Water ResearchPub Date : 2025-04-05DOI: 10.1016/j.watres.2025.123529
Rui Zhang, Sixin Zhang, Chunrui Li, Bin Cui, Dandan Zhou
{"title":"Transboundary intercellular communication mechanisms in the treatment of polyvinyl alcohol (PVA) wastewater by Geotrichum candidume enhanced activated sludge","authors":"Rui Zhang, Sixin Zhang, Chunrui Li, Bin Cui, Dandan Zhou","doi":"10.1016/j.watres.2025.123529","DOIUrl":"10.1016/j.watres.2025.123529","url":null,"abstract":"<div><div>Polyvinyl alcohol (PVA) wastewater is characterized by low biochemistry and poor biodegradability. Transboundary intercellular communication between fungi and bacteria modulates microbial metabolism activity. This mechanism facilitates the biological treatment of PVA wastewater potentially. In this study, a <em>Geotrichum candidume</em> enhanced activated sludge system was developed to treat PVA wastewater. The transboundary intercellular communication mechanism among fungi and bacteria was elucidated, and the promotion of PVA removal was studied. Results showed that <em>G. geotrichum</em> sensed the N-decanoyl-<span>l</span>-homoserine lactone (C10-HSL) secreted by <em>Acidovorax</em>, leading to the upregulation of functional genes (<em>HAO, LACC1, FAHD</em>) for PVA degradation. Dehydrogenase (DHA) and laccase activities were increased by 15.2 %-38.6 % and 77.1 %-114.5 % after C10-HSL addition. Meanwhile, C10-HSL enhanced metabolic processes such as tricarboxylic acid cycle (TCA cycle), and oxidative phosphorylation in <em>G. geotrichum</em> to accelerate PVA degradation. As a result, <em>G. geotrichum</em> biodegraded PVA into aldehydes and ketones, which were further mineralized by bacteria such as <em>Dechloromonas</em>. For the co-culture system, the synergistic interaction between <em>G. geotrichum</em> and functional bacteria significantly enhanced PVA degradation via the intercellular communication process. The PVA and chemical oxygen demand (COD) removal increased by 31.7 % and 28.3 %, respectively, compared to the activated sludge system. This study offers a novel theoretical foundation and approach for the biological treatment of PVA wastewater through fungal-bacterial transboundary intercellular communication.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"281 ","pages":"Article 123529"},"PeriodicalIF":11.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-05DOI: 10.1016/j.watres.2025.123602
Rui Gao, Shu-Hong Gao, Jun Li, Fang Huang, Yanmei Zhao, Jingni Xie, Yusheng Pan, Wanying Zhang, Aijie Wang
{"title":"Removal of disinfection residual bacteria in UV222, UV222/H2O2 and UV222/peroxymonosulfate systems: what is the safe usage for wastewater reclamation","authors":"Rui Gao, Shu-Hong Gao, Jun Li, Fang Huang, Yanmei Zhao, Jingni Xie, Yusheng Pan, Wanying Zhang, Aijie Wang","doi":"10.1016/j.watres.2025.123602","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123602","url":null,"abstract":"Disinfection residual bacteria (DRB) are widely present in the reclaimed treatment effluents and can regrow during the downstream distribution and storage, posing a threat to the biosafety of reuse applications. Recently, far ultraviolet (UV<sub>222</sub>) have garnered augmented attention due to the highly efficient and energy-intensive oxidation, making them a potential approach for the deep inactivation of DRB. However, there remains a lack of quantitative analyses on how to monitor the disinfection intensity to mitigate the health risks associated with DRB. In this study, we used the UV<sub>222</sub>, UV<sub>222</sub>/H₂O₂ and UV<sub>222</sub>/peroxymonosulfate (PMS) systems to treat model DRB including <em>Escherichia coli, Pseudomonas aeruginosa</em>, and <em>Bacillus subtilis</em>, and developed a multiparameter model to accurately present the dose-culturability relationship. On this basis, we conducted the simulated disinfection, and detected the viability status and regrowth potential of DRB during the post-disinfection processes. It turned out that UV<sub>222</sub> alone exhibited the superiority over UV<sub>254</sub>, especially for treating <em>Pseudomonas aeruginosa</em>. UV<sub>222</sub>/H<sub>2</sub>O<sub>2</sub> and UV<sub>222</sub>/PMS systems further improved the inactivation rates. The practical UV doses for full-scale reclaimed disinfection (10–200 mJ/cm²) were sufficient for the UV<sub>222</sub>-based systems to inactivate DRB (initial 10<sup>7</sup> CFU/mL) to the safe level in effluent measured by culture methods. But substantial DRB still persisted in VBNC state, which necessitated higher doses of 200–450 mJ/cm<sup>2</sup> to further inhibit the regrowth under accidental contamination and prolonged transport/storage culture. Fortunately, H<sub>2</sub>O<sub>2</sub> provided residual disinfection for <em>Bacillus subtilis</em>, and PMS performed promising sustained disinfection for all the three DRB. This study provided valuable insights for the expanded application of UV<sub>222</sub> disinfection and future updates of pathogen standards in reclaimed water treatment.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"73 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-05DOI: 10.1016/j.watres.2025.123599
