Biotechnology Reports最新文献_第2页

Cleaner horizons: Exploring advanced technologies for pollution remediation 更清洁的视野：探索先进的污染修复技术

Biotechnology Reports Pub Date : 2025-03-28 DOI: 10.1016/j.btre.2025.e00890

Khadija Malik, Ashja Iftikhar, Quratulain Maqsood, Muhammad Rizwan Tariq, Shinawar Waseem Ali

{"title":"Cleaner horizons: Exploring advanced technologies for pollution remediation","authors":"Khadija Malik, Ashja Iftikhar, Quratulain Maqsood, Muhammad Rizwan Tariq, Shinawar Waseem Ali","doi":"10.1016/j.btre.2025.e00890","DOIUrl":"10.1016/j.btre.2025.e00890","url":null,"abstract":"<div><div>Soil pollution causes many harmful effects by its contaminants or pollutants, which are known as soil pollutants. They are causing serious problems in plants as well as in humans. By entering into plants, harmful chemicals become part of the food chain. When humans consume contaminated food, it has harmful effects on human health. Pollutants are making soil unfit for living. Many techniques are being used for the remediation of soil pollution. Some are traditional techniques; some are innovative and effective as emerging science and technology are going on. In this review, we have discussed some significant methods, their aspects, and how they are playing their role in the remediation. Biological methods such as living organisms, chemical, and genetic manipulation are modern techniques that are being used for soil pollution remediation. Genetic manipulations sometimes change the enzyme processes, which enhance the whole activity by changing some of the proteins of organisms related to enzymes. Pollution remediation can be done by the process of bio-augmentation, which uses different types of strains of microbes for treatment. As there is an increase in the formation of OH compounds, advanced oxidation technologies are being introduced to treat them. Trace metals and heavy metals are also a big problem for soil pollution, which can be treated by phytoremediation techniques that use many different strategies. Nanoparticles are also being used for the treatment of compounds like nitrates, manganese, arsenic, etc. This review will guide you through the different technologies for soil pollution remediation.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00890"},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized expression of human interleukin-15 in Nicotiana benthamiana and in vitro assessment of its activity on human keratinocytes 人白细胞介素-15在烟叶中的优化表达及其对人角质形成细胞活性的体外评价

Biotechnology Reports Pub Date : 2025-03-25 DOI: 10.1016/j.btre.2025.e00889

Chalatorn Charnsatabut , Pipob Suwanchaikasem , Kaewta Rattanapisit , Iksen Iksen , Varisa Pongrakhananon , Christine Joy I. Bulaon , Waranyoo Phoolcharoen

{"title":"Optimized expression of human interleukin-15 in Nicotiana benthamiana and in vitro assessment of its activity on human keratinocytes","authors":"Chalatorn Charnsatabut , Pipob Suwanchaikasem , Kaewta Rattanapisit , Iksen Iksen , Varisa Pongrakhananon , Christine Joy I. Bulaon , Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2025.e00889","DOIUrl":"10.1016/j.btre.2025.e00889","url":null,"abstract":"<div><div>Human interleukin-15 (hIL-15) is a cytokine essential for immune modulation with therapeutic applications in cancer and chronic wound healing. Although hIL-15 is commercially available, large-scale production studies remain limited. With promising clinical trial results, demand for hIL-15 is expected to rise. Plant expression systems offer a sustainable, low-cost alternative for rapid biopharmaceutical production. In this study, we optimized hIL-15 expression in <em>Nicotiana benthamiana</em> and assessed its physicochemical properties and biological activity. We fused hIL-15 to the Fc domain of human IgG1 for efficient purification. Through optimization of the pre- and post-infiltration conditions, we achieved transient expression and recovery at 4 dpi, yielding 33.8 µg/g fresh weight. Peptide mapping confirmed 97 % overall sequence coverage of the primary structure. Treatment with plant-produced hIL-15-Fc effectively promoted human keratinocyte HaCaT cell proliferation and migration <em>in vitro</em>. These findings demonstrated the potential of plant-based platforms for producing therapeutic recombinant hIL-15 that support wound healing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00889"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical-induced variations in callus regenerated Atropa acuminata plants: A study on Ethyl Methanesulfonate effects 化学诱导的刺蒺藜愈伤组织变异：甲磺酸乙酯效应的研究

Biotechnology Reports Pub Date : 2025-03-21 DOI: 10.1016/j.btre.2025.e00888

Shabeer Ahmad Dar , Ishfaq Shafi Khan , Gousia Majeed , Sumira Tyub , Irshad Ahmad Nawchoo , Azra Nahaid Kamili

