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Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems 显微镜升级回收:将传统显微镜转变为自动化云集成成像系统
IF 2
HardwareX Pub Date : 2025-03-19 DOI: 10.1016/j.ohx.2025.e00637
Drew Ehrlich , Yohei Rosen , David F. Parks , Kivilcim Doganyigit , Ryan Fenimore , Samira Vera-Choqqueccota , Sebastian Hernandez , Anna Toledo , David Haussler , Sri Kurniawan , Mircea Teodorescu
{"title":"Microscope Upcycling: Transforming legacy microscopes into automated cloud-integrated imaging systems","authors":"Drew Ehrlich ,&nbsp;Yohei Rosen ,&nbsp;David F. Parks ,&nbsp;Kivilcim Doganyigit ,&nbsp;Ryan Fenimore ,&nbsp;Samira Vera-Choqqueccota ,&nbsp;Sebastian Hernandez ,&nbsp;Anna Toledo ,&nbsp;David Haussler ,&nbsp;Sri Kurniawan ,&nbsp;Mircea Teodorescu","doi":"10.1016/j.ohx.2025.e00637","DOIUrl":"10.1016/j.ohx.2025.e00637","url":null,"abstract":"<div><div>Computerized microscopes improve repeatability, throughput, antisepsis, data analysis and data sharing in the biological laboratory, but these machines are cost-prohibitive in most academic environments. This is a barrier into collecting the large and consistent datasets required for machine learning analyses of microscopy data. We demonstrate hardware modifications and software to bring the features of modern computerized microscopes to decades-old legacy laboratory inverted microscopes. We demonstrate automation of X-Y positioning, focus stacking, image acquisition and image storage.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00637"},"PeriodicalIF":2.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design and fabrication of a 3D-printed drone-integrated winching system 3d打印无人机集成绞车系统的设计与制造
IF 2
HardwareX Pub Date : 2025-03-18 DOI: 10.1016/j.ohx.2025.e00642
William Crowe, Benicio Costales, Kyle Luthy
{"title":"Design and fabrication of a 3D-printed drone-integrated winching system","authors":"William Crowe,&nbsp;Benicio Costales,&nbsp;Kyle Luthy","doi":"10.1016/j.ohx.2025.e00642","DOIUrl":"10.1016/j.ohx.2025.e00642","url":null,"abstract":"<div><div>Existing multi-rotor drone-based winching platforms can be expensive, complex, proprietary, and heavy. Here, an inexpensive, simple, effective, and lightweight drone winching platform named the CLARK-3 is described. This winching platform is suitable for payloads of up to 3 kg in its existing configuration and relies heavily on readily available standard parts and 3D printed components while being compatible with a variety of drone controllers. The CLARK-3 in its existing configuration weighs 0.8 kg, can hold up to 27 m of 130 N monofilament, and has a low power consumption at maximum load (∼16 W). Considerations were made during the design of the CLARK-3 to ensure field serviceability by reducing part count and simplifying disassembly.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00642"},"PeriodicalIF":2.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Flexible hardware for teaching and research in renewable energy and off-grid microgrids 用于可再生能源和离网微电网教学和研究的灵活硬件
IF 2
HardwareX Pub Date : 2025-03-18 DOI: 10.1016/j.ohx.2025.e00636
Cristian Escudero-Quintero, Juan Pablo Guzman-Rodriguez, Juan Pablo Villegas-Ceballos, Elkin Edilberto Henao-Bravo, Daniel Gonzalez-Montoya
{"title":"Flexible hardware for teaching and research in renewable energy and off-grid microgrids","authors":"Cristian Escudero-Quintero,&nbsp;Juan Pablo Guzman-Rodriguez,&nbsp;Juan Pablo Villegas-Ceballos,&nbsp;Elkin Edilberto Henao-Bravo,&nbsp;Daniel Gonzalez-Montoya","doi":"10.1016/j.ohx.2025.e00636","DOIUrl":"10.1016/j.ohx.2025.e00636","url":null,"abstract":"<div><div>Microgrids are an active research field due to their integration of renewable and non-renewable energy sources alongside energy storage systems to supply both DC and AC loads. While existing studies extensively explore hierarchical control structures, focusing on algorithm design and optimization, practical implementation remains challenging due to system complexity. This paper presents the development of a low-power microgrid hardware platform based on power converters and controllers, enabling experimental validation of the second and third levels of hierarchical control. The platform enables real-time energy management using FPPT algorithms while providing an intuitive graphical interface for data visualization and system monitoring. Experimental results confirm the system’s capability of dynamically managing energy flows, ensuring stable operation under varying load conditions. The system demonstrates reliable operation handling loads of up to 100 W, with scalability to 200 W, providing an effective tool for testing and research in microgrid control strategies.