Biotechnology Reports最新文献_第2页

Design of a GFP reporter for splicing analysis in mammalian cells 用于哺乳动物细胞剪接分析的GFP报告基因的设计

Biotechnology Reports Pub Date : 2025-03-13 DOI: 10.1016/j.btre.2025.e00887

Arthur T. Menezes, Helder Y. Nagasse, Hilan R. M. Lopes, Patricia P. Coltri

引用次数: 0

Exploring the multifaceted roles of resuscitation-promoting factors in tuberculosis: Implications for diagnosis, vaccine development, and drug targeting 探讨复苏促进因素在结核病中的多方面作用：对诊断、疫苗开发和药物靶向的影响

Biotechnology Reports Pub Date : 2025-03-13 DOI: 10.1016/j.btre.2025.e00886

Gamze Tanriver , Salman Ali Khan , Artur Góra , Novel N Chegou , Shima Mahmoudi

{"title":"Exploring the multifaceted roles of resuscitation-promoting factors in tuberculosis: Implications for diagnosis, vaccine development, and drug targeting","authors":"Gamze Tanriver , Salman Ali Khan , Artur Góra , Novel N Chegou , Shima Mahmoudi","doi":"10.1016/j.btre.2025.e00886","DOIUrl":"10.1016/j.btre.2025.e00886","url":null,"abstract":"<div><div>Tuberculosis (TB) remains a significant global health challenge, necessitating continued research efforts to unravel its complex pathogenesis and advance diagnostic, therapeutic, and preventive strategies. Resuscitation-promoting factors (Rpfs) are peptidoglycan-hydrolyzing enzymes that have gained attention due to their key roles in TB infection dynamics. This review aims to provide a comprehensive overview of Rpfs in TB, highlighting their immunological roles, diagnostic potential, and implications for vaccine development and drug targeting through both in silico and experimental approaches. Rpfs exhibit diverse roles in TB, influencing bacterial resuscitation from dormancy, and immune modulation through interactions with host immune cells, such as dendritic cells, and they are potential targets for novel diagnostic and therapeutic interventions. Their ability to stimulate immune responses, particularly interferon-γ production by T cells, underscores their potential as vaccine candidates against TB. Moreover, Rpfs represent promising targets for drug discovery, with inhibitors potentially disrupting bacterial resuscitation and growth.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00886"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells 用于分离和扩增人膀胱平滑肌细胞的无异种平台

Biotechnology Reports Pub Date : 2025-02-11 DOI: 10.1016/j.btre.2025.e00878

Teresa Franchi-Mendes , Marília Silva , Maria Catarina Carreira , Ana Luísa Cartaxo , Paulo Vale , Panagiotis Karakaidos , Apostolos Klinakis , Ana Fernandes-Platzgummer , Cláudia L. da Silva

{"title":"Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells","authors":"Teresa Franchi-Mendes , Marília Silva , Maria Catarina Carreira , Ana Luísa Cartaxo , Paulo Vale , Panagiotis Karakaidos , Apostolos Klinakis , Ana Fernandes-Platzgummer , Cláudia L. da Silva","doi":"10.1016/j.btre.2025.e00878","DOIUrl":"10.1016/j.btre.2025.e00878","url":null,"abstract":"<div><h3>Introduction</h3><div>Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers.</div></div><div><h3>Materials & Methods</h3><div>A gamma-irradiated human platelet lysate (HPL) supplement was used to develop a xeno(geneic)-free process for the isolation and scalable expansion of human bladder-derived SMC.</div></div><div><h3>Results</h3><div>Cells were isolated using an explant-based technique and expanded ex vivo, expressing typical SMC markers (α-SMA, desmin, caldesmon and SM22-α). Cell culture was successfully scaled-up using spinner flasks combined with plastic microcarriers, starting with a 2.8 × 10<sup>3</sup> cells/cm<sup>2</sup> inoculum (i.e. 1 × 10<sup>6</sup> cells). Cell-microcarrier adhesion rates exceeded 80% within 24 hours with fold expansion ranging from 3.5 to 16.8 after 7 days, dependent on donor variability. Harvested cells retained their SMC phenotype.</div></div><div><h3>Conclusions</h3><div>This xeno-free, GMP compliant process enables scalable manufacturing of human bladder-derived SMC while preserving cell identity, potentially advancing clinical applications in bladder engineering.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00878"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding black soldier fly (BSF; Hermetia illucens; Diptera: Stratiomyidae) in the developing world: Use of BSF larvae as a biological tool to recycle various organic biowastes for alternative protein production in Nepal 扩大黑兵蝇(BSF；Hermetia illucens;双翅目：层蛾科)在发展中国家：在尼泊尔，利用BSF幼虫作为一种生物工具，回收各种有机生物废物，用于替代蛋白质生产

