Xiang Gao , Xin jing , Jiahong Li , Min Guo , Lei Liu , Zhengke Li , Kaihui Liu , Derui Zhu
{"title":"Exploitation of inland salt lake water by dilution and nutrient enrichment to cultivate Vischeria sp. WL1 (Eustigmatophyceae) for biomass and oil production","authors":"Xiang Gao , Xin jing , Jiahong Li , Min Guo , Lei Liu , Zhengke Li , Kaihui Liu , Derui Zhu","doi":"10.1016/j.btre.2023.e00823","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00823","url":null,"abstract":"<div><p>Salt lakes are significant components of global inland waters. Salt lake (SL) water can provide precious mineral resource for microbial growth. The prospect of utilizing diluted SL water for cultivation of a terrestrial oil-producing microalga <em>Vischeria</em> sp. WL1 was evaluated under laboratory conditions. Based on the detected mineral element composition, the water from Gouchi Salt Lake was diluted 2, 4, 6 and 8 folds and used with supplementation of additional nitrogen, phosphorus and iron (SL<sup>+</sup> water). It was found that 4 folds diluted SL<sup>+</sup> water was most favorable for biomass and oil production. When cultivated in this condition, <em>Vischeria</em> sp. WL1 gained a biomass yield of 0.82 g L<sup>−1</sup> and an oil yield of 0.56 g L<sup>−1</sup> after 24 days of cultivation, which is comparable to the optimum productivity we previously established. In addition, total monounsaturated fatty acid contents (64.4∼68.1 %) of the oils resulted from cultures in diluted SL<sup>+</sup>waters were higher than that in the control (55.5 %). It was also noteworthy that in all these cultures the oil contents (652.0∼681.0 mg g<sup>−1</sup>) accounted for the most of the biomass, which are far more than the protein and starch contents. This study demonstrates the feasibility of using SL water as a cost-effective mineral resource to cultivate microalgae for biomass and oil production.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00823"},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000437/pdfft?md5=741fb232ddd6ffbe17ab32cfbc119773&pid=1-s2.0-S2215017X23000437-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138738944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring the metabolic and antioxidant potential of solergy: Implications for enhanced animal production","authors":"Pamela Olivares-Ferretti , Viviana Chavez , Ekaitz Maguregui , Silvia Jiménez , Octavi Colom , Jorge Parodi","doi":"10.1016/j.btre.2023.e00821","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00821","url":null,"abstract":"<div><p>Cell models are indispensable tools in biotechnology when investigating the functional properties of organic compounds. The emergence of various additives designed to enhance animal production has introduced the need for in-depth evaluations, which are often hindered by the complexities of <em>in vivo</em> testing. In this study, we harnessed cell-based models to scrutinize the impact of Solergy as a regulator of cellular metabolism with a particular focus on its modulation of glycogen and antioxidant effects. Our experiment was designed to include assessments of the influence of Solergy on the viability of both terrestrial and aquatic vertebrate cell models, which revealed the benign nature of Solergy and its lack of adverse effects. Furthermore, we examined the capacity of Solergy to modulate intracellular ATP concentrations and enhance glycogen accumulation. Notably, the antioxidant potential of Solergy and its ability to mitigate cellular aging were evaluated within the same cellular frameworks. The outcomes of our investigation suggest that Solergy is a potent metabolic regulator that elevates cellular activity while exerting an antioxidant effect. Importantly, our study demonstrates that Solergy does not induce changes in membrane oxidation. These findings indicate the potential of using Solergy to regulate glycogen synthesis, intracellular ATP concentrations, and oxidative stress in production animals. The multifaceted effects of this additive, which acts as both a metabolism enhancer and an antioxidant, open doors to the creation of custom diets tailored to meet specific production needs while maintaining stable production parameters.