Biotechnology Reports最新文献_第9页

Vegetative insecticidal protein (Vip3A) production by Bacillus thuringiensis Bt294 and its efficacy against Lepidopteran pests (Spodoptera exigua) 苏云金芽孢杆菌Bt294生产的植物性杀虫蛋白Vip3A及其对鳞翅目害虫的防治效果

Biotechnology Reports Pub Date : 2023-09-12 DOI: 10.1016/j.btre.2023.e00812

Pumin Nutaratat , Borworn Werapan , Netnapa Phosrithong , Chutchanun Trakulnaleamsai , Amporn Rungrod , Mongkon Utamatho , Sumarin Soonsanga , Boonhiang Promdonkoy , Kwanruthai Malairuang , Wai Prathumpai

{"title":"Vegetative insecticidal protein (Vip3A) production by Bacillus thuringiensis Bt294 and its efficacy against Lepidopteran pests (Spodoptera exigua)","authors":"Pumin Nutaratat , Borworn Werapan , Netnapa Phosrithong , Chutchanun Trakulnaleamsai , Amporn Rungrod , Mongkon Utamatho , Sumarin Soonsanga , Boonhiang Promdonkoy , Kwanruthai Malairuang , Wai Prathumpai","doi":"10.1016/j.btre.2023.e00812","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00812","url":null,"abstract":"<div>A vegetative insecticidal protein, Vip3A, is highly active against lepidopteran pests, which are the most important pests in most tropical countries. An important aspect of the successful commercial production of this bacterial insecticide is the development of bacterial culture media that maximize the titres of this protein and cost reduction. This study aimed to investigate and optimize Vip3A production by Bacillus thuringiensis Bt294 using statistical methods and 3-step sequential approaches. The experimental design showed that the production of Vip3A was maximized to 300 mg/L when the bacterium was cultivated in medium composed of 5.05 g/L glycerol, 49.17 g/L soytone, 30.05 g/L casein hydrolysate, 1.99 g/L CaCl2.2H2O, 7.5 mg/L CuSO4, 15 mg/L MnSO4.H2O, 9.4 g/L K2HPO4, 2.2 g/L KH2PO4, 0.2 g/L MgSO4.7H2O, 5 g/L yeast extract, 2.5 mg/L NiCl2.6H2O and 3 mL/L vitamin solution. B. thuringiensis Bt294 Vip3A toxin was highly toxic to Spodoptera exigua with LC50 values of 187.1 ng/cm2 at 7 days. This result demonstrated that a high titre of Vip3A produced by B. thuringiensis Bt294 will be useful as a biological control agent. This optimization will allow production to be scaled up for commercial production in the future.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00812"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50173530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibition of bacterial biofilms by the snake venom proteome 蛇毒蛋白质组对细菌生物膜的抑制作用

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00810

Neyaz A. Khan , Fernanda G. Amorim , John P. Dunbar , Dayle Leonard , Damien Redureau , Loïc Quinton , Michel M. Dugon , Aoife Boyd

{"title":"Inhibition of bacterial biofilms by the snake venom proteome","authors":"Neyaz A. Khan , Fernanda G. Amorim , John P. Dunbar , Dayle Leonard , Damien Redureau , Loïc Quinton , Michel M. Dugon , Aoife Boyd","doi":"10.1016/j.btre.2023.e00810","DOIUrl":"10.1016/j.btre.2023.e00810","url":null,"abstract":"<div>Snake venoms possess a range of pharmacological and toxicological activities. Here we evaluated the antibacterial and anti-biofilm activity against methicillin-susceptible and methicillin-resistant Staphylococcus aureus (MSSA and MRSA) of venoms from the Samar spitting cobra Naja samarensis and the Puff adder Bitis arietans. Both venoms prevented biofilm production by pathogenic S. aureus in a growth-independent manner, with the B. arietans venom being most potent. Fractionation showed the active molecule to be heat-labile and >10 kDa in size. Proteomic profiles of N. samarensis venom revealed neurotoxins and cytotoxins, as well as an abundance of serine proteases and three-finger toxins, while serine proteases, metalloproteinases and C-lectin types were abundant in B. arietans venom. These enzymes may have evolved to prevent bacteria colonising the snake venom gland. From a biomedical biotechnology perspective, they have valuable potential for anti-virulence therapy to fight antibiotic resistant microbes.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00810"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10407894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9973050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A high throughput screening process and quick isolation of novel lignin-degrading microbes from large number of natural biomasses 一种高通量筛选方法及从大量天然生物质中快速分离新型木质素降解微生物

