Trends in Analytical Chemistry最新文献

{"title":"From selection to sensing: recent advances in aptamer-based fluorescent and colorimetric platforms for illicit drug monitoring","authors":"Yuyang Li ,&nbsp;Yingnan Wei ,&nbsp;Jinyi Zhang ,&nbsp;Yao Lin ,&nbsp;Chengbin Zheng","doi":"10.1016/j.trac.2025.118457","DOIUrl":"10.1016/j.trac.2025.118457","url":null,"abstract":"<div><div>Driven by the proliferation of opioids and new psychoactive substances, the global crisis of illicit drug abuse underscores an urgent need for rapid and portable detection tools for law enforcement and public health interventions. Recent advancements in aptamer-based fluorescent and colorimetric sensors offer a transformative alternative, combining their high sensitivity, specificity, and adaptability for on-site applications. This review critically highlights the aptamer selection advances for illicit drug small molecules, including breakthroughs in high-affinity molecular recognition and addressing screening challenges. Furthermore, the emerging optical sensing platforms and their innovations in signal transduction mechanisms and actual sample applications were highlighted. We also discuss emerging trends and future directions in aptamer-based optical sensors for illicit drug detection. The significance of this review lies in advancing the development of rapid and reliable sensing technologies for illicit drug detection, providing potential tools for roadside drug screening, border security, and forensic investigations.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118457"},"PeriodicalIF":12.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-imprinted materials towards artificial antibodies: Technique, application and perspective","authors":"Lukuan Liu ,&nbsp;Jianhui Liu ,&nbsp;Weijie Zhang ,&nbsp;Zhen Liang ,&nbsp;Yukui Zhang ,&nbsp;Jing Liu ,&nbsp;Kaiguang Yang ,&nbsp;Lihua Zhang","doi":"10.1016/j.trac.2025.118453","DOIUrl":"10.1016/j.trac.2025.118453","url":null,"abstract":"<div><div>Bio-imprinted materials (BIMs) are artificial antibodies that use biological macromolecules as templates to create molecularly imprinted materials with precise recognition capabilities. They exhibit good physicochemical robustness under extreme conditions, making them ideal for complex biological samples. This review summarizes the fundamental principles of BIMs and the bio-imprinting technology, with a focus on their applications in various biomedical fields, including post-translational modification analysis, virus detection, extracellular vesicles and cell analysis, spatial imaging and localization, drug delivery systems, and antibody-like therapeutics. This review also discusses key technical challenges such as non-specific protein adsorption, poor batch-to-batch reproducibility, and insufficient stability in physiological conditions. Furthermore, this review provides perspectives on the use of artificial intelligence-assisted molecular design to optimize imprinting efficiency, integration strategies with existing biotechnologies, and potential future application, offering new technological pathways for the advancement of life sciences and medicine.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118453"},"PeriodicalIF":12.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatilomics in diseases odour and electronic nose diagnosis","authors":"Danyao Qu ,&nbsp;Taoping Liu ,&nbsp;Yueyi Cheng ,&nbsp;Tao Du ,&nbsp;Bolang Cheng ,&nbsp;Yong Zhang ,&nbsp;Chen Su ,&nbsp;Youbing Zheng ,&nbsp;Xi Xu ,&nbsp;Gang Wang ,&nbsp;Xin Hu ,&nbsp;Ruizhi Ning ,&nbsp;Miaomiao Yuan ,&nbsp;Weiwei Wu","doi":"10.1016/j.trac.2025.118440","DOIUrl":"10.1016/j.trac.2025.118440","url":null,"abstract":"<div><div>The innate human aspiration for health preservation and effective disease prevention represents a fundamental driver of civilizational progress. The large-scale screening and early diagnosis are valuable that the medical cost can be greatly reduced while prognostic trajectories are optimized. In this review article, the concepts, advances, and critical thinking on volatile markers of specific diseases (Volatilomics), electronic nose technology, and its applications in diagnosis through volatiles, are systematically introduced. This article provides valuable guidance for researchers in this interdisciplinary field, offering insights into state-of-the-art detection technologies for medical professionals while also assisting chemists, materials scientists, and electronics engineers in selecting target VOCs for sensor development.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118440"},"PeriodicalIF":12.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing food integrity: Harnessing the power of ensemble learning algorithms","authors":"Samaneh Ehsani ,&nbsp;Philipp Weller ,&nbsp;Hadi Parastar","doi":"10.1016/j.trac.2025.118458","DOIUrl":"10.1016/j.trac.2025.118458","url":null,"abstract":"<div><div>The term “ensemble learning” refers to a class of techniques in machine learning (ML) that blends manifold individual models, so-called base learners to improve the performance and enhance the robustness of the overall model. In the context of food integrity, where authenticity, safety and quality are crucial aspects, ensemble learning methods have become popular over the years. Due to the vast applicability of ensemble learning algorithms in food analysis, the objective of this review is to introduce the principles of ensemble learning to a broad audience in a simplified way without complex mathematical details and formulas. Moreover, typical applications of ensemble learning algorithms in various fields of food analyses are covered. As ensemble methods feature high predictive power, they are useful for building robust models in food integrity problems. Techniques based on ensemble learning have usually better prediction results in terms of accuracy, sensitivity, and specificity than classical ML methods.