利用基于2A肽的FaBody平台解决igg样双特异性抗体轻链错配问题

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2025-08-30 DOI:10.1016/j.btre.2025.e00917

Dong Li , Pengfan Zheng , Xuejing Yao , Baopeng Zhang , Jiekun Zhang , Yaocheng Qu , Shasha Yun , Yanzhen Li , Shanshan Chen , Jianmin Fang

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引用次数: 0

摘要

双特异性抗体（BsAbs）可以同时靶向多种抗原或表位，有望比传统的单克隆抗体（mab）具有更好的治疗效果。然而，复杂的bsab结构带来了重大的生产挑战，特别是链不匹配问题。本研究提出了一种利用FaBody平台中的2A肽来解决igg样bsab轻链错配的新方法。通过利用自切割2A肽，在哺乳动物细胞中的稳定表达显著提高了抗体链组装的准确性。这一策略显著提高了正确组装igg样bsab的生产，为bsab药物开发中的关键挑战提供了一个有希望的解决方案。

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Solving the light chain mismatch of IgG-like bispecific antibody by utilizing 2A peptide based FaBody platform

Bispecific antibodies (BsAbs), engineered to target multiple antigens or epitopes simultaneously, promise enhanced therapeutic efficacy over traditional monoclonal antibodies (mAbs). However, the complex BsAbs structure presents significant production challenges, particularly chain mismatch issues. This study presents a novel approach utilizing 2A peptides within a FaBody platform to address light chain mismatches in IgG-like BsAbs. By leveraging self-cleaving 2A peptides, stable expression in mammalian cells significantly improves the accuracy of antibody chain assembly. This strategy markedly enhances the production of correctly assembled IgG-like BsAbs, providing a promising solution to critical challenges in BsAbs drug development.

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.