Biotechnology Reports最新文献

Prioritizing repurposable drugs for Alzheimer’s disease using network-based analysis with concurrent assessment of Long QT syndrome risk 利用基于网络的分析对阿尔茨海默病的可重复使用药物进行优先排序，同时评估长QT综合征的风险

Biotechnology Reports Pub Date : 2025-07-29 DOI: 10.1016/j.btre.2025.e00909

Alessio Funari , Enrico De Smaele , Paola Paci , Giulia Fiscon

{"title":"Prioritizing repurposable drugs for Alzheimer’s disease using network-based analysis with concurrent assessment of Long QT syndrome risk","authors":"Alessio Funari , Enrico De Smaele , Paola Paci , Giulia Fiscon","doi":"10.1016/j.btre.2025.e00909","DOIUrl":"10.1016/j.btre.2025.e00909","url":null,"abstract":"<div><div>Alzheimer's disease affects 6.9 million Americans aged 65 and older, a number expected to double by 2060. Eight FDA-approved drugs target Alzheimer's, but no cure is available, and most treatments are symptomatic. Drug repurposing, the use of FDA-approved drugs for new indications, is a promising strategy to address this lack of effective therapies. However, despite prior safety approval, repurposable drugs may still trigger unexpected side-effects in new contexts. This study introduces a network-based approach to minimize side-effect risk in drug repositioning, focusing on QT interval prolongation, a cardiac side-effect observed in Alzheimer's patients treated with acetylcholinesterase inhibitors. The method integrates Mode-of-Action and Random Walk with Restart analyses to identify repositioning candidates while assessing QT-related risk. This strategy identified promising compounds including acamprosate, tolcapone, sitagliptin, and diazoxide, with potential to mitigate disease pathology. Gene set enrichment analysis was used to computationally assess the compounds' ability to reverse disease-related gene expression signatures.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00909"},"PeriodicalIF":0.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing cell characterization with microfluidics and AI: a comprehensive review of mechanical, electrical, and hybrid techniques 用微流体和人工智能增强细胞表征：机械、电气和混合技术的综合综述

Biotechnology Reports Pub Date : 2025-07-22 DOI: 10.1016/j.btre.2025.e00905

Marwan Taha , Anna-Maria Pappa , Hani Saleh , Anas Alazzam

引用次数: 0

Methylene Blue Removal Using Alginate-PVA-Pseudomonas aeruginosa Biocomposite: Kinetics and Biodegradation Studies 海藻酸盐- pva -铜绿假单胞菌生物复合材料去除亚甲基蓝：动力学和生物降解研究

Biotechnology Reports Pub Date : 2025-07-17 DOI: 10.1016/j.btre.2025.e00906

Silvia Abdi Pratama , Adi Setyo Purnomo , Asranudin Asranudin

{"title":"Methylene Blue Removal Using Alginate-PVA-Pseudomonas aeruginosa Biocomposite: Kinetics and Biodegradation Studies","authors":"Silvia Abdi Pratama , Adi Setyo Purnomo , Asranudin Asranudin","doi":"10.1016/j.btre.2025.e00906","DOIUrl":"10.1016/j.btre.2025.e00906","url":null,"abstract":"<div><div>Methylene blue (MB) is a common synthetic dye used in various industries due to its abundance and cost-effectiveness. However, the presence of this synthetic dye in industrial wastewater has the potential to cause significant effects on the environment and public health when released into soil or water bodies. According to results, biological wastewater treatment has emerged as a promising approach due to its economic feasibility, efficiency, and environmental compatibility. Therefore, this study aims to evaluate the MB removal by <em>Pseudomonas aeruginosa</em> immobilized within alginate–polyvinyl alcohol (PVA) matrices and compare removal performance to that of free (non-immobilized) cells. The results showed that the immobilized <em>P. aeruginosa</em> (Alg-PVA-PA beads) exhibited higher MB removal efficiency (72.52%) compared to free cells (55.52%) and Alg-PVA beads alone (43.12%). Adsorption analysis showed that the removal process followed a pseudo-second-order kinetic model, suggesting chemisorption, and was best described by the Langmuir isotherm, showing monolayer adsorption. Additionally, reusability tests showed that the immobilized biocomposite beads retained over 35% MB removal efficiency after five consecutive cycles, suggesting the sustainability of the beads. XRD, FTIR, and SEM-EDX analysis confirmed the successful entrapment of <em>P. aeruginosa</em> within the Alg-PVA matrices and showed structural changes in the beads following the MB removal process. The results were also supported by LC-QTOF/MS outcomes, which showed metabolites with retention times of 1.17, 1.52, 8.35, and 9.36 min, suggesting the successful degradation of MB.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00906"},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of the duckweed biomass and starch production utilizing biogenic MnO and ZnO microparticles 利用生物源MnO和ZnO微颗粒提高浮萍生物量和淀粉产量

