Byulleten Sibirskoy Meditsiny最新文献

{"title":"Dissimilar populations of EpСam-positive cells in ascitic fluid of ovarian cancer patients: a relationship with the degree of carcinomatosis","authors":"E. Kaigorodova, M. O. Ochirov, S. Molchanov, R. R. Rogachev, D. Dyakov, A. Chernyshova, O. Shpileva, O. I. Kovalev, S. Vtorushin","doi":"10.20538/1682-0363-2021-2-44-53","DOIUrl":"https://doi.org/10.20538/1682-0363-2021-2-44-53","url":null,"abstract":"Background . Peritoneal carcinomatosis (PC) is one of the most unfavorable factors of ovarian cancer progression. It is often accompanied by accumulation of fluid in the abdominal cavity, which is called ascites. However, prognostic factors associated with malignant ascites are not well understood. The aim of this study was to evaluate dissimilar populations of EpCAM-positive cells in ascitic fluid and their relationship with the presence of invasive peritoneal implants and the prevalence of carcinomatosis on the Predictive Index Value (PIV) scale in ovarian cancer patients. Materials and methods . The prospective study included 22 patients aged 36–76 years with newly diagnosed FIGO stage Ic–IV ovarian cancer, who were admitted for treatment to Cancer Research Institute of Tomsk NRMC. The study material included EDTA-stabilized ascitic fluid sampled during laparoscopy. Various populations of ascites tumor cells were identified by laser multicolor flow cytometry. The degree of carcinomatosis was determined according to the PIV scale. Results. The study identified twelve populations of EpCAM-positive cells in the ascitic fluid of ovarian cancer patients. Epcam+CD45-CD44-CD24+CD133-Ncadherin+ cells ( r = 0.58; p = 0.004) and atypical (hybrid) EpCam+CD45+CD44+CD24+/-CD133+/-Ncadherin+/- cells ( r = = 0.51; р = 0.01) had a positive correlation with the PIV index. Conclusion . The obtained results show a high degree of heterogeneity of tumor cells in the ascitic fluid of ovarian cancer patients. Identified atypical (hybrid) forms of EpCam-positive cells are of great interest for cell biology and require further investigation.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2021-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Цитокины и атеросклероз – новые направления исследований","authors":"С. В. Дутова, Ю. В. Саранчина, М. Р. Карпова, О. Ю. Килина, Н. Г. Польша, Т. С. Кулакова, Н. В. Ханарин","doi":"10.20538/1682-0363-2018-4-199-207","DOIUrl":"https://doi.org/10.20538/1682-0363-2018-4-199-207","url":null,"abstract":"This review generalizes the current evidence on the content of the pro- and anti-inflammatory cytokines in patients’ blood serum with atherosclerotic lesions of the coronary, carotid and iliac arteries. The results gave the ability to assess cytokine immune cells of atherosclerotic plaques. Also the results of national and international research allowed the assessment of the prognostic value of cytokine content.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Моделирование функциональных кист яичников путем введения фолликулостимулирующего гормона","authors":"Daria A. Trus, O. A. Tikhonovskaya, A. Okorokov, Liliya R. Mustafina, O. S. Timofeeva, S. V. Logvinov, A. V. Gerasimov, Elena Yu. Varakuta, Aleksey V. Potapov, G. A. Suhanova, Irina G. Kutsenko","doi":"10.20538/1682-0363-2017-3-137-144","DOIUrl":"https://doi.org/10.20538/1682-0363-2017-3-137-144","url":null,"abstract":"Aim. To study the morphofunctional state of ovaries after the introduction of recombinant FSH. Materials and methods. Two groups of mature female Wistar rats were used in the study. The experimental group consisted of 35 rats with a model of follicular ovarian cysts, while 25 rats were in the control group. Rats were taken out of the experiment on days 3, 7, 15, 30 and 60. Paraffin slides of ovaries were stained with hematoxylin and eosin by Van Gieson. Histological and morphometric investigations were performed. Results. On day 7 of FSH administration, the maximum increase in the size of the ovaries was observed due to the formation of single-cell follicular cysts. Rats in the experimental group showed a marked decrease in the number of growing follicles on day 7 and 15. The increase in atretic bodies and follicles in comparison with the control group was observed on day 7 and lasted until the end of the experiment. Additionally, on day 7 of the experiment, hyperemia and vasoconstriction were noted. The number of yellow bodies decreased during the experiment, and an increase in collagen formation occurred starting from day 15 of the experiment. Conclusion. The introduction of follicle-stimulating hormone for 7 days leads to disruption of folliculogenesis, strengthening of atresia in the ovaries, and the formation of functional cysts.