Biotechnology Reports最新文献

{"title":"Identification and characterisation of moderately thermostable diisobutyl phthalate degrading esterase from a Great Artesian Basin Bacillus velezensis NP05","authors":"Brandon Mu ,&nbsp;Pawel Sadowski ,&nbsp;Junior Te'o ,&nbsp;Bharat Patel ,&nbsp;Nayana Pathiraja ,&nbsp;Kevin Dudley","doi":"10.1016/j.btre.2024.e00840","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00840","url":null,"abstract":"<div><p>Phthalate esters are known to be endocrine disrupting chemicals and are documented to pollute environments. Enzymatic degradation of PAEs is a potential bioremedial strategy to manage contamination. Thermostable bioremedial enzymes have advantages in enzyme manufacturing and storage. In this study, we identified, overexpressed, and characterised a moderately thermostable para-nitrobenzyl esterase from whole genome sequencing of a <em>Bacillus velezensis</em> NP05 from the Great Artesian Basin<em>,</em> capable of sequential 2-step hydrolysis of diisobutyl phthalate. The pnbA enzyme has a molecular weight of 55.14 kDa and pI of 5.31. It preferentially degrades para-nitrophenyl butanoate and has an optimal pH of 7–8. The pnbA esterase has an optimal temperature of 55 °C with a half-life of 4 h. Using HPLC we found that pnbA (0.122 U) can hydrolyse 0.83 mM of DIBP within 25 min. Lastly, pnbA is potentially a more economically viable candidate for enzymatic bioremediation of diisobutyl phthalate as a free enzyme.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00840"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000134/pdfft?md5=d146a82ef802ac54b9bc95075c82f54e&pid=1-s2.0-S2215017X24000134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140550345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the biotechnological potential of Acinetobacter soli ANG344B: A novel bacterium for 2-phenylethanol production","authors":"Ana R.S. Bernardino ,&nbsp;Filipa Grosso ,&nbsp;Cristiana A.V. Torres ,&nbsp;Maria A.M. Reis ,&nbsp;Luísa Peixe","doi":"10.1016/j.btre.2024.e00839","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00839","url":null,"abstract":"<div><p>A bacterium, <em>Acinetobacter soli</em> ANG344B, isolated from river water, exhibited an exceptional capacity to produce 2-phenylethanol (2-PE) using L-phenylalanine (L-Phe) as a precursor—a capability typically observed in yeasts rather than bacteria. Bioreactor experiments were conducted to evaluate the production performance, using glucose as the carbon source for cellular growth and L-Phe as the precursor for 2-PE production. Remarkably, <em>A. soli</em> ANG344B achieved a 2-PE concentration of 2.35 ± 0.26 g/L in just 24.5 h of cultivation, exhibiting a global volumetric productivity of 0.10 ± 0.01 g/L.h and a production yield of 0.51 ± 0.01 g_2-PE/g_L-Phe, a result hitherto reported only for yeasts. These findings position <em>A. soli</em> ANG344B as a highly promising microorganism for 2-PE production.</p><p>Whole-genome sequencing of <em>A. soli</em> strain ANG344 revealed a genome size of 3.52 Mb with a GC content of 42.7 %. Utilizing the Rapid Annotation using Subsystem Technology (RAST) server, 3418 coding genes were predicted, including genes coding for enzymes previously associated with the metabolic pathway of 2-PE production in other microorganisms, yet unreported in <em>Acinetobacter</em> species. Through gene mapping, 299 subsystems were identified, exhibiting 30 % subsystem coverage. The whole genome sequence data was submitted to NCBI GeneBank with the BioProject ID PRJNA982713. These draft genome data offer significant potential for exploiting the biotechnological capabilities of <em>A. soli</em> strain ANG344 and for conducting further comparative genomic studies.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00839"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000122/pdfft?md5=0c3109ef2bd5d3e299cf15fc1386fa62&pid=1-s2.0-S2215017X24000122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140540608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis, characterization and study of the application of silver nanoparticle for 4-nitrophenol reduction, and antimicrobial activities","authors":"Mengistu Mulu ,&nbsp;Molla Tefera ,&nbsp;Atnafu Guadie ,&nbsp;K. Basavaiah","doi":"10.1016/j.btre.2024.e00838","DOIUrl":"10.1016/j.btre.2024.e00838","url":null,"abstract":"<div><p>Silver nanoparticles (AgNPs) were synthesized from <em>Vigna unguiculata</em> (L) Walp extracted leaves, and characterized. The UV–Visible spectrum showed a peak between 411 and 415 nm at the Plasmon absorbance of the AgNPs. TEM showed that the size of AgNPs ranged from 5 to 13 nm. It was spherical with an average size of 11.08 nm. The size of AgNPs was 7 ± 6 nm and disperse in water. The AgNPs effectively reduced 4-Nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH₄. The AgNPs exhibited a strong antioxidant and antibacterial activity against Gram-negative bacteria: <em>Escherichia coli</em> (E. coli) and Klebsiella pneumonia and Gram-positive: <em>Bacillus pumilus</em> and <em>Staphylococcus aureus</em>. The average zones of inhibition of AgNPs were: 29 mm for <em>Staphylococcus aureus</em>, 23 mm for <em>Bacillus pumilus</em>, 17 mm for Klebsiella pneumonia and 15 mm for <em>Escherichia coli</em> (E. coli). Thus, AgNPs has exhibted good antibacterial activity compared to antibiotics drug and 4-NP reduction.