Byulleten Sibirskoy Meditsiny最新文献

{"title":"The level of metabolic hormones in young people with arterial hypertension against the background of abdominal obesity","authors":"Ya. V. Polonskaya, E. V. Kashtanova, E. M. Stakhneva, V. S. Shramko, E. V. Sadovski, S. R. Ledovskikh, L. V. Shcherbakova, E. V. Garbuzova, A. D. Khudyakova, Yu. I. Ragino","doi":"10.20538/1682-0363-2023-3-74-79","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-74-79","url":null,"abstract":"Aim. To study the level of metabolic hormones in young people with arterial hypertension (AH) against the background of abdominal obesity (AO). Materials and methods. The study included 498 people who were divided into two groups. The experimental group encompassed 250 people with AH, of which – 159 people had AO, the average systolic pressure – 141.9 ± 13.9 mm Hg, diastolic pressure – 95.6 ± 7.5 mm Hg. The control group included 248 people comparable to the experimental group by gender and age, of whom 104 people had AO, the average systolic pressure – 118.5 ± 9.8 mm Hg, diastolic pressure – 77.8 ± 7.4 mm Hg. The levels of amylin, C-peptide, ghrelin, glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide-1 (GLP-1), glucagon, insulin, pancreatic polypeptide (PP), and peptide YY (PYY) were determined by the multiplex analysis. The level of glucose was determined by the enzymatic method. Statistical processing of the results was carried out using the SPSS 13.0 software. Results. Patients with AH had higher levels of amylin, C-peptide, and glucose and lower levels of PYY. There was no significant difference between the experimental group and the control group for the rest of the studied parameters. In the experimental group, the C-peptide, GLP-1, glucagon, and insulin levels were associated with AO. In the control group, the association of AO with the levels of C-peptide, insulin, and glucose was shown. The odds of AH in people under the age of 45 years were associated with a decrease in the level of PYY, a rise in the amylin levels, and an increase in waist circumference. Conclusion. Of the studied metabolic hormones, an increased level of amylin and reduced PYY can serve as potential biomarkers indicating high odds of developing AH in people under 45 years of age. AO is a factor that contributes to the development of AH at a young age.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"All you need to know about sarcopenia: a short guide for an internal medicine physician in questions and answers","authors":"G. R. Bikbavova, M. A. Livzan, D. V. Tikhonravova","doi":"10.20538/1682-0363-2023-3-88-97","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-88-97","url":null,"abstract":"Sarcopenia is associated with social, economic, and individual burdens, including loss of independence, poor quality of life, and disability. In a short period of time, ideas about sarcopenia transformed from geriatric syndrome to disease. Initially, sarcopenia was considered in the context of gradual age-related deterioration in the functioning of all physiological systems. Over the years, it became clear that it can develop a second time, as a consequence of various diseases and pathological conditions. To date, there have been no generally accepted diagnostic criteria for sarcopenia. There are several tests and tools available for screening sarcopenia, the choice of which depends on physical capabilities of the patient, capabilities of the medical institution, and the purpose for which it is detected (research or clinical practice). From the point of view of human health, sarcopenia increases the risk of falls and fractures; impairs the ability to perform daily activities; is associated with the progression of major diseases and cognitive impairments; leads to movement disorders; contributes to a decrease in the quality of life, loss of independence or a need for long-term care. The presence of sarcopenia increases both the risk of hospitalization and hospitalization costs. The aim of the literature review is to provide an analysis of up-to-date information on the causes, pathogenesis, screening, diagnosis, treatment, and consequences of sarcopenia, myosteatosis, and sarcopenic obesity. The search for literature containing information on relevant studies was conducted in PubMed and Google Scholar by the following keywords: sarcopenia, dynapenia, myosteatosis, sarcopenic obesity, nutritional status, malnutrition.