Water Research最新文献

{"title":"Iron clogging mechanism around injection wells during groundwater recharge in shallow iron-rich aquifer: insight from spatiotemporal evolution","authors":"Min Shi, Yuesuo Yang, Dianlong Wang, Yuhui Wu, Xi Zhang, Ying Lu","doi":"10.1016/j.watres.2025.123778","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123778","url":null,"abstract":"Iron deposits in the filters and porous media around injection wells could induce severe clogging, thereby limiting the sustainability of groundwater heat pump (GWHP) systems. During the water injection in shallow iron-rich aquifers, iron clogging exhibits both spatial and temporal evolution in terms of its composition and formation processes. This study employed a two-dimensional sand tank experiment with continuous multicomponent groundwater to explore the spatiotemporal evolution of clogging around the injection well. The results suggested that upper-layer clogging was dominated by high-crystallinity iron oxides, goethite, and lepidocrocite, primarily caused by oxidation corrosion of filter. The evolution of lower-layer clogging occurred in stages with distinct material-mechanism-impact relationships. The initial phase (0–15 days): clogging comprised of calcium carbonate and low-crystallinity iron oxides induced by oxygen enrichment and CO₂ depletion during the early injection period, causing a 10% hydraulic head difference to increase. Mid-stage (15–35 days): microbial aggregates, SiO₂, and lower-crystallinity iron oxides dominated as hydrodynamic shifts elevated pH and redox potential, accelerating microbial growth and iron oxide deposition, amplifying hydraulic head difference to 60%. Stabilization phase (after 35 days): biofilms and extracellular polymeric substances (EPS) secretion by microbial communities consolidated pore occlusion, sustaining stable hydraulic resistance. Each stage transitioned from abiotic mineral precipitation to biologically reinforced clogging, progressively restricting permeability through synergistic physicochemical and microbial processes. This study highlighted a novel understanding of the evolution of iron clogging within an injection well located in a shallow iron-rich aquifer, and proposed region- and stage-specific strategies for clogging mitigation and control.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"110 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoplankton abundance and methane emissions are minimally impacted by environmentally-relevant glyphosate concentrations in small-scale outdoor mesocosms","authors":"Christopher F. Frazier, Ted D. Harris, Tonya DelSontro, Hans-Peter Grossart, Belinda S.M. Sturm, Jalynn M. Murry, Andrew Ising","doi":"10.1016/j.watres.2025.123764","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123764","url":null,"abstract":"Glyphosate is one of the most widely applied agrochemicals in North America and can be directly transported via runoff into non-target aquatic habitats. Yet, our understanding of how this herbicide affects aquatic ecosystems is limited; past studies have often focused on single species effects and/or used herbicide concentrations several orders of magnitude higher than what has been reported in contaminated aquatic systems. Further, glyphosate in aquatic systems has the potential to alter greenhouse gas emissions (methane) if it is broken down for phosphate utilization by bacteria under specific environmental conditions (i.e., oxygen, nutrient concentrations). In this study, we assessed the temporal changes in nutrients, phytoplankton, copy number of genes associated with breakdown of glyphosate or production of methane (<em>phn</em>J, <em>mcr</em>A), and methane concentrations in 12-day mesocosms with amendments of glyphosate, nitrogen, and/or phosphorus. We found glyphosate at environmentally-relevant concentrations (∼4 ug/L) did not confer changes in overall ratios and total concentrations of nutrients, or abundance of any major phytoplankton group (cyanobacteria, diatoms, green algae), methane concentration or flux, or gene copy numbers of <em>phn</em>J and <em>mcr</em>A. Our results suggest that the relatively low concentrations of glyphosate we used (relative to levels used in toxicological studies) did not cause major changes over short time periods in mesocosms, and that the potential for glyphosate to increase greenhouse gas emissions in aquatic systems requires specific conditions to occur and may not be universal in contaminated systems.