Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology最新文献

{"title":"Alterations of Store-Operated Calcium Entry in Neurodegenerative Pathologies: History, Facts, and Prospects","authors":"V. A. Vigont,&nbsp;E. V. Kaznacheyeva","doi":"10.1134/S1990747824700168","DOIUrl":"10.1134/S1990747824700168","url":null,"abstract":"<p>Neurodegenerative diseases, along with cardiovascular and oncological pathologies, are one of the most acute problems of modern medicine requiring an integrated approach to the study of the molecular mechanisms of their pathogenesis and the search for new targets for the drug treatment. Neuronal calcium signaling deserves close attention of researchers; numerous violations of it have been noted in the study of a number of neurodegenerative pathologies. In this review, we have focused on one of the most common and important ways of calcium influx into the cell, store-operated calcium entry. Here are collected studies demonstrating alterations of the store-operated calcium entry in various neurodegenerative diseases, primarily in Alzheimer’s, Parkinson’s, and Huntington’s diseases, the molecular determinants mediating these disorders are analyzed, and ways of their pharmacological correction are proposed. The information summarized in this review will allow us to look at store-operated channels as one of the most promising targets in the search for new therapeutic agents to treat neurodegenerative pathologies and outline further promising directions of research in this area.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"149 - 159"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-Dependent Reactions of Blood Coagulation: Classical View and State-of-the-Art Concepts","authors":"T. A. Kovalenko,&nbsp;M. A. Panteleev","doi":"10.1134/S199074782470020X","DOIUrl":"10.1134/S199074782470020X","url":null,"abstract":"<p>The complex mechanism called hemostasis evolved in living organisms to prevent blood loss when a blood vessel is damaged. In this process, two closely interconnected systems are distinguished: platelet-vascular and plasmatic hemostasis. Plasmatic hemostasis is a system of proteolytic reactions, in which blood plasma proteins called coagulation factors are involved. A key feature of this system is the localization of enzymatic reactions on the surface of phospholipid membranes, which increases their rate by up to 5 orders of magnitude. This review describes the basic mechanisms of coagulation factors binding to phospholipid membranes, the pathways for complex assembly and activation reactions, and discusses the role of membranes in this process, their composition and sources. The binding of coagulation factors to procoagulant membranes leads not only to the acceleration of coagulation reactions, but also to their selective localization in restricted areas and protection from being washed away by the flow. The efficiency of coagulation reactions is regulated by the composition of the outer layer of the membrane, primarily through a special mechanism of mitochondria-dependent necrotic platelet death.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"200 - 218"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Lipid-Mediated Regulation of the Pore-Forming Activity of Antimicrobial Agents: Studies on Planar Lipid Bilayers","authors":"S. S. Efimova,&nbsp;O. S. Ostroumova","doi":"10.1134/S1990747824700247","DOIUrl":"10.1134/S1990747824700247","url":null,"abstract":"<p>Planar lipid bilayers are unique tools designed for modeling cell membranes and electrophysiological studies of ion channels embedded in them. Such model systems were invented to intentionally limit the complexity and multicomponent nature of cell membranes in order to analyze in detail the processes occurring there under well-controlled experimental conditions. Planar lipid bilayers make it possible to record single conduction events with a measured current of the order of a tenth of a picoampere. The relative simplicity of the method, the possibility of observing single molecular events and the high reproducibility of the results determine the unprecedented effectiveness of using planar lipid bilayers to identify key physical and chemical factors responsible for the regulation of the functioning of ion channels. This review is a collection of published data on the mechanisms of regulation of ion channels associated with the lipid microenvironment formed by various antimicrobial agents. The analysis allows us to consider lipids as molecular chaperones that ensure the formation of pores in targeted membranes by antimicrobial agents.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"257 - 273"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid-Mediated Adaptation of Proteins and Peptides in Cell Membranes","authors":"A. A. Polyansky,&nbsp;R. G. Efremov","doi":"10.1134/S1990747824700235","DOIUrl":"10.1134/S1990747824700235","url":null,"abstract":"<p>The paper overviews the results of computational studies of the molecular mechanisms underlying the adaptation of model cell membranes taking place during their interaction with proteins and peptides. We discuss changes in the structural and dynamic parameters of the water–lipid environment, the hydrophobic/hydrophilic organization of the lipid bilayer surface (the so-called “mosaicity”), etc. Taken together, these effects are called the “membrane response” (MR) and constitute the most important ability of the cell