Biotechnology Reports Pub Date : 2025-02-10 DOI: 10.1016/j.btre.2025.e00879

Bhola Gautam , Sundar Tiwari , Min Raj Pokhrel , Jeffery K. Tomberlin , Prabhat Khanal

{"title":"Expanding black soldier fly (BSF; Hermetia illucens; Diptera: Stratiomyidae) in the developing world: Use of BSF larvae as a biological tool to recycle various organic biowastes for alternative protein production in Nepal","authors":"Bhola Gautam , Sundar Tiwari , Min Raj Pokhrel , Jeffery K. Tomberlin , Prabhat Khanal","doi":"10.1016/j.btre.2025.e00879","DOIUrl":"10.1016/j.btre.2025.e00879","url":null,"abstract":"<div><div>The growing global demand for food, particularly animal protein, is intensifying challenges related to food security and environmental sustainability. The increase in organic waste generation, coupled with inefficient waste management, is further deteriorating living conditions by negatively impacting the environment and public health, especially in developing nations. This study investigated the potential of black soldier fly larvae (BSFL) to recycle major daily organic waste fractions in Nepal. BSFL were exposed to seven different biowaste-based substrates locally sourced from fruit and vegetable markets, farms, and food industries. Additionally, the study evaluated rapeseed cake as a supplement to enhance BSFL growth and nutritional content. BSFL survival rates exceeded 80 % on food industry waste but dropped to 63 % on high-moisture substrates like vegetable waste. Mixed vegetable waste (14.7 mg/day) and bakery waste (11.5 mg/day) supported higher average daily weight gain, likely due to their better nutrient values (soluble carbohydrates and proteins). Although rapeseed cake alone hindered larval growth, its supplementation to biowastes improved growth, survival, and bioconversion rates, increased larval protein content up to 32 %, and reduced fat by 36 %. These findings indicate BSFL can effectively recycle diverse, locally available organic wastes in developing countries like Nepal, providing a sustainable source of domestic protein and contributing to feed security. As this is the first BSFL study in Nepal, further research is needed to elucidate the chemical and microbial safety of BSFL reared on biowastes and to develop technical solutions for commercial BSFL production in countries with a low-income economy.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00879"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae strains isolated from piggery wastewater in Ecuador: Effective nitrogen compound removal and growth potential in extremophile conditions 从厄瓜多尔养猪场废水中分离的微藻菌株：在极端微生物条件下有效去除氮化合物和生长潜力

Biotechnology Reports Pub Date : 2025-02-10 DOI: 10.1016/j.btre.2025.e00883

Karla Flores-Zambrano , Wilson Tapia , Pablo Castillejo

{"title":"Microalgae strains isolated from piggery wastewater in Ecuador: Effective nitrogen compound removal and growth potential in extremophile conditions","authors":"Karla Flores-Zambrano , Wilson Tapia , Pablo Castillejo","doi":"10.1016/j.btre.2025.e00883","DOIUrl":"10.1016/j.btre.2025.e00883","url":null,"abstract":"<div><div>Effluents generated by anthropogenic activities are a significant source of pollution and eutrophication in natural water bodies. In Ecuador, the increase in pig production has exacerbated this issue due to the untreated discharge of pig effluents. This study focused on the characterization of native microalgae present in pig effluents and the evaluation of their capacity to remove nitrogenous compounds under various conditions, with the aim of identifying efficient strains for phycoremediation. Four microalgal strains were isolated and molecularly identified as <em>Radiococcus polycoccus, Chlorolobion braunii, Micractinium</em> sp., and <em>Desmodesmus multivariabilis</em>. The cultures were exposed to initial concentrations of 100 mg L<sup>-1</sup> N-NH₄ and 49.97 mg L<sup>-1</sup> N-NO₃ for 12 days to assess their cellular growth and nutrient removal rates. Growth kinetics were analyzed under conditions of 2000 mg L<sup>-1</sup> N-NH₄ and extreme pH levels of 3 and 10. <em>Chlorolobion braunii</em> demonstrated the highest productivity, achieving a removal of 67.73 % of N-NH₄ and 30.59 % of N-NO₃, and reached the highest cellular density under extreme ammonium conditions, being the only strain capable of growing at acidic pH. Conversely, <em>Micractinium</em> sp. exhibited the highest growth under alkaline conditions. These results highlight the promising potential of native microalgae from pig effluents for wastewater remediation and their adaptation to environmental conditions.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00883"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality by design for transient RBD-Fc fusion protein production in Chinese hamster ovary cells 中国仓鼠卵巢细胞瞬时生成RBD-Fc融合蛋白的设计质量

