Biotechnology Reports最新文献

{"title":"Exploring synthesis and applications of green nanoparticles and the role of nanotechnology in wastewater treatment","authors":"Shreya Rathod ,&nbsp;Subham Preetam ,&nbsp;Chetan Pandey ,&nbsp;Sweta Parimita Bera","doi":"10.1016/j.btre.2024.e00830","DOIUrl":"10.1016/j.btre.2024.e00830","url":null,"abstract":"<div><p>Current research endeavours are progressively focussing towards discovering sustainable methods for synthesising eco-friendly materials. In this environment, nanotechnology has emerged as a key frontier, especially in bioremediation and biotechnology. A few areas of nanotechnology including membrane technology, sophisticated oxidation processes, and biosensors. It is possible to create nanoparticles (NPs) via physical, chemical, or biological pathways in a variety of sizes and forms. These days, the investigation of plants as substitutes for NP synthesis methods has drawn a lot of interest. Toxic water contaminants such as methyl blue have been shown to be removed upto 70% by nanoparticles. In our article, we aimed at focussing the environmental sustainability and cost-effectiveness towards the green synthesis of nanoparticles. Furthermore it offers a comprehensive thorough summary of green NP synthesis methods which can be distinguished by their ease of use, financial sustainability, and environmentally favourable utilization of plant extracts. This study highlights how green synthesis methods have the potential to transform manufacturing of NPs while adhering to environmental stewardship principles and resource efficiency.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00830"},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000031/pdfft?md5=31643f62a7ffcc37fb2d22b6cd799bd7&pid=1-s2.0-S2215017X24000031-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139638663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation","authors":"Lior Chatow ,&nbsp;Adi Nudel ,&nbsp;Nadav Eyal ,&nbsp;Tal Lupo ,&nbsp;Silvia Ramirez ,&nbsp;Einat Zelinger ,&nbsp;Iris Nesher ,&nbsp;Richard Boxer","doi":"10.1016/j.btre.2024.e00829","DOIUrl":"10.1016/j.btre.2024.e00829","url":null,"abstract":"<div><p>The activity of the terpenes and Cannabidiol (CBD) against human coronavirus (HCoV) strain OC43 and influenza A (H1N1) was evaluated in human lung fibroblasts (MRC-5 cells). Also, we examined whether these ingredients inhibit pro-inflammatory cytokines in peripheral blood mononuclear cells (PBMC). The tested preparations exhibited both anti-inflammatory and antiviral effects. The combination of terpenes was effective against both HCoV-OC43 and influenza A (H1N1) virus. The addition of CBD improved the antiviral activity in some, but not all cases. This variation in activity may suggest an antiviral mechanism. In addition, there was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect after 72 h, as observed under a microscope. The anti-inflammatory properties of terpenes were demonstrated using a pro-inflammatory cytokine-inhibition assay, which revealed significant cytokine inhibition and enhanced by the addition of CBD.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00829"},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X2400002X/pdfft?md5=404e635427acd679e167f9c45eb8ca09&pid=1-s2.0-S2215017X2400002X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139538634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green and cost-effective biofabrication of copper oxide nanoparticles: Exploring antimicrobial and anticancer applications","authors":"Yemane Tadesse Gebreslassie ,&nbsp;Fisseha Guesh Gebremeskel","doi":"10.1016/j.btre.2024.e00828","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00828","url":null,"abstract":"<div><p>Nanotechnology has made remarkable advancements in recent years, revolutionizing various scientific fields, industries, and research institutions through the utilization of metal and metal oxide nanoparticles. Among these nanoparticles, copper oxide nanoparticles (CuO NPs) have garnered significant attention due to their versatile properties and wide-range applications, particularly, as effective antimicrobial and anticancer agents. CuO NPs can be synthesized using different methods, including physical, chemical, and biological approaches. However, conventional chemical and physical approaches are expensive, resource-intensive, and involve the use of hazardous chemicals, which can pose risks to human health and the environment. In contrast, biological synthesis provides a sustainable and cost-effective alternative by eliminating chemical pollutants and allowing for the production of CuO NPs of tailored sizes and shapes. This comprehensive review focused on the green synthesis of CuO NPs using various biological resources, such as plants, microorganisms, and other biological derivatives. Current knowledge and recent trends in green synthesis methods for CuO NPs are discussed, with a specific emphasis on their biomedical applications, particularly in combating cancer and microbial infections. This review highlights the significant