Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology最新文献

{"title":"Interaction of Albumin with Angiotensin-I-Converting Enzyme According to Molecular Modeling Data","authors":"D. A. Belinskaia,&nbsp;N. V. Goncharov","doi":"10.1134/S1990747824700302","DOIUrl":"10.1134/S1990747824700302","url":null,"abstract":"<p>Human serum albumin (HSA) is an endogenous inhibitor of angiotensin-I-converting enzyme (ACE), which is an integral membrane protein catalyzing the cleavage of decapeptide angiotensin I to octapeptide angiotensin II. By inhibiting ACE, HSA plays an important role in the renin-angiotensin-aldosterone system (RAAS). However, little is known about the mechanism of interaction between these proteins, and the structure of the HSA–ACE complex has not yet been experimentally obtained. The aim of the presented work is to investigate the interaction of HSA with ACE by molecular modeling methods. Ten possible HSA–ACE complexes were obtained by macromolecular docking method. The leader complex was selected according to the number of steric and polar contacts between the proteins, and its stability was tested by molecular dynamics (MD) simulation. The possible effect of modifications in the albumin molecule on its interaction with ACE was analyzed. A comparative analysis of the structure of the obtained HSA–ACE complex with the known crystal structure of the HSA complex with neonatal Fc receptor (FcRn) was performed. The obtained results of molecular modeling define a direction for further in vitro studies of the mechanisms of HSA–ACE interaction. Information about these mechanisms will help in the design and improvement of pharmacotherapy aimed at modulating the physiological activity of ACE.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"303 - 312"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulation of the Glycolytic Metabolite Concentration Profile in Mammalian Resting Skeletal Muscles","authors":"M. V. Martinov,&nbsp;F. I. Ataullakhanov,&nbsp;V. M. Vitvitsky","doi":"10.1134/S1990747824700351","DOIUrl":"10.1134/S1990747824700351","url":null,"abstract":"<p>A mathematical model of glycolysis in mammalian skeletal muscles is presented for the first time, in which stationary concentrations of intermediate metabolites of glycolysis are in good agreement with experimental data obtained in resting muscles. The correspondence between model and experimental values of metabolite concentrations was achieved due to the increased inhibitory effect of ATP on pyruvate kinase and a significant decrease in the [NAD⁺]/[NADH] concentration ratio in the cytoplasm of skeletal muscles. At the same time, activation of muscle pyruvate kinase by fructose-1,6-diphosphate was introduced into the model to allow glycolysis to provide the rate of ATP production required during muscle load activation.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"357 - 367"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygenic Photosynthesis: Induction of Chlorophyll a Fluorescence and Regulation of Electron Transport in Thylakoid Membranes In Silico","authors":"A. V. Vershubskii,&nbsp;V. I. Priklonskii,&nbsp;A. N. Tikhonov","doi":"10.1134/S1990747824700326","DOIUrl":"10.1134/S1990747824700326","url":null,"abstract":"<p>The paper describes an extended mathematical model for the regulation of the key stages of electron transfer in the photosynthetic chain of electron transport and the associated processes of trans-thylakoid proton transfer and ATP synthesis in chloroplasts. This model includes primary plastoquinone PQ_A, associated with photosystem 2 (PS2), and secondary plastoquinone PQ_B, exchanging with plastoquinone molecules that are part of the pool of electronic carriers between PS2 and photosystem 1 (PS1). The model adequately describes the multiphase non-monotonic curves of chlorophyll fluorescence induction and the kinetics of P₇₀₀ redox transformations (photoreaction center PS1), plastoquinone, changes in ATP and pH concentrations in lumen (pH_i) and stroma (pH_o) depending on the illuminating conditions of chloroplasts (variation in intensity and spectral composition of light). The results of computer simulation are consistent with experimental data on the kinetics of photoinduced P₇₀₀ transformations in the leaves of higher plants and the induction of chlorophyll <i>a</i> fluorescence. The obtained data are discussed in the context of “short-term” mechanisms of pH-dependent regulation of electron transport in intact chloroplasts (non-photochemical quenching of excitation in PS2 and activation of the Calvin–Benson cycle reactions).</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"324 - 338"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Approach for Analysis of Intracellular Markers in Phosphatidylserine-Positive Platelets","authors":"E. O. Artemenko,&nbsp;S. I. Obydennyi,&nbsp;M. A. Panteleev","doi":"10.1134/S1990747824700296","DOIUrl":"10.1134/S1990747824700296","url":null,"abstract":"<p>Phosphatidylserine- (PS-) positive platelets play an important role in thrombosis and hemostasis. They have high procoagulant activity, the ability to vesiculate, and can aggregate with activated PS-negative platelets. They are found in growing thrombus in vitro, but numerous mysteries remain regarding them. In particular, intracellular signaling and cytoskeletal reorganization in these platelets is poorly studied, since they have the ability to be destroyed by permeabilization, which is necessary for the penetration of antibodies to intracellular markers into the cell. In this work, we propose an approach that allows the analysis of intracellular signaling in calcium ionophore A23187-induced PS-positive platelets using flow cytometry or confocal microscopy. We used the mildest permeabilization of fixed PS-positive platelets using saponin and showed that such permeabilization allows us to significantly preserve PS-positive platelets. As an example, we analyzed the state of the polymerized form of actin in PS-positive platelets and showed that, despite the significant rearrangement of the cytoskeleton that occurs upon activation in such platelets, actin in them is partially presented in a polymerized form.