Biotechnology Reports最新文献

{"title":"Investigation and optimization of DNA isolation efficiency using ferrite-based magnetic nanoparticles","authors":"Tímea B. Gerzsenyi ,&nbsp;Ágnes M. Ilosvai ,&nbsp;Ferenc Kristály ,&nbsp;Lajos Daróczi ,&nbsp;Michael C. Owen ,&nbsp;Béla Viskolcz ,&nbsp;László Vanyorek ,&nbsp;Emma Szőri-Dorogházi","doi":"10.1016/j.btre.2025.e00904","DOIUrl":"10.1016/j.btre.2025.e00904","url":null,"abstract":"<div><div>DNA isolation is a crucial step in many molecular biological applications for diagnostic and research purposes, like detection of infectious diseases or gene expression studies. However, due to the requirement of toxic reagents in traditional procedures and the high expenses of commercial kits, the use of magnetic MNP-based DNA isolation is becoming more widespread. In this study, different ferrite containing MNPs (MnFe₂O₄, MnFe₂O₄-NH₂, MgFe₂O₄, MgFe₂O₄-NH₂ NiFe₂O₄, NiFe₂O₄-NH₂) are examined and compared in their pDNA isolation efficiency. Among the tested nanoparticles, we document the use of NiFe₂O₄ and its amine-functionalized form for the first time. Three protocols for the isolation of pDNA are optimized for each type of nanoparticle and the best protocol is selected based on the quantity, quality and integrity of the extracted DNA. Plasmid samples extracted with the MNPs are transformed into competent bacterial cells and further tests are performed to recover genomic DNA from bacterial cells, leading to the development of another protocol. Bacteria-spiked blood serum samples are produced to extract DNA from a more complex biological matrix.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00904"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis of RAS signaling effectors reveals stage-dependent oncogenic patterns in colon adenocarcinoma","authors":"Loretta László ,&nbsp;Anna Lovrics ,&nbsp;Álmos Tilajka ,&nbsp;Tamás Takács ,&nbsp;László Buday ,&nbsp;Virag Vas","doi":"10.1016/j.btre.2025.e00902","DOIUrl":"10.1016/j.btre.2025.e00902","url":null,"abstract":"<div><div>Cancer rarely results from a single gene defect but emerges from disruptions in complex cellular networks. The Network Medicine perspective guides our investigation of cancer-driving interactions, particularly focusing on RAS signaling pathways that are key mediator for cancer development.</div><div>We analyzed gene expression patterns in colon and lung cancers to identify stage-specific molecular drivers. Using computational modelling combined with patient tissue analysis, we discovered five key genes that are specifically altered in early-stage colon cancer: RAF1, PLCE1, RGL1, RIN1, and GRB7. These genes work as RAS effectors in signaling and can effectively distinguish between normal and cancerous colon tissue.</div><div>Our approach combines network analysis with gene expression studies to understand how RAS signaling disruption contributes to colon cancer development. These findings suggest that targeting early-stage RAS-related changes could offer therapeutic opportunities before cancer becomes more complex and harder to treat.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00902"},"PeriodicalIF":0.0,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scale-down optimization of a robust, parallelizable human induced pluripotent stem cell bioprocess for high-throughput research","authors":"James Colter ,&nbsp;Tiffany Dang ,&nbsp;Julia Malinovska ,&nbsp;Jessica May Corpuz ,&nbsp;Dora Modrcin ,&nbsp;Roman Krawetz ,&nbsp;Kartikeya Murari ,&nbsp;Michael Scott Kallos","doi":"10.1016/j.btre.2025.e00900","DOIUrl":"10.1016/j.btre.2025.e00900","url":null,"abstract":"<div><div>Human induced pluripotent stem cell (hiPSC) derived therapeutics require clinically relevant quantities of high-quality cell populations for applications in regenerative medicine. The lack of efficacy exhibited across clinical trials suggests deeper understanding of the networks governing phenotype is needed. Further, costs limit study throughput in characterizing the artificial niche relative to outcomes. We present herein an optimized strategy to enable high-throughput hiPSC expansion at &lt;20 mL research scale. We assessed viability of single cell inoculation and aggregate preformation to facilitate proliferation. We modeled aggregate characteristics against agitation rate. Our results demonstrate tunable control with fold expansion comparable to commercial systems. Marker quantification and teratoma assay confirm functional pluripotency. This approach constitutes a scalable protocol to accelerate hiPSC research, and a significant step in advancing the rate of progress in elucidating links to