Biotechnology Reports最新文献_第2页

An iterative approach to statistical optimization of exopolysaccharide produced by fermentation of Aureobasidium pullulans 普鲁兰毛霉发酵产胞外多糖的迭代统计优化方法

Biotechnology Reports Pub Date : 2025-08-15 DOI: 10.1016/j.btre.2025.e00914

Venessa Dsouza , Goldee Thoidingjam , Abdelrahman Saleh , Michael Zavrel

{"title":"An iterative approach to statistical optimization of exopolysaccharide produced by fermentation of Aureobasidium pullulans","authors":"Venessa Dsouza , Goldee Thoidingjam , Abdelrahman Saleh , Michael Zavrel","doi":"10.1016/j.btre.2025.e00914","DOIUrl":"10.1016/j.btre.2025.e00914","url":null,"abstract":"<div><div>Exopolysaccharides are biopolymers with wide-ranging industrial applications. To substitute fossil-based by bio-based, biodegradable polymers, exopolysaccharide production needs to become much more efficient. Pullulan, produced by <em>Aureobasidium pullulans</em>, is popular for its unique properties like film-formation, adhesiveness, biodegradability, etc. This study aims at demonstrating the optimization strategy on pullulan production using statistical optimization iteratively. The process followed a systematic approach including One Factor at A Time (OFAT) and Design Of Experiments (DOE). OFAT confirmed sucrose as carbon source, pH 6 and 4 days of incubation were optimum. Iterative Plackett-Burman Design (PBD) and Response Surface Methodology (RSM) further demonstrated interactions between significant media components and incubation conditions. The final pullulan titer was increased 6.34-fold to 113.5 ± 3.5 g/L of EPS, the highest titer achieved for this strain in shake flasks. Future investigations into fed-batch strategies and scale-up studies can be explored to further improve titers and reduce overall production costs.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00914"},"PeriodicalIF":0.0,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional properties of neodymium-doped hydroxyapatite nanoparticles for biomedical applications 生物医学用掺钕羟基磷灰石纳米颗粒的结构和功能特性

Biotechnology Reports Pub Date : 2025-08-15 DOI: 10.1016/j.btre.2025.e00916

K.Sai Manogna , K. Kusuma , G.Rajasekhara Reddy , B.Deva Prasad Raju , N.John Sushma

引用次数: 0

Improvement of protein quality and reduction of anti-nutritional factors in soybean meal by solid-state fermentation with Bacillus siamensis MH03 暹芽孢杆菌MH03固态发酵提高豆粕蛋白质品质和降低抗营养因子

Biotechnology Reports Pub Date : 2025-08-13 DOI: 10.1016/j.btre.2025.e00915

Tanawut Chotinu-kul, Gunjana Theeragool, Duenrut Chonudomkul

{"title":"Improvement of protein quality and reduction of anti-nutritional factors in soybean meal by solid-state fermentation with Bacillus siamensis MH03","authors":"Tanawut Chotinu-kul, Gunjana Theeragool, Duenrut Chonudomkul","doi":"10.1016/j.btre.2025.e00915","DOIUrl":"10.1016/j.btre.2025.e00915","url":null,"abstract":"<div><div>This study investigated the optimal conditions for enhancing protein quality and reducing anti-nutritional factors in soybean meal (SBM) through solid-state fermentation (SSF) with <em>Bacillus.</em> Among 78 isolates, strain MH03 exhibited the highest extracellular enzyme production and efficacy in enhancing SBM quality. Under optimized SSF conditions of 5 kg SBM, 5 cm thickness and 50 % initial moisture for 72 h, strain MH03 increased soluble protein from 3.31±0.21 to 10.16±0.27 g/100 g and significantly reduced anti-nutritional compounds, including glycinin, β-conglycinin, trypsin inhibitor, and oligosaccharides. Crude protein content increased from 47.89±0.07 % to 55.84±0.25 %, with protein solubility increased from 74.96±0.45 % to 90.54±0.28 %, with statistically significant increases. Other nutritional values were also significantly enhanced. Essential amino acids, such as valine, methionine, isoleucine, and lysine also increased. Based on 16S rRNA gene sequencing, MH03 was identified as <em>Bacillus siamensis</em>. These findings highlight the potential of <em>B. siamensis</em> MH03-fermented SBM as a high-quality protein ingredient for animal feed applications.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"48 ","pages":"Article e00915"},"PeriodicalIF":0.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green nanoscience for healthcare: Advancing biomedical innovation through eco-synthesized nanoparticle 绿色医疗纳米科学：通过生态合成纳米颗粒推进生物医学创新