membranes to respond specifically and consistently to the incorporation of extraneous agents, primarily proteins and peptides, and their subsequent functioning. The results of the authors' long-term research in the field of molecular modeling of MR processes with various spatial and temporal characteristics are described, from the effects of binding of individual lipid molecules to proteins to changes in the integral macroscopic parameters of membranes. The bulk of the results were obtained using the “dynamic molecular portrait” approach developed by the authors. The biological role of the observed phenomena and potential ways of rationally designing artificial membrane systems with specified MR characteristics are discussed. This, in turn, is important for targeted changes in the activity profile of proteins and peptides exerting action on biomembranes, not least as promising pharmacological agents.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"241 - 256"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To the 90th Anniversary of the Birth of Academician Yuri Anatolievich Ovchinnikov","authors":"","doi":"10.1134/S1990747824700260","DOIUrl":"10.1134/S1990747824700260","url":null,"abstract":"","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"137 - 139"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of 20-Hydroxyecdysone on the Functioning of Isolated Mouse Skeletal Muscle Mitochondria","authors":"A. A. Semenova,&nbsp;A. D. Igoshkina,&nbsp;N. V. Mikina,&nbsp;R. G. Savchenko,&nbsp;L. V. Parfenova,&nbsp;M. V. Dubinin","doi":"10.1134/S1990747824700144","DOIUrl":"10.1134/S1990747824700144","url":null,"abstract":"<p>In this work we have studied the effect of the phytoecdysteroid 20-hydroxyecdysone (20E) on the functioning of mouse skeletal muscle mitochondria. It is shown that 20E at a concentration of 100 µM or more suppresses mitochondrial respiration fueled by glutamate and malate (substrates of complex I of the respiratory chain) or succinate (substrate of complex II of the respiratory chain). This effect of 20E is accompanied by a decrease in the mitochondrial membrane potential and is associated with inhibition of the activity of complex III, the total activity of complexes I + III and II + III of the mitochondrial respiratory chain. We have noted a prooxidant effect of 20E, which manifests itself in an increase in the production of hydrogen peroxide by skeletal muscle mitochondria. In addition, 20E reduces the ability of mitochondria to accumulate calcium ions in the matrix. We discuss the mechanisms of the possible toxic effect of 20E on the functioning of skeletal muscle mitochondria.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 2","pages":"127 - 135"},"PeriodicalIF":1.1,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141519597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Features of Thrombin Binding to Platelet Membrane","authors":"R. R. Kerimov,&nbsp;D. Yu. Nechipurenko,&nbsp;M. A. Panteleev","doi":"10.1134/S1990747824700090","DOIUrl":"10.1134/S1990747824700090","url":null,"abstract":"<p>Thrombin is a key enzyme of the blood coagulation system, which has been actively studied since the beginning of the last century. The formation of thrombin from prothrombin in the vicinity of vessel injury leads not only to the formation of fibrin—an important structural component of the hemostatic clot—but also to the activation of platelets, endothelium and immune system cells. The binding of thrombin to the platelet surface is thought to play a critical role in the process of platelet activation and may also ensure the maintenance of a high concentration of thrombin within the thrombus due to the concentration of protease on the platelet surface. Nowadays, all major thrombin receptors on platelets have been thoroughly characterized: through various experimental methods, the physicochemical parameters of the corresponding intermolecular interactions have been established. Since the interaction of thrombin with platelets leads to their activation, which includes changes in the number of receptors as a result of granule secretion, the interpretation of the observed kinetic binding curves faces a number of difficulties. It is known that as a result of platelet activation some receptors are able to redistribute on the membrane and form dimers and clusters, which makes the kinetics of thrombin binding to platelets an extremely complex process dependent on many factors, such as activator concentrations, platelet state, and other local parameters of the system. This review aims to describe the current understanding of the interaction of thrombin with the platelet membrane and to outline important unresolved issues in this area of research. The review provides not only information on structural and kinetic features of thrombin binding to individual platelet membrane proteins, but also analyzes the relationship between the relevant interaction parameters and previously obtained data on the integral kinetics of protease binding to the platelet surface.