Biotechnology Reports最新文献

{"title":"Design of a GFP reporter for splicing analysis in mammalian cells","authors":"Arthur T. Menezes,&nbsp;Helder Y. Nagasse,&nbsp;Hilan R. M. Lopes,&nbsp;Patricia P. Coltri","doi":"10.1016/j.btre.2025.e00887","DOIUrl":"10.1016/j.btre.2025.e00887","url":null,"abstract":"<div><div>Eukaryotic genes are formed by exons and introns. Pre-mRNA splicing promotes exon ligation and intron removal and is performed by a specialized macromolecular machinery named spliceosome, composed of five small ribonucleoprotein particles (snRNPs) and more than one hundred proteins. The activity of this complex is highly accurate due to the coordinated activity of its components. Altered splicing has been related to the development of several diseases, including neurodegenerative disorders, such as amyotrophic lateral sclerosis, and different types of cancer. Detailed understanding of splicing regulation in eukaryotic cells can be achieved using splicing reporter systems. We designed a reporter plasmid suitable for splicing analysis in cultured mammalian cells. Our reporter is based on GFP expression, and the splicing outcome can be easily visualized by fluorescence microscopy. We quantified splicing activity in two human cell lines, HEK-293T and MDA-MB-231, confirming its suitability for use in live cells in culture.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00887"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the potential biochemical effects of selected heterocyclic compounds as human Type-A γ-aminobutyric acid (GABA A) Modulator: An Insilico Approach","authors":"Abel Kolawole Oyebamiji ,&nbsp;Sunday Adewale Akintelu ,&nbsp;Oluwakemi Ebenezer ,&nbsp;Faith Eniola Olujinmi ,&nbsp;David O. Adekunle ,&nbsp;Adesoji Alani Olanrewaju ,&nbsp;Omowumi Temitayo Akinola ,&nbsp;Samson Olusegun Afolabi ,&nbsp;Ehimen Anastasia Erazua ,&nbsp;Ayodeji Arnold Olaseinde","doi":"10.1016/j.btre.2025.e00894","DOIUrl":"10.1016/j.btre.2025.e00894","url":null,"abstract":"<div><h3>Background</h3><div>Investigating the bioactivities of zuranolone derivatives as Type-A γ-aminobutyric acid inhibitors which will thereby down-regulate postpartum depression is considered a crucial study.</div></div><div><h3>Method</h3><div>This study is aimed at investigating the biochemical activities of 1-(2-((3R,5R,8R,9R,10S,13S,14S,17S)-3‑hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-oxoethyl)-1H-pyrazole-4-carbonitrile derivatives against type-A γ-aminobutyric acid (GABA A) which will thereby enhance the activity of γ-aminobutyric acid (GABA) in human central nervous system.</div></div><div><h3>Results</h3><div>In this work, series of computational tools such as Spartan 14, molecular operating environment software, Gromacs and Admetsar1 were explored and the studied compounds were subjected to this software which resulted to series of results. Vacuum was observed to have highest influence on highest occupied molecular orbital (E_H) of compound 1 and water as well as ethanol reduces its ability to donate electron to the nearby molecules. Also, the effect of water and ethanol were investigated on the studied compound via lowest unoccupied molecular orbital (E_L) and energy gap and the results were reported appropriately. The molecular docking investigation was carried out on the studied compounds and Type-A γ-aminobutyric acid (pdb id: 4cof) and the compounds 3 with calculated binding affinity value of -7.32433319kcal/mol as well as pi-H as the non-bonding interaction were observed which therefore confirm the potential ability of compound to inhibit the target than other studied compound. Also, compound 1 and reference compound were subjected to molecular dynamic simulation study and the actual binding energy for the selected compounds were obtained and reported.</div></div><div><h3>Conclusion</h3><div>Our findings from this work may open door for the design of several 1-(2-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(benzyloxy)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-oxoethyl)-1H-pyrazole-4-carbonitrile derivatives as potential Type-A γ-aminobutyric acid inhibitors.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00894"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells","authors":"Teresa Franchi-Mendes ,&nbsp;Marília Silva ,&nbsp;Maria Catarina Carreira ,&nbsp;Ana Luísa Cartaxo ,&nbsp;Paulo Vale ,&nbsp;Panagiotis Karakaidos ,&nbsp;Apostolos Klinakis ,&nbsp;Ana Fernandes-Platzgummer ,&nbsp;Cláudia L. da Silva","doi":"10.1016/j.btre.2025.e00878","DOIUrl":"10.1016/j.btre.2025.e00878","url":null,"abstract":"<div><h3>Introduction</h3><div>Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers.</div></div><div><h3>Materials &amp; Methods</h3><div>A gamma-irradiated human platelet lysate (HPL) supplement was used to develop a xeno(geneic)-free process for the isolation and scalable expansion of human bladder-derived SMC.