Jun Li, Lili Li, Weiqiang Liang, Lingyu Li, Ruya Wang, Zhenhua Wang, Chunxia Ma
{"title":"Spatial multi-omics analysis of metabolic heterogeneity in zebrafish exposed to microcystin-LR and its disinfection byproducts","authors":"Jun Li, Lili Li, Weiqiang Liang, Lingyu Li, Ruya Wang, Zhenhua Wang, Chunxia Ma","doi":"10.1016/j.watres.2025.123599","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123599","url":null,"abstract":"Most studies on the biological effects of exogenous pollutants have focused on whole samples or cell populations, and lack spatial heterogeneity consideration due to technical limitations. Microcystin-LR (MC-LR) from cyanobacterial blooms threatens ecosystems and human health, while microcystin-LR disinfection by-products (MCLR-DBPs) in drinking water remain a concern for their toxin-like structure. This study introduces spatial multi-omics to investigate the disruptions caused by ingestion of MC-LR and MCLR-DBPs in zebrafish. The method integrates metabolomics, spatial metabolomics, and spatial transcriptomics to characterize the overall metabolic changes in whole zebrafish caused by MC-LR and MCLR-DBPs, then provides further insight into the variation of spatial distribution of metabolites and genes in MC-LR and MCLR-DBPs targeted organ. The results showed that MC-LR and MCLR-DBPs induced oxidative stress and metabolic imbalance, and disrupted the physiological homeostasis of zebrafish. Spatial multi-omics analysis further revealed that MC-LR and MCLR-DBPs exacerbate disruptions in energy and lipid metabolism, methylation processes, and immune pathways by modulating the expression of genes such as <em>gatm, gnmt, cyp2p9</em>, and <em>tdo2b</em>. In conclusion, this study developed a spatial multi-omics approach that not only enhances the understanding of the biological effects of MC-LR and MCLR-DBPs but also provides robust technical support for investigating other environmental pollutants.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"4 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-05DOI: 10.1016/j.watres.2025.123603
Margaret E. Knight, Kata Farkas, Adriana Kiss, Davey L. Jones
{"title":"National-Scale Insights into AMR Transmission Along the Wastewater-Environment Continuum","authors":"Margaret E. Knight, Kata Farkas, Adriana Kiss, Davey L. Jones","doi":"10.1016/j.watres.2025.123603","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123603","url":null,"abstract":"The circulation of antimicrobial resistance (AMR) bacteria between human populations and the environment is a key driver of the global AMR burden, with wastewater acting as a major route of transmission. In this nationwide study, influent and effluent samples were collected from 47 municipal wastewater treatment plants (WWTPs) across Wales, covering areas of varying sociodemographics and representing approximately 66% of the population connected to the main sewer network. Additionally, 76 river and estuarine sediment samples were collected upstream and downstream of the WWTPs, as well as from nearby recreational beaches. High-throughput qPCR was used to quantify 76 antimicrobial resistance genes (ARGs), 10 mobile genetic elements and 5 pathogens. Our analyses revealed that the absolute abundance and composition of the influent resistome was influenced by increasing WWTP catchment population size and density. Significant shifts in the resistome were observed following the wastewater treatment process, with the biological treatment stage identified as a critical determinant of AMR removal efficiency. WWTPs using biological filter beds were found to be more effective in reducing ARG relative abundances compared to those employing activated sludge processes. Despite the presence of ARGs in the effluent, the abundance and diversity of the river sediment resistomes did not increase downstream of the WWTPs. However, the presence of a resistome was found in all sediment samples, with varying compositions influenced by WWTP size and sediment source. Altogether, these findings highlight the complex and interconnected factors that shape the resistome across the wastewater-environment continuum, highlighting the need for comprehensive, nationwide surveillance studies to inform targeted interventions and mitigate the spread of AMR.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"40 3 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-04DOI: 10.1016/j.watres.2025.123534