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Пластический бронхит, ассоциированный с корригированным пороком сердца у ребенка","authors":"E. M. Kamaltynova, E. V. Krivoshchekov, Olga S. Yanulevich, Elena S. Kavardakova","doi":"10.20538/1682-0363-2017-2-180-186","DOIUrl":"https://doi.org/10.20538/1682-0363-2017-2-180-186","url":null,"abstract":"Plastic bronchitis (PB) is a rare disease that causes airway obstruction with large «rubber» bronchial casts in adults and children, mainly due to concomitant cardiac or pulmonary pathology. It involves the organization of exudate or transudate, which takes the form of the respiratory tract. Mortality with PB reaches 50%. Exact epidemiological data are not known. In most patients, PB occurs in the context of major diseases, which can be conditionally divided into two groups: heart and vascular disease and secondary complication of pulmonary diseases. Usually PB manifests with episodes of dyspnea, tachycardia, hypoxia, fever and cough associated with airway obstruction. Sometimes the symptoms resemble asthma or obstructive bronchitis, accompanied by wheezing and the symptoms of respiratory failure, decreasing of saturation (of what?), the development of pneumothorax. All existing methods of treatment are symptomatic. Promising methods of therapy are the use of inhaled unfractionated heparin and a recombinant tissue plasminogen activator (TAP). The article presents a clinical case of PB in a girl of 5 years with the background of corrected cardiac anomaly.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49320824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Неинвазивная вентиляция легких у больных ХОБЛ, госпитализированных в стационар","authors":"S. Avdeev","doi":"10.20538/1682-0363-2017-2-6-19","DOIUrl":"https://doi.org/10.20538/1682-0363-2017-2-6-19","url":null,"abstract":"The use of noninvasive ventilation (NIV) of lungs during the last two decades significantly improved the results of management of severe COPD patients with acute or chronic respiratory failure during both periods of exacerbation and stability. At present, NIV is considered to be the first-line therapy for acute exacerbation of COPD with hypercapnia and respiratory acidosis. This method of airway support turned out to be effective in patients after extubation both for the relief of excommunication from a respirator and for prophylaxis and treatment of postextubation respiratory failure. NIV was proven to be successful in patients with a combination of COPD and obstructive sleep apnea (overlap syndrome), in COPD with pneumonia and in postoperative COPD patients who have undergone lung resectional surgery. The efficacy of NIV under intensive care and intensive therapy unit conditions has stimulated the interest to the use of mask ventilation in hospitals and out-patient departments (for a prolonged domestic therapy). This article presents a review of NIV use in patients with COPD during both periods of exacerbation and stability.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47590182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Исследование реакции системы гемокоагуляции на тканевую гипоксию у пациентов с хронической обструктивной болезнью легких","authors":"Anna A. Bulanova, Artem E. Aksenenko, Anna S. Bobrovnikova, Galina V. Dudko, Dmitriy S. Slizevich, E. Bukreeva, M. N. Shpisman, Ivan Tyutrin","doi":"10.20538/1682-0363-2017-2-87-95","DOIUrl":"https://doi.org/10.20538/1682-0363-2017-2-87-95","url":null,"abstract":"Background. Nowadays little data related to the hemostatic system and fibrinolysis in patients with chronic obstructive pulmonary disease (COPD) are available. This is due to the lack of standardized methods for studying the hemostasis system, as well as to the lack of a single functional test that allows the evaluation of the complete fibrinogenesis cycle in whole blood. Aim. The aim of our study was to develop a functional test capable of analyzing the blood gas composition in the “point-of-care test” method for the evaluation of the hemostatic potential in patients with COPD, based on a standardized test stimulus, which is tissue hypoxia. The current level of clinical and laboratory diagnostics requires personification and research of the hemo-coagulation system in real time (point-of-care test), which allows low-frequency piezotromboelastography(NVTEG) to be performed. Materials and methods. NVTEG was chosen to estimate the state of the hemocoagulation system. Ten patients with COPD and 10 healthy volunteers were examined. Hypoxia was