Biotechnology Reports最新文献

{"title":"Valorization of agricultural residues for bioplastic production by bacteria isolated from plastic dumpsites: Integrating waste streams into the circular bioeconomy","authors":"Olawale Sunday Olayiwola ,&nbsp;Oladipo Oladiti Olaniyi ,&nbsp;Elizabeth Odunmbaku ,&nbsp;Temitope Ojuolape Fadipe ,&nbsp;Grace Odunayo Oyinloye ,&nbsp;Ridwan Ayomide Amoo ,&nbsp;Tolulope A. Odeshi","doi":"10.1016/j.btre.2025.e00941","DOIUrl":"10.1016/j.btre.2025.e00941","url":null,"abstract":"<div><div>Polyhydroxybutyrate (PHB) is a biodegradable polymer produced by bacteria under nutrient-limiting conditions, offering a sustainable alternative to petroleum plastics. This study investigated PHB production by bacterial isolates from plastic-contaminated soils. Forty isolates were screened using Sudan Black B, Nile Blue A, and Nile Red; 12 tested positive, and 11 promising strains belonging to genera, <em>Corynebacterium, Bacillus, Micrococcus, Arthrobacter</em>, and <em>Sinomonas</em> were selected for detailed study. Cultivation in nutrient-limiting medium with 2% sugars (fructose, glucose, sucrose, galactose, mannitol) or agro-wastes (cassava wastewater, cassava and potato peels) revealed strain- and substrate-dependent PHB accumulation, peaking at 24–96 h. <em>Micrococcus</em> sp<em>.</em> OO(14)-5 produced the highest PHB content (0.15 g/L) on mannitol, while <em>Corynebacterium</em> sp. FT(1)-6 and <em>Bacillus</em> sp. GO(10)-6 yielded 3.2–3.4 g/L and ∼60% PHB/CDW on cassava wastewater. Other isolates produced &gt;2.7 g/L on peels. Fourier-transform infrared spectroscopy confirmed PHB. These findings highlight low-cost agro-wastes as effective substrates for sustainable PHB production.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00941"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenesis and advances in immunotherapy for type 1 diabetes treatment","authors":"Tran Chi Linh ,&nbsp;Nguyen Khanh Dung","doi":"10.1016/j.btre.2025.e00940","DOIUrl":"10.1016/j.btre.2025.e00940","url":null,"abstract":"<div><div>Type 1 diabetes (T1D) is an autoimmune disease marked by selective destruction of pancreatic β-cells, resulting in absolute insulin deficiency. Although insulin replacement remains the standard therapy, it does not address the underlying autoimmune process or prevent long-term complications. Advances in understanding the pathogenesis have highlighted the interaction of genetic susceptibility, environmental triggers, and immune dysregulation, paving the way for innovative immunotherapies. Current strategies include nonspecific immunosuppressants, monoclonal antibodies (e.g., teplizumab, rituximab), peptide vaccines, and cell-based therapies such as regulatory T cells and stem cells. Among these, teplizumab has gained FDA approval to delay disease onset in high-risk individuals, representing a milestone in preventive intervention. Nevertheless, limited durability, high costs, and safety concerns restrict broader clinical application. Looking forward, personalized treatment strategies, rational drug combinations, and early preclinical interventions are expected to optimize outcomes, offering new hope for improving prognosis and quality of life in T1D patients.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00940"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert","authors":"Seyed Saeed Sekhavatizadeh ,&nbsp;Neda Maftoonazad ,&nbsp;Hadis Motashafi ,&nbsp;Mehdi Nikkhah ,&nbsp;Maryam Asnaashari","doi":"10.1016/j.btre.2026.e00950","DOIUrl":"10.1016/j.btre.2026.e00950","url":null,"abstract":"<div><div>This study is the first to utilize nano-scale Qodume Shirazi seed mucilage (QSSM) and <em>Camelina sativa</em> seed mucilage (CSSM), individually and in combination, as natural wall materials for microencapsulation of <em>Lacticaseibacillus rhamnosus</em> (LR). SEM analysis indicated smooth bead surfaces and successful entrapment of LR. The 50:50 QSSM: CSSM formulation exhibited 97.92% encapsulation efficiency, and microencapsulated LR (MLR) remained viable (&gt;6 log CFU/g) after 15 min at 72 °C. Moreover, MLR maintained viability above 7.85 log CFU/mL for 21 days at 4 °C in goat milk dessert (GMD). Under simulated gastrointestinal conditions MLR demonstrated a 57.53% survival rate compared with free LR, which was 47.17% at the end of storage. The addition of MLR increased the hardness (258.0 ± 16.0 g) and gumminess (102.56 ± 8.45 g) of GMD. Overall, the results demonstrate that QSSM and CSSM mucilages can serve as effective natural encapsulating agents, enhancing the thermal and storage stability.