Jornal Brasileiro de Reproducao Assistida最新文献

{"title":"Healing with Herbs: A Systematic Review of Natural Treatments for Polycystic Ovary Syndrome.","authors":"Rashmi Wani, Mushtaque Shaikh","doi":"10.5935/1518-0557.20240110","DOIUrl":"https://doi.org/10.5935/1518-0557.20240110","url":null,"abstract":"<p><strong>Objective: </strong>With one in ten women globally suffering from it, polycystic ovarian syndrome (PCOS) has recently emerged as one of the most common endocrine multifactorial illnesses. Each patient may not experience all the potential symptoms of PCOS, which include insulin resistance, hirsutism, acanthosis nigricans, hyperandrogenism, weight gain, etc.</p><p><strong>Methods: </strong>Although symptomatic treatments like ovarian drilling procedures or cosmetic lotions to alleviate hirsutism do not address the underlying cause, there is still no comprehensive treatment for PCOS. It is important to take into account the psychological and occupational factors influencing the unfavourable rise in infertility.</p><p><strong>Results: </strong>Changes in lifestyle, nutrition, prolonged diabetes medication, oral contraceptive pills, and ultimately laparoscopic surgery are obstacles to treating the syndrome. To lower the cost, time, and side effects of current treatments, polyhedral formulations must be developed in light of the aforementioned aspects. Since natural resources are being utilized widely, classes of compounds like coumarins, flavones, lignans and terpins have recently drawn the attention of researchers as a potential source of medication for therapy.</p><p><strong>Conclusions: </strong>This article suggests a list of herbal medications that, when combined with other PCOS treatments, can be successful. These herbs may also be sufficient on their own to treat ovarian cysts naturally.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge differences on the impact of age on fertility among fertile and subfertile women.","authors":"Waranya Rugfoong, Jamjit Doungpunta, Natpat Jansaka, Usanee Sanmee","doi":"10.5935/1518-0557.20240112","DOIUrl":"https://doi.org/10.5935/1518-0557.20240112","url":null,"abstract":"<p><strong>Objective: </strong>To explore the knowledge concerning the impact of age on fertility in Thai women among fertile and subfertile women.</p><p><strong>Methods: </strong>A cross-sectional study was conducted at Maharaj Nakorn Chiang Mai Hospital, Thailand. The questionnaires consisted of sociodemographic questions and knowledge pertinent to the impact of age on fertility. Both fertile and subfertile women completed the same questionnaire anonymously.</p><p><strong>Results: </strong>A total of 500 women were included in the study, with two hundred and fifty in each group. The majority of women in both the fertile and subfertile group correctly identified the most fertile age of women (84.8% vs. 84.4%, p=0.902) and the impact of age on infertility treatment (73.2% vs. 84.0%, p=0.003). However, only one-fifth of the participants in both groups correctly identified the age that their fertility starts to decline, show a marked decline and ends. Most women in both groups chose the answer that overestimates their fertility potential. Moreover, 10.8% of subfertile women believed that fertility is not dependent on age and 16.0% believed that age does not affect the success rate of infertility treatment.</p><p><strong>Conclusions: </strong>The overall knowledge of women regarding the impact of age on fertility is relatively poor with no difference between fertile and subfertile women. These findings may indicate an urgent need to increase this area of knowledge in the population.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dydrogesterone is an eligible tool to suppress LH surge in assisted reproduction technologies (ART) cycles.","authors":"Marcelo Marinho de Souza, Maria do Carmo Borges de Souza, Roberto de Azevedo Antunes, Ana Luíza Barbeitas, Verônica de Almeida Raupp, Ana Luísa Bruno Marinho de Souza, Layna Almeida Barbosa da Silva, Ana Cristina Allemand Mancebo, Flávia Fernandes Sequeira","doi":"10.5935/1518-0557.20250003","DOIUrl":"https://doi.org/10.5935/1518-0557.20250003","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate Dydrogesterone's effectiveness in PPOS protocols for IVF/ICSI or oocyte cryopreservation, focusing on LH surge suppression and metaphase II oocyte yield.</p><p><strong>Methods: </strong>A retrospective, comparative, single-center study of 550 IVF/ICSI and 186 oocyte cryopreservation cycles was conducted from January 2018 to December 2020. Exclusion criteria included endometriosis, previous ovarian surgery, ovarian insufficiency, and abnormal FSH/LH levels. Patients received either Follitropin delta (Rekovelle®) or Menotropin (Menopur®). LH surge blockade was achieved with GnRH antagonist (Cetrotide®) or DYG (Duphaston®). Primary outcome was incidence of premature LH surge; secondary outcomes included follicle size on hCG day, metaphase II oocytes, cancelled cycles, and OHSS. ANCOVA analyses were used, with partial squared Eta as the effect size index.