Trends in Analytical Chemistry最新文献

{"title":"CRISPR-assisted bioanalytical sensing of cytokines","authors":"Smriti Gaba ,&nbsp;Fei Deng ,&nbsp;Utkarsh Jain","doi":"10.1016/j.trac.2025.118318","DOIUrl":"10.1016/j.trac.2025.118318","url":null,"abstract":"<div><div>A vital component of innate and adaptive inflammatory host defenses is a soluble extracellular protein called cytokine, which regulates cell differentiation, growth, angiogenesis, and cell death, as well as development and repair processes that assist in re-establishing homeostasis. Cytokines, biomarkers for many diseases, are highly valuable across biology and clinical medicine. Their quantification can help with diagnosis and therapy by offering insights into pathological and physiological processes. Due to complicated cytokine networks and low concentration, cytokines are difficult to measure. Prominent conventional immunoassays require multiple steps for the loading of antibodies and samples, which makes detection laborious and time-consuming. Also, the sensitivity is low due to a lack of inherent signal amplification. The emergence of advanced multifarious technology like Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) may be able to meet the unmet demands of real-time, quick, and highly sensitive detection. CRISPR-Cas technology is a new approach to biosensing that is widely recognized for its exceptional precision. Recently, CRISPR-based diagnostic systems have undergone an advancement from genome editing to a clinically applicable diagnostic method for the rapid and highly sensitive identification of biomarkers, including DNAs/RNAs (circulating tumor DNAs or microRNAs), Ochratoxin A, proteins including calprotectin, and cytokines. Early diagnosis and prevention of chronic diseases can be greatly improved by the comprehensive characterization and timely analysis of cytokines using CRISPR-Cas technology. Since the finding of distinct endonuclease activity, this technology has significantly advanced the development of bioanalytical sensors. In particular, sophisticated disease diagnostic systems are developed using the multiplexed sensing capacities of CRISPR to address critical health problems. The following review explores the analytical tools and techniques that have been built on CRISPR-Cas technology for molecular detection, especially cytokines. It emphasizes the advantages, challenges, engineering strategies, and technological advancements of CRISPR-Cas systems in biosensing, intending to stimulate innovative concepts to tackle practical issues.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"192 ","pages":"Article 118318"},"PeriodicalIF":11.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inductively coupled plasma mass spectrometry-based immunoassay: An update from 2015 to 2025","authors":"Min Jiang ,&nbsp;Chengchao Zhang ,&nbsp;Yanlin Chen ,&nbsp;Mingxia Sun ,&nbsp;Rui Liu ,&nbsp;Yi Lv","doi":"10.1016/j.trac.2025.118317","DOIUrl":"10.1016/j.trac.2025.118317","url":null,"abstract":"<div><div>Inductively coupled plasma mass spectrometry (ICP-MS) has emerged as an important analytical platform in the field of biomarker detection, leveraging its exceptional sensitivity and resolution. Over the past decade, ICP-MS-based immunoassays have experienced rapid development, not only overcoming the sensitivity and multiplexing limitations of traditional optical methods but also achieving extensive applications in clinical scenarios. Elemental tags, as a core technological module of ICP-MS bioassays, serve as the critical bridge connecting biomolecular recognition and mass spectrometric signal output. Their design and performance directly influence the sensitivity, multiplexity, and throughput of the detection. Therefore, the focus of this review will be mainly on elemental tags, summarizing the latest advancements in metal ions, nanoparticles, and label-free strategies while exploring how these innovations enable ICP-MS bioassays to achieve high sensitivity, multiplexity, and high-throughput analysis. Additionally, this review also covers related bioassays based on similar principles, such as nucleic acid hybridization and aptamer-based assays.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118317"},"PeriodicalIF":11.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent developments and workflows of non-targeted LC-HRMS screening in the detection of veterinary drug residues in foods","authors":"Yishuang Yang ,&nbsp;Jiaxi Chen ,&nbsp;Guiyuan Li ,&nbsp;Anastasios Koidis ,&nbsp;Zhongping Yao ,&nbsp;Hongtao Lei ,&nbsp;Xiaoqun Wei","doi":"10.1016/j.trac.2025.118314","DOIUrl":"10.1016/j.trac.2025.118314","url":null,"abstract":"<div><div>Monitoring veterinary drug residues in food is one key link to ensuring food safety. In recent years, non-targeted screening technology based on liquid chromatography-high resolution mass spectrometry (LC-HRMS) has attracted widespread attention. However, the development and application of non-targeted screening methods still face many challenges due to the constantly updated variety of veterinary drugs, the complexity of sample matrices, the large amount of data, and the cumbersome data identification process. To address the above challenges, this review systematically proposes a workflow for non-targeted screening, focusing on the research progress in sample preparation, instrument analysis, data preprocessing, and recognition strategy. In addition, it summarizes molecular formula prediction models, chemical structure prediction models, and retention time validation models, discusses the latest application of this technique in detecting veterinary drug residues in food, providing new insights to meet the challenges of non-targeted screening.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118314"},"PeriodicalIF":11.