Bioimpacts最新文献

{"title":"Anti-Alzheimer effects of the newly synthesized cationic compounds as multi-target dual hAChE/hBuChE inhibitor: An <i>in silico</i>, <i>in vitro</i>, and <i>in vivo</i> approach.","authors":"Hosna Karami, Somaieh Soltani, Gerhard Wolber, Saeed Sadigh-Eteghad, Roghaye Nikbakht, Hanieh Farrokhi, Farzaneh Narimani, Reza Teimuri-Mofrad, Mohammad-Reza Rashidi","doi":"10.34172/bi.24196","DOIUrl":"10.34172/bi.24196","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Multi-target anti-Alzheimer's disease (AD) compounds are promising leads for the development of AD modifying agents. Ionic compounds containing quaternary ammonium moiety were synthesized, and their multi-targeted anti-AD effects were examined.</p><p><strong>Methods: </strong>Imidazole derivatives containing a quaternary ammonium moiety were synthesized and evaluated for their potential anti-Alzheimer properties using computational (<i>in silico</i>), cellular (<i>in vitro</i>), and animal (<i>in vivo</i>) models. The inhibition kinetics of both human acetylcholinesterase (hAChE) and butyrylcholinesterase (hBuChE) were assessed. Neuroprotective effects in amyloid-beta (Aβ)-exposed PC12 cells were also examined. Furthermore, the compounds' impact on Aβ-induced memory impairment in Wistar rats was evaluated, with a detailed analysis of the underlying mechanisms.</p><p><strong>Results: </strong>Compound 5g demonstrated acceptable cytotoxicity against human cells. This compound exhibited non-competitive dual inhibition of both hAChE and hBuChE. Additionally, compound 5g mitigated the morphological changes induced by amyloid-beta (Aβ) in PC12 cells and decreased cell mortality. It exhibited anti-oxidative stress properties, evident by reduction in reactive oxygen species (ROS) production, and inhibition of lipid peroxidation. The compound also down regulated the expression of pro-inflammatory genes IL-1β and TNF-α. In vitro studies validated compound 5g's ability to inhibit lactate dehydrogenase (LDH), attenuate neuroinflammation, and prevent the autophagy-apoptosis cascade. When administered to rats with Aβ-induced memory dysfunction, compound 5g enhanced cognitive function and improved spatial memory. In the hippocampi of treated rats, there was a noted downregulation of TNF-α and NF-kB. Furthermore, compound 5g counteracted the elevated activity of AChE. Molecular modeling validated the binding of compound 5g to both steric and catalytic sites of cholinesterase enzymes.</p><p><strong>Conclusion: </strong>The novel quaternary ammonium derivative, compound 5g, demonstrated multi-target anti-AD properties, as evidenced by <i>in silico, in vitro</i> and <i>in vivo</i> studies. Behavioral assessments and molecular analyses further confirmed its therapeutic efficacy in amyloid-beta (Aβ)-challenged rats.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"24196"},"PeriodicalIF":2.2,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and characterization of nanocomposite scaffold containing zinc-doped mesoporous bioglass: Evaluation of the antioxidant properties, hemocompatibility and proliferation of apical papilla stem cells.","authors":"Morteza Jalilvand, Elham Khoshbin, Zahra Barabadi, Hamed Karkehabadi, Esmaeel Sharifi","doi":"10.34172/bi.30300","DOIUrl":"10.34172/bi.30300","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Nanocomposite scaffolds comprising mesoporous bioactive glass (MBG) were able to increase the viability, proliferation, and growth of stem cells in vitro, rendering them promising candidates for dental root tissue regeneration.</p><p><strong>Methods: </strong>The Sol-Gel process was utilized for the synthesis of MBG and zinc-doped MBG (Zn-MBG), the latter being integrated into alginate/chitosan scaffolds which in turn were cross-linked to strengthen mechanical properties, followed by freeze-drying. The scaffold's physicochemical characterizations were evaluated, followed by investigations of its antioxidant properties, swelling behavior, mechanical properties, and porosity. The capacity of these biomaterials to increase cell viability and growth of apical papilla stem cells (SCAPs) and hemocompatibility was assessed as a final step.</p><p><strong>Results: </strong>All fabricated scaffolds demonstrated proper porosity, biocompatibility, and hemocompatibility. Nanocomposite scaffolds with Zn-MBG presented a significant enhancement in cell viability for SCAPs compared to alginate/chitosan scaffolds. DPPH tests indicated that the Zn-MBG-alginate/chitosan scaffold showed the highest antioxidant properties.