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"35 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH-Gated Activation of Nematodes-Secreted NUC-1 Accelerates Extracellular Antibiotic Resistance Gene Degradation in Aquatic Environments","authors":"Wenhua Dong, Yi Liu, Manxi Lin, Jianying Zhang, Daohui Lin","doi":"10.1016/j.watres.2025.123788","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123788","url":null,"abstract":"The global dissemination of extracellular antibiotic resistance genes (eARGs) in environmental matrices necessitates urgent development of mitigation approaches. Although nematodes exhibit potential as biological agents for eARG degradation, significant research gaps exist in understanding their performance under diverse environmental conditions and strategies for enhancing degradation efficiency through systematic parameter optimization. Here, we systematically evaluated the degradation of plasmid-borne <em>tetM</em> by <em>Caenorhabditis elegans</em> across eight high nematodes-prevalent habitats, revealing a remarkable 38-fold variation in efficacy. Solution pH was identified as the pivotal regulatory parameter through controlled experiments. Acidification to pH 6 enhanced nematodes-mediated eARG degradation by 25-fold, effectively reducing the transformation efficiency below the detectable limit within 15 min. Through multidisciplinary analyses incorporating gene mutation analysis, mRNA quantification, capillary electrophoresis, and zymographic analysis, we demonstrate that environmental pH specifically modulates NUC-1 activity rather than expression. Structural modeling and p<em>K</em>_a calculation reveal this pH-dependent regulation operates through protonation state change in the NUC-1 catalytic center, achieving maximal enzymatic activity at pH 6. Remarkably, this pH-gated regulatory mechanism is conserved across five nematode species spanning two distinct families, highlighting its broad biological significance and biotechnological potential. Our study establishes the first comprehensive environmental assessment framework for nematodes-mediated eARG degradation and elucidates a pH-gated regulation mechanism at the molecular level, providing a novel foundation for developing biotechnologies to control AR dissemination with spatiotemporal accuracy.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"15 8 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low ultraviolet dose with high efficiency: Synergistic coupling of far-UVC and ferrate(VI) for ultrafast and selective degradation of micropollutants","authors":"Wenxin Shi, Chi Zhang, He Zhao, Heli Tang, Yanan Liu, Bing Zhang","doi":"10.1016/j.watres.2025.123785","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123785","url":null,"abstract":"Enhancing the reactivity and yield of reactive species to reduce the ultraviolet (UV) fluence requirement for activating ferrate (Fe(VI)) is critical for advancing UV-based Fe(VI) processes toward practical wastewater treatment applications, yet it remains challenging. Herein, we developed a far-UVC-driven Fe(VI) activation system for the efficient degradation of micropollutants. The results demonstrated that switching from conventional low-pressure UV lamps (LPUV, UV₂₅₄) and UVA₃₆₅ to 222 nm far-UVC achieved ultrafast degradation of carbamazepine (CBZ) at an extremely low UV dose of 29.76 mJ/cm² under pH 8.0 conditions. The fluence-based degradation rate constants were 15.8 and 142.0 times higher than those achieved by UV₂₅₄ and UVA₃₆₅ photolysis of Fe(VI), respectively. This improved degradation can be attributed to the increased generation of high-valent iron intermediates [(Fe(V)/Fe(IV)] in the system. Notably, the presence of complex matrixes barely influenced CBZ degradation, and the UV₂₂₂/Fe(VI) system maintained significantly enhanced performance in various real waters compared to Fe(VI) alone. Additionally, 10 structurally diverse pollutants were selected for evaluation the selectivity of the UV₂₂₂/Fe(VI) system, finding that their ln<em>k_obs</em> values correlated well with their E_HOMO and vertical IP (<em>R</em>² = 0.86). Overall, this study proposes a promising oxidation technology that was efficient, energy-saving, cost-effective, and selective for the rapid elimination of micropollutants.