Effects of astaxanthin supplementation during vitrification and liquid nitrogen vapor freezing on motility, morphology, survival, reactive oxygen species (ROS), and DNA fragmentation of post-cryopreserved human sperm.
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引用次数: 0
Abstract
Objective: To investigate the effect of astaxanthin supplementation in cryopreservation media on post-thawed sperm motility, viability, morphology, reactive oxygen species (ROS), and DNA fragmentation in two cryopreservation techniques using vitrification and liquid nitrogen vapor freezing.
Methods: Thirty normozoospermic semen samples were used in the study. Post-prepared semen samples were divided into 1) non-cryopreserved control, 2) and 3) vitrified without (V) and with astaxanthin 0.5 µM (V+ATX), 4) and 5) frozen in liquid nitrogen vapor without (L) and with astaxanthin 0.5 µM (L+ATX).
Results: Cryopreservation using vitrification and liquid nitrogen vapor freezing significantly decreased sperm motility and viability and increased ROS levels. However, no changes were seen in sperm morphology or DNA fragmentation. The addition of astaxanthin in cryopreservation media significantly increased post-thawed motility in both vitrification (77.6±8.9% vs. 69.0±9.5% in V+ATX and V) and vapor freezing (57.0±13.3% vs. 47.7±14.6% in L+ATX and L); it significantly increased sperm viability in vitrification (75.0±11.9% vs. 65.9±11.1% in V+ATX and V), and significantly decreased ROS level in both vitrification (4.7 (2.6-8.3) RLU/sec/106 vs. 10.6 (9.4-16.0) RLU/sec/106 in V+ATX and V) and vapor freezing (4.6 (3.3-10.5) RLU/sec/106 vs. 10.3 (7.9-18.6) RLU/ sec/106 in L+ATX and L). Astaxanthin supplementation in cryopreservation media did not affect sperm morphology or DNA fragmentation.
Conclusions: Astaxanthin supplementation improved post-cryopreserved sperm motility, decreased ROS levels in both vitrification and liquid nitrogen vapor freezing and improved sperm viability only in the vitrification technique.
在玻璃化和液氮蒸汽冷冻过程中补充虾青素对冷冻保存后人类精子的活力、形态、存活率、活性氧（ROS）和 DNA 断裂的影响。
目的研究在玻璃化和液氮蒸汽冷冻两种冷冻保存技术中，在冷冻保存介质中添加虾青素对解冻后精子活力、存活率、形态、活性氧（ROS）和DNA碎片的影响：研究使用了 30 份正常无精子精液样本。制备后的精液样本分为：1）非冷冻对照组；2）和3）无虾青素（V）和虾青素0.5 µM（V+ATX）的玻璃化；4）和5）无虾青素（L）和虾青素0.5 µM（L+ATX）的液氮蒸汽冷冻：结果：玻璃化冷冻和液氮蒸汽冷冻显著降低了精子的活力和存活率，并增加了ROS水平。但是，精子形态和 DNA 片段没有发生变化。在冷冻培养基中添加虾青素可显著提高玻璃化（77.6±8.9% vs. 69.0±9.5% in V+ATX and V）和汽化冷冻（57.0±13.3% vs. 47.7±14.6% in L+ATX and L）中解冻后的精子活力；可显著提高玻璃化（75.0±11.9% vs. 69.0±9.5% in V+ATX and V）和汽化冷冻（57.0±13.3% vs. 47.7±14.6% in L+ATX and L）中解冻后的精子活力。在玻璃化（4.7 (2.6-8.3) RLU/sec/106 vs. V+ATX 和 V 的 10.6 (9.4-16.0) RLU/sec/106）和汽化冷冻（4.6 (3.3-10.5) RLU/sec/106 vs. L+ATX 和 L 的 10.3 (7.9-18.6) RLU/sec/106）中，它都能显著降低 ROS 水平。）在低温保存介质中补充虾青素不会影响精子形态或DNA碎片：补充虾青素可提高冷冻后精子的活力，降低玻璃化和液氮汽化冷冻中的ROS水平，仅在玻璃化技术中提高精子的存活率。
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