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00809

Nadia Sufdar Ali , Fang Huang , Wensheng Qin , Trent Chunzhong Yang PhD

{"title":"A high throughput screening process and quick isolation of novel lignin-degrading microbes from large number of natural biomasses","authors":"Nadia Sufdar Ali , Fang Huang , Wensheng Qin , Trent Chunzhong Yang PhD","doi":"10.1016/j.btre.2023.e00809","DOIUrl":"10.1016/j.btre.2023.e00809","url":null,"abstract":"<div>High throughput screening approaches can significantly speed up the identification of novel enzymes from natural microbial consortiums. A two-step high throughput screening process was proposed and explored to screen lignin-degrading microorganisms. By employing this modified culture enrichment method and screening based on enzyme activity, a total of 82 bacterial and 46 fungal strains were isolated from fifty decayed wood samples (100 liquid cultures) collected from the banks of the Ottawa River in Canada. Among them, ten bacterial and five fungal strains were selected and identified based on their high laccase activities by 16S rDNA and ITS gene sequencing, respectively. The study identified bacterial strains from various genera including Serratia, Enterobacter, Raoultella, and Bacillus, along with fungal counterparts including Mucor, Trametes, Conifera and Aspergillus. Moreover, Aspergillus sydowii (AORF21), Mucor sp. (AORF43), Trametes versicolor (AORF3) and Enterobacter sp. (AORB55) exhibited xylanase and β- glucanase activities in addition to laccase production. The proposed approach allowed for the quick identification of promising consortia and enhanced the chance of isolating desired strains based on desired enzyme activities. This method is not limited to lignocellulose and lignin-degrading microorganisms but can be applied to identify novel microbial strains and enzymes from different natural samples.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00809"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3e/c3/main.PMC10423689.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10012649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, and screening of biosurfactant-producing and hydrocarbon-degrading bacteria from oil and gas industrial waste 石油天然气工业废弃物中产生生物表面活性剂和降解碳氢化合物细菌的分离、鉴定和筛选

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00804

S. Al-Marri , H.I. Eldos , M.Y. Ashfaq , S. Saeed , S. Skariah , L. Varghese , Y.A. Mohamoud , A.A. Sultan , M.M. Raja

{"title":"Isolation, identification, and screening of biosurfactant-producing and hydrocarbon-degrading bacteria from oil and gas industrial waste","authors":"S. Al-Marri , H.I. Eldos , M.Y. Ashfaq , S. Saeed , S. Skariah , L. Varghese , Y.A. Mohamoud , A.A. Sultan , M.M. Raja","doi":"10.1016/j.btre.2023.e00804","DOIUrl":"10.1016/j.btre.2023.e00804","url":null,"abstract":"<div>Qatar is one of the biggest oil and gas producers in the world, coupled with it is challenging environmental conditions (high average temperature: >40 °C, low annual rainfall: 46.71 mm, and high annual evaporation rate: 2200 mm) harbors diverse microbial communities that are novel and robust, with the potential to biodegrade hydrocarbons. In this study, we collected hydrocarbon contaminated sludge, wastewater and soil samples from oil and gas industries in Qatar. Twenty-six bacterial strains were isolated in the laboratory from these samples using high saline conditions and crude oil as the sole carbon source. A total of 15 different bacterial genera were identified in our study that have not been widely reported in the literature or studied for their usage in the biodegradation of hydrocarbons. Interestingly, some of the bacteria that were identified belonged to the same genus however, demonstrated variable growth rates and biosurfactant production. This indicates the possibility of niche specialization and specific evolution to acquire competitive traits for better survival. The most potent strain EXS14, identified as Marinobacter sp., showed the highest growth rate in the oil-containing medium as well as the highest biosurfactant production. When this strain was further tested for biodegradation of hydrocarbons, the results showed that it was able to degrade 90 to 100% of low and medium molecular weight hydrocarbons and 60 to 80% of high molecular weight (C35 to C50) hydrocarbons. This study offers many promising leads for future studies of microbial species and their application for the treatment of hydrocarbon contaminated wastewater and soil in the region and in other areas with similar environmental conditions.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00804"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/08/24/main.PMC10300049.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9729631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prospects and challenges of utilizing sugarcane bagasse as a bio-coagulant precursor for water treatment 利用甘蔗渣作为生物混凝剂前体处理水的前景和挑战

