Water Research最新文献_第2页

The Gym Effect: Unveiling the Role of Microbial Adaptation in Organic Carbon Redistribution and Metabolic Optimization in Anaerobic Side-Stream Reactors 健身房效应：揭示微生物适应在厌氧侧流反应器中有机碳再分配和代谢优化中的作用

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-30 DOI: 10.1016/j.watres.2025.123933

Huanzhong Deng, Chuanhan Chen, Lianpeng Sun, Hui Lu, Miao Wang, Xinzhe Zhu, Chao Yang, Ruo-hong Li

{"title":"The Gym Effect: Unveiling the Role of Microbial Adaptation in Organic Carbon Redistribution and Metabolic Optimization in Anaerobic Side-Stream Reactors","authors":"Huanzhong Deng, Chuanhan Chen, Lianpeng Sun, Hui Lu, Miao Wang, Xinzhe Zhu, Chao Yang, Ruo-hong Li","doi":"10.1016/j.watres.2025.123933","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123933","url":null,"abstract":"Although anaerobic side-stream reactor (ASSR) technology has been proven effective in achieving sludge reduction, current research primarily focuses on specific cellular processes, such as cell lysis-cryptic growth, uncoupled metabolism, and endogenous metabolism. This narrow perspective overlooks the holistic impact of ASSR on mainstream systems. Therefore, this study developed a novel exogenous organic carbon balance model, providing a quantitative framework for analyzing carbon redistribution within the system. The results showed that alternating aerobic and deep anaerobic conditions induced significant microbial community reorganization, selectively enriching functional microorganisms such as polyphosphate-accumulating organisms and denitrifiers. This adaptation altered organic carbon flow, reducing assimilatory metabolism (from 3606.4 mg COD/d in the control group to 2872.5 and 2184.3 mg COD/d in the ASSR group), while enhancing aerobic dissimilatory metabolism (from 1866.1 mg COD/d in the control group to 2175.8 and 2616.4 mg COD/d in the ASSR group), thereby promoting pollutant mineralization and sludge reduction. The innovative concept of the \"Gym Effect\" was introduced to explain how environmental stress drives microbial adaptation, optimizes metabolic pathways, and enhances pollutant (nitrogen and phosphorus) removal processes (from 34.8 mg COD/g VSS·h in the control group to 49.8 and 66.7 mg COD/g VSS·h in the ASSR group). These findings redefine our understanding of the mechanisms underlying sludge reduction, establishing the relationship between sludge reduction and organic carbon flow redistribution, and providing a robust theoretical foundation for enhancing sludge reduction strategies.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"41 1","pages":"123933"},"PeriodicalIF":12.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ generation of Cu(III) synergized with crystalline amorphous strong interfacial interaction for autocatalytic degradation of LEV 原位生成Cu（III）与结晶非晶强界面相互作用协同自催化降解LEV

IF 11.4 1区环境科学与生态学

Water Research Pub Date : 2025-05-29 DOI: 10.1016/j.watres.2025.123873

Chun Zhang , Qin Chen , Yuxuan Li , Zhiyong Cai , Zhiguo Wang , Wei Huang , Peng Yu

{"title":"In situ generation of Cu(III) synergized with crystalline amorphous strong interfacial interaction for autocatalytic degradation of LEV","authors":"Chun Zhang , Qin Chen , Yuxuan Li , Zhiyong Cai , Zhiguo Wang , Wei Huang , Peng Yu","doi":"10.1016/j.watres.2025.123873","DOIUrl":"10.1016/j.watres.2025.123873","url":null,"abstract":"<div><div>The practical potential of metal catalysts in the advanced oxidation degradation of antibiotics has been seriously limited due to the secondary pollution caused by their application. To tackle this challenge, this study proposes an innovative approach that utilizes attapulgite/biochar composites for adsorbing heavy metals in wastewater and forming biscuit-type composites with crystalline/amorphous structures to effectively degrade antibiotics. The results show that a bimetallic amorphous layer forms on the surface of materials after Cu and Cd adsorption. This distinctive bimetallic amorphous/crystalline structure can generate highly active intermediates (e.g., Cu(III)) through an electron transfer mechanism, which is essential for the subsequent removal of levofloxacin (LEV). By investigating the formation mechanism of the amorphous/crystalline structure, it is revealed that this structure exhibits superior catalytic performance compared to the crystal structure. Moreover, this research offers an in-depth analysis of the interactions among multiple contaminants, elucidating the pivotal roles played by Cu(III) and Cd in this process. This discovery offers a novel perspective on the practical treatment of wastewater, enhancing not only the efficiency of the process but also the reduction of potential secondary contamination. It also provides a promising avenue for the advancement of environmentally conscious wastewater treatment technology.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"284 ","pages":"Article 123873"},"PeriodicalIF":11.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorising Desalination Brine for Green Cement Production: Toward Mitigating Global CO2 Emissions 绿色水泥生产中海水淡化盐水的增值：减少全球二氧化碳排放