</div></div><div><h3>Results</h3><div>Cells were isolated using an explant-based technique and expanded ex vivo, expressing typical SMC markers (α-SMA, desmin, caldesmon and SM22-α). Cell culture was successfully scaled-up using spinner flasks combined with plastic microcarriers, starting with a 2.8 × 10³ cells/cm² inoculum (i.e. 1 × 10⁶ cells). Cell-microcarrier adhesion rates exceeded 80% within 24 hours with fold expansion ranging from 3.5 to 16.8 after 7 days, dependent on donor variability. Harvested cells retained their SMC phenotype.</div></div><div><h3>Conclusions</h3><div>This xeno-free, GMP compliant process enables scalable manufacturing of human bladder-derived SMC while preserving cell identity, potentially advancing clinical applications in bladder engineering.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00878"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic copper and copper oxide nanoparticles to combat multidrug-resistant Staphylococcus aureus: Green synthesis, mechanisms, resistance, and future perspectives","authors":"Gamal M. El-Sherbiny,&nbsp;M.E. Shehata,&nbsp;Mohamed H. Kalaba","doi":"10.1016/j.btre.2025.e00896","DOIUrl":"10.1016/j.btre.2025.e00896","url":null,"abstract":"<div><div>Antimicrobial resistance has increased alarmingly in recent years, with the World Health Organization identifying multidrug-resistant <em>Staphylococcus aureus</em> as a particular threat to global public health due to its extensive resistance profile and associated high mortality rates. While various metal nanoparticles have been explored as antimicrobial agents, the specific advantages of biosynthesized copper nanoparticles against MDR <em>S. aureus</em> remain inadequately consolidated in the literature.</div></div><div><h3>Objective</h3><div>This review uniquely evaluates the emerging evidence for biosynthesized copper nanoparticles as a sustainable, cost-effective, and potentially alternative to conventional antibiotics against multidrug-resistant <em>S. aureus</em> strains.</div></div><div><h3>Methods</h3><div>We systematically analyzed current literature on green synthesis methods for copper and copper oxide nanoparticles, their characterization techniques, antimicrobial mechanisms, and efficacy against multidrug-resistant <em>S. aureus</em>, focusing on identifying knowledge gaps and future research directions.</div></div><div><h3>Results</h3><div>Unlike other metal nanoparticles, biosynthesized copper nanoparticles demonstrate significant antibacterial activity against multidrug-resistant <em>S. aureus</em> through multiple simultaneous mechanisms that bacteria try to develop resistance against. Their unique physicochemical properties enable enhanced bacterial elimination compared to conventional antibiotics and other metal nanoparticles, with minimal toxicity to mammalian cells at therapeutic concentrations. Our analysis further reveals the considerable potential of these nanoparticles to overcome existing biological barriers in infection sites that limit conventional therapies.</div></div><div><h3>Conclusion</h3><div>This broad assessment of biosynthesized copper nanoparticles shows strong potential as a therapy against MDR <em>S. aureus</em> and provides a foundation for future research to address antimicrobial resistance where current treatments fail.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00896"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and evaluation of rabies immunoglobulin extracted from chicken egg yolk","authors":"Vichununt Kerdput ,&nbsp;Vararut Yodkamol ,&nbsp;Mattika Sookprasong ,&nbsp;Wongsakorn Wongwadhunyoo ,&nbsp;Iyacoob Khunsri ,&nbsp;Promsin Masrinoul ,&nbsp;Trong Wisedchanwet ,&nbsp;Naphatsamon Uthailak ,&nbsp;Yanin Limpanont ,&nbsp;Onrapak Reamtong ,&nbsp;Poom Adisakwattana ,&nbsp;Charin Thawornkuno","doi":"10.1016/j.btre.2025.e00897","DOIUrl":"10.1016/j.btre.2025.e00897","url":null,"abstract":"<div><div>This study investigated the potential of using laying hens to produce rabies immunoglobulin (RIG) due to concerns about an African Horse Sickness outbreak in 2020. The hens were immunized with rabies vaccine, resulting in the production of Immunoglobulin Y (IgY) antibodies in their egg yolks. The study found that using polyethylene glycol 6000 (PEG) was the most effective method for extracting anti-rabies IgY from egg yolks in terms of amount (1.87 mg of IgY from one mL of egg yolk), purity (83 %), and cost-effectiveness. Further purification through affinity chromatography increased the purity to 95 %, as confirmed by 1D GeLC-MS/MS, showing a purity of up to 98 %. In addition, IgY extracted using PEG demonstrated the highest neutralizing activity compared to the caprylic acid and ammonium sulfate methods. It was estimated that only 8 eggs are needed to produce a vial of RIG with the same potency as equine RIG (1000 IU/vial).