Bacillus sonorinses derived exopolysaccharide enhances cell cycle arrest, apoptosis, necrosis, autophagy and COX-2 down regulation in liver cancer cells

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2024-06-20 DOI:10.1016/j.btre.2024.e00848

Salma M. Abdelnasser , Nourhan Abu-Shahba

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引用次数: 0

Abstract

Background

Hepatocellular carcinoma (HCC) is one of the most serious types of cancer that accounts for numerous cancer deaths worldwide. HCC is poorly prognosed and is a highly chemotherapy-resistant tumor. Therefore, new treatments are urgently needed. Exopolysaccharides (EPS-1) produced from the novel Bacillus sonorensis strain was found to exhibit chemopreventive effects against cancer.

Objective

Evaluating the anti-cancer cytotoxic effect of exopolysaccharides (EPS-1) produced by the newly studied Bacillus sonorensis strain SAmt2.

Methods

The cytotoxic activity was investigated through cell cycle, apoptosis, and autophagy analyses using flow cytometry technique. Also, the effect of EPS-1 on Huh7 release of COX-2 was examined using ELISA.

Results

Our results revealed that EPS-1exhibit an anti-proliferative effect on Huh7 cells through decreasing the percentage of cells at the S-phase and G2 phase, while increasing the cell population at the sub-G1 and G1 phases. Apoptosis analysis showed that EPS-1 increased necrotic and apoptotic cell fractions in EPS-1 treated Huh7. In addition, it induced significant autophagic cell death in the Huh7.Finally, antiproliferative and apoptosis induction results were supportedby ELISA assay results where the protein level of COX-2 was declined.

Conclusion

: In conclusion, EPS-1 derived from B. sonorensis SAmt2, is a promising proliferation inhibitor of Huh7 cells with potential anticancer effects.

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枯草杆菌提取的松香多糖可增强肝癌细胞的细胞周期停滞、凋亡、坏死、自噬和 COX-2 下调功能

背景肝细胞癌（HCC）是最严重的癌症类型之一，造成全球众多癌症患者死亡。肝细胞癌预后不良，是一种对化疗高度耐药的肿瘤。因此，迫切需要新的治疗方法。方法利用流式细胞仪技术，通过细胞周期、细胞凋亡和自噬分析研究了EPS-1的细胞毒活性。结果我们的研究结果表明，EPS-1对Huh7细胞有抗增殖作用，它能降低S期和G2期细胞的比例，同时增加亚G1期和G1期细胞的数量。细胞凋亡分析表明，EPS-1 增加了 EPS-1 处理的 Huh7 细胞中坏死和凋亡细胞的比例。最后，抗增殖和细胞凋亡诱导结果得到了 ELISA 检测结果的支持，其中 COX-2 蛋白水平有所下降：总之，从B. sonorensis SAmt2中提取的EPS-1是一种很有前景的Huh7细胞增殖抑制剂，具有潜在的抗癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.