Biotechnology Reports Pub Date : 2025-08-12 DOI: 10.1016/j.btre.2025.e00913

Anjuman Ayub , Atif Khurshid Wani , Suhaib Mohd Malik , Mehvish Ayub , Reena Singh , Chirag Chopra , Tabarak Malik

{"title":"Green nanoscience for healthcare: Advancing biomedical innovation through eco-synthesized nanoparticle","authors":"Anjuman Ayub , Atif Khurshid Wani , Suhaib Mohd Malik , Mehvish Ayub , Reena Singh , Chirag Chopra , Tabarak Malik","doi":"10.1016/j.btre.2025.e00913","DOIUrl":"10.1016/j.btre.2025.e00913","url":null,"abstract":"<div><div>Green synthesis is an eco-friendly and sustainable approach to nanoparticle production using biological sources such as plant extracts and microorganisms. Unlike traditional chemical methods, it aligns with green chemistry principles by reducing toxic reagents, minimizing waste, and lowering environmental impact. Green-synthesized nanoparticles have shown great potential, especially in biomedicine, for targeted drug delivery, antimicrobial treatments, and imaging applications. Their properties can be finely tuned by controlling size, shape, and composition, supporting applications across electronics, healthcare, and environmental engineering. Characterization techniques are essential for analyzing their structural and functional attributes. However, challenges remain in terms of scalability, reproducibility, and lack of standardized synthesis protocols. The aim of this review is to explore recent advances in green nanoparticle synthesis, evaluate key mechanisms, highlight biomedical and environmental applications, and discuss current limitations. The review also emphasizes future directions and the need for interdisciplinary collaboration to unlock the full potential of green nanotechnology.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00913"},"PeriodicalIF":0.0,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclodextrins in action: Modulating candida albicans biofilm formation and morphology 环糊精的作用：调节白色念珠菌生物膜的形成和形态

Biotechnology Reports Pub Date : 2025-08-09 DOI: 10.1016/j.btre.2025.e00912

Rita Márton , Hanna Hermann , Virág Tünde Kiss , Éva Fenyvesi , Lajos Szente , Mónika Molnár

{"title":"Cyclodextrins in action: Modulating candida albicans biofilm formation and morphology","authors":"Rita Márton , Hanna Hermann , Virág Tünde Kiss , Éva Fenyvesi , Lajos Szente , Mónika Molnár","doi":"10.1016/j.btre.2025.e00912","DOIUrl":"10.1016/j.btre.2025.e00912","url":null,"abstract":"<div><div>The primary objective of this research was to explore the bioactive potential of cyclodextrins in attenuating biofilm formation in <em>C. albicans</em>. The concentration-dependent effects of both native cyclodextrins and specific derivatives were studied at concentrations ranging from 0.1 to 12.5 mM, to determine the mechanisms by which the extent of biofilm formation can be reduced. Besides, the efficiency of various combinations of cyclodextrins and farnesol, as an antifungal substance, were examined.</div><div>The present study revealed both stimulatory and inhibitory effects of cyclodextrins on biofilm formation, depending on the structure and concentration. The bioactive potential of randomly methylated α- and γ-CD showed significant antifungal properties, as evidenced by a reduction in the biofilm formation. In addition, randomly methylated cyclodextrins were found to significantly enhance the antifungal activity of farnesol against <em>C. albicans</em>. Consequently, their synergistic effect may provide an excellent opportunity to produce a lower dose but more effective anticandidal formulation.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00912"},"PeriodicalIF":0.0,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seaweed-derived bioactives with anti-tyrosinase activity: a potential for skin-whitening cosmetics with in silico and in vitro approaches. 具有抗酪氨酸酶活性的海藻来源的生物活性物质：用硅和体外方法进行皮肤美白化妆品的潜力。

