β-茶碱对精子冷冻的影响

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Emanuela Ribeiro Moura, Marlon de Araújo Castelo Branco, Yndyra Nayan Teixeira Carvalho Castelo Branco, José Adalmir Torres de Souza, Isolda Márcia Rocha do Nascimento, Marcimar Silva Sousa, Nathyelle Maria Sousa de Oliveira, Wanderson Gabriel Gomes de Melo, Dayseanny de Oliveira Bezerra, Marina Rebeca Soares Carneiro de Sousa, Daniela Kunkel, Camila Ernanda Sousa de Carvalho, Maria Christina Sanches Muratori, Antônio de Sousa Júnior, Amilton Paulo Raposo Costa
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引用次数: 0

摘要

目的本研究旨在评估在扩增剂中添加β-加里叶烯(BC)对多尔巴公羊精液冷冻保存后质量的影响:方法:利用六只多尔巴公羊进行为期 16 周的精液采集,并通过 MTT 试验测定 BC 的浓度。动物被分为对照组和三个处理组,处理组在 Trisegg 卵黄稀释液中分别接受 1.0mM、2.0mM 和 3.0mM 浓度的 BC。精液在液氮中冷冻保存至少 15 天。解冻后,进行体外评估,包括 CASA、顶体完整性、质膜完整性、线粒体膜电位和耐热性测试。此外,还进行了 TBARS 检测以评估氧化应激:结果:虽然补充 BC 对精子活力没有明显影响,但它显著提高了线粒体电位,减轻了低温保存的公羊精液中的氧化应激:结论:在扩增剂中加入 β-加里叶烯对冷冻保存后多尔帕公羊精液的质量有好处,能提高线粒体功能并减少氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of β-caryophyllene on Sperm Cryopreservation.

Objective: This study aimed to assess the impact of β-caryophyllene (BC) supplementation in the extender on the post-cryopreservation quality of semen from Dorper rams.

Methods: Six Dorper rams were utilized for semen collection over 16 weeks, with BC concentrations determined via the MTT test. Animals were divided into a control group and three treatment groups receiving BC at concentrations of 1.0mM, 2.0mM, and 3.0mM in the Trisegg yolk diluent. Semen was cryopreserved and stored in liquid nitrogen for at least 15 days. After thawing, in vitro assessments including CASA, acrosomal integrity, plasma membrane integrity, mitochondrial membrane potential, and thermo-resistance tests were conducted. Additionally, the TBARS assay was performed to evaluate oxidative stress.

Results: While BC supplementation did not significantly affect sperm motility, it notably improved mitochondrial potential and mitigated oxidative stress in cryopreserved ram semen.

Conclusions: Incorporating β-caryophyllene into the extender exhibited beneficial effects on the quality of Dorper ram semen post-cryopreservation, enhancing mitochondrial functionality and reducing oxidative stress.

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来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
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