Antioxidant and antiproliferative activity of enzymatic hydrolysates from red tilapia (Oreochromis spp.) viscera

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2024-02-28 DOI:10.1016/j.btre.2024.e00832

José E. Zapata , Leidy J. Gómez-Sampedro

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引用次数: 0

Abstract

The antioxidant and antiproliferative activity of red tilapia (Oreochromis spp.) viscera hydrolysates (RTVH) was evaluated. For that, the hydrolysates was applied to three cancer cell lines (HepG2, Huh7 and SW480) and the control (CCD-18Co). Finally, the line on which the hydrolysate had the greatest effect (SW480) and the control (CCD-18Co) were subjected to the ApoTox-Glo Triplex Assay to determine apoptosis, toxicity, and cell viability. The result showed that hydrolysate had a dose-dependent cytotoxic effect selective on the three cancer cell lines, compared to the control cells. There is a relationship between the antioxidant capacity of RTVHs and their antiproliferative capacity on cancer cells evaluated, which achieved cell viability by action of RTVH of 34.68 and 41.58 and 25.41 %, to HepG2, Huh7 and SW480, respectively. The action of RTVH on cancer cell line SW480 is not due to the induction of apoptosis but to the rupture of the cell membrane.

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红罗非鱼（Oreochromis spp.）内脏酶水解物的抗氧化和抗增殖活性

研究评估了红罗非鱼（Oreochromis spp.）内脏水解物（RTVH）的抗氧化和抗增殖活性。为此，将水解物用于三种癌细胞系（HepG2、Huh7 和 SW480）和对照组（CCD-18Co）。最后，对水解物影响最大的细胞系（SW480）和对照组（CCD-18Co）进行了 ApoTox-Glo Triplex 分析，以确定细胞凋亡、毒性和细胞活力。结果表明，与对照细胞相比，水解物对三种癌细胞株具有剂量依赖性的选择性细胞毒性作用。RTVH 的抗氧化能力与其对癌细胞的抗增殖能力之间存在一定的关系，在 RTVH 的作用下，HepG2、Huh7 和 SW480 的细胞存活率分别为 34.68%、41.58% 和 25.41%。RTVH 对癌细胞 SW480 的作用不是由于诱导细胞凋亡，而是由于细胞膜破裂。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.