potential of CuO NPs in addressing these diseases. By capitalizing on the advantages of biological synthesis, such as environmental safety and the ability to customize nanoparticle characteristics, CuO NPs have emerged as promising therapeutic agents for a wide range of conditions. This review presents compelling findings, demonstrating the remarkable achievements of biologically synthesized CuO NPs as novel therapeutic agents. Their unique properties and mechanisms enable effective combating against cancer cells and various harmful microbial infections. CuO NPs exhibit potent anticancer activity through diverse mechanisms, including induction of apoptosis, inhibition of angiogenesis, and modulation of signaling pathways. Additionally, their antimicrobial activity manifests through various mechanisms, such as disrupting microbial membranes, generating reactive oxygen species, and interfering with microbial enzymes. This review offers valuable insights into the substantial potential of biologically synthesized CuO NPs as an innovative approach for future therapeutic interventions against cancer and microbial infections.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00828"},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000018/pdfft?md5=d7b6b5b81812aea125c8c23442fe9cd5&pid=1-s2.0-S2215017X24000018-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139549995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermentation of micro- and macroalgae as a way to produce value-added products","authors":"Olga Babich ,&nbsp;Svetlana Ivanova ,&nbsp;Philippe Michaud ,&nbsp;Ekaterina Budenkova ,&nbsp;Egor Kashirskikh ,&nbsp;Veronika Anokhova ,&nbsp;Stanislav Sukhikh","doi":"10.1016/j.btre.2023.e00827","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00827","url":null,"abstract":"<div><p>Fermentation of both microalgae and macroalgae is one of the most efficient methods of obtaining valuable value-added products due to the minimal environmental pollution and the availability of economic benefits, as algae do not require arable land and drift algae and algal bloom biomass are considered waste and must be recycled and their fermentation waste utilized. The compounds found in algae can be effectively used in the fuel, food, cosmetic, and pharmaceutical industries, depending on the type of fermentation used. Products such as methane and hydrogen can be produced by anaerobic digestion and dark fermentation of algae, and lactic acid and its polymers can be produced by lactic acid fermentation of algae. Article aims to provide an overview of the different types potential of micro- and macroalgae fermentation, the advantages and disadvantages of each type considered, and the economic feasibility of algal fermentation for the production of various value-added products.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00827"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000474/pdfft?md5=696d9465f6473694d4c2be73cb48653c&pid=1-s2.0-S2215017X23000474-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139090091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunogenicity of a recombinant plant-produced respiratory syncytial virus F subunit vaccine in mice","authors":"Nuttapat Pisuttinusart ,&nbsp;Balamurugan Shanmugaraj ,&nbsp;Chanya Srisaowakarn ,&nbsp;Chutitorn Ketloy ,&nbsp;Eakachai Prompetchara ,&nbsp;Arunee Thitithanyanont ,&nbsp;Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2023.e00826","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00826","url":null,"abstract":"<div><p>Respiratory syncytial virus (RSV) is a highly infectious respiratory virus that causes serious illness, particularly in young children, elderly people, and those with immunocompromised individuals. RSV infection is the leading cause of infant hospitalization and can lead to serious complications such as pneumonia and bronchiolitis. Currently, there is an RSV vaccine approved exclusively for the elderly population, but no approved vaccine specifically designed for infants or any other age groups. Therefore, it is crucial to continue the development of an RSV vaccine specifically tailored for these populations. In this study, the immunogenicity of the two plant-produced RSV-F Fc fusion proteins (Native construct and structural stabilized construct) were examined to assess them as potential vaccine candidates for RSV. The RSV-F Fc fusion proteins were transiently expressed in <em>Nicotiana benthamiana</em> and purified using protein A affinity column chromatography. The recombinant RSV-F Fc fusion protein was recognized by the monoclonal antibody Motavizumab specific against RSV-F protein. Moreover, the immunogenicity of the two purified RSV-F Fc proteins were evaluated in mice by formulating with different adjuvants. According to our results, the plant-produced RSV-F Fc fusion protein is immunogenic in mice. These preliminary findings, demonstrate the immunogenicity of plant-based RSV-F Fc fusion protein, however, further preclinical studies such as antigen dose and adjuvant optimization, safety, toxicity, and challenge studies in animal models are necessary in order to prove the vaccine efficacy.