Biotechnology Reports最新文献

{"title":"In-situ partial reduction of biochar by overlaying a syngas stream","authors":"Valentin Chataigner ,&nbsp;Dominique Tarlet ,&nbsp;François Ricoul ,&nbsp;Jérôme Bellettre","doi":"10.1016/j.btre.2025.e00892","DOIUrl":"10.1016/j.btre.2025.e00892","url":null,"abstract":"<div><div>This work focuses on the partial reduction of a hot biochar by a hot syngas coming out of the partial oxidation zone in the Ariane® pyrolysis prototype. The patented geometry of this pyrolyser, with separate pyrolysis and partial oxidation zones, enables easy management of biochar and syngas flows. The aim is to increase the specific surface area of the biochar to achieve more agronomic specifications, while maintaining a high carbon content in the biochar. The experimental implementation of this partial reduction is achieved by adding a partial reduction zone at the bottom of the reactor, enabling direct contact between biochar and syngas. This part now enables biochar and syngas streams to be mixed in a high-temperature zone (<span><math><mrow><mo>&gt;</mo><msup><mn>500</mn><mo>∘</mo></msup><mi>C</mi></mrow></math></span>). The addition of this part increased the specific surface area of the biochar by <span><math><mrow><mn>250</mn><mspace></mspace><msup><mrow><mi>m</mi></mrow><mn>2</mn></msup><mo>/</mo><mi>g</mi></mrow></math></span> and preserved the carbon content of the biochar regardless of the biomass flow rate. Around <span><math><mrow><mn>20</mn><mspace></mspace><mo>%</mo></mrow></math></span> of the carbon in the biochar is consumed by the reduction and transferred from the biochar to the syngas. The syngas is thus enriched in particular in <span><math><msub><mi>H</mi><mn>2</mn></msub></math></span> and <span><math><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></math></span> and depleted in <span><math><mrow><mi>C</mi><msub><mi>H</mi><mn>4</mn></msub></mrow></math></span>, which opens up a wider range of development opportunities for this gas (biomethane, biohydrogen, biokerosene, cogeneration, etc.). Thanks to this activation method, a better compromise has been achieved (compared with the previous version of the prototype) between quality (specific surface area and carbon content) and quantity of biochar produced (anhydrous yield).</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00892"},"PeriodicalIF":0.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Montbretia flowers as a source of bioactive crocins: Biotechnology tools and delivery systems","authors":"Lucía Morote ,&nbsp;Cristian Martínez Martínez Fajardo ,&nbsp;María Mondéjar López ,&nbsp;Elena Moreno-Gimenez ,&nbsp;Ángela Rubio-Moraga ,&nbsp;Olivia Costantina Demurtas ,&nbsp;Gianfranco Diretto ,&nbsp;Enrique Niza ,&nbsp;Verónica Aragonés ,&nbsp;Alberto López Jiménez ,&nbsp;José-Antonio Daròs ,&nbsp;Oussama Ahrazem ,&nbsp;Lourdes Gómez-Gómez","doi":"10.1016/j.btre.2025.e00891","DOIUrl":"10.1016/j.btre.2025.e00891","url":null,"abstract":"<div><div>Crocins are potent antioxidants with significant therapeutic potential, exhibiting anti-inflammatory, anticancer, and antidepressant properties. The ornamental plant <em>Crocos-mia x crocosmiiflora</em> is widely cultivated for its aromatic and vibrant flowers. In this study, we identified unique crocins as the primary pigments responsible for the flower's coloration. These metabolites predominantly consist of molecules with eight glucose units, followed by crocins containing six and seven glucose units. To elucidate the mo-lecular mechanisms underlying crocin biosynthesis in <em>C. x crocosmiiflora</em>, tran-scriptomic analysis was performed to identify key carotenoid cleavage dioxygenase (CCD) genes. Using <em>Crocus sativus</em> CsCCD2L gene as a bait, we identified a CCD transcript from the transcriptome data. Phylogenetic analysis revealed that the identified CCD belongs to the CCD2 subfamily, and it was designated as CroCCD2. Functional characterization of CroCCD2 was carried out using bacterial expression systems and <em>Nicotiana benthamiana</em> plants with a virus-mediated expression system. These experiments demonstrated that CroCCD2 efficiently converts the precursor zeaxanthin into crocetin, a key intermediate in crocin biosynthesis. Furthermore, we investigated the bioactivity of crocins and discovered that their anti-inflammatory effects depend on their vehiculation within exosomes or liposomes. This suggests that the transport mechanism is critical for the biological activity of crocins. Our findings highlight the specialization of CCD subfamilies in monocots and dicots for crocin biosynthesis and provide evidence of the anti-inflammatory activity of exosome-transported crocins. This study establishes a foundation for further research into the metabolic network of crocins in <em>C. x crocosmiiflora</em> and suggests that the CroCCD2 gene could be introduced into other crop plants to produce these bioactive apocarotenoids.