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00838"},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000110/pdfft?md5=0060179eecd2a93cd27ee4c3ba2eade0&pid=1-s2.0-S2215017X24000110-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140279213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances and potential applications for metal-organic framework (MOFs) and MOFs-derived materials: Characterizations and antimicrobial activities","authors":"Muhammad Hubab,&nbsp;Mohammad A. Al-Ghouti","doi":"10.1016/j.btre.2024.e00837","DOIUrl":"10.1016/j.btre.2024.e00837","url":null,"abstract":"<div><p>Microbial infections, particularly those caused by antibiotic-resistant pathogens, pose a critical global health threat. Metal-Organic Frameworks (MOFs), porous crystalline structures built from metal ions and organic linkers, initially developed for gas adsorption, have emerged as promising alternatives to traditional antibiotics. This review, covering research up to 2023, explores the potential of MOFs and MOF-based materials as broad-spectrum antimicrobial agents against bacteria, viruses, fungi, and even parasites. It delves into the historical context of antimicrobial agents, recent advancements in MOF research, and the diverse synthesis techniques employed for their production. Furthermore, the review comprehensively analyzes the mechanisms of action by which MOFs combat various microbial threats. By highlighting the vast potential of MOFs, their diverse synthesis methods, and their effectiveness against various pathogens, this study underscores their potential as a novel solution to the growing challenge of antibiotic resistance.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00837"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000109/pdfft?md5=937c1f96dfde24dc8ffa6a920eb5b8f7&pid=1-s2.0-S2215017X24000109-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140272098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using CO2 level monitoring to adjust the stress conditions of morbidostats","authors":"Kerem Bora","doi":"10.1016/j.btre.2024.e00836","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00836","url":null,"abstract":"<div><p>In a conventional morbidostat, cell growth is monitored by measuring OD, and stress conditions are automatically adjusted using OD values. However, phenomena such as biofilm formation, agglomeration, and the presence of opaque substrates or products can result in inaccurate OD measurements of population size, causing morbidostat systems to fail to adjust stress conditions appropriately.</p><p>This study offers a solution for circumstances where it is impractical to determine vital activity based on OD by developing a novel morbidostat system that adjusts stress conditions based on measurements of exhaust CO₂. As a proof of concept, the adaptation of <em>E. coli</em> ATCC 47076 to 48 °C was performed with two morbidostats using this new strategy. Both populations evolved in the morbidostats were confirmed to grow at 48 °C, a temperature their ancestral strain cannot withstand.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00836"},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000092/pdfft?md5=4f2c287d2400180f0dac06186c8cad81&pid=1-s2.0-S2215017X24000092-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140209114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role and mechanisms of microbes in dichlorodiphenyltrichloroethane (DDT) and its residues bioremediation","authors":"Girma Ebsa ,&nbsp;Birhanu Gizaw ,&nbsp;Mesele Admassie ,&nbsp;Tizazu Degu ,&nbsp;Tesfaye Alemu","doi":"10.1016/j.btre.2024.e00835","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00835","url":null,"abstract":"<div><p>Environmental contamination with dichlorodiphenyltrichloroethane (DDT) has sever effects on the ecosystem worldwide. DDT is a recalcitrant synthetic chemical with high toxicity and lipophilicity. It is also bioaccumulated in the food chain and causes genotoxic, estrogenic, carcinogenic, and mutagenic effects on aquatic organisms and humans. Microbial remediation mechanism and its enzymes are very important for removing DDT from environment. DDT and its main residues dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD) can biodegrade slowly in soil and water. To enhance this process, a number of strategies are proposed, such as bio-attenuation, biostimulation, bioaugmentation and the manipulation of environmental conditions to enhance the activity of microbial enzymes. The addition of organic matter and flooding of the soil enhance DDT degradation. Microbial candidates for DDT remediation include micro-algae, fungi and bacteria. This review provide brief information and recommendation on microbial DDT remediation and its mechanisms.