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118458"},"PeriodicalIF":12.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances and applications in integrating nucleic acid amplification technologies and CRISPR systems by spatial / temporal separation strategies for one-pot diagnosis","authors":"Di Huang ,&nbsp;Yichen He ,&nbsp;Chutian Xu ,&nbsp;Peijie Shen ,&nbsp;Shanshan Shi ,&nbsp;Qiang Shu ,&nbsp;Xiangming Fang ,&nbsp;Zhinan Xu","doi":"10.1016/j.trac.2025.118456","DOIUrl":"10.1016/j.trac.2025.118456","url":null,"abstract":"<div><div>The integration of nucleic acid amplification techniques (NAATs) with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems has enabled a new generation of molecular diagnosis that combines high sensitivity with single-nucleotide specificity. More importantly, consolidating both processes into a unified and airtight “one-pot” format without “non-closed” reagent transfer steps holds substantial promise for point-of-care testing (POCT) in resource-limited settings, particularly in clinical situations requiring rapid responses, such as during pandemics. This approach offers streamlined workflows, faster turnaround times, and reduced risk of contamination. However, the intrinsic biochemical incompatibility between amplification enzymes and CRISPR nucleases, as the former typically generate more nucleic acids while the latter consumes them to produce a signal, particularly due to competing reaction kinetics, poses a major challenge for one-pot assay development. In recent years, significant progress has been made in addressing this issue through a range of biochemical and engineering strategies. This review systematically categorizes these strategies based on whether NAATs and CRISPR systems are fully separated at the onset of the reaction, classifying them into spatial separation and temporal separation approaches. We highlight key representative studies within each category and evaluate their underlying mechanisms, performance trade-offs, and practical limitations. Furthermore, we discuss the remaining challenges that hinder clinical translation and provide insights into future directions, such as integration with upstream sample preparation and downstream signal readout modules. We hope this review not only provides a coherent roadmap for the rational design of one-pot platforms but also inspires broader innovation at the interface of molecular engineering in real-world healthcare applications.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118456"},"PeriodicalIF":12.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advancements in dual modality biosensors for detecting disease causing pathogens","authors":"Sutanu Sarkar ,&nbsp;Uddipan Dasgupta ,&nbsp;Malabika Ghosh ,&nbsp;Akhilesh Babu Ganganboina ,&nbsp;Ankan Dutta Chowdhury","doi":"10.1016/j.trac.2025.118454","DOIUrl":"10.1016/j.trac.2025.118454","url":null,"abstract":"<div><div>Early detection of infectious diseases is crucial for timely therapeutic intervention. Biosensors have emerged as vital tools for analyte detection across biomedical, food safety, and clinical diagnostics. Despite their promising advancement, conventional biosensors face challenges such as signal noise and environmental variability. Additionally, issues like cross-reactivity and calibration difficulties in miniaturized devices compromise their accuracy and reliability. To address these limitations, dual modal biosensors have been developed, integrating two complementary detection techniques to enhance applicability. By cross-validating data, these biosensors can significantly reduce false positive and negative results while providing reliable measurements in complex biological matrices. Furthermore, dual modal biosensors expand the range of analytes and improve detection limits. This review explores the fundamental principles of various biosensing modalities and highlights their collaborative integration as a promising disease diagnostics approach. Translation challenges are also discussed, and future directions are proposed to bridge the gap between laboratory research and real-world applications.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118454"},"PeriodicalIF":12.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combatting organophosphate toxicity: Novel strategies for detection, analysis, and sensor development for public health protection","authors":"Ajay Gupta ,&nbsp;Kanchan Sharma ,&nbsp;Gaurav Pandey ,&nbsp;Shiv Dutta Lawaniya ,&nbsp;Harsh Pandey ,&nbsp;Kumud Kant Awasthi ,&nbsp;Kamlendra Awasthi ,&nbsp;Anjali Awasthi","doi":"10.1016/j.trac.2025.118451","DOIUrl":"10.1016/j.trac.2025.118451","url":null,"abstract":"<div><div>The extensive use of organophosphate pesticides (OPs) worldwide poses a serious health risk due to their accumulation in humans and animals. Detecting these hazardous insecticides in environmental and agricultural samples is crucial for assessing their residual concentrations against permissible limits set by regulatory agencies across different nations. Some organophosphates, exhibit higher toxicity, necessitating advancements in modern detection techniques with improved sensitivity, expanded detection ranges, and enhanced resistance to interference. Traditional methods, including chromatographic and spectroscopic techniques, offer high accuracy but are often costly, time-intensive, and require skilled operators. This review aims to provide a comprehensive overview of pesticides for last ten years, including their types, uses, and toxicity. It explores the current state of organophosphate insecticide detection while discussing various detection methods, emphasizing the limitations of conventional techniques and the potential of emerging nanomaterial-based sensing approaches with a special focus on electrochemical sensing and its integration with microfluidics, and portable sensing platforms highlighting recent trends and advancements.