Biotechnology Reports最新文献_第8页

Isolation, identification, and screening of biosurfactant-producing and hydrocarbon-degrading bacteria from oil and gas industrial waste 石油天然气工业废弃物中产生生物表面活性剂和降解碳氢化合物细菌的分离、鉴定和筛选

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00804

S. Al-Marri , H.I. Eldos , M.Y. Ashfaq , S. Saeed , S. Skariah , L. Varghese , Y.A. Mohamoud , A.A. Sultan , M.M. Raja

{"title":"Isolation, identification, and screening of biosurfactant-producing and hydrocarbon-degrading bacteria from oil and gas industrial waste","authors":"S. Al-Marri , H.I. Eldos , M.Y. Ashfaq , S. Saeed , S. Skariah , L. Varghese , Y.A. Mohamoud , A.A. Sultan , M.M. Raja","doi":"10.1016/j.btre.2023.e00804","DOIUrl":"10.1016/j.btre.2023.e00804","url":null,"abstract":"<div><p>Qatar is one of the biggest oil and gas producers in the world, coupled with it is challenging environmental conditions (high average temperature: >40 °C, low annual rainfall: 46.71 mm, and high annual evaporation rate: 2200 mm) harbors diverse microbial communities that are novel and robust, with the potential to biodegrade hydrocarbons. In this study, we collected hydrocarbon contaminated sludge, wastewater and soil samples from oil and gas industries in Qatar. Twenty-six bacterial strains were isolated in the laboratory from these samples using high saline conditions and crude oil as the sole carbon source. A total of 15 different bacterial genera were identified in our study that have not been widely reported in the literature or studied for their usage in the biodegradation of hydrocarbons. Interestingly, some of the bacteria that were identified belonged to the same genus however, demonstrated variable growth rates and biosurfactant production. This indicates the possibility of niche specialization and specific evolution to acquire competitive traits for better survival. The most potent strain EXS14, identified as <em>Marinobacter</em> sp.<em>,</em> showed the highest growth rate in the oil-containing medium as well as the highest biosurfactant production. When this strain was further tested for biodegradation of hydrocarbons, the results showed that it was able to degrade 90 to 100% of low and medium molecular weight hydrocarbons and 60 to 80% of high molecular weight (C35 to C50) hydrocarbons. This study offers many promising leads for future studies of microbial species and their application for the treatment of hydrocarbon contaminated wastewater and soil in the region and in other areas with similar environmental conditions.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00804"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/08/24/main.PMC10300049.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9729631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Prospects and challenges of utilizing sugarcane bagasse as a bio-coagulant precursor for water treatment 利用甘蔗渣作为生物混凝剂前体处理水的前景和挑战

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00805

Kingsley O. Iwuozor , Adewale George Adeniyi , Ebuka Chizitere Emenike , Toluwalase Ojeyemi , Abel U. Egbemhenghe , Chika J. Okorie , Bridget Dunoi Ayoku , Oluwaseyi Damilare Saliu

{"title":"Prospects and challenges of utilizing sugarcane bagasse as a bio-coagulant precursor for water treatment","authors":"Kingsley O. Iwuozor , Adewale George Adeniyi , Ebuka Chizitere Emenike , Toluwalase Ojeyemi , Abel U. Egbemhenghe , Chika J. Okorie , Bridget Dunoi Ayoku , Oluwaseyi Damilare Saliu","doi":"10.1016/j.btre.2023.e00805","DOIUrl":"10.1016/j.btre.2023.e00805","url":null,"abstract":"<div><p>Sugarcane bagasse is an abundant and renewable agricultural waste material generated by the sugar industry worldwide. The use of sugarcane bagasse as a bio-coagulant precursor in water treatment is an eco-friendly and cost-effective approach that has shown great potential. This article reviewed the prospects and challenges of utilizing sugarcane bagasse as a bio-coagulant precursor for water treatment. The article reviewed past studies and explored the properties and chemical composition of sugarcane bagasse and the bioactive compounds that can be extracted from it, as well as their potential coagulation performance in water treatment. It was observed that there are few studies that have been published on the subject. The effectiveness of sugarcane bagasse-based coagulants varies depending on several factors, such as pH, temperature, and water quality parameters. However, the lack of standardization in the production of sugarcane bagasse-based coagulants is a challenge that needs to be addressed. Additionally, the optimization of extraction and processing methods to enhance the effectiveness of sugarcane bagasse-based coagulants needs to be investigated further. In conclusion, the use of sugarcane bagasse as a bio-coagulant precursor holds great promise for the future of sustainable water treatment. The potential for sugarcane bagasse to be used as a bio-coagulant precursor highlights the importance of exploring alternative and sustainable materials for water treatment.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00805"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f1/7d/main.PMC10336157.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9822965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

