Biotechnology Reports最新文献

{"title":"Secondary metabolite profiling using HR-LCMS, antioxidant and anticancer activity of Bacillus cereus PSMS6 methanolic extract: In silico and in vitro study","authors":"Shalini TS ,&nbsp;Manivel G ,&nbsp;Krishna kumar G ,&nbsp;Prathiviraj Ragothaman ,&nbsp;Rajesh Kannan Velu ,&nbsp;Senthilraja P","doi":"10.1016/j.btre.2024.e00842","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00842","url":null,"abstract":"<div><p>Novel anticancer drugs of natural origin have increased tremendously due to the resistance of multiple chemotherapeutic drugs in breast cancer therapy and their high toxicity with undesirable side effects. The study investigates the bioactivity of secondary metabolites derived from <em>Bacillus cereus</em> PSMS6 isolated from marine soil sediment in the Velar estuary, Parangepattai, Cuddalore district, Tamil Nadu, and India. Strains were isolated and antagonistic activity was screened using the agar well diffusion method. <em>B. cereus</em> PSMS6 exhibited potency, and its crude extract was tested for antioxidant, anticancer, and cytotoxic MTT assay potential. The methanolic extract of <em>B. cereus</em> PSMS6 was analyzed by mass spectrometry HRLC-MS and FT-IR to determine the bioactive compounds. A drug interaction study with the anti-breast cancer protein HER2 was performed by molecular docking analysis. Antioxidant activities were determined using total antioxidant scavenging assay, ABTS and DPPH free radical scavenging assays. The total antioxidant scavenging assay of the crude extract of <em>B. cereus</em> methanol had an IC₅₀ value of 28.33±1.01, in ABTS IC₅₀ value of the extract was 29.00±0.28 and in DPPH the IC₅₀ of the extract was 34.91±0.09. The negative ion compound Palmitoylglycerone phosphate had a LibDock score of 149.487 and the positive ion compound N5-(4-Methoxybenzyl) glutamine had 120.116. These compounds show promising anticancer activity. The current study reported that the bioactive secondary metabolite of <em>B. cereus</em> PSMS6 retains anti-cancer, and antioxidant properties. This is the first report to show the production of the Palmitoylglycerone phosphate metabolite from <em>B. cereus</em> PSMS6.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00842"},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000158/pdfft?md5=616ca192af261c51db9f98b7b20f5273&pid=1-s2.0-S2215017X24000158-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of plant produced VHH antibodies against cobra venom toxins for antivenom therapy","authors":"Sarocha Vitayathikornnasak ,&nbsp;Kaewta Rattanapisit ,&nbsp;Ashwini Malla ,&nbsp;Pipob Suwanchaikasem ,&nbsp;Richard Strasser ,&nbsp;Narach Khorattanakulchai ,&nbsp;Kanokporn Pothisamutyothin ,&nbsp;Wanatchaporn Arunmanee ,&nbsp;Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2024.e00841","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00841","url":null,"abstract":"<div><p>Cobra (<em>Naja kaouthia</em>) venom contains many toxins including α-neurotoxin (αNTX) and phospholipase A2 (PLA2), which can cause neurodegeneration, respiratory failure, and even death. The traditional antivenom derived from animal serum faces many challenges and limitations. Heavy-chain-only antibodies (HCAb), fusing V_HH with human IgG Fc region, offer advantages in tissue penetration, antigen binding, and extended half-life. This research involved the construction and transient expression of two types of V_HH-F_C which are specific to α-Neurotoxin (V_HH-αNTX-F_C) and Phospholipase A2 (V_HH-PLA2-F_C) in <em>Nicotiana benthamiana</em> leaves. The recombinant HCAbs were incubated for up to six days to optimize expression levels followed by purification by affinity chromatography and characterization using LC/Q-TOF mass spectrometry (MS). Purified proteins demonstrated over 92 % sequence coverage and an average mass of around 82 kDa with a high-mannose N-glycan profile. An antigen binding assay showed that the V_HH-αNTX-Fc has a greater ability to bind to crude venom than V_HH-PLA2-Fc.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00841"},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000146/pdfft?md5=118770804465782939c653851a16cfe8&pid=1-s2.0-S2215017X24000146-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140647386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterisation of moderately thermostable diisobutyl phthalate degrading esterase from a Great Artesian Basin Bacillus velezensis NP05","authors":"Brandon Mu ,&nbsp;Pawel Sadowski ,&nbsp;Junior Te'o ,&nbsp;Bharat Patel ,&nbsp;Nayana Pathiraja ,&nbsp;Kevin Dudley","doi":"10.1016/j.btre.2024.e00840","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00840","url":null,"abstract":"<div><p>Phthalate esters are known to be endocrine disrupting chemicals and are documented to pollute environments. Enzymatic degradation of PAEs is a potential bioremedial strategy to manage contamination. Thermostable bioremedial enzymes have advantages in enzyme manufacturing and storage. In this study, we identified, overexpressed, and characterised a moderately thermostable para-nitrobenzyl esterase from whole genome sequencing of a <em>Bacillus velezensis</em> NP05 from the Great Artesian Basin<em>,</em> capable of sequential 2-step hydrolysis of diisobutyl phthalate. The pnbA enzyme has a molecular weight of 55.14 kDa and pI of 5.31. It preferentially degrades para-nitrophenyl butanoate and has an optimal pH of 7–8. The pnbA esterase has an optimal temperature of 55 °C with a half-life of 4 h. Using HPLC we found that pnbA (0.122 U) can hydrolyse 0.83 mM of DIBP within 25 min. Lastly, pnbA is potentially a more economically viable candidate for enzymatic bioremediation of diisobutyl phthalate as a free enzyme.