Biotechnology Reports最新文献_第3页

Fungal – assisted microalgae flocculation and simultaneous lignocellulolytic enzyme production in wastewater treatment systems 真菌辅助微藻絮凝和废水处理系统中木质素纤维素水解酶的同步生产

Biotechnology Reports Pub Date : 2025-01-10 DOI: 10.1016/j.btre.2025.e00875

Anna Civzele, Linda Mezule

{"title":"Fungal – assisted microalgae flocculation and simultaneous lignocellulolytic enzyme production in wastewater treatment systems","authors":"Anna Civzele, Linda Mezule","doi":"10.1016/j.btre.2025.e00875","DOIUrl":"10.1016/j.btre.2025.e00875","url":null,"abstract":"<div><div>The study investigates the application of white rot fungi for reactor–scale microalgae harvesting and explores the mechanisms underlying the algal–fungal interactions and their impact on biomass composition. Enzymatic analysis and microscopy revealed that the formation of algal-fungal complexes and successful harvesting are coupled with fungal cellulose-degrading enzyme production and hydrolytic processes of microalgae cells. Fluorescence intensity decreased by over 80 % in cells stained with Calcofluor-white after interaction with white rot fungi, indicating the reduction in cellulose content in microalgal cells caused by fungal enzymatic activity. These enzymes also caused significant cell damage and more than 50 % decrease in microalgae cell size. The presence of cellulolytic enzymes broadens the potential application of the resulting biomass in various biotechnological applications. Moreover, reactor-scale bioflocculation resulted in over 95 % <em>T. obliquus</em> and almost 85 % <em>C. vulgaris</em> harvesting efficiency from secondary wastewater within less than 24 h, demonstrating the method's scalability and industrial applicability.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00875"},"PeriodicalIF":0.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cobalt phosphide-loaded biochar synthesis using phosphate-accumulating yeast and its application as an electrocatalyst 聚磷酵母合成负载磷酸钴的生物炭及其电催化剂的应用。

Biotechnology Reports Pub Date : 2025-01-09 DOI: 10.1016/j.btre.2025.e00874

Yoshihiro Ojima , Riho Akiyoshi , Itto Tokiwa , Takashi Nakazono , Yusuke Yamada , Masayuki Azuma

{"title":"Cobalt phosphide-loaded biochar synthesis using phosphate-accumulating yeast and its application as an electrocatalyst","authors":"Yoshihiro Ojima , Riho Akiyoshi , Itto Tokiwa , Takashi Nakazono , Yusuke Yamada , Masayuki Azuma","doi":"10.1016/j.btre.2025.e00874","DOIUrl":"10.1016/j.btre.2025.e00874","url":null,"abstract":"<div><div>A phosphorus (P)-accumulating mutant strain of <em>Saccharomyces cerevisiae</em> is used as a precursor for transition metal phosphides (TMPs) biochar. Tetrahydrofuran treatment of the P-accumulating yeast coupled with pyrolysis resulted in the formation of CoP-loaded biochar (CoP@P-yeast) unlike previously reported Co<sub>2</sub>P-loaded biochar using dry baker's yeast. The CoP@P-yeast exhibited the electrocatalytic activity for the hydrogen evolution with an overpotential of −192 mV at 10 mA cm<sup>−2</sup>. Furthermore, the CoP@P-yeast showed the highest ammonia production rate of 33 mg-NH<sub>3</sub> h<sup>−1</sup> mg-catalyst<sup>−1</sup> in nitrate reduction reaction, as well as much higher than that with platinum on graphitized carbon. Scanning electron microscopy and transmission electron microscopy observations revealed that relatively large TMP crystals mainly located at the biochar surface, which may be beneficial to avoid catalytic deterioration during the nitrate reduction reaction. This study demonstrates that P-accumulating mutant strain of yeast is a suitable precursor to improve the activity of the resulting TMP biochar.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00874"},"PeriodicalIF":0.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of agro-industrial waste through solid-state fermentation: Mini review 农业工业废弃物的固态发酵增值研究进展

