Biology-Basel最新文献

{"title":"Gait Asymmetry and Post-Traumatic Osteoarthritis Following Anterior Cruciate Ligament Rupture: A Preliminary Study.","authors":"Samuel Pringle, Kristiaan D'Août","doi":"10.3390/biology14020208","DOIUrl":"10.3390/biology14020208","url":null,"abstract":"<p><p>Knee post-traumatic osteoarthritis (PTOA) often develops in younger populations following anterior cruciate ligament (ACL) rupture, accounting for 12% of all symptomatic osteoarthritis (OA). The current literature implicates gait asymmetry in late-stage knee OA progression; however, early-knee PTOA development involvement is ill defined. This study explored gait asymmetry involvement in early-stage knee PTOA following ACL ruptures. Gait asymmetry, measured as asymmetry in duty factor (relative contact time), and joint loading data were collected, using infrared-camera motion capture and Kistler force plates for participants exhibiting either historical ACL ruptures (ACL+; <i>n</i> = 4) or no previous joint trauma (ACL-; <i>n</i> = 11). Joint loading measures included external knee adduction moment (EKAM) and external knee flexion moment (KFM), early (peak 1; EKAMp1 and KFMp1) and late (peak 2; EKAMp2 and KFMp2), stance peaks (Nm/kg), and respective time integrals (Nm·ms/kg; iEKAMp1, iEKAMp2, iKFMp1, and iKFMp2). ACL+ exhibited greater asymmetrical duty factor (78% difference) and greater joint load differences: EKAMp1 (26%), EKAMp2 (49%), KFMp1 (37%), iKFMp1 (44%), and iKFMp2 (60%). Significant relationships were found between duty factor asymmetry and both KFMp2 (R² = 0.665) and iKFMp2 (R² = 0.504). These preliminary data suggest gait asymmetry-induced joint loading may contribute to knee PTOA progression, but further research with increased sample sizes and the quantitative assessment of cartilage status is required.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Clinopathological and Prognostic Significance of <i>SPOCK1</i> in Gynecological Cancers: A Bioinformatics Based Analysis.","authors":"Enes Karaman, Fatih Yay, Durmus Ayan, Ergul Bayram, Sefa Erturk","doi":"10.3390/biology14020209","DOIUrl":"10.3390/biology14020209","url":null,"abstract":"<p><p><b>Background:</b> Sparc/osteonectin, cwcv, and kazal-like domains proteoglycan 1 (<i>SPOCK1</i>) is an oncogene that promotes tumor formation and progression in certain types of cancer and is associated with poor survival rates. However, there is limited information on the importance of <i>SPOCK1</i> in gynecological cancers in the literature. The aim of this study was to explore the role of <i>SPOCK1</i> in ovarian serous cystadenocarcinoma (OV), cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), and uterine corpus endometrial carcinomas (UCEC). <b>Methods</b>: The data used in this study were obtained from the GEPIA2, TCGA, Kaplan-Meier Plotter, GeneMANIA, UALCAN, cBioPortal, and TIMER databases. Overall survival (OS) and relapse-free survival (RFS) rates were evaluated by Kaplan-Meier survival analysis. Spearman's rho and statistical significance values were obtained for the correlation between <i>SPOCK1</i> expression and tumor infiltration by different immune cells. <b>Results</b>: Lower <i>SPOCK1</i> gene expression was observed in CESC and UCEC compared to normal tissue (<i>p</i> < 0.05), but the OV did not differ significantly (<i>p</i> > 0.05). In OV, <i>SPOCK1</i> gene expression was solely linked to age; in CESC, it was linked to age, stage, weight, and histology; and in UCEC, it was linked to age, stage, weight, and menopausal status. <b>Conclusions:</b><i>SPOCK1</i> gene expression in UCEC showed weak positive correlations with CD8+ T cells and weak negative correlations with CD4+ T cells. <i>SPOCK1</i> may be a potential prognostic and therapeutic target for gynecological cancers.