Bioimpacts最新文献

{"title":"Protective effects of sumatriptan against optic nerve injury in rats via modulation of kynurenine pathway, oxidative stress and apoptosis.","authors":"Moein Ala, Razieh Mohammad Jafari, Leila Aghajanpour, Mehdi Sanatkar, Masoud Aghsaei Fard, Sepideh Goudarzi, Amir Shadboorestan, Ahmad Reza Dehpour","doi":"10.34172/bi.30409","DOIUrl":"https://doi.org/10.34172/bi.30409","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Traumatic optic neuropathy (TON) is an acute visual dysfunction subsequent to head and neck trauma. Despite immense efforts, there is no effective treatment to minimize the damage caused by TON. Due to its anti-inflammatory and neuroprotective properties, we aimed to measure the effect of sumatriptan on optic nerve injury in rats.</p><p><strong>Methods: </strong>Bulldog forceps were used to induce optic nerve crush. Immediately after trauma, a single dose of sumatriptan was intravitreally injected and rats were just observed for 1 week. Visual evoked potential (VEP) was recorded to assess optic nerve function on days 2, 5, and 7 after optic nerve injury. Retinas were extracted seven days after trauma to assess molecular and microscopic changes.</p><p><strong>Results: </strong>Crushing force reduced cell survival, decreased the amplitude of the waves, and prolonged their latency in VEP. In contrast, sumatriptan significantly increased cell survival and shortened the latency of P2 and N2 waves. Likewise, sumatriptan significantly decreased the tissue levels of toll-like receptor 4 (TLR4), phosphorylated extracellular signal-regulated kinase (p-ERK), malondialdehyde (MDA), indole-amine 2,3-dioxygenase 1 (IDO), tumor necrosis factor α (TNF-α), interferon γ (INF-γ), and kynurenine in the retinas of rats.</p><p><strong>Conclusion: </strong>These findings suggest that sumatriptan can enhance retinal cell viability, improve optic nerve function, and decrease inflammation, possibly through attenuation of TLR4, ERK, and kynurenine signaling pathways. Thus, future clinical trials should assess the efficacy of low-dose intravitreal sumatriptan for patients with TON.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30409"},"PeriodicalIF":2.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimuli-responsive synthesis of silver nanoparticles applying green and chemical reduction approaches.","authors":"Seraj Mohaghegh, Karim Osouli-Bostanabad, Hossein Nazemiyeh, Yadollah Omidi, Hossein Maleki-Ghaleh, Mohammad Barzegar-Jalali","doi":"10.34172/bi.30286","DOIUrl":"10.34172/bi.30286","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>The current study reports the comparative stimuli-responsive synthesis of silver nanoparticles (AgNPs) with various sizes and morphologies employing <i>Lycium ruthenicum</i> extract and sodium citrate solutions.</p><p><strong>Methods: </strong>The morphology and size of AgNPs were regulated by varying the pH values, concentrations of the extract solution, and temperatures in the reaction medium. The prepared AgNPs were assessed via various instrumental analyses, including UV-Vis, FTIR, XRD, TEM, and DLS.</p><p><strong>Results: </strong>The <i>L. ruthenicum</i> extract displayed several functional groups that reduced the Ag ions to the AgNPs at different values of pH. However, the primary chemical structure of <i>L. ruthenicum</i> was virtually unaltered at these conditions. Variations in the pH and extract concentration of the reaction medium yielded AgNPs of different sizes and morphologies. Both bio- and chemo-synthesized AgNPs revealed a relatively dispersed sphere-shaped morphology under alkaline conditions (≈ 36 nm).</p><p><strong>Conclusion: </strong>This study introduced a simple, valuable, and green technique for stimuli-sensitive AgNPs synthesis employing the <i>L. ruthenicum</i> extract.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30286"},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kopexil vs minoxidil: <i>In vivo</i> comparative study on hair growth, hair growth promoting factors and toxicity.","authors":"Sina Jalilzadeh, Hamed Hamishehkar, Farnaz Monajjemzadeh","doi":"10.34172/bi.30484","DOIUrl":"https://doi.org/10.34172/bi.30484","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>This study aimed to investigate the impact of a hydro-alcoholic solution containing kopexil on hair growth, and growth factors, and to evaluate its skin penetration, as well as its potential toxic effects on the liver and kidneys in animal models.</p><p><strong>Methods: </strong>Animal studies were conducted on mice over 28 days, involving minoxidil (positive control), kopexil (test), and negative control groups. Morphological characteristics of skin and hair were assessed. Levels of hair growth-promoting markers (HGF and VEGF) were determined through western blot analysis. Toxic effects were examined by isolating and weighing the kidneys and livers, followed by histological examination.</p><p><strong>Results: </strong>The kopexil group demonstrated significant increases in hair weight, follicle count, percentage of anagen hair, and hair growth compared to the minoxidil. Western blot analysis revealed higher expression levels of hair growth-promoting factors in the kopexil-treated group. No statistically significant differences in liver and kidney weights or noticeable morphological variations were observed in the toxicity tests across the groups.