Biodecolorization and Biodegradation of Methyl Orange by Immobilized Pseudomonas aeruginosa Bacterium into SA/PVA Matrix Integrated with MOF UiO-66 Adsorbent

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2025-09-05 DOI:10.1016/j.btre.2025.e00924

Sarazen Shalahuddin Akbar, Adi Setyo Purnomo , Silvia Abdi Pratama, Berlian Imazdalifa, Sifra Thrivelia Krisnia Ayu, Reyza Fadly Maghfiroh, Nur Annisa Kusumawardhani, Ardi Lukman Hakim

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引用次数: 0

Abstract

Azo dye, such as methyl orange (MO), is characterized by environmental concerns due to persistence, toxicity, and potential carcinogenic effects. Therefore, this study aimed to investigate the integration of immobilized Pseudomonas aeruginosa (PA) and UiO-66, a zirconium-based metal-organic framework (MOF), into sodium alginate/polyvinyl alcohol (SA/PVA) matrix for biodecolorization and biodegradation of MO dye. Material characterization was carried out by using X-ray diffraction (XRD), Fourier-transform infrared (FTIR), and scanning electron microscopy-energy dispersive X-ray (SEM-EDX) which showed the successful formation of UiO-66 and stable incorporation in SA/PVA/PA beads. The results of adsorption experiments showed that the presence of UiO-66 enhanced MO adsorption capacity by 20.47 % compared to SA/PVA/PA matrix without UiO-66. Kinetic analysis followed the pseudo-second-order model, and adsorption isotherm was best described by the Langmuir model, showing monolayer adsorption behavior. Furthermore, biodecolorization results showed that SA/PVA/UiO-66/PA beads achieved a 92.03 % MO removal rate, indicating a 33.99 % higher than free PA cells. Liquid chromatography–mass spectrometry (LC-MS) analysis confirmed the formation of nine biodegradation products, suggesting successful structural breakdown of MO. These results showed that combining UiO-66 with immobilized PA significantly enhanced both adsorption and biodegradation performance toward MO treatment.

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固定化铜绿假单胞菌对SA/PVA复合MOF UiO-66吸附剂对甲基橙的生物脱色降解

偶氮染料，如甲基橙（MO），因其持久性、毒性和潜在的致癌作用而受到环境问题的关注。因此，本研究旨在研究将固定化铜绿假单胞菌（PA）和锆基金属有机骨架（MOF） UiO-66整合到海藻酸钠/聚乙烯醇（SA/PVA）基质中，用于MO染料的生物脱色和生物降解。通过x射线衍射（XRD）、傅里叶变换红外（FTIR）和扫描电镜-能量色散x射线（SEM-EDX）对材料进行了表征，结果表明UiO-66成功形成，并稳定地结合在SA/PVA/PA微珠中。吸附实验结果表明，与不含UiO-66的SA/PVA/PA基质相比，UiO-66的存在使MO吸附量提高了20.47%。动力学分析采用拟二阶模型，Langmuir模型最能描述吸附等温线，呈现单层吸附行为。此外，生物脱色结果表明，SA/PVA/UiO-66/PA微球对MO的去除率达到92.03%，比游离PA细胞高出33.99%。液相色谱-质谱（LC-MS）分析证实了9种生物降解产物的形成，表明MO的结构被成功分解。这些结果表明，UiO-66与固定化PA的结合显著提高了对MO的吸附和生物降解性能。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.