Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology最新文献_第3页

Multiplexed Detection of Epitope Specific T-Cell Responses Using CD20 Trogocytosis 利用CD20 Trogocytosis多重检测表位特异性t细胞反应

IF 1.4

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-06-13 DOI: 10.1134/S1990747825700151

E. O. Ryzhichenko, A. V. Shabalkina, S. V. Maryanchik, G. V. Sharonov

引用次数: 0

Registration of NADH Photobleaching for Metabolism–Excitation–Contraction Coupling Studies in Layers of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes NADH光漂白在人诱导多能干细胞衍生心肌细胞层代谢-兴奋-收缩耦合研究中的登记

IF 1.4

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-06-13 DOI: 10.1134/S1990747825700187

M. M. Slotvitsky, S. A. Romanova, M. O. Dabizha, R. Alkhateeb, K. I. Agladze, V. A. Tsvelaya

{"title":"Registration of NADH Photobleaching for Metabolism–Excitation–Contraction Coupling Studies in Layers of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes","authors":"M. M. Slotvitsky, S. A. Romanova, M. O. Dabizha, R. Alkhateeb, K. I. Agladze, V. A. Tsvelaya","doi":"10.1134/S1990747825700187","DOIUrl":"10.1134/S1990747825700187","url":null,"abstract":"Investigation of the complex interrelationships of multiple intercellular processes underlying cardiac electrophysiology and pathophysiology requires the dynamic and non-invasive assessment of multiple parameters. Here, we introduce an approach to two-parametric optical mapping, which allows for studying metabolism–excitation–contraction coupling in human heart tissue. We applied this methodology for cardioplegic solution cardiotoxicity testing and to study the modulation of cardiac physiology during hypoxia. Using this approach, we determined the effects of Normacor and Custodiol cardioplegic solutions on human induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs) physiology with possible long-term effects on cardiomyocyte excitability. We revealed a reversible shortening of the action potential during hypoxia with Normacor (326 ± 36 ms in control and 198 ± 41 ms immediately after 4-h hypoxia and hyperkalemia) and observed an irreversible loss of excitability after 4 h of hypoxia under cold ischemia.","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"19 Proceedings","pages":"227 - 233"},"PeriodicalIF":1.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145121837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Quantitative PALM Applicability for Studies of Protein Oligomerization in Different Conditions 探讨定量PALM在不同条件下蛋白质寡聚研究中的适用性

IF 1.4

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-06-13 DOI: 10.1134/S1990747825700060

A. O. Bogorodskiy, D. S. Burkatovsky, T. A. Btaih, V. A. Pyatkina, V. I. Borshchevskiy

引用次数: 0

Modeling of Protein–Protein Interaction within Landau–Ginzburg–Wilson Approach Using α-Synuclein As an Example 以α-Synuclein为例，利用Landau-Ginzburg-Wilson方法建立蛋白质-蛋白质相互作用模型

IF 1.4

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-06-13 DOI: 10.1134/S1990747825700114

A. A. Korneev, S. D. Liubimov, L. D. Zavarzina

引用次数: 0

Investigating Dual Modulatory Role of Mallotoxin on KCNQ1–KCNE Complexes Using Molecular Modeling 利用分子模型研究Mallotoxin对KCNQ1-KCNE复合物的双重调节作用

IF 1.4

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-06-13 DOI: 10.1134/S1990747825700138