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00840"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000134/pdfft?md5=d146a82ef802ac54b9bc95075c82f54e&pid=1-s2.0-S2215017X24000134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140550345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the biotechnological potential of Acinetobacter soli ANG344B: A novel bacterium for 2-phenylethanol production","authors":"Ana R.S. Bernardino ,&nbsp;Filipa Grosso ,&nbsp;Cristiana A.V. Torres ,&nbsp;Maria A.M. Reis ,&nbsp;Luísa Peixe","doi":"10.1016/j.btre.2024.e00839","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00839","url":null,"abstract":"<div><p>A bacterium, <em>Acinetobacter soli</em> ANG344B, isolated from river water, exhibited an exceptional capacity to produce 2-phenylethanol (2-PE) using L-phenylalanine (L-Phe) as a precursor—a capability typically observed in yeasts rather than bacteria. Bioreactor experiments were conducted to evaluate the production performance, using glucose as the carbon source for cellular growth and L-Phe as the precursor for 2-PE production. Remarkably, <em>A. soli</em> ANG344B achieved a 2-PE concentration of 2.35 ± 0.26 g/L in just 24.5 h of cultivation, exhibiting a global volumetric productivity of 0.10 ± 0.01 g/L.h and a production yield of 0.51 ± 0.01 g_2-PE/g_L-Phe, a result hitherto reported only for yeasts. These findings position <em>A. soli</em> ANG344B as a highly promising microorganism for 2-PE production.</p><p>Whole-genome sequencing of <em>A. soli</em> strain ANG344 revealed a genome size of 3.52 Mb with a GC content of 42.7 %. Utilizing the Rapid Annotation using Subsystem Technology (RAST) server, 3418 coding genes were predicted, including genes coding for enzymes previously associated with the metabolic pathway of 2-PE production in other microorganisms, yet unreported in <em>Acinetobacter</em> species. Through gene mapping, 299 subsystems were identified, exhibiting 30 % subsystem coverage. The whole genome sequence data was submitted to NCBI GeneBank with the BioProject ID PRJNA982713. These draft genome data offer significant potential for exploiting the biotechnological capabilities of <em>A. soli</em> strain ANG344 and for conducting further comparative genomic studies.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00839"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000122/pdfft?md5=0c3109ef2bd5d3e299cf15fc1386fa62&pid=1-s2.0-S2215017X24000122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140540608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis, characterization and study of the application of silver nanoparticle for 4-nitrophenol reduction, and antimicrobial activities","authors":"Mengistu Mulu ,&nbsp;Molla Tefera ,&nbsp;Atnafu Guadie ,&nbsp;K. Basavaiah","doi":"10.1016/j.btre.2024.e00838","DOIUrl":"10.1016/j.btre.2024.e00838","url":null,"abstract":"<div><p>Silver nanoparticles (AgNPs) were synthesized from <em>Vigna unguiculata</em> (L) Walp extracted leaves, and characterized. The UV–Visible spectrum showed a peak between 411 and 415 nm at the Plasmon absorbance of the AgNPs. TEM showed that the size of AgNPs ranged from 5 to 13 nm. It was spherical with an average size of 11.08 nm. The size of AgNPs was 7 ± 6 nm and disperse in water. The AgNPs effectively reduced 4-Nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH₄. The AgNPs exhibited a strong antioxidant and antibacterial activity against Gram-negative bacteria: <em>Escherichia coli</em> (E. coli) and Klebsiella pneumonia and Gram-positive: <em>Bacillus pumilus</em> and <em>Staphylococcus aureus</em>. The average zones of inhibition of AgNPs were: 29 mm for <em>Staphylococcus aureus</em>, 23 mm for <em>Bacillus pumilus</em>, 17 mm for Klebsiella pneumonia and 15 mm for <em>Escherichia coli</em> (E. coli). Thus, AgNPs has exhibted good antibacterial activity compared to antibiotics drug and 4-NP reduction.