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00826"},"PeriodicalIF":0.0,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000462/pdfft?md5=64a193f01e70380279931067180d84ce&pid=1-s2.0-S2215017X23000462-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139090092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of Hungarian bauxite residue isolates for biotechnological applications","authors":"Viktória Feigl ,&nbsp;Anna Medgyes-Horváth ,&nbsp;András Kari ,&nbsp;Ádám Török ,&nbsp;Nelli Bombolya ,&nbsp;Zsófia Berkl ,&nbsp;Éva Farkas ,&nbsp;Ildikó Fekete-Kertész","doi":"10.1016/j.btre.2023.e00825","DOIUrl":"10.1016/j.btre.2023.e00825","url":null,"abstract":"<div><p>Bauxite residue (red mud) is considered an extremely alkaline and salty environment for the biota. We present the first attempt to isolate, identify and characterise microbes from Hungarian bauxite residues. Four identified bacterial strains belonged to the Bacilli class, one each to the Actinomycetia, Gammaproteobacteria, and Betaproteobacteria classes, and two to the Alphaproteobacteria class. All three identified fungi strains belonged to the Ascomycota division. Most strains tolerated pH 8–10 and salt content at 5–7% NaCl concentration. <em>Alkalihalobacillus pseudofirmus</em> BRHUB7 and <em>Robertmurraya beringensis</em> BRHUB9 can be considered halophilic and alkalitolerant. <em>Priestia aryabhattai</em> BRHUB2, <em>Penicillium chrysogenum</em> BRHUF1 and <em>Aspergillus</em> sp. BRHUF2 are halo- and alkalitolerant strains. Most strains produced siderophores and extracellular polymeric substances, could mobilise phosphorous, and were cellulose degraders. These strains and their enzymes are possible candidates for biotechnological applications in processes requiring extreme conditions, e.g. bioleaching of critical raw materials and rehabilitation of alkaline waste deposits.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00825"},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000450/pdfft?md5=b29f58e1f4736628350e5cea5992f24b&pid=1-s2.0-S2215017X23000450-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139019222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of exogenous enzymes in feedlot cattle diets: Impacts on physiology, rumen fermentation, digestibility and fatty acid profile in rumen and meat","authors":"Alexandre L. Simon ,&nbsp;Priscila M. Copetti ,&nbsp;Rafael V.P. Lago ,&nbsp;Maksuel G. Vitt ,&nbsp;Aline L. Nascimento ,&nbsp;Luiz Eduardo Lobo e Silva ,&nbsp;Roger Wagner ,&nbsp;Bruna Klein ,&nbsp;Camila Soares Martins ,&nbsp;Gilberto V. Kozloski ,&nbsp;Aleksandro S. Da Silva","doi":"10.1016/j.btre.2023.e00824","DOIUrl":"10.1016/j.btre.2023.e00824","url":null,"abstract":"<div><p>The objective of this study was to evaluate if the inclusion of a blend composed of exogenous enzymes (amylase, protease, cellulase, xylanase and beta glucanase) in the individual and combined form in the feedlot steers diet has benefits on the physiology, rumen fermentation, digestibility and fatty acid profile in rumen and meat. The experiment used 24 animals, divided into 4 treatments, described as: T1-CON, T2-BLEND (0.5 g mixture of enzyme), T3-AMIL (0.5 g alpha-amylase), T4-BLEND+AMIL (0.5 g enzyme blend+ 0.5 g amylase). The concentration of mineral matter was higher in the meat of cattle of T4-BLEND+AMIL. A higher proportion of monounsaturated fatty acids was observed in the T3-AMIL group when compared to the others. The percentage of polyunsaturated fatty acids was higher in the T2-BLEND and T4-BLEND+AMIL compared to the T1-CON. The combination of exogenous enzymes in the diet positively modulate nutritional biomarkers, in addition to benefits in the lipid and oxidative profile meat.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00824"},"PeriodicalIF":0.0,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000449/pdfft?md5=05b7a899bbc5cfd1373300ffeb263634&pid=1-s2.0-S2215017X23000449-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploitation of inland salt lake water by dilution and nutrient enrichment to cultivate Vischeria sp. WL1 (Eustigmatophyceae) for biomass and oil production","authors":"Xiang Gao ,&nbsp;Xin jing ,&nbsp;Jiahong Li ,&nbsp;Min Guo ,&nbsp;Lei Liu ,&nbsp;Zhengke Li ,&nbsp;Kaihui Liu ,&nbsp;Derui Zhu","doi":"10.1016/j.btre.2023.e00823","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00823","url":null,"abstract":"<div><p>Salt lakes are significant components of global inland waters. Salt lake (SL) water can provide precious mineral resource for microbial growth. The prospect of utilizing diluted SL water for cultivation of a terrestrial oil-producing microalga <em>Vischeria</em> sp. WL1 was evaluated under laboratory conditions. Based on the detected mineral element composition, the water from Gouchi Salt Lake was diluted 2, 4, 6 and 8 folds and used with supplementation of additional nitrogen, phosphorus and iron (SL⁺ water). It was found that 4 folds diluted SL⁺ water was most favorable