L. Mai, V. Novoseletsky

{"title":"Investigating Dual Modulatory Role of Mallotoxin on KCNQ1–KCNE Complexes Using Molecular Modeling","authors":"L. Mai, V. Novoseletsky","doi":"10.1134/S1990747825700138","DOIUrl":"10.1134/S1990747825700138","url":null,"abstract":"The voltage-gated potassium ion channel KCNQ1 has physiological importance in modulation of electrical excitability in cardiac and epithelial cells. Its activity is modulated by certain proteins or small molecules and its dysfunction may result in arrhythmia, increasing risk of sudden death. Recent research has revealed that Mallotoxin (MTX), a bioactive compound derived from the plant Mallotus philippensis enhances the current generated by the KCNQ1–KCNE1 complex while exerting a modest inhibitory effect on the KCNQ1–KCNE3 channel. The molecular mechanisms underlying these contrasting effects remain unclear, given the structural similarities between the KCNE1 and KCNE3 subunits in their transmembrane regions. Therefore, we employed homology modeling to reconstruct a structural model of the KCNQ1–KCNE1 complex based on the cryo-EM structure of the KCNQ1–KCNE3 complex. Computational analyses of the electrostatic potential landscapes revealed significant differences between these complexes, with the KCNQ1–KCNE3 complex exhibiting a unique region of positive electrostatic potential located centrally within the channel, a feature absent in the KCNQ1–KCNE1 complex. Considering that MTX displays negative charge at physiological pH, we propose that MTX preferentially interacts with this positively charged domain in the KCNQ1–KCNE3 channel, leading to ion flow inhibition, which was supported by further molecular docking observation. Moreover, molecular docking studies suggest that MTX augments the activity of the KCNQ1–KCNE1 complex by stabilizing its open-state structure. These findings clarify the dual modulatory role of MTX in the KCNQ1–KCNE complex and advance therapeutic design for ion channelopathies.","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"19 Proceedings","pages":"188 - 193"},"PeriodicalIF":1.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145121833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane Curvature Controls the Efficiency of Oxidative Phosphorylation System 膜曲率控制氧化磷酸化系统的效率

IF 1.4

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-06-13 DOI: 10.1134/S1990747825700059

S. V. Nesterov

引用次数: 0

Expression and Stabilization of Recombinant Human C3aR Receptor 重组人C3aR受体的表达及稳定性研究

IF 1.4

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-06-13 DOI: 10.1134/S1990747825700084

D. A. Dmitrieva, E. A. Smirnova, A. A. Sadova, L. M. Vasilenko, V. I. Borshchevskiy, A. V. Mishin

引用次数: 0

Comparison of Dopamine and Tyramine Action on the Firing Rate of Trigeminal Dural Afferents in Wild Type and DAT-HET Rats 多巴胺和酪胺对野生型和DAT-HET大鼠三叉神经硬脑膜传入事件放电率的影响

IF 1.1

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-04-24 DOI: 10.1134/S1990747824700533

S. Svitko, K. Shaidullova, E. Nevsky, A. Ananev, G. Sitdikova

{"title":"Comparison of Dopamine and Tyramine Action on the Firing Rate of Trigeminal Dural Afferents in Wild Type and DAT-HET Rats","authors":"S. Svitko, K. Shaidullova, E. Nevsky, A. Ananev, G. Sitdikova","doi":"10.1134/S1990747824700533","DOIUrl":"10.1134/S1990747824700533","url":null,"abstract":"Migraine is a widespread disorder which is defined by persistent and recurring headache attacks. Trigeminal-vascular system (TVS), including meningeal afferents of trigeminal ganglion, is considered to be a key structure in generating nociceptive signals in migraine, in which dopaminergic and trace amine-mediated effects remain unexplored. DAT-HET rats, heterozygous of DAT-KO line, provide a useful model for studying the role of moderate changes in DA homeostasis in migraine mechanisms. In this research, we investigated tyramine and dopamine (DA) effects in trigeminal peripheral afferents with elecrophysiological recording of action potentials of the trigeminal nerve in the rat hemiskull preparations. The data obtained indicates that DA application exerts pro-nociceptive action in trigeminal meningeal afferents in both WT (wild type) and DAT-HET groups in similar manner. Tyramine application also exhibited pro-nociceptive action in peripheral afferents, but demonstrated prolonged pro-nociceptive effect in DAT-HET group. Our data contributes to understanding of dopaminergic signaling in TVS by functionally confirming interplay between dopaminergic system and trace amines in peripheral afferents; as well as by demonstrating of novel electrophysiological evidence of tyramine pro-nociceptive action in TVS.","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"19 1","pages":"106 - 114"},"PeriodicalIF":1.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Behavioral Correlates of Migraine, Oxidative Stress, and CGRP Levels in the Experimental Model of Chronic Migraine with Aura in Rats 大鼠先兆性慢性偏头痛实验模型中偏头痛的行为相关性、氧化应激和 CGRP 水平

