Biotechnology Reports最新文献_第5页

Prioritizing repurposable drugs for Alzheimer’s disease using network-based analysis with concurrent assessment of Long QT syndrome risk 利用基于网络的分析对阿尔茨海默病的可重复使用药物进行优先排序，同时评估长QT综合征的风险

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-07-29 DOI: 10.1016/j.btre.2025.e00909

Alessio Funari , Enrico De Smaele , Paola Paci , Giulia Fiscon

{"title":"Prioritizing repurposable drugs for Alzheimer’s disease using network-based analysis with concurrent assessment of Long QT syndrome risk","authors":"Alessio Funari , Enrico De Smaele , Paola Paci , Giulia Fiscon","doi":"10.1016/j.btre.2025.e00909","DOIUrl":"10.1016/j.btre.2025.e00909","url":null,"abstract":"<div><div>Alzheimer's disease affects 6.9 million Americans aged 65 and older, a number expected to double by 2060. Eight FDA-approved drugs target Alzheimer's, but no cure is available, and most treatments are symptomatic. Drug repurposing, the use of FDA-approved drugs for new indications, is a promising strategy to address this lack of effective therapies. However, despite prior safety approval, repurposable drugs may still trigger unexpected side-effects in new contexts. This study introduces a network-based approach to minimize side-effect risk in drug repositioning, focusing on QT interval prolongation, a cardiac side-effect observed in Alzheimer's patients treated with acetylcholinesterase inhibitors. The method integrates Mode-of-Action and Random Walk with Restart analyses to identify repositioning candidates while assessing QT-related risk. This strategy identified promising compounds including acamprosate, tolcapone, sitagliptin, and diazoxide, with potential to mitigate disease pathology. Gene set enrichment analysis was used to computationally assess the compounds' ability to reverse disease-related gene expression signatures.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00909"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative analysis of RAS signaling effectors reveals stage-dependent oncogenic patterns in colon adenocarcinoma RAS信号效应的综合分析揭示了结肠腺癌的分期依赖性致癌模式

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-06-23 DOI: 10.1016/j.btre.2025.e00902

Loretta László , Anna Lovrics , Álmos Tilajka , Tamás Takács , László Buday , Virag Vas

{"title":"Integrative analysis of RAS signaling effectors reveals stage-dependent oncogenic patterns in colon adenocarcinoma","authors":"Loretta László , Anna Lovrics , Álmos Tilajka , Tamás Takács , László Buday , Virag Vas","doi":"10.1016/j.btre.2025.e00902","DOIUrl":"10.1016/j.btre.2025.e00902","url":null,"abstract":"<div><div>Cancer rarely results from a single gene defect but emerges from disruptions in complex cellular networks. The Network Medicine perspective guides our investigation of cancer-driving interactions, particularly focusing on RAS signaling pathways that are key mediator for cancer development.</div><div>We analyzed gene expression patterns in colon and lung cancers to identify stage-specific molecular drivers. Using computational modelling combined with patient tissue analysis, we discovered five key genes that are specifically altered in early-stage colon cancer: RAF1, PLCE1, RGL1, RIN1, and GRB7. These genes work as RAS effectors in signaling and can effectively distinguish between normal and cancerous colon tissue.</div><div>Our approach combines network analysis with gene expression studies to understand how RAS signaling disruption contributes to colon cancer development. These findings suggest that targeting early-stage RAS-related changes could offer therapeutic opportunities before cancer becomes more complex and harder to treat.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00902"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of the duckweed biomass and starch production utilizing biogenic MnO and ZnO microparticles 利用生物源MnO和ZnO微颗粒提高浮萍生物量和淀粉产量

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-07-16 DOI: 10.1016/j.btre.2025.e00907

Maryam Anar , Sajjad Kamal Shuvro , Muhammad Farooq Hussain Munis , Masaaki Morikawa

引用次数: 0

Investigation and optimization of DNA isolation efficiency using ferrite-based magnetic nanoparticles 铁氧体基磁性纳米颗粒DNA分离效率的研究与优化

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-06-30 DOI: 10.1016/j.btre.2025.e00904

Tímea B. Gerzsenyi , Ágnes M. Ilosvai , Ferenc Kristály , Lajos Daróczi , Michael C. Owen , Béla Viskolcz , László Vanyorek , Emma Szőri-Dorogházi