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Effects of Adverse Maternal Care on Hypothalamic-Pituitary-Adrenal (HPA) Axis Function of Juvenile and Adolescent Macaques.","authors":"Kai McCormack, Sara Bramlett, Elyse L Morin, Erin R Siebert, Dora Guzman, Brittany Howell, Mar M Sanchez","doi":"10.3390/biology14020204","DOIUrl":"10.3390/biology14020204","url":null,"abstract":"<p><p>Early life adversity (ELA) is a known risk factor for psychopathology, including stress-related anxiety and depressive disorders. The underlying mechanisms and developmental changes remain poorly understood. A likely underpinning is the impact of ELA on the development of stress response systems, including the hypothalamic-pituitary-adrenal (HPA) axis. Our group studied a translational ELA model of spontaneous infant maltreatment by the mother in rhesus macaques, where we used a cross-fostering design to randomly assign infant macaques to either Control or Maltreating (MALT) foster mothers at birth to examine the impact of adverse caregiving on the development of the HPA axis, while controlling for the confounding effects of heritable and prenatal factors. We previously reported higher levels of plasma and hair cortisol (CORT) across the first 6 postnatal months (equivalent to the first 2 years of life in humans) in the MALT than in the Control infants. Here, we followed the same cohort of infants longitudinally to assess the long-term developmental impact of this adverse experience on HPA axis function during the juvenile (12, 18 months) and late adolescent (~5 years) periods. For this, we collected measurements of diurnal CORT rhythm and glucocorticoid negative feedback using the dexamethasone suppression test (DST). At 12 months, we found higher diurnal CORT secretion in MALT females compared to Control females, and impaired negative feedback in response to the DST in both sexes in the MALT group. However, ELA group differences in the HPA axis function disappeared by 18 months and late adolescence, while sex differences in diurnal CORT rhythm emerged or became stronger. These results suggest that infant maltreatment causes dysregulation of the HPA axis during the first year of life, with HPA axis function normalizing later, during the pre-pubertal juvenile period and adolescence. This suggests that the impact of maltreatment on HPA axis function may be transient, at least if the adverse experience stops. Our findings are consistent with human evidence of recalibration/normalization of HPA axis function during adolescence in children that switch from adverse/deprived environments to supportive adoptive families. This research has broad implications regarding the biological processes that translate ELA to psychopathology during development and the pathways to resiliency.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Difference Analysis Among Six Kinds of Acceptor Splicing Sequences by the Dispersion Features of 6-mer Subsets in Human Genes.","authors":"Yangming Si, Hong Li, Xiaolong Li","doi":"10.3390/biology14020206","DOIUrl":"10.3390/biology14020206","url":null,"abstract":"<p><p>Identifying the sequence composition of different splicing modes is a challenge in current research. This study explored the dispersion distributions of 6-mer subsets in human acceptor splicing regions. Without differentiating acceptor splicing modes, obvious differences were observed across the upstream, core, and downstream regions of splicing sites for 16 dispersion distributions. These findings indicate that the dispersion value of each subset can effectively characterize the compositional properties of splicing sequences. When acceptor splicing sequences were classified into common, constitutive, and alternative modes, the differences in dispersion distributions for most of the XY1 6-mer subsets were significant among the three splicing modes. Furthermore, the alternative splicing mode was classified into normal, exonic, and intronic sub-modes, the differences in dispersion distributions for most of the XY1 6-mer subsets were also significant among the three splicing sub-modes. Our results indicate that dispersion values of XY1 6-mer subsets not only revealed the sequence composition patterns of acceptor splicing regions but also effectively identified the differences in base correlation among various acceptor splicing modes. Our research provides new insights into revealing and predicting different splicing modes.