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00805

Kingsley O. Iwuozor , Adewale George Adeniyi , Ebuka Chizitere Emenike , Toluwalase Ojeyemi , Abel U. Egbemhenghe , Chika J. Okorie , Bridget Dunoi Ayoku , Oluwaseyi Damilare Saliu

{"title":"Prospects and challenges of utilizing sugarcane bagasse as a bio-coagulant precursor for water treatment","authors":"Kingsley O. Iwuozor , Adewale George Adeniyi , Ebuka Chizitere Emenike , Toluwalase Ojeyemi , Abel U. Egbemhenghe , Chika J. Okorie , Bridget Dunoi Ayoku , Oluwaseyi Damilare Saliu","doi":"10.1016/j.btre.2023.e00805","DOIUrl":"10.1016/j.btre.2023.e00805","url":null,"abstract":"<div>Sugarcane bagasse is an abundant and renewable agricultural waste material generated by the sugar industry worldwide. The use of sugarcane bagasse as a bio-coagulant precursor in water treatment is an eco-friendly and cost-effective approach that has shown great potential. This article reviewed the prospects and challenges of utilizing sugarcane bagasse as a bio-coagulant precursor for water treatment. The article reviewed past studies and explored the properties and chemical composition of sugarcane bagasse and the bioactive compounds that can be extracted from it, as well as their potential coagulation performance in water treatment. It was observed that there are few studies that have been published on the subject. The effectiveness of sugarcane bagasse-based coagulants varies depending on several factors, such as pH, temperature, and water quality parameters. However, the lack of standardization in the production of sugarcane bagasse-based coagulants is a challenge that needs to be addressed. Additionally, the optimization of extraction and processing methods to enhance the effectiveness of sugarcane bagasse-based coagulants needs to be investigated further. In conclusion, the use of sugarcane bagasse as a bio-coagulant precursor holds great promise for the future of sustainable water treatment. The potential for sugarcane bagasse to be used as a bio-coagulant precursor highlights the importance of exploring alternative and sustainable materials for water treatment.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00805"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f1/7d/main.PMC10336157.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9822965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

From purposeless residues to biocomposites: A hyphae made connection 从无目的残留物到生物复合物：菌丝连接

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00807

Isabel Enriquez-Medina , Andres Ceballos Bermudez , Erika Y. Ortiz-Montoya , Carlos Alvarez-Vasco

引用次数: 0

Revolutionizing antiviral therapy with nanobodies: Generation and prospects 纳米体抗病毒治疗的革命性进展：产生和前景

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00803

Mujahed I. Mustafa , Ahmed Mohammed

引用次数: 1

Fortification of Chlorella vulgaris with citrus peel amino acid for improvement biomass and protein quality 柑橘皮氨基酸强化小球藻提高生物量和蛋白质质量

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00806

Zhila Heydari Koochi , Kourosh Ghodrat Jahromi , Gholamreza Kavoosi , Asghar Ramezanian