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Βeta-adrenergic receptor reactivity of erythrocyte membranes in patients with left or right atrial dilatation against the background of atrial fibrillation","authors":"E. F. Muslimova, V. O. Popova, T. Y. Rebrova, E. A. Archakov, R. E. Batalov, S. A. Afanasiev","doi":"10.20538/1682-0363-2023-3-61-67","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-61-67","url":null,"abstract":"Hyperactivation of the sympathoadrenal system (SAS) leads to desensitization of β1-adrenergic receptors (β1-AR). This contributes to aggravation of myocardial contractile dysfunction and development of arrhythmias, including atrial fibrillation (AF). An indirect indicator of the viability of β1-AR is β-adrenergic receptor reactivity of erythrocyte membranes (β-ARM). Aim. To evaluate β-ARM in patients with different forms of AF, including left (LAD) or right (RAD) atrial dilation. Materials and methods. The sample included 38 patients, 65.8% of whom had paroxysmal AF, 21% had persistent AF, and 13.2% had long-standing persistent AF. All patients received surgical treatment for AF by radiofrequency ablation or cryoablation. LAD was detected in 39.4% of patients, RAD – in 34.2% of patients. Βeta-ARM was determined before treatment, as well as at 3 days and at 12 months after ablation. Results. The groups of patients with different forms of AF, as well as patients with LAD / RAD and without it showed comparable values of β-ARM at different measurement periods. In the group of patients without LAD / RAD, β-ARM increased 3 days after ablation compared to β-ARM before the treatment (p = 0.002 / p = 0.004) and returned to the pre-treatment level after 3 months. At the same time, in the group of patients with LAD / RAD, β-ARM did not significantly change before the ablation and in different periods after it. Conclusion. In patients with AF without LAD / RAD, we detected an increase in β-ARM 3 days after the ablation compared to the level before the treatment and a decrease in the intensity of SAS 3 months after the surgery. In the presence of LAD / RAD, no changes in the β-ARM were revealed.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of <i>MnSOD</i> and <i>GPX1</i> gene polymorphisms with a risk of chronic dust-induced bronchitis","authors":"A. G. Zhukova, A. S. Kazitskaya, T. K. Yadykina, O. N. Gulyaeva","doi":"10.20538/1682-0363-2023-3-36-42","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-36-42","url":null,"abstract":"Aim. To assess the association of the MnSOD (rs4880) and GPX1 (rs1050450) gene polymorphisms with a risk of developing chronic dust-induced bronchitis in workers of the coal mining industry. Materials and methods. The study included 182 coal miners with prolonged exposure to high concentrations of coal dust, including 116 people with a previously established diagnosis of chronic dust-induced bronchitis (CDB) and 66 people without pathology of the bronchopulmonary system, working under the same sanitary and hygienic conditions. Polymorphisms of the MnSOD (rs4880) and GPX1 (rs1050450) genes were studied using polymerase chain reaction. Results. For the first time, we established a statistically significant association between the polymorphisms of the MnSOD (rs4880) and GPX1 genes (rs1050450) and CDB. Thus, the chance of detecting the homozygous A/A (Val/Val) MnSOD genotype in miners with CDB was 2 times higher than in the comparison group ( χ 2 – 5.42; р = 0.02; odds ratio (OR) 2.21; 95% confidence interval (CI) 1.13–4.33), while the chance of detecting the homozygous G/G (Pro/Pro) GPX1 genotype in miners with CDB was almost 6 times higher than in the comparison group (χ2 – 21.47; р = 0.001; OR 5.89; 95% CI 2.65–13.08). It was found that the combination of AA/GG genotypes of the MnSOD / GPX1 genes was significantly associated with a 1.5-fold risk of developing CDB (χ 2 – 11.49; р ˂ 0.001; relative risk (RR) 1.59; 95% CI 1.36–1.84), while the chance of detecting this combination of genotypes in miners with bronchopulmonary pathology was 15 times higher than in the comparison group (OR 15.09; 95% CI 1.99–114.64). Conclusion. Carriage of homozygous genotypes A/A at the rs4880 MnSOD locus and G/G at the rs1050450 GPX1 locus was shown to be a marker of genetic predisposition to the development of CDB. The combination of homozygous genotypes of the studied AA/GG MnSOD / GPX1 genes indicated a 1.5-fold risk of developing CDB. Carrying one of the three combinations of the MnSOD and GPX1 genotypes (GG/AA, AA/AA, and AG/AA) indicated resistance to the development of CDB.