Biotechnology Reports Pub Date : 2025-07-16 DOI: 10.1016/j.btre.2025.e00907

Maryam Anar , Sajjad Kamal Shuvro , Muhammad Farooq Hussain Munis , Masaaki Morikawa

引用次数: 0

Investigation and optimization of DNA isolation efficiency using ferrite-based magnetic nanoparticles 铁氧体基磁性纳米颗粒DNA分离效率的研究与优化

Biotechnology Reports Pub Date : 2025-06-30 DOI: 10.1016/j.btre.2025.e00904

Tímea B. Gerzsenyi , Ágnes M. Ilosvai , Ferenc Kristály , Lajos Daróczi , Michael C. Owen , Béla Viskolcz , László Vanyorek , Emma Szőri-Dorogházi

{"title":"Investigation and optimization of DNA isolation efficiency using ferrite-based magnetic nanoparticles","authors":"Tímea B. Gerzsenyi , Ágnes M. Ilosvai , Ferenc Kristály , Lajos Daróczi , Michael C. Owen , Béla Viskolcz , László Vanyorek , Emma Szőri-Dorogházi","doi":"10.1016/j.btre.2025.e00904","DOIUrl":"10.1016/j.btre.2025.e00904","url":null,"abstract":"<div><div>DNA isolation is a crucial step in many molecular biological applications for diagnostic and research purposes, like detection of infectious diseases or gene expression studies. However, due to the requirement of toxic reagents in traditional procedures and the high expenses of commercial kits, the use of magnetic MNP-based DNA isolation is becoming more widespread. In this study, different ferrite containing MNPs (MnFe<sub>2</sub>O<sub>4</sub>, MnFe<sub>2</sub>O<sub>4</sub>-NH<sub>2</sub>, MgFe<sub>2</sub>O<sub>4</sub>, MgFe<sub>2</sub>O<sub>4</sub>-NH<sub>2</sub> NiFe<sub>2</sub>O<sub>4</sub>, NiFe<sub>2</sub>O<sub>4</sub>-NH<sub>2</sub>) are examined and compared in their pDNA isolation efficiency. Among the tested nanoparticles, we document the use of NiFe<sub>2</sub>O<sub>4</sub> and its amine-functionalized form for the first time. Three protocols for the isolation of pDNA are optimized for each type of nanoparticle and the best protocol is selected based on the quantity, quality and integrity of the extracted DNA. Plasmid samples extracted with the MNPs are transformed into competent bacterial cells and further tests are performed to recover genomic DNA from bacterial cells, leading to the development of another protocol. Bacteria-spiked blood serum samples are produced to extract DNA from a more complex biological matrix.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00904"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative analysis of RAS signaling effectors reveals stage-dependent oncogenic patterns in colon adenocarcinoma RAS信号效应的综合分析揭示了结肠腺癌的分期依赖性致癌模式

Biotechnology Reports Pub Date : 2025-06-23 DOI: 10.1016/j.btre.2025.e00902

Loretta László , Anna Lovrics , Álmos Tilajka , Tamás Takács , László Buday , Virag Vas

{"title":"Integrative analysis of RAS signaling effectors reveals stage-dependent oncogenic patterns in colon adenocarcinoma","authors":"Loretta László , Anna Lovrics , Álmos Tilajka , Tamás Takács , László Buday , Virag Vas","doi":"10.1016/j.btre.2025.e00902","DOIUrl":"10.1016/j.btre.2025.e00902","url":null,"abstract":"<div><div>Cancer rarely results from a single gene defect but emerges from disruptions in complex cellular networks. The Network Medicine perspective guides our investigation of cancer-driving interactions, particularly focusing on RAS signaling pathways that are key mediator for cancer development.</div><div>We analyzed gene expression patterns in colon and lung cancers to identify stage-specific molecular drivers. Using computational modelling combined with patient tissue analysis, we discovered five key genes that are specifically altered in early-stage colon cancer: RAF1, PLCE1, RGL1, RIN1, and GRB7. These genes work as RAS effectors in signaling and can effectively distinguish between normal and cancerous colon tissue.</div><div>Our approach combines network analysis with gene expression studies to understand how RAS signaling disruption contributes to colon cancer development. These findings suggest that targeting early-stage RAS-related changes could offer therapeutic opportunities before cancer becomes more complex and harder to treat.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00902"},"PeriodicalIF":0.0,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scale-down optimization of a robust, parallelizable human induced pluripotent stem cell bioprocess for high-throughput research 用于高通量研究的稳健、可并行的人诱导多能干细胞生物过程的缩小优化