Biotechnology Reports Pub Date : 2025-02-09 DOI: 10.1016/j.btre.2025.e00882

Araya Jivapetthai , Wanatchaporn Arunmanee , Natapol Pornputtapong

引用次数: 0

Integration of phytochemical profiling and computational approaches to evaluate the neuroprotective potential of Nardostachys jatamansi in Alzheimer's disease 整合植物化学分析和计算方法评估阿兹海默氏病的神经保护潜力

Biotechnology Reports Pub Date : 2025-02-08 DOI: 10.1016/j.btre.2025.e00881

Abdul Jalil Shah , Mohammad Younis Dar , Mohd Adnan , Tanmaykumar Varma , Dhairiya Agarwal , Prabha Garg , Reyaz Hassan Mir , Rampratap Meena , Mubashir Hussain Masoodi

{"title":"Integration of phytochemical profiling and computational approaches to evaluate the neuroprotective potential of Nardostachys jatamansi in Alzheimer's disease","authors":"Abdul Jalil Shah , Mohammad Younis Dar , Mohd Adnan , Tanmaykumar Varma , Dhairiya Agarwal , Prabha Garg , Reyaz Hassan Mir , Rampratap Meena , Mubashir Hussain Masoodi","doi":"10.1016/j.btre.2025.e00881","DOIUrl":"10.1016/j.btre.2025.e00881","url":null,"abstract":"<div><div>Despite broad spectrum utility of <em>Nardostachys jatamansi</em> (D. Don) DC, little is known about the molecular processes that underlie its anti-Alzheimer action. To investigate the molecular targets and therapeutic potential of <em>N. jatamansi</em> for Alzheimer's disease (AD), we used Gas Chromatography-Mass Spectrometry (GC-MS), ADMET analysis, network pharmacology, differential gene expression analysis, molecular docking, and molecular dynamics (MD) simulations. The STITCH database was used for network creation and protein-protein interaction analysis, while Cytoscape was used for network visualization and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and Gene Ontology (GO) for term enrichment. Additionally, to investigate the intermolecular interactions between the active chemicals and target proteins, molecular docking experiments were conducted using the Blind docking on the Achilles server. The stability of the PS1 gene complex with Spirojatamol, was further evaluated using MD simulations. With Spirojatamol showing the highest binding energy scores against PS1 (−6.9 kcal/mol), molecular docking confirmed the activity of this metabolite against AD targets PS1 and Spirojatamol formed a stable complex at 100 nanoseconds, according to additional investigation using MD simulations. Significant ligand-protein interactions were verified by binding free energy calculations using the MM/GBSA technique. The PS1-Spirojatamol complex had a binding energy of ΔG: −36.95 ± 5.00 kcal/mol. By focusing on several genes and pathways, involved in AD, this work reveals the molecular underpinnings behind <em>N. jatamansi</em> possible use in the treatment of AD.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00881"},"PeriodicalIF":0.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salinity induced changes in esterase, peroxidase and alcohol dehydrogenase isozymes and leaf soluble proteins in salinity susceptible and salinity tolerant sugarcane genotypes 盐度诱导盐敏感型和耐盐型甘蔗酯酶、过氧化物酶和乙醇脱氢酶同工酶及叶片可溶性蛋白的变化

Biotechnology Reports Pub Date : 2025-02-05 DOI: 10.1016/j.btre.2025.e00880

Manisha Rameshrao Patil , A.A. Kale , Ajay Kumar Singh , Priyanka Rameshrao Patil , Shaheen Badshah Inamdar , R.D. Satbhai

{"title":"Salinity induced changes in esterase, peroxidase and alcohol dehydrogenase isozymes and leaf soluble proteins in salinity susceptible and salinity tolerant sugarcane genotypes","authors":"Manisha Rameshrao Patil , A.A. Kale , Ajay Kumar Singh , Priyanka Rameshrao Patil , Shaheen Badshah Inamdar , R.D. Satbhai","doi":"10.1016/j.btre.2025.e00880","DOIUrl":"10.1016/j.btre.2025.e00880","url":null,"abstract":"<div><div>The salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 were evaluated for Peroxidase (POX), Esterase (EST) and Alcohol Dehydrogenase (ADH) isozymes and soluble protein profiling by SDS and native-PAGE at salinity levels 0.41 dSm<sup>-1</sup>, 2.31 dSm<sup>-1</sup>, 4.21 dSm<sup>-1</sup>, and 8.01 dSm<sup>-1</sup> maintained by NaCl solution. The plant height, number of leaves and seedling diameter got reduced in salinity susceptible sugarcane genotype CoC-671 as well as salinity tolerant sugarcane genotype CoM-265 with increase in salinity levels. However, reduction in plant height, number of leaves and seedling diameter was less in salinity tolerant sugarcane genotype CoM-265 as compared to salinity susceptible sugarcane genotype CoC-671. The POX isozyme profiling revealed that salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 had variation in soluble protein band intensity at different salinity levels with relative mobility (Rm) 0.137. The present study could be useful for genetic variability analysis in sugarcane genotypes differing in salinity stress tolerance capability.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00880"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and purification of a novel bacteriophage T7 endonuclease from the Kogelberg Biosphere Reserve (KBR) biodiversity hotspot 来自Kogelberg生物圈保护区（KBR）生物多样性热点的新型噬菌体T7内切酶的鉴定和纯化