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"1 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreased bromate formation but inadvertently increased toxicity in the chloramination-ozonation process: Essential roles of halamines in generating halogenated and nitrogenous byproducts","authors":"Ye Du, Xiao-Nan Wu, Bao-Jun Xu, Chang-Jie Yuan, Shu-Xi Guo, Yu-En Guo, Yao Lu, Qian-Yuan Wu","doi":"10.1016/j.watres.2025.123786","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123786","url":null,"abstract":"The prechloramination followed by post-ozonation (NH₂Cl-O₃) process has been widely recognized for its effectiveness in suppressing bromate (BrO₃^-) formation. However, the presence of bromide (Br^-) during NH₂Cl treatment results in the formation of various halamines. This study reveals that while the NH₂Cl-O₃ process reduces BrO₃^- formation, it leads to a substantial increase in overall cytotoxicity (from 2.01-4.10 to 4.30-12.05 mg-Phenol/L) and genotoxicity (from 0.29 to 0.88 µg-4-NQO/L) to mammalian cells at the condition of 3 mg/L NH₂Cl and 1 mg-O₃/mg-C. Total organic halogen, especially total organic bromine, markedly increases during the NH₂Cl-O₃ process, with TOBr rising from 7.2 μg/L in SE to 72.5 μg/L with 3 mg/L NH₂Cl, and to 75.7 μg/L with 5 mg/L NH₂Cl. By employing Ultra-Performance Liquid Chromatography-Orbitrap Mass Spectrometry (UPLC-Orbitrap MS) combined with halogenated isotopic feature detection and ¹⁵N-labeled isotope techniques, we precisely identified the molecular formulas of these disinfection byproducts (DBPs), demonstrating that the NH₂Cl-O₃ process promotes the formation of a broader range of both halogenated DBPs and nitrogenous byproducts (N-DBPs). In the presence of Br^-, after prechloramination, various halamines such as bromochloramine (NHBrCl), monobromamine (NH₂Br), and dibromamine (NHBr₂) were formed. Prechloramination inadvertently enhances a synergistic halamines/O₃ process, amplifying DBP formation. These halamines can all contribute to an increase in toxicity, particularly when combined with O₃ treatment. While effectively controlling BrO₃^-, our findings highlight the need to consider overall toxicity and evaluate the formation of other potentially toxic DBPs.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"13 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-resolution Mass Spectrometry Recognized Tetrabromobisphenol A bis (2,3-dibromo-2-methylpropyl ether) (TBBPA-DBMPE) as a Contaminant in Sediment from a Flame Retardant Manufacturing Factory","authors":"Hao Sun, Langjie Ye, Mengkai Yang, Guanyong Su","doi":"10.1016/j.watres.2025.123783","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123783","url":null,"abstract":"Information on contamination status of tetrabromobisphenol, and triazine-based novel brominated flame retardants (NBFRs) in sediment environment is very rare. Here, by use of high performance liquid chromatography coupled with quadrupole orbitrap mass spectrometry (HPLC-Q-Orbitrap/MS), we developed an analytical method for determination of three tetrabromobisphenol, and two triazine-based FRs in sediment samples. By applying this method for analysis of n=6 sediment samples from a flame retardant manufacturing factory, we observed that total concentrations of 6 NBFRs (∑₆NBFRs) ranged from 7.46 to 1020 ng/g dry weight (dw), which were comparable to those (3.83-820 ng/g dw) of n=11 sediment samples from e-waste recycling area. Both of them were statistically significantly (<em>p</em> &lt; 0.001, one-way ANOVA) higher than those in n=10 sediment samples from Taihu Lake (1.22-8.22 ng/g dw). With an aim to find novel tetrabromobisphenol, and triazine-based compounds, we further investigated the ionization characteristics and the fragmentation patterns in ionization source of six target NBFRs. We observed that debromination, ether bond breakage, and the breakage of inter-benzene ring C-C and C-S bonds were the main in-source fragmentation pathways for TBBPA/S derivatives. On the basis of the observed ionization characteristics and an in-house suspect screening list, we tentatively identified 71 NBFR formulas, of which ten TBBPA/S derivatives and one triazine-based BFRs were found for the first time in sediments. Among these NBFR formulas, tetrabromobisphenol A bis (2,3-dibromo-2-methylpropyl ether) (TBBPA-DBMPE) is high of concern due to its male reproductive toxicity according to a recent study, and fully confirmed by comparing unique LC and HRMS characteristics of sediment sample with authentic standard. The work probably provides an opportunity for the structural identification of unknown TBBPA/S derivatives in environmental samples, and reports the occurrence of TBBPA-DBMPE in real environment.