Biotechnology Reports最新文献_第7页

Biophysical characterization of α-glucan nanoparticles encapsulating feruloylated soy glycerides (FSG) α-葡聚糖纳米颗粒包封阿魏酰化大豆甘油酯的生物物理特性研究

Biotechnology Reports Pub Date : 2023-11-02 DOI: 10.1016/j.btre.2023.e00817

Kervin O. Evans , David L. Compton , Christopher D. Skory , Michael Appell

{"title":"Biophysical characterization of α-glucan nanoparticles encapsulating feruloylated soy glycerides (FSG)","authors":"Kervin O. Evans , David L. Compton , Christopher D. Skory , Michael Appell","doi":"10.1016/j.btre.2023.e00817","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00817","url":null,"abstract":"<div>Water insoluble α-glucans that were enzymatically synthesized using glucansucrase that was cloned from Leuconostoc mesenteroides NRRL B-1118 were previously shown to form nanoparticles via high pressure homogenization. These α-glucan nanoparticles were previously shown capable of encapsulating a small hydrophobic molecule. This work demonstrates that the same α-glucan can be formed into nanoparticles that encapsulate feruloylated soy glycerides from modified soybean oil, a product of interest to the cosmetic and skin care industries because of the UV absorbance and antioxidant properties of the feruloyl moiety. It is demonstrated that the feruloylated soy glyceride/α-glucan nanoparticles have distinct size, zeta potential and thermal profiles from that of nanoparticles made from α-glucan alone or feruloylated soy glyceride alone. Thermal analysis also demonstrates the release of feruloylated soy glycerides from the α-glucan nanoparticles.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00817"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000371/pdfft?md5=525ecfb7e76fc645ca899088a354d3e0&pid=1-s2.0-S2215017X23000371-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92135913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemicals reduced growth, sporulation and conidial dimensions of Fusarium verticillioides, cause of fumonisin contamination in maize grains 植物化学物质降低玉米中伏马菌素污染的原因——黄萎病病菌的生长、产孢和分生孢子尺寸

Biotechnology Reports Pub Date : 2023-10-30 DOI: 10.1016/j.btre.2023.e00819

Habtamu Terefe , Getnet Yitayih , Getachew G. Mengesha

{"title":"Phytochemicals reduced growth, sporulation and conidial dimensions of Fusarium verticillioides, cause of fumonisin contamination in maize grains","authors":"Habtamu Terefe , Getnet Yitayih , Getachew G. Mengesha","doi":"10.1016/j.btre.2023.e00819","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00819","url":null,"abstract":"<div>The objective of the study was to evaluate the effectiveness of methanolic extracts of plants against radial growth and spore dimensions of Fusarium verticillioides. Leaf extracts of 25 plants were tested against the fungus. Of which, thirteen extracts were potent against the fungus and evaluated using food poising technique. Growth was evaluated on PDA medium amended with the extracts at 5 mg ml–1. Control treatments included plates without (negative) extracts and with synthetic (positive) fungicide. Spore dimension was determined using PDB. The results showed T. vulgaris extract completely inhibited mycelial growth of the fungus as equivalent as the fungicide. Similarly, G. parviflora, C. citratus, R. officinalis, R. chalepensis, and Agave sp. also recorded growth reductions ranging from 71.04 to 81.35 % at day seven. In addition, extracts of Agave sp., C. citratus and T. vulgaris did not support sporulation. Overall, the results indicate that T. vulgaris extracts could be safe source of bioactive chemicals to control F. verticillioides.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00819"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000395/pdfft?md5=1cc2d77ef7caffec3c8425d3d537fc74&pid=1-s2.0-S2215017X23000395-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92044482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the polysaccharide production by the microalgae C-1509 Nannochloris sp. Naumann 微藻C-1509 Nannochloris sp. Naumann生产多糖的研究

Biotechnology Reports Pub Date : 2023-10-30 DOI: 10.1016/j.btre.2023.e00818

Olga Babich , Svetlana Ivanova , Aleksandr Tupitsyn , Aleksandr Vladimirov , Elena Nikolaeva , Archana Tiwari , Ekaterina Budenkova , Egor Kashirskikh , Veronika Anokhova , Philippe Michaud , Stanislav Sukhikh