Biotechnology Reports Pub Date : 2024-12-30 DOI: 10.1016/j.btre.2024.e00873

Mohammad Perwez , Sameer Al Asheh

{"title":"Valorization of agro-industrial waste through solid-state fermentation: Mini review","authors":"Mohammad Perwez , Sameer Al Asheh","doi":"10.1016/j.btre.2024.e00873","DOIUrl":"10.1016/j.btre.2024.e00873","url":null,"abstract":"<div><div>Agriculture and industrial waste are produced in large volumes every year worldwide, causing serious concerns about their disposal. These wastes have high organic content, which microorganisms can easily assimilate into relevant value-added products. Valorization of agro-industrial waste is required for sustainable development. Solid state fermentation is an excellent method of utilizing waste for circular bioeconomy. Exploitation of agro-industrial waste as a substrate utilizing microorganisms for solid state fermentation provides beneficial products for use in industries and other fields. The use of waste reduces the cost of production of value-added products. This method is an environmentally friendly, economical and feasible approach for waste management. This review discusses the factors affecting the production of value-added products through solid state fermentation. It also discusses the valuable products from solid state fermentation technology, such as antibiotics, enzymes, organic acids, bioremediation, biosurfactants and biofertilizers. Challenges and future prospects are also presented.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00873"},"PeriodicalIF":0.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriophage-based biosensors technology: Materials, fabrications, efficiencies and shortcomings 基于噬菌体的生物传感器技术：材料、制造、效率和缺陷。

Biotechnology Reports Pub Date : 2024-12-21 DOI: 10.1016/j.btre.2024.e00872

Ihtisham Ul Haq , Kashif Rahim , Sajida Maryam , Najeeba Parre Paker

{"title":"Bacteriophage-based biosensors technology: Materials, fabrications, efficiencies and shortcomings","authors":"Ihtisham Ul Haq , Kashif Rahim , Sajida Maryam , Najeeba Parre Paker","doi":"10.1016/j.btre.2024.e00872","DOIUrl":"10.1016/j.btre.2024.e00872","url":null,"abstract":"<div><div>Ongoing research in biosensor technologies has led to advanced functional materials for healthcare diagnostics, and bacteriophages (phages), demonstrating exceptional utility due to their high specificity, accuracy, rapid, label-free, and wireless detection capabilities with minimal false-positive results. Phage-based-pathogen-detecting biosensors (PBPDBs) include surface plasmon resonance (SPR) biosensors, magnetoelastic (ME), electrochemical, and quartz crystal microbalance (QCM) biosensors. Commonly used substrates for PBPDBs are gold, silicon, glass, carbon-based materials, magnetic particles, and quantum dots. These substrates are chemically and physically modified to optimize phage orientation on sensor surfaces, enhancing bacterial capture. To address typical stability and issues encountered in traditional biosensor applications, phage particles and genetically modified phages are utilized to improve biosensor stability and increase detection efficacy while reducing assay time. Genetic modification in phages facilitated by CRISPR/Cas9 enables the tailoring of phages to target specific bacterial strains. This approach helps overcome the inherent specificity of phages and enables the detection of multiple pathogens in a single assay. Multiple pathogens can be detected through a single phage-based assay. This manuscript elucidates the fabrication methodologies and detection efficiencies of PBPDBs providing valuable insights into the development of practical, precise, and efficient biosensors for pathogen detection.</div></div><div><h3>Summary</h3><div>PBPDBs are emerging diagnostic tools for the detection of bacterial pathogens.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00872"},"PeriodicalIF":0.0,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective role of savory essential oil on vital organs in rats against deleterious effects induced by lead acetate 香薰精油对大鼠重要器官抗醋酸铅毒性作用的保护作用。

Biotechnology Reports Pub Date : 2024-12-16 DOI: 10.1016/j.btre.2024.e00871

Doha H. Aboubaker , Nermeen A. Shaffie , Mona F. Shabana , Ahmed Abd Elghafour , Bassant M.M. Ibrahim