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"46 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membranal phosphatidylglycerol enhances oxygen diffusion and release from cyanobacteria","authors":"Jian-Lu Duan, Yi Han, Xiao- Yu Liu, Mei-Yan Liu, Yu-Chen Sun, Jing-Ya Ma, Xiao-Dong Sun, Yue Wang, Miao-Miao Tan, Bo Gong, Xian-Zheng Yuan","doi":"10.1016/j.watres.2025.123782","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123782","url":null,"abstract":"Efficient oxygen transfer is critical challenge in algae-bacteria consortia, where aerobic bacteria depend on oxygen supplied by algae for the degradation of organic pollutants. Despite the well-documented role of cyanobacterial photosynthesis in oxygen production, the mechanisms regulating oxygen diffusion and release remain poorly understood. This study investigates the abiological functions of phosphatidylglycerol (PG), a key membrane phospholipid, in modulating oxygen dynamics in <em>Synechococcus elongates</em>. By engineering a PG-enriched <em>pgsA</em> mutant strain, we observed significantly enhanced oxygen diffusion and bubble release compared to the wild-type strain. Molecular dynamics simulations revealed that PG enrichment lowers energy barriers and increases the rate of oxygen permeation across the cell membrane. Single-cell adhesion measurements using atomic force microscopy demonstrated reduced cell-bubble adhesion forces in the <em>pgsA</em> strain, promoting efficient oxygen bubble detachment. PG incorporation also reduced surface roughness, decreased envelope stiffness, and enhanced membrane hydrophilicity, further supporting oxygen release. Importantly, PG enrichment did not affect photosynthetic efficiency or cell growth, indicating that the observed enhancements are driven by PG's abiological functions. These findings provide new insights into the role of membrane lipids in cyanobacterial oxygen dynamics and highlight PG's potential for improving oxygen delivery in environmental applications such as wastewater treatment and aquatic ecosystem restoration.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"13 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying assembly processes of dissolved organic matter pools in eutrophication using high-resolution mass spectrometry and ecological models","authors":"Guan-Lin Chen, Chen Qian, Meng Du, Min-Jie Tong, Jie-Jie Chen, Han-Qing Yu","doi":"10.1016/j.watres.2025.123781","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123781","url":null,"abstract":"Dissolved organic matter (DOM) represents a large, dynamic pool of carbon, playing a crucial role in eutrophic aquatic ecosystems through its continuous transport and transformation. However, the assembly mechanisms of DOM under different eutrophic conditions remain elusive, hindering the understanding of carbon dynamics and the prediction of carbon fate. Here we collected 72 lake water samples during two sampling events in Chaohu Lake, the fifth largest freshwater lake in China, and performed high-resolution mass spectrometry (HRMS) and ecological null modeling to quantify the assembly processes of DOM in eutrophication. We found that as eutrophic levels increased, the relative contribution of homogeneous selection rose, while the contributions of variable selection and dispersal limitation decreased. The influence of different assembly processes on the DOM pool across sites, although estimated solely from HRMS data, exhibited reasonable consistency with the spatiotemporal variations. Several environmental parameters, including total phosphorus, Secchi disk depth, trophic state index, pH, temperature, and fluorescence index, were significantly correlated with one or more DOM assembly processes (<em>p</em> &lt; 0.05), and assembly mechanisms also shaped the compound composition of DOM. Our findings reveal a shift in DOM assembly from variable selection to homogeneous selection in eutrophication, highlighting the importance of DOM dynamics and environmental homogenization in the management and restoration of eutrophic lakes.