</p><p><strong>Results: </strong>Premature LH peak with early follicular rupture occurred in 2 cases in Group 1 (Ant) and 3 cases in Group 2 (DYG), without statistical significance. LH levels on trigger day showed no difference (Ant: 2.63 - SD 1.15 vs. DYG: 2.47 - SD 1.22). Oocyte maturation at metaphase II (MII) stage was similar (6.28 - SD 4.72 vs. 6.71 - SD 4.53). Follicle size differed: fewer follicles ≥ 18 mm in Ant group (3.33 - SD 2.06 vs. 4.19 - SD 2.53; p=0.001), but more 15-17 mm follicles (p=0.024). No moderate to severe OHSS occurred in patients with AMH >3 ng/mL.</p><p><strong>Conclusions: </strong>DYG is an eligible tool for IVF/ICSI cycles intended to freeze-all and oocyte preservation, embryo banking, and preventing OHSS in patients with high AMH levels.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prednisolone at a therapeutic dose is not detrimental to mouse oocyte competence.","authors":"Shruthivishali Muthukumar, Vijeta Shetty, Akshatha Daddangadi, Satish Kumar Adiga, Shubhashree Uppangala","doi":"10.5935/1518-0557.20240108","DOIUrl":"https://doi.org/10.5935/1518-0557.20240108","url":null,"abstract":"<p><strong>Objective: </strong>To examine the impact of PRDL on mouse oocyte developmental competence.</p><p><strong>Methods: </strong>This study was conducted on 6-8-week-old female Swiss albino mice in which different doses of PRDL were administered for three days during superovulation. Oocytes were assessed for quality, and subsequently, embryos derived from these oocytes were evaluated at the blastocyst stage. Simultaneously, oocytes were also exposed to PRDL during in vitro maturation and examined for their spindle and activation potential.</p><p><strong>Results: </strong>Compared to control group, the total cell number of blastocysts in the PRDL group increased. Nevertheless, the incidence of apoptosis was comparable between the groups. However, direct exposure at 10 µM significantly increased spindle abnormalities compared to those in the control group (p<0.05).</p><p><strong>Conclusions: </strong>Short-term PRDL exposure at a therapeutic dose is not detrimental to oocyte developmental potential in vitro. Although the current study confirmed the safety of PRDL in a mouse model, further studies involving long-term exposure are warranted, as patients receive PRDL treatment for extended periods of time.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carrier Frequency of Autosomal Recessive Diseases in a Population Attending a Human Fertility Institute in Colombia.","authors":"Germán David Ospina Idárraga, Iván Darío Montes Suárez, Lina Maria Caicedo Muriel, Katherine Gisell Hernández Osorio, Diana Milena Diaz Corredor, Paola Andrea Montealegre","doi":"10.5935/1518-0557.20240102","DOIUrl":"https://doi.org/10.5935/1518-0557.20240102","url":null,"abstract":"<p><strong>Objective: </strong>To determine the carrier frequency of X-linked and autosomal recessive diseases in patients attending a human fertility institute in Colombia.</p><p><strong>Methods: </strong>This retrospective observational study included patients and gamete donors attending a Human Fertility Institute in Colombia between January 2017 and June 2023. Sociodemographic data and results of Next Generation Sequencing laboratory panels for screening of recessive disease-causing mutations were collected and analyzed.</p><p><strong>Results: </strong>Data from 746 samples were analyzed; 599 (80.3%) were Colombian origin individuals and 147 (19.7%) were foreigners. At least one mutation was detected in 526 (70.5%) individuals. Of note, 893 pathogenic genetic variants were identified.The genetic variants most frequently observed in all the individuals studied were associated with the following diseases (carrier frequency): alpha thalassemia (10.5%), alpha-1 antitrypsin deficiency (10%), congenital adrenal hyperplasia due to 21-hydroxylase deficiency (9.4%), cystic fibrosis (7.3%), spinal muscular atrophy type 1 (5.6%) and Stargardt disease type 1 (5.0%). The most frequent genetic variant observed in the subgroup of Colombian origin individuals was associated with alpha-1 antitrypsin deficiency (11.3%).</p><p><strong>Conclusions: </strong>Information on the frequency of recessive diseases in Colombia is limited. This pioneering carrier genetic screening identified a high percentage of carriers for at least one recessive autosomal or X-linked in the population evaluated. Screening for recessive mutations could lead to an evolution in family planning programs and a decrease in the number of patients affected by recessive disorders. Furthermore, it could become a routine test not only in cases of assisted reproduction but also in cases of natural gestation.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of coital lubricants on sperm motility and vitality.","authors":"Lalita Pradit, Pareeya Somsak, Waraporn Piromlertamorn, Tawiwan Pantasri, Usanee Sanmee","doi":"10.5935/1518-0557.20240113","DOIUrl":"https://doi.org/10.5935/1518-0557.20240113","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of five coital lubricants including KY jelly, Durex, Pre-seed, Vaseline, and Baby oil on sperm motility and vitality.