selected as a standardized test stimulus. Hypoxia conditions were caused by smoking one standard cigarette (composition: resin 10 mg/cig., nicotine 0,7 mg/cig., CO 10 mg/cig.). The degree of tissue hypoxia was assessed with the GASTAT-navi blood gas analyzer. Results. The study has shown that in response to the standard test stimulus, which is the tissue hypoxia caused by smoking of a standardized cigarette, two types of haemostatic potential reaction were detected both in patients with COPD and healthy volunteers. The first type of reaction – “hypercoagulation” – is characterized by the formation of chronometric and structural hypercoagulation at all stages of fibrinogenesis and increased coagulation activity by 25–30% compared with the response in healthy individuals. The second type of reaction – “hypocoagulation” – is characterized by the formation of chronometric and structural hypocoagulation, a decrease in coagulation activity by 25–30% compared with the response in healthy individuals. Conclusion. Test stimulus, which acts as tissue hypoxia, causes a uniform spectrum of changes in the blood gas composition and hemocoagulation system in both healthy volunteers and patients with COPD. The possibility of online assessment of all stages of fibrinogenesis makes it possible to stratify patients with COPD by type of reaction, which is certain to have an important diagnostic and prognostic value and in the future will allow a more personified approach for choosing the treatment tactics.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of grapes polyphenol concentrate “Fenokor” in terms of hypoxic myocardial injury","authors":"I. Zadnipryany, O. S. Tretiakova, A. Kubyshkin, T. Sataieva","doi":"10.20538/1682-0363-2017-3-34-42","DOIUrl":"https://doi.org/10.20538/1682-0363-2017-3-34-42","url":null,"abstract":"","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Глютеновая энтеропатия: клинические наблюдения","authors":"Yu. A. Emel’yanova, G. R. Bikbavova, T. V. Tretyakova, A. V. Pavlov, O. V. Martunenko","doi":"10.20538/1682-0363-2016-4-150-155","DOIUrl":"https://doi.org/10.20538/1682-0363-2016-4-150-155","url":null,"abstract":"Presented clinical cases of patients with a diagnosis of gluten enteropathy in treatment in the department of gastroenterology Regional Clinical Hospital. The case is of interest to doctors of different specialties for the differential diagnosis of anemia and malabsorption syndrome, demonstrate both the classic version, and atypical forms of the disease course. Diagnosis of celiac disease is based on three key positions: clinical findings, histology and serological markers. The clinical picture of celiac disease is characterized by pronounced polymorphism, by going beyond the a gastroenterological pathology. For screening of gluten sensitive celiac typically used an antibody to tissue transglutaminase. Morphological research of the mucous membrane of the small intestine is the determining criterion in the diagnosis of celiac disease. The use of specific gluten-free diet leads to the positive dynamics of the disease and improve the quality of life of patients.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2016-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Informed Consent in a Decision-making on Participation in The Clinical Trial: Multicenter study in Russia \"Face to Face\"","authors":"O. Zvonareva, E. S. Kulikov, I. Deev, O. Kobyakova, O. S. Fedorova, N. Kirillova, E. A. Starovoytova, N. Zhukova, I. Zhukova, V. V. Esip, E. D. Schastnyy, N. V. Khurkhurova, L. Nikitina, T. Golosova, I. Pimenov, M. A. Netaeva, S. Fedosenko","doi":"10.20538/1682-0363-2016-4-40-51","DOIUrl":"https://doi.org/10.20538/1682-0363-2016-4-40-51","url":null,"abstract":"Introduction . Currently, clinical trials (CT) remain the only technology, which provides proof of efficacy and safety of new drugs and their subsequent release to the market. Medical researcher and informed consent (IC) are the main (and often the only) source of information for the patient about the upcoming clinical trials, and thus have a direct impact on the perception of clinical trials, and on the patient’s decision about participation. However, the degree of influence of these factors on the clinical trials participants still remains unclear. Materials and methods. A multicenter cross-sectional study was conducted in different cities of the Russian Federation. Patients who had previous experience in CTs (or were enrolled in a CT at the time of this study) were asked to complete a questionnaire. Results. To assess the impact of researcher, all respondents were divided into 2 groups: patients that