Biotechnology Reports最新文献_第10页

Development and scale-up of rVSV-SARS-CoV-2 vaccine process using single use bioreactor 使用一次性生物反应器开发和扩大rVSV-SARS-CoV-2疫苗工艺

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2023.e00782

Christopher Ton , Victoria Stabile , Elizabeth Carey , Adam Maraikar , Travis Whitmer , Samantha Marrone , Nelson Lee Afanador , Igor Zabrodin , Greeshma Manomohan , Melissa Whiteman , Carl Hofmann

{"title":"Development and scale-up of rVSV-SARS-CoV-2 vaccine process using single use bioreactor","authors":"Christopher Ton , Victoria Stabile , Elizabeth Carey , Adam Maraikar , Travis Whitmer , Samantha Marrone , Nelson Lee Afanador , Igor Zabrodin , Greeshma Manomohan , Melissa Whiteman , Carl Hofmann","doi":"10.1016/j.btre.2023.e00782","DOIUrl":"10.1016/j.btre.2023.e00782","url":null,"abstract":"<div>The outbreak of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes the Coronavirus Disease 2019 (COVID-19) has spread through the globe at an alarming speed. The disease has become a global pandemic affecting millions of people and created public health crises worldwide. Among many efforts to urgently develop a vaccine against this disease, we developed an industrial-scale closed, single use manufacturing process for V590, a vaccine candidate for SARS-CoV-2. V590 is a recombinant vesicular stomatitis virus (rVSV) genetically engineered to express SARS-CoV-2 glycoprotein. In this work, we describe the development and optimization of serum-free microcarrier production of V590 in Vero cells in a closed system. To achieve the maximum virus productivity, we optimized pH and temperature during virus production in 3 liters (L) bioreactors. Virus productivity was improved (by ∼1 log) by using pH 7.0 and temperature at 34.0 °C. The optimal production condition was successfully scaled up to a 2000 L Single Use Bioreactor (SUB), producing a maximum virus titer of ∼1.0e+7 plaque forming units (PFU)/mL. Further process intensification and simplification, including growing Vero cells at 2 gs per liter (g/L) of Cytodex-1 Gamma microcarriers and eliminating the media exchange (MX) step prior to infection helped to increase virus productivity by ∼2-fold.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00782"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/44/main.PMC9841742.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10618693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Biogenic selenium nanoparticles with antifungal activity against the wood-rotting fungus Oligoporus pelliculosus 生物硒纳米颗粒对腐木真菌薄膜寡孢菌的抗真菌活性

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2023.e00787

Micaela Pescuma , Francisca Aparicio , Roberto D. Zysler , Enio Lima , Claudia Zapata , Jorge A. Marfetán , M.Laura Vélez , Omar F. Ordoñez

{"title":"Biogenic selenium nanoparticles with antifungal activity against the wood-rotting fungus Oligoporus pelliculosus","authors":"Micaela Pescuma , Francisca Aparicio , Roberto D. Zysler , Enio Lima , Claudia Zapata , Jorge A. Marfetán , M.Laura Vélez , Omar F. Ordoñez","doi":"10.1016/j.btre.2023.e00787","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00787","url":null,"abstract":"<div>Selenium nanoparticles (SeNPs) have antimicrobial and antifungal activity. SeNPs using Se resistant bacteria is a low cost and eco-friendly technology. Fungal contamination of wood during drying is one of the main causes of economic losses in the wood industry. The bacterium Delftia sp. 5 resistance to Se and its ability to produce SeNPs able to inhibit the growth of the wood brown-rotting fungus Oligoporus pelliculosus was analyzed. The strain showed an optimal SeNPs production when selenite concentration was 160 mg L−1. The SeNPs were spherical with an average size 192.33 ± 8.6 nm and a zeta potential of -41.4 ± 1.3 nm. The SeNPs produced by Delftia sp. 5 (33.6 ± 0.1 mg L−1 Se) inhibited the growth of O. pelliculosus in agar plates and in Nothofagus pumilio (Lenga) wood samples. Delftia sp. 5 SeNPs could be used for embedding lenga wood prior to drying for preventing the growth of the deteriorating fungi O. pelliculosus.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00787"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Immunogenicity and efficacy of recombinant subunit SARS-CoV-2 vaccine candidate in the Syrian hamster model 重组亚单位严重急性呼吸系统综合征冠状病毒2型候选疫苗在叙利亚仓鼠模型中的免疫原性和疗效

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2022.e00779

Balamurugan Shanmugaraj , Narach Khorattanakulchai , Weena Paungpin , Yada Akkhawattanangkul , Suwimon Manopwisedjaroen , Arunee Thitithanyanont , Waranyoo Phoolcharoen