{"title":"Study of the polysaccharide production by the microalgae C-1509 Nannochloris sp. Naumann","authors":"Olga Babich , Svetlana Ivanova , Aleksandr Tupitsyn , Aleksandr Vladimirov , Elena Nikolaeva , Archana Tiwari , Ekaterina Budenkova , Egor Kashirskikh , Veronika Anokhova , Philippe Michaud , Stanislav Sukhikh","doi":"10.1016/j.btre.2023.e00818","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00818","url":null,"abstract":"<div>Biologically active compounds, including polysaccharides isolated from microalgae, have various properties. Although Nannochloropsis spp. have the potential to produce secondary metabolites important for biotechnology, only a small part of the research on these microalgae has focused on their ability to produce polysaccharide fractions. This study aims to evaluate the physicochemical growth factors of Nannochloropsis spp. microalgae, which ensure the maximum accumulation of polysaccharides, as well as to optimize the parameters of polysaccharide extraction. The optimal nutrient medium composition was selected to maximize biomass and polysaccharide accumulation. The significance of selecting the extraction module and extraction temperature regime, as well as the cultivation conditions (temperature and active acidity value) is emphasized. Important chemical components of polysaccharides responsible for their biological activity were identified</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00818"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000383/pdfft?md5=4aa5b341e850250652f19ea74d918eb3&pid=1-s2.0-S2215017X23000383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91963088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the biosynthesis of silver nanoparticles using bacterial extracts and their antimicrobial potential 细菌提取物生物合成纳米银的优化及其抑菌潜力

Biotechnology Reports Pub Date : 2023-10-29 DOI: 10.1016/j.btre.2023.e00816

Iván Solís-Sandí , Sara Cordero-Fuentes , Reinaldo Pereira-Reyes , José Roberto Vega-Baudrit , Diego Batista-Menezes , Gabriela Montes de Oca-Vásquez

{"title":"Optimization of the biosynthesis of silver nanoparticles using bacterial extracts and their antimicrobial potential","authors":"Iván Solís-Sandí , Sara Cordero-Fuentes , Reinaldo Pereira-Reyes , José Roberto Vega-Baudrit , Diego Batista-Menezes , Gabriela Montes de Oca-Vásquez","doi":"10.1016/j.btre.2023.e00816","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00816","url":null,"abstract":"<div>In the present study, silver nanoparticles (AgNPs) were biosynthesized using the supernatant and the intracellular extract of Cupriavidus necator, Bacillus megaterium, and Bacillus subtilis. The characterization of the AgNPs was carried out using UV–Vis spectroscopy, FTIR, DLS and TEM. Resazurin microtiter-plate assay was used to determine the antimicrobial action of AgNPs against Escherichia coli. UV–Visible spectra showed peaks between 414 and 460 nm. TEM analysis revealed that the synthesized AgNPs showed mostly spherical shapes. DLS results determined sizes from 20.8 to 118.4 nm. The highest antimicrobial activity was obtained with the AgNPs synthesized with supernatant rather than those using the intracellular extract. Therefore, it was determined that the bacterial species, temperature, pH, and type of extract (supernatant or intracellular) influence the biosynthesis. This synthesis thus offers a simple, environmentally friendly, and low-cost method for the production of AgNPs, which can be used as antibacterial agents.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00816"},"PeriodicalIF":0.0,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X2300036X/pdfft?md5=d77dd86f44ffb8e55f3c254c648e6ec8&pid=1-s2.0-S2215017X2300036X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92044481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, screening and identification of ethanol producing yeasts from Ethiopian fermented beverages 埃塞俄比亚发酵饮料中产乙醇酵母的分离、筛选和鉴定。

Biotechnology Reports Pub Date : 2023-10-04 DOI: 10.1016/j.btre.2023.e00815

Dagnew Bitew , Anteneh Tesfaye , Berhanu Andualem

引用次数: 0

Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection 电穿孔和显微注射在具有完整绒毛膜的斑马鱼胚胎中的转基因表达

Biotechnology Reports Pub Date : 2023-10-02 DOI: 10.1016/j.btre.2023.e00814

Nusrat Tazin , Christopher Jordon Lambert , Raheel Samuel , Tamara J. Stevenson , Joshua L. Bonkowsky , Bruce K. Gale