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"296 - 302"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Changes in Fatty Acid Content in Skeletal Muscle of the Long-Tailed Ground Squirrel Urocitellus undulatus","authors":"T. P. Kulagina,&nbsp;I. M. Vikhlyantsev,&nbsp;A. V. Aripovsky,&nbsp;S. S. Popova,&nbsp;A. B. Gapeyev","doi":"10.1134/S199074782470034X","DOIUrl":"10.1134/S199074782470034X","url":null,"abstract":"<p>Seasonal changes in fatty acid composition in four skeletal muscles of the true hibernating Yakutian long-tailed ground squirrel <i>Urocitellus undulatus</i> were studied. Measurements were taken on animals of four experimental groups: summer active, autumn active, winter hibernating, and winter active. An increase in total fatty acids was found in winter in the <i>quadriceps femoris</i> muscle (<i>m. vastus lateralis</i>), <i>triceps</i> forearm muscle (<i>m</i>. <i>triceps</i>), and lumbar muscle (<i>m. psoas</i>). A decrease in the total content of saturated fatty acids in all muscles was observed during the winter period. The increase in the total content of monounsaturated fatty acids in winter hibernating animals occurred in the quadriceps femoris muscle, triceps forearm muscle, and lumbar muscle. In winter active animals, the total content of polyunsaturated fatty acids increased in quadriceps femoris muscle and lumbar muscle. A statistically significant decrease in the content of palmitic acid in hibernating and winter active animals compared to summer and autumn animals was found in all muscles studied. The content of palmitoleic acid increased in hibernating animals in the quadriceps femoris muscle and lumbar muscle. In the triceps forearm muscle, the content of palmitoleic acid was increased in autumn active and winter hibernating animals. The content of oleic acid was elevated in all muscles in winter hibernating animals relative to active autumn animals. Linoleic acid content was significantly increased in winter active animals in all muscles except the calf muscle. Dihomo-gamma-linolenic acid increased in all muscles during the autumn period with a decrease in content in winter hibernating and winter active animals to the level of summer (seasonal) controls. The results obtained indicate that most of the changes in fatty acid composition have the same direction in all four studied skeletal muscles of the long-tailed ground squirrel. The possible role of seasonal changes in fatty acid composition and fatty acid participation in biochemical processes in the muscle tissue of the long-tailed ground squirrel is discussed.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"348 - 356"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Donation As a Mechanism of Participation of Mesenchymal Stromal Cells in Regenerative Processes","authors":"A. D. Krupnova,&nbsp;D. A. Tsomartova,&nbsp;E. V. Chereshneva,&nbsp;M. Yu. Ivanova,&nbsp;E. S. Tsomartova,&nbsp;T. A. Lomanovskaya,&nbsp;M. S. Pavlova,&nbsp;O. V. Payushina","doi":"10.1134/S1990747824700272","DOIUrl":"10.1134/S1990747824700272","url":null,"abstract":"<p>Mesenchymal stromal cells (MSCs) are universal regulators of regenerative processes due to their ability to paracrine release of regulatory molecules or to replace dead cells by differentiation in the appropriate direction. Recently, another mechanism for the beneficial effects of MSCs on damaged tissues has been discovered, namely transfer of mitochondria into its cells in response to stress signals. MSCs can transfer mitochondria through tunnel nanotubes forming a connecting bridge between cells, through gap junctions, by release as part of extracellular vesicles or in the free form, as well as by complete or partial fusion with recipient cells. In the damaged cells that received mitochondria from MSCs, the disturbed energy metabolism is restored and oxidative stress is reduced, which is accompanied by increased survival, and in some cases also by increased proliferation or changes in differentiation status. Restoration of energetics after mitochondria transfer from MSCs has a beneficial effect on functional activity of recipient cells and promotes suppression of inflammatory reactions. It has been repeatedly demonstrated on models of damage of various organs in experimental animals that the transfer of mitochondria from MSCs to target cells makes a significant contribution to the therapeutic efficacy of MSCs. Therefore, methods to enhance mitochondrial donation are currently being searched for. However, it should be taken into account that MSCs are able to transfer mitochondria to malignant cells, thus stimulating tumor growth and increasing its resistance to chemotherapy. These data make us cautious about the prospects of using MSCs in cell therapy, but, on the other hand, they can serve as a basis for searching for new therapeutic targets in the treatment of cancer.