Jornal Brasileiro de Reproducao Assistida最新文献

Microfluidic in compared with Zeta potential, MACS and swim up methods, resulted in improved chromatin integrity and high quality sperms. 微流控与 Zeta 电位、MACS 和游动法相比，提高了染色质的完整性和精子的质量。

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-11-14 DOI: 10.5935/1518-0557.20240075

Nastran Vahidi, Hossein Eyni, Fatemeh Tanhaye Kalate Sabz, Nima Narimani, Zahra Zandieh, Fatemehsadat Amjadi

{"title":"Microfluidic in compared with Zeta potential, MACS and swim up methods, resulted in improved chromatin integrity and high quality sperms.","authors":"Nastran Vahidi, Hossein Eyni, Fatemeh Tanhaye Kalate Sabz, Nima Narimani, Zahra Zandieh, Fatemehsadat Amjadi","doi":"10.5935/1518-0557.20240075","DOIUrl":"https://doi.org/10.5935/1518-0557.20240075","url":null,"abstract":"Objective: Sperm parameters and DNA integrity are crucial factors in ART outcomes. This study compared four sperm preparation methods (microfluidics, MACS, zeta potential, and swim-Up) for sorting spermatozoa with normal parameters and chromatin integrity.Methods: This study evaluated semen samples from 25 couples with male factor infertility. The semen samples were divided into four portions: one prepared by MACS, one by zeta potential, the other by microfluidics, and the last by swim-up. After preparation, sperm viability, motility, and morphology were assessed based on the WHO guidelines. DNA intergrity was assessed by SDF assay, and the CMA3 staining test was used to evaluate sperm chromatin packaging.Results: Compared to other preparation techniques, microfluidic preparation significantly improved sperm parameters, including motility, viability, morphology, and DNA integrity as well as chromatin packaging (p-value <0.05). The results also demonstrated that sperm motility, viability, and sperm DNA integrity as well as chromatin packaging, were not significantly different after preparation with MACS and Zeta potential methods. However, the MACS and Zeta methods produced improved sperm parameters and better DNA integrity than the swim-up method.Conclusions: Our results indicate that microfluidics can improve sperm quality compared to other methods of sperm preparation. When the microfluidic chip is not available, considering the similar results of sperm preparation by MACS and Zeta potential methods, it is preferred to use the Zeta method for the ART cycle due to its simplicity and cost-effectiveness.","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality of information on fertility clinic websites accredited by the Latin American Network of Assisted Reproduction. 拉丁美洲辅助生殖网络认可的生育诊所网站的信息质量。

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-11-14 DOI: 10.5935/1518-0557.20240074

Giselle Ferreira de Souza, Eduarda Maia Lima, Ana Clara Muniz Tavares, Camila Alves Rocha, Larissa Cruz de Souza, Júlia Machado Luz Simões, Nicole Oliveira de Araújo, Maria Yzadora Moura Martins, Marcelo Borges Cavalcante

{"title":"Quality of information on fertility clinic websites accredited by the Latin American Network of Assisted Reproduction.","authors":"Giselle Ferreira de Souza, Eduarda Maia Lima, Ana Clara Muniz Tavares, Camila Alves Rocha, Larissa Cruz de Souza, Júlia Machado Luz Simões, Nicole Oliveira de Araújo, Maria Yzadora Moura Martins, Marcelo Borges Cavalcante","doi":"10.5935/1518-0557.20240074","DOIUrl":"https://doi.org/10.5935/1518-0557.20240074","url":null,"abstract":"Objective: To access to reproductive health information on the Internet helps patients understand their infertility journey and make decisions about their treatment. This study aimed to evaluate the quality of fertility clinic websites accredited by the Latin American Network for Assisted Reproduction (REDLARA) using the QUality Evaluation Scoring Tool (QUEST).Methods: This observational, cross-sectional, and online study evaluated the clinic websites registered as accredited centers on the REDLARA website. The QUEST was used for the quality assessment of the websites. Data were collected from the available websites of all accredited fertility clinics between September 2023 and January 2024.Results: A total of 173 websites from fertility clinics accredited by REDLARA were evaluated, and 152 (87.8%) clinics had functioning websites. The majority of analyzed websites were from Brazilian fertility clinics (n=58; 38.1%), followed by Mexican (n=23; 15.1%) and Argentine (n=21; 13.8%). No indication of authorship or username was observed on most websites. Some form of support for the patient-physician relationship was reported by 86.8% of websites. The mean (standard deviation±SD) of the total score obtained by all fertility clinics was 12.73±4.7 (range: 1-26). Brazil had the highest total score (mean±SD=16.03±4.6), whereas Peru had the lowest (6.42±1.7). Statistical analysis revealed a difference in the quality of websites among Latin American countries.Conclusions: The health information disseminated by fertility clinic websites in Latin America is of poor quality. Therefore, REDLARA should implement rules for building good-quality websites.","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of dietary quercetin on retrieved mouse oocytes and in vitro fertilization outcomes. 膳食槲皮素对小鼠卵母细胞提取和体外受精结果的影响