{"title":"Protective role of savory essential oil on vital organs in rats against deleterious effects induced by lead acetate","authors":"Doha H. Aboubaker , Nermeen A. Shaffie , Mona F. Shabana , Ahmed Abd Elghafour , Bassant M.M. Ibrahim","doi":"10.1016/j.btre.2024.e00871","DOIUrl":"10.1016/j.btre.2024.e00871","url":null,"abstract":"<div><div>The damaging effects of heavy metal exposure on vital organs like the heart, liver, kidneys, and brain can be lowered by natural compounds' anti-inflammatory and anti-oxidant capacity. In the current investigation, the protective potential of savory(<em>Saturejahortensis</em>)essential oil (EO) against lead acetate-induced multi-organ damage in rats was evaluated. Thirty female Wister Albino rats were divided into the following groups: normal, positive control given lead acetate without concomitant treatment, reference given ethylene-diamine-tetra-acetic acid and groups treated with savory EO (0.5 and 1 ml/rat), treatments were administered concomitantly with lead acetate for ten successive days. Electrocardiogram (ECG), open field, and rota-rod tests for evaluation of behaviour, as well as TNF-α, R-GSH & MDA were measured in brain and liver homogenates respectively, CRP, liver, kidney, and heart function tests, were measured in serum. Histopathology of vital organs was conducted at the end of the experiment.A high dose of savory EO, significantly improved the cardiac electrophysiology, psychological state, and locomotor activity of rats; moreover, it lowered inflammatory, oxidative biomarkers, liver, kidney, and cardiac function tests of the rats. Savory EO protects vital organs against heavy metal damage with the superiority of the high dose.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00871"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142985051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative strategies for utilizing microalgae as dual-purpose biofertilizers and phycoremediators in agroecosystems 利用微藻作为农业生态系统中双重用途生物肥料和藻修复剂的创新策略。

Biotechnology Reports Pub Date : 2024-12-10 DOI: 10.1016/j.btre.2024.e00870

Muhamad Maulana Azimatun Nur , Mahreni , Sri Wahyu Murni , Tutik Muji Setyoningrum , Faizah Hadi , Tunjung Wahyu Widayati , Danang Jaya , Raden Roro Endang Sulistyawati , Dwi Aulia Puspitaningrum , Resti Nurmala Dewi , Hadiyanto , M. Hasanuzzaman

{"title":"Innovative strategies for utilizing microalgae as dual-purpose biofertilizers and phycoremediators in agroecosystems","authors":"Muhamad Maulana Azimatun Nur , Mahreni , Sri Wahyu Murni , Tutik Muji Setyoningrum , Faizah Hadi , Tunjung Wahyu Widayati , Danang Jaya , Raden Roro Endang Sulistyawati , Dwi Aulia Puspitaningrum , Resti Nurmala Dewi , Hadiyanto , M. Hasanuzzaman","doi":"10.1016/j.btre.2024.e00870","DOIUrl":"10.1016/j.btre.2024.e00870","url":null,"abstract":"<div><div>The increasing need for sustainable agricultural practices due to the overuse of chemical fertilizers has prompted interest in microalgae as biofertilizers. This review investigates the potential of microalgae as biofertilizers and phycoremediators within sustainable agroecosystems, addressing both soil fertility and wastewater management. Microalgae provide a dual benefit by absorbing excess nutrients and contaminants from wastewater, generating nutrient-rich biomass that can replace chemical fertilizers and support plant growth. Implementation strategies include cultivating microalgae in wastewater to offset production costs, using closed photobioreactor systems to enhance growth efficiency, and applying microalgal biomass directly to soil or crops. Additionally, microalgae extracts provide essential bioactive compounds, such as phytohormones and amino acids, that enhance plant growth and resilience. While microalgae offer an eco-friendly solution for nutrient recycling and crop productivity, challenges in scalability, production cost, and regulatory frameworks hinder widespread adoption. This review highlights the potential pathways and technological advancements necessary for integrating microalgae into sustainable agriculture, emphasizing the need for interdisciplinary collaboration and innovative approaches to overcome these barriers. Ultimately, microalgae biofertilizers represent a promising approach to reducing environmental impact and advancing sustainable farming practices.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00870"},"PeriodicalIF":0.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11700267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prospective identification of extracellular triacylglycerol hydrolase with conserved amino acids in Amycolatopsis tolypomycina’s high G+C genomic dataset 在粘菌Amycolatopsis tolypomycina的高G+C基因组数据集中具有保守氨基酸的胞外三酰基甘油水解酶的前瞻性鉴定。

