Jornal Brasileiro de Reproducao Assistida最新文献

{"title":"Safety of Plasma Rich in Growth Factors (PRGF) as additive to healthy human sperm samples: a pilot study.","authors":"Fernando Quintana, Alberto Vendrell, Silvia Perez- Fernandez, Maria de la Fuente, Aitana Merino-Pérez, Marcos Ferrando, Roberto Matorras","doi":"10.5935/1518-0557.20230075","DOIUrl":"10.5935/1518-0557.20230075","url":null,"abstract":"<p><strong>Objective: </strong>The aim of our study was to assess if the addition of PRGF to healthy human sperm affects its motility and vitality.</p><p><strong>Methods: </strong>This was a prospective study, with 44 sperm donors on whom sperm analysis was performed. Nine mL of blood was collected and PRGF was obtained using PRGF-Endoret® technology. The influence of different dilutions of PRGF (5%, 10%, 20%, 40%) applied to 15 sperm donors was compared, and sperm motility was assessed after 30 minutes. In the second part of the study, 29 sperm donors were studied to analyze the influence of 20% dilution of PRGF at 15, 30 and 45 minutes in fresh and thawed sperm samples. Motility was assessed after the addition of PRGF and after analysis each aliquot was frozen. After thawing, concentration and motility were assessed at the same time periods.</p><p><strong>Results: </strong>There were no differences in sperm motility in fresh samples between dilutions of PRGF when assessed 30 minutes after administration, nor between them, nor when compared to the control group immediately prior to treatment. No trend was observed between motility and PRGF dilution in linear regression analysis. There were no significant differences in thawed samples.</p><p><strong>Conclusions: </strong>The administration of 20% PRGF dilution had no effect on sperm motility compared to samples without PRGF. In addition, there was no change in sperm vitality when comparing samples with and without PRGF. More studies focusing on subnormal sperm samples, analyzing different PRGF concentrations and increasing the number of study variables are needed.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"224-233"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of major analgesics on male fertility: A systematic literature review.","authors":"Melissa Figueiredo Capelo, Paula Bruno Monteiro, Beatriz Matos Anastácio","doi":"10.5935/1518-0557.20240020","DOIUrl":"10.5935/1518-0557.20240020","url":null,"abstract":"<p><strong>Objective: </strong>To verify, based on a systematic literature review, the effects of the main analgesics on male fertility.</p><p><strong>Data sources: </strong>The studies were analyzed from the PubMed, SciELO and LILACS databases.</p><p><strong>Study selection: </strong>The articles selected for the present review included: cohort studies; cross-sectional studies, clinical trials; complete studies; studies with animal models that addressed the proposed theme and that were published within the stipulated period from March 1, 2013, to March 31, 2023, in English, Portuguese and Spanish. These would later have to go through inclusion stages such as framing the type of study and exclusion criteria.</p><p><strong>Data collection: </strong>Author's name, year of publication, study population, number of patients, analgesic, administration time, dose, and effect.</p><p><strong>Conclusions: </strong>There are in vitro and in vivo studies that link paracetamol and ibuprofen to endocrine and seminal changes that are harmful to male fertility. However, more clinical research is needed to determine the doses and timing of administration that affect fertility. The effects of aspirin on male fertility are still unclear due to the lack of studies and consistent methodologies. There is not enough research on dipyrone and its relationship with male fertility, requiring more studies in this area.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"331-340"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140307310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unrecognized abdominal pregnancy with six months' evolution revealed by acute intestinal obstruction in women with PCOS.","authors":"Sana Ghades, Abderahmen Daadoucha, Hamed Jemel, Nour Rouis, Mohamed Ridha Fatnassi","doi":"10.5935/1518-0557.20230057","DOIUrl":"10.5935/1518-0557.20230057","url":null,"abstract":"<p><p>Abdominal pregnancy is a rare form of ectopic pregnancy where implantation and development of the egg take place in the peritoneal cavity outside the tubo-uterine mucosa, in contact with intestinal loops. Diagnosis is most often difficult. We report the case of a 32-year-old woman (gravida 1, para 1), with a history of PCOS, diagnosed with abdominal pregnancy at 20 weeks of amenorrhea complicated by acute intestinal obstruction. Diagnosis was confirmed by abdomino-pelvic scan. Surgery was performed with the patient under general anesthesia. She presented a macerated fetus with an infiltration of the placenta causing a perforation of the sigmoid colon and uterus. Hartmann's procedure was performed and the perforation of the uterus was sutured. Abdominal pregnancy remains a rare variety of ectopic pregnancy. Preoperative diagnosis is difficult due to the presence of a variety of non-specific symptoms. This type of ectopic pregnancy remains challenging for gynecologists and radiologists.