</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00897"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ partial reduction of biochar by overlaying a syngas stream","authors":"Valentin Chataigner ,&nbsp;Dominique Tarlet ,&nbsp;François Ricoul ,&nbsp;Jérôme Bellettre","doi":"10.1016/j.btre.2025.e00892","DOIUrl":"10.1016/j.btre.2025.e00892","url":null,"abstract":"<div><div>This work focuses on the partial reduction of a hot biochar by a hot syngas coming out of the partial oxidation zone in the Ariane® pyrolysis prototype. The patented geometry of this pyrolyser, with separate pyrolysis and partial oxidation zones, enables easy management of biochar and syngas flows. The aim is to increase the specific surface area of the biochar to achieve more agronomic specifications, while maintaining a high carbon content in the biochar. The experimental implementation of this partial reduction is achieved by adding a partial reduction zone at the bottom of the reactor, enabling direct contact between biochar and syngas. This part now enables biochar and syngas streams to be mixed in a high-temperature zone (<span><math><mrow><mo>&gt;</mo><msup><mn>500</mn><mo>∘</mo></msup><mi>C</mi></mrow></math></span>). The addition of this part increased the specific surface area of the biochar by <span><math><mrow><mn>250</mn><mspace></mspace><msup><mrow><mi>m</mi></mrow><mn>2</mn></msup><mo>/</mo><mi>g</mi></mrow></math></span> and preserved the carbon content of the biochar regardless of the biomass flow rate. Around <span><math><mrow><mn>20</mn><mspace></mspace><mo>%</mo></mrow></math></span> of the carbon in the biochar is consumed by the reduction and transferred from the biochar to the syngas. The syngas is thus enriched in particular in <span><math><msub><mi>H</mi><mn>2</mn></msub></math></span> and <span><math><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></math></span> and depleted in <span><math><mrow><mi>C</mi><msub><mi>H</mi><mn>4</mn></msub></mrow></math></span>, which opens up a wider range of development opportunities for this gas (biomethane, biohydrogen, biokerosene, cogeneration, etc.). Thanks to this activation method, a better compromise has been achieved (compared with the previous version of the prototype) between quality (specific surface area and carbon content) and quantity of biochar produced (anhydrous yield).</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00892"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the multifaceted roles of resuscitation-promoting factors in tuberculosis: Implications for diagnosis, vaccine development, and drug targeting","authors":"Gamze Tanriver ,&nbsp;Salman Ali Khan ,&nbsp;Artur Góra ,&nbsp;Novel N Chegou ,&nbsp;Shima Mahmoudi","doi":"10.1016/j.btre.2025.e00886","DOIUrl":"10.1016/j.btre.2025.e00886","url":null,"abstract":"<div><div>Tuberculosis (TB) remains a significant global health challenge, necessitating continued research efforts to unravel its complex pathogenesis and advance diagnostic, therapeutic, and preventive strategies. Resuscitation-promoting factors (Rpfs) are peptidoglycan-hydrolyzing enzymes that have gained attention due to their key roles in TB infection dynamics. This review aims to provide a comprehensive overview of Rpfs in TB, highlighting their immunological roles, diagnostic potential, and implications for vaccine development and drug targeting through both in silico and experimental approaches. Rpfs exhibit diverse roles in TB, influencing bacterial resuscitation from dormancy, and immune modulation through interactions with host immune cells, such as dendritic cells, and they are potential targets for novel diagnostic and therapeutic interventions. Their ability to stimulate immune responses, particularly interferon-γ production by T cells, underscores their potential as vaccine candidates against TB. Moreover, Rpfs represent promising targets for drug discovery, with inhibitors potentially disrupting bacterial resuscitation and growth.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00886"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of microbial contamination and biogenic amines formation in Ras cheese using Chlorella vulgaris extracts","authors":"Diaa A. Marrez","doi":"10.1016/j.btre.2025.e00895","DOIUrl":"10.1016/j.btre.2025.e00895","url":null,"abstract":"<div><div>The present study aimed to explore the impact of <em>C. vulgaris</em> aqueous and diethyl ether extracts on reducing microbial contamination and biogenic amines (BAs) formation in Ras cheese during ripening process. Phenolic profile of aqueous extract was quantified by high performance liquid chromatography (HPLC) and compounds in ether extract were determined using gas chromatography-mass spectrometry (GC–MS). Eleven compounds were detected in aqueous extract and catechin was the major (54.82 µg/g), while 14 compounds were detected in ether extract of which the main compounds hexadecane (14.26 %) and 9, 12-Octadecadienoic acid, methyl ester (9.61 %). <em>C. vulgaris</em> aqueous and ether extracts observed antibacterial activity against seven strains of foodborne bacteria with minimum