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-11-14 DOI: 10.5935/1518-0557.20240073

Rasrawee Chantrasiri, Pannarai Somboonchai, Waraporn Piromlertamorn, Tawiwan Pantasri, Usanee Sanmee

{"title":"Effects of dietary quercetin on retrieved mouse oocytes and in vitro fertilization outcomes.","authors":"Rasrawee Chantrasiri, Pannarai Somboonchai, Waraporn Piromlertamorn, Tawiwan Pantasri, Usanee Sanmee","doi":"10.5935/1518-0557.20240073","DOIUrl":"https://doi.org/10.5935/1518-0557.20240073","url":null,"abstract":"Objective: To investigate the effects of dietary quercetin on the retrieved mouse oocytes and IVF outcomes.Methods: Female mice were divided into two groups. Mice were given 0.2 mL water without (control group) or with quercetin 30 mg/kg (quercetin group) orally via gavage, once a day for 21 consecutive days. After that female mice were superovulated for in vitro fertilization. We observed the effect of dietary quercetin on the number of retrieved oocytes, oocyte degeneration rate, fertilization rate, blastocyst formation rate, and blastocyst cell numbers.Results: There was no difference in the number of retrieved oocytes per mouse (27.3±6.7 and 27.2±5.8), oocyte fragmentation rate (28.4% and 25.0%), fertilization rate (47.4% and 50.6%) and blastocyst formation rate (34.8% and 34.7%) in the quercetin group compared to the control group. The proportion of hatching and hatched blastocyst was significantly lower in the quercetin group (17.2% and 27.8%, p=0.004) and significantly lower numbers of cells in TE (47.4±15.3 and 57.2±17.7) and total cells (66.2±18.5 and 77.5±20.7) compared to the control group (p=0.001).Conclusions: Dietary quercetin supplementation has a detrimental effect on mouse embryo quality. Moreover, it did not show any beneficial effect on the ovary in both quantity and quality. This finding raises awareness of the general use of dietary quercetin supplements in infertile females.","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142630193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased antinuclear (anti-Sm) antibodies in infertile patients with peritoneal endometriosis. 患有腹膜子宫内膜异位症的不孕患者体内抗核（抗Sm）抗体增加。

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-11-14 DOI: 10.5935/1518-0557.20240070

Renata Fogaça Borges, Vanessa Krebs Genro, Carlos Augusto Bastos de Souza, Tatiana Ferreira Michelon, Isabel Meneghetti Coimbra, João Sabino Lahorgue da Cunha-Filho

引用次数: 0

The effect of single dose of gonadotropin-releasing hormone agonist injection in frozen-thawed embryo transfer on pregnancy outcomes: A systematic review and meta-analysis. 在冷冻解冻胚胎移植中注射单剂量促性腺激素释放激素激动剂对妊娠结局的影响：系统回顾和荟萃分析。

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-10-15 DOI: 10.5935/1518-0557.20240054

Pongpawan Chienvichai, Natpat Jansaka, Usanee Sanmee, Kittipat Charoenkwan

{"title":"The effect of single dose of gonadotropin-releasing hormone agonist injection in frozen-thawed embryo transfer on pregnancy outcomes: A systematic review and meta-analysis.","authors":"Pongpawan Chienvichai, Natpat Jansaka, Usanee Sanmee, Kittipat Charoenkwan","doi":"10.5935/1518-0557.20240054","DOIUrl":"https://doi.org/10.5935/1518-0557.20240054","url":null,"abstract":"This systematic review and meta-analysis of randomized controlled trials aimed to evaluate the effect of a single-dose gonadotropin-releasing hormone agonist administration in the frozen-thawed embryo transfer cycle on pregnancy outcomes. A literature search was strategically conducted using PubMed, EMBASE, and the Cochrane Controlled Trials Register. The primary outcome was the clinical pregnancy rate. The secondary outcomes combined chemical pregnancy rate, implantation rate, ongoing pregnancy rate, live birth rate, miscarriage rate, and extrauterine pregnancy rate. Out of the 1594 citations that were found, only six met the criteria for being included in the meta-analysis. The clinical pregnancy rate was higher in the treatment group than in the control group (52.05% vs. 47.29%; p=0.04; RR=1.09; 95% CI=1.00-1.18). According to subgroup analysis based on the natural cycle, the clinical pregnancy rate with the agonist administration is significantly higher (43.75% vs. 27.35%; p=0.01; RR=1.6; 95% CI=1.10-2.32). However, there was no difference between the groups in terms of artificial cycles (p=0.80; 95% CI=0.96-1.20). The secondary outcomes did not show significant differences. We concluded that supplementing with a single dose of gonadotrophin-releasing hormone agonist can marginally increase the clinical pregnancy rate, particularly in the natural cycle. Other pregnancy outcomes do not improve with the treatment.","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deleterious Effects of Caffeine Consumption on Reproductive Functions of Female Wistar Rats. 摄入咖啡因对雌性 Wistar 大鼠生殖功能的有害影响