</p><p><strong>Methods: </strong>Raw semen samples from 20 normozoospermic donors were incubated in vaginal fluid simulant (VFS) controls and a 10% concentration of KY jelly, Durex, Pre-seed, Vaseline, and Baby oil. The sperm motility and vitality were evaluated immediately after mixing (0 minutes) and at 30 and 60 minutes.</p><p><strong>Results: </strong>Sperm motility significantly decreased immediately after mixing in all coital lubricants. Pre-seed demonstrated sperm parameters comparable to VFS at any incubation time. KY jelly, Vaseline, and Baby oil reduced the progressive sperm motility at a certain time, both 30 and 60 minutes in KY jelly, at only 30 minutes in Baby oil, and at only 60 minutes in Vaseline. Durex showed a significant decrease in sperm motility at any incubation time and significantly deteriorated sperm vitality at 60 minutes while other lubricants did not affect sperm vitality.</p><p><strong>Conclusions: </strong>Pre-seed was the coital lubricant that had the least negative effect on sperm. It can be an appropriate coital lubricant for the couple who are trying to conceive when lubricant is indicated. In contrast, KY jelly and Durex are the coital lubricants that should be avoided, especially Durex which had the worst effect on both sperm motility and vitality.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth hormone and gonadotropin association: A case report of full-term pregnancy in a patient with panhypopituitarism.","authors":"Letícia Quandt, Markus Berger, Laura Gazal Passos, Juliana Trevisan da Rocha, Isabel Cirne Lima de Oliveira Durli, Ivan Sereno Montenegro, Eduardo Pandolfi Passos, Paula Terraciano","doi":"10.5935/1518-0557.20240115","DOIUrl":"https://doi.org/10.5935/1518-0557.20240115","url":null,"abstract":"<p><p>Hypopituitarism is the inability of the anterior pituitary gland to properly supply the hormone levels. When this disease affects all the hormones produced by the anterior pituitary, it is called panhypopituitarism (PHP). Since pituitary-derived hormones directly influence fertility, often the assisted reproduction techniques are the only option to PHP women have a full-term pregnancy. However, not all patients diagnosed with PHP properly respond to ovulation induction. Thus, a poor response may indicate decreased ovarian reserve or reflect a deficiency in other key components of ovarian function. Here we presented a rare case of a 24-year-old woman diagnosed with PHP and poor response to previous gonadotropin therapy. In our protocol the patient received first growth hormone (GH) replacement for 5 months before starting gonadotropins. When the serum IGF-I (insulin grow factor-I) level normalized, she started ovulation induction with 225 IU/day of human menopausal gonadotropin (hMG). After the ninth day of the cycle, ultrasounds were performed every 2 days to control follicular growth. The puncture of the follicles was performed on the twentieth day of the cycle and a surprising number of 13 oocytes were collected. The oocytes were fertilized by the classical IVF method, resulting in 11 D3 embryos, of which 2 were freshly transferred. Beta hCG hormone levels were determined, and a single fetus pregnancy was confirmed. The birth was by cesarean section at 38 weeks of gestation. Therefore, we conclude that GH/gonadotropin association in ovarian stimulation may improve the follicular recruitment in PHP patients.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic study on candidates for oocyte donation.","authors":"Sara Araújo, Ana Paula Neto, Maria João Pinho, Sofia Dória, Alberto Barros, Filipa Carvalho","doi":"10.5935/1518-0557.20240087","DOIUrl":"10.5935/1518-0557.20240087","url":null,"abstract":"<p><strong>Objective: </strong>There is a rising demand for assisted reproductive medicine, including sperm, oocyte and embryo donation. Besides medical and legal considerations, genetic testing, including carrier screening for multiple autosomal and X-linked recessive disorders plays an essential role in evaluating hereditary risk among donors and therefore exclude them from the donation process.</p><p><strong>Methods: </strong>A retrospective study was conducted on oocyte donors from a private clinic of assisted reproduction who underwent genetic testing between June 2014 and September 2023. Pre and post-test procedures were performed at the private clinic while karyotyping and carrier screening for Cystic Fibrosis, Fragile X syndrome and Spinal Muscular Atrophy were performed at the Genetic Unit of Faculty of Medicine, University of Porto.</p><p><strong>Results: </strong>Among 581 donors, 81 women were excluded from the donation process since 5/563 had an alteration in karyotype, 57/581 were carriers of a Cystic Fibrosis Transmembrane conductance Regulator pathogenic variant or had a 5T allele, 11/394 had Survival of Motor Neuron 1 deletion and 8/426 had an intermediate or premutation allele in Fragile X Messenger Ribonucleoprotein gene. While recommendations from fertility societies advocate for comprehensive screening, opinions differ on the mandatory implementation of expanded carrier screening.