Water ResearchPub Date : 2024-12-19DOI: 10.1016/j.watres.2024.123007
Franziska Kirchen, Thomas Fundneider, Randy Schäfer, Ulrich Grabbe, Susanne Lackner
{"title":"Advanced Micropollutant and Phosphorus removal with Superfine Powdered Activated Carbon and Pile Cloth Media Filtration","authors":"Franziska Kirchen, Thomas Fundneider, Randy Schäfer, Ulrich Grabbe, Susanne Lackner","doi":"10.1016/j.watres.2024.123007","DOIUrl":"https://doi.org/10.1016/j.watres.2024.123007","url":null,"abstract":"Organic micropollutants (OMP) are ubiquitous in aquatic ecosystems and have a proven negative impact on the environment and drinking water resources. To remove OMP from municipal wastewater, the use of superfine Powdered Activated Carbon (sPAC) (d<sub>50</sub> = 1.0 µm) compared to Powdered Activated Carbon (PAC) (d<sub>50</sub> = 30.1 µm) was tested in combination with Pile Cloth Media Filtration (PCMF). sPAC was produced by ball milling PAC to a d<sub>50</sub> of 1.0 µm. No difference was found between the grinding time or energy demand when grinding different raw materials. Different Pile Cloth Media (PCM) were investigated for sPAC retention. The Ultrafiber UF-10 demonstrated superior efficiency in removing sPAC and achieved a turbidity reduction of over 90 % and a total phosphorus concentration (TP) of 40 µg/L, outperforming Microfiber PES-14, which only achieved turbidity reductions of 50 – 80 % and TP concentrations of 80 µg/L. The contact time of sPAC and wastewater before the PCMF was between 0.5 and 8 min depending on the filter velocities (v<sub>F</sub>) (1.0 – 9.0 m/h). A contact time < 2 min in front of the PCMF and 6 – 8 mg sPAC/L was necessary for over 80 % removal of OMP across the entire WWTP. Flocculation and coagulation of sPAC with iron(III)chloride was essential for sufficient sPAC removal with PCMF. With dosage of 150 – 500 mg Fe<sup>3+</sup>/g AC and the use of UF-10, a residual concentration of sPAC in the PCMF effluent of < 0.2 mg/L was achieved. The energy requirement of the process, including the grinding process of the sPAC production, was around 27 Wh/m³ (v<sub>F</sub> = 5 m/h, sPAC = 10 mg/L).","PeriodicalId":443,"journal":{"name":"Water Research","volume":"25 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2024-12-19DOI: 10.1016/j.watres.2024.123013
Min Long, Jie Cheng, Chen Zhou, Bruce E. Rittmann
{"title":"Enhanced Long-term Reduction of High-level Au(III) with the Presence of NO3− in a H2-based Membrane Biofilm Reactor","authors":"Min Long, Jie Cheng, Chen Zhou, Bruce E. Rittmann","doi":"10.1016/j.watres.2024.123013","DOIUrl":"https://doi.org/10.1016/j.watres.2024.123013","url":null,"abstract":"Increased mining and ore processing of gold (Au) are leading to waters contaminated with Au(III) ions, and a common co-contaminant is nitrate (NO<sub>3</sub><sup>−</sup>). Here, we demonstrate that a hydrogen (H<sub>2</sub>)-based membrane biofilm reactor (MBfR) enabled synergistic co-reductions of NO<sub>3</sub><sup>−</sup> to N<sub>2</sub> and Au(III) to elemental Au° for over 250 days of continuous operation. Au(III) was reduced to Au<sup>0</sup> nanoparticles (Au<sup>0</sup>NPs) that were retained within the biofilm's extracellular polymeric substances. NO<sub>3</sub><sup>−</sup> and Au(III) were > 95% reduced at steady state for a wide range of influent conditions: NO<sub>3</sub><sup>−</sup>-N at 1 or 4 mM; Au(III) at 100, 200, or 500 mg/L. Metal-tolerant denitrifiers <em>Azonexus, Pannoibacter, Thermomonas</em>, and <em>Cupriavidus</em> were enriched, as were genes encoding metal reductases. The rate of Au(III) reduction was positively correlated with the abundance of NO<sub>3</sub><sup>−</sup> and NO<sub>2</sub><sup>−</sup> reductases, which supports the role of these reductases in Au(III) reduction. Remarkably, the Au(III)-reduction efficiency remained above 90% in the highly acidic condition, despite NO<sub>2</sub><sup>−</sup> accumulation due to incomplete NO<sub>3</sub><sup>−</sup> reduction; thus, the microbial community was resilient against environmental perturbation. By providing a mechanistic basis for Au recovery using the MBfR, this study establishes the MBfR as a promising and sustainable technology for treating wastewaters containing valuable metals, such as gold, in coordination with microbial denitrification.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"64 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2024-12-19DOI: 10.1016/j.watres.2024.123014