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00821"},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000413/pdfft?md5=016ff2f32db08bf89e52e4482ca3eb6b&pid=1-s2.0-S2215017X23000413-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138582341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Induction of micro-rhizomes for in vitro ginger (Zingiber officinale Rosco) disease-free planting materials regeneration","authors":"Genene Gezahegn , Tileye Feyisa , Yayis Rezene","doi":"10.1016/j.btre.2023.e00820","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00820","url":null,"abstract":"<div><p>Ginger has been a leading spice crop in Ethiopia until ginger bacterial wilt disease interrupted its production. The disease transmission is mainly through infected planting materials and products. Regeneration of healthy planting materials is among the best option to minimize the disease impact. This research aimed to induce and produce disease-free in vitro micro-rhizome in a combination of sucrose, BAP, ammonium nitrate, and silver nitrate. The results from observations indicated that sucrose and BAP were best combinations for induction of disease free micro-rhizomes. Ammonium nitrate and silver nitrate showed lower induction than sucrose and BAP combinations alone. The maximum viable number of micro-rhizome (5.67) and shoot number (10.33) were from 80.0 g/l sucrose and 6.0 mg/l BAP contained medium. Viability test indicated 80 % of the micro-rhizomes were sprouted after one month in soil. The micro-rhizome production potential observed in this experiment can enhance disease-free ginger production.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00820"},"PeriodicalIF":0.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000401/pdfft?md5=c1759ce2110458f4774ec69ab7919784&pid=1-s2.0-S2215017X23000401-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138466503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kervin O. Evans , David L. Compton , Christopher D. Skory , Michael Appell
{"title":"Biophysical characterization of α-glucan nanoparticles encapsulating feruloylated soy glycerides (FSG)","authors":"Kervin O. Evans , David L. Compton , Christopher D. Skory , Michael Appell","doi":"10.1016/j.btre.2023.e00817","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00817","url":null,"abstract":"<div><p>Water insoluble <em>α</em>-glucans that were enzymatically synthesized using glucansucrase that was cloned from <em>Leuconostoc mesenteroides</em> NRRL B-1118 were previously shown to form nanoparticles via high pressure homogenization. These <em>α</em>-glucan nanoparticles were previously shown capable of encapsulating a small hydrophobic molecule. This work demonstrates that the same <em>α</em>-glucan can be formed into nanoparticles that encapsulate feruloylated soy glycerides from modified soybean oil, a product of interest to the cosmetic and skin care industries because of the UV absorbance and antioxidant properties of the feruloyl moiety. It is demonstrated that the feruloylated soy glyceride/<em>α</em>-glucan nanoparticles have distinct size, zeta potential and thermal profiles from that of nanoparticles made from <em>α</em>-glucan alone or feruloylated soy glyceride alone. Thermal analysis also demonstrates the release of feruloylated soy glycerides from the <em>α</em>-glucan nanoparticles.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00817"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000371/pdfft?md5=525ecfb7e76fc645ca899088a354d3e0&pid=1-s2.0-S2215017X23000371-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92135913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Habtamu Terefe , Getnet Yitayih , Getachew G. Mengesha
{"title":"Phytochemicals reduced growth, sporulation and conidial dimensions of Fusarium verticillioides, cause of fumonisin contamination in maize grains","authors":"Habtamu Terefe , Getnet Yitayih , Getachew G. Mengesha","doi":"10.1016/j.btre.2023.e00819","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00819","url":null,"abstract":"<div><p>The objective of the study was to evaluate the effectiveness of methanolic extracts of plants against radial growth and spore dimensions of <em>Fusarium verticillioides</em>. Leaf extracts of 25 plants were tested against the fungus. Of which, thirteen extracts were potent against the fungus and evaluated using food poising technique. Growth was evaluated on PDA medium amended with the extracts at 5 mg ml<sup>–1</sup>. Control treatments included plates without (negative) extracts and with synthetic (positive) fungicide. Spore dimension was determined using PDB. The results showed <em>T. vulgaris</em> extract completely inhibited mycelial growth of the fungus as equivalent as the fungicide. Similarly, <em>G. parviflora, C. citratus, R. officinalis, R. chalepensis</em>, and <em>Agave</em> sp. also recorded growth reductions ranging from 71.04 to 81.35 % at day seven. In addition, extracts of <em>Agave</em> sp., <em>C. citratus</em> and <em>T. vulgaris</em> did not support sporulation. Overall, the results indicate that <em>T. vulgaris</em> extracts could be safe source of bioactive chemicals to control <em>F. verticillioides</em>.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00819"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000395/pdfft?md5=1cc2d77ef7caffec3c8425d3d537fc74&pid=1-s2.0-S2215017X23000395-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92044482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olga Babich , Svetlana Ivanova , Aleksandr Tupitsyn , Aleksandr Vladimirov , Elena Nikolaeva , Archana Tiwari , Ekaterina Budenkova , Egor Kashirskikh , Veronika Anokhova , Philippe Michaud , Stanislav Sukhikh