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"36 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic molecular network analysis reveals transformation signatures of dissolved organic matter during anaerobic digestion process","authors":"Xingsheng Yang, Xi Peng, Kai Feng, Shang Wang, Xiao Zou, Ye Deng","doi":"10.1016/j.watres.2025.123777","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123777","url":null,"abstract":"Identifying the transformation types, <em>i.e.,</em> syntheses or decompositions, of organic molecules in complex environmental systems remains a significant challenge. To address this, we propose a new analytical framework, Transformation-based Organic Molecular Ecological Network Analysis (TOMENA) for the systematic recognition and analysis of molecular transformations according to the measurement of high-resolution mass spectrometry (FT-ICR MS) through time-series data. Applying the TOMENA framework, we systematically investigated transformation signatures of dissolved organic matter (DOM) during anaerobic digestion processes. We found a close relationship between molecular transformation and molecular weight in the biodegradation system. A total of 129 transformations were identified, involving carbon numbers ranging from 0 to 24, with 59 of these transformations concentrated in small molecular weight changes involving 1-3 carbons. As the molecular weight corresponding to transformations increased, the proportion of bio-transformations used for decomposition decreased linearly. Simultaneously, large molecules were decomposed and small molecules synthesized, indicating a system tendency to transform molecules towards a medium mass range. Topological analysis of the transformation network further expanded our understanding. We discovered that molecular transformations did not follow the shortest path, as the path distance was significantly longer than in random networks (2.558 vs. 2.383). We identified that N-containing transformations were centrally located in the system through edge analysis. However, the transformations’ position did not coincide with functional importance. A comprehensive indicator of irreplaceability and usage frequency revealed that C(+1)H(+3)O(+2)N(-1), C(+1)H(+2), O(+1), C(+3)H(+4)O(+2), and H(-2)O(+1) are critical transformation pathways in the system, showing the top 5 efficiency contributions. Our developed TOMENA workflow provides novel insights and robust methodological support for future research, advancing our understanding of molecular transformations in complex biodegradation system.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"46 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial advanced oxidation aroused by bacteria-algae symbiosis induced abiotic methane production in anaerobic digestion","authors":"Jinshuo Zhang, Xuepeng Wang, Zhenglin Chen, Qilin Yu, Yaobin Zhang","doi":"10.1016/j.watres.2025.123776","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123776","url":null,"abstract":"The slow decomposition of recalcitrant substrate limits the conversion efficiency of anaerobic digestion. Microbial advanced oxidation, capable of in-situ generating reactive oxygen species (ROS) with the microbial aerobic/anaerobic respiration, provided a potential way to strengthen the substrate-methane conversion in anaerobic digestion. In this study, microalgae were inoculated in anaerobic system and formed redox oscillation under the intermittent illumination, which ultimately increased the methane production by 27.4%. With the redox oscillation, •OH, the typical ROS, showed a 6.27-fold increase in production (72.95 ± 9.06 μM vs. 10.03 ± 1.49 μM), facilitating the decomposition of lignocelluloses. Notably, abiotic methanation was observed in anaerobic digestion with the occurrence of microbial advanced oxidation. ROS quenching experiments revealed that abiotic methanation roughly accounted for 17.5% of the total methane production. Microbial advanced oxidation formed by redox oscillation showed the potential to strengthen anaerobic digestion. Notably, for the first time, it was confirmed that abiotic methanation could be established in anaerobic digestion with the ROS generated by microbial advanced oxidation, which offered a new perspective to understand and improve the performances of natural and engineered ecosystems.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"22 1","pages":"123776"},"PeriodicalIF":12.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}