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"358-361"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Follicular fluid concentration of soluble Human-G Leukocytic Antigen (sHLA-G) in in vitro fertilization cycles of women with and without peritoneal endometriosis.","authors":"Glícia Pinheiro Bezerra, Vanesa K Genro, Carlos Augusto B Souza, João Sabino Cunha-Filho","doi":"10.5935/1518-0557.20240012","DOIUrl":"10.5935/1518-0557.20240012","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this research is to investigate the association between the concentrations of soluble human leukocyte G antigen (sHLA-G) in the follicular fluid (FF) in infertile patients with peritoneal endometriosis submitted to in vitro fertilization.</p><p><strong>Methods: </strong>We performed a cross-sectional study, including ninety-six women undergoing in vitro fertilization (IVF) ageing ≤ 40 years. Infertile patients were classified into two groups: with endometriosis diagnosed by laparoscopy and without endometriosis due to tubal factor. ELISA measured soluble HLA-G in the FF of a pool of punctured (more than 17mm) follicles from women with endometriosis and without endometriosis who were subjected to ovulation induction for IVF. Embryos obtained after fertilization were classified according to the graduated embryo score (GES).</p><p><strong>Results: </strong>Groups were comparables in terms of age, the number of follicles, AMH, FSH and all included reproductive outcomes. There was no association between sHLA-G concentrations and the average score of the generated embryos (p>0.05). Measurement of sHLA-G in the follicle fluid in women with endometriosis and without endometriosis (tubal factor) showed no significant difference (p>0.05). We also compared sHLA-G per follicle and per embryo, which were not different between both groups (p>0.05).</p><p><strong>Conclusions: </strong>Patients with peritoneal endometriosis submitted to IVF did not demonstrate an altered sHLA-G in the follicular fluid compared to the follicular fluid sHLA-G concentration in tubal factor patients. Also, this molecule was not linked to any other reproductive outcome.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"263-268"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detecting partial premature ovulation during follicular aspiration compromises the quantity, but not the quality, of the oocytes retrieved in stimulated in vitro fertilization (IVF) cycles.","authors":"Víctor Hugo Gómez, Cristina Rodríguez-Varela, Elena Labarta, Ernesto Bosch","doi":"10.5935/1518-0557.20240003","DOIUrl":"10.5935/1518-0557.20240003","url":null,"abstract":"<p><strong>Objective: </strong>To analyze if partial premature ovulation (PPO) detection during oocyte pick-up (OPU) impairs the quality of the retrieved oocyte cohort.</p><p><strong>Methods: </strong>The PPO concept refers to the situation when premature ovulation happens only in some of the follicles and it is detected during OPU. This study constitutes a retrospective analysis performed in an infertility clinic (Spain) during 2016-2021 with patients undergoing OPU after controlled ovarian hyperstimulation for an in vitro fertilization (IVF) treatment. Study code: 2110-VLC-091- VG, registered on December 9 2021. Data from women with PPO (n=111) were compared to a matched control sample of cycles without PPO (n=333) at a proportion of 1:3.</p><p><strong>Results: </strong>Cycles were matched for age, body mass index (BMI), treatment year, embryo genetic analysis and stimulation protocol type. The mean numbers of oocytes (6.1 vs. 11.2), mature oocytes (4.7 vs. 8.8), correctly fertilized oocytes (3.6 vs. 6.6) and top-quality blastocysts (0.9 vs. 1.8) were significantly lower in the PPO group than the nonPPO group (p<0.05). However, maturation, fertilization, top-quality blastocyst and pregnancy rates were statistically comparable among groups (p>0.05).