Biotechnology Reports最新文献

{"title":"Sustainable fuel and power from biomass: 4E analysis of a solar-assisted DME production system with CO₂ capture","authors":"Atieh Kermani ,&nbsp;Farzin Hosseinifard ,&nbsp;Mohsen Salimi ,&nbsp;Majid Amidpour","doi":"10.1016/j.btre.2025.e00928","DOIUrl":"10.1016/j.btre.2025.e00928","url":null,"abstract":"<div><div>Transitioning to clean energy requires efficient, low-carbon fuel production methods. Traditional biomass-to-fuel approaches are limited by inefficiency, cost, and emissions. This study presents an innovative system based on renewable lignocellulosic biomass to produce dimethyl ether (DME), methanol, and electricity simultaneously. The design integrates thermochemical conversion, solar thermal energy, internal power generation, and post-combustion CO₂ capture. Waste heat and solar energy drive dual electricity-producing loops, enabling internal energy sufficiency and surplus sale. Simulations indicate 50 % total energy efficiency and 49 % exergy efficiency, with hourly production of 2.7 tons DME and 0.56 tons methanol. Economic analysis shows baseline feasibility with an NPV of ∼$530 M and payback period ∼5.5 years; sensitivity to biomass price, capital cost, and discount rate is noted, highlighting potential uncertainty ranges. This integrated pathway offers a scalable, low-carbon, and economically viable solution for sustainable bioenergy.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00928"},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycoengineering of plant-produced Pembrolizumab enhances FcRn binding and extends serum half-life in mice","authors":"Christine Joy I. Bulaon ,&nbsp;Janejira Jaratsittisin ,&nbsp;Kaewta Rattanapisit ,&nbsp;Pipob Suwanchaikasem ,&nbsp;Shiying Guo ,&nbsp;Khwanchit Boonha ,&nbsp;Pannamthip Pitaksajjakul ,&nbsp;Nipaporn Simsom ,&nbsp;Vudhiporn Limprasutr ,&nbsp;Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2025.e00927","DOIUrl":"10.1016/j.btre.2025.e00927","url":null,"abstract":"<div><div>Plant systems offer scalable and cost-effective platforms for antibody production, but plant-specific glycans may affect pharmacokinetics and immunogenicity. To evaluate the impact of Fc glycosylation, four Pembrolizumab glycovariants were generated in <em>Nicotiana benthamiana</em>: wild-type glycosylation (Pembro-WT), high-mannose with SEKDEL (Pembro-KD), aglycosylated N297A mutant (Pembro-NG), and a core fucose/xylose-deficient variant (Pembro-XF). Glycoproteins were transiently expressed either in wild-type or ΔXF plants, purified, and characterized for glycan composition, in vitro binding, and in vivo pharmacokinetics. LC-MS confirmed distinct glycoform patterns, while PD-1 binding was retained across all variants. Pembro-XF showed the highest FcRn binding affinity and longest serum half-life (45.83 h) in mice, compared to Pembro-WT (26.7 h), Pembro-KD (32.95 h), Pembro-NG (34.27 h), and Keytruda® (33.26 h). As an initial efficacy evaluation, Pembro-WT demonstrated strong antitumor activity in a murine colon cancer model. These findings support plant glycoengineering as a strategy to enhance antibody pharmacokinetics and advance next generation antibody therapeutics.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00927"},"PeriodicalIF":0.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery and engineering of bifunctional enzymes for lignocellulose degradation: Metagenomic and computational approaches","authors":"Razieh Goudarzi ,&nbsp;Donya Afshar Jahanshahi ,&nbsp;Arashk Kavousi ,&nbsp;Shohreh Ariaeenejad","doi":"10.1016/j.btre.2025.e00926","DOIUrl":"10.1016/j.btre.2025.e00926","url":null,"abstract":"<div><div>Efficient degradation of lignocellulosic biomass is vital for converting plant-based waste into renewable fuels and chemicals. Owing to its complex composition of cellulose, hemicellulose, and lignin, its enzymatic breakdown often requires multiple enzymes to act synergistically. Bifunctional enzymes that combine two catalytic activities in a single protein offer a promising solution. This review highlights recent advances in the identification and engineering of bifunctional enzymes for lignocellulose degradation, particularly through metagenomics, protein fusion and computational design. Functional pairings, such as cellulase/xylanase, were examined with a focus on their synergistic effects, substrate specificity, and stability. Promiscuous and naturally evolved bifunctional enzymes from extreme or uncultured environments are also discussed. Advances <em>in silico</em> modeling and directed evolution have enhanced enzyme properties such as thermostability and substrate range. The review concludes with an outlook on the challenges and opportunities of implementing bifunctional enzymes to improve the economic and technical viability of biomass conversion.