"Matthew S Elliott, Yuanzi Huo, Mark Drawbridge","doi":"10.3390/biology14091294","DOIUrl":"10.3390/biology14091294","url":null,"abstract":"<p><p>Harvesting sea urchins from barrens and enhancing their gonads through aquaculture offers a promising way to convert low-value individuals into high-quality seafood. This study evaluated whether red sea urchins (<i>Mesocentrotus franciscanus</i>, RSUs) fed nutrient-enriched seaweeds produced in a land-based integrated multi-trophic aquaculture (IMTA) system could significantly improve gonad size and quality. Two seaweed species, <i>Ulva australis</i> and <i>Devaleraea mollis</i>, were grown in effluent from white seabass (<i>Atractoscion nobilis</i>) tanks and used to feed RSUs over an 8-week period. RSUs readily consumed both seaweeds, with measurable ingestion, fecal output, and absorption efficiency. We hypothesized that RSUs fed IMTA seaweed would exhibit increased gonadosomatic index and improved gonad quality. GSI significantly increased in both groups, from an initial 3.00 ± 0.50% (9.02 ± 1.80 g) to 4.64 ± 0.66% (23.04 ± 10.20 g) in the <i>U. australis</i> group and to 6.35 ± 1.30% (31.20 ± 7.20 g) in the <i>D. mollis</i> group. Gonad quality improved from \"D\" (unmarketable) to average \"B\" (high-quality) grade, based on color, firmness, and texture. These results demonstrate that RSUs collected from barrens can be enhanced into premium seafood using nutrient-enriched seaweeds. Integrating RSUs into land-based IMTA systems may increase aquaculture efficiency, reduce waste, and diversify seafood production in a sustainable and economically viable way.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly and Analysis of the Complete Mitochondrial Genome of <i>Eryngium foetidum</i> L. (Apiaceae).","authors":"Lihong Zhang, Wenhu Zhang, Yongjian Luo, Jun Liu, Qing Li, Qiongheng Liu","doi":"10.3390/biology14091296","DOIUrl":"10.3390/biology14091296","url":null,"abstract":"<p><p><i>Eryngium foetidum</i> L. belongs to the Apiaceae family and is a perennial herb. The entire plant is rich in essential oils, which have a distinctive aroma similar to cilantro. This plant exhibits significant biological activity and possesses characteristics such as disease resistance and antimicrobial properties, showing great potential in medical and food applications. Additionally, its essential oil has substantial commercial value. Mitochondria play a crucial role as organelles within plant cells; however, the mitochondrial genome of <i>E. foetidum</i> remains underexplored. To fill this research gap, we conducted sequencing and assembly of the mitochondrial genome of <i>E. foetidum</i>, aiming to uncover its genetic mechanisms and evolutionary trajectories. Our investigation reveals that the mitochondrial genome of <i>E. foetidum</i> is a circular structure, similar to that of other species, with a length of 241,660 bp and a GC content of 45.35%, which is within the range observed in other organisms. This genome encodes 59 genes, comprising 37 protein-coding sequences, 18 tRNA genes, and 4 rRNA genes. Comparative analysis highlighted 16 homologous regions between the mitochondrial and chloroplast genomes, with the longest segment spanning 992 bp. By analyzing 37 protein-coding genes (PCGs), we identified 479 potential RNA editing sites, which induce the formation of stop codons in the <i>nad3</i> and <i>atp6</i> genes, as well as start codons in the <i>ccmFC</i>, <i>atp8</i>, <i>nad4L</i>, <i>cox2</i>, <i>cox1</i>, and <i>nad7</i> genes. Meanwhile, the genome shows a preference for A/T bases and A/T-ending codons, with 32 codons having a relative synonymous codon usage (RSCU) value greater than 1. The codon usage bias is relatively weak and mainly influenced by natural selection. Most PCGs are under purifying selection (Ka/Ks < 1), while only a few genes, such as <i>rps7</i> and <i>matR,</i> may be under positive selection. Phylogenetic analysis of mitochondrial PCGs from 21 species showed <i>E. foetidum</i> at the basal node of Apiaceae, consistent with the latest APG angiosperm classification and chloroplast genome-based phylogenetic relationships. In summary, our comprehensive characterization of the <i>E. foetidum</i> mitochondrial genome not only provides novel insights into its evolutionary history and genetic regulation but also establishes a critical genomic resource for future molecular breeding efforts targeting mitochondrial-associated traits in this economically important species.