Biotechnology Reports最新文献

{"title":"Cultivation of Cupriavidus necatorstrains on hydrolyzed lignocellulosic feedstocks widely available in Europe","authors":"Halima Aliyu Alhafiz ,&nbsp;Karin Longus ,&nbsp;Rob A.J. Verlinden ,&nbsp;Vera Lambauer ,&nbsp;Andreas Kruschitz ,&nbsp;Regina Kratzer","doi":"10.1016/j.btre.2025.e00899","DOIUrl":"10.1016/j.btre.2025.e00899","url":null,"abstract":"<div><div>Today, 85 % of the carbon in organic chemicals and their derivatives comes from fossil sources. Replacing fossil-based materials with sustainable sources requires large quantities of feedstocks and mature technologies. Biorefineries based on lignocellulose have great potential to replace fossil raw materials in the short and medium term. Here we want to pave the way for the bacterium <em>Cupriavidus necator</em> as a versatile biotechnological workhorse in future biorefineries. Wheat straw, beech, pine and spruce reflect lignocellulosic biomass from the agricultural waste and wood sectors that is widespread in Europe. Miscanthus was chosen as an emerging energy crop. Lignocellulose feedstocks were pretreated by steam explosion under variable conditions prior to enzymatic hydrolysis. Native <em>Cupriavidus necator</em> and a strain adapted by laboratory evolution were shown to grow on 16 filtered lignocellulosic hydrolysates as the sole carbon source and without prior detoxification.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00899"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seaweed-derived bioactives with anti-tyrosinase activity: a potential for skin-whitening cosmetics with <i>in silico</i> and <i>in vitro</i> approaches.","authors":"Arachaporn Thong-Olran, Supatchar Sermsakulwat, Tiwtawat Napiroon, Phuphiphat Jaikaew, Sumet Kongkiatpaiboon, Ngampuk Tayana, Bongkot Wichachucherd, Theppanya Charoenrat, Thrissawan Traijitt, Supenya Chittapun","doi":"10.1016/j.btre.2025.e00910","DOIUrl":"10.1016/j.btre.2025.e00910","url":null,"abstract":"<p><p>Seaweed is a promising source of bioactive compounds with potential applications in skincare, particularly for addressing hyperpigmentation. This study investigated the chemical profiles and biological activities of hydrophilic and lipophilic extracts from <i>Sargassum polycystum, Caulerpa lentillifera</i>, and <i>Gracilaria fisheri</i>. LC-MS and GC-MS analyses identified 8 and 112 compounds in hydrophilic and lipophilic extracts, respectively. Among these, <i>G. fisheri</i> lipophilic extracts exhibited the strongest anti-tyrosinase activity. Molecular docking identified eight compounds with low binding affinities comparable to kojic acid and tropolone, while ADMET predictions highlighted stigmasterol for its favorable skin permeability and safety. <i>In vitro</i> cytotoxicity assays confirmed its low toxicity (IC₅₀ = 3.38 ± 0.28 µg/mL) with no adverse effects at 0.006 µg/mL. Trypsin stability assays and molecular docking against serine proteases (trypsin and chymotrypsin) showed no significant off-target interactions. These findings suggest stigmasterol as a safe, selective, and effective natural tyrosinase inhibitor for potential use in skin-whitening formulation.</p>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"e00910"},"PeriodicalIF":0.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae: revolutionizing skin repair and enhancement.","authors":"Negin Chinjoo, Abooali Golzary","doi":"10.1016/j.btre.2025.e00911","DOIUrl":"10.1016/j.btre.2025.e00911","url":null,"abstract":"<p><p>Microalgae are attracting growing interest in the cosmetics industry due to their rich content of antioxidants, vitamins, and polyunsaturated fatty acids, which offer anti-aging, anti-inflammatory, and photoprotective benefits. Current research primarily focuses on individual species and isolated bioactive compounds. Several challenges persist, including scalability, cost-effective production, low biomass yields, and regulatory constraints. From an environmental perspective, microalgae can be sustainably cultivated on non-arable land, using recycled water and capturing CO₂, thus reducing carbon emissions. Technological advancements, such as photobioreactor systems, have further enhanced resource efficiency and minimized ecological impact. This review explores multiple microalgal species and their combined bioactive potential for innovative cosmetic formulations. By integrating green extraction techniques and circular economy principles, microalgae-based products can meet rising consumer demand for clean beauty while promoting sustainability. With increasing clinical evidence and ongoing technological progress, microalgae hold significant promise for driving eco-friendly innovation in the cosmetics sector.