derivative functionality. This work will enable statistically rigorous studies targeting hiPSC and downstream phenotype for clinical manufacturing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00900"},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of Cupriavidus necatorstrains on hydrolyzed lignocellulosic feedstocks widely available in Europe","authors":"Halima Aliyu Alhafiz ,&nbsp;Karin Longus ,&nbsp;Rob A.J. Verlinden ,&nbsp;Vera Lambauer ,&nbsp;Andreas Kruschitz ,&nbsp;Regina Kratzer","doi":"10.1016/j.btre.2025.e00899","DOIUrl":"10.1016/j.btre.2025.e00899","url":null,"abstract":"<div><div>Today, 85 % of the carbon in organic chemicals and their derivatives comes from fossil sources. Replacing fossil-based materials with sustainable sources requires large quantities of feedstocks and mature technologies. Biorefineries based on lignocellulose have great potential to replace fossil raw materials in the short and medium term. Here we want to pave the way for the bacterium <em>Cupriavidus necator</em> as a versatile biotechnological workhorse in future biorefineries. Wheat straw, beech, pine and spruce reflect lignocellulosic biomass from the agricultural waste and wood sectors that is widespread in Europe. Miscanthus was chosen as an emerging energy crop. Lignocellulose feedstocks were pretreated by steam explosion under variable conditions prior to enzymatic hydrolysis. Native <em>Cupriavidus necator</em> and a strain adapted by laboratory evolution were shown to grow on 16 filtered lignocellulosic hydrolysates as the sole carbon source and without prior detoxification.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00899"},"PeriodicalIF":0.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction conditions that promote the effect of glycerol on recombinant protein production in Escherichia coli","authors":"Yoshihiro Ojima ,&nbsp;Hajime Saito ,&nbsp;Shintaro Miyuki ,&nbsp;Koichi Fukunaga ,&nbsp;Terumichi Tsuboi ,&nbsp;Masayuki Azuma","doi":"10.1016/j.btre.2025.e00898","DOIUrl":"10.1016/j.btre.2025.e00898","url":null,"abstract":"<div><div>Proinsulin was expressed by <em>Escherichia coli</em> SHuffle T7 with pET system in minimal medium containing glucose (Glu medium), glucose and glycerol (GluGly medium) or glycerol (Gly medium). With 100 μM IPTG, proinsulin production did not increase with glycerol. In contrast, proinsulin production per medium volume in GluGly and Gly media was approximately 3∼4-fold higher than in Glu medium with 10 μM IPTG. mRNA expression of target protein was higher in GluGly versus Glu medium, indicating that proinsulin production was enhanced by the release of glucose-induced catabolite inhibition. Although proinsulin production did not differ between GluGly and Gly media at 25 h, substrate was consumed quickly in GluGly medium with 1.55±0.12 times higher proinsulin production at 15 h. Productivity, considering the production period, was highest in the GluGly medium. This study shows a mixture of glucose and glycerol is valuable for protein production in <em>E. coli</em> with low IPTG concentrations.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00898"},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and evaluation of rabies immunoglobulin extracted from chicken egg yolk","authors":"Vichununt Kerdput ,&nbsp;Vararut Yodkamol ,&nbsp;Mattika Sookprasong ,&nbsp;Wongsakorn Wongwadhunyoo ,&nbsp;Iyacoob Khunsri ,&nbsp;Promsin Masrinoul ,&nbsp;Trong Wisedchanwet ,&nbsp;Naphatsamon Uthailak ,&nbsp;Yanin Limpanont ,&nbsp;Onrapak Reamtong ,&nbsp;Poom Adisakwattana ,&nbsp;Charin Thawornkuno","doi":"10.1016/j.btre.2025.e00897","DOIUrl":"10.1016/j.btre.2025.e00897","url":null,"abstract":"<div><div>This study investigated the potential of using laying hens to produce rabies immunoglobulin (RIG) due to concerns about an African Horse Sickness outbreak in 2020. The hens were immunized with rabies vaccine, resulting in the production of Immunoglobulin Y (IgY) antibodies in their egg yolks. The study found that using polyethylene glycol 6000 (PEG) was the most effective method for extracting anti-rabies IgY from egg yolks in terms of amount (1.87 mg of IgY from one mL of egg yolk), purity (83 %), and cost-effectiveness. Further purification through affinity chromatography increased the purity to 95 %, as confirmed by 1D GeLC-MS/MS, showing a purity of up to 98 %. In addition, IgY extracted using PEG demonstrated the highest neutralizing activity compared to the caprylic acid and ammonium sulfate methods. It was estimated that only 8 eggs are needed to produce a vial of RIG with the same potency as equine RIG (1000 IU/vial).