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00891"},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cleaner horizons: Exploring advanced technologies for pollution remediation","authors":"Khadija Malik,&nbsp;Ashja Iftikhar,&nbsp;Quratulain Maqsood,&nbsp;Muhammad Rizwan Tariq,&nbsp;Shinawar Waseem Ali","doi":"10.1016/j.btre.2025.e00890","DOIUrl":"10.1016/j.btre.2025.e00890","url":null,"abstract":"<div><div>Soil pollution causes many harmful effects by its contaminants or pollutants, which are known as soil pollutants. They are causing serious problems in plants as well as in humans. By entering into plants, harmful chemicals become part of the food chain. When humans consume contaminated food, it has harmful effects on human health. Pollutants are making soil unfit for living. Many techniques are being used for the remediation of soil pollution. Some are traditional techniques; some are innovative and effective as emerging science and technology are going on. In this review, we have discussed some significant methods, their aspects, and how they are playing their role in the remediation. Biological methods such as living organisms, chemical, and genetic manipulation are modern techniques that are being used for soil pollution remediation. Genetic manipulations sometimes change the enzyme processes, which enhance the whole activity by changing some of the proteins of organisms related to enzymes. Pollution remediation can be done by the process of bio-augmentation, which uses different types of strains of microbes for treatment. As there is an increase in the formation of OH compounds, advanced oxidation technologies are being introduced to treat them. Trace metals and heavy metals are also a big problem for soil pollution, which can be treated by phytoremediation techniques that use many different strategies. Nanoparticles are also being used for the treatment of compounds like nitrates, manganese, arsenic, etc. This review will guide you through the different technologies for soil pollution remediation.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00890"},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized expression of human interleukin-15 in Nicotiana benthamiana and in vitro assessment of its activity on human keratinocytes","authors":"Chalatorn Charnsatabut ,&nbsp;Pipob Suwanchaikasem ,&nbsp;Kaewta Rattanapisit ,&nbsp;Iksen Iksen ,&nbsp;Varisa Pongrakhananon ,&nbsp;Christine Joy I. Bulaon ,&nbsp;Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2025.e00889","DOIUrl":"10.1016/j.btre.2025.e00889","url":null,"abstract":"<div><div>Human interleukin-15 (hIL-15) is a cytokine essential for immune modulation with therapeutic applications in cancer and chronic wound healing. Although hIL-15 is commercially available, large-scale production studies remain limited. With promising clinical trial results, demand for hIL-15 is expected to rise. Plant expression systems offer a sustainable, low-cost alternative for rapid biopharmaceutical production. In this study, we optimized hIL-15 expression in <em>Nicotiana benthamiana</em> and assessed its physicochemical properties and biological activity. We fused hIL-15 to the Fc domain of human IgG1 for efficient purification. Through optimization of the pre- and post-infiltration conditions, we achieved transient expression and recovery at 4 dpi, yielding 33.8 µg/g fresh weight. Peptide mapping confirmed 97 % overall sequence coverage of the primary structure. Treatment with plant-produced hIL-15-Fc effectively promoted human keratinocyte HaCaT cell proliferation and migration <em>in vitro</em>. These findings demonstrated the potential of plant-based platforms for producing therapeutic recombinant hIL-15 that support wound healing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00889"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical-induced variations in callus regenerated Atropa acuminata plants: A study on Ethyl Methanesulfonate effects","authors":"Shabeer Ahmad Dar ,&nbsp;Ishfaq Shafi Khan ,&nbsp;Gousia Majeed ,&nbsp;Sumira Tyub ,&nbsp;Irshad Ahmad Nawchoo ,&nbsp;Azra Nahaid Kamili","doi":"10.1016/j.btre.2025.e00888","DOIUrl":"10.1016/j.btre.2025.e00888","url":null,"abstract":"<div><div><em>Atropa acuminata</em> Royle ex Lindl. a member of the solanaceae family is a critically endangered medicinal herb indigenous to the Himalayan region of Kashmir and revered for its therapeutic and economic significance. Ethyl Methanesulfonate (EMS), a chemical mutagen, is commonly used to induce mutations for plant breeding and research purposes. In this study, callus cultures derived from leaf explants of <em>A. acuminata</em> were cultivated on Murashige and Skoog (MS) medium supplemented with Thidiazuron (0.1 mg/l) and treated with varying concentrations of EMS (0.1%, 1.0%) over defined time intervals. The impact of EMS concentrations and exposure