盐度诱导盐敏感型和耐盐型甘蔗酯酶、过氧化物酶和乙醇脱氢酶同工酶及叶片可溶性蛋白的变化

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2025-02-05 DOI:10.1016/j.btre.2025.e00880

Manisha Rameshrao Patil , A.A. Kale , Ajay Kumar Singh , Priyanka Rameshrao Patil , Shaheen Badshah Inamdar , R.D. Satbhai

{"title":"盐度诱导盐敏感型和耐盐型甘蔗酯酶、过氧化物酶和乙醇脱氢酶同工酶及叶片可溶性蛋白的变化","authors":"Manisha Rameshrao Patil , A.A. Kale , Ajay Kumar Singh , Priyanka Rameshrao Patil , Shaheen Badshah Inamdar , R.D. Satbhai","doi":"10.1016/j.btre.2025.e00880","DOIUrl":null,"url":null,"abstract":"<div><div>The salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 were evaluated for Peroxidase (POX), Esterase (EST) and Alcohol Dehydrogenase (ADH) isozymes and soluble protein profiling by SDS and native-PAGE at salinity levels 0.41 dSm<sup>-1</sup>, 2.31 dSm<sup>-1</sup>, 4.21 dSm<sup>-1</sup>, and 8.01 dSm<sup>-1</sup> maintained by NaCl solution. The plant height, number of leaves and seedling diameter got reduced in salinity susceptible sugarcane genotype CoC-671 as well as salinity tolerant sugarcane genotype CoM-265 with increase in salinity levels. However, reduction in plant height, number of leaves and seedling diameter was less in salinity tolerant sugarcane genotype CoM-265 as compared to salinity susceptible sugarcane genotype CoC-671. The POX isozyme profiling revealed that salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 had variation in soluble protein band intensity at different salinity levels with relative mobility (Rm) 0.137. The present study could be useful for genetic variability analysis in sugarcane genotypes differing in salinity stress tolerance capability.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"45 ","pages":"Article e00880"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Salinity induced changes in esterase, peroxidase and alcohol dehydrogenase isozymes and leaf soluble proteins in salinity susceptible and salinity tolerant sugarcane genotypes\",\"authors\":\"Manisha Rameshrao Patil , A.A. Kale , Ajay Kumar Singh , Priyanka Rameshrao Patil , Shaheen Badshah Inamdar , R.D. Satbhai\",\"doi\":\"10.1016/j.btre.2025.e00880\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 were evaluated for Peroxidase (POX), Esterase (EST) and Alcohol Dehydrogenase (ADH) isozymes and soluble protein profiling by SDS and native-PAGE at salinity levels 0.41 dSm<sup>-1</sup>, 2.31 dSm<sup>-1</sup>, 4.21 dSm<sup>-1</sup>, and 8.01 dSm<sup>-1</sup> maintained by NaCl solution. The plant height, number of leaves and seedling diameter got reduced in salinity susceptible sugarcane genotype CoC-671 as well as salinity tolerant sugarcane genotype CoM-265 with increase in salinity levels. However, reduction in plant height, number of leaves and seedling diameter was less in salinity tolerant sugarcane genotype CoM-265 as compared to salinity susceptible sugarcane genotype CoC-671. The POX isozyme profiling revealed that salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 had variation in soluble protein band intensity at different salinity levels with relative mobility (Rm) 0.137. The present study could be useful for genetic variability analysis in sugarcane genotypes differing in salinity stress tolerance capability.</div></div>\",\"PeriodicalId\":38117,\"journal\":{\"name\":\"Biotechnology Reports\",\"volume\":\"45 \",\"pages\":\"Article e00880\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-02-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2215017X25000074\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Immunology and Microbiology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2215017X25000074","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Immunology and Microbiology","Score":null,"Total":0}

引用次数: 0

摘要

以盐敏感型CoC-671和耐盐甘蔗基因型CoM-265为材料，在盐浓度为0.41、2.31、4.21和8.01的NaCl溶液中，用SDS和原生page分析了过氧化物酶（POX）、酯酶（EST）和醇脱氢酶（ADH）同工酶和可溶性蛋白谱。耐盐基因型CoC-671和耐盐基因型CoM-265的株高、叶片数和幼苗直径均随盐度水平的升高而降低。耐盐基因型CoM-265的株高、叶片数和幼苗直径的降低幅度小于耐盐基因型CoC-671。同工酶谱分析显示，耐盐基因型CoC-671和耐盐基因型CoM-265在不同盐度水平下可溶性蛋白带强度存在差异，相对迁移率（Rm）为0.137。本研究可为甘蔗耐盐能力不同基因型的遗传变异分析提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Salinity induced changes in esterase, peroxidase and alcohol dehydrogenase isozymes and leaf soluble proteins in salinity susceptible and salinity tolerant sugarcane genotypes

The salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 were evaluated for Peroxidase (POX), Esterase (EST) and Alcohol Dehydrogenase (ADH) isozymes and soluble protein profiling by SDS and native-PAGE at salinity levels 0.41 dSm^-1, 2.31 dSm^-1, 4.21 dSm^-1, and 8.01 dSm^-1 maintained by NaCl solution. The plant height, number of leaves and seedling diameter got reduced in salinity susceptible sugarcane genotype CoC-671 as well as salinity tolerant sugarcane genotype CoM-265 with increase in salinity levels. However, reduction in plant height, number of leaves and seedling diameter was less in salinity tolerant sugarcane genotype CoM-265 as compared to salinity susceptible sugarcane genotype CoC-671. The POX isozyme profiling revealed that salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 had variation in soluble protein band intensity at different salinity levels with relative mobility (Rm) 0.137. The present study could be useful for genetic variability analysis in sugarcane genotypes differing in salinity stress tolerance capability.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.