From purposeless residues to biocomposites: A hyphae made connection 从无目的残留物到生物复合物：菌丝连接

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00807

Isabel Enriquez-Medina , Andres Ceballos Bermudez , Erika Y. Ortiz-Montoya , Carlos Alvarez-Vasco

引用次数: 0

Revolutionizing antiviral therapy with nanobodies: Generation and prospects 纳米体抗病毒治疗的革命性进展：产生和前景

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00803

Mujahed I. Mustafa , Ahmed Mohammed

引用次数: 1

Fortification of Chlorella vulgaris with citrus peel amino acid for improvement biomass and protein quality 柑橘皮氨基酸强化小球藻提高生物量和蛋白质质量

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00806

Zhila Heydari Koochi , Kourosh Ghodrat Jahromi , Gholamreza Kavoosi , Asghar Ramezanian

{"title":"Fortification of Chlorella vulgaris with citrus peel amino acid for improvement biomass and protein quality","authors":"Zhila Heydari Koochi , Kourosh Ghodrat Jahromi , Gholamreza Kavoosi , Asghar Ramezanian","doi":"10.1016/j.btre.2023.e00806","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00806","url":null,"abstract":"<div><p>The amino acid extract (protein hydrolysate) from various citrus peels was employed as an organic nutrition source for the culture of <em>Chlorella</em> to investigate their effects on the biomass and protein quality of the microalgae. The major amino acids in citrus peels included proline, asparagine, aspartate, alanine, serine, and arginine. The most plentiful amino acids in the <em>Chlorella</em> were alanine, glutamic acid, aspartic acid, glycine, serine, threonine, leucine, proline, lysine, and arginine. Adding the citrus peel amino acid extracts to the <em>Chlorella</em> medium enhanced overall microalgal biomass (more than two folds <em>p</em> < 0.05) and protein content (more than 1.25 fold, <em>p</em> < 0.05). Citrus peel amino acids increase essential amino acids and decrease the non-protein amino acid of <em>Chlorella</em> (<em>p</em> > 0.05). The present research shows that citrus peels have good nutritional quality and could be used for the inexpensive cultivation of <em>Chlorella</em> biomass with potential utility for food application.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00806"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50181592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Study of diversity of mineral-forming bacteria in sabkha mats and sediments of mangrove forest in Qatar 卡塔尔红树林sabkha垫和沉积物中成矿细菌多样性的研究

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00811

Toka Mahmoud Farhat , Zulfa Ali Al Disi , Mohammad Yousaf Ashfaq , Nabil Zouari

{"title":"Study of diversity of mineral-forming bacteria in sabkha mats and sediments of mangrove forest in Qatar","authors":"Toka Mahmoud Farhat , Zulfa Ali Al Disi , Mohammad Yousaf Ashfaq , Nabil Zouari","doi":"10.1016/j.btre.2023.e00811","DOIUrl":"10.1016/j.btre.2023.e00811","url":null,"abstract":"<div><p>The involvement of microorganisms in carbonate minerals and modern dolomite formation in evaporitic environments occupied with microbial mats (i.e., sabkha) and in mangrove forests is evidenced, while its potential diversity requires further elucidation. Microorganisms can create supersaturated microenvironments facilitating the formation of various carbonate minerals through specific metabolic pathways. This is particularly important in arid environments, where deposition and sedimentary structures can occur. This study investigated the biodiversity of halophilic, heterotrophic, and aerobic mineral-forming bacteria in mangrove forests and living and decaying mats of Qatari sabkha. The diversity study was performed at the protein level using MALDI-TOF mass spectrometry protein profiles combined with principal component analysis (PCA), which revealed a high diversity of isolated strains at the taxonomy and protein profile levels. The diversity of the minerals formed in pure cultures was evidenced by SEM/EDS and XRD analysis. Different types of carbonate minerals (calcium carbonate, magnesium carbonates, and high-magnesium calcites) were formed in pure cultures of the studied strains, which might explain their occurrence in the bulk composition of the sediments from where the strains were isolated. These results illuminate the diversity of biological mineral-formation processes in the extreme environments of Qatari sabkhas and mangroves, explaining the high diversity of minerals in these environments.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00811"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10506043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology 从土壤中分离的乳白色芽孢杆菌菌株OPSA3产洗涤剂稳定淀粉酶的研究；响应面法优化