durations, as well as their interactions, on the characteristics of regenerated callus were rigorously examined. The proliferation of shoots exhibited a diminishing trend with escalating EMS concentrations. Additionally, EMS elicited substantial alterations in the morphological and physiological attributes of the regenerated plants. Among the fifteen traits evaluated, mutant lines designated as M10, M11, and M6 displayed the most pronounced variations in morphological and physiological parameters compared to the control. Notably, the mutant line M10 exhibited significantly elevated shoot dry weight, root fresh weight, chlorophyll, and carotenoid contents demonstrating up to 98%, 31%, 348%, and 642% enhancements respectively as compared to the control. These mutant lines hold promise for further exploitation in augmenting physiological characteristics and enhancing the yield and quality of terpene glycosides in <em>A. acuminata</em>.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00888"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a GFP reporter for splicing analysis in mammalian cells","authors":"Arthur T. Menezes,&nbsp;Helder Y. Nagasse,&nbsp;Hilan R. M. Lopes,&nbsp;Patricia P. Coltri","doi":"10.1016/j.btre.2025.e00887","DOIUrl":"10.1016/j.btre.2025.e00887","url":null,"abstract":"<div><div>Eukaryotic genes are formed by exons and introns. Pre-mRNA splicing promotes exon ligation and intron removal and is performed by a specialized macromolecular machinery named spliceosome, composed of five small ribonucleoprotein particles (snRNPs) and more than one hundred proteins. The activity of this complex is highly accurate due to the coordinated activity of its components. Altered splicing has been related to the development of several diseases, including neurodegenerative disorders, such as amyotrophic lateral sclerosis, and different types of cancer. Detailed understanding of splicing regulation in eukaryotic cells can be achieved using splicing reporter systems. We designed a reporter plasmid suitable for splicing analysis in cultured mammalian cells. Our reporter is based on GFP expression, and the splicing outcome can be easily visualized by fluorescence microscopy. We quantified splicing activity in two human cell lines, HEK-293T and MDA-MB-231, confirming its suitability for use in live cells in culture.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00887"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the multifaceted roles of resuscitation-promoting factors in tuberculosis: Implications for diagnosis, vaccine development, and drug targeting","authors":"Gamze Tanriver ,&nbsp;Salman Ali Khan ,&nbsp;Artur Góra ,&nbsp;Novel N Chegou ,&nbsp;Shima Mahmoudi","doi":"10.1016/j.btre.2025.e00886","DOIUrl":"10.1016/j.btre.2025.e00886","url":null,"abstract":"<div><div>Tuberculosis (TB) remains a significant global health challenge, necessitating continued research efforts to unravel its complex pathogenesis and advance diagnostic, therapeutic, and preventive strategies. Resuscitation-promoting factors (Rpfs) are peptidoglycan-hydrolyzing enzymes that have gained attention due to their key roles in TB infection dynamics. This review aims to provide a comprehensive overview of Rpfs in TB, highlighting their immunological roles, diagnostic potential, and implications for vaccine development and drug targeting through both in silico and experimental approaches. Rpfs exhibit diverse roles in TB, influencing bacterial resuscitation from dormancy, and immune modulation through interactions with host immune cells, such as dendritic cells, and they are potential targets for novel diagnostic and therapeutic interventions. Their ability to stimulate immune responses, particularly interferon-γ production by T cells, underscores their potential as vaccine candidates against TB. Moreover, Rpfs represent promising targets for drug discovery, with inhibitors potentially disrupting bacterial resuscitation and growth.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00886"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells","authors":"Teresa Franchi-Mendes ,&nbsp;Marília Silva ,&nbsp;Maria Catarina Carreira ,&nbsp;Ana Luísa Cartaxo ,&nbsp;Paulo Vale ,&nbsp;Panagiotis Karakaidos ,&nbsp;Apostolos Klinakis ,&nbsp;Ana Fernandes-Platzgummer ,&nbsp;Cláudia L. da Silva","doi":"10.1016/j.btre.2025.e00878","DOIUrl":"10.1016/j.btre.2025.e00878","url":null,"abstract":"<div><h3>Introduction</h3><div>Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers.</div></div><div><h3>Materials &amp; Methods</h3><div>A gamma-irradiated human platelet lysate (HPL) supplement was used to develop a xeno(geneic)-free process for the isolation and scalable expansion of human bladder-derived SMC.