</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00897"},"PeriodicalIF":0.0,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic copper and copper oxide nanoparticles to combat multidrug-resistant Staphylococcus aureus: Green synthesis, mechanisms, resistance, and future perspectives","authors":"Gamal M. El-Sherbiny,&nbsp;M.E. Shehata,&nbsp;Mohamed H. Kalaba","doi":"10.1016/j.btre.2025.e00896","DOIUrl":"10.1016/j.btre.2025.e00896","url":null,"abstract":"<div><div>Antimicrobial resistance has increased alarmingly in recent years, with the World Health Organization identifying multidrug-resistant <em>Staphylococcus aureus</em> as a particular threat to global public health due to its extensive resistance profile and associated high mortality rates. While various metal nanoparticles have been explored as antimicrobial agents, the specific advantages of biosynthesized copper nanoparticles against MDR <em>S. aureus</em> remain inadequately consolidated in the literature.</div></div><div><h3>Objective</h3><div>This review uniquely evaluates the emerging evidence for biosynthesized copper nanoparticles as a sustainable, cost-effective, and potentially alternative to conventional antibiotics against multidrug-resistant <em>S. aureus</em> strains.</div></div><div><h3>Methods</h3><div>We systematically analyzed current literature on green synthesis methods for copper and copper oxide nanoparticles, their characterization techniques, antimicrobial mechanisms, and efficacy against multidrug-resistant <em>S. aureus</em>, focusing on identifying knowledge gaps and future research directions.</div></div><div><h3>Results</h3><div>Unlike other metal nanoparticles, biosynthesized copper nanoparticles demonstrate significant antibacterial activity against multidrug-resistant <em>S. aureus</em> through multiple simultaneous mechanisms that bacteria try to develop resistance against. Their unique physicochemical properties enable enhanced bacterial elimination compared to conventional antibiotics and other metal nanoparticles, with minimal toxicity to mammalian cells at therapeutic concentrations. Our analysis further reveals the considerable potential of these nanoparticles to overcome existing biological barriers in infection sites that limit conventional therapies.</div></div><div><h3>Conclusion</h3><div>This broad assessment of biosynthesized copper nanoparticles shows strong potential as a therapy against MDR <em>S. aureus</em> and provides a foundation for future research to address antimicrobial resistance where current treatments fail.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00896"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of microbial contamination and biogenic amines formation in Ras cheese using Chlorella vulgaris extracts","authors":"Diaa A. Marrez","doi":"10.1016/j.btre.2025.e00895","DOIUrl":"10.1016/j.btre.2025.e00895","url":null,"abstract":"<div><div>The present study aimed to explore the impact of <em>C. vulgaris</em> aqueous and diethyl ether extracts on reducing microbial contamination and biogenic amines (BAs) formation in Ras cheese during ripening process. Phenolic profile of aqueous extract was quantified by high performance liquid chromatography (HPLC) and compounds in ether extract were determined using gas chromatography-mass spectrometry (GC–MS). Eleven compounds were detected in aqueous extract and catechin was the major (54.82 µg/g), while 14 compounds were detected in ether extract of which the main compounds hexadecane (14.26 %) and 9, 12-Octadecadienoic acid, methyl ester (9.61 %). <em>C. vulgaris</em> aqueous and ether extracts observed antibacterial activity against seven strains of foodborne bacteria with minimum inhibitory concentration (MIC) ranged from 0.33 to 1.43 mg/mL and antifungal activity against 9 strains of toxigenic fungi with MIC values from 0.42 to 1.74 mg/mL. Ras cheese treated with ether extract had the highest reduction in formation of tryptamine (93.4 %), β-phenylethylamine (86.4 %), Putrescine (88.3 %), Cadaverine (81.8 %), Histamine (62.9 %), Serotonin (92.1 %), Tyramine (87.2 %), Spermidine (80.8) and Spermine (86.8 %) compared to cheese treated with aqueous extract and control samples. Also, the microbial load (total bacterial count, yeasts and molds count, proteolytic bacterial count) in Ras cheese samples treated with <em>C. vulgaris</em> aqueous and diethyl ether extracts were lower than the control sample. Whereas, no growth of coliform group, Staphylococci and Salmonella were detected in treated samples and control. The microalga <em>C. vulgaris</em> extracts considered promising source from natural ingredients to reduce biogenic amines content and microbial load in cheese manufacturing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00895"},"PeriodicalIF":0.