{"title":"Immunogenicity and efficacy of recombinant subunit SARS-CoV-2 vaccine candidate in the Syrian hamster model","authors":"Balamurugan Shanmugaraj , Narach Khorattanakulchai , Weena Paungpin , Yada Akkhawattanangkul , Suwimon Manopwisedjaroen , Arunee Thitithanyanont , Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2022.e00779","DOIUrl":"10.1016/j.btre.2022.e00779","url":null,"abstract":"<div>SARS-CoV-2 causes devastating impact on the human population and has become a major public health concern. The frequent emergence of SARS-CoV-2 variants of concern urges the development of safe and efficacious vaccine against SARS-CoV-2 variants. We developed a candidate vaccine Baiya SARS-CoV-2 Vax 1, based on SARS-CoV-2 receptor-binding domain (RBD) by fusing with the Fc region of human IgG. The RBD-Fc fusion was produced in Nicotiana benthamiana. Previously, we reported that this plant-produced vaccine is effective in inducing immune response in both mice and non-human primates. Here, the efficacy of our vaccine candidate was tested in Syrian hamster challenge model. Hamsters immunized with two intramuscular doses of Baiya SARS-CoV-2 Vax 1 induced neutralizing antibodies against SARS-CoV-2 and protected from SARS-CoV-2 challenge with reduced viral load in the lungs. These preliminary results demonstrate the ability of plant-produced subunit vaccine Baiya SARS-CoV-2 Vax 1 to provide protection against SARS-CoV-2 infection in hamsters.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00779"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/4d/main.PMC9744481.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10413552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Non-denatured yak type I collagen accelerates sunburned skin healing by stimulating and replenishing dermal collagen 非变性牦牛I型胶原蛋白通过刺激和补充真皮胶原蛋白加速晒伤皮肤的愈合

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2022.e00778

Caihong Fu , Shuangni Shi , Jing Tian , Hong Gu , Linyan Yao , Jianxi Xiao

{"title":"Non-denatured yak type I collagen accelerates sunburned skin healing by stimulating and replenishing dermal collagen","authors":"Caihong Fu , Shuangni Shi , Jing Tian , Hong Gu , Linyan Yao , Jianxi Xiao","doi":"10.1016/j.btre.2022.e00778","DOIUrl":"10.1016/j.btre.2022.e00778","url":null,"abstract":"<div>Sunburn is one of the most common skin lesions caused by excessive UV exposure, and its incidence is highly correlated with the risks of skin cancer. A variety of drugs including corticosteroids and NSAIDs have been developed to treat acute sunburn, however, they have raised severe concerns such as poor healing efficacy and long recovery time. We have for the first time extracted non-denatured type I collagen from yak hide, which displays a canonical triple helical structure with melting temperature of 42.7 °C. The highly pure yak collagen type I (YCI) self-assembles to form well-ordered nanofibers with periodic d-bands. YCI is highly biocompatible, and it significantly promotes the proliferation and adhesion of HFF-1 cells. The sunburn healing effects of YCI has been investigated using acute skin injury mouse model. Histological analysis shows that 4 days’ treatment of YCI has resulted in the recovery of sunburned mice skin to a healthy state, indicated by pronounced acceleration of epithelization and collagen deposition. The collagen volume fraction as well as the hydroxyproline (Hyp) content of YCI-treated sunburned skin have been found to be greatly increased, confirming the enhanced regeneration of collagen. YCI creams and dressings have also shown superior healing capacity of sunburn by remarkably shortening the recovery time. Notably, the denatured collagen-targeted staining results indicated a large quantity of denatured collagen in sunburned mice, which became substantially reduced after the YCI treatment. FITC-labeled YCI has been further found to penetrate into the dermis of sunburned mice. The highly biocompatible and bioactive non-denatured YCI provides an improved treatment of sunburn, indicating very promising applications of YCI in cosmetics and dermatology.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00778"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10451109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cu2O nanoparticles synthesized by green and chemical routes, and evaluation of their antibacterial and antifungal effect on functionalized textiles 绿色和化学途径合成Cu2O纳米粒子及其对功能化纺织品的抗菌和抗真菌作用评价

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2023.e00785

David Asmat-Campos , Gabriela Montes de Oca-Vásquez , Jesús Rojas-Jaimes , Daniel Delfín-Narciso , Luisa Juárez-Cortijo , Renny Nazario-Naveda , Diego Batista Menezes , Reinaldo Pereira , Marcos Simbrón de la Cruz

