Biotechnology Reports最新文献

{"title":"Eco-smart biocontrol strategies utilizing potent microbes for sustainable management of phytopathogenic diseases","authors":"Ihtisham Ul Haq ,&nbsp;Kashif Rahim ,&nbsp;Galal Yahya ,&nbsp;Bushra Ijaz ,&nbsp;Sajida Maryam ,&nbsp;Najeeba Parre Paker","doi":"10.1016/j.btre.2024.e00859","DOIUrl":"10.1016/j.btre.2024.e00859","url":null,"abstract":"<div><p>Plants have an impact on the economy because they are used in the food and medical industries. Plants are a source of macro- and micronutrients for the health of humans and animals; however, the rise in microbial diseases has put plant health and yield at risk. Because there are insufficient controls, microbial infections annually impact approximately 25 % of the world's plant crops. Alternative strategies, such as biocontrol, are required to fight these illnesses. This review discusses the potential uses of recently discovered microorganisms because they are safe, effective, and unlikely to cause drug resistance. They have no negative effects on soil microbiology or the environment because they are environmentally benign. Biological control enhances indigenous microbiomes by reducing bacterial wilt, brown blotch, fire blight, and crown gall. More research is required to make these biocontrol agents more stable, effective, and less toxic before they can be used in commercial settings.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"44 ","pages":"Article e00859"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000328/pdfft?md5=021705d5b01889500b27112c5cea23a3&pid=1-s2.0-S2215017X24000328-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically surface-modified Escherichia coli outer membrane vesicles targeting MUC1 antigen in cancer cells","authors":"Sedthawut Laotee ,&nbsp;Wanatchaporn Arunmanee","doi":"10.1016/j.btre.2024.e00854","DOIUrl":"10.1016/j.btre.2024.e00854","url":null,"abstract":"<div><p>Outer membrane vesicles (OMVs), non-replicating spherical liposomes derived from Gram-negative bacteria, are a promising vaccine platform and multifunctional delivery systems. Their ability to be modified via genetic engineering for the incorporation and display of heterologous proteins enhances their functionality. In this study, we demonstrated a bio-ligation approach to display single-chain variable fragments (scFv) on the OMV surface using the SpyTag/SpyCatcher system. SpyTag-fused scFv, expressed by mammalian cells, bound to OMVs with SpyCatcher-fused Lpp'OmpA after a simple incubation. Biophysical analysis indicated that the conjugated OMVs maintained their physicochemical properties. We used an scFv targeting mucin 1 protein (MUC1) for specific cell targeting. Confocal microscopy revealed that conjugated OMVs specifically bound to and were internalized by MUC1-presenting cells, but not by MUC1-deficient cells. In conclusion, this rapid and efficient bio-ligation system facilitates the display of functional scFv on OMV surfaces, offering a promising approach for targeted delivery to MUC1-expressing cancer cells.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"44 ","pages":"Article e00854"},"PeriodicalIF":0.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000274/pdfft?md5=f5efc7af290ccba0cbc5213a769d0654&pid=1-s2.0-S2215017X24000274-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum regarding missing Declaration of Competing Interest statements in previously published article","authors":"","doi":"10.1016/j.btre.2024.e00850","DOIUrl":"10.1016/j.btre.2024.e00850","url":null,"abstract":"","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"43 ","pages":"Article e00850"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000237/pdfft?md5=3494a9bffb4bded9762c4b2f82bf90f9&pid=1-s2.0-S2215017X24000237-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142128215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antarctic Streptomyces: Promising biocontrol agents for combating Fusarium oxysporum f. sp. cubense","authors":"Jeffrey Vargas Perez ,&nbsp;Lizette Serrano ,&nbsp;Rafael Viteri ,&nbsp;Daynet Sosa ,&nbsp;Christian A. Romero ,&nbsp;Nardy Diez","doi":"10.1016/j.btre.2024.e00852","DOIUrl":"10.1016/j.btre.2024.e00852","url":null,"abstract":"<div><p>Fusarium wilt of Banana (FWB) caused by <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> (Foc) poses a significant threat to the banana