</p><p><strong>Conclusions: </strong>Cycles with PPO have fewer available oocytes and, thus, fewer available embryos for transfer, al though their quality is intact, and still offer chances of pregnancy in these cases. Hence cycle cancellation may not be worth associated money, time and morale losses once PPO is detected.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"240-246"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposed classification of adenomyosis in Infertile women to simplify management options undergoing ART.","authors":"Sunita Tandulwadkar, Sneha Mishra, Swapnil Langde, Mily Pandey, Rashmika Gandhi","doi":"10.5935/1518-0557.20240015","DOIUrl":"10.5935/1518-0557.20240015","url":null,"abstract":"<p><strong>Objective: </strong>Adenomyosis associated with subfertility is a situation of a dilemma for the treating clinician as the treatment is highly controversial and there remains an overall lack of consensus regarding the value of conservative surgery with or without medical management to improve reproductive out-comes. Hence we proposed this classification based on mapping of the size of adenomyoma, its location, distance from the endometrial cavity, and any associated endometriosis by studying 100 women with adenomyosis undergoing IVF.</p><p><strong>Methods: </strong>We did a prospective study over 2 years in 100 women with adenomyosis who underwent IVF. They were classified into 4 categories based on our management-based proposed classification and the pregnancy outcomes were studied in each group.</p><p><strong>Results: </strong>According to our classification, 56% of women belonged to grade 1, 24% to grade 2, 8% to grade 3, and 12% to Grade 4 Adenomyosis. The Pregnancy rates were 71% in Grade 1, 66% with Medical management, and 33% with surgical management in Grade 2, Grade 3 were offered surrogacy, and 66% in Grade 4 Adenomyosis.</p><p><strong>Conclusions: </strong>Our classification is simple and allows cost-effective management based on the location and ex-tent of the disease with the help of ultrasonography.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"353-357"},"PeriodicalIF":1.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140294914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrauterine insemination: prognostic factors.","authors":"Carla Maria Franco Dias, Gabriel Borges Tavares Vitorino, Suelen Maria Parizotto Furlan, Rosana Maria Dos Reis, Ana Carolina Japur de Sá Rosa E Silva, Maria Célia Mendes, Rui Alberto Ferriani, Paula Andrea Navarro","doi":"10.5935/1518-0557.20240017","DOIUrl":"10.5935/1518-0557.20240017","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the impact of possible maternal and paternal prognostic factors and ovarian stimulation protocols on clinical pregnancy and live birth rates in intrauterine insemination (IUI) cycles.</p><p><strong>Methods: </strong>Retrospective observational study of 341 IUI cycles performed from January 2016 to November 2020 at the Assisted Reproduction Service of the Clinics Hospital of the Ribeirão Preto Medical School, University of São Paulo. Clinical pregnancy and live birth rates and their potential prognostic factors were evaluated. Wilcoxon's non-parametric test was used to compare quantitative variables, and the chi-square test to compare qualitative variables, adopting a significance level of p<0.05. A logistic regression model was performed to verify which exploratory variables are predictive factors for pregnancy outcome.</p><p><strong>Results: </strong>The ovulation induction protocol using gonadotropins plus letrozole (p=0.0097; OR 4.3286, CI 1.3040 - 14.3684) and post-capacitation progressive sperm ≥ 5million/mL (p=0.0253) showed a statistically significant correlation with the live birth rate. Female and male age, etiology of infertility, obesity, multifollicular growth, endometrial thickness ≥ 7 mm, and time between human chorionic gonadotropin administration and IUI performance were not associated with the primary outcomes. In the group of patients with ideal characteristics (women aged< 40 years, BMI < 30 kg/m2, antral follicle count ≥ 5, partner aged< 45 years, and post-capacitation semen with progressive spermatozoa ≥ 5 million/mL), the rate of clinical pregnancy was 14.8%, while that of live birth, 9.9%.</p><p><strong>Conclusions: </strong>In this study, the ovulation induction protocol with gonadotropins plus letrozole and post-capacitation progressive sperm ≥ 5 million/mL were the only variables that significantly correlated with intrauterine insemination success.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"254-262"},"PeriodicalIF":1.