IF 1.1

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-04-24 DOI: 10.1134/S1990747824700417

K. Bogatova, A. Yakovlev, G. Sitdikova, O. Yakovleva

{"title":"Behavioral Correlates of Migraine, Oxidative Stress, and CGRP Levels in the Experimental Model of Chronic Migraine with Aura in Rats","authors":"K. Bogatova, A. Yakovlev, G. Sitdikova, O. Yakovleva","doi":"10.1134/S1990747824700417","DOIUrl":"10.1134/S1990747824700417","url":null,"abstract":"Migraine is a common chronic neurovascular disease accompanied by unilateral headache, photophobia, allodynia, and autonomic disturbances. About 1/3 of migraine patients have a transient neurological deficit that precedes or overlaps the headache phase, called aura. The main physiological mechanism underlying aura is thought to be cortical spreading depression (CSD), a wave of massive depolarization passing through neurons and glial cells of the cortex. The aim of the present study was to develop the model of chronic migraine with aura in rats and analyze the main behavioral and biochemical correlates of migraine. Male rats were subjected to surgery with the installation of a mini bath providing access to the dura mater above the somatosensory cortex. KCl was applied on the dura mater to induce CSD every other day for 9 days. Sham rats obtained NaCl application according to the same protocol. Behavioral tests were performed before and after application of KCl or NaCl. Repeated KCl application decreased locomotor and exploratory activity, as well as cognitive ability, increased anxiety and induced photophobia and allodynia in rats. Besides, rats with a model of chronic migraine with aura showed increased plasma calcitonin gene-related peptide (CGRP) concentration and decreased glutathione peroxidase activity in brain tissue. In conclusion, the proposed model can be used to study the behavioral correlates of chronic migraine with aura in experimental animals, which will allow us to investigate the underlying mechanisms and develop treatment approaches.","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"19 1","pages":"27 - 34"},"PeriodicalIF":1.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studying Ferritin Self-Assembly Using the Smoluchowski Coagulation Model 用Smoluchowski凝固模型研究铁蛋白自组装

IF 1.1

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-04-24 DOI: 10.1134/S1990747824700442

M. S. Gette, D. M. Fedorov, O. M. Tilinova, Yu. L. Ryzhykau, A. V. Vlasov

{"title":"Studying Ferritin Self-Assembly Using the Smoluchowski Coagulation Model","authors":"M. S. Gette, D. M. Fedorov, O. M. Tilinova, Yu. L. Ryzhykau, A. V. Vlasov","doi":"10.1134/S1990747824700442","DOIUrl":"10.1134/S1990747824700442","url":null,"abstract":"Ferritin is an iron-storage protein complex performing vital functions in various living organisms. Its properties are significantly defined by a topology of the protein globule and the structural arrangement of subunits on its surface. Understanding the mechanisms of ferritin self-assembly could open up new ways of functionalization of its surface coating with a number of applications in biotechnology. In this study, we investigated the self-assembly of ferritin using the Smoluchowski coagulation model. We numerically solved the Smoluchowski differential equations for various models, involving the formation of hybrid ferritins with two types of subunits (for instance, H and L). Our model incorporates different reaction schemes and provides insights into the kinetics of oligomer assembly. The results reveal that our model can accurately describe the temporal dynamics of ferritin assembly, predicting the formation of intermediate states and fully assembled globules. The extension of the model using a four-dimensional coagulation kernel enables a detailed description of hybrid ferritin assembly, offering a new perspective on the complexity of self-assembly processes in heterooligomeric protein systems. Overall, our findings provide a robust framework for understanding the dynamics of ferritin assembly, offering insights that could be generalized to other carriers and help in designing more effective experimental approaches to study these mechanisms.","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"19 1","pages":"46 - 51"},"PeriodicalIF":1.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0