</p><p><strong>Conclusion: </strong>Zn-MBG-alginate/chitosan nanocomposite scaffolds demonstrated great physicochemical characteristics and biological and mechanical properties, marking them as suitable candidates for dental root tissue engineering.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30300"},"PeriodicalIF":2.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondrogenic potential of PMSCs cultured on chondroitin sulfate/gelatin-modified DBM scaffold.","authors":"Fatemeh Haghwerdi, Ismaeil Haririan, Masoud Soleimani","doi":"10.34172/bi.2023.30003","DOIUrl":"10.34172/bi.2023.30003","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Osteoarthritis is one of the most common orthopedic diseases that gradually causes wear and damage to the articular Subchondral bone due to the destruction of articular cartilage. One of the basic challenges in cartilage tissue engineering is the choice of scaffold. In the design of the cartilage scaffold, it is useful to consider parameters such as porosity, water absorption, high mechanical resistance, biocompatibility, and biodegradability. Therefore, in this study, demineralized bone matrix (DBM), which inherently has these characteristics to some extent, was chosen as the basic scaffold.</p><p><strong>Methods: </strong>The gelatin/DBM (G/DBM) and the chondroitin sulfate-gelatin/DBM (GCS/DBM) scaffolds were prepared, respectively, by incorporating gelatin or chondroitin sulfate/gelatin solution inside DBM pores, freeze-drying and crosslinking with EDC/NHS. The physicochemical, biological characteristics and chondrogenic potential of scaffolds were studied.</p><p><strong>Results: </strong>According to the SEM results, the size of the DBM pores in the G/DBM and GCS/DBM scaffolds decreased (from almost 100-1500 µm to less than 200 µm), which reduced cell escape compared to the DBM scaffold. Also, crosslinking the scaffolds has greatly increased their compressive E-modulus (more than 8 times). The cytocompatibility and non- toxicity of all scaffolds were confirmed by acridine orange/ethidium bromide (AO/EB) staining. The evaluation results of chondrogenic differentiation of placenta-derived mesenchymal stem cells (PMSCs) on modified scaffolds, using the real-time PCR method, showed that the presence of CS in the GCS/DBM scaffold improved the expression of chondrogenesis markers such as Aggrecan (AGC) (~4 times) and collagen 2 (COL-2) (~2.2 times) compared to the DBM scaffold. Also, Alcian blue staining and immunohistochemical analyses of the scaffolds showed denser and more coherent GAGs and COL-2 protein synthesis on the GCS/DBM than the G/DBM and DBM scaffolds.</p><p><strong>Conclusion: </strong>According to the results, the GCS/DBM scaffold can be a suitable scaffold for cartilage tissue engineering.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30003"},"PeriodicalIF":2.2,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug self-delivery systems: A comprehensive review on small molecule nanodrugs.","authors":"Mahsa Sayed Tabatabaei, Fakhredin A Sayed Tabatabaei, Hamid Reza Moghimi","doi":"10.34172/bi.30161","DOIUrl":"10.34172/bi.30161","url":null,"abstract":"<p><p>Drug self-delivery systems are nanostructures composed of a drug as the main structural unit, having the ability of intracellular trafficking with no additional carrier. In these systems, the drug itself undertakes the functional and structural roles; thereby, the ancillary role of excipients and carrier-related limitations are circumvented and therapeutic effect is achieved at a much lower dose. Such advantages -which are mainly but not exclusively beneficial in cancer treatment- have recently led to an upsurge of research on these systems. Subsequently, various terminologies were utilized to describe them, referring to the same concept with different words. However, not all the systems developed based on the self-delivery approach are introduced using one of these keywords. Using a scoping strategy, this review aims to encompass the systems that have been developed as yet -inspired by the concept of self-delivery- and classify them in a coherent taxonomy. Two main groups are introduced based on the type of building blocks: small molecule-based nanomedicines and self-assembling hybrid prodrugs. Due to the diversity, covering the