Biotechnology Reports Pub Date : 2023-09-01 DOI: 10.1016/j.btre.2023.e00808

Emmanuel Tobechukwu Ugwuoji , Tochukwu Nwamaka T. Nwagu , Lewis Iheanacho Ezeogu

{"title":"Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology","authors":"Emmanuel Tobechukwu Ugwuoji , Tochukwu Nwamaka T. Nwagu , Lewis Iheanacho Ezeogu","doi":"10.1016/j.btre.2023.e00808","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00808","url":null,"abstract":"<div><p>This study aimed to isolate thermostable, alkaliphilic, and detergent-tolerant amylase-producing bacteria. Pure isolates from environmental samples were screened on a starch-based medium (pH 11), and selected isolates were identified using cultural and molecular techniques. Product optimization studies were conducted, and secreted amylase was partially purified using 40% (w/v) saturation ammonium sulfate at 4 °C. The wash performance of concentrated amylase was analyzed. A novel isolate, Paenibacillus lactis OPSA3, was selected for further studies. The isolate produced amylase optimally when grown on banana peels and soybean extracts, which are agro-wastes. Optimization by Response surface Methodology resulted in a 2.1-fold increase in alkaliphilic amylase production. A 2.46-fold purification was achieved, with an enzyme activity yield of 79.53% and specific activity of 26.19 Umg<sup>−1</sup>. Wash performance analysis using the amylase supplemented with boiled commercial detergent (kiln®) showed good cleaning efficiency. The amylase has the potential for application as a component of green detergent.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"39 ","pages":"Article e00808"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50181187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotic therapy, African fermented foods and food-derived bioactive peptides in the management of SARS-CoV-2 cases and other viral infections 益生菌疗法、非洲发酵食品和食品衍生的生物活性肽治疗严重急性呼吸系统综合征冠状病毒2型病例和其他病毒感染

Biotechnology Reports Pub Date : 2023-06-01 DOI: 10.1016/j.btre.2023.e00795

Florence Chioma Mgbodile , Tochukwu Nwamaka T. Nwagu

{"title":"Probiotic therapy, African fermented foods and food-derived bioactive peptides in the management of SARS-CoV-2 cases and other viral infections","authors":"Florence Chioma Mgbodile , Tochukwu Nwamaka T. Nwagu","doi":"10.1016/j.btre.2023.e00795","DOIUrl":"10.1016/j.btre.2023.e00795","url":null,"abstract":"<div><p>The current paper focuses on the impact of probiotics, African fermented foods and bioactive peptides on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection severity and related viral infections. Using probiotics or bioactive peptides as therapeutic adjuncts appears superior to standard care alone. Probiotics play critical roles in innate and adaptive immune modulation by balancing the gut microbiota to combat viral infections, secondary bacterial infections and microbial dysbiosis. African fermented foods contain abundant potential probiotic microorganisms such as the lactic acid bacteria (LAB), Saccharomyces, and Bacillus. More so, fermented food-derived bioactive peptides play vital roles in preventing cardiovascular diseases, hypertension, lung injury, diabetes, and other COVID-19 comorbidities. Regularly incorporating potential probiotics and bioactive peptides into diets should enable a build-up of the benefits in the body system that may result in a better prognosis, especially in COVID-19 patients with underlying complexities. Despite the reported therapeutic potentials of probiotics and fermented foods, numerous setbacks exist regarding their application in disease management. These shortfalls underscore an evident need for more studies to evaluate the specific potentials of probiotics and traditional fermented foods in ameliorating SARS-CoV-2 and other viral infections.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"38 ","pages":"Article e00795"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10066861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9308931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A straightforward microfluidic-based approach toward optimizing transduction efficiency of HIV-1-derived lentiviral vectors in BCP-ALL cells 一种直接的基于微流体的方法优化HIV-1衍生的慢病毒载体在BCP-ALL细胞中的转导效率