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 2","pages":"70 - 77"},"PeriodicalIF":1.1,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Low-Intensive He-Ne Laser Radiation on the Composition and Content of Phospholipids and Sterols in the Callus Tissues of Wheat Тriticum aestivum L.","authors":"L. V. Dudareva,&nbsp;E. G. Rudikovskaya,&nbsp;N. V. Semenova,&nbsp;A. V. Rudikovskii,&nbsp;V. N. Shmakov","doi":"10.1134/S1990747824700120","DOIUrl":"10.1134/S1990747824700120","url":null,"abstract":"<p>The effect of He-Ne laser radiation on the composition and content of cell membrane components, namely phospholipids (PL) and sterols, in wheat callus tissues was studied by chromato-mass spectrometry and thin-layer chromatography. It was shown that irradiation of calluses with laser light at a dose of 3.6 J/cm² resulted in significant changes in the content of these components. Thus, the content of phosphatidylinositol increased in irradiated callus by 8 times, phosphatidylethonolamine by 2 times, and the content of phosphatidic acid decreased by 20% of the sum of PL. For sterols it was found that irradiation caused the most significant changes in the content of β-sitosterol dominant in plants (increase from 1453 ± 170 μg/g dry weight in unirradiated control to 2001 ± 111 μg/g dry weight 1 h after exposure); due to this, the total content of sterols also increased. The analysis of the obtained results suggests that PLs and sterols, primarily those for which regulatory and signaling functions are known, participate in the reaction of plant tissue to exposure to low-intensity He-Ne laser irradiation. This participation is realized as a stress (nonspecific) response to intense radiation.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 2","pages":"100 - 109"},"PeriodicalIF":1.1,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141519595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrophysiology of the Danio rerio Heart","authors":"A. V. Karpushev,&nbsp;V. B. Mikhailova,&nbsp;A. A. Kostareva,&nbsp;B. S. Zhorov","doi":"10.1134/S1990747824700089","DOIUrl":"10.1134/S1990747824700089","url":null,"abstract":"<p>Tropical teleost fish <i>Danio rerio</i> is increasingly used as a model object for electrophysiological studies of human cardiac physiology and pathology. <i>D. rerio</i> is characterized by the similarity with humans in such functional parameters of the electrical activity of the heart as heart rate, action potential morphology, as well as in a set of ion currents depolarizing and repolarizing the cell membrane. <i>D. rerio</i> is easy to breed, easy to handle experimentally, and easy to genetically modify. This overview presents current data on the structural and functional organization of ion channels in <i>D. rerio</i> heart myocytes.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 2","pages":"55 - 69"},"PeriodicalIF":1.1,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141505519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Feasibility of Using an Acedan-Based Fluorescent Probe to Monitor Hydrogen Sulfide in Primary Neuronal Cultures","authors":"R. R. Sharipov,&nbsp;I. A. Tarzhanov,&nbsp;A. A. Zgodova,&nbsp;Z. V. Bakaeva,&nbsp;A. M. Surin","doi":"10.1134/S1990747824700119","DOIUrl":"10.1134/S1990747824700119","url":null,"abstract":"<p>Hydrogen sulfide (H₂S), which under physiological conditions exists in cells mainly in the form of the HS^– anion, is considered as a gaseous transmitter of inter- and intracellular signals along with nitrogen monoxide and carbon monoxide. Analysis of the dynamics of H₂S content in living cells is impossible without the creation of sensitive and specific probes. The group of K.H. Ahn synthesized several acedan-based compounds, which in the presence of H₂S attached a sulfhydryl group, forming fluorescent carbocyclic compounds. According to the spectral characteristics and reaction rate with H₂S, the optimal substance was P3, which forms the carbocyclic compound csP3 with the same large Stokes shift as P3 (approx. 130 nm) and has a brighter fluorescence. In this work, we tested the suitability of csP3 for recording changes in H₂S in solutions simulating the minimum salt composition of the intracellular medium, as well as in cells of primary neuronal culture from the rat cerebral cortex. It was found that the fluorescence intensity of csP3, which was formed when Na₂S (H₂S donor, 100 and 300 µM) was added to the P3 solution, differed for solutions corresponding in salt composition to the extracellular medium and cytosol. In both cases, fluorescence increased in the presence of bicarbonate (NaHCO₃, 10 mM). A decrease in the polarity of solutions due to the addition of dimethyl sulfoxide (30% by volume) shifted the emission by approx. 10 nm to the shorter wavelength region and doubled the intensity. Glutamate (10 µM, in the presence of 10 µM of glycine, 0 Mg²⁺) increased the fluorescence of the probe, but only in those neurons in which delayed deregulation of calcium homeostasis did not occur. The addition of P3 or csP3 to the cell culture caused a rapid increase in the fluorescent signal, which was replaced by a slow signal growth after 3–5 min. It was concluded that the product of the reaction of P3 with H₂S was sensitive to changes in the salt composition of the intracellular medium and could be redistributed in cells between an aqueous and more hydrophobic environment. These circumstances made it difficult to interpret the growth of P3 fluorescence in cells as a quantitative indicator of the presence of H₂S and required additional studies of the properties of this and structurally related H₂S probes.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 2","pages":"90 - 99"},"PeriodicalIF":1.1,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141519594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}