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of generalized anxiety disorder symptoms and their associations with behavioral attitudes and perception of the future in the Russian youth","authors":"A. G. Peshkovskaya, S. A. Galkin, A. V. Larionova, A. N. Kornetov","doi":"10.20538/1682-0363-2023-3-68-73","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-68-73","url":null,"abstract":"Aim. To investigate the prevalence of generalized anxiety disorder symptoms and their associations with behavioral attitudes and perception of the future among the Russian youth. Materials and methods. The study involved 1,300 people aged 16–25 years and was conducted online in October 2022 using online questionnaires, which included the GAD-7 screening questionnaire on the severity of anxiety symptoms. Results. According to the questionnaire, 25.5% of young people who participated in the study reported medium to high intensity of anxiety symptoms. These respondents were significantly more likely to perceive the country’ future negatively (p = 0.002). In addition, the largest proportion of people who reported a motive to leave the country (38.6%) was registered among young people who were at risk of generalized anxiety disorder. The authors emphasize that the study results should be interpreted in relation to the time period of data collection. Conclusion. The study showed that generalized anxiety disorder symptoms negatively affected the perception of the future among young people and highlighted the need for primary psychological prevention in this age group.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a hydrogel derived from porcine dermis for experimental treatment of superficial wounds","authors":"K. I. Melkonyan, Ya. A. Kozmay, T. V. Rusinova, G. P. Chuprynin, M. I. Kartashevskaya, I. I. Kartashevsky, S. V. Storozhuk, I. I. Selezneva, K. G. Gurevich","doi":"10.20538/1682-0363-2023-3-54-60","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-54-60","url":null,"abstract":"Aim. To study the efficacy of dermal hydrogel application in the experimental treatment of superficial scarified wounds in rats. Materials and methods. The hydrogel was obtained from porcine dermis by alkaline hydrolysis. The DNA concentration was determined using the Nano Drop ND-1000 spectrophotometer. The study included 30 male Sphinx rats. Scarified wounds were created on the rat skin, then the rats were divided into two groups: group 1 – rats without treatment, or control group (n = 15), group 2 – rats with wound treatment with the dermal hydrogel for 5 days, or experimental group (n = 15). On day 3, 7, and 14 of the experiment, we explanted skin samples from the wound area and performed routine H&E staining. Results. On day 3 of the experiment, moderate inflammation, edema, and collagen fiber disorganization were revealed in the experimental group, and pronounced inflammation with purulent exudate was found in the control group. On day 7 of the experiment, inflammation and foci of stratified epithelium were detected in the control group. The histologic analysis of the skin samples from the experimental group showed pronounced plethora of the vessels, necrotic changes of the dermis, and edema. The total thickness of the epidermis and the thickness of its stratum corneum were greater than in the control group samples. On day 14, the differences between the groups were minimal and the epidermis was thickened in the experimental group animals. Conclusion. The study examined the effects of the dermal hydrogel on scarified wounds in rats. We found faster skin regeneration (by 1.5–2 days) in the experimental group compared to the controls. Besides, the rats of the experimental group were characterized by an increase in the number of fibroblasts in the dermis and thickened epidermis in the affected area.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of humoral markers in patients with anthracycline-related cardiac dysfunction","authors":"E. V. Grakova, K. V. Kopeva, S. N. Shilov, E. T. Bobyleva, E. N. Berezikova, V. V. Kalyuzhin, A. T. Teplyakov","doi":"10.20538/1682-0363-2023-3-25-35","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-25-35","url":null,"abstract":"Aim. To carry out a 12-month study on the prognostic role of humoral markers responsible for the main mechanisms of initiation of cardiotoxic myocardial damage (endothelin-1, soluble Fas-L, N-terminal pro-brain natriuretic peptide (NT-proBNP), tumor necrosis factor-α, interleukin (IL)-1β, matrix metalloproteinase (MMP)-2 and MMP9, soluble form of the ST2 protein (sST2), a tissue inhibitor of metalloproteinase-1, and tetranectin) in assessing the risk of progression of anthracycline-related