Improvement of protein quality and reduction of anti-nutritional factors in soybean meal by solid-state fermentation with Bacillus siamensis MH03

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2025-08-13 DOI:10.1016/j.btre.2025.e00915

Tanawut Chotinu-kul, Gunjana Theeragool, Duenrut Chonudomkul

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Abstract

This study investigated the optimal conditions for enhancing protein quality and reducing anti-nutritional factors in soybean meal (SBM) through solid-state fermentation (SSF) with Bacillus. Among 78 isolates, strain MH03 exhibited the highest extracellular enzyme production and efficacy in enhancing SBM quality. Under optimized SSF conditions of 5 kg SBM, 5 cm thickness and 50 % initial moisture for 72 h, strain MH03 increased soluble protein from 3.31±0.21 to 10.16±0.27 g/100 g and significantly reduced anti-nutritional compounds, including glycinin, β-conglycinin, trypsin inhibitor, and oligosaccharides. Crude protein content increased from 47.89±0.07 % to 55.84±0.25 %, with protein solubility increased from 74.96±0.45 % to 90.54±0.28 %, with statistically significant increases. Other nutritional values were also significantly enhanced. Essential amino acids, such as valine, methionine, isoleucine, and lysine also increased. Based on 16S rRNA gene sequencing, MH03 was identified as Bacillus siamensis. These findings highlight the potential of B. siamensis MH03-fermented SBM as a high-quality protein ingredient for animal feed applications.

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暹芽孢杆菌MH03固态发酵提高豆粕蛋白质品质和降低抗营养因子

以豆粕为原料，研究了芽孢杆菌固态发酵提高豆粕蛋白质品质、降低抗营养因子的最佳条件。在78株分离菌株中，菌株MH03的胞外酶产量最高，提高SBM品质的效果最好。在优化的SSF条件下（5 kg SBM， 5 cm厚度，50%初始湿度），菌株MH03可将可溶性蛋白从3.31±0.21 g/100 g提高到10.16±0.27 g/100 g，并显著降低抗营养化合物，包括甘氨酸、β-甘氨酸、胰蛋白酶抑制剂和低聚糖。粗蛋白质含量由47.89±0.07%提高到55.84±0.25%，蛋白质溶解度由74.96±0.45%提高到90.54±0.28%，差异均有统计学意义。其他营养价值也显著提高。必需氨基酸，如缬氨酸、蛋氨酸、异亮氨酸和赖氨酸也增加了。通过16S rRNA基因测序，鉴定MH03为暹罗芽孢杆菌。这些发现突出了暹罗芽孢杆菌mh03发酵SBM作为动物饲料中高质量蛋白质成分的潜力。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.