</div></div><div><h3>Results</h3><div>Cells were isolated using an explant-based technique and expanded ex vivo, expressing typical SMC markers (α-SMA, desmin, caldesmon and SM22-α). Cell culture was successfully scaled-up using spinner flasks combined with plastic microcarriers, starting with a 2.8 × 10³ cells/cm² inoculum (i.e. 1 × 10⁶ cells). Cell-microcarrier adhesion rates exceeded 80% within 24 hours with fold expansion ranging from 3.5 to 16.8 after 7 days, dependent on donor variability. Harvested cells retained their SMC phenotype.</div></div><div><h3>Conclusions</h3><div>This xeno-free, GMP compliant process enables scalable manufacturing of human bladder-derived SMC while preserving cell identity, potentially advancing clinical applications in bladder engineering.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00878"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding black soldier fly (BSF; Hermetia illucens; Diptera: Stratiomyidae) in the developing world: Use of BSF larvae as a biological tool to recycle various organic biowastes for alternative protein production in Nepal","authors":"Bhola Gautam ,&nbsp;Sundar Tiwari ,&nbsp;Min Raj Pokhrel ,&nbsp;Jeffery K. Tomberlin ,&nbsp;Prabhat Khanal","doi":"10.1016/j.btre.2025.e00879","DOIUrl":"10.1016/j.btre.2025.e00879","url":null,"abstract":"<div><div>The growing global demand for food, particularly animal protein, is intensifying challenges related to food security and environmental sustainability. The increase in organic waste generation, coupled with inefficient waste management, is further deteriorating living conditions by negatively impacting the environment and public health, especially in developing nations. This study investigated the potential of black soldier fly larvae (BSFL) to recycle major daily organic waste fractions in Nepal. BSFL were exposed to seven different biowaste-based substrates locally sourced from fruit and vegetable markets, farms, and food industries. Additionally, the study evaluated rapeseed cake as a supplement to enhance BSFL growth and nutritional content. BSFL survival rates exceeded 80 % on food industry waste but dropped to 63 % on high-moisture substrates like vegetable waste. Mixed vegetable waste (14.7 mg/day) and bakery waste (11.5 mg/day) supported higher average daily weight gain, likely due to their better nutrient values (soluble carbohydrates and proteins). Although rapeseed cake alone hindered larval growth, its supplementation to biowastes improved growth, survival, and bioconversion rates, increased larval protein content up to 32 %, and reduced fat by 36 %. These findings indicate BSFL can effectively recycle diverse, locally available organic wastes in developing countries like Nepal, providing a sustainable source of domestic protein and contributing to feed security. As this is the first BSFL study in Nepal, further research is needed to elucidate the chemical and microbial safety of BSFL reared on biowastes and to develop technical solutions for commercial BSFL production in countries with a low-income economy.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00879"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae strains isolated from piggery wastewater in Ecuador: Effective nitrogen compound removal and growth potential in extremophile conditions","authors":"Karla Flores-Zambrano ,&nbsp;Wilson Tapia ,&nbsp;Pablo Castillejo","doi":"10.1016/j.btre.2025.e00883","DOIUrl":"10.1016/j.btre.2025.e00883","url":null,"abstract":"<div><div>Effluents generated by anthropogenic activities are a significant source of pollution and eutrophication in natural water bodies. In Ecuador, the increase in pig production has exacerbated this issue due to the untreated discharge of pig effluents. This study focused on the characterization of native microalgae present in pig effluents and the evaluation of their capacity to remove nitrogenous compounds under various conditions, with the aim of identifying efficient strains for phycoremediation. Four microalgal strains were isolated and molecularly identified as <em>Radiococcus polycoccus, Chlorolobion braunii, Micractinium</em> sp., and <em>Desmodesmus multivariabilis</em>. The cultures were exposed to initial concentrations of 100 mg L^-1 N-NH₄ and 49.97 mg L^-1 N-NO₃ for 12 days to assess their cellular growth and nutrient removal rates. Growth kinetics were analyzed under conditions of 2000 mg L^-1 N-NH₄ and extreme pH levels of 3 and 10. <em>Chlorolobion braunii</em> demonstrated the highest productivity, achieving a removal of 67.73 % of N-NH₄ and 30.59 % of N-NO₃, and reached the highest cellular density under extreme ammonium conditions, being the only strain capable of growing at acidic pH. Conversely, <em>Micractinium</em> sp. exhibited the highest growth under alkaline conditions. These results highlight the promising potential of native microalgae from pig effluents for wastewater remediation and their adaptation to environmental conditions.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00883"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}