left ventricular dysfunction. Materials and methods. The study included a total of 114 women aged 48.0 (46.0; 52.0) years without concomitant cardiovascular diseases and risk factors who received chemotherapy with anthracyclines in the past. The levels of serum biomarkers were determined using the enzyme immunoassay. Transthoracic echocardiography was performed at baseline and at 12 months of follow-up. Results. After 12 months of follow-up, all patients were retrospectively divided into 2 groups: group 1 (n = 54) included patients with an unfavorable course of anthracycline-related cardiac dysfunction (ARCD), group 2 (n = 60) encompassed patients with a favorable course of the disease. According to the ROC analysis, MMP-2 ≥ ≥ 338.8 pg / ml (sensitivity 57%, specificity 78%; AUC = 0.629; p = 0.025), MMP-9 ≥ 22.18 pg / ml (sensitivity 89%, specificity 87%; AUC = 0.886; p < 0.001), sST2 ≥ 32.4 ng / ml (sensitivity 64%, specificity 70.5%; AUC = 0.691; p = 0.002), and tetranectin ≤ 15.4 pg / ml (sensitivity 69%, specificity 72%; AUC = 0.764; p < 0.001) were identified as predictors of an adverse course of ARCD. When comparing ROC curves, it was found that the concentration of MMP-9 (p = 0.002) was the most significant predictor of the progression of ARCD. Conclusion. MMP-2 and -9, soluble ST2, and tetranectin can be considered as non-invasive markers for assessing the risk of ARCD progression. At the same time, an increased level of MMP-9 is the most significant predictor of ARCD progression.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136038502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and prognostic value of leptin resistance in the hospital period of myocardial infarction","authors":"E. E. Gorbatovskaya, Ya. A. Dyleva, E. V. Belik, E. G. Uchasova, R. S. Tarasov, V. V. Kashtalap, O. V. Gruzdeva","doi":"10.20538/1682-0363-2023-3-14-24","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-14-24","url":null,"abstract":"Aim. To evaluate the prevalence of leptin resistance (LR) and its clinical and prognostic value in association with metabolic disorders and features of the proinflammatory state in the hospital period of myocardial infarction. Materials and methods. The study included 114 men diagnosed with ST segment elevation myocardial infarction (MI). On day 1 and 12 of MI, the levels of leptin and leptin receptor were measured in patients, and the free leptin index (FLI) was calculated. Leptin resistance (LR) was recorded at leptin &gt; 6.45 ng / ml and FLI &gt; 25. A comparative analysis of clinical and anamnestic characteristics, biochemical parameters, and cardiovascular prognosis was carried out between patients with and without LR. Statistical data processing was carried out using the Statistica 10.0 software package and SPSS 17.0 for Windows . Results. The prevalence of LR in the hospital period of MI was 64%. LR was associated with cardiovascular pathology in the family history, arterial hypertension, dyslipidemia, and obesity. The presence of LR was accompanied by a significant increase in the level of glucose, free fatty acids (FFA), and interleukin (IL)-6 on day 1 of MI and by a significant rise in insulin, C-peptide, tumor necrosis factor (TNF)-alpha, and plasminogen activator inhibitor-1 (PAI-1) throughout the hospital stay. Patients with LR were characterized by multi-vessel and more severe lesions of the coronary bed and were more often subject to early post-infarction angina, recurrent MI, rhythm and conduction disturbances during hospital stay for MI. Conclusion. Patients with MI are characterized by high prevalence of LR during the hospital stay. LR is associated with cardiovascular risk factors, metabolic disorders, formation of insulin resistance, and increased proinflammatory and prothrombogenic factors. The identified features in the presence of LR probably contribute to the development of adverse cardiovascular events in the hospital period of MI.