for biomass and oil production. When cultivated in this condition, <em>Vischeria</em> sp. WL1 gained a biomass yield of 0.82 g L⁻¹ and an oil yield of 0.56 g L⁻¹ after 24 days of cultivation, which is comparable to the optimum productivity we previously established. In addition, total monounsaturated fatty acid contents (64.4∼68.1 %) of the oils resulted from cultures in diluted SL⁺waters were higher than that in the control (55.5 %). It was also noteworthy that in all these cultures the oil contents (652.0∼681.0 mg g⁻¹) accounted for the most of the biomass, which are far more than the protein and starch contents. This study demonstrates the feasibility of using SL water as a cost-effective mineral resource to cultivate microalgae for biomass and oil production.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00823"},"PeriodicalIF":0.0,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000437/pdfft?md5=741fb232ddd6ffbe17ab32cfbc119773&pid=1-s2.0-S2215017X23000437-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138738944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the metabolic and antioxidant potential of solergy: Implications for enhanced animal production","authors":"Pamela Olivares-Ferretti ,&nbsp;Viviana Chavez ,&nbsp;Ekaitz Maguregui ,&nbsp;Silvia Jiménez ,&nbsp;Octavi Colom ,&nbsp;Jorge Parodi","doi":"10.1016/j.btre.2023.e00821","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00821","url":null,"abstract":"<div><p>Cell models are indispensable tools in biotechnology when investigating the functional properties of organic compounds. The emergence of various additives designed to enhance animal production has introduced the need for in-depth evaluations, which are often hindered by the complexities of <em>in vivo</em> testing. In this study, we harnessed cell-based models to scrutinize the impact of Solergy as a regulator of cellular metabolism with a particular focus on its modulation of glycogen and antioxidant effects. Our experiment was designed to include assessments of the influence of Solergy on the viability of both terrestrial and aquatic vertebrate cell models, which revealed the benign nature of Solergy and its lack of adverse effects. Furthermore, we examined the capacity of Solergy to modulate intracellular ATP concentrations and enhance glycogen accumulation. Notably, the antioxidant potential of Solergy and its ability to mitigate cellular aging were evaluated within the same cellular frameworks. The outcomes of our investigation suggest that Solergy is a potent metabolic regulator that elevates cellular activity while exerting an antioxidant effect. Importantly, our study demonstrates that Solergy does not induce changes in membrane oxidation. These findings indicate the potential of using Solergy to regulate glycogen synthesis, intracellular ATP concentrations, and oxidative stress in production animals. The multifaceted effects of this additive, which acts as both a metabolism enhancer and an antioxidant, open doors to the creation of custom diets tailored to meet specific production needs while maintaining stable production parameters.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00821"},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000413/pdfft?md5=016ff2f32db08bf89e52e4482ca3eb6b&pid=1-s2.0-S2215017X23000413-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138582341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of micro-rhizomes for in vitro ginger (Zingiber officinale Rosco) disease-free planting materials regeneration","authors":"Genene Gezahegn ,&nbsp;Tileye Feyisa ,&nbsp;Yayis Rezene","doi":"10.1016/j.btre.2023.e00820","DOIUrl":"https://doi.org/10.1016/j.btre.2023.e00820","url":null,"abstract":"<div><p>Ginger has been a leading spice crop in Ethiopia until ginger bacterial wilt disease interrupted its production. The disease transmission is mainly through infected planting materials and products. Regeneration of healthy planting materials is among the best option to minimize the disease impact. This research aimed to induce and produce disease-free in vitro micro-rhizome in a combination of sucrose, BAP, ammonium nitrate, and silver nitrate. The results from observations indicated that sucrose and BAP were best combinations for induction of disease free micro-rhizomes. Ammonium nitrate and silver nitrate showed lower induction than sucrose and BAP combinations alone. The maximum viable number of micro-rhizome (5.67) and shoot number (10.33) were from 80.0 g/l sucrose and 6.0 mg/l BAP contained medium. Viability test indicated 80 % of the micro-rhizomes were sprouted after one month in soil. The micro-rhizome production potential observed in this experiment can enhance disease-free ginger production.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00820"},"PeriodicalIF":0.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X23000401/pdfft?md5=c1759ce2110458f4774ec69ab7919784&pid=1-s2.0-S2215017X23000401-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138466503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}