A. O. Bogorodskiy, D. S. Burkatovsky, T. A. Btaih, V. A. Pyatkina, V. I. Borshchevskiy
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引用次数: 0
Abstract
In this work we aimed to investigate in silico the influence of the common experimental factors on the determination of the oligomerization parameter in the quantitative photoactivated localization microscopy (qPALM). qPALM exploits statistical characteristics of the clusters of individual blinks to deduce the oligomerization of the labeled protein. The aim of this work is to investigate how the properties of the label (number of blinks before photobleaching and fraction of incorrectly folded protein), protein surface density and impurities on the surface may influence the determination of the dimerization fraction. To achieve this, we synthesize sets of image series in different conditions, process them using common protocol, and compare the true and obtained dimerization fraction.
期刊介绍：
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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