{"title":"Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection","authors":"Nusrat Tazin , Christopher Jordon Lambert , Raheel Samuel , Tamara J. Stevenson , Joshua L. Bonkowsky , Bruce K. Gale","doi":"10.1016/j.btre.2023.e00814","DOIUrl":"10.1016/j.btre.2023.e00814","url":null,"abstract":"<div>Electroporation is regularly used to deliver agents into cells, including transgenic materials, but it is not used for mutating zebrafish embryos due to the lack of suitable systems, information on appropriate operating parameters, and the challenges posed by the protective chorion. Here, a novel method for gene delivery in zebrafish embryos was developed by combining microinjection into the space between the chorion and the embryo followed by electroporation. This method eliminates the need for chorion removal and injecting into the space between the chorion and embryo eliminates the need for finding and identifying key cell locations before performing an injection, making the process much simpler and more automatable. We also developed a microfluidic electroporation system and optimized electric pulse parameters for transgenesis of embryos. The study provided a novel method for gene delivery in zebrafish embryos that can be potentially implemented in a high throughput transgenesis or mutagenesis system.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00814"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/32/ac/main.PMC10569972.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and physio-biochemical responses under heat stress in cotton: Overview 棉花在热胁迫下的形态和生理生化反应

Biotechnology Reports Pub Date : 2023-09-14 DOI: 10.1016/j.btre.2023.e00813

Aamir Ali Abro , Muhammad Anwar , Muhammad Umer Javwad , Mjie Zhang , Fang Liu , Raimundo Jiménez-Ballesta , Ehab A. A. Salama , Mohamed A. A. Ahmed

{"title":"Morphological and physio-biochemical responses under heat stress in cotton: Overview","authors":"Aamir Ali Abro , Muhammad Anwar , Muhammad Umer Javwad , Mjie Zhang , Fang Liu , Raimundo Jiménez-Ballesta , Ehab A. A. Salama , Mohamed A. A. Ahmed","doi":"10.1016/j.btre.2023.e00813","DOIUrl":"10.1016/j.btre.2023.e00813","url":null,"abstract":"<div>Cotton is an important cash crop in addition to being a fiber commodity, and it plays an essential part in the economies of numerous nations. High temperature is the most critical element affecting its yield from fertilization to harvest. The optimal temperature for root formation is 30 C -35 °C; however, root development ends around 40 °C. Increased temperature, in particular, influences different biochemical and physiological processes associated with cotton plant, resulting in low seed cotton production. Many studies in various agroecological zones used various agronomic strategies and contemporary breeding techniques to reduce heat stress and improve cotton productivity. To attain desired traits, cotton breeders should investigate all potential possibilities, such as generating superior cultivars by traditional breeding, employing molecular techniques and transgenic methods, such as using genome editing techniques. The main objective of this review is to provide the recent information on the environmental factors, such as temperature, heat and drought, influence the growth and development, morphology and physio-chemical alteration associated with cotton. Furthermore, recent advancement in cotton breeding to combat the serious threat of drought and heat stress.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00813"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/28/c0/main.PMC10582760.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vegetative insecticidal protein (Vip3A) production by Bacillus thuringiensis Bt294 and its efficacy against Lepidopteran pests (Spodoptera exigua) 苏云金芽孢杆菌Bt294生产的植物性杀虫蛋白Vip3A及其对鳞翅目害虫的防治效果

Biotechnology Reports Pub Date : 2023-09-12 DOI: 10.1016/j.btre.2023.e00812

Pumin Nutaratat , Borworn Werapan , Netnapa Phosrithong , Chutchanun Trakulnaleamsai , Amporn Rungrod , Mongkon Utamatho , Sumarin Soonsanga , Boonhiang Promdonkoy , Kwanruthai Malairuang , Wai Prathumpai

{"title":"Vegetative insecticidal protein (Vip3A) production by Bacillus thuringiensis Bt294 and its efficacy against Lepidopteran pests (Spodoptera exigua)","authors":"Pumin Nutaratat , Borworn Werapan , Netnapa Phosrithong , Chutchanun Trakulnaleamsai , Amporn Rungrod , Mongkon Utamatho , Sumarin Soonsanga , Boonhiang Promdonkoy , Kwanruthai Malairuang , Wai Prathumpai","doi":"10.1016/j.btre.2023.e00812","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00812","url":null,"abstract":"<div>A vegetative insecticidal protein, Vip3A, is highly active against lepidopteran pests, which are the most important pests in most tropical countries. An important aspect of the successful commercial production of this bacterial insecticide is the development of bacterial culture media that maximize the titres of this protein and cost reduction. This study aimed to investigate and optimize Vip3A production by Bacillus thuringiensis Bt294 using statistical methods and 3-step sequential approaches. The experimental design showed that the production of Vip3A was maximized to 300 mg/L when the bacterium was cultivated in medium composed of 5.05 g/L glycerol, 49.17 g/L soytone, 30.05 g/L casein hydrolysate, 1.99 g/L CaCl2.2H2O, 7.5 mg/L CuSO4, 15 mg/L MnSO4.H2O, 9.4 g/L K2HPO4, 2.2 g/L KH2PO4, 0.2 g/L MgSO4.7H2O, 5 g/L yeast extract, 2.5 mg/L NiCl2.6H2O and 3 mL/L vitamin solution. B. thuringiensis Bt294 Vip3A toxin was highly toxic to Spodoptera exigua with LC50 values of 187.1 ng/cm2 at 7 days. This result demonstrated that a high titre of Vip3A produced by B. thuringiensis Bt294 will be useful as a biological control agent. This optimization will allow production to be scaled up for commercial production in the future.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00812"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50173530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibition of bacterial biofilms by the snake venom proteome 蛇毒蛋白质组对细菌生物膜的抑制作用