</p><p><strong>Conclusion: </strong>The 5% (w/v) hydro-alcoholic solution containing kopexil proved to be an effective hair growth stimulator, influencing various factors. Its daily use can be considered a suitable treatment method for stimulating hair growth, given its improved effectiveness and ease of use for patients.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30484"},"PeriodicalIF":2.2,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The treatment of psoriasis via herbal formulation and nano-polyherbal formulation: A new approach.","authors":"Tejpal Yadav, Hemant Kumar Singh Yadav, Abhay Raizaday, Md Sabir Alam","doi":"10.34172/bi.30341","DOIUrl":"https://doi.org/10.34172/bi.30341","url":null,"abstract":"<p><p>Psoriasis is a chronic condition that can strike at any age. This sickness is associated with inflammatory problems that impact all humans in the world. Psoriasis is more common in Scandinavians than in Asian and African populations due to a combination of factors such as age, gender, geographic location, ethnicity, genetic and environmental factors. Immune stimulation, genetic contribution, antimicrobial peptides, and other significant triggers such as medicines, immunizations, infections, trauma, stress, obesity, alcohol intake, smoking, air pollution, sun exposure, and particular disorders cause psoriasis. Numerous clinical research investigations are now underway, and therapeutic alternatives are available. However, these therapies only improve symptoms and do not accomplish a complete cure; they also have dangerous and undesirable side effects. Natural products have gained popularity recently due to their great effectiveness, safety, and low toxicity. Natural formulations of various nanocarriers like liposomes, lipospheres, nanogels, emulgel, nanostructured lipid carriers, nanosponge, nanofibers, niosomes, nanomiemgel, nanoemulsions, nanospheres, cubosomes, microneedles, nanomicelles, ethosomes, nanocrystals, and foams, have significantly contributed and encouraged advancement in psoriasis disease treatment. These phytochemical-loaded new nanoformulations address several issues associated with natural products in conventional dosage forms, such as instability, poor solubility, and limited bioavailability. This article reviews some of the intriguing phytochemicals, as well as their possible molecular target locations and mechanisms of action, which may assist in the development of more specific and selective antipsoriatic medicines. Exploring and understanding phytochemicals' functions will allow for more site-specific psoriasis treatment techniques. This review concluded the psoriasis disease with phytoconstituent loaded herbal or polyherbal nanocarriers and their mechanistic approach.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30341"},"PeriodicalIF":2.2,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review on the use of phytotherapy in managing clinical depression.","authors":"Sutude Fazilat, Fateme Tahmasbi, Mohammad Reza Mirzaei, Sarvin Sanaie, Zahra Yousefi, Solmaz Asnaashari, Shadi Yaqoubi, Ahad Banagozar Mohammadi, Mostafa Araj-Khodaei","doi":"10.34172/bi.30532","DOIUrl":"https://doi.org/10.34172/bi.30532","url":null,"abstract":"<p><strong>Introduction: </strong>The use of medicinal plants in the management of depression, also known as phytotherapy or herbal medicine for depression, is an area of growing interest in the field of mental health and complementary medicine.</p><p><strong>Methods: </strong>This study used a systematic assessment of pertinent literature to assess the effectiveness of medicinal herbs in treating mild to severe depression. A comprehensive literature search was conducted to identify randomized controlled trials (RCTs) that reported data on the intervention, control group, adverse events, outcome measurements, and main findings. A summary and analysis were done on the included research data.</p><p><strong>Results: </strong>We included 23 RCTs investigating the efficacy of herbal medicines, including <i>Crocus sativus, Lavandula angustifolia, Melissa officinalis,</i> and <i>Echium amoenum</i>, in treating depression. In general, saffron showed encouraging outcomes when used to treat mild to severe depression. With no discernible variations in the reported adverse effects, it proved to be equally efficacious as well-known antidepressants like imipramine and fluoxetine. However, it is noteworthy that not all trials yielded favorable results.</p><p><strong>Conclusion: </strong>More investigation is required to fully understand the mechanisms of action, ideal dosage schedules, long-term effects, and relative efficacy of medicinal plants in depressive treatment.