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-10-15 DOI: 10.5935/1518-0557.20240055

Eunice Ogunwole, Victor Oghenekparobo Emojevwe, Hannah Bolutife Shittu, Iyanuoluwa Elizabeth Olagoke, Favour Omolewami Ayodele

{"title":"Deleterious Effects of Caffeine Consumption on Reproductive Functions of Female Wistar Rats.","authors":"Eunice Ogunwole, Victor Oghenekparobo Emojevwe, Hannah Bolutife Shittu, Iyanuoluwa Elizabeth Olagoke, Favour Omolewami Ayodele","doi":"10.5935/1518-0557.20240055","DOIUrl":"https://doi.org/10.5935/1518-0557.20240055","url":null,"abstract":"Objective: The deleterious effects of caffeine consumption on reproductive functions of female Wistar rats were investigated in this study.Methods: In this experimental study, 35 female Wistar rats (180-200g) were divided into 7 groups: Control, II-IV received oral caffeine (10, 20, and 40mg/kg/day respectively) for 21 days. V-VII received similar caffeine doses for 21 days, followed by a 21-day withdrawal period. The ovaries, fallopian tubes, and uteri were assessed for levels of malondialdehyde (MDA), nitric oxide (NO), reduced glutathione (GSH), superoxide dismutase (SOD), and catalase activity using spectrophotometry. Serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol levels were measured by ELISA. Organ histology was performed using microscopy. Statistical analysis employed ANOVA with significance at p<0.05.Results: Caffeine caused dose-dependent increases in MDA, NO, and catalase activity in the ovaries, fallopian tubes, and uteri which decreased upon withdrawal. GSH levels in the ovary and fallopian tubes decreased with caffeine intake but recovered during withdrawal. Caffeine reduced estradiol levels in a dose-dependent manner, its withdrawal led to reductions in serum LH at 20 and 40mg/kg/day and FSH at 40mg/kg/day. Histology revealed dose-dependent alterations in ovarian architecture with congested connective tissues. Caffeine caused sloughing of plicae in the muscularis of the fallopian tubes, degenerated epithelial layer in the uterus, and severe inflammation of the myometrial stroma cells that persisted during caffeine withdrawal.Conclusions: Caffeine consumption adversely impacted the female reproductive functions of rats, altering hormonal balance and organ structure which persisted even after caffeine withdrawal.","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of β-caryophyllene on Sperm Cryopreservation. β-茶碱对精子冷冻的影响

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-10-15 DOI: 10.5935/1518-0557.20240060

Emanuela Ribeiro Moura, Marlon de Araújo Castelo Branco, Yndyra Nayan Teixeira Carvalho Castelo Branco, José Adalmir Torres de Souza, Isolda Márcia Rocha do Nascimento, Marcimar Silva Sousa, Nathyelle Maria Sousa de Oliveira, Wanderson Gabriel Gomes de Melo, Dayseanny de Oliveira Bezerra, Marina Rebeca Soares Carneiro de Sousa, Daniela Kunkel, Camila Ernanda Sousa de Carvalho, Maria Christina Sanches Muratori, Antônio de Sousa Júnior, Amilton Paulo Raposo Costa