Biotechnology Reports Pub Date : 2025-08-07 eCollection Date: 2025-09-01 DOI: 10.1016/j.btre.2025.e00910

Arachaporn Thong-Olran, Supatchar Sermsakulwat, Tiwtawat Napiroon, Phuphiphat Jaikaew, Sumet Kongkiatpaiboon, Ngampuk Tayana, Bongkot Wichachucherd, Theppanya Charoenrat, Thrissawan Traijitt, Supenya Chittapun

{"title":"Seaweed-derived bioactives with anti-tyrosinase activity: a potential for skin-whitening cosmetics with <i>in silico</i> and <i>in vitro</i> approaches.","authors":"Arachaporn Thong-Olran, Supatchar Sermsakulwat, Tiwtawat Napiroon, Phuphiphat Jaikaew, Sumet Kongkiatpaiboon, Ngampuk Tayana, Bongkot Wichachucherd, Theppanya Charoenrat, Thrissawan Traijitt, Supenya Chittapun","doi":"10.1016/j.btre.2025.e00910","DOIUrl":"10.1016/j.btre.2025.e00910","url":null,"abstract":"<p><p>Seaweed is a promising source of bioactive compounds with potential applications in skincare, particularly for addressing hyperpigmentation. This study investigated the chemical profiles and biological activities of hydrophilic and lipophilic extracts from <i>Sargassum polycystum, Caulerpa lentillifera</i>, and <i>Gracilaria fisheri</i>. LC-MS and GC-MS analyses identified 8 and 112 compounds in hydrophilic and lipophilic extracts, respectively. Among these, <i>G. fisheri</i> lipophilic extracts exhibited the strongest anti-tyrosinase activity. Molecular docking identified eight compounds with low binding affinities comparable to kojic acid and tropolone, while ADMET predictions highlighted stigmasterol for its favorable skin permeability and safety. <i>In vitro</i> cytotoxicity assays confirmed its low toxicity (IC<sub>50</sub> = 3.38 ± 0.28 µg/mL) with no adverse effects at 0.006 µg/mL. Trypsin stability assays and molecular docking against serine proteases (trypsin and chymotrypsin) showed no significant off-target interactions. These findings suggest stigmasterol as a safe, selective, and effective natural tyrosinase inhibitor for potential use in skin-whitening formulation.</p>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"e00910"},"PeriodicalIF":0.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae: revolutionizing skin repair and enhancement. 微藻：革命性的皮肤修复和增强。

Biotechnology Reports Pub Date : 2025-08-06 eCollection Date: 2025-09-01 DOI: 10.1016/j.btre.2025.e00911

Negin Chinjoo, Abooali Golzary

{"title":"Microalgae: revolutionizing skin repair and enhancement.","authors":"Negin Chinjoo, Abooali Golzary","doi":"10.1016/j.btre.2025.e00911","DOIUrl":"10.1016/j.btre.2025.e00911","url":null,"abstract":"<p><p>Microalgae are attracting growing interest in the cosmetics industry due to their rich content of antioxidants, vitamins, and polyunsaturated fatty acids, which offer anti-aging, anti-inflammatory, and photoprotective benefits. Current research primarily focuses on individual species and isolated bioactive compounds. Several challenges persist, including scalability, cost-effective production, low biomass yields, and regulatory constraints. From an environmental perspective, microalgae can be sustainably cultivated on non-arable land, using recycled water and capturing CO₂, thus reducing carbon emissions. Technological advancements, such as photobioreactor systems, have further enhanced resource efficiency and minimized ecological impact. This review explores multiple microalgal species and their combined bioactive potential for innovative cosmetic formulations. By integrating green extraction techniques and circular economy principles, microalgae-based products can meet rising consumer demand for clean beauty while promoting sustainability. With increasing clinical evidence and ongoing technological progress, microalgae hold significant promise for driving eco-friendly innovation in the cosmetics sector.</p>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"e00911"},"PeriodicalIF":0.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prioritizing repurposable drugs for Alzheimer’s disease using network-based analysis with concurrent assessment of Long QT syndrome risk 利用基于网络的分析对阿尔茨海默病的可重复使用药物进行优先排序，同时评估长QT综合征的风险