Biotechnology Reports Pub Date : 2025-05-22 DOI: 10.1016/j.btre.2025.e00900

James Colter , Tiffany Dang , Julia Malinovska , Jessica May Corpuz , Dora Modrcin , Roman Krawetz , Kartikeya Murari , Michael Scott Kallos

{"title":"Scale-down optimization of a robust, parallelizable human induced pluripotent stem cell bioprocess for high-throughput research","authors":"James Colter , Tiffany Dang , Julia Malinovska , Jessica May Corpuz , Dora Modrcin , Roman Krawetz , Kartikeya Murari , Michael Scott Kallos","doi":"10.1016/j.btre.2025.e00900","DOIUrl":"10.1016/j.btre.2025.e00900","url":null,"abstract":"<div><div>Human induced pluripotent stem cell (hiPSC) derived therapeutics require clinically relevant quantities of high-quality cell populations for applications in regenerative medicine. The lack of efficacy exhibited across clinical trials suggests deeper understanding of the networks governing phenotype is needed. Further, costs limit study throughput in characterizing the artificial niche relative to outcomes. We present herein an optimized strategy to enable high-throughput hiPSC expansion at <20 mL research scale. We assessed viability of single cell inoculation and aggregate preformation to facilitate proliferation. We modeled aggregate characteristics against agitation rate. Our results demonstrate tunable control with fold expansion comparable to commercial systems. Marker quantification and teratoma assay confirm functional pluripotency. This approach constitutes a scalable protocol to accelerate hiPSC research, and a significant step in advancing the rate of progress in elucidating links to derivative functionality. This work will enable statistically rigorous studies targeting hiPSC and downstream phenotype for clinical manufacturing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00900"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation of Cupriavidus necatorstrains on hydrolyzed lignocellulosic feedstocks widely available in Europe 在欧洲广泛使用的水解木质纤维素原料上培养番茄铜球菌菌株

Biotechnology Reports Pub Date : 2025-05-20 DOI: 10.1016/j.btre.2025.e00899

Halima Aliyu Alhafiz , Karin Longus , Rob A.J. Verlinden , Vera Lambauer , Andreas Kruschitz , Regina Kratzer

引用次数: 0

Induction conditions that promote the effect of glycerol on recombinant protein production in Escherichia coli 促进甘油对大肠杆菌重组蛋白产生影响的诱导条件

Biotechnology Reports Pub Date : 2025-05-16 DOI: 10.1016/j.btre.2025.e00898

Yoshihiro Ojima , Hajime Saito , Shintaro Miyuki , Koichi Fukunaga , Terumichi Tsuboi , Masayuki Azuma

引用次数: 0

Production and evaluation of rabies immunoglobulin extracted from chicken egg yolk 鸡蛋黄狂犬病免疫球蛋白的制备及评价

Biotechnology Reports Pub Date : 2025-05-08 DOI: 10.1016/j.btre.2025.e00897

Vichununt Kerdput , Vararut Yodkamol , Mattika Sookprasong , Wongsakorn Wongwadhunyoo , Iyacoob Khunsri , Promsin Masrinoul , Trong Wisedchanwet , Naphatsamon Uthailak , Yanin Limpanont , Onrapak Reamtong , Poom Adisakwattana , Charin Thawornkuno

{"title":"Production and evaluation of rabies immunoglobulin extracted from chicken egg yolk","authors":"Vichununt Kerdput , Vararut Yodkamol , Mattika Sookprasong , Wongsakorn Wongwadhunyoo , Iyacoob Khunsri , Promsin Masrinoul , Trong Wisedchanwet , Naphatsamon Uthailak , Yanin Limpanont , Onrapak Reamtong , Poom Adisakwattana , Charin Thawornkuno","doi":"10.1016/j.btre.2025.e00897","DOIUrl":"10.1016/j.btre.2025.e00897","url":null,"abstract":"<div><div>This study investigated the potential of using laying hens to produce rabies immunoglobulin (RIG) due to concerns about an African Horse Sickness outbreak in 2020. The hens were immunized with rabies vaccine, resulting in the production of Immunoglobulin Y (IgY) antibodies in their egg yolks. The study found that using polyethylene glycol 6000 (PEG) was the most effective method for extracting anti-rabies IgY from egg yolks in terms of amount (1.87 mg of IgY from one mL of egg yolk), purity (83 %), and cost-effectiveness. Further purification through affinity chromatography increased the purity to 95 %, as confirmed by 1D GeLC-MS/MS, showing a purity of up to 98 %. In addition, IgY extracted using PEG demonstrated the highest neutralizing activity compared to the caprylic acid and ammonium sulfate methods. It was estimated that only 8 eggs are needed to produce a vial of RIG with the same potency as equine RIG (1000 IU/vial).</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00897"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0