Journal of Xenobiotics最新文献_第10页

Novel Technique for Simultaneous Ethylene Glycol and Its Metabolites Determination in Human Whole Blood and Urine Samples Using GC-QqQ-MS/MS. 利用 GC-QqQ-MS/MS 同时测定人体全血和尿液样本中乙二醇及其代谢物的新技术

IF 6.8

Journal of Xenobiotics Pub Date : 2024-08-27 DOI: 10.3390/jox14030065

Kaja Tusiewicz, Olga Wachełko, Marcin Zawadzki, Paweł Szpot

{"title":"Novel Technique for Simultaneous Ethylene Glycol and Its Metabolites Determination in Human Whole Blood and Urine Samples Using GC-QqQ-MS/MS.","authors":"Kaja Tusiewicz, Olga Wachełko, Marcin Zawadzki, Paweł Szpot","doi":"10.3390/jox14030065","DOIUrl":"10.3390/jox14030065","url":null,"abstract":"Toxicological analyses often necessitate the identification of compounds belonging to diverse functional groups. For GC-MS analyses, derivatization of compounds belonging to different functional groups can pose a challenge and requires the development of comprehensive methods of analysis. One example could be ethylene glycol, whose widespread use is related to possible unintentional or suicidal intoxications. This fact clearly indicates the need to develop sensitive methods for the determination of ethylene glycol and its metabolites in biological material, as only such complex analysis allows for proper toxicological expertise. A simultaneous GC-QqQ-MS/MS method for the determination of ethylene glycol together with its metabolites, glyoxal and glycolic acid, as well as the detection of glyoxylic acid and oxalic acid, was developed and fully validated. A novel approach for simultaneous derivatization of substances from different groups (alcohols, aldehydes, and carboxylic acids) was established. Sample preparation included the addition of three internal standards (BHB-d4, ethylene glycol-d4 and methylglyoxal), precipitation with acetonitrile and subsequent derivatization with N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA), as well as pentafluorophenylhydrazine (PFPH). Detection was carried out with the use of triple quadrupole mass spectrometer. The ionization method was electron impact, and quantitative analysis was carried out in multiple reaction monitoring mode. The lower limit of quantification was 1 μg/mL, 0.1 μg/mL, and 500 μg/mL for ethylene glycol, glyoxal, and glycolic acid, respectively. The presented method was applied in three authentic postmortem cases of ethylene glycol intoxication.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1143-1164"},"PeriodicalIF":6.8,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142297972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vanadium Toxicity Is Altered by Global Warming Conditions in Sea Urchin Embryos: Metal Bioaccumulation, Cell Stress Response and Apoptosis. 全球变暖条件改变了海胆胚胎中钒的毒性：金属生物累积、细胞应激反应和细胞凋亡。

IF 6.8

Journal of Xenobiotics Pub Date : 2024-08-22 DOI: 10.3390/jox14030064

Chiara Martino, Fabiana Geraci, Rosaria Scudiero, Giampaolo Barone, Flores Naselli, Roberto Chiarelli

{"title":"Vanadium Toxicity Is Altered by Global Warming Conditions in Sea Urchin Embryos: Metal Bioaccumulation, Cell Stress Response and Apoptosis.","authors":"Chiara Martino, Fabiana Geraci, Rosaria Scudiero, Giampaolo Barone, Flores Naselli, Roberto Chiarelli","doi":"10.3390/jox14030064","DOIUrl":"10.3390/jox14030064","url":null,"abstract":"In recent decades, the global vanadium (V) industry has been steadily growing, together with interest in the potential use of V compounds as therapeutics, leading to V release in the marine environment and making it an emerging pollutant. Since climate change can amplify the sensitivity of marine organisms already facing chemical contamination in coastal areas, here, for the first time, we investigated the combined impact of V and global warming conditions on the development of Paracentrotus lividus sea urchin embryos. Embryo-larval bioassays were carried out in embryos exposed for 24 and 48 h to sodium orthovanadate (Na3VO4) under conditions of near-future ocean warming projections (+3 °C, 21 °C) and of extreme warming at present-day marine heatwave conditions (+6 °C, 24 °C), compared to the control temperature (18 °C). We found that the concomitant exposure to V and higher temperature caused an increased percentage of malformations, impaired skeleton growth, the induction of heat shock protein (HSP)-mediated cell stress response and the activation of apoptosis. We also found a time- and temperature-dependent increase in V bioaccumulation, with a concomitant reduction in intracellular calcium ions (Ca2+). This work demonstrates that embryos' sensitivity to V pollution is increased under global warming conditions, highlighting the need for studies on multiple stressors.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1130-1142"},"PeriodicalIF":6.8,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142297977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Therapeutic Mechanisms of Honey in Mitigating Toxicity from Anticancer Chemotherapy Toxicity: A Review. 蜂蜜减轻抗癌化疗毒性的治疗机制：综述。