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Analysis of Potential Aquaculture Spaces for <i>Larimichthys crocea</i> in China's Surrounding Waters Based on Environmental Temperature Suitability.","authors":"Ling Yang, Weifeng Zhou, Xuesen Cui, Yanan Lu, Qin Liu","doi":"10.3390/biology14020205","DOIUrl":"10.3390/biology14020205","url":null,"abstract":"<p><p>This research evaluates the potential spaces of deep offshore waters for cultivating the <i>Larimichthys crocea</i>, analyzing ocean profile temperature data from 2000 to 2022 according to the species' environmental temperature suitability. There are significant seasonal variations and differences in habitat distributions of different temperature ranges in China's surrounding waters. The range of maximum living space obtained according to the tolerance temperature shows a trend of being larger in summer and smaller in winter; and the range of viable habitat space obtained based on the suitable and optimal temperature shows a trend of being smaller in summer and larger in winter. Broad areas meeting tolerance temperatures offer broad, yet impractical, site selection options. In contrast, areas with optimal temperatures are limited, which means the availability of ideal site locations is very restricted. Regions consistently within the 20-28 °C range are best for practical site selection. Year-round suitable areas are primarily found at depths of 30 to 90 m in the southern East China Sea and the South China Sea, particularly within the 40 to 50 m depth range. Water mass like the South China Sea Surface Water and the Kuroshio Surface Water consistently maintain suitable temperatures, making them ideal for aquaculture.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Causal Relationship Between Plasma Proteins and Pulmonary Fibrosis: A Systematic Analysis Based on Mendelian Randomization.","authors":"Moxuan Han, Yan Cui, Zhengyuan Fang, He Li, Yueqi Wang, Mingwei Sima, Yan Bi, Donghui Yue","doi":"10.3390/biology14020200","DOIUrl":"10.3390/biology14020200","url":null,"abstract":"<p><p>Pulmonary fibrosis (PF) is a chronic interstitial lung disease characterized by the destruction of alveolar structures, the abnormal accumulation of extracellular matrix (ECM), and ultimately respiratory failure. Although previous studies have shown that plasma proteins play an important role in the onset and progression of PF, there is currently a lack of systematic studies on causal relationships. To address the identified gap, the study employs the MR method to identify potential drug targets associated with PF. Plasma protein data (pQTL, exposure) were sourced from Ferkingstad et al. (<i>n</i> = 35,559), and PF-related summary statistics were obtained from the GWAS database (<i>n</i> = 469,126). The study integrates enrichment analysis, protein-protein interaction (PPI) networks, drug prediction, molecular docking, and single-cell sequencing to further evaluate the biological functions and pharmacological potential of the identified targets. In the MR analysis, 64 genetic loci were significantly associated with the occurrence of PF. Further reverse Mendelian analysis revealed a positive causal relationship between PF and genes such as <i>NPTX1</i>, <i>IL31</i>, and <i>CTSE</i>, suggesting that these proteins may play a promotive role in the onset and progression of pulmonary fibrosis. The PPI network analysis identified core genes such as <i>CDH1</i>, <i>CRP</i>, <i>VTN</i>, <i>COL1A1</i>, and <i>MAPK8</i>, which are involved in the key pathological processes of PF, including cell signaling, ECM remodeling, and immune responses. The drug prediction analysis identified potential drugs such as sorafenib, vitamin C, and vitamin E, and the molecular docking analysis showed good binding between the drugs and the proteins. The single-cell sequencing results showed that core genes were highly expressed in fibroblasts and alveolar type II cells, confirming their potential role in the pathogenesis of PF. This study successfully identifies 64 potential drug targets for PF, with 10 core targets considered particularly promising for clinical trials. These findings offer valuable insights into the molecular mechanisms underlying PF and open new avenues for the development of targeted therapies. This research may accelerate the development of effective PF treatments and reduce drug development costs by providing more precise and personalized approaches to managing the disease.