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-29 DOI: 10.1016/j.watres.2025.123930

Zhiyuan Zong, Omar Daoud, Nicholas P. Hankins, Qianhong She, Christian D. Peters

{"title":"Valorising Desalination Brine for Green Cement Production: Toward Mitigating Global CO2 Emissions","authors":"Zhiyuan Zong, Omar Daoud, Nicholas P. Hankins, Qianhong She, Christian D. Peters","doi":"10.1016/j.watres.2025.123930","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123930","url":null,"abstract":"Worldwide, 141.5 million cubic meters of brine are produced by desalination plants per day which is usually rejected and discharged into the ocean without further treatment. Even though some research has been done on brine valorisation, the economic and environmental benefits are rarely understood from a global perspective. This work investigates using desalination brine and low-carbon electricity from renewables as the feedstock in a hybrid process integrating membrane concentration, Chlor-Alkali and mineralisation to produce green MgO cement and other valuable by-products. Under the best practical performance of each individual unit operation, our present-day analysis shows that mitigating CO2 by brine utilisation has higher economic competitiveness ($48 to $61.8 per metric tonne of CO2) compared to other CO2 abatement technologies that are currently available. In addition, the capacity of the proposed process, which is attached to the world’s largest seawater desalination plant, can potentially mitigate 5.45 million tons of CO2 per year. Based on this analysis, it is forecast that, when extrapolated globally with required process tuning based on the regional brine variability, the cement production from the brine utilization process has a theoretical maximum capacity to fulfill up to 14-43% of conventional cement production in 2050. To meet the 2050 net-zero emission goal, utilising rejected desalination brine with green electricity could potentially fulfil up to 47.7% of the global carbon capture sequestration task and mitigate 33.3% to 51.3% of CO2 from the cement industry, which represents 2.6% to 4% of global CO2 emissions. This work offers a novel circular economy pathway that transforms desalination brine—a major water industry by-product—into a high-value, low-carbon construction material. The proposed process advances sustainable water management by linking brine treatment with CO₂ mitigation and cement decarbonization.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"14 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concentration of high boiling point organic solvents by osmotically assisted reverse osmosis 用渗透辅助反渗透法浓缩高沸点有机溶剂

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-29 DOI: 10.1016/j.watres.2025.123929

Mengyang Hu, Kazuo Kumagai, Yasushi Mino, Kecheng Guan, Toshiyuki Kawashima, Hideto Matsuyama

{"title":"Concentration of high boiling point organic solvents by osmotically assisted reverse osmosis","authors":"Mengyang Hu, Kazuo Kumagai, Yasushi Mino, Kecheng Guan, Toshiyuki Kawashima, Hideto Matsuyama","doi":"10.1016/j.watres.2025.123929","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123929","url":null,"abstract":"High boiling point organic solvents (HBOS) are integral to various industrial applications due to their versatile properties. However, the disposal of these solvents raises significant environmental concerns and incurs substantial costs. In this research, an osmotically assisted reverse osmosis (OARO) strategy was utilized to effectively concentrate and treat HBOS (N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAc), and N-methyl-2-pyrrolidone (NMP)) wastewater for recycling of the solvents. The influence of several operational parameters, comprising flow rate, applied pressure, number of membrane modules, and their connection mode, was systematically investigated. Specifically, enhancing the operating pressure and reducing flow rates will both lead to higher concentrations of DMF. Furthermore, we developed a simulation model that can reproduce and predict the OARO process well. Investigating long-term operational stability revealed that the OARO process can sustain stable operations for approximately 18 months. Finally, real DMF wastewater containing 1.0 wt.% DMF from an RO membrane company was treated using the OARO technique, resulting in a successful concentration of approximately 42 times. The findings of this research underscore the significant practical applicability of the OARO strategy for HBOS wastewater recovery, recycling, and reuse.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"16 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144177101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining flow virometry with tree-based machine learning models for rapid virus particle estimation in different wastewater matrices 结合流动病毒学和基于树的机器学习模型快速估计不同废水基质中的病毒颗粒

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-29 DOI: 10.1016/j.watres.2025.123905

Yevhen Myshkevych, Ibrahima N'Doye, Julie Sanchez Medina, Fahad K. Aljehani, Yanghui Xiong, Taous-Meriem Laleg-Kirati, Pei-Ying Hong