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00926"},"PeriodicalIF":0.0,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical modelling and experimental validation of an orifice plate-based hydrodynamic cavitation process for improving the biomass pretreatment","authors":"Shetal Roy ,&nbsp;Souman Rudra ,&nbsp;DI Muhandiram ,&nbsp;MM Roshid","doi":"10.1016/j.btre.2025.e00925","DOIUrl":"10.1016/j.btre.2025.e00925","url":null,"abstract":"<div><div>Hydrodynamic cavitation can be used to pretreat biomass by utilizing the energy released during the collapse of cavitation bubbles. Delignification as pretreatment enhances the biodegradation of lignocellulosic composition. The present study employed a CFD model and experimental validation of the numerical analysis of hydrodynamic cavitation (HC) in multi-hole orifice (MHO) designs under various operating conditions. The total 14 geometry of the orifice plate depends on the plate thickness, the number of holes, and the hole orientation used to analyze and optimize geometry for the biomass pretreatment process. Three phases are used in numerical analysis and experimentation to investigate the effect of particles on cavitation. The simulated results regarding velocity and pressure gradients, turbulence quantities, and vapor volume fractions are critically analyzed and discussed. The cavitation number changes to 0.22 in the experiment and 0.16 in the simulation due to the presence of biomass particles. Orifice plate thickness was found to significantly influence cavitation inception and evolution. 4 mm thickness and nine holes with specific orientations were found to be an optimized geometry with the lowest cavitation number, maximum pressure drop, and highest throat velocity. In the experimentation, 0.8 mm biomass particles were used in the mixture (2 % w/w) to determine the effect of biomass particles on the flow. This result helps identify the critical operating and design parameters and the impact on the cavitation of particles to achieve the desired cavitation phenomena.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00925"},"PeriodicalIF":0.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodecolorization and Biodegradation of Methyl Orange by Immobilized Pseudomonas aeruginosa Bacterium into SA/PVA Matrix Integrated with MOF UiO-66 Adsorbent","authors":"Sarazen Shalahuddin Akbar,&nbsp;Adi Setyo Purnomo ,&nbsp;Silvia Abdi Pratama,&nbsp;Berlian Imazdalifa,&nbsp;Sifra Thrivelia Krisnia Ayu,&nbsp;Reyza Fadly Maghfiroh,&nbsp;Nur Annisa Kusumawardhani,&nbsp;Ardi Lukman Hakim","doi":"10.1016/j.btre.2025.e00924","DOIUrl":"10.1016/j.btre.2025.e00924","url":null,"abstract":"<div><div>Azo dye, such as methyl orange (MO), is characterized by environmental concerns due to persistence, toxicity, and potential carcinogenic effects. Therefore, this study aimed to investigate the integration of immobilized <em>Pseudomonas aeruginosa</em> (PA) and UiO-66, a zirconium-based metal-organic framework (MOF), into sodium alginate/polyvinyl alcohol (SA/PVA) matrix for biodecolorization and biodegradation of MO dye. Material characterization was carried out by using X-ray diffraction (XRD), Fourier-transform infrared (FTIR), and scanning electron microscopy-energy dispersive X-ray (SEM-EDX) which showed the successful formation of UiO-66 and stable incorporation in SA/PVA/PA beads. The results of adsorption experiments showed that the presence of UiO-66 enhanced MO adsorption capacity by 20.47 % compared to SA/PVA/PA matrix without UiO-66. Kinetic analysis followed the pseudo-second-order model, and adsorption isotherm was best described by the Langmuir model, showing monolayer adsorption behavior. Furthermore, biodecolorization results showed that SA/PVA/UiO-66/PA beads achieved a 92.03 % MO removal rate, indicating a 33.99 % higher than free PA cells. Liquid chromatography–mass spectrometry (LC-MS) analysis confirmed the formation of nine biodegradation products, suggesting successful structural breakdown of MO. These results showed that combining UiO-66 with immobilized PA significantly enhanced both adsorption and biodegradation performance toward MO treatment.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00924"},"PeriodicalIF":0.