{"title":"Fortification of Chlorella vulgaris with citrus peel amino acid for improvement biomass and protein quality","authors":"Zhila Heydari Koochi , Kourosh Ghodrat Jahromi , Gholamreza Kavoosi , Asghar Ramezanian","doi":"10.1016/j.btre.2023.e00806","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00806","url":null,"abstract":"<div>The amino acid extract (protein hydrolysate) from various citrus peels was employed as an organic nutrition source for the culture of Chlorella to investigate their effects on the biomass and protein quality of the microalgae. The major amino acids in citrus peels included proline, asparagine, aspartate, alanine, serine, and arginine. The most plentiful amino acids in the Chlorella were alanine, glutamic acid, aspartic acid, glycine, serine, threonine, leucine, proline, lysine, and arginine. Adding the citrus peel amino acid extracts to the Chlorella medium enhanced overall microalgal biomass (more than two folds p < 0.05) and protein content (more than 1.25 fold, p < 0.05). Citrus peel amino acids increase essential amino acids and decrease the non-protein amino acid of Chlorella (p > 0.05). The present research shows that citrus peels have good nutritional quality and could be used for the inexpensive cultivation of Chlorella biomass with potential utility for food application.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00806"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50181592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Study of diversity of mineral-forming bacteria in sabkha mats and sediments of mangrove forest in Qatar 卡塔尔红树林sabkha垫和沉积物中成矿细菌多样性的研究

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00811

Toka Mahmoud Farhat , Zulfa Ali Al Disi , Mohammad Yousaf Ashfaq , Nabil Zouari

{"title":"Study of diversity of mineral-forming bacteria in sabkha mats and sediments of mangrove forest in Qatar","authors":"Toka Mahmoud Farhat , Zulfa Ali Al Disi , Mohammad Yousaf Ashfaq , Nabil Zouari","doi":"10.1016/j.btre.2023.e00811","DOIUrl":"10.1016/j.btre.2023.e00811","url":null,"abstract":"<div>The involvement of microorganisms in carbonate minerals and modern dolomite formation in evaporitic environments occupied with microbial mats (i.e., sabkha) and in mangrove forests is evidenced, while its potential diversity requires further elucidation. Microorganisms can create supersaturated microenvironments facilitating the formation of various carbonate minerals through specific metabolic pathways. This is particularly important in arid environments, where deposition and sedimentary structures can occur. This study investigated the biodiversity of halophilic, heterotrophic, and aerobic mineral-forming bacteria in mangrove forests and living and decaying mats of Qatari sabkha. The diversity study was performed at the protein level using MALDI-TOF mass spectrometry protein profiles combined with principal component analysis (PCA), which revealed a high diversity of isolated strains at the taxonomy and protein profile levels. The diversity of the minerals formed in pure cultures was evidenced by SEM/EDS and XRD analysis. Different types of carbonate minerals (calcium carbonate, magnesium carbonates, and high-magnesium calcites) were formed in pure cultures of the studied strains, which might explain their occurrence in the bulk composition of the sediments from where the strains were isolated. These results illuminate the diversity of biological mineral-formation processes in the extreme environments of Qatari sabkhas and mangroves, explaining the high diversity of minerals in these environments.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00811"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10506043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology 从土壤中分离的乳白色芽孢杆菌菌株OPSA3产洗涤剂稳定淀粉酶的研究；响应面法优化

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00808

Emmanuel Tobechukwu Ugwuoji , Tochukwu Nwamaka T. Nwagu , Lewis Iheanacho Ezeogu

{"title":"Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology","authors":"Emmanuel Tobechukwu Ugwuoji , Tochukwu Nwamaka T. Nwagu , Lewis Iheanacho Ezeogu","doi":"10.1016/j.btre.2023.e00808","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00808","url":null,"abstract":"<div>This study aimed to isolate thermostable, alkaliphilic, and detergent-tolerant amylase-producing bacteria. Pure isolates from environmental samples were screened on a starch-based medium (pH 11), and selected isolates were identified using cultural and molecular techniques. Product optimization studies were conducted, and secreted amylase was partially purified using 40% (w/v) saturation ammonium sulfate at 4 °C. The wash performance of concentrated amylase was analyzed. A novel isolate, Paenibacillus lactis OPSA3, was selected for further studies. The isolate produced amylase optimally when grown on banana peels and soybean extracts, which are agro-wastes. Optimization by Response surface Methodology resulted in a 2.1-fold increase in alkaliphilic amylase production. A 2.46-fold purification was achieved, with an enzyme activity yield of 79.53% and specific activity of 26.19 Umg−1. Wash performance analysis using the amylase supplemented with boiled commercial detergent (kiln®) showed good cleaning efficiency. The amylase has the potential for application as a component of green detergent.</div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00808"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50181187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0