Exploring the potential of oligonucleotide mapping with liquid chromatography – mass spectrometry to study the primary structure of mRNA

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-05-16 DOI:10.1016/j.trac.2025.118309

Jonathan Maurer , Helena Vanluchene , Athanasios Tsalmpouris , Kris Morreel , Julien Camperi , Koen Sandra , Davy Guillarme

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引用次数: 0

Abstract

Oligonucleotide (ON) or RNA mapping using liquid chromatography coupled to mass spectrometry (LC-MS) has recently emerged as an attractive approach to support the development of nucleic acid-based medicines, in particular mRNA. This technique provides valuable information on the primary structure of mRNA by combining enzymatic digestion, high-resolution chromatographic separation, accurate mass determination, optimized fragmentation strategies and advanced data treatment, similar to the peptide mapping workflows commonly employed for structural elucidation and quality assessment of therapeutic proteins.

This review critically examines the latest developments in enzymatic digestion methods, liquid chromatography techniques, mass spectrometry conditions, and data treatment strategies to improve the performance of ON mapping and also provides some perspectives to further optimize this workflow.

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利用液相色谱-质谱法研究mRNA初级结构的寡核苷酸定位

利用液相色谱-质谱联用（LC-MS）进行寡核苷酸（ON）或RNA定位，最近成为一种有吸引力的方法，以支持基于核酸的药物的开发，特别是mRNA。该技术通过结合酶切、高分辨率色谱分离、精确的质量测定、优化的片段策略和先进的数据处理，提供了mRNA初级结构的宝贵信息，类似于通常用于结构阐明和治疗蛋白质量评估的肽图谱工作流程。本文综述了酶消化方法、液相色谱技术、质谱条件和数据处理策略的最新发展，以提高ON制图的性能，并提供了进一步优化该工作流程的一些观点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.