Biotechnology Reports Pub Date : 2024-12-07 DOI: 10.1016/j.btre.2024.e00869

Supajit Sraphet , Bagher Javadi

{"title":"Prospective identification of extracellular triacylglycerol hydrolase with conserved amino acids in Amycolatopsis tolypomycina’s high G+C genomic dataset","authors":"Supajit Sraphet , Bagher Javadi","doi":"10.1016/j.btre.2024.e00869","DOIUrl":"10.1016/j.btre.2024.e00869","url":null,"abstract":"<div><div>Extracellular triacylglycerol hydrolases (ETH) play a critical role for microorganisms, acting as essential tools for lipid breakdown and survival in challenging environments. The pursuit of more effective ETH genes and enzymes through evolution holds significant potential for enhancing living conditions. This study employs a proteogenomic approach to identify high G+C ETH in a notable Gram-positive bacterium, <em>Amycolatopsis tolypomycina</em>. Utilizing knowledge from genome and machine learning algorithms, prospective ETH genes/enzymes were identified. Notably, the ETH structural conserved accessibility to solvent clearly indicated the specific sixteen residues (GLY50, PRO93, GLY141, ASP148, GLY151, ASP172, ALA176, GLY195, TYR196, SER197, GLN198, GLY199, GLY200, GLY225, PRO327, ASP336) with no frequency. By pinpointing key residues and understanding their role, this study sets the stage for enhancing ETH performance through computational proteogenomic and contributes to the broader field of enzyme engineering, facilitating the development of more efficient and versatile ETH enzymes tailored to specific industrial or environmental contexts.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00869"},"PeriodicalIF":0.0,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light intensity effects on bioproduct recovery from fuel synthesis wastewater using purple phototrophic bacteria in a hybrid biofilm-suspended growth system 光强对混合生物膜悬浮生长系统中紫色光养细菌从燃料合成废水中回收生物产品的影响

Biotechnology Reports Pub Date : 2024-12-01 DOI: 10.1016/j.btre.2024.e00863

Sultan Shaikh , Gordon McKay , Hamish Robert Mackey

{"title":"Light intensity effects on bioproduct recovery from fuel synthesis wastewater using purple phototrophic bacteria in a hybrid biofilm-suspended growth system","authors":"Sultan Shaikh , Gordon McKay , Hamish Robert Mackey","doi":"10.1016/j.btre.2024.e00863","DOIUrl":"10.1016/j.btre.2024.e00863","url":null,"abstract":"<div><div>This research looked at how three different light intensities (1600, 4300, and 7200 lx) affect the biomass development, treatment of fuel synthesis wastewater and the recovery of valuable bioproducts between biofilm and suspended growth in a purple-bacteria enriched photobioreactor. Each condition was run in duplicate using an agricultural shade cloth as the biofilm support media in a continuously mixed batch reactor. The results showed that the highest chemical oxygen demand (COD) removal rate (56.8 ± 0.9 %) was found under the highest light intensity (7200 lx), which also led to the most biofilm formation and highest biofilm biomass production (1225 ± 95.7 mg). The maximum carotenoids (Crts) and bacteriochlorophylls (BChls) content occurred in the suspended growth of the 7200 lx reactor. BChls decreased with light intensity in suspended growth, while in biofilm both Crts and BChls were relatively stable between light conditions, likely due to an averaging effect as biofilm thickened at higher light intensity. Light intensity did not affect protein content of the biomass, however, biofilm showed a lower average (41.2 % to 43.7 %) than suspended biomass (45.4 % to 47.7 %). For polyhydroxybutyrate (PHB) the highest cell concentration in biofilm occurred at 1600 lx (11.4 ± 2.4 %), while for suspended growth it occurred at 7200 lx (22.7 ± 0.3 %), though total PHB productivity remained similar between reactors. Shading effects from the externally located biofilm could explain most variations in bioproduct distribution. Overall, these findings suggest that controlling light intensity can effectively influence the treatment of fuel synthesis wastewater and the recovery of valuable bioproducts in a biofilm photobioreactor.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"44 ","pages":"Article e00863"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of microbial antagonistic activity and Quorum Sensing Signal Molecule (Cyclopeptides-DKPs and N-Acyl Homoserine Lactones) detection in bacterial strains obtained from avocado thrips (Thysanoptera: Thripidae) 牛油果蓟马微生物拮抗活性评价及群体感应信号分子（环肽- dkps和n -酰基同型丝氨酸内酯）检测