IF 6.8

Journal of Xenobiotics Pub Date : 2024-08-20 DOI: 10.3390/jox14030063

Debalina Bose, Ademola C Famurewa, Aman Akash, Eman M Othman

{"title":"The Therapeutic Mechanisms of Honey in Mitigating Toxicity from Anticancer Chemotherapy Toxicity: A Review.","authors":"Debalina Bose, Ademola C Famurewa, Aman Akash, Eman M Othman","doi":"10.3390/jox14030063","DOIUrl":"10.3390/jox14030063","url":null,"abstract":"Within the domain of conventional oncochemotherapeutics, anticancer chemotherapy (AC) has emerged as a potent strategy for the treatment of cancers. AC is the mainstay strategy for solid and non-solid cancer treatment. Its mechanistic action targets the blockage of DNA transcription and the dysregulation of cell cycle machinery in cancer cells, leading to the activation of death pathways. However, the attendant side effect of toxicity inflicted by AC on healthy tissues presents a formidable challenge. The crucial culprit in the AC side effect of toxicity is unknown, although oxidative stress, mitochondrial impairment, inflammatory cascades, autophagy dysregulation, apoptosis, and certain aberrant signaling have been implicated. Honey is a natural bee product with significant health benefits and pharmacological properties. Interestingly, the literature reports that honey may proffer a protection mechanism for delicate tissue/organs against the side effect of toxicity from AC. Thus, this review delves into the prospective role of honey as an alleviator of the AC side effect of toxicity; it provides an elucidation of the mechanisms of AC toxicity and honey's molecular mechanisms of mitigation. The review endeavors to unravel the specific molecular cascades by which honey orchestrates its mitigating effects, with the overarching objective of refining its application as an adjuvant natural product. Honey supplementation prevents AC toxicity via the inhibition of oxidative stress, NF-κB-mediated inflammation, and caspase-dependent apoptosis cascades. Although there is a need for increased mechanistic studies, honey is a natural product that could mitigate the various toxicities induced by AC.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1109-1129"},"PeriodicalIF":6.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Berberine Attenuates Acetamiprid Exposure-Induced Mitochondrial Dysfunction and Apoptosis in Rats via Regulating the Antioxidant Defense System. 小檗碱通过调节抗氧化防御系统减轻啶虫脒暴露诱导的大鼠线粒体功能障碍和细胞凋亡

IF 6.8

Journal of Xenobiotics Pub Date : 2024-08-07 DOI: 10.3390/jox14030061

Annu Phogat, Jagjeet Singh, Reena Sheoran, Arun Hasanpuri, Aakash Chaudhary, Shakti Bhardwaj, Sandeep Antil, Vijay Kumar, Chandra Prakash, Vinay Malik