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00835"},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000080/pdfft?md5=07e49d02ec1f2e042ea4b92a975c81c6&pid=1-s2.0-S2215017X24000080-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140180516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental stressor assessment of hydrocarbonoclastic bacteria biofilms from a marine oil spill","authors":"I. Zapata-Peñasco ,&nbsp;I.A. Avelino-Jiménez ,&nbsp;J. Mendoza-Pérez ,&nbsp;M. Vázquez Guevara ,&nbsp;M. Gutiérrez-Ladrón de Guevara ,&nbsp;M. Valadez- Martínez ,&nbsp;L. Hernández-Maya ,&nbsp;V. Garibay-Febles ,&nbsp;T. Fregoso-Aguilar ,&nbsp;J. Fonseca-Campos","doi":"10.1016/j.btre.2024.e00834","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00834","url":null,"abstract":"<div><p>The environmental and economic impact of an oil spill can be significant. Biotechnologies applied during a marine oil spill involve bioaugmentation with immobilised or encapsulated indigenous hydrocarbonoclastic species selected under laboratory conditions to improve degradation rates. The environmental factors that act as stressors and impact the effectiveness of hydrocarbon removal are one of the challenges associated with these applications. Understanding how native microbes react to environmental stresses is necessary for effective bioaugmentation. Herein, <em>Micrococcus luteus and M. yunnanensis</em> isolated from a marine oil spill mooring system showed hydrocarbonoclastic activity on Maya crude oil in a short time by means of total petroleum hydrocarbons (TPH) at 144 h: <em>M. luteus</em> up to 98.79 % and <em>M. yunnanensis</em> 97.77 % removal. The assessment of <em>Micrococcus</em> biofilms at different temperature (30 °C and 50 °C), pH (5, 6, 7, 8, 9), salinity (30, 50, 60, 70, 80 g/L), and crude oil concentration (1, 5, 15, 25, 35 %) showed different response to the stressors depending on the strain. According to response surface analysis, the main effect was temperature &gt; salinity &gt; hydrocarbon concentration. The hydrocarbonoclastic biofilm architecture was characterised using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Subtle but significant differences were observed: pili in <em>M. luteus</em> by SEM and the topographical differences measured by AFM Power Spectral Density (PSD) analysis, roughness was higher in <em>M. luteus</em> than in <em>M. yunnanensis.</em> In all three domains of life, the Universal Stress Protein (Usp) is crucial for stress adaptation. Herein, the <em>usp</em>A gene expression was analysed in <em>Micrococcus</em> biofilm under environmental stressors. The <em>usp</em>A expression increased up to 2.5-fold in <em>M. luteus</em> biofilms at 30 °C, and 1.3-fold at 50 °C. The highest <em>usp</em>A expression was recorded in M. <em>yunnanensis</em> biofilms at 50 °C with 2.5 and 3-fold with salinities of 50, 60, and 80 g/L at hydrocarbon concentrations of 15, 25, and 35 %. <em>M. yunnanensis</em> biofilms showed greater resilience than <em>M. luteus</em> biofilms when exposed to harsh environmental stressors. <em>M. yunnanensis</em> biofilms were thicker than <em>M. luteus</em> biofilms. Both biofilm responses to environmental stressors through <em>usp</em>A gene expression were consistent with the behaviours observed in the response surface analyses. The <em>uspA</em> gene is a suitable biomarker for assessing environmental stressors of potential microorganisms for bioremediation of marine oil spills and for biosensing the ecophysiological status of native microbiota in a marine petroleum environment.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00834"},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000079/pdfft?md5=a218bad50a709cf2b28c20ebe1a97ddc&pid=1-s2.0-S2215017X24000079-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140104100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated determination of 8-OHdG in cells and tissue via immunofluorescence using a specially created antibody","authors":"Tobias Jung ,&nbsp;Nicole Findik ,&nbsp;Bianca Hartmann ,&nbsp;Katja Hanack ,&nbsp;Kai Grossmann ,&nbsp;Dirk Roggenbuck ,&nbsp;Marc Wegmann ,&nbsp;René Mantke ,&nbsp;Markus Deckert ,&nbsp;Tilman Grune","doi":"10.1016/j.btre.2024.e00833","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00833","url":null,"abstract":"<div><p>Despite powerful DNA repair systems, oxidative damage/modification to DNA is an inevitable side effect of metabolism, ionizing radiation, lifestyle habits, inflammatory pathologies such as type-2 diabetes or metabolic syndrome, cancer and natural aging.