{"title":"Effect of β-caryophyllene on Sperm Cryopreservation.","authors":"Emanuela Ribeiro Moura, Marlon de Araújo Castelo Branco, Yndyra Nayan Teixeira Carvalho Castelo Branco, José Adalmir Torres de Souza, Isolda Márcia Rocha do Nascimento, Marcimar Silva Sousa, Nathyelle Maria Sousa de Oliveira, Wanderson Gabriel Gomes de Melo, Dayseanny de Oliveira Bezerra, Marina Rebeca Soares Carneiro de Sousa, Daniela Kunkel, Camila Ernanda Sousa de Carvalho, Maria Christina Sanches Muratori, Antônio de Sousa Júnior, Amilton Paulo Raposo Costa","doi":"10.5935/1518-0557.20240060","DOIUrl":"https://doi.org/10.5935/1518-0557.20240060","url":null,"abstract":"Objective: This study aimed to assess the impact of β-caryophyllene (BC) supplementation in the extender on the post-cryopreservation quality of semen from Dorper rams.Methods: Six Dorper rams were utilized for semen collection over 16 weeks, with BC concentrations determined via the MTT test. Animals were divided into a control group and three treatment groups receiving BC at concentrations of 1.0mM, 2.0mM, and 3.0mM in the Trisegg yolk diluent. Semen was cryopreserved and stored in liquid nitrogen for at least 15 days. After thawing, in vitro assessments including CASA, acrosomal integrity, plasma membrane integrity, mitochondrial membrane potential, and thermo-resistance tests were conducted. Additionally, the TBARS assay was performed to evaluate oxidative stress.Results: While BC supplementation did not significantly affect sperm motility, it notably improved mitochondrial potential and mitigated oxidative stress in cryopreserved ram semen.Conclusions: Incorporating β-caryophyllene into the extender exhibited beneficial effects on the quality of Dorper ram semen post-cryopreservation, enhancing mitochondrial functionality and reducing oxidative stress.","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poster Presentation - REDLARA Congress Andean Region 2024 - Santiago - Chile. 海报展示 - 2024 年安第斯地区 REDLARA 大会 - 圣地亚哥 - 智利。

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-10-15 DOI: 10.5935/1518-0557.20240066

引用次数: 0

Effects of astaxanthin supplementation during vitrification and liquid nitrogen vapor freezing on motility, morphology, survival, reactive oxygen species (ROS), and DNA fragmentation of post-cryopreserved human sperm. 在玻璃化和液氮蒸汽冷冻过程中补充虾青素对冷冻保存后人类精子的活力、形态、存活率、活性氧（ROS）和 DNA 断裂的影响。

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-10-01 DOI: 10.5935/1518-0557.20240056

Sitthanan Thanintranon, Ubol Saeng-Anan, Teraporn Vutyavanich, Waraporn Piromlertamorn, Pareeya Somsak, Usanee Sanmee

{"title":"Effects of astaxanthin supplementation during vitrification and liquid nitrogen vapor freezing on motility, morphology, survival, reactive oxygen species (ROS), and DNA fragmentation of post-cryopreserved human sperm.","authors":"Sitthanan Thanintranon, Ubol Saeng-Anan, Teraporn Vutyavanich, Waraporn Piromlertamorn, Pareeya Somsak, Usanee Sanmee","doi":"10.5935/1518-0557.20240056","DOIUrl":"https://doi.org/10.5935/1518-0557.20240056","url":null,"abstract":"Objective: To investigate the effect of astaxanthin supplementation in cryopreservation media on post-thawed sperm motility, viability, morphology, reactive oxygen species (ROS), and DNA fragmentation in two cryopreservation techniques using vitrification and liquid nitrogen vapor freezing.Methods: Thirty normozoospermic semen samples were used in the study. Post-prepared semen samples were divided into 1) non-cryopreserved control, 2) and 3) vitrified without (V) and with astaxanthin 0.5 µM (V+ATX), 4) and 5) frozen in liquid nitrogen vapor without (L) and with astaxanthin 0.5 µM (L+ATX).Results: Cryopreservation using vitrification and liquid nitrogen vapor freezing significantly decreased sperm motility and viability and increased ROS levels. However, no changes were seen in sperm morphology or DNA fragmentation. The addition of astaxanthin in cryopreservation media significantly increased post-thawed motility in both vitrification (77.6±8.9% vs. 69.0±9.5% in V+ATX and V) and vapor freezing (57.0±13.3% vs. 47.7±14.6% in L+ATX and L); it significantly increased sperm viability in vitrification (75.0±11.9% vs. 65.9±11.1% in V+ATX and V), and significantly decreased ROS level in both vitrification (4.7 (2.6-8.3) RLU/sec/106 vs. 10.6 (9.4-16.0) RLU/sec/106 in V+ATX and V) and vapor freezing (4.6 (3.3-10.5) RLU/sec/106 vs. 10.3 (7.9-18.6) RLU/ sec/106 in L+ATX and L). Astaxanthin supplementation in cryopreservation media did not affect sperm morphology or DNA fragmentation.Conclusions: Astaxanthin supplementation improved post-cryopreserved sperm motility, decreased ROS levels in both vitrification and liquid nitrogen vapor freezing and improved sperm viability only in the vitrification technique.","PeriodicalId":46364,"journal":{"name":"Jornal Brasileiro de Reproducao Assistida","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142356142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Presentations - Abstracts of the 28th Annual Congress of the SBRA. Florianópolis/SC - Brazil, 2024. 口头报告 - 第 28 届 SBRA 年会摘要。巴西弗洛里亚诺波利斯，2024 年。

IF 1.8

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-09-23 DOI: 10.5935/1518-0557.20240062

引用次数: 0