Xueqi Chen, Zhiling Li, Zimeng Zhang, Jun Nan, Guanshu Zhao, Shih-Hsin Ho, Bin Liang, Aijie Wang
{"title":"How Pseudomonas conducts reductive dechlorination of 2,4,6-trichlorophenol: Insights into metabolic performance and organohalide respiration process","authors":"Xueqi Chen, Zhiling Li, Zimeng Zhang, Jun Nan, Guanshu Zhao, Shih-Hsin Ho, Bin Liang, Aijie Wang","doi":"10.1016/j.watres.2024.123014","DOIUrl":"https://doi.org/10.1016/j.watres.2024.123014","url":null,"abstract":"Organohalide-respiring bacteria (OHRB) play a key role in facilitating the detoxification of halogenated organics, but their slow growth and harsh growth conditions often limit their application in field remediation. In this study, we investigated the metabolic performance and organohalide respiration process of a non-obligate OHRB, <em>Pseudomonas</em> sp. CP-1, demonstrating favorable anaerobic reductive dechlorination ability of 2,4,6-trichlorophenol to 4-chlorophenol with a removal rate constant (<em>k</em>) of 0.46 d<sup>-1</sup>. Due to its facultative anaerobic nature, strain CP-1 exhibited unique metabolic properties. In aerobic conditions, strain CP-1 preferentially utilized oxygen for rapid proliferation, and anaerobic reductive dechlorination was initiated once the oxygen was depleted. The aerobic proliferation facilitated the subsequent reductive dechlorination process. Through multi-tool analysis, a modified tricarboxylic acid cycle was proposed to be linked to organohalide respiration when acetate served as the sole carbon source. A predictive model for the electron transport chain (ETC) for reductive dechlorination was constructed, with complex Ⅰ, complex Ⅱ, ubiquinone, complex Fix (flavoprotein), and reductive dehalogenase (RDase) as the major components. A specific RDase facilitating reductive dechlorination was identified. It shared a 64.35% amino acid similarity with biochemically characterized RDases and was designated CprA-2. Its <em>ortho</em>-dechlorination catalytic process was proposed through molecular docking. The discovery of highly adaptable <em>Pseudomonas</em> with favorable dechlorination activity and the elucidation of its metabolic properties provide valuable insights into the understanding of non-obligate OHRBs and their application regulation.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"87 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2024-12-18DOI: 10.1016/j.watres.2024.122947
Charuni Wickramarachchi, Robert Niven, Matthias Kramer
{"title":"Numerical plastic transport modelling in fluvial systems: Review and formulation of boundary conditions","authors":"Charuni Wickramarachchi, Robert Niven, Matthias Kramer","doi":"10.1016/j.watres.2024.122947","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122947","url":null,"abstract":"In recent years, it has become clear that plastic pollution poses a significant threat to aquatic environments and human health. Rivers act as entry points for land-based plastic waste, while a certain fraction of entrained plastics is carried into marine environments. As such, the accurate modelling of plastic transport processes in riverine systems plays a crucial role in developing adequate remediation strategies. In this paper, we review the two main multiphase flow numerical approaches used in plastic transport modelling, comprising Lagrangian Transport Models (LTMs) and Eulerian Transport Models (ETMs). Although LTMs and ETMs can be regarded as complementary and equivalent approaches, LTMs focus on the transport trajectories of individual particles, whereas ETMs represent the behaviour of particles in terms of their mass or volume concentrations. Similar results of the two approaches are expected, while our review shows that plastic transport models are yet to be improved, specifically with respect to the formulation and implementation of boundary conditions, comprising plastic interactions with the channel bed, river bank, and the free surface, as well as interactions with biota. We anticipate that an implementation of these boundary conditions will allow better representing different plastic transport modes, including bed load, suspended load, and surface load. Finally, we provide suggestions for future research directions, including a novel threshold formulation for free surface detachment of plastics, and we hope that this review will inspire the plastic research community, thereby triggering new developments in the rapidly advancing field of numerical plastic transport modelling.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"23 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2024-12-18DOI: 10.1016/j.watres.2024.123009