{"title":"Cu2O nanoparticles synthesized by green and chemical routes, and evaluation of their antibacterial and antifungal effect on functionalized textiles","authors":"David Asmat-Campos , Gabriela Montes de Oca-Vásquez , Jesús Rojas-Jaimes , Daniel Delfín-Narciso , Luisa Juárez-Cortijo , Renny Nazario-Naveda , Diego Batista Menezes , Reinaldo Pereira , Marcos Simbrón de la Cruz","doi":"10.1016/j.btre.2023.e00785","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00785","url":null,"abstract":"<div>The potential for the application of metal-containing nanomaterials at the nanoscale promotes the opportunity to search for new methods for their elaboration, with special attention to those sustainable methods. In response to these challenges, we have investigated a new method for green synthesis of cuprous oxide nanoparticles (Cu2O NPs) using Myrciaria dubia juice as an organic reductant and, comparing it with chemical synthesis, evaluating in both cases the influence of the volume of the organic (juice) and chemical (ascorbic acid) reductants, for which a large number of techniques such as spectrophotometry, EDX spectrometry, TEM, SEM, DLS, FTIR spectroscopy have been used. Likewise, the nanomaterial with better morphological characteristics, stability, and size homogeneity has been applied in the functionalization of textiles by means of in situ and post-synthesis impregnation methods. The success of the synthesis process has been demonstrated by the antimicrobial activity (bacteria and fungi) of textiles impregnated with Cu2O NPs.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00785"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The potency of herbal extracts and its green synthesized nanoparticle formulation as antibacterial agents against Streptococcus mutans associated biofilms 草药提取物及其绿色合成纳米颗粒制剂对变形链球菌相关生物膜的抗菌作用

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2022.e00777

Kulwadee Karnjana , Juntamanee Jewboonchu , Nattisa Niyomtham , Paveen Tangngamsakul , Kingkan Bunluepuech , Lavnaya Goodla , Auemphon Mordmuang

{"title":"The potency of herbal extracts and its green synthesized nanoparticle formulation as antibacterial agents against Streptococcus mutans associated biofilms","authors":"Kulwadee Karnjana , Juntamanee Jewboonchu , Nattisa Niyomtham , Paveen Tangngamsakul , Kingkan Bunluepuech , Lavnaya Goodla , Auemphon Mordmuang","doi":"10.1016/j.btre.2022.e00777","DOIUrl":"https://doi.org/10.1016/j.btre.2022.e00777","url":null,"abstract":"<div>This study aims to determine the effects of the extracts of Streblus asper, Cymbopogon citratus, Syzygium aromaticum and its formulation of green synthesized silver nanoparticle (AgNPs) on Streptococcus mutans growth and biofilm formation. The ethanolic extracts of S. asper, C. citratus, S. aromaticum, and a mix of the three herbs demonstrated antibacterial activity against S. mutans isolates by reducing bacterial biofilm formation and decreasing bacterial cell surface hydrophobicity. The formulated AgNPs from the ethanolic extracts could enhance the antibacterial activities of the plant extracts. Molecular docking found the best interaction between luteolin isolated from C. citratus and glucosyltransferase protein (GtfB), assuming the promising anti-biofilm activity. The scanning electron microscopy revealed morphological changes in the biofilm structure and a significant decrease in the biofilm area of the AgNPs treated. The study suggested that the extracts and its application could be used as natural alternative agents with multi-action against S. mutans infections.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00777"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of radiation responsive RBC membrane associated proteins (RMAPs) in whole-body γ-irradiated New Zealand white rabbits γ辐射新西兰大白鼠辐射反应性红细胞膜相关蛋白的鉴定

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2023.e00783

Jubilee Purkayastha , Priyanka Grover , Prabuddho Mukherjee , Kamendra Kumar , Sudhir Chandna

{"title":"Identification of radiation responsive RBC membrane associated proteins (RMAPs) in whole-body γ-irradiated New Zealand white rabbits","authors":"Jubilee Purkayastha , Priyanka Grover , Prabuddho Mukherjee , Kamendra Kumar , Sudhir Chandna","doi":"10.1016/j.btre.2023.e00783","DOIUrl":"10.1016/j.btre.2023.e00783","url":null,"abstract":"<div>This study is aimed to identify radiation-responsive RBC Membrane Associated Proteins (RMAPs) in Rabbits in vivo. Male New Zealand White rabbits were exposed to a single acute total body γ-radiation dose of 2 Gy at a dose rate of 0.746 Gy/min. Following this, at early time points of 6 h till the 7 d, RMAPs were collected and analyzed by MALDI-TOF-MS. Bioinformatics analysis was conducted to explore the biological functions of these proteins. Based on fold change, radiation responsiveness, GO, pathway enrichment, and hub position in the PPI network, we identified seven RMAPs as potential biomarker candidates viz., PVALB, PRKCB, GPD1, CP2G1, CSNK2B, ATP1B1, TPI1. As per KEGG enrichment, most of the proteins were implicated in cellular radiation response, oxidative damage, DNA repair, apoptosis, immune response, and cell signaling. This study forms the foundation for RMAPs-based Proteomic strategies for high throughput radiation bio-dosimetry for triage in the case of a radiological/nuclear incident.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00783"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9143406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coproduction of lipids and carotenoids by the novel green alga Coelastrella sp. depending on cultivation conditions 新型绿藻Coelastrella sp.在不同培养条件下共生产脂质和类胡萝卜素