whole gamut of topics is beyond the scope of a single article, and, inevitably, the latter is just briefly introduced here, whereas the features of the former group are meticulously presented. Depending on whether the drug is merely a carrier for itself or carries a second drug as cargo, two classes of small molecule-based nanomedicines are defined (i.e., pure nanodrugs and carrier-mimicking systems, respectively), each having sub-branches. After introducing each branch and giving some examples, possible strategies for designing each particular system are visually displayed. The resultant mind map can create a macro view of the taken path and its prospects, give a profound insight into opportunities, spark new ideas, and facilitate overcoming obstacles. Taken together, one can foresee a brilliant future for self-delivery systems as a pioneering candidate for the next generation of drug delivery systems.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30161"},"PeriodicalIF":2.2,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An update on technical method of cartilage decellularization: A physical-based protocol.","authors":"Hengameh Dortaj, Ahmad Vaez, Ashraf Hassanpour-Dehnavie, Ali Akbar Alizadeh","doi":"10.34172/bi.2024.30047","DOIUrl":"10.34172/bi.2024.30047","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Despite advances in orthopedic surgery, the lack of effective conventional treatment for cartilage defects has led to research in cartilage tissue engineering. One of the interesting topics is the use of decellularized extracellular matrix (ECM) as a suitable natural scaffold that supports the growth and function of cells cultured in it. A concern with decellularization protocols, especially those with high detergent concentrations, is the disruption of native ECM, which has deleterious effects on subsequent scaffold recellularization. Therefore, this study focused on optimizing cartilage decellularization by physical methods without the use of ionic detergents.</p><p><strong>Methods: </strong>The bovine tracheal cartilage fragments were decellularized by a combination of 8 cycles of freeze-thaw and ultrasound techniques. Then, the tissues were immersed and shaken in 0.25% trypsin for 24 hours. Efficient cell removal and preservation of ECM were confirmed by histological and cytocompatibility assessments. The in-vivo studies were performed to evaluate the biocompatibility and bioactivity of the scaffold.</p><p><strong>Results: </strong>The histological assessments indicated the appropriate cytocompatibility and the fibroblast cell culture study demonstrated that cells were able to proliferate and migrate on the decellularized cartilage. In-vivo evaluation also showed a reduced adverse immune response, including leukocyte infiltration into the ECM.</p><p><strong>Conclusion: </strong>These results suggest that a cartilage scaffold created using a physical decellularization protocol that efficiently removes cells while preserving the native ECM can be a suitable scaffold for cartilage reconstruction. The main advantage of this protocol is the absence of potentially toxic chemicals in the tissues.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30047"},"PeriodicalIF":2.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the function and targeting of MET protein as an oncogene kinase in pancreatic ductal adenocarcinoma: A microarray data integration.","authors":"Nahid Askari, Morteza Hadizadeh, Mohammad Sina, Sepideh Parvizpour, Seyedeh Zahra Mousavi, Mohd Shahir Shamsir","doi":"10.34172/bi.30187","DOIUrl":"10.34172/bi.30187","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal disease with a poor prognosis. Kinase proteins are essential regulators of cellular processes and potential targets for drug development.</p><p><strong>Methods: </strong>Integration of multiple microarray datasets was screened to find differentially expressed kinases (DE-Kinases) across adjacent normal and tumor tissue samples in PDAC. The most effective kinase for drug design and docking in this study was selected by investigating biological mechanisms and survival analyses. Forty phytochemicals were extracted from the yellow sweet clover, <i>Melilotus officinalis</i> (Linn.) Pall, and were then subjected to in silico screening and molecular docking studies against a specific potent kinase.