Alan Carlos de Almeida, Rodrigo Moço Batista, Ítalo Braga Castro, Gilberto Fillmann
{"title":"Passive sampling-derived aqueous concentrations of organotins and booster biocides in the largest Port of South America (Southeastern Brazil)","authors":"Alan Carlos de Almeida, Rodrigo Moço Batista, Ítalo Braga Castro, Gilberto Fillmann","doi":"10.1016/j.watres.2024.123009","DOIUrl":"https://doi.org/10.1016/j.watres.2024.123009","url":null,"abstract":"Organotin compounds (OTs) used to be the most widely used biocide in antifouling paint systems, but the International Maritime Organization (IMO) banned them because of their high environmental toxicity to non-target organisms. Currently, at least 25 active ingredients are being employed as biocides in antifouling paint formulations. In the present study, silicone rubber-based passive sampling was used to determine the freely dissolved concentrations (C<sub>w</sub>) of 6 OTs and 4 booster biocides in the water column at the entrance of Santos Port's main navigation channel, the largest Port of South America (southeastern Brazil). Fifteen sampling events of ∼45 days long were conducted over 2 years. C<sub>w</sub> of OTs ranged from 1.1 to 2.5 ng Sn L<sup>−1</sup> for monobutyltin (MBT), 0.2 to 4.7 ng Sn L<sup>−1</sup> for dibutyltin (DBT), and 0.06 to 0.7 ng Sn L<sup>−1</sup> for tributyltin (TBT), while triphenyltin (TPhT), diphenyltin (DPhT), and monophenyltin (MPhT) were always below their limits of detection (<LOD). For booster biocides, C<sub>w</sub> ranged from 3.9 to 6.3 ng L<sup>−1</sup> for diuron, 0.03 to 0.49 ng L<sup>−1</sup> for Irgarol, <0.55 to 44.3 ng L<sup>−1</sup> for DCOIT, and <0.01 to <0.02 ng L<sup>−1</sup> for dichlofluanid. Levels found in the water column are likely explained by a combination of sources, including simultaneous releases from marinas and shipyards located in adjacent areas, dredging operations, intense vessel traffic in the Santos Port, and desorption from APPs previously released along sediments of the Santos-São Vicente Estuarine System (SSES). Although OTs and booster biocide levels in the Port of Santos were relatively lower than in other studies worldwide, levels detected for TBT, DCOIT, and diuron may pose a risk to non-target organisms.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"22 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2024-12-18DOI: 10.1016/j.watres.2024.123008
Yuanyuan Su, Liu Gao, Elvis Genbo Xu, Licheng Peng, Xiaoping Diao, Yumeng Zhang, Ruiqi Bao
{"title":"When Microplastics Meet Microalgae: Unveiling the Dynamic Formation of Aggregates and their Impact on Toxicity and Environmental Health","authors":"Yuanyuan Su, Liu Gao, Elvis Genbo Xu, Licheng Peng, Xiaoping Diao, Yumeng Zhang, Ruiqi Bao","doi":"10.1016/j.watres.2024.123008","DOIUrl":"https://doi.org/10.1016/j.watres.2024.123008","url":null,"abstract":"Microplastics (MPs) commonly coexist with microalgae in aquatic environments, can heteroaggregate during their interaction, and potentially affect the migration and impacts of MPs in aquatic environments. The hetero-aggregation may also influence the fate of other pollutants through MPs' adsorption or alter their aquatic toxicity. Here, we explored the hetero-aggregation process and its key driving mechanism that occurred between green microalga <em>Chlorella vulgaris</em> (with a cell size of 2-10 μm) and two types of MPs (polystyrene and polylactide, 613 μm). Furthermore, we investigated the environmental impacts of the microplastics-microalgae aggregates (MPs-microalgae aggregates) by comparing their adsorption of Cu(II) with that of pristine MPs and evaluating the effects of hetero-aggregation on MPs aging and their toxicity to microalgae. Our results indicated that microalgal colonization occurred on the surface of MPs, possibly through electrostatic interactions, hole-filling, hydrophilic interactions, and algae-bacteria symbiosis. The hetero-aggregation led to a stronger Cu(II) adsorption by MPs-microalgae aggregates than pristine MPs due to electrostatic interactions, coordination, complexation, and ion exchange. Exposure to either MPs (pristine or aged) or Cu(II) inhibited the cell growth of <em>C. vulgaris</em>, while the integrated biomarker response (IBR) showed more pronounced inhibitory effects resulting from aged MPs compared to pristine MPs and an antagonistic effect on microalgae was caused by the co-exposure to MPs and Cu(II). These findings suggest that the hetero-aggregation of MPs and microalgae may alter their environmental fates and co-pollutant toxicity.