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30532"},"PeriodicalIF":2.2,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the role of classical oestrogen receptor in insulin resistance and type 2 diabetes mellitus: From molecular mechanism to clinical evidence.","authors":"Haryati Ahmad Hairi, Nurul Izzah Ibrahim, Muhammad Zulfiqah Sadikan, Putri Ayu Jayusman, Ahmad Nazrun Shuid","doi":"10.34172/bi.30378","DOIUrl":"https://doi.org/10.34172/bi.30378","url":null,"abstract":"<p><p>The biological actions of oestrogen are mediated by the oestrogen receptor α or β (ERα or ERβ), which are members of a broad nuclear receptor superfamily. Numerous <i>in vivo</i> and <i>in vitro</i> studies have demonstrated that loss of circulating oestrogen modulated by classical ERα and ERβ led to rapid changes in pancreatic β-cell and islet function, GLUT4 expression, insulin sensitivity and glucose tolerance, dysfunctional lipid homeostasis, oxidative stress, and inflammatory cascades. Remarkably, 17β-oestradiol (E2) can completely reverse these effects. This review evaluates the current understanding of the protective role of classical ER in critical pathways and molecular mechanisms associated with insulin resistance and type 2 diabetes mellitus (T2DM). It also examines the effectiveness of menopausal hormone therapy (MHT) in reducing the risk of developing T2DM in menopausal women. Clinical trials have shown the protective effects of MHT on glucose metabolism, which may be useful to treat T2DM in perimenopausal women. However, there are concerns about E2's potential side effects of obesity and hyperlipidaemia in menopausal women. Further studies are warranted to gain understanding and find other oestrogen alternatives for treatment of insulin resistance and T2DM in postmenopausal women.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30378"},"PeriodicalIF":2.2,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained release microneedle patch for pronounced systemic delivery of doxazosin mesylate.","authors":"Imran Anwar, Nadiah Zafar, Asif Mahmood, Zulcaif, Riffat Latif","doi":"10.34172/bi.30257","DOIUrl":"10.34172/bi.30257","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Microneedle patch is one of the fascinating drug delivery approaches that offers low invasiveness and a painless physical application to enhance the delivery of micro and macro-molecules into the skin.</p><p><strong>Methods: </strong>Variable contents of chitosan and polyvinyl alcohol were used for the development of doxazosin mesylate containing sustained release microneedle patches via solvent casting technique. The prepared patches were evaluated for microscopic evaluation, mechanical strength, drug loading (%) and Fourier transform infrared spectroscopy (FTIR) etc. The skin penetration study was performed by using pig ear skin and results were captured through confocal microscopy. <i>Ex-vivo</i> release study and pharmacokinetic evaluation were also performed.</p><p><strong>Results: </strong>Sharp needle tips with a height of 600µm and a base of 200µm were confirmed through microscopic examination. Optimized formulation (SRF-6) exhibited loading of 92.11% doxazosin mesylate with appreciable strength up to 1.94N force. <i>Ex-vivo</i> release studies revealed 87.24% release within 48 hours. Moreover, the pharmacokinetic parameters in case of optimized patch formulation (SRF-6) were markedly improved i.e. MRT (19.46 h), AUC (57.12 μg.h /mL), C_max (2.16 µg /mL), t_max (10.10 h) and t_1/2 (6.32 h) as compared to commercially available tablet. Biocompatibility of the developed patches was validated from skin irritation studies.</p><p><strong>Conclusion: </strong>Results confirmed the successful fabrication of microneedle patch having sufficient strength and effective penetration ability into the skin to ensure controlled release of incorporated drug for the intended duration. It can be employed as an efficient carrier system for other therapeutics those are prone to bioavailability issues due to first pass effect after their oral administration.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30257"},"PeriodicalIF":2.2,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular dynamics simulation in tissue engineering.","authors":"Ali Rahmani, Rahim Jafari, Samad Nadri","doi":"10.34172/bi.30160","DOIUrl":"10.34172/bi.30160","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>In tissue engineering, the interaction among three primary elements, namely cells, material scaffolds, and stimuli, plays a pivotal role in determining the fate of cells and the formation of new tissue. Understanding the characteristics of these components and their interplay through various methodologies can significantly enhance the efficiency of the designed tissue engineering system. In silico methods, such as molecular dynamics (MD) simulation, use mathematical calculations to investigate molecular properties and can overcome the limitations of laboratory methods in delivering adequate molecular-level information.</p><p><strong>Methods: </strong>The studies that used molecular dynamics simulation, either alone or in combination with other techniques, have been reviewed in this paper.</p><p><strong>Results: </strong>The review explores the use of molecular dynamics simulations in studying substrate formation mechanism and its optimization. It highlights MD simulations' role in predicting biomolecule binding strength, understanding substrate properties' impact on biological activity, and factors influencing cell attachment and proliferation. Despite limited studies, MD simulations are considered a reliable tool for identifying ideal substrates for cell proliferation. The review also touches on MD simulations' contribution to cell differentiation studies, emphasizing their role in designing engineered extracellular matrix for desired cell fates.