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Stone Moroko <i>Pseudorasbora parva</i> Altered the Composition and Stability of Sediment Microbial Communities Within the Chinese Mitten Crab (<i>Eriocheir sinensis</i>) Polyculture Pond.","authors":"Yiran Hou, Yun Bao, Rui Jia, Linjun Zhou, Lili Song, Baojuan Yang, Bing Li, Jian Zhu","doi":"10.3390/biology14091297","DOIUrl":"10.3390/biology14091297","url":null,"abstract":"<p><p>Integrated aquaculture, centered around polyculture involving multiple species, is a typical practice for the sustainable development of the aquaculture industry, capable of enhancing resource utilization efficiency, environmental stability, and overall productivity through establishing symbiotic interactions among species. This study employed multi-amplicon high-throughput sequencing to assess the ecological impacts of two polyculture methods involving river crabs on sediment bacteria, fungi, and protists. One method involved polyculturing river crabs with mandarin fish, silver carp, and the stone moroko (SPC), and the other involved polyculturing river crabs with only mandarin fish and silver carp (SMC). The results showed that, compared to the SMC group, the SPC group remarkably increased the Chao1 index of bacterial communities in pond sediment and decreased the Pielou_J index of protists. The relative abundances of all fungal phyla and most dominant bacterial and protistan phyla (top 10 in relative abundance) in the SPC group were considerably different from those in the SMC group. In the co-occurrence networks of bacterial, fungal, and protistan communities, the numbers of edges and nodes were higher in the SPC group than in the SMC group, and the habitat niche breadth of bacterial community was also notably increased in the SPC group. The levels of total carbon (TC), total nitrogen (TN), and phosphates within pond sediment in the SPC group were obviously lower than those in the SMC group, and were significantly correlated with the microbial communities, with TC being identified as the primary contributor driving changes in the microbial communities. All the findings collectively demonstrate that the polyculture of river crabs with mandarin fish, silver carp, and the stone moroko enhances the stability of bacterial, fungal, and protistan communities in sediment and enhances resource utilization efficiency in aquaculture, thereby preventing the environmental risks associated with excessive nutrient accumulation in sediment. Polyculture systems integrating river crabs with mandarin fish, silver carp, and the stone moroko represent a sustainable aquaculture model with significant ecological benefits.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Analysis of <i>TaNCED</i> Gene Family in Wheat Vernalization Process.","authors":"Guoqing Cui, Hao Cheng","doi":"10.3390/biology14091293","DOIUrl":"10.3390/biology14091293","url":null,"abstract":"<p><p>9-<i>cis</i>-epoxycarotenoid dioxygenases (NCEDs), serving as the rate-limiting enzymes in abscisic acid (ABA) biosynthesis, play a pivotal role in regulating plant growth and development, as well as responses to abiotic stresses. Despite their agronomic importance, the molecular dialog between ABA signaling and vernalization, a cold-induced switch from vegetative to reproductive growth in wheat, remains poorly characterized, particularly regarding the <i>TaNCED</i> gene family members. Here, we systematically identified 13 <i>TaNCED</i> members in hexaploid wheat, followed by multi-omics characterization encompassing physicochemical properties, exon-intron architectures, conserved catalytic domains, protein motifs, and <i>cis</i>-acting elements. By analyzing transcriptome data from vernalization treatments, we profiled the expression patterns of <i>TaNCED</i> genes during vernalization. Notably, <i>TaNCED5-6A</i>, <i>TaNCED5-6B</i>, and <i>TaNCED5-6D</i> exhibited significant upregulation in vernalized leaves and tiller buds, while maintaining basal expression in the shoot apical meristem, the site of floral induction. This tissue-specific expression pattern implicates their specialized role in mediating vernalization responses via ABA biosynthesis. Collectively, our findings provide novel insights into the regulatory mechanisms of ABA-mediated vernalization in wheat and offer valuable targets for vernalization efficiency in cereal breeding programs.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Dual Role of <i>RASSF4</i> in Tumorigenesis: Mechanisms and Epigenetic Targeting Strategies.","authors":"Rui Tian, Yixin Wu, Wenbin Yuan, Lingli Tian, Rui Zhang, Hao Lyu, Shuai Xiao, Dong Guo, Qi Zhang, Declan William Ali, Marek Michalak, Cefan Zhou, Jingfeng Tang, Xing-Zhen Chen","doi":"10.3390/biology14091289","DOIUrl":"10.3390/biology14091289","url":null,"abstract":"<p><p>RASSF4 is a key member of the Ras-associated domain family (RASSF) that exhibits dual functionality in tumorigenesis, playing critical yet context-dependent roles in various malignancies. Its expression is epigenetically regulated through promoter hypermethylation, histone modifications, and microRNAs including miR-155 and miR-196a-5p, which directly target its 3' untranslated region. In most cancers, such as