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140307311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poster Presentations - Abstracts of the 3^rd Brazilian Congress of PRONUCLEO and Regional Meeting Brazil Red Latinoamericana de Reproducción Asistida (REDLARA).","authors":"","doi":"10.5935/1518-0557.20240047","DOIUrl":"10.5935/1518-0557.20240047","url":null,"abstract":"","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":"28 28","pages":"370-379"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141076726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spontaneous bilateral tubal ectopic pregnancy: a gynecological challenge.","authors":"Sana Ghades, Abderahmen Daadoucha, Hamed Jemel, Nour Rouis, Mohamed Ridha Fatnassi","doi":"10.5935/1518-0557.20230058","DOIUrl":"10.5935/1518-0557.20230058","url":null,"abstract":"<p><p>Bilateral ectopic pregnancy is very rare. Although the frequency of ectopic bilateral pregnancy has increased with the advent of medically assisted procreation, spontaneous bilateral tubal pregnancies remain rare. Early detection of this type of ectopic pregnancy is important to prevent maternal mortality and morbidity. Conservative surgery must also be considered, as preservation of both tubes is presumed to offer better fertility prospects. We report the case of a 35-year-old patient at five weeks of amenorrhea with bilateral ectopic pregnancy diagnosed based on ultrasound scans and confirmed during laparotomy. A 35-year-old woman with a history of three vaginal deliveries, non-smoker, on contraceptives (microprogestins), presented with pelvic pain and amenorrhea of five weeks. A beta HCG test came back positive. Pelvic ultrasound revealed a moderate hemoperitoneum and an empty uterus with hematometra. It also showed heterogeneous left and right adnexal masses measuring 3 cm and 4 cm, respectively. An emergency laparotomy was performed. Per-operatively, two bilateral tubal pregnancies of 3 cm and 4 cm were founded. The patient received conservative treatment with bilateral salpingotomy. Postoperative management was uneventful. The diagnosis of spontaneous bilateral tubal ectopic pregnancy is rare and often established at the time of surgery, hence the importance of a rigorous and vigilant examination of the two tubes during ultrasound examination and surgery, so as not to miss it and to better prevent maternal mortality. Conservative surgery must be carefully chosen.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"362-364"},"PeriodicalIF":1.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139472500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimüllerian hormone levels and IVF outcomes in polycystic ovary syndrome women: a scoping review.","authors":"Luciana Carvalho Delamuta, Georges Fassolas, João Antonio Dias Júnior, Luiz Fernando de Oliveira Henrique, Felipe Passos Martins Izzo, Carlos Roberto Izzo","doi":"10.5935/1518-0557.20230059","DOIUrl":"10.5935/1518-0557.20230059","url":null,"abstract":"<p><p>Antimüllerian hormone (AMH) is a homodimeric glycoprotein secreted by granulosa cells from primary to large antral follicles, and it plays an important role in the regulation of early follicle growth. It is considered a reliable marker of ovarian reserve and a predictor of ovarian response to controlled stimulation. Polycystic ovary syndrome (PCOS) is an endocrine condition that affects women of reproductive age worldwide, and it is associated with high levels of AMH. PCOS patients may have worse maturation and fertilization rates compared to normo-ovulatory women. Some studies have demonstrated a positive correlation between AMH levels and qualitative aspects of assisted reproduction treatment; but it is not clear whether high levels of both serum and follicular fluid AMH in PCOS patients correlate with in vitro fertilization outcomes. We ran this scoping review of the literature to address this specific question. We comprehensively searched the databases PubMed and Cochrane Library until January 2023. We found that higher AMH levels are associated with higher oocyte yield, but PCOS patients tend to have fewer mature oocytes and impaired embryo quality and implantation rates. Pregnancy rates, however, are not affected by AMH levels or laboratorial outcomes. We also found that higher AMH levels are associated with worse PCOS features.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"299-305"},"PeriodicalIF":1.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11152416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140050591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}