{"title":"Chemical-induced variations in callus regenerated Atropa acuminata plants: A study on Ethyl Methanesulfonate effects","authors":"Shabeer Ahmad Dar , Ishfaq Shafi Khan , Gousia Majeed , Sumira Tyub , Irshad Ahmad Nawchoo , Azra Nahaid Kamili","doi":"10.1016/j.btre.2025.e00888","DOIUrl":"10.1016/j.btre.2025.e00888","url":null,"abstract":"<div><div><em>Atropa acuminata</em> Royle ex Lindl. a member of the solanaceae family is a critically endangered medicinal herb indigenous to the Himalayan region of Kashmir and revered for its therapeutic and economic significance. Ethyl Methanesulfonate (EMS), a chemical mutagen, is commonly used to induce mutations for plant breeding and research purposes. In this study, callus cultures derived from leaf explants of <em>A. acuminata</em> were cultivated on Murashige and Skoog (MS) medium supplemented with Thidiazuron (0.1 mg/l) and treated with varying concentrations of EMS (0.1%, 1.0%) over defined time intervals. The impact of EMS concentrations and exposure durations, as well as their interactions, on the characteristics of regenerated callus were rigorously examined. The proliferation of shoots exhibited a diminishing trend with escalating EMS concentrations. Additionally, EMS elicited substantial alterations in the morphological and physiological attributes of the regenerated plants. Among the fifteen traits evaluated, mutant lines designated as M10, M11, and M6 displayed the most pronounced variations in morphological and physiological parameters compared to the control. Notably, the mutant line M10 exhibited significantly elevated shoot dry weight, root fresh weight, chlorophyll, and carotenoid contents demonstrating up to 98%, 31%, 348%, and 642% enhancements respectively as compared to the control. These mutant lines hold promise for further exploitation in augmenting physiological characteristics and enhancing the yield and quality of terpene glycosides in <em>A. acuminata</em>.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00888"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a GFP reporter for splicing analysis in mammalian cells 用于哺乳动物细胞剪接分析的GFP报告基因的设计

Biotechnology Reports Pub Date : 2025-03-13 DOI: 10.1016/j.btre.2025.e00887

Arthur T. Menezes, Helder Y. Nagasse, Hilan R. M. Lopes, Patricia P. Coltri

引用次数: 0

Exploring the multifaceted roles of resuscitation-promoting factors in tuberculosis: Implications for diagnosis, vaccine development, and drug targeting 探讨复苏促进因素在结核病中的多方面作用：对诊断、疫苗开发和药物靶向的影响

Biotechnology Reports Pub Date : 2025-03-13 DOI: 10.1016/j.btre.2025.e00886

Gamze Tanriver , Salman Ali Khan , Artur Góra , Novel N Chegou , Shima Mahmoudi

{"title":"Exploring the multifaceted roles of resuscitation-promoting factors in tuberculosis: Implications for diagnosis, vaccine development, and drug targeting","authors":"Gamze Tanriver , Salman Ali Khan , Artur Góra , Novel N Chegou , Shima Mahmoudi","doi":"10.1016/j.btre.2025.e00886","DOIUrl":"10.1016/j.btre.2025.e00886","url":null,"abstract":"<div><div>Tuberculosis (TB) remains a significant global health challenge, necessitating continued research efforts to unravel its complex pathogenesis and advance diagnostic, therapeutic, and preventive strategies. Resuscitation-promoting factors (Rpfs) are peptidoglycan-hydrolyzing enzymes that have gained attention due to their key roles in TB infection dynamics. This review aims to provide a comprehensive overview of Rpfs in TB, highlighting their immunological roles, diagnostic potential, and implications for vaccine development and drug targeting through both in silico and experimental approaches. Rpfs exhibit diverse roles in TB, influencing bacterial resuscitation from dormancy, and immune modulation through interactions with host immune cells, such as dendritic cells, and they are potential targets for novel diagnostic and therapeutic interventions. Their ability to stimulate immune responses, particularly interferon-γ production by T cells, underscores their potential as vaccine candidates against TB. Moreover, Rpfs represent promising targets for drug discovery, with inhibitors potentially disrupting bacterial resuscitation and growth.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00886"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells 用于分离和扩增人膀胱平滑肌细胞的无异种平台

Biotechnology Reports Pub Date : 2025-02-11 DOI: 10.1016/j.btre.2025.e00878

Teresa Franchi-Mendes , Marília Silva , Maria Catarina Carreira , Ana Luísa Cartaxo , Paulo Vale , Panagiotis Karakaidos , Apostolos Klinakis , Ana Fernandes-Platzgummer , Cláudia L. da Silva