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"201 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Natural pigments and biogas recovery from cyanobacteria grown in treated wastewater. Fate of organic microcontaminants.","authors":"Marta Bellver, Evelyn Ruales, Rubén Díez-Montero, Mónica Escolà Casas, Víctor Matamoros, Ivet Ferrer","doi":"10.1016/j.watres.2024.123005","DOIUrl":"https://doi.org/10.1016/j.watres.2024.123005","url":null,"abstract":"Cyanobacterial wastewater-based biorefineries are a sustainable alternative to obtain high-value products with reduced costs. This study aimed to obtain phycobiliproteins and carotenoids, along with biogas from a wastewater-borne cyanobacterium grown in secondary effluent from an urban wastewater treatment plant, namely treated wastewater. For the first time, the presence of contaminants of emerging concern in concentrated pigment extracts was assessed. Tertiary wastewater treatment was conducted in a 3 L photobioreactor inoculated with <em>Synechococcus</em> sp., and operated in semi-continuous regime with a hydraulic retention time of 6 days. The carotenoid content was stable (reaching up to 4 mg gDW<sup>-1</sup>) regardless of the wastewater composition, while the phycobiliprotein content (up to 214 mg gDW<sup>-1</sup>) varied according to nitrogen availability. In concentrated pigment extracts, only 3 (out of 20) organic microcontaminants were detected. The biochemical methane potential of pigment-extracted biomass (222 NL CH<sub>4</sub> kg<sup>−1</sup>) was still 72% of raw biomass. In conclusion, a cyanobacteria culture rich in <em>Synechococcus</em> sp. appears as a promising source of bio-based products in a circular economy approach.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"87 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dissolved oxygen depletion in Chinese coastal waters","authors":"Wenxia Zhang, Song Pan, Liuqian Yu, Haiyan Zhang, Fajin Chen, Guisheng Song, Jiatang Hu, Qinsheng Wei, Huade Zhao, Jianfang Chen, Feng Zhou","doi":"10.1016/j.watres.2024.123004","DOIUrl":"https://doi.org/10.1016/j.watres.2024.123004","url":null,"abstract":"Estuarine and coastal environments have experienced dissolved oxygen (DO hereafter) depression and hypoxia due to increasingly intensified anthropogenic eutrophication and climate warming. This review compared diverse systems in Chinese coastal waters that experience DO depletion or hypoxia, aiming to identify essential aspects in advancing the abilities in comprehensively understanding DO dynamics across systems that span wide ranges of physical and biogeochemical environments. The coastal DO depression and relevant ecological consequences around the world are generally overviewed. DO depression in specific systems around Chinese coastal waters, ranging from large estuarine-coastal system to small embayment, are selected to synthetically understand the environment, the controlling processes, the evolution of eutrophication level, and the potential environmental changes under warming trend. Stressed ecosystems would be put at higher risks with high confidence due to increased complexity and uncertainty caused by future socioeconomic transformation and climate warming. This review proposes key aspects to advancing the abilities in predicting, managing, and mitigating DO stress for marine ecosystems in Chinese coastal waters, potentially providing a framework to discuss future DO changes in the coastal waters worldwide.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"50 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2024-12-17DOI: 10.1016/j.watres.2024.122995
Na Zhao, Panqing Qi, Jin Li, Bowei Tan, Weichuan Kong, Hui Lu