non-small cell lung cancer (NSCLC) and gastric adenocarcinoma (GAC), RASSF4 acts as a tumor suppressor by inhibiting the RAS/MAPK pathway while activating the Hippo signaling cascade, ultimately inducing cell cycle arrest and apoptosis. Conversely, in aRMS, RASSF4 is upregulated by the PAX3-FOXO1 fusion oncoprotein and promotes tumor growth through MST1 inhibition and subsequent YAP activation. This review systematically analyzes current evidence regarding RASSF4's complex regulatory mechanisms and clinical significance. We propose targeted therapeutic strategies including epigenetic reactivation, gene intervention, and combination therapies. Furthermore, we identify RASSF4 as a promising diagnostic biomarker and therapeutic target based on integrated mechanistic and clinical evidence. Future research should focus on elucidating context-dependent regulatory switches, developing targeted delivery systems, and validating clinical utility through prospective trials.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urban Edge Predators: Wolf Spatial and Temporal Ecology at the Wildland-Urban Interface in Mongolia.","authors":"Jeff Dolphin, Maria Vittoria Mazzamuto, Gantulga Gankhuyag, Delgerchimeg Davaasuren, Bayaraa Munkhtsog, Ulam-Urnukh Bayanmunkh, Gansukh Sukhchuluun, John L Koprowski","doi":"10.3390/biology14091292","DOIUrl":"10.3390/biology14091292","url":null,"abstract":"<p><p>Mongolia's rapidly expanding capital is encroaching on Bogd Khan Mountain, a UNESCO Biosphere Reserve and the oldest protected area in Eurasia. Gray wolves (<i>Canis lupus</i>) in this wildland-urban interface are locally near-threatened due to hunting, local beliefs, and human-wildlife conflict. In 2022 and 2023, we deployed 72 camera traps (11,539 trap nights) to investigate how wolves respond to overlapping pressures from free-ranging dogs, livestock, and human activity. Using a random habitat-stratified camera design and abundance modeling, we assessed diel activity and spatial co-occurrence. Wolves exhibited nocturnal and crepuscular activity, with the greatest temporal overlap with wild prey (wapiti: ∆⁴ = 0.73; Siberian roe deer: ∆⁴ = 0.79), moderate overlap with dogs (∆⁴ = 0.60) and horses (∆⁴ = 0.68), and minimal overlap with cattle (∆⁴ = 0.40) and people (∆⁴ = 0.43). Mean wolf abundance estimates ranged from λ = 0.91 (CI 95%, 0.05-1.77) in 2022 to λ = 1.52 (CI 95%, 0.44-3.53) in 2023. Wolves were more abundant at higher relative abundance of wild ungulates and in areas with more people. Wolves co-occurred with dogs at 11 sites and were more abundant in areas with a higher number of dogs. Our findings highlight the complex dynamics between wildlife, livestock, and human-associated disturbances at the wildland-urban interface, underscoring the need for integrated management strategies that address both ecological and human dimensions of conservation.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Welander Distal Myopathy-Associated TIA1 E384K Mutation Disrupts Stress Granule Dynamics Under Distinct Stress Conditions.","authors":"Beatriz Ramos-Velasco, José Alcalde, José M Izquierdo","doi":"10.3390/biology14091288","DOIUrl":"10.3390/biology14091288","url":null,"abstract":"<p><p>Cellular stress triggers the formation of diverse RNA-protein aggregates, which can be associated with physiological responses, pathological conditions, or even detrimental outcomes. Under stress-induced proteostasis disruption, these RNA-protein assemblies are known as stress granules (SGs). Targeting such condensates-while sparing functional RNAs and proteins-remains a major therapeutic challenge in protein aggregation disorders such as myopathies and neuropathies. In this study, we investigated the cellular response to various stress conditions in the context of the TIA1 E384K mutation, a founder variant implicated in both Welander distal myopathy (WDM) and amyotrophic lateral sclerosis (ALS). Cells were exposed to different stressors, including proteotoxic, proteostatic, chemotoxic, and osmotic insults, and the behavior of TIA1-related SGs was analyzed. Our findings reveal a distinct yet conserved pattern in the dynamics of TIA1-dependent SG formation and clearance, influenced by the specific type of stressor and modulated by eIF2α Ser35 phosphorylation. These results indicate that the WDM-associated TIA1 mutation leads to aberrant SG dynamics across different stress conditions. Collectively, these observations support the idea that TIA1 E384K-associated SG dysregulation plays a role in WDM and ALS pathogenesis and underscores the importance of multiple stress contexts in disease progression.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 9","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12467723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145179603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}