{"title":"Study of the polysaccharide production by the microalgae C-1509 Nannochloris sp. Naumann","authors":"Olga Babich , Svetlana Ivanova , Aleksandr Tupitsyn , Aleksandr Vladimirov , Elena Nikolaeva , Archana Tiwari , Ekaterina Budenkova , Egor Kashirskikh , Veronika Anokhova , Philippe Michaud , Stanislav Sukhikh","doi":"10.1016/j.btre.2023.e00818","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00818","url":null,"abstract":"<div><p>Biologically active compounds, including polysaccharides isolated from microalgae, have various properties. Although <em>Nannochloropsis</em> spp. have the potential to produce secondary metabolites important for biotechnology, only a small part of the research on these microalgae has focused on their ability to produce polysaccharide fractions. This study aims to evaluate the physicochemical growth factors of <em>Nannochloropsis</em> spp. microalgae, which ensure the maximum accumulation of polysaccharides, as well as to optimize the parameters of polysaccharide extraction. The optimal nutrient medium composition was selected to maximize biomass and polysaccharide accumulation. The significance of selecting the extraction module and extraction temperature regime, as well as the cultivation conditions (temperature and active acidity value) is emphasized. Important chemical components of polysaccharides responsible for their biological activity were identified</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00818"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000383/pdfft?md5=4aa5b341e850250652f19ea74d918eb3&pid=1-s2.0-S2215017X23000383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91963088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iván Solís-Sandí , Sara Cordero-Fuentes , Reinaldo Pereira-Reyes , José Roberto Vega-Baudrit , Diego Batista-Menezes , Gabriela Montes de Oca-Vásquez
{"title":"Optimization of the biosynthesis of silver nanoparticles using bacterial extracts and their antimicrobial potential","authors":"Iván Solís-Sandí , Sara Cordero-Fuentes , Reinaldo Pereira-Reyes , José Roberto Vega-Baudrit , Diego Batista-Menezes , Gabriela Montes de Oca-Vásquez","doi":"10.1016/j.btre.2023.e00816","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00816","url":null,"abstract":"<div><p>In the present study, silver nanoparticles (AgNPs) were biosynthesized using the supernatant and the intracellular extract of <em>Cupriavidus necator, Bacillus megaterium</em>, and <em>Bacillus subtilis</em>. The characterization of the AgNPs was carried out using UV–Vis spectroscopy, FTIR, DLS and TEM. Resazurin microtiter-plate assay was used to determine the antimicrobial action of AgNPs against <em>Escherichia coli.</em> UV–Visible spectra showed peaks between 414 and 460 nm. TEM analysis revealed that the synthesized AgNPs showed mostly spherical shapes. DLS results determined sizes from 20.8 to 118.4 nm. The highest antimicrobial activity was obtained with the AgNPs synthesized with supernatant rather than those using the intracellular extract. Therefore, it was determined that the bacterial species, temperature, pH, and type of extract (supernatant or intracellular) influence the biosynthesis. This synthesis thus offers a simple, environmentally friendly, and low-cost method for the production of AgNPs, which can be used as antibacterial agents.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00816"},"PeriodicalIF":0.0,"publicationDate":"2023-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X2300036X/pdfft?md5=d77dd86f44ffb8e55f3c254c648e6ec8&pid=1-s2.0-S2215017X2300036X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92044481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation, screening and identification of ethanol producing yeasts from Ethiopian fermented beverages","authors":"Dagnew Bitew , Anteneh Tesfaye , Berhanu Andualem","doi":"10.1016/j.btre.2023.e00815","DOIUrl":"10.1016/j.btre.2023.e00815","url":null,"abstract":"<div><p>The growing demand for renewable energy sources such as bioethanol is facing a lack of efficient ethanologenic microbes. This study aimed to isolate and screen ethanologenic