Effects of astaxanthin supplementation during vitrification and liquid nitrogen vapor freezing on motility, morphology, survival, reactive oxygen species (ROS), and DNA fragmentation of post-cryopreserved human sperm.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Sitthanan Thanintranon, Ubol Saeng-Anan, Teraporn Vutyavanich, Waraporn Piromlertamorn, Pareeya Somsak, Usanee Sanmee
{"title":"Effects of astaxanthin supplementation during vitrification and liquid nitrogen vapor freezing on motility, morphology, survival, reactive oxygen species (ROS), and DNA fragmentation of post-cryopreserved human sperm.","authors":"Sitthanan Thanintranon, Ubol Saeng-Anan, Teraporn Vutyavanich, Waraporn Piromlertamorn, Pareeya Somsak, Usanee Sanmee","doi":"10.5935/1518-0557.20240056","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of astaxanthin supplementation in cryopreservation media on post-thawed sperm motility, viability, morphology, reactive oxygen species (ROS), and DNA fragmentation in two cryopreservation techniques using vitrification and liquid nitrogen vapor freezing.</p><p><strong>Methods: </strong>Thirty normozoospermic semen samples were used in the study. Post-prepared semen samples were divided into 1) non-cryopreserved control, 2) and 3) vitrified without (V) and with astaxanthin 0.5 µM (V+ATX), 4) and 5) frozen in liquid nitrogen vapor without (L) and with astaxanthin 0.5 µM (L+ATX).</p><p><strong>Results: </strong>Cryopreservation using vitrification and liquid nitrogen vapor freezing significantly decreased sperm motility and viability and increased ROS levels. However, no changes were seen in sperm morphology or DNA fragmentation. The addition of astaxanthin in cryopreservation media significantly increased post-thawed motility in both vitrification (77.6±8.9% vs. 69.0±9.5% in V+ATX and V) and vapor freezing (57.0±13.3% vs. 47.7±14.6% in L+ATX and L); it significantly increased sperm viability in vitrification (75.0±11.9% vs. 65.9±11.1% in V+ATX and V), and significantly decreased ROS level in both vitrification (4.7 (2.6-8.3) RLU/sec/106 vs. 10.6 (9.4-16.0) RLU/sec/106 in V+ATX and V) and vapor freezing (4.6 (3.3-10.5) RLU/sec/106 vs. 10.3 (7.9-18.6) RLU/ sec/106 in L+ATX and L). Astaxanthin supplementation in cryopreservation media did not affect sperm morphology or DNA fragmentation.</p><p><strong>Conclusions: </strong>Astaxanthin supplementation improved post-cryopreserved sperm motility, decreased ROS levels in both vitrification and liquid nitrogen vapor freezing and improved sperm viability only in the vitrification technique.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jornal Brasileiro de Reproducao Assistida","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5935/1518-0557.20240056","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: To investigate the effect of astaxanthin supplementation in cryopreservation media on post-thawed sperm motility, viability, morphology, reactive oxygen species (ROS), and DNA fragmentation in two cryopreservation techniques using vitrification and liquid nitrogen vapor freezing.

Methods: Thirty normozoospermic semen samples were used in the study. Post-prepared semen samples were divided into 1) non-cryopreserved control, 2) and 3) vitrified without (V) and with astaxanthin 0.5 µM (V+ATX), 4) and 5) frozen in liquid nitrogen vapor without (L) and with astaxanthin 0.5 µM (L+ATX).

Results: Cryopreservation using vitrification and liquid nitrogen vapor freezing significantly decreased sperm motility and viability and increased ROS levels. However, no changes were seen in sperm morphology or DNA fragmentation. The addition of astaxanthin in cryopreservation media significantly increased post-thawed motility in both vitrification (77.6±8.9% vs. 69.0±9.5% in V+ATX and V) and vapor freezing (57.0±13.3% vs. 47.7±14.6% in L+ATX and L); it significantly increased sperm viability in vitrification (75.0±11.9% vs. 65.9±11.1% in V+ATX and V), and significantly decreased ROS level in both vitrification (4.7 (2.6-8.3) RLU/sec/106 vs. 10.6 (9.4-16.0) RLU/sec/106 in V+ATX and V) and vapor freezing (4.6 (3.3-10.5) RLU/sec/106 vs. 10.3 (7.9-18.6) RLU/ sec/106 in L+ATX and L). Astaxanthin supplementation in cryopreservation media did not affect sperm morphology or DNA fragmentation.

Conclusions: Astaxanthin supplementation improved post-cryopreserved sperm motility, decreased ROS levels in both vitrification and liquid nitrogen vapor freezing and improved sperm viability only in the vitrification technique.

在玻璃化和液氮蒸汽冷冻过程中补充虾青素对冷冻保存后人类精子的活力、形态、存活率、活性氧(ROS)和 DNA 断裂的影响。
目的研究在玻璃化和液氮蒸汽冷冻两种冷冻保存技术中,在冷冻保存介质中添加虾青素对解冻后精子活力、存活率、形态、活性氧(ROS)和DNA碎片的影响:研究使用了 30 份正常无精子精液样本。制备后的精液样本分为:1)非冷冻对照组;2)和3)无虾青素(V)和虾青素0.5 µM(V+ATX)的玻璃化;4)和5)无虾青素(L)和虾青素0.5 µM(L+ATX)的液氮蒸汽冷冻:结果:玻璃化冷冻和液氮蒸汽冷冻显著降低了精子的活力和存活率,并增加了ROS水平。但是,精子形态和 DNA 片段没有发生变化。在冷冻培养基中添加虾青素可显著提高玻璃化(77.6±8.9% vs. 69.0±9.5% in V+ATX and V)和汽化冷冻(57.0±13.3% vs. 47.7±14.6% in L+ATX and L)中解冻后的精子活力;可显著提高玻璃化(75.0±11.9% vs. 69.0±9.5% in V+ATX and V)和汽化冷冻(57.0±13.3% vs. 47.7±14.6% in L+ATX and L)中解冻后的精子活力。在玻璃化(4.7 (2.6-8.3) RLU/sec/106 vs. V+ATX 和 V 的 10.6 (9.4-16.0) RLU/sec/106)和汽化冷冻(4.6 (3.3-10.5) RLU/sec/106 vs. L+ATX 和 L 的 10.3 (7.9-18.6) RLU/sec/106)中,它都能显著降低 ROS 水平。)在低温保存介质中补充虾青素不会影响精子形态或DNA碎片:补充虾青素可提高冷冻后精子的活力,降低玻璃化和液氮汽化冷冻中的ROS水平,仅在玻璃化技术中提高精子的存活率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信