industry, with current inadequate control measures. This study evaluated the antifungal potential of nine <em>Streptomyces</em> strains isolated from Antarctic soil samples, using Casein-Starch media to stimulate the production of antifungal compounds. The inhibition spectrum against Foc was assessed under laboratory conditions using the well diffusion on Mueller-Hinton agar, with antifungal activity measured in arbitrary units (AU/mL) and minimum inhibitory concentration (MIC) tested using ethyl acetate extracts. Among the nine isolates, K6 and E7 were closely related to <em>Streptomyces polyrhachis</em> and <em>Streptomyces fildesensis</em>, exhibited significant antifungal activity, with K6 and E7 showing 320 and 80 AU/mL, and MIC values of 250 and &gt;500 ppm, respectively. These findings highlight K6 and E7 as potential biocontrol agents against Foc, offering new avenues for sustainable Fusarium wilt management in banana cultivation.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"43 ","pages":"Article e00852"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000250/pdfft?md5=1b44434fa84a60443a329e1137530153&pid=1-s2.0-S2215017X24000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing sustainability in the production of palm oil: creative monitoring methods using YOLOv7 and YOLOv8 for effective plantation management","authors":"Istiyak Mudassir Shaikh ,&nbsp;Mohammad Nishat Akhtar ,&nbsp;Abdul Aabid ,&nbsp;Omar Shabbir Ahmed","doi":"10.1016/j.btre.2024.e00853","DOIUrl":"10.1016/j.btre.2024.e00853","url":null,"abstract":"<div><p>The You Only Look Once (YOLO) deep learning model iterations—YOLOv7–YOLOv8—were put through a rigorous evaluation process to see how well they could recognize oil palm plants. Precision, recall, F1-score, and detection time metrics are analyzed for a variety of configurations, including YOLOv7x, YOLOv7-W6, YOLOv7-D6, YOLOv8s, YOLOv8n, YOLOv8m, YOLOv8l, and YOLOv8x. YOLO label v1.2.1 was used to label a dataset of 80,486 images for training, and 482 drone-captured images, including 5,233 images of oil palms, were used for testing the models. The YOLOv8 series showed notable advancements; with 99.31 %, YOLOv8m obtained the greatest F1-score, signifying the highest detection accuracy. Furthermore, YOLOv8s showed a notable decrease in detection times, improving its suitability for comprehensive environmental surveys and in-the-moment monitoring. Precise identification of oil palm trees is beneficial for improved resource management and less environmental effect; this supports the use of these models in conjunction with drone and satellite imaging technologies for agricultural economic sustainability and optimal crop management.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"44 ","pages":"Article e00853"},"PeriodicalIF":0.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000262/pdfft?md5=4fc6799c82ae9c86555710c14c13266b&pid=1-s2.0-S2215017X24000262-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142130066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fusion protein designed for soluble expression, rapid purification, and enhanced stability of parasporin-2 with potential therapeutic applications","authors":"Monrudee Srisaisap ,&nbsp;Thanya Suwankhajit ,&nbsp;Panadda Boonserm","doi":"10.1016/j.btre.2024.e00851","DOIUrl":"10.1016/j.btre.2024.e00851","url":null,"abstract":"<div><p><em>Bacillus thuringiensis</em> parasporin-2 (PS2Aa1 or Mpp46Aa1) selectively destroys human cancer cells, making it a promising anticancer agent. PS2Aa1 protoxin expression in <em>Escherichia coli</em> typically results in inclusion bodies that must be solubilized and digested by proteinase K to become active. Here, maltose-binding protein (MBP) was fused to the N-terminus of PS2Aa1, either full-length (MBP-fPS2) or truncated (MBP-tPS2), to increase soluble protein expression in <em>E. coli</em> and avoid solubilization and proteolytic activation. Soluble MBP-fPS2 and MBD-tPS2 proteins were produced in <em>E. coli</em> and purified with endotoxin levels below 1 EU/μg. MBP-fPS2 was cytotoxic against T cell leukemia MOLT-4 and Jurkat cell lines after proteinase-K digestion. However, MBP-tPS2 was cytotoxic immediately without MBP tag removal or activation. MBP-tPS2′s thermal stability also makes it appropriate for bioproduction and therapeutic applications.