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00950"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147310733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus altitudinis GG-22: A novel plant growth-promoting bacterium with beneficial agronomic properties","authors":"Ana Falcón-Piñeiro ,&nbsp;Alberto Baños ,&nbsp;Eva M. Molin ,&nbsp;Elías González-Gragera ,&nbsp;Annalisa Giampetruzzi ,&nbsp;Raied Abou Kubaa ,&nbsp;Livio Antonielli ,&nbsp;Adrian Wallner ,&nbsp;Günter Brader ,&nbsp;Satish K. Verma ,&nbsp;Antonio M. Martin-Platero ,&nbsp;Manuel Martínez-Bueno ,&nbsp;Stéphane Compant ,&nbsp;Pasquale Saldarelli","doi":"10.1016/j.btre.2026.e00945","DOIUrl":"10.1016/j.btre.2026.e00945","url":null,"abstract":"<div><div><em>Bacillus altitudinis</em> GG-22, isolated from the phyllosphere of agricultural crops, has been identified as a promising biocontrol agent and plant growth-promoting bacterium with substantial potential in sustainable agriculture. In this study, whole-genome sequencing using Illumina technology, combined with ANI analysis, confirmed the strain's classification as <em>B. altitudinis</em>. The genome revealed a rich set of genes involved in biocontrol mechanisms, including the capacity of synthesis of siderophores (schizokinen and bacillibactin-like compounds), the lipopeptide pumilacidin, the bacteriocin pumilarin, alkylpyrones and <em>Bacillus</em> volatiles. <em>In vitro</em> antagonism assays demonstrated significant inhibitory effects against phytopathogenic fungi and oomycetes, such as <em>Verticillium dahliae</em> and <em>Pythium</em> sp., and <em>B. altitudinis</em> GG-22 also showed limited efficacy against bacterial phytopathogens, including <em>Xylella fastidiosa</em>. Transcriptomic profiling of olive trees treated with GG-22 indicated early activation of auxin transport and systemic acquired resistance (SAR) pathways, alongside substantial downregulation of cell wall remodelling genes. These findings suggest that <em>B. altitudinis</em> GG-22 primes plant defence responses and modulates hormonal pathways critical for growth and stress resilience. Future research should prioritize optimizing application strategies and exploring synergies with other microbial agents to fully harness the biocontrol and growth-promoting potential of <em>B. altitudinis</em> GG-22. This strain holds promise for sustainable agricultural practices, particularly in controlling fungal diseases and improving plant performance under stress conditions.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00945"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring differential gene correlations in Arabidopsis thaliana for sustainable space farming","authors":"Federica D’Annunzio ,&nbsp;Giulia Fiscon ,&nbsp;Federica Conte ,&nbsp;Paola Paci ,&nbsp;Marta Del Bianco ,&nbsp;Raffaele Dello Ioio","doi":"10.1016/j.btre.2026.e00949","DOIUrl":"10.1016/j.btre.2026.e00949","url":null,"abstract":"<div><div>The plans for sustained human presence on the Moon have increased interest in understanding the effects of lunar conditions on terrestrial biology. Plants play a vital role in space exploration, as they not only generate oxygen and remove carbon dioxide but also provide fresh food that can be rich in nutraceutical compounds important for astronaut survival in long-duration missions. Understanding how to leverage in-situ resources, such as lunar regolith, is crucial for sustainable space exploration. However, using regolith as substrate for plant growth is challenging due to its composition and lack of organic matter. While previous research has primarily examined the gene expression of <em>Arabidopsis thaliana</em> under lunar conditions, it has mainly focused on individual gene activity, neglecting the interactions between genes within networks. This study goes beyond traditional approaches by employing differential gene correlation analysis to explore how gene pairs interact across different regolith environments. This strategy provides a deeper understanding of plant adaptation mechanisms, offering valuable insights for optimizing plant growth in extraterrestrial environments.