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00810

Neyaz A. Khan , Fernanda G. Amorim , John P. Dunbar , Dayle Leonard , Damien Redureau , Loïc Quinton , Michel M. Dugon , Aoife Boyd

{"title":"Inhibition of bacterial biofilms by the snake venom proteome","authors":"Neyaz A. Khan , Fernanda G. Amorim , John P. Dunbar , Dayle Leonard , Damien Redureau , Loïc Quinton , Michel M. Dugon , Aoife Boyd","doi":"10.1016/j.btre.2023.e00810","DOIUrl":"10.1016/j.btre.2023.e00810","url":null,"abstract":"<div>Snake venoms possess a range of pharmacological and toxicological activities. Here we evaluated the antibacterial and anti-biofilm activity against methicillin-susceptible and methicillin-resistant Staphylococcus aureus (MSSA and MRSA) of venoms from the Samar spitting cobra Naja samarensis and the Puff adder Bitis arietans. Both venoms prevented biofilm production by pathogenic S. aureus in a growth-independent manner, with the B. arietans venom being most potent. Fractionation showed the active molecule to be heat-labile and >10 kDa in size. Proteomic profiles of N. samarensis venom revealed neurotoxins and cytotoxins, as well as an abundance of serine proteases and three-finger toxins, while serine proteases, metalloproteinases and C-lectin types were abundant in B. arietans venom. These enzymes may have evolved to prevent bacteria colonising the snake venom gland. From a biomedical biotechnology perspective, they have valuable potential for anti-virulence therapy to fight antibiotic resistant microbes.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00810"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9973050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A high throughput screening process and quick isolation of novel lignin-degrading microbes from large number of natural biomasses 一种高通量筛选方法及从大量天然生物质中快速分离新型木质素降解微生物

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00809

Nadia Sufdar Ali , Fang Huang , Wensheng Qin , Trent Chunzhong Yang PhD

{"title":"A high throughput screening process and quick isolation of novel lignin-degrading microbes from large number of natural biomasses","authors":"Nadia Sufdar Ali , Fang Huang , Wensheng Qin , Trent Chunzhong Yang PhD","doi":"10.1016/j.btre.2023.e00809","DOIUrl":"10.1016/j.btre.2023.e00809","url":null,"abstract":"<div>High throughput screening approaches can significantly speed up the identification of novel enzymes from natural microbial consortiums. A two-step high throughput screening process was proposed and explored to screen lignin-degrading microorganisms. By employing this modified culture enrichment method and screening based on enzyme activity, a total of 82 bacterial and 46 fungal strains were isolated from fifty decayed wood samples (100 liquid cultures) collected from the banks of the Ottawa River in Canada. Among them, ten bacterial and five fungal strains were selected and identified based on their high laccase activities by 16S rDNA and ITS gene sequencing, respectively. The study identified bacterial strains from various genera including Serratia, Enterobacter, Raoultella, and Bacillus, along with fungal counterparts including Mucor, Trametes, Conifera and Aspergillus. Moreover, Aspergillus sydowii (AORF21), Mucor sp. (AORF43), Trametes versicolor (AORF3) and Enterobacter sp. (AORB55) exhibited xylanase and β- glucanase activities in addition to laccase production. The proposed approach allowed for the quick identification of promising consortia and enhanced the chance of isolating desired strains based on desired enzyme activities. This method is not limited to lignocellulose and lignin-degrading microorganisms but can be applied to identify novel microbial strains and enzymes from different natural samples.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00809"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/c3/main.PMC10423689.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10012649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0