{"title":"Berberine Attenuates Acetamiprid Exposure-Induced Mitochondrial Dysfunction and Apoptosis in Rats via Regulating the Antioxidant Defense System.","authors":"Annu Phogat, Jagjeet Singh, Reena Sheoran, Arun Hasanpuri, Aakash Chaudhary, Shakti Bhardwaj, Sandeep Antil, Vijay Kumar, Chandra Prakash, Vinay Malik","doi":"10.3390/jox14030061","DOIUrl":"10.3390/jox14030061","url":null,"abstract":"Acetamiprid (ACMP) is a neonicotinoid insecticide that poses a significant threat to the environment and mankind. Oxidative stress and mitochondrial dysfunction are considered prime contributors to ACMP-induced toxic effects. Meanwhile, berberine (BBR) a natural plant alkaloid, is a topic of interest because of its therapeutic and prophylactic actions. Therefore, this study evaluated the effects of BBR on ACMP-mediated alterations in mitochondrial functions and apoptosis in rat liver tissue. Male Wistar rats were divided into four groups: (I) control, (II) BBR-treated, (III) ACMP-exposed, and (IV) BBR+ACMP co-treated groups. The doses of BBR (150 mg/kg b.wt) and ACMP (1/10 of LD50, i.e., 21.7 mg/kg b.wt) were given intragastrically for 21 consecutive days. The results showed that the administration of ACMP diminished mitochondrial complex activity, downregulated complex I (ND1 and ND2) and complex IV (COX1 and COX4) subunit mRNA expression, depleted the antioxidant defense system, and induced apoptosis in rat liver. BBR pre-treatment significantly attenuated ACMP-induced mitochondrial dysfunction by maintaining mitochondrial complex activity and upregulating ND1, ND2, COX1, and COX4 mRNA expression. BBR reversed ACMP-mediated apoptosis by diminishing Bax and caspase-3 and increasing the Bcl-2 protein level. BBR also improved the mitochondrial antioxidant defense system by upregulating mRNA expression of PGC-1α, MnSOD, and UCP-2 in rat liver tissue. This study is the first to evaluate the protective potential of BBR against pesticide-induced mitochondrial dysfunction in liver tissue. In conclusion, BBR offers protection against ACMP-induced impairment in mitochondrial functions by maintaining the antioxidant level and modulating the apoptotic cascade.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1079-1092"},"PeriodicalIF":6.8,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estrogenic Responsiveness of Brown Trout Primary Hepatocyte Spheroids to Environmental Levels of 17α-Ethinylestradiol. 褐鳟原代肝细胞球体对环境水平 17α 乙炔基雌二醇的雌激素反应性

IF 6.8

Journal of Xenobiotics Pub Date : 2024-08-06 DOI: 10.3390/jox14030060

Rodrigo F Alves, Célia Lopes, Eduardo Rocha, Tânia Vieira Madureira

{"title":"Estrogenic Responsiveness of Brown Trout Primary Hepatocyte Spheroids to Environmental Levels of 17α-Ethinylestradiol.","authors":"Rodrigo F Alves, Célia Lopes, Eduardo Rocha, Tânia Vieira Madureira","doi":"10.3390/jox14030060","DOIUrl":"10.3390/jox14030060","url":null,"abstract":"Three-dimensional (3D) fish hepatocyte cultures are promising alternative models for replicating in vivo data. Few studies have attempted to characterise the structure and function of fish 3D liver models and illustrate their applicability. This study aimed to further characterise a previously established spheroid model obtained from juvenile brown trout (Salmo trutta) primary hepatocytes under estrogenic stimulation. The spheroids were exposed for six days to environmentally relevant concentrations of 17α-ethinylestradiol-EE2 (1-100 ng/L). The mRNA levels of peroxisome (catalase-Cat and urate oxidase-Uox), lipid metabolism (acyl-CoA long chain synthetase 1-Acsl1, apolipoprotein AI-ApoAI, and fatty acid binding protein 1-Fabp1), and estrogen-related (estrogen receptor α-ERα, estrogen receptor β-ERβ, vitellogenin A-VtgA, zona pellucida glycoprotein 2.5-ZP2.5, and zona pellucida glycoprotein 3a.2-ZP3a.2) target genes were evaluated by quantitative real-time polymerase chain reaction. Immunohistochemistry was used to assess Vtg and ZP protein expressions. At the highest EE2 concentration, VtgA and ZP2.5 genes were significantly upregulated. The remaining target genes were not significantly altered by EE2. Vtg and ZP immunostaining was consistently increased in spheroids exposed to 50 and 100 ng/L of EE2, whereas lower EE2 levels resulted in a weaker signal. EE2 did not induce significant changes in the spheroids' viability and morphological parameters. This study identified EE2 effects at environmentally relevant doses in trout liver spheroids, indicating its usefulness as a proxy for in vivo impacts of xenoestrogens.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1064-1078"},"PeriodicalIF":6.8,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prenatal Exposure to Bisphenol A and/or Diethylhexyl Phthalate Impacts Brain Monoamine Levels in Rat Offspring. 产前接触双酚 A 和/或邻苯二甲酸二乙基己酯会影响大鼠后代的脑单胺水平。