Biotechnology Reports Pub Date : 2025-01-23 DOI: 10.1016/j.btre.2025.e00877

Priyen Pillay , Maabo Moralo , Sibongile Mtimka , Taola Shai , Kirsty Botha , Lusisizwe Kwezi , Tsepo L. Tsekoa

{"title":"Identification and purification of a novel bacteriophage T7 endonuclease from the Kogelberg Biosphere Reserve (KBR) biodiversity hotspot","authors":"Priyen Pillay , Maabo Moralo , Sibongile Mtimka , Taola Shai , Kirsty Botha , Lusisizwe Kwezi , Tsepo L. Tsekoa","doi":"10.1016/j.btre.2025.e00877","DOIUrl":"10.1016/j.btre.2025.e00877","url":null,"abstract":"<div><div>The four-way (Holliday) DNA junction is a key intermediate in homologous recombination, a ubiquitous process that is important in DNA repair and generation of genetic diversity. The final stages of recombination require resolution of the junction into nicked-duplex species by the action of a junction-resolving enzyme. The enzymes involved are nucleases that are highly selective for the structure of branched DNA. Here we present the isolation, expression and purification of a novel T7 endonuclease from the Kogelberg Biosphere Reserve (KBR), which possesses junction resolving capabilities. An initial approach was employed where the process was scaled up to 3 L with IPTG concentration of 0.1 mM at 30 °C and purified via immobilised metal affinity chromatography (IMAC). Expression titres of 20 ± 0.003 µg.L<sup>-1</sup> culture were achieved with the amount of KBR-T7 endonuclease required per reaction ranging from as low as 10 to 100 nanograms. The solubility of the enzyme was relatively poor; however, enzyme activity was not affected. A derivative for improved solubility and efficacy was then designed from this original wild-type version, MBP-KBR-T7 and was expressed under similar conditions at 20 °C yielding 1.63 ± 0.154 mg.L<sup>-1</sup> of formulated enzyme. This novel high value enzyme derivative is a valuable asset within the molecular reagent space as a tool for confirming both <em>in vivo</em> and <em>in vitro</em> genome editing; therefore, a means to produce it recombinantly in a scalable and technoeconomicaly viable process is highly desirable.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00877"},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of bromoform by Curvularia fungi provides a natural pathway to mitigate enteric methane emissions from ruminants 曲霉菌生物合成溴仿为减少反刍动物肠道甲烷排放提供了一条天然途径

Biotechnology Reports Pub Date : 2025-01-14 DOI: 10.1016/j.btre.2025.e00876

Thomas Loan , Avinash Karpe , Saeid Babaei , Stuart Denman , Chunghong Chen , Matthias Joust , Kristy Lam , Dawar Hussain , Sapna Pillai Vibhakaran , Matthew Callaghan , Abed Chaudhury , Karen Paco , Nigel Tomkins , Tristan Yang , Stephanie Payne , Michael Ayliffe , Ming Luo

{"title":"Biosynthesis of bromoform by Curvularia fungi provides a natural pathway to mitigate enteric methane emissions from ruminants","authors":"Thomas Loan , Avinash Karpe , Saeid Babaei , Stuart Denman , Chunghong Chen , Matthias Joust , Kristy Lam , Dawar Hussain , Sapna Pillai Vibhakaran , Matthew Callaghan , Abed Chaudhury , Karen Paco , Nigel Tomkins , Tristan Yang , Stephanie Payne , Michael Ayliffe , Ming Luo","doi":"10.1016/j.btre.2025.e00876","DOIUrl":"10.1016/j.btre.2025.e00876","url":null,"abstract":"<div><div>The ruminant livestock industry is the largest agricultural emissions source of anthropogenic greenhouse gases, primarily from enteric methane. Several technologies, both natural and synthetic are being investigated to mitigate enteric methane emissions. Macroalgae derived feed ingredients that contain bromoform, a recognised inhibitor of methanogenesis, are an effective natural approach for methane mitigation. However, producing sufficient biomass economically to satisfy the livestock industry at a global scale is challenging. Here we demonstrate that a <em>Curvularia</em> soil fungi isolate can be cultured to produce bromoform and subsequently inhibit methanogenesis in pure cultures of <em>Methanobrevibacter smithii</em> and in mixed cultures of ovine rumen fluid. This highly culturable fungal species produces no known toxins and creates an exciting new, scalable and natural alternative for reducing ruminant livestock emissions.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00876"},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0