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of <i>Centella asiatica</i> Against Senescence and Apoptosis in Epidermal Cells.","authors":"Yu Tan, Ailing Hu, Jingya Lu, Yunhai Lin, Xuejing Li, Takuji Yamaguchi, Masahiro Tabuchi, Zenji Kawakami, Yasushi Ikarashi, Hiroyuki Kobayashi","doi":"10.3390/biology14020202","DOIUrl":"10.3390/biology14020202","url":null,"abstract":"<p><p><i>Centella asiatica</i>, a traditional medicinal plant, possesses potent antioxidant activity and may therefore prevent cellular aging and exert antiapoptotic effects. However, these effects remain to be fully elucidated. This study aimed to investigate the protective effects of <i>C. asiatica</i> extract against cellular senescence and apoptosis caused by hydrogen peroxide (H₂O₂)-induced oxidative stress in human epidermal keratinocytes (HaCaT cells). To evaluate the effects of H₂O₂ and <i>C. asiatica</i> on HaCaT cells, we measured cell viability as a marker of cell death; reactive oxygen species (ROS), radical scavenging, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase activities as markers of oxidative stress; senescence-associated β-galactosidase (SA-β-gal) activity as a marker of cellular senescence; and caspase-3/9 activities and apoptotic cells as markers of apoptosis. H₂O₂ induced cell death (decreased cell viability), oxidative stress (increased ROS activity, decreased radical scavenging, SOD, GPx, and catalase activities), cellular senescence (increased SA-β-gal activity), and apoptosis (increased early/late apoptotic cells and increased caspase-3/9 activities). <i>C. asiatica</i> significantly decreased all markers of H₂O₂-induced cell death, oxidative stress, cellular senescence, and apoptosis, suggesting its ability to prevent cellular senescence and apoptosis through its antioxidant activity. This mechanism of action may contribute to the prevention and improvement of skin aging.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Distribution of Microplastic Pollution and Ecological Risk Assessment of Jingpo Lake-The World's Second Largest High-Mountain Barrier Lake.","authors":"Haitao Wang, Chen Zhao, Tangbin Huo","doi":"10.3390/biology14020201","DOIUrl":"10.3390/biology14020201","url":null,"abstract":"&lt;p&gt;&lt;p&gt;To investigate the influence of factors such as tourism, agriculture, and population density on the presence of microplastic (MP) content in aquatic environments and their associated ecological risks, Jingpo Lake, a remote high-mountain lake situated away from urban areas, was selected as the research subject. This study examined the abundance, types, sizes, colors, and polymer compositions of MPs within the water body, fish, and sediments. By considering variables, including fishing practices, agricultural activities, population dynamics, and vegetation cover, an analysis was conducted to unravel the spatial and temporal distribution of MPs concerning human activities, ultimately leading to an assessment of the ecological risks posed by MP pollution. The findings revealed that the average abundance of MPs in the lake's surface water was recorded as (304.8 ± 170.5) n/m&lt;sup&gt;3&lt;/sup&gt;, while in the sediments, it averaged (162.0 ± 57.45) n/kg. Inside the digestive tracts of fish, the MP abundance was measured at 11.4 ± 5.4 n/ind. The contamination of MPs within the aquatic environment of Jingpo Lake was found to be relatively minimal. Variations in MP loads across time and space were observed, with MPs predominantly falling within the size range of small planktonic organisms (50-1000 μm). Additionally, the prevalent colors of MPs in the water samples were white or transparent, constituting approximately 55.65% of the entire MP composition. Subsequently, they were black, red, and blue. This colors distribution were consistent across MPs extracted from fish and sediment samples. The chemical compositions of the MPs predominantly comprised PE (31.83%) and PS (25.48%), followed by PP (17.56%), PA (11.84%), PET (6.71%), EVA (4.56%), and PC (2.03%). Regarding the seasonal aspect, MP concentrations were highest during summer (46.68%), followed by spring (36.75%) and autumn (16.56%). The spatial distribution of MPs within Jingpo Lake's water body, fish, and sediments was notably influenced by human activities, as confirmed by Pearson correlation coefficients. A strong association was observed between MP levels and water quality indicators such as ammonium nitrogen (NH&lt;sub&gt;4&lt;/sub&gt;-N), total phosphorus (TP), and chlorophyll-a (Chla), suggesting that human-related pollution contributed significantly to MP contamination. The diversity assessment of MP pollutants exhibited the highest variability in chemical composition (1.23 to 1.79) using the Shannon-Wiener Index. Subsequently, the diversity of colors ranged from 0.59 to 1.54, shape diversity from 0.78 to 1.30, seasonal diversity from 0.83 to 1.10, and size diversity from 0.44 to 1.01. The assessment results of ecological risk highlighted that the risk categories for MPs within the surface water, fish, and sediments of Jingpo Lake were categorized as I for the PHI and PLI and as \"Minor\" for the PERI. These relatively low-risk values were attributed to the predominantly low toxicity of the distributed MPs w","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Quantification Method for Gene-Edited Animal Detection Based on ddPCR.","authors":"Kaili Wang, Yi Ji, Cheng Peng, Xiaofu Wang, Lei Yang, Hangzhen Lan, Junfeng Xu, Xiaoyun Chen","doi":"10.3390/biology14020203","DOIUrl":"10.3390/biology14020203","url":null,"abstract":"<p><p>As gene-editing technologies continue to evolve, gene-edited products are making significant strides. These products have already been commercialized in the United States and Japan, prompting global attention to their safety and regulatory oversight. However, the detection of gene editing still relies on qPCR, and there is a lack of quantitative detection methods to quantify gene-editing components in products. To ensure consumer safety and transparency, we developed a novel droplet digital PCR (ddPCR)-based detection method for gene-edited products. Primers and probes were designed targeting the editing sites of MSTN-edited cattle, and the method was evaluated for specificity, sensitivity, real sample testing, and detection thresholds. Our results demonstrate that this ddPCR method is highly specific, with a detection limit of 5 copies/µL, and it successfully detected MSTN edits in all 11 tested samples. Tests using both actual gene-edited cattle samples and plasmid DNA at concentrations of 5%, 1%, and 0.01% yielded consistent results, indicating the method's suitability for real-world applications. This ddPCR assay provides a sensitive and specific tool for detecting MSTN gene-edited cattle and determining the presence of gene-edited products, offering crucial support for regulatory monitoring of gene-edited animal-derived foods.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11852154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host Specificity of the Bioherbicidal Fungal Strain <i>Paramyrothecium eichhorniae</i> TBRC10637 for Control of Water Hyacinth.","authors":"Tanyapon Siriphan, Arm Unartngam, Wachiraya Imsabai, Piyangkun Lueangjaroenkit, Chatchai Kosawang, Hans Jørgen Lyngs Jørgensen, Jintana Unartngam","doi":"10.3390/biology14020199","DOIUrl":"10.3390/biology14020199","url":null,"abstract":"<p><p><i>Paramyrothecium eichhorniae</i> TBRC10637 has been reported as a potential biocontrol agent of water hyacinth (<i>Eichhornia crassipes</i>) in Thailand. Despite its great potential, it remained unclear whether the strain may cause disease in other plant species, especially those sharing the same niche as water hyacinth. Here, we examined the strain for its specificity and pathogenicity on 55 plant species from 26 families ranging from crop plants to aquatic weeds. We showed that, except for water hyacinth, <i>P. eichhorniae</i> TBRC10637 did not cause leaf spot or leaf blight or on any of the tested plants. Scanning electron microscopy of spores inoculated on eight plant species, including economically important plants such as maize (<i>Zea mays</i>) and chilli (<i>Capsicum annuum</i>) at 0, 24, 48, and 72 h after inoculation, showed no spore germination, except on water hyacinth. Inoculation with spore-free culture washing led to blight symptoms on leaves of water hyacinth 72 h after inoculation, suggesting that enzymes and secondary metabolites may be involved in causing the blight symptoms. Our results confirmed high specificity of <i>P. eichhorniae</i> TBRC10637 towards water hyacinth, paving the way to control the spread of water hyacinth effectively.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143505158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}