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00949"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neural Latent Filtering for Gene Discovery in Breast Cancer Subtypes","authors":"Danilo Menegatti ,&nbsp;Giulia Fiscon ,&nbsp;Alessandro Giuseppi ,&nbsp;Paola Paci ,&nbsp;Antonio Pietrabissa","doi":"10.1016/j.btre.2025.e00942","DOIUrl":"10.1016/j.btre.2025.e00942","url":null,"abstract":"<div><div>Gene selection from expression data represents a challenging task, primarily due to the high data dimensionality and the vast number of genes that would be identified, many of which may be unrelated to cancer-relevant biological processes. To tackle this issue, filtering methods constitute an effective solution to identify the most informative genes, which can serve as potential biomarkers to tailor cancer therapies. This work proposes a novel neural-based filtering approach which aims to identify genes by means of their latent representation extracted from RNA Sequencing expression data. This approach has been applied to study breast invasive carcinoma dataset, aiming to identify the most relevant genes of two breast cancer subtypes, Luminal-A and Basal-like, to better investigate their molecular landscape.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00942"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EIOFX-DT: Leveraging graph centrality metrics for feature extraction and classification of viral genetic sequences","authors":"Amin Khodaei ,&nbsp;Zahra Pourabbas ,&nbsp;Fatemeh Hashem-zadehdizajyekan ,&nbsp;Erfan Esmaeili","doi":"10.1016/j.btre.2025.e00939","DOIUrl":"10.1016/j.btre.2025.e00939","url":null,"abstract":"<div><div>Many diseases have a genetic origin, and analyzing intracellular structures through genetic data yields specific features for the diagnosis and classification of viral disease samples. In this study, 30 types of viruses were analyzed using a graph-based approach on genetic data. Genetic data has been modeled in the form of genomic sequences at the nucleotide scale using the graph theory of complex networks concepts. Degree and eigenvector centrality metrics were employed to extract features. The decision tree was utilized as a machine learning classifier algorithm on the resulting feature space. The results, presented in the form of interpretable rules, enable the classification and identification of virus types from both a binary and multi-class perspective. The model achieved high accuracy and f1 score, which exceeded 99 % on &gt;173,000 samples. Additionally, the feature extraction algorithm demonstrated robust performance across all datasets and classifiers.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00939"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and characterization of rNGFSP: a recombinant fusion immunogen eliciting dual anti-NGF and anti-Substance P therapeutic antibodies for Degenerative Joint Disease","authors":"Valentina Varela ,&nbsp;Monique Costa ,&nbsp;Cecilia Maciel ,&nbsp;Joaquín Barbeito ,&nbsp;Exequiel E. Barrera ,&nbsp;Erica Gutierre ,&nbsp;Agustín Correa ,&nbsp;Melania Elgue ,&nbsp;Sebastián Carrasco ,&nbsp;Magdalena Domínguez Larrosa ,&nbsp;María Pereira ,&nbsp;Josefina Correa ,&nbsp;Nadia Crosignani ,&nbsp;Joseph S. Beckman ,&nbsp;Luis Barbeito ,&nbsp;Emiliano Trias","doi":"10.1016/j.btre.2026.e00946","DOIUrl":"10.1016/j.btre.2026.e00946","url":null,"abstract":"<div><div>Anti-NGF monoclonal antibodies have recently been approved for treating degenerative joint disease, including osteoarthritis pain, in dogs and cats. However, their widespread use is limited by high cost and the requirement for repeated injections. Nerve Growth Factor and Substance P play central roles in the initiation and maintenance of inflammation and chronic pain in OA. There is a pressing need for new, safe, cost-effective therapies that target the underlying mechanisms of OA chronic pain. Here, we designed and produced a novel recombinant fusion protein, termed rNGFSP, which functions as an immunogen due to its unique molecular structure combining amino acid sequences from NGF and SP in a non-native conformation. When formulated and administered as a vaccine, rNGFSP elicits dual anti-NGF and anti-SP therapeutic antibodies in the host. rNGFSP was produced in <em>E. coli</em> and purified from inclusion bodies using metal affinity chromatography under denaturing conditions. Mass spectrometry confirmed the expected molecular weight (17.5 kDa) and preserved amino acid sequence. Structural prediction using Alphafold2 revealed rNGFSP presented a non-natural folding, but a preserved NGF core and a flexible SP tail, supporting antigenic presentation. Vaccination of mice, rabbits, horses, and dogs, showed that rNGFSP elicited cross-reactive IgG antibodies against the native conformations of NGF and SP. Furthermore, immunoglobulins elicited in vaccinated dogs neutralized the biological activity of NGF and SP in cell cultures, suggesting a therapeutic potential. These findings support rNGFSP as a promising vaccine candidate simultaneously targeting endogenous NGF and SP species, providing a cost-effective alternative to monoclonal antibodies.