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00838"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000110/pdfft?md5=0060179eecd2a93cd27ee4c3ba2eade0&pid=1-s2.0-S2215017X24000110-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140279213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances and potential applications for metal-organic framework (MOFs) and MOFs-derived materials: Characterizations and antimicrobial activities","authors":"Muhammad Hubab,&nbsp;Mohammad A. Al-Ghouti","doi":"10.1016/j.btre.2024.e00837","DOIUrl":"10.1016/j.btre.2024.e00837","url":null,"abstract":"<div><p>Microbial infections, particularly those caused by antibiotic-resistant pathogens, pose a critical global health threat. Metal-Organic Frameworks (MOFs), porous crystalline structures built from metal ions and organic linkers, initially developed for gas adsorption, have emerged as promising alternatives to traditional antibiotics. This review, covering research up to 2023, explores the potential of MOFs and MOF-based materials as broad-spectrum antimicrobial agents against bacteria, viruses, fungi, and even parasites. It delves into the historical context of antimicrobial agents, recent advancements in MOF research, and the diverse synthesis techniques employed for their production. Furthermore, the review comprehensively analyzes the mechanisms of action by which MOFs combat various microbial threats. By highlighting the vast potential of MOFs, their diverse synthesis methods, and their effectiveness against various pathogens, this study underscores their potential as a novel solution to the growing challenge of antibiotic resistance.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00837"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000109/pdfft?md5=937c1f96dfde24dc8ffa6a920eb5b8f7&pid=1-s2.0-S2215017X24000109-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140272098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using CO2 level monitoring to adjust the stress conditions of morbidostats","authors":"Kerem Bora","doi":"10.1016/j.btre.2024.e00836","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00836","url":null,"abstract":"<div><p>In a conventional morbidostat, cell growth is monitored by measuring OD, and stress conditions are automatically adjusted using OD values. However, phenomena such as biofilm formation, agglomeration, and the presence of opaque substrates or products can result in inaccurate OD measurements of population size, causing morbidostat systems to fail to adjust stress conditions appropriately.</p><p>This study offers a solution for circumstances where it is impractical to determine vital activity based on OD by developing a novel morbidostat system that adjusts stress conditions based on measurements of exhaust CO₂. As a proof of concept, the adaptation of <em>E. coli</em> ATCC 47076 to 48 °C was performed with two morbidostats using this new strategy. Both populations evolved in the morbidostats were confirmed to grow at 48 °C, a temperature their ancestral strain cannot withstand.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00836"},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000092/pdfft?md5=4f2c287d2400180f0dac06186c8cad81&pid=1-s2.0-S2215017X24000092-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140209114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role and mechanisms of microbes in dichlorodiphenyltrichloroethane (DDT) and its residues bioremediation","authors":"Girma Ebsa ,&nbsp;Birhanu Gizaw ,&nbsp;Mesele Admassie ,&nbsp;Tizazu Degu ,&nbsp;Tesfaye Alemu","doi":"10.1016/j.btre.2024.e00835","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00835","url":null,"abstract":"<div><p>Environmental contamination with dichlorodiphenyltrichloroethane (DDT) has sever effects on the ecosystem worldwide. DDT is a recalcitrant synthetic chemical with high toxicity and lipophilicity. It is also bioaccumulated in the food chain and causes genotoxic, estrogenic, carcinogenic, and mutagenic effects on aquatic organisms and humans. Microbial remediation mechanism and its enzymes are very important for removing DDT from environment. DDT and its main residues dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD) can biodegrade slowly in soil and water. To enhance this process, a number of strategies are proposed, such as bio-attenuation, biostimulation, bioaugmentation and the manipulation of environmental conditions to enhance the activity of microbial enzymes. The addition of organic matter and flooding of the soil enhance DDT degradation. Microbial candidates for DDT remediation include micro-algae, fungi and bacteria. This review provide brief information and recommendation on microbial DDT remediation and its mechanisms.