{"title":"Investigation and optimization of DNA isolation efficiency using ferrite-based magnetic nanoparticles","authors":"Tímea B. Gerzsenyi , Ágnes M. Ilosvai , Ferenc Kristály , Lajos Daróczi , Michael C. Owen , Béla Viskolcz , László Vanyorek , Emma Szőri-Dorogházi","doi":"10.1016/j.btre.2025.e00904","DOIUrl":"10.1016/j.btre.2025.e00904","url":null,"abstract":"<div><div>DNA isolation is a crucial step in many molecular biological applications for diagnostic and research purposes, like detection of infectious diseases or gene expression studies. However, due to the requirement of toxic reagents in traditional procedures and the high expenses of commercial kits, the use of magnetic MNP-based DNA isolation is becoming more widespread. In this study, different ferrite containing MNPs (MnFe<sub>2</sub>O<sub>4</sub>, MnFe<sub>2</sub>O<sub>4</sub>-NH<sub>2</sub>, MgFe<sub>2</sub>O<sub>4</sub>, MgFe<sub>2</sub>O<sub>4</sub>-NH<sub>2</sub> NiFe<sub>2</sub>O<sub>4</sub>, NiFe<sub>2</sub>O<sub>4</sub>-NH<sub>2</sub>) are examined and compared in their pDNA isolation efficiency. Among the tested nanoparticles, we document the use of NiFe<sub>2</sub>O<sub>4</sub> and its amine-functionalized form for the first time. Three protocols for the isolation of pDNA are optimized for each type of nanoparticle and the best protocol is selected based on the quantity, quality and integrity of the extracted DNA. Plasmid samples extracted with the MNPs are transformed into competent bacterial cells and further tests are performed to recover genomic DNA from bacterial cells, leading to the development of another protocol. Bacteria-spiked blood serum samples are produced to extract DNA from a more complex biological matrix.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00904"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scale-down optimization of a robust, parallelizable human induced pluripotent stem cell bioprocess for high-throughput research 用于高通量研究的稳健、可并行的人诱导多能干细胞生物过程的缩小优化

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-05-22 DOI: 10.1016/j.btre.2025.e00900

James Colter , Tiffany Dang , Julia Malinovska , Jessica May Corpuz , Dora Modrcin , Roman Krawetz , Kartikeya Murari , Michael Scott Kallos

{"title":"Scale-down optimization of a robust, parallelizable human induced pluripotent stem cell bioprocess for high-throughput research","authors":"James Colter , Tiffany Dang , Julia Malinovska , Jessica May Corpuz , Dora Modrcin , Roman Krawetz , Kartikeya Murari , Michael Scott Kallos","doi":"10.1016/j.btre.2025.e00900","DOIUrl":"10.1016/j.btre.2025.e00900","url":null,"abstract":"<div><div>Human induced pluripotent stem cell (hiPSC) derived therapeutics require clinically relevant quantities of high-quality cell populations for applications in regenerative medicine. The lack of efficacy exhibited across clinical trials suggests deeper understanding of the networks governing phenotype is needed. Further, costs limit study throughput in characterizing the artificial niche relative to outcomes. We present herein an optimized strategy to enable high-throughput hiPSC expansion at <20 mL research scale. We assessed viability of single cell inoculation and aggregate preformation to facilitate proliferation. We modeled aggregate characteristics against agitation rate. Our results demonstrate tunable control with fold expansion comparable to commercial systems. Marker quantification and teratoma assay confirm functional pluripotency. This approach constitutes a scalable protocol to accelerate hiPSC research, and a significant step in advancing the rate of progress in elucidating links to derivative functionality. This work will enable statistically rigorous studies targeting hiPSC and downstream phenotype for clinical manufacturing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00900"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144168992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green nanoscience for healthcare: Advancing biomedical innovation through eco-synthesized nanoparticle 绿色医疗纳米科学：通过生态合成纳米颗粒推进生物医学创新

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-08-12 DOI: 10.1016/j.btre.2025.e00913

Anjuman Ayub , Atif Khurshid Wani , Suhaib Mohd Malik , Mehvish Ayub , Reena Singh , Chirag Chopra , Tabarak Malik

{"title":"Green nanoscience for healthcare: Advancing biomedical innovation through eco-synthesized nanoparticle","authors":"Anjuman Ayub , Atif Khurshid Wani , Suhaib Mohd Malik , Mehvish Ayub , Reena Singh , Chirag Chopra , Tabarak Malik","doi":"10.1016/j.btre.2025.e00913","DOIUrl":"10.1016/j.btre.2025.e00913","url":null,"abstract":"<div><div>Green synthesis is an eco-friendly and sustainable approach to nanoparticle production using biological sources such as plant extracts and microorganisms. Unlike traditional chemical methods, it aligns with green chemistry principles by reducing toxic reagents, minimizing waste, and lowering environmental impact. Green-synthesized nanoparticles have shown great potential, especially in biomedicine, for targeted drug delivery, antimicrobial treatments, and imaging applications. Their properties can be finely tuned by controlling size, shape, and composition, supporting applications across electronics, healthcare, and environmental engineering. Characterization techniques are essential for analyzing their structural and functional attributes. However, challenges remain in terms of scalability, reproducibility, and lack of standardized synthesis protocols. The aim of this review is to explore recent advances in green nanoparticle synthesis, evaluate key mechanisms, highlight biomedical and environmental applications, and discuss current limitations. The review also emphasizes future directions and the need for interdisciplinary collaboration to unlock the full potential of green nanotechnology.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00913"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction notice to “Fertilization value of municipal sewage sludge for Eucalyptus camaldulensis plants” [Biotechnology Reports 13 (2017) 8–12] 关于“城市污水污泥对桉植物的施肥价值”的撤回通知[生物技术报告13 (2017)8-12]