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"43 ","pages":"Article e00851"},"PeriodicalIF":0.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000249/pdfft?md5=0a5deb261e169fd6c41fb525c3a27dd7&pid=1-s2.0-S2215017X24000249-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pressure-driven membrane processes for the recovery and recycling of deep eutectic solvents: A seaweed biorefinery case study.","authors":"Oscar M. Elizondo Sada ,&nbsp;Isa S.A. Hiemstra ,&nbsp;Nattawan Chorhirankul ,&nbsp;Michel Eppink ,&nbsp;Rene H. Wijffels ,&nbsp;Anja E.M. Janssen ,&nbsp;Antoinette Kazbar","doi":"10.1016/j.btre.2024.e00849","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00849","url":null,"abstract":"<div><p>Deep eutectic solvents (DES) are green alternatives for conventional solvents. They have gained attention for their potential to extract valuable compounds from biomass, such as seaweed. In this framework, a case study was developed to assess the feasibility of pressure-driven membrane processes as an efficient tool for the recovery of deep eutectic solvents and targeted biomolecules. For this purpose, a mixture composed of the DES choline chloride – ethylene glycol (ChCl-EG) 1:2, water and alginate was made to mimic a DES extraction from seaweed. An integrated separation process design was proposed where ultrafiltration-diafiltration-nanofiltration (UF-DF-NF) was coupled. UF and DF were found to be effective for the separation of alginate with an 85 % yield. DES was likewise recovered by 93 %, proving the membrane filtrations’ technical feasibility. The NF performance to separate the DES from the water, for its recycling, laid by a 45 %-50 % retention and a final concentrated DES solution of 18 %(v/v).</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"43 ","pages":"Article e00849"},"PeriodicalIF":0.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000225/pdfft?md5=9d8e3de09fb53a8a62f7c382f4637951&pid=1-s2.0-S2215017X24000225-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141540804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of N-glycans on the immune response of plant-produced SARS-CoV-2 RBD-Fc proteins","authors":"Theerakarn Srisangsung ,&nbsp;Thareeya Phetphoung ,&nbsp;Suwimon Manopwisedjaroen ,&nbsp;Kaewta Rattanapisit ,&nbsp;Christine Joy I. Bulaon ,&nbsp;Arunee Thitithanyanont ,&nbsp;Vudhiporn Limprasutr ,&nbsp;Richard Strasser ,&nbsp;Waranyoo Phoolcharoen","doi":"10.1016/j.btre.2024.e00847","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00847","url":null,"abstract":"<div><p>Plant-based manufacturing has the advantage of post-translational modifications. While plant-specific N-glycans have been associated with allergic reactions, their effect on the specific immune response upon vaccination is not yet understood. In this study, we produced an RBD-Fc subunit vaccine in both wildtype (WT) and glycoengineered (∆XF) <em>Nicotiana benthamiana</em> plants. The N-glycan analysis: RBD-Fc carrying the ER retention peptide mainly displayed high mannose. When produced in WT RBD-Fc displayed complex-type (GnGnXF) N-glycans. In contrast, ∆XF plants produced RBD-Fc with humanized complex N-glycans that lack potentially immunogenic xylose and core fucose residues (GnGn). The three recombinant RBD-Fc glycovariants were tested. Immunization with any of the RBD-Fc proteins resulted in a similar titer of anti-RBD antibodies in mice. Likewise, antisera from subunit RBD-Fc vaccines also demonstrated comparable neutralization against SARS-CoV-2. Thus, we conclude that N-glycan modifications of the RBD-Fc protein have no impact on their capacity to activate immune responses and induce neutralizing antibody production.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"43 ","pages":"Article e00847"},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000201/pdfft?md5=2a6a99f12c7f6eaf75696f1d8114a510&pid=1-s2.0-S2215017X24000201-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus sonorinses derived exopolysaccharide enhances cell cycle arrest, apoptosis, necrosis, autophagy and COX-2 down regulation in liver cancer cells","authors":"Salma M. Abdelnasser ,&nbsp;Nourhan Abu-Shahba","doi":"10.1016/j.btre.2024.e00848","DOIUrl":"https://doi.org/10.1016/j.btre.2024.e00848","url":null,"abstract":"<div><h3>Background</h3><p>Hepatocellular carcinoma (HCC) is one of the most serious types of cancer that accounts for numerous cancer deaths worldwide. HCC is poorly prognosed and is a highly chemotherapy-resistant tumor. Therefore, new treatments are urgently needed. Exopolysaccharides (EPS-1) produced from the novel <em>Bacillus sonorensis</em> strain was found to exhibit chemopreventive effects against cancer.