</p>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"e00911"},"PeriodicalIF":0.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12355925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylene Blue Removal Using Alginate-PVA-Pseudomonas aeruginosa Biocomposite: Kinetics and Biodegradation Studies","authors":"Silvia Abdi Pratama ,&nbsp;Adi Setyo Purnomo ,&nbsp;Asranudin Asranudin","doi":"10.1016/j.btre.2025.e00906","DOIUrl":"10.1016/j.btre.2025.e00906","url":null,"abstract":"<div><div>Methylene blue (MB) is a common synthetic dye used in various industries due to its abundance and cost-effectiveness. However, the presence of this synthetic dye in industrial wastewater has the potential to cause significant effects on the environment and public health when released into soil or water bodies. According to results, biological wastewater treatment has emerged as a promising approach due to its economic feasibility, efficiency, and environmental compatibility. Therefore, this study aims to evaluate the MB removal by <em>Pseudomonas aeruginosa</em> immobilized within alginate–polyvinyl alcohol (PVA) matrices and compare removal performance to that of free (non-immobilized) cells. The results showed that the immobilized <em>P. aeruginosa</em> (Alg-PVA-PA beads) exhibited higher MB removal efficiency (72.52%) compared to free cells (55.52%) and Alg-PVA beads alone (43.12%). Adsorption analysis showed that the removal process followed a pseudo-second-order kinetic model, suggesting chemisorption, and was best described by the Langmuir isotherm, showing monolayer adsorption. Additionally, reusability tests showed that the immobilized biocomposite beads retained over 35% MB removal efficiency after five consecutive cycles, suggesting the sustainability of the beads. XRD, FTIR, and SEM-EDX analysis confirmed the successful entrapment of <em>P. aeruginosa</em> within the Alg-PVA matrices and showed structural changes in the beads following the MB removal process. The results were also supported by LC-QTOF/MS outcomes, which showed metabolites with retention times of 1.17, 1.52, 8.35, and 9.36 min, suggesting the successful degradation of MB.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00906"},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction conditions that promote the effect of glycerol on recombinant protein production in Escherichia coli","authors":"Yoshihiro Ojima ,&nbsp;Hajime Saito ,&nbsp;Shintaro Miyuki ,&nbsp;Koichi Fukunaga ,&nbsp;Terumichi Tsuboi ,&nbsp;Masayuki Azuma","doi":"10.1016/j.btre.2025.e00898","DOIUrl":"10.1016/j.btre.2025.e00898","url":null,"abstract":"<div><div>Proinsulin was expressed by <em>Escherichia coli</em> SHuffle T7 with pET system in minimal medium containing glucose (Glu medium), glucose and glycerol (GluGly medium) or glycerol (Gly medium). With 100 μM IPTG, proinsulin production did not increase with glycerol. In contrast, proinsulin production per medium volume in GluGly and Gly media was approximately 3∼4-fold higher than in Glu medium with 10 μM IPTG. mRNA expression of target protein was higher in GluGly versus Glu medium, indicating that proinsulin production was enhanced by the release of glucose-induced catabolite inhibition. Although proinsulin production did not differ between GluGly and Gly media at 25 h, substrate was consumed quickly in GluGly medium with 1.55±0.12 times higher proinsulin production at 15 h. Productivity, considering the production period, was highest in the GluGly medium. This study shows a mixture of glucose and glycerol is valuable for protein production in <em>E. coli</em> with low IPTG concentrations.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00898"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Montbretia flowers as a source of bioactive crocins: Biotechnology tools and delivery systems","authors":"Lucía Morote ,&nbsp;Cristian Martínez Martínez Fajardo ,&nbsp;María Mondéjar López ,&nbsp;Elena Moreno-Gimenez ,&nbsp;Ángela Rubio-Moraga ,&nbsp;Olivia Costantina Demurtas ,&nbsp;Gianfranco Diretto ,&nbsp;Enrique Niza ,&nbsp;Verónica Aragonés ,&nbsp;Alberto López Jiménez ,&nbsp;José-Antonio Daròs ,&nbsp;Oussama Ahrazem ,&nbsp;Lourdes Gómez-Gómez","doi":"10.1016/j.btre.2025.e00891","DOIUrl":"10.1016/j.btre.2025.e00891","url":null,"abstract":"<div><div>Crocins are potent antioxidants with significant therapeutic potential, exhibiting anti-inflammatory, anticancer, and antidepressant properties. The ornamental plant <em>Crocos-mia x crocosmiiflora</em> is widely cultivated for its aromatic and vibrant flowers. In this study, we identified unique crocins as the primary pigments responsible for the