yeasts from Ethiopian fermented beverages. A progressive screening and selection approach was employed. Selected isolates were evaluated for bioethanol production using banana peel waste as substrate. A total of 102 isolates were obtained. Sixteen isolates were selected based on their tolerance to stress conditions and carbohydrate fermentation and assimilation capacity. Most found moderately tolerant to 10 %, but slightly tolerant at 15 and 20 % (v/v) ethanol concentration. They yield 15.3 to 20.1 g/L and 9.1 ± 0.6 to 12.9 ± 1.3 g/L ethanol from 2 % (w/v) glucose and 80 g/L banana peel, respectively. Molecular characterization identified them as <em>Saccharomyces cerevisiae</em> strains. Results demonstrate insight about their potential role in the ethanol industry. Optimization of the fermentation conditions is recommended.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00815"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10590766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50158889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nusrat Tazin , Christopher Jordon Lambert , Raheel Samuel , Tamara J. Stevenson , Joshua L. Bonkowsky , Bruce K. Gale
{"title":"Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection","authors":"Nusrat Tazin , Christopher Jordon Lambert , Raheel Samuel , Tamara J. Stevenson , Joshua L. Bonkowsky , Bruce K. Gale","doi":"10.1016/j.btre.2023.e00814","DOIUrl":"10.1016/j.btre.2023.e00814","url":null,"abstract":"<div><p>Electroporation is regularly used to deliver agents into cells, including transgenic materials, but it is not used for mutating zebrafish embryos due to the lack of suitable systems, information on appropriate operating parameters, and the challenges posed by the protective chorion. Here, a novel method for gene delivery in zebrafish embryos was developed by combining microinjection into the space between the chorion and the embryo followed by electroporation. This method eliminates the need for chorion removal and injecting into the space between the chorion and embryo eliminates the need for finding and identifying key cell locations before performing an injection, making the process much simpler and more automatable. We also developed a microfluidic electroporation system and optimized electric pulse parameters for transgenesis of embryos. The study provided a novel method for gene delivery in zebrafish embryos that can be potentially implemented in a high throughput transgenesis or mutagenesis system.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00814"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/32/ac/main.PMC10569972.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aamir Ali Abro , Muhammad Anwar , Muhammad Umer Javwad , Mjie Zhang , Fang Liu , Raimundo Jiménez-Ballesta , Ehab A. A. Salama , Mohamed A. A. Ahmed
{"title":"Morphological and physio-biochemical responses under heat stress in cotton: Overview","authors":"Aamir Ali Abro , Muhammad Anwar , Muhammad Umer Javwad , Mjie Zhang , Fang Liu , Raimundo Jiménez-Ballesta , Ehab A. A. Salama , Mohamed A. A. Ahmed","doi":"10.1016/j.btre.2023.e00813","DOIUrl":"10.1016/j.btre.2023.e00813","url":null,"abstract":"<div><p>Cotton is an important cash crop in addition to being a fiber commodity, and it plays an essential part in the economies of numerous nations. High temperature is the most critical element affecting its yield from fertilization to harvest. The optimal temperature for root formation is 30 C -35 °C; however, root development ends around 40 °C. Increased temperature, in particular, influences different biochemical and physiological processes associated with cotton plant, resulting in low seed cotton production. Many studies in various agroecological zones used various agronomic strategies and contemporary breeding techniques to reduce heat stress and improve cotton productivity. To attain desired traits, cotton breeders should investigate all potential possibilities, such as generating superior cultivars by traditional breeding, employing molecular techniques and transgenic methods, such as using genome editing techniques. The main objective of this review is to provide the recent information on the environmental factors, such as temperature, heat and drought, influence the growth and development, morphology and physio-chemical alteration associated with cotton. Furthermore, recent advancement in cotton breeding to combat the serious threat of drought and heat stress.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"40 ","pages":"Article e00813"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/28/c0/main.PMC10582760.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}