</p><p><strong>Conclusion: </strong>Molecular dynamics simulation as a non-laboratory tool has many capabilities in providing basic and practical information about the behavior of the molecular components of the cell as well as the interaction of the cell and its components with the surrounding environment. Using this information along with other information obtained from laboratory tools can ultimately lead to the advancement of tissue engineering through the development of more appropriate and efficient methods.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30160"},"PeriodicalIF":2.2,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot hydrothermal synthesize and characterization of Au/Gd bimetallic nanostructure as potential contrast agents in CT and MR imaging.","authors":"Rashed Ahmadyani, Hamed Ahmadyani, Milad Moradi, Sajad Moradi, Mohsen Shahlaei, Saleh Salehi Zahabi, Faranak Aghaz, Kave Moloudi, Negin Farhadian","doi":"10.34172/bi.30459","DOIUrl":"https://doi.org/10.34172/bi.30459","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Multimodal contrast agents play an important role in early diagnosis of diseases and monitoring the treatment outcomes by increasing the accuracy and clarity of images.</p><p><strong>Methods: </strong>Herein, a one-step simple hydrothermal route is utilized to prepare the gadolinium-1,4 H₂BDC- metal-organic framework (Gd-BDC-MOF) nanoparticles decorated with gold nanoclusters. The physicochemical properties of bimetal nanostructures were evaluated, their safety and ability to enhance contrast in both CT and MR imaging were also examined.</p><p><strong>Results: </strong>The spherical nano-sized Au decorated Gd bimetal nanostructures have an average diameter of 64 nm. The presence of both Au and Gd metals in the prepared nanostructure was confirmed using EDAX. The XRD pattern shows that, in the hydrothermal synthesis of two metals using a terephthalic acid linker, gold nanoclusters are decorated onto the gadolinium metal organic framework. The FTIR analysis confirmed the attachment of Gd to the carboxylate group of the organic linker. The bimetal nanostructure sample with a concentration of 40 mM showed similar X-ray attenuation to that of iodine at a concentration of 128 mM. At a magnetic field strength of 1.5 T, the longitudinal relaxivity value of the bimetal nanostructure was determined to be 13.635 mM^-1 s^-1. The MTT assay demonstrated the cytocompatibility and safety of the contrast agent synthesized for biomedical applications.</p><p><strong>Conclusion: </strong>The fabricated bimetal nanostructure exhibits dose-dependent positive contrast enhancement in both MR and CT imaging techniques, making it a promising candidate for use as a contrast agent in medical applications.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30459"},"PeriodicalIF":2.2,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lactiplantibacillus plantarum</i> exerts anticancer effects and increase the chemosensitivity of 5-fluorouracil against oral cancer cells <i>in vitro</i>.","authors":"Fathima Fida, Subramaniyan Yuvarajan, Kesari Ashwath, Punchappady Devasya Rekha","doi":"10.34172/bi.30427","DOIUrl":"https://doi.org/10.34172/bi.30427","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Probiotics are used to provide health benefits and can improve the immune response. They can also target cancer cells directly with anticancer effects through various mechanisms. In this study, <i>Lactiplantibacillus plantarum</i> (basonym: <i>Lactobacillus plantarum</i>) strain MCC 3016 and its postbiotic metabolites/cell free supernatant (CFS) were used against Cal27 oral cancer cells <i>in vitro</i>.</p><p><strong>Methods: </strong>Standard assays were employed to investigate the effect of <i>Lpb. plantarum</i> on cell viability, proliferation, migration, and clonogenicity of Cal27 cells. The mechanism of action was assessed by measuring the levels of reactive oxygen species (ROS), interleukins (IL)-6 and IL-8, tumor necrosis factor-α (TNF-α), as well as the expression of Ki67, vascular endothelial growth factor (VEGF), p53 and caspase-3. Further, the effect of <i>Lpb. plantarum</i> and its CFS on the cytotoxicity of chemotherapy drug 5-fluorouracil (5-FU) was evaluated using cell viability assays.</p><p><strong>Results: </strong>Cal27 cells treated with <i>Lpb. plantarum</i> and its CFS showed a significant decrease (<i>P</i> < 0.01) in cell viability, proliferation, migration, and clonogenicity, along with increased levels of ROS and induced apoptosis. It significantly reduced IL-6, IL-8, TNF-α, and VEGF levels and upregulated p53 and caspase-3 expression. The postbiotic metabolites also showed similar effects on Cal27 cells. Furthermore, the cytotoxic effect of 5-FU on Cal27 cells was enhanced by <i>Lpb. plantarum</i> and its CFS treatment.</p><p><strong>Conclusion: </strong><i>Lpb. plantarum</i> MCC 3016 and its postbiotic metabolites exhibited promising anticancer effects on oral cancer cells and improved drug efficacy, demonstrating their potential therapeutic value in oral cancer therapy.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30427"},"PeriodicalIF":2.2,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}