IF 6.8

Journal of Xenobiotics Pub Date : 2024-08-01 DOI: 10.3390/jox14030058

Amrita Kaimal, Jessica M Hooversmith, Maryam H Al Mansi, Philip V Holmes, Puliyur S MohanKumar, Sheba M J MohanKumar

{"title":"Prenatal Exposure to Bisphenol A and/or Diethylhexyl Phthalate Impacts Brain Monoamine Levels in Rat Offspring.","authors":"Amrita Kaimal, Jessica M Hooversmith, Maryam H Al Mansi, Philip V Holmes, Puliyur S MohanKumar, Sheba M J MohanKumar","doi":"10.3390/jox14030058","DOIUrl":"10.3390/jox14030058","url":null,"abstract":"This study examines the sex-specific effects of gestational exposure (days 6-21) to endocrine-disrupting chemicals such as bisphenol A (BPA), diethylhexyl phthalate (DEHP), or their combination on brain monoamine levels that play an important role in regulating behavior. Pregnant Sprague-Dawley rats were orally administered saline, low doses (5 µg/kg BW/day) of BPA or DEHP, and their combination or a high dose (7.5 mg/kg BW/day) of DEHP alone or in combination with BPA during pregnancy. The offspring were subjected to a behavioral test and sacrificed in adulthood, and the brains were analyzed for neurotransmitter levels. In the paraventricular nucleus, there was a marked reduction in dopamine levels (p < 0.01) in male offspring from the BPA, DEHP, and B + D (HD) groups, which correlated well with their shock probe defensive burying times. Neurotransmitter changes in all brain regions examined were significant in female offspring, with DEHP (HD) females being affected the most, followed by the B + D groups. BPA and/or DEHP (LD) increased monoamine turnover in a region-specific manner in male offspring (p < 0.05). Overall, prenatal exposure to BPA, DEHP, or their combination alters monoamine levels in a brain region-specific, sex-specific, and dose-dependent manner, which could have implications for their behavioral and neuroendocrine effects.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1036-1050"},"PeriodicalIF":6.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between Liver and Kidney Function and Birth Outcomes in Pregnant Surinamese Women Exposed to Mercury and Lead in the Caribbean Consortium for Research in Environmental and Occupational Health (CCREOH) Environmental Epidemiologic Cohort Study. 加勒比环境与职业健康研究联合会（CCREOH）环境流行病学队列研究中暴露于汞和铅的苏里南孕妇的肝肾功能与出生结果之间的关系。

IF 6.8

Journal of Xenobiotics Pub Date : 2024-08-01 DOI: 10.3390/jox14030059

Sheila Kort, Jeffrey Wickliffe, Arti Shankar, Hannah H Covert, Maureen Lichtveld, Wilco Zijlmans