Biotechnology Reports Pub Date : 2023-06-01 DOI: 10.1016/j.btre.2023.e00792

Seyed Esmaeil Ahmadi, Rima Manafi Shabestari, Amir Asri kojabad, Majid Safa

{"title":"A straightforward microfluidic-based approach toward optimizing transduction efficiency of HIV-1-derived lentiviral vectors in BCP-ALL cells","authors":"Seyed Esmaeil Ahmadi, Rima Manafi Shabestari, Amir Asri kojabad, Majid Safa","doi":"10.1016/j.btre.2023.e00792","DOIUrl":"10.1016/j.btre.2023.e00792","url":null,"abstract":"<div><h3>Background</h3><p>HIV-1-derived lentiviral vectors (LVs) are capable of transducing human cells by integrating the transgene into the host genome. In order to do that, LVs should have enough time and space to interact with the surface of the target cells. Herein, we used a microfluidic system to facilitate the transduction of BCP-ALL cells.</p></div><div><h3>Methods and Results</h3><p>We used a SU-8 mold to fabricate a PDMS microfluidic chip containing three channels with a 50 μm height and a surface matching 96-well plates. In order to produce LVs, we used HEK293T cells to package the second generation of LVs. First, we evaluated the cell recovery from the microfluidic chip. Cell recovery assessment showcased that 3 h and 6 h of incubation in microfluidic channels containing 100,000 NALM-6 (BCP-ALL) cells with 2μL of culture media yielded 87±7.2% and 80.6 ± 10% of cell recovery, respectively. Afterward, the effects of LV-induced toxicity were evaluated using 10–30% LV concentrations in time frames ranging from 3 h to 24 h. In 96-well plates, it took 12–24 h for the viruses with 20% and 30% concentrations to affect the cell survival significantly. These effects were intensified in the microfluidic system implying that microfluidic is capable of enhancing LV transduction. Based on the evidence of cell recovery and cell survival we chose 6 h of incubation with 20% LV.</p></div><div><h3>Conclusion</h3><p>The results from EGFP expression showcased that a microfluidic system could increase the LV transduction in BCP-ALL cells by almost 9-folds. All in all, the microfluidic system seems to be a great armamentarium in optimizing LV-based transduction.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"38 ","pages":"Article e00792"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9518652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of SARS-CoV-2 neutralizing antibody detection assay by using recombinant plant-produced proteins 利用重组植物产生的蛋白质建立严重急性呼吸系统综合征冠状病毒2型中和抗体检测方法

Biotechnology Reports Pub Date : 2023-06-01 DOI: 10.1016/j.btre.2023.e00796

Perawat Jirarojwattana , Balamurugan Shanmugaraj , Kaewta Rattanapisit , Waranyoo Phoolcharoen

{"title":"Development of SARS-CoV-2 neutralizing antibody detection assay by using recombinant plant-produced proteins","authors":"Perawat Jirarojwattana , Balamurugan Shanmugaraj , Kaewta Rattanapisit , Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2023.e00796","DOIUrl":"10.1016/j.btre.2023.e00796","url":null,"abstract":"<div><p>Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme 2 (ACE2) produced from <em>Nicotiana benthamiana</em> for the development of an affordable neutralizing antibodies detection assay. The results showed that the plant-produced ACE2 can bind to the receptor binding domain (RBD) of the SARS-CoV-2, and was used to develop sVNT with plant-produced RBD protein. The sVNT developed using plant-produced proteins showed high sensitivity and specificity when validated with a group of 30 RBD vaccinated mice sera and the results were correlated with cVNT titer. This preliminary finding suggests that the plants could offer a cost-effective platform for producing diagnostic reagents.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"38 ","pages":"Article e00796"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9e/af/main.PMC10077816.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9679520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1