Mingqi Guo, Stefan A.F. Bon, Soroush Abolfathi
{"title":"Transport dynamics of microplastics within aquatic vegetation featuring realistic plant morphology","authors":"Mingqi Guo, Stefan A.F. Bon, Soroush Abolfathi","doi":"10.1016/j.watres.2025.123534","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123534","url":null,"abstract":"Despite the significance of rivers and streams in transporting terrestrial microplastics (MP) to the oceans, limited research has focused on the role of aquatic vegetation and their complex geometry in shaping the underlying mechanisms governing MP mixing and dispersion processes in riverine environments. This study, for the first time, investigates the transport and fate of non-buoyant MPs, specifically those with diameters of 188 nm and <span><math><mrow is=\"true\"><mn is=\"true\">6</mn><mspace is=\"true\" width=\"1em\"></mspace><mi is=\"true\" mathvariant=\"normal\">μ</mi><mi is=\"true\" mathvariant=\"normal\">m</mi></mrow></math></span> and a density of 1.04 g/cm<span><math><msup is=\"true\"><mrow is=\"true\"></mrow><mrow is=\"true\"><mn is=\"true\">3</mn></mrow></msup></math></span>, in floating <em>Eichhornia crassipes</em> canopies under flow conditions typical of natural rivers (0.0167-0.0667 m/s). Physical modelling tests reveal that aquatic vegetation significantly alters the hydrodynamic structure and enhances the dissipation of turbulence in the water column, leading to decreased velocities, diversified length scales, and increased turbulent kinetic energy (TKE) in regions with higher frontal vegetation areas. This turbulence, in turn, facilitated momentum exchange and vertical mixing, particularly in regions with the most pronounced frontal area changes. Wider canopy spacing promoted the evolution of wake turbulence and facilitated wake expansion throughout the water column, generating coherent structures that effectively doubled the integral length scales with increasing distance between canopies from 0.5 m to 1.5 m. This adjustment resulted in a more uniformly dispersed downstream movement of MPs. Notably, the presence of canopies amplified MP diffusivity by 10-40 times compared to equivalent unvegetated conditions, transitioning the primary mixing mechanism from shear-induced velocity gradients to turbulence enhanced by plant-flow interactions. This study offers a robust framework for quantifying MP mixing and predicting longitudinal dispersion coefficients within the floating vegetated flows, by developing models that depict the vertical profiles of TKE and turbulent diffusivity featured by canopy morphology and spacing. The insights from this study make a significant contribution toward improving our ability to predict the mixing and fate of MPs in riverine environments and underscore the necessity of incorporating the complex dynamics of aquatic vegetation into environmental management and MP risk assessments.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"37 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-04DOI: 10.1016/j.watres.2025.123584
Clara Serrano, Katerina Savva, Maria Fernández-Altimira, Marinella Farré, Maria Vila-Costa, Marta Llorca
{"title":"Effects of bioplastics and their leachates on marine bacterial communities","authors":"Clara Serrano, Katerina Savva, Maria Fernández-Altimira, Marinella Farré, Maria Vila-Costa, Marta Llorca","doi":"10.1016/j.watres.2025.123584","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123584","url":null,"abstract":"Biobased biodegradable plastics (bioplastics) are promising alternatives fuel-based plastics. However, higher additive content is generally used to perform similarly to fuel-based materials. Moreover, plastic additives are not covalently bound to carbon chains and can leach into aquatic environments such as seawater, potentially impacting marine biota, though the extent of these effects is not yet fully understood. To evaluate the leaching behaviour in seawater of plastic additives from bioplastics materials and its impact on coastal surface marine bacterial communities, microcosm experiments were conducted using