{"title":"Xenogeneic-free platform for the isolation and scalable expansion of human bladder smooth muscle cells","authors":"Teresa Franchi-Mendes , Marília Silva , Maria Catarina Carreira , Ana Luísa Cartaxo , Paulo Vale , Panagiotis Karakaidos , Apostolos Klinakis , Ana Fernandes-Platzgummer , Cláudia L. da Silva","doi":"10.1016/j.btre.2025.e00878","DOIUrl":"10.1016/j.btre.2025.e00878","url":null,"abstract":"<div><h3>Introduction</h3><div>Smooth muscle cells (SMC) play a crucial role in bladder tissue engineering strategies. Scalable, Good Manufacturing Practice (GMP)-compliant platforms are essential for producing clinically relevant cell numbers.</div></div><div><h3>Materials & Methods</h3><div>A gamma-irradiated human platelet lysate (HPL) supplement was used to develop a xeno(geneic)-free process for the isolation and scalable expansion of human bladder-derived SMC.</div></div><div><h3>Results</h3><div>Cells were isolated using an explant-based technique and expanded ex vivo, expressing typical SMC markers (α-SMA, desmin, caldesmon and SM22-α). Cell culture was successfully scaled-up using spinner flasks combined with plastic microcarriers, starting with a 2.8 × 10<sup>3</sup> cells/cm<sup>2</sup> inoculum (i.e. 1 × 10<sup>6</sup> cells). Cell-microcarrier adhesion rates exceeded 80% within 24 hours with fold expansion ranging from 3.5 to 16.8 after 7 days, dependent on donor variability. Harvested cells retained their SMC phenotype.</div></div><div><h3>Conclusions</h3><div>This xeno-free, GMP compliant process enables scalable manufacturing of human bladder-derived SMC while preserving cell identity, potentially advancing clinical applications in bladder engineering.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00878"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding black soldier fly (BSF; Hermetia illucens; Diptera: Stratiomyidae) in the developing world: Use of BSF larvae as a biological tool to recycle various organic biowastes for alternative protein production in Nepal 扩大黑兵蝇(BSF；Hermetia illucens;双翅目：层蛾科)在发展中国家：在尼泊尔，利用BSF幼虫作为一种生物工具，回收各种有机生物废物，用于替代蛋白质生产

Biotechnology Reports Pub Date : 2025-02-10 DOI: 10.1016/j.btre.2025.e00879

Bhola Gautam , Sundar Tiwari , Min Raj Pokhrel , Jeffery K. Tomberlin , Prabhat Khanal

{"title":"Expanding black soldier fly (BSF; Hermetia illucens; Diptera: Stratiomyidae) in the developing world: Use of BSF larvae as a biological tool to recycle various organic biowastes for alternative protein production in Nepal","authors":"Bhola Gautam , Sundar Tiwari , Min Raj Pokhrel , Jeffery K. Tomberlin , Prabhat Khanal","doi":"10.1016/j.btre.2025.e00879","DOIUrl":"10.1016/j.btre.2025.e00879","url":null,"abstract":"<div><div>The growing global demand for food, particularly animal protein, is intensifying challenges related to food security and environmental sustainability. The increase in organic waste generation, coupled with inefficient waste management, is further deteriorating living conditions by negatively impacting the environment and public health, especially in developing nations. This study investigated the potential of black soldier fly larvae (BSFL) to recycle major daily organic waste fractions in Nepal. BSFL were exposed to seven different biowaste-based substrates locally sourced from fruit and vegetable markets, farms, and food industries. Additionally, the study evaluated rapeseed cake as a supplement to enhance BSFL growth and nutritional content. BSFL survival rates exceeded 80 % on food industry waste but dropped to 63 % on high-moisture substrates like vegetable waste. Mixed vegetable waste (14.7 mg/day) and bakery waste (11.5 mg/day) supported higher average daily weight gain, likely due to their better nutrient values (soluble carbohydrates and proteins). Although rapeseed cake alone hindered larval growth, its supplementation to biowastes improved growth, survival, and bioconversion rates, increased larval protein content up to 32 %, and reduced fat by 36 %. These findings indicate BSFL can effectively recycle diverse, locally available organic wastes in developing countries like Nepal, providing a sustainable source of domestic protein and contributing to feed security. As this is the first BSFL study in Nepal, further research is needed to elucidate the chemical and microbial safety of BSFL reared on biowastes and to develop technical solutions for commercial BSFL production in countries with a low-income economy.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00879"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae strains isolated from piggery wastewater in Ecuador: Effective nitrogen compound removal and growth potential in extremophile conditions 从厄瓜多尔养猪场废水中分离的微藻菌株：在极端微生物条件下有效去除氮化合物和生长潜力