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136182381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A direct comparison of the diagnostic efficacy of alternative scaffoldbased radiopharmaceuticals [&lt;sup&gt;99m&lt;/sup&gt;Tc]Tc-ADAPT6 and [&lt;sup&gt;99m&lt;/sup&gt;Tc]Tc-(HE)&lt;sub&gt;3&lt;/sub&gt;-G3 in patients with HER2-positive breast cancer","authors":"O. D. Bragina, S. M. Deyev, E. Yu. Garbukov, V. E. Goldberg, V. I. Chernov, V. M. Tolmachev","doi":"10.20538/1682-0363-2023-3-6-13","DOIUrl":"https://doi.org/10.20538/1682-0363-2023-3-6-13","url":null,"abstract":"Aim. To perform a direct comparison of the diagnostic efficacy of [ 99m Tc]Tc-ADAPT6 and [ 99m Tc]Tc-(HE) -G3 in HER2-positive breast cancer patients before the systemic treatment. Materials and methods. The study included 11 patients with HER2-positive breast cancer (T1–4N0–2M0–1) before the initiation of systemic treatment. All patients underwent a radionuclide examination with [ 99m Tc]TcADAPT6 and [ 99m Tc]Tc-(HE) -G3 with the interval of 3–4 days. Single-photon emission computed tomography (SPECT) /computed tomography (CT) was performed 2 and 4 hours after [ 99m Tc]Tc-ADAPT6 and [ 99m Tc]Tc(HE) 3 -G3 administration, respectively. Results. The analysis of [ 99m Tc]Tc-ADAPT6 and [ 99m Tc]Tc-(HE) -G3 distribution showed their high uptake in the kidneys and liver. Breast tumors were visualized in all cases. The average tumor uptake of [99mTc]Tc-ADAPT6 was 4.7 ± 2.1, which was significantly higher than in the [99mTc]Tc-(HE)3-G3 injection (3.5 ± 1.7) (p < 0.005, paired t-test). The tumor-to-background ratio (15.2 ± 7.4 and 19.6 ± 12.4, respectively) had no statistical differences in both cases (p > 0.05, paired t-test). Liver metastases were visualized in patients 1 and 5 and corresponded to the projection of metastases according to contrast-enhanced abdominal CT. The accumulation of [ 99m Tc]Tc-ADAPT6 and [ 99m Tc]Tc-(HE)3-G3 in the projection of metastases in both cases was significantly higher compared to the primary tumor (1.3 and 1.7 times higher in patient 1; 2.2 and 3.5 times higher in patient 5, respectively). Conclusion. Both [ 99m Tc]Tc-ADAPT6 and [ 99m Tc]Tc-(HE)3-G3 demonstrated the diagnostic efficacy in visualizing a primary HER2-positive tumor in breast cancer patients. However, [99mTc]Tc-ADEPT6 had higher accumulation values, which makes it a more promising diagnostic agent.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136182380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Local biocompatibility and biochemical profile of hepatic cytolysis in subcutaneous implantation of polylactide matrices","authors":"E. A. Ivanova, A. N. Dzyuman, M. V. Dvornichenko","doi":"10.20538/1682-0363-2022-4-63-71","DOIUrl":"https://doi.org/10.20538/1682-0363-2022-4-63-71","url":null,"abstract":"The aim of the study was to investigate local biocompatibility and systemic effects of nonwoven polylactide (PLA) matrices on blood and liver parameters after their subcutaneous implantation in Wistar rats. Materials and methods. Bioabsorbable fibrous PLA matrices were produced by electrospinning and had dimensions (10 × 10 mm², thickness of no more than 0.5 mm; fiber diameter in the matrix ~1 μm) appropriate for subcutaneous implantation in white laboratory rats. Polymer implants were sterilized in ethylene oxide vapor. Thirty days after the implantation of PLA matrices, local biocompatibility according to GOST ISO 10993-6-2011, cellular parameters (total leukocyte count, hemogram, erythrocyte count, hemoglobin concentration), and biochemical blood parameters (lactate concentration, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels) were studied, and a standard histologic evaluation of the liver was performed. Results. PLA matrix samples were mild local irritants on a scale of 1–1.9 points according to GOST ISO 10993-6-2011 criteria 30 days after the subcutaneous implantation. The median density of distribution of multinucleated giant cells (MNGCs) in the connective tissue around and in PLA matrices was 1,500 (1,350; 1,550) per 1 mm² of a slice. Pronounced leukocytic reaction due to lymphocytosis was noted (an increase by 1.7 times compared with a sham-operated (SO) control group, р < 0.02). The absence of a significant neutrophil count in the blood revealed sterile inflammation proceeding in the subcutaneous tissue around the PLA materials. Normalization of hepatic cytolysis markers (ALT and AST activity) in the blood without pronounced changes in the structure of the liver and the number of binuclear hepatocytes was noted. These markers were increased in SO controls (ALT up to 123% and AST up to 142%, p < 0.001 compared with values in the intact group). Conclusion. Nonwoven PLA matrices are biocompatible with subcutaneous tissue, undergo bioresorption by MNGCs, and have a distant protective effect on the functional state of the liver in laboratory animals. Hypotheses on the detected systemic effect during subcutaneous implantation of PLA matrices were discussed; however, specific mechanisms require further study.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134916430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}