seawater amended with non-aged pellets of polylactic acid (PLA), poly-hydroxybutyrate (PHB), and commercial materials, a knife of PLA and a bag of PLA&PHB, under biotic and abiotic conditions for two months. Samples were taken weekly to study the bacterial abundance, bi-weekly to evaluate the leaching process and potential biodegradation products and, finally, at the end of the experiment to study the microbial communities. Chemical analysis was performed using suspect screening by means of Liquid Chromatography coupled to High-Resolution Mass Spectrometry (LC-HRMS) equipped with Electrospray Ionization source working in positive and negative conditions. The experiment showed that 177 compounds were tentatively identified at confidence level 2, including plasticizers, UV filters, flame retardants, and PLA- and PHB-related degradation products, which were detected in similar proportions under abiotic and biotic processes. Bacterial communities exposed to PLA showed higher production and significant differences in the community composition, with PLA degraders being identified in the different plastispheres studied. Leachates impacted differently microbial communities in the free-living fraction, particle-attached fraction and in the plastisphere, indicating taxa-specific responses. To the best of our knowledge, this is the first study providing further insights into the influence of bioplastics and their leachates on marine microbial communities, contributing to our understanding of bioplastics’ effects on the global oceans.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"23 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-04DOI: 10.1016/j.watres.2025.123595
Jinqiang Guo, Bu Zhou, Eric P. Achterberg, Yuan Shen, Jinming Song, Liqin Duan, Xuegang Li, Huamao Yuan
{"title":"Enhanced carbon sequestration in marginal seas through bacterial transformation","authors":"Jinqiang Guo, Bu Zhou, Eric P. Achterberg, Yuan Shen, Jinming Song, Liqin Duan, Xuegang Li, Huamao Yuan","doi":"10.1016/j.watres.2025.123595","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123595","url":null,"abstract":"Labile organic carbon is a highly dynamic component of the marine carbon pool, traditionally thought to be respired within hours to days into carbon dioxide (CO<sub>2</sub>) by bacteria, although there is a paucity of direct observational evidence. Here, we report that a significant portion of labile particulate organic carbon (POC) in marginal seas is converted into bacterial material. By exploiting D/L-amino acids, we trace the origins of labile POC and its transformation into bacterial POC in the marginal seas off Eastern China. Our results indicate that labile POC primarily originates from autochthonous primary production, with bacterial POC fractions closely paralleling those of labile POC. It appears that rapid bacterial POC transformation is driven by enhanced bacterial growth efficiency from abundant nutrients in marginal seas. We estimate that around 0.08 ± 0.03 Pg of bacterial organic carbon is buried annually in global marginal seas, accounting for ∼40% of total organic carbon burial, thus contributing to long-term carbon sequestration. These findings highlight the critical role of bacterial transformation in carbon sequestration within marginal seas and provide a potential mechanism for the observed increase in CO<sub>2</sub> uptake in coastal regions.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"63 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-03DOI: 10.1016/j.watres.2025.123554
Heli Wang , Yin Zhong , Qian Yang , Jiaying Li , Dan Li , Junhong Wu , Sen Yang , Jiashuo Liu , Yirong Deng , Jianzhong Song , Ping'an Peng
{"title":"Erratum to “Coupling of Sulfate Reduction and Dissolved Organic Carbon Degradation Accelerated by Microplastics in Blue Carbon Ecosystems” [Water Research, 279, (2025), 123414]","authors":"Heli Wang , Yin Zhong , Qian Yang , Jiaying Li , Dan Li , Junhong Wu , Sen Yang , Jiashuo Liu , Yirong Deng , Jianzhong Song , Ping'an Peng","doi":"10.1016/j.watres.2025.123554","DOIUrl":"10.1016/j.watres.2025.123554","url":null,"abstract":"","PeriodicalId":443,"journal":{"name":"Water Research","volume":"281 ","pages":"Article 123554"},"PeriodicalIF":11.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-02DOI: 10.1016/j.watres.2025.123577
Jiayu Bi, Ricardo Marques, Dongqi Wang, Lu Qin, Kylie Close, Guangyu Li, Zijian Leo Wang, Nicholas B. Tooker, Varun Srinivasan, Annalisa Onnis-Hayden, Adrian Oehmen, April Z. Gu