{"title":"Combining flow virometry with tree-based machine learning models for rapid virus particle estimation in different wastewater matrices","authors":"Yevhen Myshkevych, Ibrahima N'Doye, Julie Sanchez Medina, Fahad K. Aljehani, Yanghui Xiong, Taous-Meriem Laleg-Kirati, Pei-Ying Hong","doi":"10.1016/j.watres.2025.123905","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123905","url":null,"abstract":"Enumerating virus particles (VPs) at different stages of the wastewater treatment process or along the distribution network is essential for ensuring high performance and reducing public health risks. Herein, we aimed to (i) optimize the flow virometry (FVM) protocol for use in wastewater matrices, (ii) correlate FVM data with specific virus genera of interest, and (iii) develop machine learning (ML) models for determining total VP concentration. We identified and tested a comprehensive set of parameters to determine the optimal conditions for wastewater FVM. Specifically, we tested various sample preprocessing steps to enhance FVM detection sensitivity, including the use of different nucleic acid staining dyes, surfactant addition and concentration optimization, glutaraldehyde fixation, and the effect of sample freezing before FVM analysis. Spearman's rank correlation of FVM data with virus genera concentration using a conventional qPCR-based method in 206 samples showed a positive correlation for all five virus genera, ranging from 0.21 to 0.44 (p < 0.01). The extreme gradient-boosting (XGB) model using easily accessible physiochemical water parameters (such as turbidity, electroconductivity, total dissolved solids, total suspended solids, pH, chemical oxygen demand, and concentrations of nitrate nitrogen, nitrite nitrogen, and ammonium nitrogen) as input data outperformed the random forest (RF) model and can be used to estimate total virus count across all types of wastewater matrices as output data. Furthermore, XGB achieved a better root mean square error in the four treatment processes (influent, aerobic, sand, and MBR) by a mean of 23% than RF in model development. This study demonstrates that FVM, combined with ML, can significantly enhance monitoring capabilities by accurately estimating VP concentrations across diverse wastewater matrices.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"6 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144165136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review in Fe(0)/Fe(Ⅱ) mediated autotrophic denitrification for low C/N wastewater treatment Fe(0)/Fe（Ⅱ）介导的自养反硝化处理低碳氮比废水的研究进展

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-28 DOI: 10.1016/j.watres.2025.123925

Yating Chang, Jizhong Meng, Yuansheng Hu, Po-Heng Lee, Xinmin Zhan

{"title":"A review in Fe(0)/Fe(Ⅱ) mediated autotrophic denitrification for low C/N wastewater treatment","authors":"Yating Chang, Jizhong Meng, Yuansheng Hu, Po-Heng Lee, Xinmin Zhan","doi":"10.1016/j.watres.2025.123925","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123925","url":null,"abstract":"Fe-mediated autotrophic denitrification has emerged as a promising technology for low carbon-to-nitrogen (C/N) wastewater treatment due to its cost-effectiveness, operational safety, and independence from organic carbon. While several reviews have addressed this process, there is still a lack of comprehensive analyses that connect iron materials, denitrification performance, and microbial communities. The practical applicability of solid-phase iron has received little attention, and the key controversial issue - Fe(Ⅱ) oxidation pathway - has not been specifically examined. This review systematically examines both liquid- and solid-phase iron sources, with particular emphasis on the practical applicability of solid-phase iron, and further provides an integrated discussion of denitrification performance and associated functional microbes. In addition, this review summarizes a range of iron-oxidizing denitrifiers and highlights several key genera in detail. It also provides an in-depth analysis of Fe(Ⅱ) oxidation pathways, with particular attention to the ongoing debate regarding the involvement of enzymatic mechanisms. Moreover, the latest advancements in both natural and engineered applications are reviewed. Operational parameters such as temperature (T), pH, dissolved oxygen (DO), Fe/N ratio, and other influencing factors are discussed. Finally, several critical challenges that fundamentally affect Fe-mediated autotrophic denitrification are highlighted. This review aims to support the practical implementation of Fe-mediated autotrophic denitrification in low C/N wastewater treatment and contribute to the sustainable development of environmentally friendly biotechnologies for advanced nitrogen control.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"33 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persulfate activation by biochar for trace organic contaminant removal from urban stormwater 生物炭活化过硫酸盐去除城市雨水中微量有机污染物

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-28 DOI: 10.1016/j.watres.2025.123921

Yiling Zhuang, Stefan B. Haderlein, Holger V. Lutze, Chen Sun, Friedrich Fink, Andrea Paul, Stephanie Spahr