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering signal interfaces for enhanced electrochemical biosensing","authors":"Masoud Negahdary ,&nbsp;Mayank Garg ,&nbsp;Gerard L. Coté ,&nbsp;Leyla Soleymani ,&nbsp;Mahla Poudineh ,&nbsp;Samuel Mabbott","doi":"10.1016/j.trac.2025.118310","DOIUrl":"10.1016/j.trac.2025.118310","url":null,"abstract":"<div><div>Signal interface design advances have revolutionized electrochemical biosensing by greatly enhancing electron transfer efficiency, signal stability, and analyte selectivity. Recent innovations, including 3D-printed sensing platforms, soft and stretchable electrodes, and laser-induced graphene (LIG) interfaces, have enabled biosensors with heightened sensitivity, reduced interference, and improved durability. Merging high-performance surface engineering and embedded technologies like microfluidics has opened avenues toward improved functionality for convenient operation across wide physiological conditions. Such advances have been particularly impactful in biomarker detection, where signal-optimized interfaces allow ultrasensitive real-time monitoring of clinically relevant analytes in biofluids such as sweat and interstitial fluid (ISF). This development lays the groundwork for the next generations of non-invasive health monitoring tools by bridging the gap between traditional laboratory diagnostics and novel point-of-care systems. This review offers a snapshot of signal interface innovations, categorizing recent work from the past two years into key thematic areas. We emphasize emerging trends in electrode functionalization, transduction amplification, and interface engineering, with a critical analysis of how these translate into the enhancement of biosensor performance. Providing a snapshot of the state-of-the-art electrochemical biosensing, this review is a reference guide for researchers developing novel biosensing platforms.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118310"},"PeriodicalIF":11.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in nucleic acids and non-nucleic acids nanostructures-enhanced site-specific DNA methylation biosensing","authors":"Yongchao Yao ,&nbsp;Limei Zhang ,&nbsp;Xun He ,&nbsp;Lianhua Liu ,&nbsp;Peilin Wu ,&nbsp;Mei Yang ,&nbsp;Zhengwei Cai ,&nbsp;Hong Tang ,&nbsp;Hao Bai ,&nbsp;Yi Li ,&nbsp;Binwu Ying ,&nbsp;Xuping Sun ,&nbsp;Wenchuang Walter Hu","doi":"10.1016/j.trac.2025.118315","DOIUrl":"10.1016/j.trac.2025.118315","url":null,"abstract":"<div><div>The early detection of diseases, leveraging DNA methylation as a crucial biomarker, has seen remarkable progress through the integration of nucleic acids and non-nucleic acids nanostructures in biosensing platforms. This review explores how nucleic acids-based nanostructures, such as DNA tetrahedra and G-quadruplexes, alongside diverse non-nucleic acids nanomaterials like metal nanoparticles, carbon-based nanomaterials, and nanopores, are propelling advancements in sensitivity, specificity, and analytical depth for site-specific DNA methylation analysis. With a focus on key trends in signal amplification and enhanced specificity, we examine the evolving role of nanostructures in addressing the challenges of methylation profiling within complex biological environments. Through a comprehensive analysis of recent developments, this review showcases how these developments pave the way for major enhancements in precision diagnostics, thereby expanding the efficacy and accuracy of DNA methylation-based biosensing approaches for the early detection of diseases.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118315"},"PeriodicalIF":11.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144154932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable analytical methods for detecting synthetic cannabinoid receptor agonists: a critical review","authors":"Larissa M.A. Melo ,&nbsp;Lívia M.S. Aranha ,&nbsp;Cecília N.F. Barroso ,&nbsp;Luciano C. Arantes ,&nbsp;Rodrigo A.A. Muñoz ,&nbsp;Wallans T.P. dos Santos","doi":"10.1016/j.trac.2025.118311","DOIUrl":"10.1016/j.trac.2025.118311","url":null,"abstract":"<div><div>Synthetic Cannabinoid Receptor Agonists (SCRAs) are a chemically diverse class of psychoactive substances that activate cannabinoid receptors and mimic the pharmacological effects of delta-9-tetrahydrocannabinol, despite often having markedly different chemical structures. Given the vast array of chemical structures within this drug class, coupled with the rapid introduction of new variants, detecting SCRAs has become a challenge in forensic studies. While traditional methods like chromatography with mass spectrometry offer high accuracy, they require expensive, bulky, and complex equipment. This has made the exploration of portable detection methods particularly attractive. This review focuses on the development of innovative, rapid, and straightforward methods for identifying SCRAs-type substances, including electrochemistry, electrochemiluminescence, quartz-crystal microbalance, fluorescence, surface-enhanced Raman scattering and infrared spectroscopy. We compare their analytical performance in terms of detection limits, quantification ranges, and applicable sample matrices. Finally, we discuss current limitations and outline emerging trends that guide future developments in portable SCRAs detection technologies.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118311"},"PeriodicalIF":11.