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118451"},"PeriodicalIF":12.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of isothermal amplification methods in DNA hydrogels for biosensing","authors":"Kawthar Abdallah ,&nbsp;Morteza Hosseini ,&nbsp;Yasaman-Sadat Borghei ,&nbsp;Jiuxing Li ,&nbsp;Bijan Ranjbar","doi":"10.1016/j.trac.2025.118452","DOIUrl":"10.1016/j.trac.2025.118452","url":null,"abstract":"<div><div>DNA hydrogels provide molecular-level programmable recognition and excellent biocompatibility for biosensing but are plagued by serious drawbacks like mechanical instability, reduced sensitivity, expensive fabrication processes, and poor single-target detection. These intrinsic limitations have driven the search for new approaches that can concurrently boost and maintain the advantageous properties of hydrogel-based systems. Isothermal nucleic acid amplification techniques (INAATs) have transformed molecular diagnostics by eliminating thermal cycling requirements through enzyme-dependent strategies (LAMP, RPA, RCA, SDA, and EXPAR) and enzyme-independent strategies (HCR, CHA, and EDC) with high sensitivity and rapid amplification capabilities. However, INAATs typically depend on complex instrumentation for signal readout and interpretation. The strategic coupling of INAATs with DNA hydrogels overcomes the limitations of both approaches by synergistic means. This hybrid platform enhances hydrogel structural properties, reduces equipment dependency, extends detection capabilities, and uses INAAT amplification properties to improve biosensing performance through increased sensitivity. This review describes INAAT integration within DNA hydrogels for high-performance biosensing applications. We introduce the most recent diverse applications in this newly developed area and elucidate the ensuing combination mechanisms and effects of integrating each INAAT strategy into hydrogel systems, comparing their standalone performances and competencies. We demonstrate how the integration of cutting-edge biotools in these platforms, particularly CRISPR-based detection platforms and catalytic/enzymatic amplification methods, enables higher specificity and signal amplification functionalities. Finally, we identify the key challenges and set the top development priorities to propel clinical translation.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118452"},"PeriodicalIF":12.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart hydrogel sensors: advancing from highly sensitive detection to an integrated platform for miRNA diagnosis and treatment","authors":"Xiaoyu Zhang,&nbsp;Yuanke Zhang,&nbsp;Wenjing Wei,&nbsp;Guixia Ling,&nbsp;Peng Zhang","doi":"10.1016/j.trac.2025.118450","DOIUrl":"10.1016/j.trac.2025.118450","url":null,"abstract":"<div><div>Abnormal expression of microRNAs (miRNAs), as disease-specific biomarkers, contains crucial pathological information. Their accurate detection facilitates early intervention, vital for warning and diagnosing major diseases like cancer. This review focuses on innovative applications of multifunctional hydrogel sensors in miRNA detection. It systematically analyzes hydrogel materials’ functional design strategies, highlights progress in signal amplification mechanisms, and wearable integration technologies. Meanwhile, it explores the potential of this technology in the integration of diagnosis and treatment, and proposes solutions to the current bottlenecks and challenges of detection technologies in combination with actual scenario needs. Finally, it envisions integrating multimodal sensing with precision medical closed loops to build an intelligent miRNA detection platform. This offers theoretical support and practical methods for developing highly sensitive, portable systems, significantly promoting innovation in clinical early diagnosis technologies.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118450"},"PeriodicalIF":12.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence, analytical methods, and ecotoxicological effects of 6PPD-Quinone in aquatic environments: A review","authors":"Bingyi Wang,&nbsp;Wenhui Sun,&nbsp;Xindi Ye,&nbsp;Zhiquan Liu,&nbsp;Hangjun Zhang","doi":"10.1016/j.trac.2025.118449","DOIUrl":"10.1016/j.trac.2025.118449","url":null,"abstract":"<div><div>The release of 6PPD-quinone (6PPD-Q), a transformation product of the tire antioxidant N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD), has attracted considerable research attention. However, knowledge of 6PPD-Q fate and effects in the aquatic environment remain limited. Here, we systematically review 6PPD-Q detection techniques in aquatic environments, current environmental pollution status, and 6PPD-Q’s toxic effects toward aquatic organisms. Currently, 6PPD-Q is mainly detected using high-performance liquid chromatography-tandem mass spectrometry and ultra-high-performance liquid chromatography-tandem high-resolution mass spectrometry. 6PPD-Q is widely detected in road runoff, snowmelt, and river waters with concentrations ranging from 4 × 10⁻⁵ to 19 μg/L, which exceed the acute toxicity thresholds for sensitive species, such as coho salmo. In addition, the potential adverse biological effects included developmental toxicity, neurotoxicity, and oxidative stress. Ultimately, these effects may contribute to ecological risk in contaminated aquatic systems. Finally, future directions were highlight for the detection methods, environmental behaviors, toxicity mechanisms, and long-term ecological effects of 6PPD-Q.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"193 ","pages":"Article 118449"},"PeriodicalIF":12.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}