{"title":"Persulfate activation by biochar for trace organic contaminant removal from urban stormwater","authors":"Yiling Zhuang, Stefan B. Haderlein, Holger V. Lutze, Chen Sun, Friedrich Fink, Andrea Paul, Stephanie Spahr","doi":"10.1016/j.watres.2025.123921","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123921","url":null,"abstract":"Persistent and mobile trace organic contaminants (TrOCs) in urban stormwater are difficult to remove through sedimentation- or sorption-based treatment and pose a risk to aquatic ecosystems and drinking water supplies. We demonstrate that the chemical oxidant peroxydisulfate (PDS) can be activated by shrimp shell biochar at pH 7 to form reactive species that selectively react with widespread stormwater contaminants. Of 11 TrOCs tested, oxidative transformation was observed for 1,3-diphenylguanidine, 2-hydroxybenzothiazole, 1H-benzotriazole, 5-methyl-benzotriazole, and diuron during water treatment with biochar and PDS. Laboratory batch experiments conducted with street runoff and a synthetic water showed that the water matrix, containing up to 7.5 mg/L dissolved organic carbon and 100 mM chloride, had a minor effect on the formation of reactive species and contaminant transformation. Using a set of scavengers and probe compounds, we provide evidence for singlet oxygen (1O2) as the predominant reactive species in the biochar/PDS system, which is in agreement with the selectivity of the process to oxidize electron-rich organic contaminants. The results of our study inform new strategies for stormwater treatment using heterogeneous oxidation processes for the abatement of persistent and mobile organic contaminants.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"35 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144165137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decay and Solid-Liquid Partitioning of Mpox and Vaccinia Virus DNA in Primary Influent and Settled Solids to Guide Wastewater-Based Epidemiology Practices m痘和牛痘病毒DNA在初次进水和沉淀固体中的衰变和固液分配以指导基于废水的流行病学实践

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-28 DOI: 10.1016/j.watres.2025.123904

Jacob R. Phaneuf, Gyuhyon Cha, Janet K. Hatt, Konstantinos T. Konstantinidis, Katherine E. Graham

{"title":"Decay and Solid-Liquid Partitioning of Mpox and Vaccinia Virus DNA in Primary Influent and Settled Solids to Guide Wastewater-Based Epidemiology Practices","authors":"Jacob R. Phaneuf, Gyuhyon Cha, Janet K. Hatt, Konstantinos T. Konstantinidis, Katherine E. Graham","doi":"10.1016/j.watres.2025.123904","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123904","url":null,"abstract":"Wastewater-based epidemiology (WBE) has proven to be a powerful tool for tracking the spread of viral pathogens, such as SARS-CoV-2; however, as WBE has expanded to include new pathogens, like mpox virus (MPXV), more data is needed to guide practitioners on how to design WBE campaigns. Here, we investigated the decay rates of heat-inactivated MPXV (HI-MPXV) and attenuated vaccinia virus (VV) viral signal in primary influent and settled solids collected from a local POTW at 4°C, 22°C, or 35°C using digital PCR. Subsequently, we studied the solid-liquid partitioning of the viruses in primary influent. Over 30 days, decay rates did not significantly differ between viruses (p=0.5258). However, decay was significantly higher in primary influent (0.109-0.144/day) than in settled solids (0.019-0.040/day) at both 22°C (p=0.0030) and 35°C (p=0.0166). Furthermore, as part of the partitioning experiment, we found that HI-MPXV and VV adsorb to the solids fraction of primary influent at higher intensities than previously studied enveloped viruses (KF=1,000-31,800 mL/g, n=1.01-1.41). Likewise, it was determined in the partitioning experiment that a concentration of greater than 103 gc/mL in raw influent was needed for the viable quantification of MPXV and VV DNA in the clarified liquid fraction of raw primarily influent. Our study provides essential insights into informative sample collection and storage conditions for the analysis of wastewater and for transport modeling studies. Due to the slow decay observed in settled solids at all tested temperatures in the persistence experiment, this matrix may be most suitable for retrospective analyses of MPXV infections within a community.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"45 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying the underestimated plant potential for phosphorus removal in constructed wetlands: Revealing the mediating mechanism of radial oxygen loss 人工湿地中被低估的植物除磷潜力的量化：揭示径向氧损失的中介机制

IF 11.4 1区环境科学与生态学

Water Research Pub Date : 2025-05-28 DOI: 10.1016/j.watres.2025.123923

Dun Guo , Jingying Zhang , Wei Bian , Yanbin Chi , Bin Li , Qinting Ren , Lei Yang , Jun Lan , Yongxiang Ren