Biotechnology Reports Pub Date : 2025-02-10 DOI: 10.1016/j.btre.2025.e00883

Karla Flores-Zambrano , Wilson Tapia , Pablo Castillejo

{"title":"Microalgae strains isolated from piggery wastewater in Ecuador: Effective nitrogen compound removal and growth potential in extremophile conditions","authors":"Karla Flores-Zambrano , Wilson Tapia , Pablo Castillejo","doi":"10.1016/j.btre.2025.e00883","DOIUrl":"10.1016/j.btre.2025.e00883","url":null,"abstract":"<div><div>Effluents generated by anthropogenic activities are a significant source of pollution and eutrophication in natural water bodies. In Ecuador, the increase in pig production has exacerbated this issue due to the untreated discharge of pig effluents. This study focused on the characterization of native microalgae present in pig effluents and the evaluation of their capacity to remove nitrogenous compounds under various conditions, with the aim of identifying efficient strains for phycoremediation. Four microalgal strains were isolated and molecularly identified as <em>Radiococcus polycoccus, Chlorolobion braunii, Micractinium</em> sp., and <em>Desmodesmus multivariabilis</em>. The cultures were exposed to initial concentrations of 100 mg L<sup>-1</sup> N-NH₄ and 49.97 mg L<sup>-1</sup> N-NO₃ for 12 days to assess their cellular growth and nutrient removal rates. Growth kinetics were analyzed under conditions of 2000 mg L<sup>-1</sup> N-NH₄ and extreme pH levels of 3 and 10. <em>Chlorolobion braunii</em> demonstrated the highest productivity, achieving a removal of 67.73 % of N-NH₄ and 30.59 % of N-NO₃, and reached the highest cellular density under extreme ammonium conditions, being the only strain capable of growing at acidic pH. Conversely, <em>Micractinium</em> sp. exhibited the highest growth under alkaline conditions. These results highlight the promising potential of native microalgae from pig effluents for wastewater remediation and their adaptation to environmental conditions.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00883"},"PeriodicalIF":0.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality by design for transient RBD-Fc fusion protein production in Chinese hamster ovary cells 中国仓鼠卵巢细胞瞬时生成RBD-Fc融合蛋白的设计质量

Biotechnology Reports Pub Date : 2025-02-09 DOI: 10.1016/j.btre.2025.e00882

Araya Jivapetthai , Wanatchaporn Arunmanee , Natapol Pornputtapong

引用次数: 0

Integration of phytochemical profiling and computational approaches to evaluate the neuroprotective potential of Nardostachys jatamansi in Alzheimer's disease 整合植物化学分析和计算方法评估阿兹海默氏病的神经保护潜力

Biotechnology Reports Pub Date : 2025-02-08 DOI: 10.1016/j.btre.2025.e00881

Abdul Jalil Shah , Mohammad Younis Dar , Mohd Adnan , Tanmaykumar Varma , Dhairiya Agarwal , Prabha Garg , Reyaz Hassan Mir , Rampratap Meena , Mubashir Hussain Masoodi

{"title":"Integration of phytochemical profiling and computational approaches to evaluate the neuroprotective potential of Nardostachys jatamansi in Alzheimer's disease","authors":"Abdul Jalil Shah , Mohammad Younis Dar , Mohd Adnan , Tanmaykumar Varma , Dhairiya Agarwal , Prabha Garg , Reyaz Hassan Mir , Rampratap Meena , Mubashir Hussain Masoodi","doi":"10.1016/j.btre.2025.e00881","DOIUrl":"10.1016/j.btre.2025.e00881","url":null,"abstract":"<div><div>Despite broad spectrum utility of <em>Nardostachys jatamansi</em> (D. Don) DC, little is known about the molecular processes that underlie its anti-Alzheimer action. To investigate the molecular targets and therapeutic potential of <em>N. jatamansi</em> for Alzheimer's disease (AD), we used Gas Chromatography-Mass Spectrometry (GC-MS), ADMET analysis, network pharmacology, differential gene expression analysis, molecular docking, and molecular dynamics (MD) simulations. The STITCH database was used for network creation and protein-protein interaction analysis, while Cytoscape was used for network visualization and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and Gene Ontology (GO) for term enrichment. Additionally, to investigate the intermolecular interactions between the active chemicals and target proteins, molecular docking experiments were conducted using the Blind docking on the Achilles server. The stability of the PS1 gene complex with Spirojatamol, was further evaluated using MD simulations. With Spirojatamol showing the highest binding energy scores against PS1 (−6.9 kcal/mol), molecular docking confirmed the activity of this metabolite against AD targets PS1 and Spirojatamol formed a stable complex at 100 nanoseconds, according to additional investigation using MD simulations. Significant ligand-protein interactions were verified by binding free energy calculations using the MM/GBSA technique. The PS1-Spirojatamol complex had a binding energy of ΔG: −36.95 ± 5.00 kcal/mol. By focusing on several genes and pathways, involved in AD, this work reveals the molecular underpinnings behind <em>N. jatamansi</em> possible use in the treatment of AD.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00881"},"PeriodicalIF":0.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0