</p><p><strong>Results: </strong>MET, PAK3, and PDK4 were identified as the DE-Kinases. After examining the pathways and biological processes, up-regulated MET had the most significant survival analysis and became our primary kinase for drug design and docking in this study. Four of the extracted phytocompounds of <i>Melilotus officinalis</i> (Linn.) Pall that exhibited high binding affinities with MET and were selected for toxicity analysis. Finally, the stability and mobility of the two nontoxic compounds that passed the toxicity test (dicumarol PubChem CID: 54676038 and melilotigenin PubChem CID: 14059499) were studied by molecular dynamics simulation.</p><p><strong>Conclusion: </strong>This study's results identified two phytochemicals in yellow sweet clover that could be used to develop an anticancer drug, but experimental evaluation is necessary to confirm their efficacy.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30187"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automatic classification of <i>Giardia</i> infection from stool microscopic images using deep neural networks.","authors":"Pezhman Yarahmadi, Ehsan Ahmadpour, Parham Moradi, Nasser Samadzadehaghdam","doi":"10.34172/bi.30272","DOIUrl":"10.34172/bi.30272","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Giardiasis is a common intestinal infection caused by the <i>Giardia</i> lamblia parasite, and its rapid and accurate diagnosis is crucial for effective treatment. The automatic classification of <i>Giardia</i> infection from stool microscopic images plays a vital role in this diagnosis process. In this study, we applied deep learning-based models to automatically classify stool microscopic images into three categories, namely, normal, cyst, and trophozoite.</p><p><strong>Methods: </strong>Unlike previous studies focused on images acquired from drinking water samples, we specifically targeted stool samples. In this regard, we collected a dataset of 1610 microscopic digital images captured by a smartphone with a resolution of 2340 × 1080 pixels from stool samples under the Nikon YS100 microscope. First, we applied CLAHE (Contrast Limited Adaptive Histogram Equalization) histogram equalization a method to enhance the image quality and contrast. We employed three deep learning models, namely Xception, ResNet-50, and EfficientNet-B0, to evaluate their classification performance. Each model was trained on the dataset of microscopic images and fine-tuned using transfer learning techniques.</p><p><strong>Results: </strong>Among the three deep learning models, EfficientNet-B0 demonstrated superior performance in classifying <i>Giardia</i> lamblia parasites from stool microscopic images. The model achieved precision, accuracy, recall, specificity, and F1-score values of 0.9599, 0.9629, 0.9619, 0.9821, and 0.9607, respectively.</p><p><strong>Conclusion: </strong>The EfficientNet-B0 showed promising results in accurately identifying normal, cyst, and trophozoite forms of <i>Giardia</i> lamblia parasites. This automated classification approach can provide valuable assistance to laboratory science experts and parasitologists in the rapid and accurate diagnosis of giardiasis, ultimately improving patient care and treatment outcomes.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30272"},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of lumateperone and novel anti-psychotics for schizophrenia.","authors":"S Rehan Ahmad, Md Zeyaullah, Abdullah M AlShahrani, Mohammad Suhail Khan, Adam Dawria, Ali Mohieldin, Haroon Ali, Abdelrhman Ag Altijani, Mohammad Shane Alam, Munzila Mehdi, Sabika Akram, Ejaz Rizvi Hussain, Mohammad Amjad Kamal","doi":"10.34172/bi.30259","DOIUrl":"10.34172/bi.30259","url":null,"abstract":"<p><p>Schizophrenia is a devastating chronic mental health illness which includes a complex set of symptoms like hallucination, illusion and delusion, and to manage, lifelong antipsychotic medications are needed. Schizophrenia affects 1% of the population worldwide, and to date, two different classes of antipsychotics, i.e., typical and atypical antipsychotics, are available in the market, and there is an urgent need for promising antipsychotic drugs. In this review, we focus on recently approved antipsychotics and then focus on different antipsychotic drugs under clinical trials. In this review, we first focus on lumateperone in detail, which was approved in December 2019 by the Food and Drug Administration (FDA) and simultaneously modulates serotonin, glutamate and dopamine