acquainted with IC in collaboration with the researcher, and the other group, which reviewed IC form independently. We evaluated the importance of the factors influencing the decision-making process on participation in clinical trials. According to our data, the most important factors were professional monitoring services (3,72 ± 1,00), regular condition monitoring (3,66 ± 0,98), and better medical care (3,62 ± 1,00). These factors were evaluated at significantly lower score by group of patients that acquainted with IC together with the researcher (3,55 ±0,94, vs 4,01 ± 0,90, p = 0,002; 3,52 ± 1,01 vs 3,87 ± 0,90, p = 0,040; 3,49 ± 0,94, vs 3,83 ± 1,06, p = 0,020 respectively). In assessing the factors that had negative impact on the interest in participating in a clinical trial, the most significant were risk of side effects (3,01 ± 1,27), study of new medication (2,68 ± 1,21), and the risk of getting into the placebo group (2,64 ± 1,34) (so-called “objective” risk factors). At the same time, risk of side effects and risk of getting into the placebo group were also assessed at significantly lower score by  group of patients that acquainted with IC together with the researcher (2,87 ± 1,28, vs 3,33 ± 1,17, p = 0,024; 2,51 ±1,25, vs 3,03 ± 1,34, p = 0,022 respectively). Furthermore, it was found that in the case of the researcher’s assistance acquaintance time with IC reduced threefold. We also evaluated the effect of the complexity of IC text on the decision-making process on participation in clinical trials. The group of respondents, who rated the IC as easy, appeared to be more interested in the final results of the study. Conclusion. Thus, when assessing the impact of the researcher on the review process of informed consent with the decision to participate in clinical trials, we found that in the case of assistance of the researcher, the acquaintance time with IC is reduced three times. In addition, this group of patients during the conversation with the researcher shows better and more clear understanding of the nature and general methodology ","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2016-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Создание и устойчивое развитие специализированных центров как способ повышения качества медицинской помощи","authors":"V. I. Guzeva, O. V. Guzeva, V. V. Guzeva, D. O. Dukhanina, V. Kasumov","doi":"10.20538/1682-0363-2016-4-20-29","DOIUrl":"https://doi.org/10.20538/1682-0363-2016-4-20-29","url":null,"abstract":"Quality of care is evaluated on the completeness of the survey, the correct diagnosis, treatment efficacy, and its duration. Improving the quality and efficiency of medical care for children with paroxysmal disorders of consciousness is one of topical problems of neurology. Aim. The aim of the work is to justify the relationship between improving the quality of health care and sustainable development in the modern conditions of specialized medical centers on the example of the work on the identification and treatment of children with paroxysmal disorders of consciousness of the Center for diagnosis and treatment of epilepsy, and sleep disorders in children and adolescents at the department neurology, neurosurgery and medical genetics SPbGPMU. Materials and methods. For more accurate diagnosis and treatment at the Center conducted a comprehensive examination, including video-EEG оf 527 children aged 1 month to 18 years. A clinical trial study included medical cases, assessment of neurological and somatic status, the study of seizure types and forms of the disease. Instrumental methods of examination were determined by EEG and MRI studies of the brain. Main results. Comprehensive survey of sick children with monitoring video-EEG revealed that 317 children (60,1%) had epileptic paroxysms and 210 children (39,8%) – non-epileptic paroxysms. Correction treatment was performed in 284 (89,5%) children with epileptic paroxysms and altered the treatment in 190 (90,4%) children with epileptic paroxysms. Conclusion. The presented clinical data show the high effectiveness of the Centre in the diagnosis and treatment of children with paroxysmal disorders of consciousness. The accumulated experience in the Center confirms the relevance of the creation of the structure of scientific and educational institutions specialized centers in which patients will be given to high-quality medical care.","PeriodicalId":43691,"journal":{"name":"Byulleten Sibirskoy Meditsiny","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2016-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67686309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}