</p><p><strong>Conclusions: </strong>In conclusion, the genetic tests and the pre and post-test counseling is imperative to optimize reproductive outcomes in the oocyte donation process.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"61-66"},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assisted Reproductive Technologies in Latin America: the Latin American Registry, 2021.","authors":"Fernando Zegers-Hochschild, Javier A Crosby, Carolina Musri, Fanny Petermann-Rocha, Gustavo Martinez, Hitomi Nakagawa, Carlos Morente, Armando Roque, Ana Palma-Govea","doi":"10.5935/1518-0557.20240107","DOIUrl":"10.5935/1518-0557.20240107","url":null,"abstract":"<p><strong>Research question: </strong>What are the trends and impact of new technologies on the effectiveness and safety of assisted reproductive technology (ART) performed in Latin America during 2021?</p><p><strong>Design: </strong>This was a retrospective collection of cycle-based multinational data obtained from ART procedures performed by 204 accredited institutions in 16 countries.</p><p><strong>Results: </strong>In total 127,351 initiated cycles resulted in 20,032 deliveries and 22,708 births. ART utilization showed great variability, from 623.5 cycles/million inhabitants in Uruguay to fewer than 35 in Guatemala and El Salvador. The proportion of women aged ≥40 years increased to 35.8%, while that of women ≤34 years dropped to 23.9%. Nonetheless, the proportion of single-embryo transfers (SET) increased from 11.9% in the previous decade to 42.4% in 2021. Of 22,708 babies born, 76.8% were singletons, 22.3% twins and 1.0% triplets or more. Intracytoplasmic sperm injection represented 84.5% of fertilization techniques, and blastocyst transfer increased from 49.6% in 2016 to 79.3% in 2021. The delivery rate after fresh blastocyst elective SET was significantly higher than after the transfer of one frozen embryo from a freeze-all cycle (p<0.0001). The number of aspirations leading to preimplantation genetic testing has increased 2.8 times in 5 years and significantly increased delivery rates/transfer at all ages, including in oocyte donation (p≤0.002), and reduced miscarriage in women ≥35 years old. In oocyte donation, delivery rates after the fresh transfer of embryos from vitrified-warmed oocyte cycles generated similar outcomes to frozen embryo transfer. Perinatal mortality increased from 7.7 ‰ in singletons to 21.3 ‰ in twins.</p><p><strong>Conclusions: </strong>The systematic collection of cycle-based multinational data contributes to cooperative sustained development and helps implement evidence-based reproductive decisions.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":"29 1","pages":"167-190"},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic in compared with Zeta potential, MACS and swim up methods, resulted in improved chromatin integrity and high quality sperms.","authors":"Nastran Vahidi, Hossein Eyni, Fatemeh Tanhaye Kalate Sabz, Nima Narimani, Zahra Zandieh, Fatemehsadat Amjadi","doi":"10.5935/1518-0557.20240075","DOIUrl":"10.5935/1518-0557.20240075","url":null,"abstract":"<p><strong>Objective: </strong>Sperm parameters and DNA integrity are crucial factors in ART outcomes. This study compared four sperm preparation methods (microfluidics, MACS, zeta potential, and swim-Up) for sorting spermatozoa with normal parameters and chromatin integrity.</p><p><strong>Methods: </strong>This study evaluated semen samples from 25 couples with male factor infertility. The semen samples were divided into four portions: one prepared by MACS, one by zeta potential, the other by microfluidics, and the last by swim-up. After preparation, sperm viability, motility, and morphology were assessed based on the WHO guidelines. DNA intergrity was assessed by SDF assay, and the CMA3 staining test was used to evaluate sperm chromatin packaging.</p><p><strong>Results: </strong>Compared to other preparation techniques, microfluidic preparation significantly improved sperm parameters, including motility, viability, morphology, and DNA integrity as well as chromatin packaging (p-value <0.05). The results also demonstrated that sperm motility, viability, and sperm DNA integrity as well as chromatin packaging, were not significantly different after preparation with MACS and Zeta potential methods. However, the MACS and Zeta methods produced improved sperm parameters and better DNA integrity than the swim-up method.</p><p><strong>Conclusions: </strong>Our results indicate that microfluidics can improve sperm quality compared to other methods of sperm preparation. When the microfluidic chip is not available, considering the similar results of sperm preparation by MACS and Zeta potential methods, it is preferred to use the Zeta method for the ART cycle due to its simplicity and cost-effectiveness.</p>","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":"35-40"},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11867249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}