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"275 - 284"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cationic and Ionizable Amphiphiles Based on Dihexadecyl Ester of L-Glutamic Acid for Liposomal Transport of RNA","authors":"G. A. Bukharin,&nbsp;U. A. Budanova,&nbsp;Z. G. Denieva,&nbsp;E. V. Dubrovin,&nbsp;Yu. L. Sebyakin","doi":"10.1134/S1990747824700314","DOIUrl":"10.1134/S1990747824700314","url":null,"abstract":"<p>Various RNAs are among the most promising and actively developed therapeutic agents for the treatment of tumors, infectious diseases and a number of other pathologies associated with the dysfunction of specific genes. Some nanocarriers are used for the effective delivery of RNAs to target cells, including liposomes based on cationic and/or ionizable amphiphiles. Cationic amphiphiles contain a protonated amino group and exist as salts in an aqueous environment. Ionizable amphiphiles are a new generation of cationic lipids that exhibit reduced toxicity and immunogenicity and undergo ionization only in the acidic environment of the cell. In this work we developed a scheme for the preparation and carried out the synthesis of new cationic and ionizable amphiphiles based on natural amino acids (<i>L</i>-glutamic acid, glycine, β-alanine, and γ-aminobutyric acid). Cationic and ionizable liposomes were formed based on the obtained compounds, mixed with natural lipids (phosphatidylcholine and cholesterol), and their physicochemical characteristics (particle size, zeta potential, and storage stability) were determined. Average diameter of particles stable for 5–7 days did not exceed 100 nm. Zeta potential of cationic and ionizable liposomes was about 30 and 1 mV, respectively. The liposomal particles were used to form complexes with RNA molecules. Such RNA complexes were characterized by atomic force microscopy and their applicability for nucleic acid transport was determined.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"313 - 323"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure and Functions of the OTOP1 Proton Channel","authors":"K. D. Sladkov,&nbsp;S. S. Kolesnikov","doi":"10.1134/S1990747824700181","DOIUrl":"10.1134/S1990747824700181","url":null,"abstract":"<p>OTOP1 belongs to the otopetrin family of membrane proteins that form proton channels in cells of diverse types. In mammals, OTOP1 is involved in sour transduction in taste cells and contributes to otoconia formation in the inner ear. From the structural point of view, otopetrins, including OTOP1, represent a quasi-tetramer consisting of four α-barrels. The exact transport pathways mediating proton flux through the OTOP1 channel and gating elements modulating its activity are still a matter of debate. This review discusses current data on structural and functional features of OTOP1. Suggested proton transport pathways, regulatory mechanisms, and key amino acid residues determining functionality of the otopetrins are considered. The existing kinetic models of OTOP1 are discussed. Based on revealed functional properties, OTOP1 is suggested to operate as a logical XOR element that allows for proton flux only if transmembrane pH gradient exists.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"175 - 187"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Studies of Ion Channels: Achievements, Problems, and Perspectives","authors":"B. S. Zhorov,&nbsp;D. B. Tikhonov","doi":"10.1134/S199074782470017X","DOIUrl":"10.1134/S199074782470017X","url":null,"abstract":"<p>The superfamily of membrane proteins known as P-loop channels encompasses potassium, sodium, and calcium channels, as well as TRP channels and ionotropic glutamate receptors. An increasing number of crystal and cryo-EM structures are uncovering both general and specific features of these channels. Fundamental folding principles, the arrangement of structural segments, key residues that influence ionic selectivity, gating, and binding sites for toxins and medically relevant ligands have now been firmly established. The advent of AlphaFold2 models represents another significant step in computationally predicting protein structures. Comparison of experimental P-loop channel structures with their corresponding AlphaFold2 models shows consistent folding patterns in experimentally resolved regions. Despite this remarkable progress, many crucial structural details, particularly important for predicting the outcomes of mutations and designing new medically relevant ligands, remain unresolved. Certain methodological challenges currently hinder the direct assessment of such details. Until the next methodological breakthrough occurs, a promising approach to analyzing ion channel structures in greater depth involves integrating various experimental and theoretical methods.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"160 - 174"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Rhodopsin Project","authors":"M. A. Ostrovsky","doi":"10.1134/S1990747824700156","DOIUrl":"10.1134/S1990747824700156","url":null,"abstract":"<p>The review presents the history of the origin, development, and achievements of the Rhodopsin Project organized by Yu.A. Ovchinnikov in 1973. The current state of some issues related to the structure and function of retinal-containing proteins, rhodopsin types I and II, is also considered.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 3","pages":"140 - 148"},"PeriodicalIF":1.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142248553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}