flower's coloration. These metabolites predominantly consist of molecules with eight glucose units, followed by crocins containing six and seven glucose units. To elucidate the mo-lecular mechanisms underlying crocin biosynthesis in <em>C. x crocosmiiflora</em>, tran-scriptomic analysis was performed to identify key carotenoid cleavage dioxygenase (CCD) genes. Using <em>Crocus sativus</em> CsCCD2L gene as a bait, we identified a CCD transcript from the transcriptome data. Phylogenetic analysis revealed that the identified CCD belongs to the CCD2 subfamily, and it was designated as CroCCD2. Functional characterization of CroCCD2 was carried out using bacterial expression systems and <em>Nicotiana benthamiana</em> plants with a virus-mediated expression system. These experiments demonstrated that CroCCD2 efficiently converts the precursor zeaxanthin into crocetin, a key intermediate in crocin biosynthesis. Furthermore, we investigated the bioactivity of crocins and discovered that their anti-inflammatory effects depend on their vehiculation within exosomes or liposomes. This suggests that the transport mechanism is critical for the biological activity of crocins. Our findings highlight the specialization of CCD subfamilies in monocots and dicots for crocin biosynthesis and provide evidence of the anti-inflammatory activity of exosome-transported crocins. This study establishes a foundation for further research into the metabolic network of crocins in <em>C. x crocosmiiflora</em> and suggests that the CroCCD2 gene could be introduced into other crop plants to produce these bioactive apocarotenoids.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00891"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized expression of human interleukin-15 in Nicotiana benthamiana and in vitro assessment of its activity on human keratinocytes","authors":"Chalatorn Charnsatabut ,&nbsp;Pipob Suwanchaikasem ,&nbsp;Kaewta Rattanapisit ,&nbsp;Iksen Iksen ,&nbsp;Varisa Pongrakhananon ,&nbsp;Christine Joy I. Bulaon ,&nbsp;Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2025.e00889","DOIUrl":"10.1016/j.btre.2025.e00889","url":null,"abstract":"<div><div>Human interleukin-15 (hIL-15) is a cytokine essential for immune modulation with therapeutic applications in cancer and chronic wound healing. Although hIL-15 is commercially available, large-scale production studies remain limited. With promising clinical trial results, demand for hIL-15 is expected to rise. Plant expression systems offer a sustainable, low-cost alternative for rapid biopharmaceutical production. In this study, we optimized hIL-15 expression in <em>Nicotiana benthamiana</em> and assessed its physicochemical properties and biological activity. We fused hIL-15 to the Fc domain of human IgG1 for efficient purification. Through optimization of the pre- and post-infiltration conditions, we achieved transient expression and recovery at 4 dpi, yielding 33.8 µg/g fresh weight. Peptide mapping confirmed 97 % overall sequence coverage of the primary structure. Treatment with plant-produced hIL-15-Fc effectively promoted human keratinocyte HaCaT cell proliferation and migration <em>in vitro</em>. These findings demonstrated the potential of plant-based platforms for producing therapeutic recombinant hIL-15 that support wound healing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00889"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cleaner horizons: Exploring advanced technologies for pollution remediation","authors":"Khadija Malik,&nbsp;Ashja Iftikhar,&nbsp;Quratulain Maqsood,&nbsp;Muhammad Rizwan Tariq,&nbsp;Shinawar Waseem Ali","doi":"10.1016/j.btre.2025.e00890","DOIUrl":"10.1016/j.btre.2025.e00890","url":null,"abstract":"<div><div>Soil pollution causes many harmful effects by its contaminants or pollutants, which are known as soil pollutants. They are causing serious problems in plants as well as in humans. By entering into plants, harmful chemicals become part of the food chain. When humans consume contaminated food, it has harmful effects on human health. Pollutants are making soil unfit for living. Many techniques are being used for the remediation of soil pollution. Some are traditional techniques; some are innovative and effective as emerging science and technology are going on. In this review, we have discussed some significant methods, their aspects, and how they are playing their role in the remediation. Biological methods such as living organisms, chemical, and genetic manipulation are modern techniques that are being used for soil pollution remediation. Genetic manipulations sometimes change the enzyme processes, which enhance the whole activity by changing some of the proteins of organisms related to enzymes. Pollution remediation can be done by the process of bio-augmentation, which uses different types of strains of microbes for treatment. As there is an increase in the formation of OH compounds, advanced oxidation technologies are being introduced to treat them. Trace metals and heavy metals are also a big problem for soil pollution, which can be treated by phytoremediation techniques that use many different strategies. Nanoparticles are also being used for the treatment of compounds like nitrates, manganese, arsenic, etc. This review will guide you through the different technologies for soil pollution remediation.