{"title":"Phenotypic Discrimination and Characterization of Microbial Populations in Enhanced Biological Phosphorus Removal Using Single-Cell Raman Spectroscopy-Based Methods","authors":"Jiayu Bi, Ricardo Marques, Dongqi Wang, Lu Qin, Kylie Close, Guangyu Li, Zijian Leo Wang, Nicholas B. Tooker, Varun Srinivasan, Annalisa Onnis-Hayden, Adrian Oehmen, April Z. Gu","doi":"10.1016/j.watres.2025.123577","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123577","url":null,"abstract":"Single-cell Raman spectroscopy (SCRS) represents a non-invasive, expedient, and label-free strategy for investigating the molecular composition of individual cells. In this study, we applied SCRS to perform qualitative and quantitative analyses of polyphosphate (polyP) accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) within enhanced biological phosphorus removal (EBPR) systems, enabling their metabolic trait-based profiling and phenotypic classification. SCRS analysis revealed diverse metabolic profiles of metabolically active EBPR populations including unknown GAOs and PAOs performing GAO metabolism. The dynamics of intracellular polymers quantified by SCRS were highly correlated with bulk measurements, while also providing additional metabolic information. SCRS analysis, combined with carbon feeding batch tests and hierarchical clustering analysis (HCA), could phenotypically classify clade-level PAO/GAO subpopulations with distinct carbon metabolisms and Raman spectral features (e.g., shift in signature peak, whole fingerprint region). The combination of fluorescence <em>in situ</em> hybridization (FISH) with Raman (FISH–Raman) and HCA, for the first time, revealed higher phenotypic microdiversity for <em>Tetrasphaera</em> and substantial differences in polyP peak position between <em>Tetrasphaera</em> and <em>Accumulibacter</em> cells. <em>Tetrasphaera</em> PG1, characterized by high polyP content and potentially belonging to members of clade 2 or 3, was identified as a primary contributor in a side-stream EBPR system. These findings offer novel insights into the metabolic processes and growth dynamics of microorganisms within EBPR systems, providing a critical tool for deciphering the microdiversity and metabolic behaviors of PAO/GAO populations in complex EBPR communities.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"73 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water ResearchPub Date : 2025-04-02DOI: 10.1016/j.watres.2025.123592
Yunxia Zu, Zhiling Li, Zimeng Zhang, Xueqi Chen, Bin Wu, Shih-Hsin Ho, Aijie Wang
{"title":"Iron-reduction driven extracellular electron transfer widely promotes microbial reductive dechlorination metabolism","authors":"Yunxia Zu, Zhiling Li, Zimeng Zhang, Xueqi Chen, Bin Wu, Shih-Hsin Ho, Aijie Wang","doi":"10.1016/j.watres.2025.123592","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123592","url":null,"abstract":"Biological iron reduction and reductive dehalogenation occur in similar ecological environments, however, how Fe(III)/Fe(II) redox cycles impact the microbial dehalogenation processes remains controversial. In this study, the favorable microbial reductive dechlorination activity has been widely observed in iron-rich river sediments by national sampling, with the dechlorination efficiency showing a positive correlation with the concentration of Fe(III). Microcosm experiments demonstrated that the addition of nano-hematite resulted in a maximum increase of 2.16 times in the dechlorination rate constant (<em>k</em>) for 2,4,6-trichlorophenol, achieved <em>via</em> synergistic interactions with Fe(III) reduction. Multi-tools, including transcriptomic analyses, revealed that the addition of nano-hematite enhanced the process of Fe(III) reduction by upregulating genes associated with extracellular electron transfer (e.g., <em>CYC, pliM</em>) and conductive biofilm formation (e.g., <em>livH, secY, wza</em>). This synergistic Fe(III) reduction further facilitated intracellular carbon metabolism, energy production, and reductive dechlorination, as confirmed by the upregulated functional genes identified through transcriptomics and RT-<em>q</em>PCR. The discovery of the novel phenomenon involving synergistic Fe(III) reduction and dehalogenation broadens our understanding of the biochemical cycling of organohalides (e.g., chlorinated phenols) in iron-rich environment, and provides a feasible strategy for improving biodehalogenation through the regulation of carbon and electron flow at sites contaminated with organohalides.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"23 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}