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solving the light chain mismatch of IgG-like bispecific antibody by utilizing 2A peptide based FaBody platform","authors":"Dong Li ,&nbsp;Pengfan Zheng ,&nbsp;Xuejing Yao ,&nbsp;Baopeng Zhang ,&nbsp;Jiekun Zhang ,&nbsp;Yaocheng Qu ,&nbsp;Shasha Yun ,&nbsp;Yanzhen Li ,&nbsp;Shanshan Chen ,&nbsp;Jianmin Fang","doi":"10.1016/j.btre.2025.e00917","DOIUrl":"10.1016/j.btre.2025.e00917","url":null,"abstract":"<div><div>Bispecific antibodies (BsAbs), engineered to target multiple antigens or epitopes simultaneously, promise enhanced therapeutic efficacy over traditional monoclonal antibodies (mAbs). However, the complex BsAbs structure presents significant production challenges, particularly chain mismatch issues. This study presents a novel approach utilizing 2A peptides within a FaBody platform to address light chain mismatches in IgG-like BsAbs. By leveraging self-cleaving 2A peptides, stable expression in mammalian cells significantly improves the accuracy of antibody chain assembly. This strategy markedly enhances the production of correctly assembled IgG-like BsAbs, providing a promising solution to critical challenges in BsAbs drug development.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00917"},"PeriodicalIF":0.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic analysis of underutilized legumes: insights into evolutionary relationships, genome evolution and stress tolerance","authors":"Omena Bernard Ojuederie ,&nbsp;Ufuoma Lydia Akpojotor ,&nbsp;Adetomiwa Ayodele Adeniji ,&nbsp;Tina Chukwuyem Ojuederie ,&nbsp;Jacob Olagbenro Popoola ,&nbsp;Olubukola Oluranti Babalola","doi":"10.1016/j.btre.2025.e00918","DOIUrl":"10.1016/j.btre.2025.e00918","url":null,"abstract":"<div><div>African yam bean, Mung bean, and Winged bean, which are rich sources of nutrients and bioactive compounds, offer significant potential for food and nutrition security, yet they are underutilized. A comparative genomic analysis of these legumes with cowpea was conducted to unearth their molecular architecture and uncover their rich genomic profile. Protein and genomic fasta sequences were retrieved from the GenBank of the NCBI, and orthologous genes investigated, and secondary metabolites determined using OrthoVenn3 and PlantiSMASH programs. A total of 7761 single-copy and 20,250 unique genes were identified, which revealed their genetic diversity and conservation. Phylogenetic analysis showed the closest relationship between Cowpea and Mung bean, with Winged bean diverging significantly. Cowpea and Mung bean had significant gene expansions (+1051), while African yam bean (-864) and Winged bean (-643) had substantial gene losses. GO enrichment revealed the contributions to adaptations in the different legume species to biotic and abiotic stresses, highlighting their potential as climate-resilient crops. The highest protein gene (enzyme) count for saccharide (68) and terpene (18) biosynthesis was obtained in AYB. At the same time, mung bean had the highest gene clusters for alkaloids (10) and polyketides (5), and the highest enzyme count for the biosynthesis of alkaloids (32) and polyketides (17). Underutilized legumes exhibited higher essential amino acid levels compared to cowpea. These findings provide valuable insights for breeding programs and biotechnological interventions to improve the nutritional value and acceptance of these underutilized legumes, ultimately contributing to food and nutrition security.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00918"},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating a new Dillenia ovata endophytic bacteria to produce antioxidants and anti-diabetes activity in vitro and in vivo","authors":"Linh Chi Tran ,&nbsp;Chong Kim Thien Duc ,&nbsp;Tuan Trong Nguyen ,&nbsp;Duy Toan Pham ,&nbsp;Danh Thai Luu ,&nbsp;Trang Thi Xuan Dai","doi":"10.1016/j.btre.2025.e00921","DOIUrl":"10.1016/j.btre.2025.e00921","url":null,"abstract":"<div><div>A novel endophytic bacterium, <em>Bacillus</em> sp. DO-R5, with strong antioxidant and anti-diabetic properties, was isolated from <em>Dillenia ovata</em>, a traditional Vietnamese medicinal plant. <em>Bacillus</em> sp. DO-R5 was identified using morphological characteristics, SEM, and <em>16S rRNA</em> sequencing, demonstrated the highest bioactivity among the 19 isolates. Optimal fermentation conditions (8.9 g/L D-glucose, pH 7.5, 38 °C, and 70.5 h) were determined using the Box-Behnken design. Under these conditions, <em>Bacillus</em> sp. DO-R5 significantly enhanced the production of bioactive compounds including diosmin, rutin, and chlorogenic acid, and exhibited improved <em>in vitro</em> antioxidant, anti-inflammatory, and anti-diabetic activities. <em>In vivo</em>, the extract effectively reduced blood glucose, regulated lipid profiles, supported weight control, and protected organs. These findings emphasize the potential therapeutic use of <em>D. ovata</em> endophytic bacteria as a natural source of anti-diabetic drugs.