猪真皮水凝胶在浅表伤口实验性治疗中的应用

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
K. I. Melkonyan, Ya. A. Kozmay, T. V. Rusinova, G. P. Chuprynin, M. I. Kartashevskaya, I. I. Kartashevsky, S. V. Storozhuk, I. I. Selezneva, K. G. Gurevich
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引用次数: 0

摘要

的目标。目的:研究真皮水凝胶应用于实验性大鼠浅表瘢痕创面的治疗效果。材料和方法。以猪真皮层为原料,采用碱性水解法制备水凝胶。采用纳米滴ND-1000分光光度计测定DNA浓度。这项研究包括30只雄性狮身人面像大鼠。在大鼠皮肤上创面,将大鼠分为两组:1组(未处理)或对照组(n = 15), 2组(皮肤水凝胶处理5 d)或实验组(n = 15)。在实验的第3、7、14天,我们从创面处取出皮肤样本,进行常规H&E染色。结果。实验第3天,实验组出现中度炎症、水肿、胶原纤维紊乱,对照组出现明显炎症并有化脓性渗出。在实验第7天,对照组检测到炎症和分层上皮灶。实验组皮肤样本的组织学分析显示明显的血管过多,真皮坏死改变和水肿。表皮总厚度和角质层厚度均大于对照组。第14天,各组间差异极小,试验组动物表皮增厚。结论。本研究考察了皮肤水凝胶对大鼠疤痕伤口的影响。我们发现实验组的皮肤再生速度比对照组快(1.5-2天)。此外,实验组大鼠真皮成纤维细胞数量增加,患处表皮增厚。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Application of a hydrogel derived from porcine dermis for experimental treatment of superficial wounds
Aim. To study the efficacy of dermal hydrogel application in the experimental treatment of superficial scarified wounds in rats. Materials and methods. The hydrogel was obtained from porcine dermis by alkaline hydrolysis. The DNA concentration was determined using the Nano Drop ND-1000 spectrophotometer. The study included 30 male Sphinx rats. Scarified wounds were created on the rat skin, then the rats were divided into two groups: group 1 – rats without treatment, or control group (n = 15), group 2 – rats with wound treatment with the dermal hydrogel for 5 days, or experimental group (n = 15). On day 3, 7, and 14 of the experiment, we explanted skin samples from the wound area and performed routine H&E staining. Results. On day 3 of the experiment, moderate inflammation, edema, and collagen fiber disorganization were revealed in the experimental group, and pronounced inflammation with purulent exudate was found in the control group. On day 7 of the experiment, inflammation and foci of stratified epithelium were detected in the control group. The histologic analysis of the skin samples from the experimental group showed pronounced plethora of the vessels, necrotic changes of the dermis, and edema. The total thickness of the epidermis and the thickness of its stratum corneum were greater than in the control group samples. On day 14, the differences between the groups were minimal and the epidermis was thickened in the experimental group animals. Conclusion. The study examined the effects of the dermal hydrogel on scarified wounds in rats. We found faster skin regeneration (by 1.5–2 days) in the experimental group compared to the controls. Besides, the rats of the experimental group were characterized by an increase in the number of fibroblasts in the dermis and thickened epidermis in the affected area.
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来源期刊
Byulleten Sibirskoy Meditsiny
Byulleten Sibirskoy Meditsiny MEDICINE, GENERAL & INTERNAL-
CiteScore
0.70
自引率
50.00%
发文量
102
审稿时长
8 weeks
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