{"title":"Association between Liver and Kidney Function and Birth Outcomes in Pregnant Surinamese Women Exposed to Mercury and Lead in the Caribbean Consortium for Research in Environmental and Occupational Health (CCREOH) Environmental Epidemiologic Cohort Study.","authors":"Sheila Kort, Jeffrey Wickliffe, Arti Shankar, Hannah H Covert, Maureen Lichtveld, Wilco Zijlmans","doi":"10.3390/jox14030059","DOIUrl":"10.3390/jox14030059","url":null,"abstract":"Exposure to mercury (Hg) and lead (Pb), in combination with liver and kidney impairment, may result in adverse birth outcomes. From 408 women in the age range of 16 to 46 years, living in rural and urban areas in the interior of Suriname, we looked at the association between adverse birth outcomes and exposure to Hg and Pb in combination with liver and kidney function. This group of women represented a subcohort of pregnant women who participated in the Caribbean Consortium for Research in Environmental and Occupational Health (CCREOH)-Meki Tamara study. Liver function was assessed by measuring aspartate amino transferase (AST), alanine amino transferase (ALT), and gamma-glutamyl transferase (GGT). Kidney function was assessed by measuring creatinine, urea, and cystatin C. We defined preterm births as birth before 37 weeks of gestation, low birthweight as birthweight < 2500 g, and low Apgar score as a score < 7 at 5 min, and these were used as indicators for adverse birth outcomes. Small size for gestational age was defined as gestational age < -2SD weight for GA. We found significant statistical associations between biomarkers for liver and kidney functions and adverse birth outcomes Apgar score and gestational age. No significant association was found between heavy metals Hg and lead and adverse birth outcomes.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1051-1063"},"PeriodicalIF":6.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incorporating Tissue-Specific Gene Expression Data to Improve Chemical-Disease Inference of in Silico Toxicogenomics Methods. 纳入组织特异性基因表达数据，改进硅学毒物基因组学方法的化学-疾病推断。

IF 6.8

Journal of Xenobiotics Pub Date : 2024-07-31 DOI: 10.3390/jox14030057

Shan-Shan Wang, Chia-Chi Wang, Chien-Lun Wang, Ying-Chi Lin, Chun-Wei Tung

{"title":"Incorporating Tissue-Specific Gene Expression Data to Improve Chemical-Disease Inference of in Silico Toxicogenomics Methods.","authors":"Shan-Shan Wang, Chia-Chi Wang, Chien-Lun Wang, Ying-Chi Lin, Chun-Wei Tung","doi":"10.3390/jox14030057","DOIUrl":"10.3390/jox14030057","url":null,"abstract":"In silico toxicogenomics methods are resource- and time-efficient approaches for inferring chemical-protein-disease associations with potential mechanism information for exploring toxicological effects. However, current in silico toxicogenomics systems make inferences based on only chemical-protein interactions without considering tissue-specific gene/protein expressions. As a result, inferred diseases could be overpredicted with false positives. In this work, six tissue-specific expression datasets of genes and proteins were collected from the Expression Atlas. Genes were then categorized into high, medium, and low expression levels in a tissue- and dataset-specific manner. Subsequently, the tissue-specific expression datasets were incorporated into the chemical-protein-disease inference process of our ChemDIS system by filtering out relatively low-expressed genes. By incorporating tissue-specific gene/protein expression data, the enrichment rate for chemical-disease inference was largely improved with up to 62.26% improvement. A case study of melamine showed the ability of the proposed method to identify more specific disease terms that are consistent with the literature. A user-friendly user interface was implemented in the ChemDIS system. The methodology is expected to be useful for chemical-disease inference and can be implemented for other in silico toxicogenomics tools.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1023-1035"},"PeriodicalIF":6.8,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vivo Antischistosomicidal and Immunomodulatory Effects of Dietary Supplementation with Taraxacum officinale. 蒲公英膳食补充剂的体内抗肉毒桿菌杀灭作用和免疫调节作用

IF 6.8

Journal of Xenobiotics Pub Date : 2024-07-29 DOI: 10.3390/jox14030056

Amany Ebrahim Nofal, Amal Mohamed Shaaban, Hany Mohammed Ibrahim, Faten Abouelmagd, Azza Hassan Mohamed