Biotechnology Reports Pub Date : 2023-03-01 DOI: 10.1016/j.btre.2022.e00769

Mizuki Saito , Haruka Watanabe , Mitsuki Sasaki , Madoka Ookubo , Takashi Yarita , Masakazu Shiraiwa , Munehiko Asayama

{"title":"Coproduction of lipids and carotenoids by the novel green alga Coelastrella sp. depending on cultivation conditions","authors":"Mizuki Saito , Haruka Watanabe , Mitsuki Sasaki , Madoka Ookubo , Takashi Yarita , Masakazu Shiraiwa , Munehiko Asayama","doi":"10.1016/j.btre.2022.e00769","DOIUrl":"https://doi.org/10.1016/j.btre.2022.e00769","url":null,"abstract":"<div>A novel green alga Coelastrella sp. D3–1 was isolated, and its unique and significant lipid and carotenoid coproduction capability was characterised depending on cultivation conditions. The main component of produced lipids was triacylglycerol under nutrient depletion conditions, in which fatty-methyl-esters made up 20−44% of the dry cell weight (DCW) and consisted of abundant C16:0 and C18:1 fatty acids. The red (orange)-stage cells also produced a large portion of carotenoids (38.5% of the DCW) involving echinenone, canthaxanthin, and astaxanthin as major components accumulated over only 5–6 days under optimal conditions. Stress tests revealed resistance of the cells to pH 2–11, high temperatures (40–60 °C), ultraviolet irradiation, drought, and H2O2 treatment, thereby showing a robust nature. Both green- and red (orange)-stage cell extracts also showed antioxidant and anti-inflammatory abilities, implying that they have significant functions as useful biorefinery materials.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00769"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae 酵母在GAL启动子下无诱导剂强表达的新平台宿主

Biotechnology Reports Pub Date : 2022-12-01 DOI: 10.1016/j.btre.2022.e00763

Mi-Jin Kim , Bong Hyun Sung , Hyun-Joo Park , Jung-Hoon Sohn , Jung-Hoon Bae

{"title":"A new platform host for strong expression under GAL promoters without inducer in Saccharomyces cerevisiae","authors":"Mi-Jin Kim , Bong Hyun Sung , Hyun-Joo Park , Jung-Hoon Sohn , Jung-Hoon Bae","doi":"10.1016/j.btre.2022.e00763","DOIUrl":"10.1016/j.btre.2022.e00763","url":null,"abstract":"<div>The gal80 mutant of yeast Saccharomyces cerevisiae is used for the constitutive expression under strong GAL promoters without galactose induction. To enhance productivity of gal80 mutant, an alternative strain, allgal, was developed by removing all galactose-utilizing genes that consume significant cellular resources in the gal80 strain when cultured in non-galactose conditions. The efficacy of the allgal mutant (gal80, gal1, gal2, gal7, and gal10) was verified by assessing the secretory expression of three recombinant proteins, Candida antarctica lipase B (CalB), human serum albumin (HSA), and human epidermal growth factor (hEGF), using the GAL10 promoter. The growth of the allgal mutant was enhanced by 15–38% compared to the gal80 mutant, and the secretion of recombinant proteins also increased by 16–22% in fed-batch fermentation. Thus, the expression of recombinant proteins using GAL10 promoter in the allgal mutant is suitable for the economical production of recombinant proteins in S. cerevisiae.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"36 ","pages":"Article e00763"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/82/main.PMC9493058.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33485053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Control of carbendazim toxicity using banana peel powder in rats 香蕉皮粉对大鼠多菌灵毒性的控制

Biotechnology Reports Pub Date : 2022-12-01 DOI: 10.1016/j.btre.2022.e00773

Gomaa N. Abdel-Rahman , Ahmed S.M. Fouzy , May M. Amer , Essam M. Saleh , Islam A. Hamed , Bassem A. Sabry

引用次数: 2