Deleterious Effects of Caffeine Consumption on Reproductive Functions of Female Wistar Rats.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Eunice Ogunwole, Victor Oghenekparobo Emojevwe, Hannah Bolutife Shittu, Iyanuoluwa Elizabeth Olagoke, Favour Omolewami Ayodele
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引用次数: 0

Abstract

Objective: The deleterious effects of caffeine consumption on reproductive functions of female Wistar rats were investigated in this study.

Methods: In this experimental study, 35 female Wistar rats (180-200g) were divided into 7 groups: Control, II-IV received oral caffeine (10, 20, and 40mg/kg/day respectively) for 21 days. V-VII received similar caffeine doses for 21 days, followed by a 21-day withdrawal period. The ovaries, fallopian tubes, and uteri were assessed for levels of malondialdehyde (MDA), nitric oxide (NO), reduced glutathione (GSH), superoxide dismutase (SOD), and catalase activity using spectrophotometry. Serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol levels were measured by ELISA. Organ histology was performed using microscopy. Statistical analysis employed ANOVA with significance at p<0.05.

Results: Caffeine caused dose-dependent increases in MDA, NO, and catalase activity in the ovaries, fallopian tubes, and uteri which decreased upon withdrawal. GSH levels in the ovary and fallopian tubes decreased with caffeine intake but recovered during withdrawal. Caffeine reduced estradiol levels in a dose-dependent manner, its withdrawal led to reductions in serum LH at 20 and 40mg/kg/day and FSH at 40mg/kg/day. Histology revealed dose-dependent alterations in ovarian architecture with congested connective tissues. Caffeine caused sloughing of plicae in the muscularis of the fallopian tubes, degenerated epithelial layer in the uterus, and severe inflammation of the myometrial stroma cells that persisted during caffeine withdrawal.

Conclusions: Caffeine consumption adversely impacted the female reproductive functions of rats, altering hormonal balance and organ structure which persisted even after caffeine withdrawal.

摄入咖啡因对雌性 Wistar 大鼠生殖功能的有害影响
目的本研究探讨了摄入咖啡因对雌性 Wistar 大鼠生殖功能的有害影响:在这项实验研究中,35 只雌性 Wistar 大鼠(180-200 克)被分为 7 组:对照组、II-IV 组口服咖啡因(分别为 10、20 和 40 毫克/千克/天)21 天。V-VII 组接受类似剂量的咖啡因治疗 21 天,然后是 21 天的停药期。使用分光光度法评估卵巢、输卵管和子宫中丙二醛(MDA)、一氧化氮(NO)、还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和过氧化氢酶活性的水平。血清黄体生成素(LH)、卵泡刺激素(FSH)和雌二醇水平通过酶联免疫吸附法测定。使用显微镜进行器官组织学检查。统计分析采用方差分析,显著性为 p结果:咖啡因会导致卵巢、输卵管和子宫中的 MDA、NO 和过氧化氢酶活性呈剂量依赖性增加,但在停用咖啡因后会降低。摄入咖啡因后,卵巢和输卵管中的 GSH 水平会下降,但在停用咖啡因后会恢复。咖啡因会以剂量依赖的方式降低雌二醇水平,停用咖啡因会导致血清 LH(20 和 40 毫克/千克/天)和 FSH(40 毫克/千克/天)降低。组织学显示,卵巢结构的改变与剂量有关,结缔组织充血。咖啡因导致输卵管肌层浆膜脱落,子宫上皮层退化,子宫肌层基质细胞严重发炎,在停用咖啡因后仍持续存在:结论:摄入咖啡因会对大鼠的雌性生殖功能产生不利影响,改变荷尔蒙平衡和器官结构,即使在停用咖啡因后仍会持续。
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来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
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