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the potential biochemical effects of selected heterocyclic compounds as human Type-A γ-aminobutyric acid (GABA A) Modulator: An Insilico Approach","authors":"Abel Kolawole Oyebamiji ,&nbsp;Sunday Adewale Akintelu ,&nbsp;Oluwakemi Ebenezer ,&nbsp;Faith Eniola Olujinmi ,&nbsp;David O. Adekunle ,&nbsp;Adesoji Alani Olanrewaju ,&nbsp;Omowumi Temitayo Akinola ,&nbsp;Samson Olusegun Afolabi ,&nbsp;Ehimen Anastasia Erazua ,&nbsp;Ayodeji Arnold Olaseinde","doi":"10.1016/j.btre.2025.e00894","DOIUrl":"10.1016/j.btre.2025.e00894","url":null,"abstract":"<div><h3>Background</h3><div>Investigating the bioactivities of zuranolone derivatives as Type-A γ-aminobutyric acid inhibitors which will thereby down-regulate postpartum depression is considered a crucial study.</div></div><div><h3>Method</h3><div>This study is aimed at investigating the biochemical activities of 1-(2-((3R,5R,8R,9R,10S,13S,14S,17S)-3‑hydroxy-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-oxoethyl)-1H-pyrazole-4-carbonitrile derivatives against type-A γ-aminobutyric acid (GABA A) which will thereby enhance the activity of γ-aminobutyric acid (GABA) in human central nervous system.</div></div><div><h3>Results</h3><div>In this work, series of computational tools such as Spartan 14, molecular operating environment software, Gromacs and Admetsar1 were explored and the studied compounds were subjected to this software which resulted to series of results. Vacuum was observed to have highest influence on highest occupied molecular orbital (E_H) of compound 1 and water as well as ethanol reduces its ability to donate electron to the nearby molecules. Also, the effect of water and ethanol were investigated on the studied compound via lowest unoccupied molecular orbital (E_L) and energy gap and the results were reported appropriately. The molecular docking investigation was carried out on the studied compounds and Type-A γ-aminobutyric acid (pdb id: 4cof) and the compounds 3 with calculated binding affinity value of -7.32433319kcal/mol as well as pi-H as the non-bonding interaction were observed which therefore confirm the potential ability of compound to inhibit the target than other studied compound. Also, compound 1 and reference compound were subjected to molecular dynamic simulation study and the actual binding energy for the selected compounds were obtained and reported.</div></div><div><h3>Conclusion</h3><div>Our findings from this work may open door for the design of several 1-(2-((3R,5R,8R,9R,10S,13S,14S,17S)-3-(benzyloxy)-3,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-oxoethyl)-1H-pyrazole-4-carbonitrile derivatives as potential Type-A γ-aminobutyric acid inhibitors.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00894"},"PeriodicalIF":0.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut","authors":"Sivakumar Madhan ,&nbsp;Yuvasri Errakutty Arunan ,&nbsp;Anandham Rangasamy ,&nbsp;Balachandar Dananjeyan ,&nbsp;Johnson Iruthayasamy ,&nbsp;Manimaran Gajendiran ,&nbsp;Krishnamoorthy Ramasamy ,&nbsp;Raghu Rajasekaran ,&nbsp;Vincent Saminathan","doi":"10.1016/j.btre.2025.e00893","DOIUrl":"10.1016/j.btre.2025.e00893","url":null,"abstract":"<div><div>Quorum sensing (QS) mechanisms play an essential role in mediating several signals and plant-bacteria interactions, promoting plant growth. This study demonstrated production of multiple Homoserine lactone (HSL) molecules like C6 HSL, C7 HSL, C8 HSL, 3-Hydroxy-C8-HSL and 3-oxo-C14 HSL in rhizobial and passenger endophytes and phyllospheric bacteria which regulated production of plant growth promoting traits <em>viz.,</em> indole acetic acid and exo-polysaccharide production, biofilm formation, and motility. Quorum quenching (QQ) molecules like salicylic acid, gallic acid, and disalicylic acid impaired these traits, but exogenous addition of QS molecules (C7HSL and 3-oxo-C14 HSL) restored these inhibitory effects of QQ compounds. The pot culture experiment revealed that the treatment involving <em>Methylobacterium populi</em> TMV7–4 or <em>Enterobacter cloacae</em> S23 with salicylic acid, C7HSL and 3-oxo-C14 HSL significantly enhanced plant growth including root length, nodulation, pod formation, soil available nutrients and plant nutrients uptake. In future field validation is required for the use of QS molecules in improving groundnut production.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00893"},"PeriodicalIF":0.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}