Biotechnology Reports Pub Date : 2024-11-29 DOI: 10.1016/j.btre.2024.e00866

A.N. Pereira-Bazurdo , G.E. Cadavid-Restrepo , R.E. Arango-Isaza , C.X. Moreno-Herrera

{"title":"Assessment of microbial antagonistic activity and Quorum Sensing Signal Molecule (Cyclopeptides-DKPs and N-Acyl Homoserine Lactones) detection in bacterial strains obtained from avocado thrips (Thysanoptera: Thripidae)","authors":"A.N. Pereira-Bazurdo , G.E. Cadavid-Restrepo , R.E. Arango-Isaza , C.X. Moreno-Herrera","doi":"10.1016/j.btre.2024.e00866","DOIUrl":"10.1016/j.btre.2024.e00866","url":null,"abstract":"<div><div>The control of avocado pests and diseases heavily relies on the use of several types of pesticides, some of which are strictly monitored or not internationally accepted. New sources of bioactive molecules produced by phytopathogen-inhibiting microorganisms offer an excellent alternative for the control of pests and diseases. This study explores the potential antagonistic action against phytopathogenic microorganisms, using bacterial strains obtained from avocado thrips. In addition, we detected and identified quorum sensing (QS) signaling molecules that are related to virulence factors and antibiotic production. The results showed that <em>Bacillus, Pantoea</em>, and <em>Serratia</em> strains exhibited antagonism against five fungal phytopathogens. Additionally, some bacteria also produce specific signaling molecules like N-3-(oxododecanoyl)-l-homoserine lactone (OdDHL), N-(3-oxo)-hexanoyl l-HL (OHHL), 4‑hydroxy-2-heptylquinoline (HHQ) or 2-heptyl-3,4-dihydroxyquinoline (PQS, Pseudomonas quinolone signal), cyclo(L-Phe-l-Pro), and cyclo(L-Pro-l-Tyr, which might give them antimicrobial properties. This research explores the biotechnological potential of these bacteria in fighting the diseases affecting avocados in Colombia.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00866"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of Natterins-based peptides improves antimicrobial and antiviral activities 基于纳豆素的多肽的设计提高了抗菌和抗病毒活性。

Biotechnology Reports Pub Date : 2024-11-28 DOI: 10.1016/j.btre.2024.e00867

Gabrielle L. de Cena , Dayane B. Tada , Danilo B.M. Lucchi , Tiago A.A. Santos , Montserrat Heras , Maria Juliano , Carla Torres Braconi , Miguel A.R.B. Castanho , Mônica Lopes-Ferreira , Katia Conceição

{"title":"Design of Natterins-based peptides improves antimicrobial and antiviral activities","authors":"Gabrielle L. de Cena , Dayane B. Tada , Danilo B.M. Lucchi , Tiago A.A. Santos , Montserrat Heras , Maria Juliano , Carla Torres Braconi , Miguel A.R.B. Castanho , Mônica Lopes-Ferreira , Katia Conceição","doi":"10.1016/j.btre.2024.e00867","DOIUrl":"10.1016/j.btre.2024.e00867","url":null,"abstract":"<div><div>The biochemical analysis of animal venoms has been intensifying over the years, enabling the prediction of new molecules derived from toxins, harnessing the therapeutic potential of these molecules. From the venom of the fish <em>Thalassophryne nattereri</em>, using <em>in silico</em> methods for predicting antimicrobial and cell-penetrating peptides, two peptides from Natterins with promising characteristics were synthesized and subjected to <em>in vitro</em> and <em>in vivo</em> analysis. The peptides were subjected to stability tests and antimicrobial assays, cytotoxicity in murine fibroblast cells, antiviral assays against the Chikungunya virus, and the toxicity on <em>G. mellonella</em> was also evaluated. The findings underscore the peptides' robust stability under varying temperatures and pH conditions and resistance to proteolytic degradation. The peptides demonstrated significant antimicrobial efficacy, minimal cytotoxicity, and low hemolytic activity. Although their antiviral efficacy was limited, they showed potential at specific stages of viral replication. The <em>in vivo</em> toxicity tests indicated a favorable safety profile. These findings suggest that this approach can aid in the development of antimicrobial agents, offering a faster and personalized method to combat microbial infections, and represent a promising discovery in venom biotechnology research.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00867"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0