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00835"},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000080/pdfft?md5=07e49d02ec1f2e042ea4b92a975c81c6&pid=1-s2.0-S2215017X24000080-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140180516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill","authors":"I. Zapata-Peñasco ,&nbsp;I.A. Avelino-Jiménez ,&nbsp;J. Mendoza-Pérez ,&nbsp;M. Vázquez Guevara ,&nbsp;M. Gutiérrez-Ladrón de Guevara ,&nbsp;M. Valadez- Martínez ,&nbsp;L. Hernández-Maya ,&nbsp;V. Garibay-Febles ,&nbsp;T. Fregoso-Aguilar ,&nbsp;J. Fonseca-Campos","doi":"10.1016/j.btre.2024.e00834","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00834","url":null,"abstract":"<div><p>The environmental and economic impact of an oil spill can be significant. Biotechnologies applied during a marine oil spill involve bioaugmentation with immobilised or encapsulated indigenous hydrocarbonoclastic species selected under laboratory conditions to improve degradation rates. The environmental factors that act as stressors and impact the effectiveness of hydrocarbon removal are one of the challenges associated with these applications. Understanding how native microbes react to environmental stresses is necessary for effective bioaugmentation. Herein, <em>Micrococcus luteus and M. yunnanensis</em> isolated from a marine oil spill mooring system showed hydrocarbonoclastic activity on Maya crude oil in a short time by means of total petroleum hydrocarbons (TPH) at 144 h: <em>M. luteus</em> up to 98.79 % and <em>M. yunnanensis</em> 97.77 % removal. The assessment of <em>Micrococcus</em> biofilms at different temperature (30 °C and 50 °C), pH (5, 6, 7, 8, 9), salinity (30, 50, 60, 70, 80 g/L), and crude oil concentration (1, 5, 15, 25, 35 %) showed different response to the stressors depending on the strain. According to response surface analysis, the main effect was temperature &gt; salinity &gt; hydrocarbon concentration. The hydrocarbonoclastic biofilm architecture was characterised using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Subtle but significant differences were observed: pili in <em>M. luteus</em> by SEM and the topographical differences measured by AFM Power Spectral Density (PSD) analysis, roughness was higher in <em>M. luteus</em> than in <em>M. yunnanensis.</em> In all three domains of life, the Universal Stress Protein (Usp) is crucial for stress adaptation. Herein, the <em>usp</em>A gene expression was analysed in <em>Micrococcus</em> biofilm under environmental stressors. The <em>usp</em>A expression increased up to 2.5-fold in <em>M. luteus</em> biofilms at 30 °C, and 1.3-fold at 50 °C. The highest <em>usp</em>A expression was recorded in M. <em>yunnanensis</em> biofilms at 50 °C with 2.5 and 3-fold with salinities of 50, 60, and 80 g/L at hydrocarbon concentrations of 15, 25, and 35 %. <em>M. yunnanensis</em> biofilms showed greater resilience than <em>M. luteus</em> biofilms when exposed to harsh environmental stressors. <em>M. yunnanensis</em> biofilms were thicker than <em>M. luteus</em> biofilms. Both biofilm responses to environmental stressors through <em>usp</em>A gene expression were consistent with the behaviours observed in the response surface analyses. The <em>uspA</em> gene is a suitable biomarker for assessing environmental stressors of potential microorganisms for bioremediation of marine oil spills and for biosensing the ecophysiological status of native microbiota in a marine petroleum environment.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00834"},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000079/pdfft?md5=a218bad50a709cf2b28c20ebe1a97ddc&pid=1-s2.0-S2215017X24000079-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140104100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated determination of 8-OHdG in cells and tissue via immunofluorescence using a specially created antibody","authors":"Tobias Jung ,&nbsp;Nicole Findik ,&nbsp;Bianca Hartmann ,&nbsp;Katja Hanack ,&nbsp;Kai Grossmann ,&nbsp;Dirk Roggenbuck ,&nbsp;Marc Wegmann ,&nbsp;René Mantke ,&nbsp;Markus Deckert ,&nbsp;Tilman Grune","doi":"10.1016/j.btre.2024.e00833","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00833","url":null,"abstract":"<div><p>Despite powerful DNA repair systems, oxidative damage/modification to DNA is an inevitable side effect of metabolism, ionizing radiation, lifestyle habits, inflammatory pathologies such as type-2 diabetes or metabolic syndrome, cancer and natural aging.</p><p>One of the most common oxidative DNA modifications is 8-OHdG (8‑hydroxy-2′-deoxyguanosine), which is the most widely used marker in research and clinical diagnostics. 8-OHdG is easily and specifically detectable in various samples such as urine, plasma, cells and tissues via a large variety of methods like ELISA, HPLC, chromatographic methods, and immunochemistry.</p><p>Formed by oxidation of guanine and being representative for the degree of DNA damage, 8-OHdG can be also used as biomarker for risk assessment of various cancers as well as degenerative diseases.</p><p>Here, we present a highly specific, self-developed 8-OHdG antibody in successful comparison to a commercially one, tested in cells (FF95, HCT116, and HT22) and intestinal tissue, focusing on automatized evaluation via fluorescence/confocal microscopy.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00833"},"PeriodicalIF":0.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000067/pdfft?md5=be8a910a99fa043fc09362761dfbb0cc&pid=1-s2.0-S2215017X24000067-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}