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00921"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sargassum incisifolium and Ulva spp metabolites activity and their molecular dynamics simulation against Fusarium oxysporum 14-alpha-demethylase","authors":"Omolola Aina ,&nbsp;Adewale O. Fadaka ,&nbsp;Daniel Watson ,&nbsp;Cecilia Y. Ojemaye ,&nbsp;Denzil R. Beukes ,&nbsp;Kudakwashe Nyambo ,&nbsp;Kudzanai Tapfuma ,&nbsp;Vuyo Mavumengwana ,&nbsp;Nicole R. S Sibuyi ,&nbsp;Marshall Keyster ,&nbsp;Ashwil Klein","doi":"10.1016/j.btre.2025.e00919","DOIUrl":"10.1016/j.btre.2025.e00919","url":null,"abstract":"<div><div>Fusarium oxysporum, a major agricultural pathogen, poses severe risks to crops worldwide. With increasing resistance to conventional antifungal agents, there is an urgent need for alternative treatments. Seaweeds such as <em>Ulva spp.</em> and Sargassum incisifolium are promising sources of bioactive compounds that may offer novel antifungal properties. This study investigates the antifungal activity of acetone extracts from <em>Ulva spp.</em> and Sargassum incisifolium against <em>F. oxysporum</em>, with the goal of identifying specific bioactive compounds responsible for this activity and evaluating their effectiveness quantitatively. We prepared acetone extracts from both seaweed species and assessed their antifungal activity using a series of in vitro assays. The total phenolic content (TPC) and antioxidant capacity were determined for each extract. LC-qTOF-MS/MS was employed for phytochemical profiling, while molecular docking and molecular dynamics simulations were used to predict interactions between identified compounds and the 14-alpha-demethylase enzyme of <em>F. oxysporum</em>. The TPC was 2.72±0.009 and 2.23±0.009 GAE/mg dry weight for <em>Ulva spp</em> and <em>S. incisifolium</em>. Additionally, significant antioxidant activity was observed, with IC50 values of 8.38±0.06 µg/mL for <em>Ulva spp</em> and 8.01±0.07 µg/mL for <em>S. incisifolium</em>, which are comparable to ascorbic acid (5.23±0.04 µg/mL). Phytochemical analysis revealed high levels of terpenoids, phenolics, and fatty acids. In molecular docking, compounds such as medicocarpin, corynanthine, and merulinic acid demonstrated strong binding affinities (binding energies ≤ -7.5 kcal/mol). Molecular dynamics simulations confirmed stable interactions over 100 ns, with medicocarpin exhibiting the most stable binding profile. The study demonstrates that acetone extracts of <em>Ulva spp.</em> and <em>S. incisifolium</em> possess significant antifungal activity against <em>F. oxysporum</em>. Medicocarpin, in particular, emerged as a promising candidate for further development as an antifungal agent. These findings underscore the potential of seaweed-derived compounds as antifungal agents against fungal pathogens and highlight the need for further investigation into their practical applications in plant disease management. Specifically, Medicocarpin emerged as a promising <em>in silico</em> candidate, warranting further experimental validation.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00919"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospecting technology and economic potential to produce bio-succinic acid: a review","authors":"Diniz A.S. Silva ,&nbsp;Rafael Luque ,&nbsp;Yaimé Delgado-Arcaño ,&nbsp;Elen A. Perpetuo ,&nbsp;Alisson D. da S. Ruy ,&nbsp;Ana Lucia B. de Souza ,&nbsp;Luiz A.M. Pontes","doi":"10.1016/j.btre.2025.e00920","DOIUrl":"10.1016/j.btre.2025.e00920","url":null,"abstract":"<div><div>Succinic acid (SA) is an organic acid and a key building block with various synthetic applications in the food, pharmaceutical, and chemical industries. Currently produced through petrochemical routes, the use of microorganisms to produce it from biomass waste offers a renewable and sustainable alternative. This study evaluates the main technologies for producing succinic acid via biotechnological routes (BioSA) and its market potential, aiming for commercial production. Research highlights BioSA’s competitive edge and significant capacity to synthesize high-value products, such as 1,4-butanediol and polybutylene succinate (PBS), offering a viable alternative to fossil-derived maleic anhydride. Among BioSA-producing microorganisms, <em>Escherichia coli</em> and <em>Actinobacillus succinogenes</em> are the most promising, achieving titers of 1.10 and 1.23 gg^-1, respectively, and yields of 127.1 and 83.7 gL^-1 under anaerobic conditions at low pH, with genetic modifications to maximize productivity. The market is expected to grow at an annual rate of up to 6.7 % through 2032.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00920"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145158222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}