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in online derivatization for liquid chromatography-mass spectrometry: Automation and performance enhancement","authors":"Fangfang Wang ,&nbsp;Lihua Zuo ,&nbsp;Mengyuan Lv ,&nbsp;Yuyang Wang ,&nbsp;Ruobing Ren ,&nbsp;Di Chen ,&nbsp;Zhi Sun","doi":"10.1016/j.trac.2025.118300","DOIUrl":"10.1016/j.trac.2025.118300","url":null,"abstract":"<div><div>Online derivatization coupled with liquid chromatography-mass spectrometry (LC-MS) has become a powerful method for analyzing target compounds in complex samples. It improves detectability, separation efficiency, sensitivity, selectivity, and overall data quality, particularly for weakly ionizable analytes. By automating sample pretreatment and analysis, it increases throughput, reproducibility, and reduces time, labor, and costs. Various online derivatization strategies, including pre-column, on-column, and post-column methods, have been developed, each with unique configurations and applications. Despite significant research, existing reviews typically focus on specific aspects like offline derivatization or reagents, leaving gaps in comprehensive coverage. This article provides an overview of recent online derivatization approaches, discussing their advantages, disadvantages, applications, and offering guidance for selecting the most suitable techniques for different scenarios.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118300"},"PeriodicalIF":11.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"State of the art and perspectives of improving antibody performance in food immunosensors","authors":"Haoran Xiao ,&nbsp;Shiwen Han ,&nbsp;Mingzhe Zhu ,&nbsp;Mingxia Lin ,&nbsp;Nan Cheng ,&nbsp;Huilian Che","doi":"10.1016/j.trac.2025.118308","DOIUrl":"10.1016/j.trac.2025.118308","url":null,"abstract":"<div><div>Immunosensors have become a rapid, cost-effective, and sensitive tool for food safety detection, with antibodies serving as essential biorecognition components. However, challenges like low specificity, time-consuming preparation, animal dependency, and high costs limit their application. Recent studies highlight strategies for improving antibody performance, including rational hapten design, optimized conjugation techniques, and spacer arm length optimization. Computer-aided molecular modeling can validate designs before the immunization step, ensuring design efficacy. Phage display enables recombinant antibody production, allowing efficient preparation of antibodies from various phage antibody libraries through tailored screening strategies. Additionally, modifications to the ligands and formats used in chromatography can facilitate the development of high-throughput, cost-effective antibody purification methods. This review also offers a comprehensive overview of the type of immunosensors used in food safety, focusing on optical, electrochemical and multi-mode immunosensors. The goal is to offer new insights and approaches to enhance antibody production efficiency and their use in immunosensors.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118308"},"PeriodicalIF":11.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144138242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of oligonucleotide mapping with liquid chromatography – mass spectrometry to study the primary structure of mRNA","authors":"Jonathan Maurer ,&nbsp;Helena Vanluchene ,&nbsp;Athanasios Tsalmpouris ,&nbsp;Kris Morreel ,&nbsp;Julien Camperi ,&nbsp;Koen Sandra ,&nbsp;Davy Guillarme","doi":"10.1016/j.trac.2025.118309","DOIUrl":"10.1016/j.trac.2025.118309","url":null,"abstract":"<div><div>Oligonucleotide (ON) or RNA mapping using liquid chromatography coupled to mass spectrometry (LC-MS) has recently emerged as an attractive approach to support the development of nucleic acid-based medicines, in particular mRNA. This technique provides valuable information on the primary structure of mRNA by combining enzymatic digestion, high-resolution chromatographic separation, accurate mass determination, optimized fragmentation strategies and advanced data treatment, similar to the peptide mapping workflows commonly employed for structural elucidation and quality assessment of therapeutic proteins.</div><div>This review critically examines the latest developments in enzymatic digestion methods, liquid chromatography techniques, mass spectrometry conditions, and data treatment strategies to improve the performance of ON mapping and also provides some perspectives to further optimize this workflow.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118309"},"PeriodicalIF":11.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Materials for 3D printing of chromatographic stationary phases","authors":"Mariachiara Conti ,&nbsp;Simone Dimartino","doi":"10.1016/j.trac.2025.118301","DOIUrl":"10.1016/j.trac.2025.118301","url":null,"abstract":"<div><div>Stationary phases fabricated using 3D printing hold the promise of enhancing chromatography performance, offering bespoke solutions with unparalleled reproducibility and precise control over geometrical features. However, the limited array of printable materials available and constraints in printing resolutions have hindered the implementation of 3D printed chromatography columns into industrial settings and practical applications in the separation science. Over the past decade, researchers have started working on adapting existing printable materials or creating novel ones specifically for chromatography applications. This review seeks to delve deeper into the materials used for 3D printing adsorptive stationary phases and their implication for both 3D printing and separations. By exploring the advancements, challenges, and future potentials, we aim to shed the light on how this manufacturing approach can reshape the landscape of chromatography stationary phases offering new possibilities for improved separation performance and new applications.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"192 ","pages":"Article 118301"},"PeriodicalIF":11.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}