{"title":"In Vivo Antischistosomicidal and Immunomodulatory Effects of Dietary Supplementation with Taraxacum officinale.","authors":"Amany Ebrahim Nofal, Amal Mohamed Shaaban, Hany Mohammed Ibrahim, Faten Abouelmagd, Azza Hassan Mohamed","doi":"10.3390/jox14030056","DOIUrl":"10.3390/jox14030056","url":null,"abstract":"Bilharziasis is a widespread trematode parasite that poses a severe public health burden. Dandelion (Taraxacum officinale) has several pharmacological and traditional properties critical for treating several hepatic disorders. The present study was designed to assess the potential efficacy of T. officinale root (TOR) dietary supplementation with or without praziquantel (PZQ) against liver and intestinal disorders in mice infected with Schistosoma mansoni. This study was conducted on five groups; G1: uninfected control, G2: untreated S. mansoni-infected mice, G3: infected animals treated with 250 mg/kg PZQ for three alternative days, G4: infected animals were orally administered 600 mg/kg bw TOR daily for 10 days, and G5: infected animals that received both PZQ and TOR as previously described. The current findings after different treatments indicated topographical scanning electron microscopy alterations of male adult worms and a critical reduction in worm burden, ova count, granuloma diameter, hepatic and intestinal histological abnormalities, fibrosis, immunohistochemical expression of CD3+ and CD20+ cells, oxidative stress, and interleukin-10, also upregulation of interferon-gamma, and antioxidant enzymes, when compared to the infected untreated mice. The best results were obtained in mice administered PZQ+TOR together because of their antioxidant properties and ability to promote the host immune response to parasitic infection.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"1003-1022"},"PeriodicalIF":6.8,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142074150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic Exposure to Both Electronic and Conventional Cigarettes Alters Ileum and Colon Turnover, Immune Function, and Barrier Integrity in Mice. 长期接触电子香烟和传统香烟会改变小鼠回肠和结肠的翻转、免疫功能和屏障完整性。

IF 6.8

Journal of Xenobiotics Pub Date : 2024-07-22 DOI: 10.3390/jox14030053

Madjid Djouina, Anaïs Ollivier, Christophe Waxin, Gwenola Kervoaze, Muriel Pichavant, Ségolène Caboche, Djamal Achour, Céline Grare, Delphine Beury, David Hot, Sébastien Anthérieu, Jean-Marc Lo-Guidice, Laurent Dubuquoy, David Launay, Cécile Vignal, Philippe Gosset, Mathilde Body-Malapel

{"title":"Chronic Exposure to Both Electronic and Conventional Cigarettes Alters Ileum and Colon Turnover, Immune Function, and Barrier Integrity in Mice.","authors":"Madjid Djouina, Anaïs Ollivier, Christophe Waxin, Gwenola Kervoaze, Muriel Pichavant, Ségolène Caboche, Djamal Achour, Céline Grare, Delphine Beury, David Hot, Sébastien Anthérieu, Jean-Marc Lo-Guidice, Laurent Dubuquoy, David Launay, Cécile Vignal, Philippe Gosset, Mathilde Body-Malapel","doi":"10.3390/jox14030053","DOIUrl":"10.3390/jox14030053","url":null,"abstract":"Although the effects of cigarette smoke (CS) on the development of several intestinal diseases is well documented, the impact of e-cigarette aerosol (e-cig) on digestive health is largely unknown. To compare the effects of e-cig and CS on mouse ileum and colon, animals were chronically exposed for 6 months by nose-only inhalation to e-cig at 18 or 30 W power, or to 3R4F CS. Results showed that e-cig exposure decreased colon cell proliferation. Several other proliferative defects were observed in response to both e-cig and CS exposure, including up- and down-regulation of cyclin D1 protein levels in the ileum and colon, respectively. E-cig and CS exposure reduced myeloperoxidase activity in the ileum. In the colon, both exposures disrupted gene expression of cytokines and T cell transcription factors. For tight junction genes, ZO-1- and occludin-protein expression levels were reduced in the ileum and colon, respectively, by e-cig and CS exposure. The 16S sequencing of microbiota showed specific mild dysbiosis, according to the type of exposure. Overall, e-cig exposure led to altered proliferation, inflammation, and barrier function in both the ileum and colon, and therefore may be a gut hazard on par with conventional CS.","PeriodicalId":42356,"journal":{"name":"Journal of Xenobiotics","volume":"14 3","pages":"950-969"},"PeriodicalIF":6.8,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11270428/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0