</p><p>One of the most common oxidative DNA modifications is 8-OHdG (8‑hydroxy-2′-deoxyguanosine), which is the most widely used marker in research and clinical diagnostics. 8-OHdG is easily and specifically detectable in various samples such as urine, plasma, cells and tissues via a large variety of methods like ELISA, HPLC, chromatographic methods, and immunochemistry.</p><p>Formed by oxidation of guanine and being representative for the degree of DNA damage, 8-OHdG can be also used as biomarker for risk assessment of various cancers as well as degenerative diseases.</p><p>Here, we present a highly specific, self-developed 8-OHdG antibody in successful comparison to a commercially one, tested in cells (FF95, HCT116, and HT22) and intestinal tissue, focusing on automatized evaluation via fluorescence/confocal microscopy.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00833"},"PeriodicalIF":0.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000067/pdfft?md5=be8a910a99fa043fc09362761dfbb0cc&pid=1-s2.0-S2215017X24000067-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and antiproliferative activity of enzymatic hydrolysates from red tilapia (Oreochromis spp.) viscera","authors":"José E. Zapata ,&nbsp;Leidy J. Gómez-Sampedro","doi":"10.1016/j.btre.2024.e00832","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00832","url":null,"abstract":"<div><p>The antioxidant and antiproliferative activity of red tilapia (<em>Oreochromis</em> spp.) viscera hydrolysates (RTVH) was evaluated. For that, the hydrolysates was applied to three cancer cell lines (HepG2, Huh7 and SW480) and the control (CCD-18Co). Finally, the line on which the hydrolysate had the greatest effect (SW480) and the control (CCD-18Co) were subjected to the ApoTox-Glo Triplex Assay to determine apoptosis, toxicity, and cell viability. The result showed that hydrolysate had a dose-dependent cytotoxic effect selective on the three cancer cell lines, compared to the control cells. There is a relationship between the antioxidant capacity of RTVHs and their antiproliferative capacity on cancer cells evaluated, which achieved cell viability by action of RTVH of 34.68 and 41.58 and 25.41 %, to HepG2, Huh7 and SW480, respectively. The action of RTVH on cancer cell line SW480 is not due to the induction of apoptosis but to the rupture of the cell membrane.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"42 ","pages":"Article e00832"},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000055/pdfft?md5=68602c527e64d11993eb1f6f609092e3&pid=1-s2.0-S2215017X24000055-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140052660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and modeling of an integrated fixed-film activated sludge (IFAS) system for simultaneous nitrogen and carbon removal from an industrial estate wastewater","authors":"Mina Dolatshah ,&nbsp;Azar Asadi ,&nbsp;Foad Gholami ,&nbsp;Safoora Nazari","doi":"10.1016/j.btre.2024.e00831","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00831","url":null,"abstract":"<div><p>The potential of an integrated fixed film activated sludge (IFAS) bioreactor for developing simultaneous aerobic and anoxic micro-zones under continuous aeration regime to promote carbon and nitrogen removal from Faraman industrial estate wastewater was evaluated in the present research. The effects of three independent variables on carbon and nitrogen removal were assessed. Overall, the optimum condition with 94 %, 77 %, and 2 NTU of COD (chemical oxygen demand) removal, Total nitrogen (TN) removal, and effluent turbidity has been specified with hydraulic retention time (HRT) of 11 h, air flow rate (AFR) of 3.5 L/min, and filling ratio (FR) of 50 %. To assess the stability of treating processes in the system, the IFAS system was operated in this optimal condition. Moreover, the simulation of the bioreactor was accomplished via calibration and verification of GPS-X model. GPSX simulation results and experimental data were compared using an independent sample T-test, which the T-test result confirmed that there was no significant difference between them.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"41 ","pages":"Article e00831"},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000043/pdfft?md5=bed09f616d5e788f3d63f180554bb1ec&pid=1-s2.0-S2215017X24000043-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139732859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}