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00890"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical-induced variations in callus regenerated Atropa acuminata plants: A study on Ethyl Methanesulfonate effects","authors":"Shabeer Ahmad Dar ,&nbsp;Ishfaq Shafi Khan ,&nbsp;Gousia Majeed ,&nbsp;Sumira Tyub ,&nbsp;Irshad Ahmad Nawchoo ,&nbsp;Azra Nahaid Kamili","doi":"10.1016/j.btre.2025.e00888","DOIUrl":"10.1016/j.btre.2025.e00888","url":null,"abstract":"<div><div><em>Atropa acuminata</em> Royle ex Lindl. a member of the solanaceae family is a critically endangered medicinal herb indigenous to the Himalayan region of Kashmir and revered for its therapeutic and economic significance. Ethyl Methanesulfonate (EMS), a chemical mutagen, is commonly used to induce mutations for plant breeding and research purposes. In this study, callus cultures derived from leaf explants of <em>A. acuminata</em> were cultivated on Murashige and Skoog (MS) medium supplemented with Thidiazuron (0.1 mg/l) and treated with varying concentrations of EMS (0.1%, 1.0%) over defined time intervals. The impact of EMS concentrations and exposure durations, as well as their interactions, on the characteristics of regenerated callus were rigorously examined. The proliferation of shoots exhibited a diminishing trend with escalating EMS concentrations. Additionally, EMS elicited substantial alterations in the morphological and physiological attributes of the regenerated plants. Among the fifteen traits evaluated, mutant lines designated as M10, M11, and M6 displayed the most pronounced variations in morphological and physiological parameters compared to the control. Notably, the mutant line M10 exhibited significantly elevated shoot dry weight, root fresh weight, chlorophyll, and carotenoid contents demonstrating up to 98%, 31%, 348%, and 642% enhancements respectively as compared to the control. These mutant lines hold promise for further exploitation in augmenting physiological characteristics and enhancing the yield and quality of terpene glycosides in <em>A. acuminata</em>.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00888"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizobial, passenger nodule endophytes and phyllosphere bacteria in combination with acyl homoserine lactones enhances the growth and yield of groundnut","authors":"Sivakumar Madhan ,&nbsp;Yuvasri Errakutty Arunan ,&nbsp;Anandham Rangasamy ,&nbsp;Balachandar Dananjeyan ,&nbsp;Johnson Iruthayasamy ,&nbsp;Manimaran Gajendiran ,&nbsp;Krishnamoorthy Ramasamy ,&nbsp;Raghu Rajasekaran ,&nbsp;Vincent Saminathan","doi":"10.1016/j.btre.2025.e00893","DOIUrl":"10.1016/j.btre.2025.e00893","url":null,"abstract":"<div><div>Quorum sensing (QS) mechanisms play an essential role in mediating several signals and plant-bacteria interactions, promoting plant growth. This study demonstrated production of multiple Homoserine lactone (HSL) molecules like C6 HSL, C7 HSL, C8 HSL, 3-Hydroxy-C8-HSL and 3-oxo-C14 HSL in rhizobial and passenger endophytes and phyllospheric bacteria which regulated production of plant growth promoting traits <em>viz.,</em> indole acetic acid and exo-polysaccharide production, biofilm formation, and motility. Quorum quenching (QQ) molecules like salicylic acid, gallic acid, and disalicylic acid impaired these traits, but exogenous addition of QS molecules (C7HSL and 3-oxo-C14 HSL) restored these inhibitory effects of QQ compounds. The pot culture experiment revealed that the treatment involving <em>Methylobacterium populi</em> TMV7–4 or <em>Enterobacter cloacae</em> S23 with salicylic acid, C7HSL and 3-oxo-C14 HSL significantly enhanced plant growth including root length, nodulation, pod formation, soil available nutrients and plant nutrients uptake. In future field validation is required for the use of QS molecules in improving groundnut production.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"46 ","pages":"Article e00893"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}