{"title":"Tracking the nitrogen transformation in saline wastewater by marine anammox bacteria-based Fe(II)-driven autotrophic denitratation and anammox","authors":"Na Zhao, Panqing Qi, Jin Li, Bowei Tan, Weichuan Kong, Hui Lu","doi":"10.1016/j.watres.2024.122995","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122995","url":null,"abstract":"Marine anammox bacteria-based Fe(II)-driven autotrophic denitratation and anammox (MFeADA) was investigated for nitrogen removal from saline wastewater for the first time. The study demonstrated that varying influent doses of Fe(II), which participate in the Fe cycle, significantly influenced nitrogen removal performance by altering the fate of nitrite. When 50 mg/L Fe(II) was added, the nitrogen removal was mainly performed by the anammox and Fe(II)-driven autotrophic denitratation (FeAD). As the Fe(II) rose to 100-150 mg/L, the anammox, FeAD and Feammox mainly occurred. Optimal nitrogen removal efficiency, reaching 93%, was achieved at an influent Fe(II) concentration of 150 mg/L. As the Fe(II) reached 250 mg/L, however, nitrate was directly reduced to dinitrogen gas by the excessive Fe(II) through the Fe(II)-driven autotrophic denitrification (FeADN). <em>Candidatus</em> Scalindua (4.1%), <em>Marinicella</em> (5.3%) and <em>SM1A02</em> (31.8%) were the dominant functional microbes. In addition, the normalized nitrate reductase abundance was about 3.1 times that of nitrite reductase, leading to the occurrence of FeAD, which achieved a stable nitrite supply for marine anammox bacteria. This novel study can promote the practical implementation of the MFeADA process in nitrogen-laden saline wastewater treatment.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"64 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2024-12-17DOI: 10.1016/j.watres.2024.122964
Qiuyang Tan, Yi Zhu, Yinjun Zhao, Lei Zheng, Xue Wang, Yuzi Xing, Haoming Wu, Qi Tian, Yaoxin Zhang
{"title":"Comparative analysis of niche adaptation strategies of AOA, AOB, and comammox along a gate-controlled river-estuary continuum","authors":"Qiuyang Tan, Yi Zhu, Yinjun Zhao, Lei Zheng, Xue Wang, Yuzi Xing, Haoming Wu, Qi Tian, Yaoxin Zhang","doi":"10.1016/j.watres.2024.122964","DOIUrl":"https://doi.org/10.1016/j.watres.2024.122964","url":null,"abstract":"Ammonia oxidizers are key players in the biogeochemical nitrogen cycle. However, in critical ecological zones such as estuaries, especially those affected by widespread anthropogenic dam control, our understanding of their occurrence, ecological performance, and survival strategies remains elusive. Here, we sampled sediments along the Haihe River-Estuary continuum in China, controlled by the Haihe Tidal Gate, and employed a combination of biochemical and metagenomic approaches to investigate the abundance, activity, and composition of ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), and complete ammonia oxidizers (comammox). We also conducted an extensive comparison of the salinity adaptation mechanisms of different ammonia oxidizers. We found that AOB (57.55 ± 11.46 %) dominated the nitrification process upstream of the tidal gate, while comammox (68.22 ± 14.42 %) played the major role downstream. Redundancy analysis results showed that total nitrogen, ammonium, and salinity were the primary factors influencing the abundance, activity, and contribution of ammonia oxidizers. The abundance and activity of AOB were significantly positively correlated with ammonium. KEGG annotation results showed that AOA <em>Nitrososphaera</em>, AOB <em>Nitrosomonas</em>, and comammox <em>Nitrospira</em> had 7, 31, and 22 genes associated to salinity adaptation, respectively, and were capable of employing both the “salt-in” and “salt-out” strategies. Metagenome assembly results indicated that comammox outperformed AOA primarily in compatible solute accumulation; AOA can synthesize glutamate, whereas comammox <em>Nitrospira</em> can additionally synthesize glycine betaine, choline, and trehalose. The tidal gate caused sharp shifts in ammonium (from 4.10 ± 3.28 mg·kg<sup>−1</sup> to 0.45 ± 0.10 mg·kg<sup>−1</sup>) and salinity (from 1.64 ± 0.48 ppt to 3.26 ± 0.89 ppt), playing a dominant role in driving niche differentiation of ammonia oxidizers along the Haihe River-Estuary continuum. These findings provide profound insights into the nitrogen cycle in freshwater-saltwater transition zones, especially in today's world where estuaries are widely controlled by tidal gates.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"10 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}