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00946"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and characterization of a HEK293 cell line optimized for recombinant adeno-associated virus production","authors":"Mélissa Vona ,&nbsp;Iris Bodenmann ,&nbsp;Marc-Antoine Perrenoud,&nbsp;Rachel Buchs,&nbsp;Pelin Kolcak Yasli,&nbsp;Luca Nanni,&nbsp;Romain Daveau,&nbsp;Alexandre Félix,&nbsp;Jens Stolte,&nbsp;Ann-Kristin Hov,&nbsp;Bertrand Chollet,&nbsp;Thierry Schuepbach,&nbsp;Déborah Ley,&nbsp;Efrain Guzman,&nbsp;Igor Fisch,&nbsp;Nicolas Mermod","doi":"10.1016/j.btre.2026.e00948","DOIUrl":"10.1016/j.btre.2026.e00948","url":null,"abstract":"<div><div>HEK293 is a preferred cellular platform to produce viral vectors including adeno-associated viruses (AAV). However, HEK293 cells were shown to be genomically unstable and many HEK293 cell lines having distinct genotypes and phenotypes have been reported. Here we generated a stable clonal cell line specifically selected for the optimal production of recombinant AAV (rAAV) by the triple plasmid transfection method. Initially over two thousand single cell clones were isolated from a HEK293 polyclonal cell line and evaluated for their growth profile in suspension, doubling time, ability to recover freeze-thaw cycles and transfection efficacy. A selection of clones that met these specific criteria were then screened for their ability to produce high rAAV titers by triple plasmid transfection, yielding one high-performing clone named NBX1P01. This clone was genomically characterized using optical genome mapping and whole genome sequencing and further evaluated for rAAV production capacity across different serotypes and genes of interest (GOI). NBX1P01 was shown to be genomically stable over 55 population doubling levels (PDL), highly transfectable and able to produce rAAV titers similar or higher than those produced by a commercially available HEK293 cell line using the same culture, transfection, harvest and quantification protocol. The ratio of full-to-empty rAAV particles produced by NBX1P01 was two-fold higher than those of the commercial cell line. Long-read sequencing of the encapsidated DNA from the NBX1P01-produced rAAV indicated high levels of genome integrity with minimal levels of contaminants. These results demonstrated the versatility of NBX1P01 cells and their ability to produce high-quality rAAV vectors.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00948"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of anti-dengue virus D8 neutralizing monoclonal antibody production in Nicotiana benthamiana","authors":"Gunt Niyompun ,&nbsp;Kaewta Rattanapisit ,&nbsp;Janejira Jaratsittisin ,&nbsp;Pipob Suwanchaikasem ,&nbsp;Rochanawan Sootichote ,&nbsp;Pichamon Sittikul ,&nbsp;Pannamthip Pitaksajjakul ,&nbsp;Pongrama Ramasoota ,&nbsp;Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2026.e00951","DOIUrl":"10.1016/j.btre.2026.e00951","url":null,"abstract":"<div><div>Dengue is a life-threatening mosquito-borne viral disease ranging from mild symptoms to severe hemorrhagic fever. In this study, a recombinant anti-dengue D8 monoclonal antibody was produced in <em>Nicotiana benthamiana</em> and characterized for protein integrity and glycosylation by LC-MS. The plant-derived antibody lacked plant-specific β1,2-xylose and core α1,3-fucose residues, displaying a mammalian-like glycan profile. Functional evaluation showed potent cross-neutralizing activity against all four dengue virus serotypes (DENV1–DENV4), with the strongest activity against DENV4 (FRNT50 &lt; 1 µg/mL), followed by DENV2 (5.82 µg/mL), DENV3 (9.49 µg/mL), and DENV1 (28.68 µg/mL), comparable to the mammalian-produced counterpart. The antibody also bound strongly to NS1 proteins of all serotypes, especially DENV2, and demonstrated higher reactivity than mammalian-derived anti-NS1 antibodies. Collectively, our results provide proof-of-concept that a glycoengineered plant platform can generate a functional D8 antibody with mammalian-like glycosylation, robust NS1 binding, and cross-neutralizing activity against DENV1–4, prompting further evaluation in Fc-dependent assays and in vivo models.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"49 ","pages":"Article e00951"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147327584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}