Biotechnology Reports Pub Date : 2025-07-29 DOI: 10.1016/j.btre.2025.e00909

Alessio Funari , Enrico De Smaele , Paola Paci , Giulia Fiscon

{"title":"Prioritizing repurposable drugs for Alzheimer’s disease using network-based analysis with concurrent assessment of Long QT syndrome risk","authors":"Alessio Funari , Enrico De Smaele , Paola Paci , Giulia Fiscon","doi":"10.1016/j.btre.2025.e00909","DOIUrl":"10.1016/j.btre.2025.e00909","url":null,"abstract":"<div><div>Alzheimer's disease affects 6.9 million Americans aged 65 and older, a number expected to double by 2060. Eight FDA-approved drugs target Alzheimer's, but no cure is available, and most treatments are symptomatic. Drug repurposing, the use of FDA-approved drugs for new indications, is a promising strategy to address this lack of effective therapies. However, despite prior safety approval, repurposable drugs may still trigger unexpected side-effects in new contexts. This study introduces a network-based approach to minimize side-effect risk in drug repositioning, focusing on QT interval prolongation, a cardiac side-effect observed in Alzheimer's patients treated with acetylcholinesterase inhibitors. The method integrates Mode-of-Action and Random Walk with Restart analyses to identify repositioning candidates while assessing QT-related risk. This strategy identified promising compounds including acamprosate, tolcapone, sitagliptin, and diazoxide, with potential to mitigate disease pathology. Gene set enrichment analysis was used to computationally assess the compounds' ability to reverse disease-related gene expression signatures.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00909"},"PeriodicalIF":0.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of levan biosynthesis from Bacillus siamensis using batch and continuous stirred-tank bioreactors: A response surface methodology approach 间歇式和连续式搅拌槽生物反应器优化暹逻芽孢杆菌合成levan：响应面法

Biotechnology Reports Pub Date : 2025-07-29 DOI: 10.1016/j.btre.2025.e00908

Pongtorn Phengnoi , Nuttinee Teerakulkittipong , Kosin Teeparuksapun , Gary Antonio Lirio , Witawat Jangiam

{"title":"Optimization of levan biosynthesis from Bacillus siamensis using batch and continuous stirred-tank bioreactors: A response surface methodology approach","authors":"Pongtorn Phengnoi , Nuttinee Teerakulkittipong , Kosin Teeparuksapun , Gary Antonio Lirio , Witawat Jangiam","doi":"10.1016/j.btre.2025.e00908","DOIUrl":"10.1016/j.btre.2025.e00908","url":null,"abstract":"<div><div>Levan, a promising fructan polysaccharide for biopharmaceuticals, has limited large-scale production studies. This research optimized and scaled up levan biosynthesis from <em>Bacillus siamensis</em> in continuous stirred-tank bioreactors based on response surface methodology (RSM). Batch cultures optimized for 30 % (w/v) sucrose, pH 5.0, and 48 h incubation yielded a maximum 15.74 % (w/v) levan. The optimal batch conditions were evaluated in a continuous stirred-tank bioreactor, where dilution rates and mixing speeds were examined. At a dilution rate of 0.021 h⁻¹ and an agitation speed of 200 rpm, the maximum productivity was 17.96 % (w/v), and steady-state conditions were attained after three days of continuous fermentation. X-ray diffraction confirmed the amorphous nature of the levan, ideal for biomaterial applications. These results underline the potential of <em>B. siamensis</em> for high-yield levan production and provide a systematic approach for bioprocess parameter optimization, serving as a strong basis for its increased application in industrialized polysaccharide-based bioprocessing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00908"},"PeriodicalIF":0.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing cell characterization with microfluidics and AI: a comprehensive review of mechanical, electrical, and hybrid techniques 用微流体和人工智能增强细胞表征：机械、电气和混合技术的综合综述

Biotechnology Reports Pub Date : 2025-07-22 DOI: 10.1016/j.btre.2025.e00905

Marwan Taha , Anna-Maria Pappa , Hani Saleh , Anas Alazzam

引用次数: 0