neurotransmitters and is used at doses of 10.5-, 21- and 42 mg, which show mild adverse effects like constipation, sedation, somnolence and fatigue. This review also focuses on a few more emerging antipsychotics like brexpiprazole, brilaroxazine, roluperidone, F17464, pimavanserin (ACP-103), xanomeline, BI 409306, BI 425809 and MK-8189 which are under different phase of clinical trials and might get approved soon. Brexpiprazole and brilaroxazine act on dopamine receptors, whereas xanomeline, pimavanserin and roluperidone do not act on D2 receptors and manage the symptoms. All the antipsychotic drugs covered did not show any other severe adverse effects except gastrointestinal issues and cardiometabolic risk factors. However, still rigorous clinical trials and modifications are needed to manage adverse effects, and we can expect a few antipsychotics to be on the market soon.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30259"},"PeriodicalIF":2.2,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annexin A1, calreticulin and high mobility group box 1 are elevated in secondary progressive multiple sclerosis: Does immunogenic cell death occur in multiple sclerosis?","authors":"Mohammad Saeid Hejazi, Sevda Jafari, Soheila Montazersaheb, Ommoleila Molavi, Vahid Hosseini, Mahnaz Talebi, Masoud Nikanfar","doi":"10.34172/bi.30264","DOIUrl":"10.34172/bi.30264","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Multiple sclerosis (MS) is a chronic neuroinflammatory diseases characterized by demyelination of the nerve fibers. Immunogenic cell death (ICD) is a process, during which damaged and stressed cells release danger-associated molecular patterns (DAMPs) activating immune responses. This study aimed to elucidate the induction of ICD in MS diseases.</p><p><strong>Methods: </strong>To achieve this goal, the level of DAMPs including Annexin A1 (ANXA1), calreticulin and HMGB1 was measured in the cerebrospinal fluid (CSF) of a secondary progressive multiple sclerosis (SPMS) patient in comparison to control group.</p><p><strong>Results: </strong>Results showed significant upregulation (more than two-fold) of ANXA1, calreticulin (CRT) and HMGB1 in the CSF of the patient.</p><p><strong>Conclusion: </strong>Although further studies are suggested in this regard, this data could imply induction of ICD in MS. The proposed ICD might trigger immune response against neural cells resulting in neuroinflammation and demyelination in CNS in MS. Our observation could suggest inclusion of ICD interfering treatments in routine MS therapy.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30264"},"PeriodicalIF":2.2,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimuli-responsive synthesis of silver nanoparticles applying green and chemical reduction approaches.","authors":"Seraj Mohaghegh, Karim Osouli-Bostanabad, Hossein Nazemiyeh, Yadollah Omidi, Hossein Maleki-Ghaleh, Mohammad Barzegar-Jalali","doi":"10.34172/bi.30286","DOIUrl":"10.34172/bi.30286","url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>The current study reports the comparative stimuli-responsive synthesis of silver nanoparticles (AgNPs) with various sizes and morphologies employing <i>Lycium ruthenicum</i> extract and sodium citrate solutions.</p><p><strong>Methods: </strong>The morphology and size of AgNPs were regulated by varying the pH values, concentrations of the extract solution, and temperatures in the reaction medium. The prepared AgNPs were assessed via various instrumental analyses, including UV-Vis, FTIR, XRD, TEM, and DLS.</p><p><strong>Results: </strong>The <i>L. ruthenicum</i> extract displayed several functional groups that reduced the Ag ions to the AgNPs at different values of pH. However, the primary chemical structure of <i>L. ruthenicum</i> was virtually unaltered at these conditions. Variations in the pH and extract concentration of the reaction medium yielded AgNPs of different sizes and morphologies. Both bio- and chemo-synthesized AgNPs revealed a relatively dispersed sphere-shaped morphology under alkaline conditions (≈ 36 nm).</p><p><strong>Conclusion: </strong>This study introduced a simple, valuable, and green technique for stimuli-sensitive AgNPs synthesis employing the <i>L. ruthenicum</i> extract.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":"15 ","pages":"30286"},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11954753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}