inhibitory concentration (MIC) ranged from 0.33 to 1.43 mg/mL and antifungal activity against 9 strains of toxigenic fungi with MIC values from 0.42 to 1.74 mg/mL. Ras cheese treated with ether extract had the highest reduction in formation of tryptamine (93.4 %), β-phenylethylamine (86.4 %), Putrescine (88.3 %), Cadaverine (81.8 %), Histamine (62.9 %), Serotonin (92.1 %), Tyramine (87.2 %), Spermidine (80.8) and Spermine (86.8 %) compared to cheese treated with aqueous extract and control samples. Also, the microbial load (total bacterial count, yeasts and molds count, proteolytic bacterial count) in Ras cheese samples treated with <em>C. vulgaris</em> aqueous and diethyl ether extracts were lower than the control sample. Whereas, no growth of coliform group, Staphylococci and Salmonella were detected in treated samples and control. The microalga <em>C. vulgaris</em> extracts considered promising source from natural ingredients to reduce biogenic amines content and microbial load in cheese manufacturing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00895"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriophage-based biosensors technology: Materials, fabrications, efficiencies and shortcomings","authors":"Ihtisham Ul Haq ,&nbsp;Kashif Rahim ,&nbsp;Sajida Maryam ,&nbsp;Najeeba Parre Paker","doi":"10.1016/j.btre.2024.e00872","DOIUrl":"10.1016/j.btre.2024.e00872","url":null,"abstract":"<div><div>Ongoing research in biosensor technologies has led to advanced functional materials for healthcare diagnostics, and bacteriophages (phages), demonstrating exceptional utility due to their high specificity, accuracy, rapid, label-free, and wireless detection capabilities with minimal false-positive results. Phage-based-pathogen-detecting biosensors (PBPDBs) include surface plasmon resonance (SPR) biosensors, magnetoelastic (ME), electrochemical, and quartz crystal microbalance (QCM) biosensors. Commonly used substrates for PBPDBs are gold, silicon, glass, carbon-based materials, magnetic particles, and quantum dots. These substrates are chemically and physically modified to optimize phage orientation on sensor surfaces, enhancing bacterial capture. To address typical stability and issues encountered in traditional biosensor applications, phage particles and genetically modified phages are utilized to improve biosensor stability and increase detection efficacy while reducing assay time. Genetic modification in phages facilitated by CRISPR/Cas9 enables the tailoring of phages to target specific bacterial strains. This approach helps overcome the inherent specificity of phages and enables the detection of multiple pathogens in a single assay. Multiple pathogens can be detected through a single phage-based assay. This manuscript elucidates the fabrication methodologies and detection efficiencies of PBPDBs providing valuable insights into the development of practical, precise, and efficient biosensors for pathogen detection.</div></div><div><h3>Summary</h3><div>PBPDBs are emerging diagnostic tools for the detection of bacterial pathogens.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00872"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of microbial antagonistic activity and Quorum Sensing Signal Molecule (Cyclopeptides-DKPs and N-Acyl Homoserine Lactones) detection in bacterial strains obtained from avocado thrips (Thysanoptera: Thripidae)","authors":"A.N. Pereira-Bazurdo ,&nbsp;G.E. Cadavid-Restrepo ,&nbsp;R.E. Arango-Isaza ,&nbsp;C.X. Moreno-Herrera","doi":"10.1016/j.btre.2024.e00866","DOIUrl":"10.1016/j.btre.2024.e00866","url":null,"abstract":"<div><div>The control of avocado pests and diseases heavily relies on the use of several types of pesticides, some of which are strictly monitored or not internationally accepted. New sources of bioactive molecules produced by phytopathogen-inhibiting microorganisms offer an excellent alternative for the control of pests and diseases. This study explores the potential antagonistic action against phytopathogenic microorganisms, using bacterial strains obtained from avocado thrips. In addition, we detected and identified quorum sensing (QS) signaling molecules that are related to virulence factors and antibiotic production. The results showed that <em>Bacillus, Pantoea</em>, and <em>Serratia</em> strains exhibited antagonism against five fungal phytopathogens. Additionally, some bacteria also produce specific signaling molecules like N-3-(oxododecanoyl)-l-homoserine lactone (OdDHL), N-(3-oxo)-hexanoyl l-HL (OHHL), 4‑hydroxy-2-heptylquinoline (HHQ) or 2-heptyl-3,4-dihydroxyquinoline (PQS, Pseudomonas quinolone signal), cyclo(L-Phe-l-Pro), and cyclo(L-Pro-l-Tyr, which might give them antimicrobial properties. This research explores the biotechnological potential of these bacteria in fighting the diseases affecting avocados in Colombia.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00866"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}