</p></div><div><h3>Objective</h3><p>Evaluating the anti-cancer cytotoxic effect of exopolysaccharides (EPS-1) produced by the newly studied <em>Bacillus sonorensis</em> strain SAmt2.</p></div><div><h3>Methods</h3><p>The cytotoxic activity was investigated through cell cycle, apoptosis, and autophagy analyses using flow cytometry technique. Also, the effect of EPS-1 on Huh7 release of COX-2 was examined using ELISA.</p></div><div><h3>Results</h3><p>Our results revealed that EPS-1exhibit an anti-proliferative effect on Huh7 cells through decreasing the percentage of cells at the S-phase and G2 phase, while increasing the cell population at the sub-G1 and G1 phases. Apoptosis analysis showed that EPS-1 increased necrotic and apoptotic cell fractions in EPS-1 treated Huh7. In addition, it induced significant autophagic cell death in the Huh7.Finally, antiproliferative and apoptosis induction results were supportedby ELISA assay results where the protein level of COX-2 was declined.</p></div><div><h3>Conclusion</h3><p>: In conclusion, EPS-1 derived from <em>B. sonorensis</em> SAmt2, is a promising proliferation inhibitor of Huh7 cells with potential anticancer effects.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"43 ","pages":"Article e00848"},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000213/pdfft?md5=3861a0b1ad5638485c2813b661e33e9c&pid=1-s2.0-S2215017X24000213-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesized MgONPs using Syzygium cumini seed extract: Characterization, In vitro anti-oxidant and anti-microbial activity","authors":"Sai Manogna Kotakadi ,&nbsp;Manpreet Jivin Bangarupeta ,&nbsp;Kusuma Kandati ,&nbsp;Deva Prasad Raju Borelli ,&nbsp;Jaheera Anwar Sayyed ,&nbsp;Mannur Ismail Shaik ,&nbsp;John Sushma Nannepaga","doi":"10.1016/j.btre.2024.e00846","DOIUrl":"10.1016/j.btre.2024.e00846","url":null,"abstract":"<div><p>The present study investigates <em>S. cumini</em> seed extracts which are considered as a promising and valuable source of bioactive compounds were prepared using different solvents such as methanol, ethanol, petroleum ether, acetone, chloroform, and diethyl ether. Among these solvents, methanol exhibited the highest extraction with a yield of 42 %. HPLC analysis revealed the highest concentration of quercetin flavonoids (49.62 mg/gm) in the methanolic <em>S. cumini</em> seed extract. Thus, the current work deals with the MgONPs synthesis through a biological approach using different <em>S. cumini</em> seed extracts. <em>In vitro</em> anti-oxidant properties were evaluated, which showed an IC₅₀ value of 22.46 μg/mL for MgONPs synthesized from methanolic extract, surpassing the anti-oxidant potency of ascorbic acid by threefold. By leveraging the rich repository of bioactive compounds found within <em>S. cumini</em> seed extract, this study presents a novel approach to MgONPs synthesis. Exploring the symbiotic relationship between <em>S. cumini</em> seed extract and MgONPs, this research elucidates the pivotal role of bioactive compounds in guiding the formation and properties of nanostructures. Further anti-microbial studies on MgONPs from methanolic <em>S. cumini</em> seed extract were conducted against four different bacterial strains (<em>Escherichia coli, Bacillus subtilis, Staphylococcus aureus,</em> and <em>S. typhimurium</em>), revealing potent anti-microbial activity with 5.3 mm of inhibition for 100 µl against <em>S. typhimurium</em>. These findings suggest that <em>S. cumini</em> is a source of bioactive compounds responsible for the successful synthesis of MgONPs. Characterization studies of MgONPs were also carried out using UV–vis spectroscopy, FTIR, SEM, XRD, DSC and HPLC.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"43 ","pages":"Article e00846"},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215017X24000195/pdfft?md5=a303f380bbbae029242b783d24373898&pid=1-s2.0-S2215017X24000195-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141415298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}