{"title":"Quantifying the underestimated plant potential for phosphorus removal in constructed wetlands: Revealing the mediating mechanism of radial oxygen loss","authors":"Dun Guo , Jingying Zhang , Wei Bian , Yanbin Chi , Bin Li , Qinting Ren , Lei Yang , Jun Lan , Yongxiang Ren","doi":"10.1016/j.watres.2025.123923","DOIUrl":"10.1016/j.watres.2025.123923","url":null,"abstract":"<div><div>Substrate adsorption is considered the primary phosphorus removal pathway in subsurface flow constructed wetlands (CWs), but the role of plants in this process remains underestimated. To clarify this issue, this study conducted root restriction by decreasing substrate depth from 60 cm to 10 and 20 cm to intensify the radial oxygen loss (ROL). On this basis, the resultant enhancement to soluble total phosphorus (TP) removal from late spring to early winter was quantified. Further, the underlying enhancing mechanisms were revealed by analyzing the P mass balance and substrate surface interfacial plant-microbial synergy. CWs shallower than 0.2 m significantly intensified ROL and root biomass, showing 33.4 % more volumetric P removal on average, and the increased rhizosphere P adsorption accounted for all increments. In the rhizosphere, ROL was activated by Fe (II) of Fe3O4 on the substrate surface and produced solid-liquid interfacial-bounded ·OH. Aromatic compounds in root exudations, microbial metabolites, and influent organics that occupied P adsorption sites were mainly converted to aliphatic compounds by ·OH and further biodegraded, thereby re-exposing the adsorption sites. This process contributes to 65.1 % (9.41mgP/M·OH) of the volumetric efficiency increment, and the rest is owing to the ROL-induced P content increasing in extracellular polymeric substances. Larger root biomass created more rhizosphere substrates (accounting for 75.0 %, 56.4 %, and 16.1 % in 0.1, 0.2, and 0.6 m CWs, respectively), amplified the rhizosphere P removal increment, and durably more than halved the effluent TP. Intensifying ROL prolonged the substrate life cycle, and the effective mediation started after 40-day adaptive cultivation in shallow substrates. This study deepened the current theory and inspired CWs’ design and management.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"284 ","pages":"Article 123923"},"PeriodicalIF":11.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Layer-specific photo-metabolic specialization of encapsulated microalgae: A strategy for synchronous multi-nitrogen elimination from wastewater 包封微藻层间光代谢专门化：废水同步多氮消除策略

IF 12.8 1区环境科学与生态学

Water Research Pub Date : 2025-05-28 DOI: 10.1016/j.watres.2025.123926

Meina Han, Shengnan Li, Zhiling Li, Aijie Wang, Nanqi Ren, Shih-Hsin Ho

{"title":"Layer-specific photo-metabolic specialization of encapsulated microalgae: A strategy for synchronous multi-nitrogen elimination from wastewater","authors":"Meina Han, Shengnan Li, Zhiling Li, Aijie Wang, Nanqi Ren, Shih-Hsin Ho","doi":"10.1016/j.watres.2025.123926","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123926","url":null,"abstract":"Conventional wastewater treatment (WWT) systems face persistent challenges in simultaneous ammonium (NH4+-N) and nitrate (NO3--N) removal due to substrate competition and energy-intensive multi-stage processes. This study presents an innovative strategy leveraging encapsulated microalgal systems to achieve synchronous 94.45% NH4+-N and 98.47% NO3--N removal within a single reactor through spatial reprogramming of photosynthetic energy allocation. By exploiting the structural heterogeneity within alginate-encapsulated beads, depth-stratified metabolic zones were created that challenge the long-held dogma of microalgae’s inherent NH4+-N preference. Multidimensional analyses, including spatial distribution mapping, molecular dynamics simulations, metagenomic profiling and photosynthetic regulation, further revealed that light-modulated oxygen gradients, polymer-mediated solute transport, and stratified photo-metabolic specialization synergistically reprogramed microalgal nitrogen metabolism, enabling co-utilization of NH4+-N and NO3--N. The system demonstrated robust dual-nitrogen assimilation efficiencies under varying environmental conditions, transcending conventional substrate utilization hierarchies. This transformative approach not only resolves the dilemma of mixed nitrogen pollution but also advances sustainable WWT by integrating pollutant removal with biomass valorization. The findings provide mechanistic insights into microalgal metabolic plasticity and offer a scalable, energy-efficient solution to upgrade traditional denitrification technologies, aligning with urgent demands for circular economy in water resource management.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"145 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144164793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0