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-02-27 DOI: 10.1016/j.btre.2025.e00884

Soudani Leila , Maatoug Mhamed , Heilmeier Hermann , Kharytonov Mykola , Wiche Oliver , Moschner Christin , Onyshchenkoc Elena , Bouchenafa Nadia

引用次数: 0

Quorum quenching: A key biological activity of marine actinobacteria extracts for acne vulgaris control 群体猝灭：海洋放线菌提取物控制寻常痤疮的关键生物活性

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-06-24 DOI: 10.1016/j.btre.2025.e00903

María Clara De La Hoz-Romo , Luis Díaz , Javier Gómez-León , Luis Felipe Reyes , Marynes Quintero , Luisa Villamil

{"title":"Quorum quenching: A key biological activity of marine actinobacteria extracts for acne vulgaris control","authors":"María Clara De La Hoz-Romo , Luis Díaz , Javier Gómez-León , Luis Felipe Reyes , Marynes Quintero , Luisa Villamil","doi":"10.1016/j.btre.2025.e00903","DOIUrl":"10.1016/j.btre.2025.e00903","url":null,"abstract":"<div><div>Quorum sensing (QS) regulates virulence factors in bacteria associated with acne vulgaris. Consequently, extracts from marine actinobacteria sourced from Colombian Caribbean sponges and octocorals were screened for quorum quenching (QQ) and anti-biofilm activity. The most promising extract, G12.218 from <em>Promicromonospora</em> sp. CLIVUS-G12218, inhibited QS by 97 % via AI-2 signaling in <em>Vibrio harveyi</em> BB170 and reduced <em>Staphylococcus aureus</em> biofilm by 60 % at 3 mg/L, without cytotoxicity to keratinocytes and fibroblasts.</div><div>Untargeted metabolomics of QQ active and non-active extracts revealed distinct profiles enriched in terpenoids, phenols, and butenolides, potentially linked to bioactivity. These extracts also exhibited antioxidant capacity (18 ± 0.23 µmol TE/L) and hyaluronidase inhibition up to 72.8 %, suggesting skin-protective properties. These findings highlight the potential of marine actinobacteria as a source of QQ and anti-biofilm agents for acne therapy and skincare.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00903"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing cell characterization with microfluidics and AI: a comprehensive review of mechanical, electrical, and hybrid techniques 用微流体和人工智能增强细胞表征：机械、电气和混合技术的综合综述

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-07-22 DOI: 10.1016/j.btre.2025.e00905

Marwan Taha , Anna-Maria Pappa , Hani Saleh , Anas Alazzam

引用次数: 0

Optimization of levan biosynthesis from Bacillus siamensis using batch and continuous stirred-tank bioreactors: A response surface methodology approach 间歇式和连续式搅拌槽生物反应器优化暹逻芽孢杆菌合成levan：响应面法

Biotechnology Reports Pub Date : 2025-09-01 Epub Date: 2025-07-29 DOI: 10.1016/j.btre.2025.e00908

Pongtorn Phengnoi , Nuttinee Teerakulkittipong , Kosin Teeparuksapun , Gary Antonio Lirio , Witawat Jangiam

{"title":"Optimization of levan biosynthesis from Bacillus siamensis using batch and continuous stirred-tank bioreactors: A response surface methodology approach","authors":"Pongtorn Phengnoi , Nuttinee Teerakulkittipong , Kosin Teeparuksapun , Gary Antonio Lirio , Witawat Jangiam","doi":"10.1016/j.btre.2025.e00908","DOIUrl":"10.1016/j.btre.2025.e00908","url":null,"abstract":"<div><div>Levan, a promising fructan polysaccharide for biopharmaceuticals, has limited large-scale production studies. This research optimized and scaled up levan biosynthesis from <em>Bacillus siamensis</em> in continuous stirred-tank bioreactors based on response surface methodology (RSM). Batch cultures optimized for 30 % (w/v) sucrose, pH 5.0, and 48 h incubation yielded a maximum 15.74 % (w/v) levan. The optimal batch conditions were evaluated in a continuous stirred-tank bioreactor, where dilution rates and mixing speeds were examined. At a dilution rate of 0.021 h⁻¹ and an agitation speed of 200 rpm, the maximum productivity was 17.96 % (w/v), and steady-state conditions were attained after three days of continuous fermentation. X-ray diffraction confirmed the amorphous nature of the levan, ideal for biomaterial applications. These results underline the potential of <em>B. siamensis</em> for high-yield levan production and provide a systematic approach for bioprocess parameter optimization, serving as a strong basis for its increased application in industrialized polysaccharide-based bioprocessing.</div></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"47 ","pages":"Article e00908"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0