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00636"},"PeriodicalIF":2.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Open-source portable solar power supply for plasma generators 开源的等离子体发电机便携式太阳能电源
IF 2
HardwareX Pub Date : 2025-03-17 DOI: 10.1016/j.ohx.2025.e00641
Md Motakabbir Rahman , Wei Zhang , Ying Zheng , Joshua M. Pearce
{"title":"Open-source portable solar power supply for plasma generators","authors":"Md Motakabbir Rahman ,&nbsp;Wei Zhang ,&nbsp;Ying Zheng ,&nbsp;Joshua M. Pearce","doi":"10.1016/j.ohx.2025.e00641","DOIUrl":"10.1016/j.ohx.2025.e00641","url":null,"abstract":"<div><div>Non thermal plasmas created by dielectric barrier discharge can break down methane directly to constituent elements without carbon dioxide emissions to produce a high-purity hydrogen and byproduct of solid carbon. To fulfill the environmental promise of plasma generators they must be powered with sustainable energy sources such as solar photovoltaic (PV) systems. There is a need to overcome the limitations of past approaches to power plasma systems to develop a completely open source solar PV system design capable of providing the necessary high-quality power. To overcome this research gap, this article provides a customizable open-source PV-powered design for plasma generators, which allows off-grid operation. This design facilitates the modification of existing lab-grade plasma generator setups into portable solar-powered systems. The open source inverter provides for AC loads at both 120 V and 230 V and has an acceptable total harmonic distortion of 3.58 %. The system was successfully able to power plasma generation and produce high quality plasmas with methane. Plasma generators are highly sensitive to any input voltage variation and power oscillation and the open source system provided higher power applied to the plasma reactor, which resulted in increased CH<sub>4</sub> conversion by 60.5 % and H<sub>2</sub> production 44.7 % compared to grid supply.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00641"},"PeriodicalIF":2.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Autonomous water sampling and quality monitoring in remote locations: A novel approach using a remote-controlled boat 在偏远地区自主取样和水质监测:一种使用遥控船的新方法
IF 2
HardwareX Pub Date : 2025-03-08 DOI: 10.1016/j.ohx.2025.e00634
Ashish Shukla , Robert Ross , Bishakh Bhattacharya , Alex Stumpf
{"title":"Autonomous water sampling and quality monitoring in remote locations: A novel approach using a remote-controlled boat","authors":"Ashish Shukla ,&nbsp;Robert Ross ,&nbsp;Bishakh Bhattacharya ,&nbsp;Alex Stumpf","doi":"10.1016/j.ohx.2025.e00634","DOIUrl":"10.1016/j.ohx.2025.e00634","url":null,"abstract":"<div><div>Water quality varies widely across the globe due to numerous sources of contamination. This disparity emphasizes the urgent need to achieve UN Sustainable Development Goal 6, which aims to ensure universal access to clean water and sanitation. Traditional water monitoring approaches often come with high costs, limited time fidelity, and the absence of territorial dimensionality (often at fixed points). These approaches rely on either manual sampling or stationary buoy platforms, which are labour-intensive and cannot be easily accessed to retrieve water samples (for stationary systems). This paper presents an inexpensive, modified remote-controlled (RC) boat based water monitoring system that is open source, compact, robust, highly adaptable and capable of traversing various riverine environments to collect water and perform samples anywhere within the water body. The solution enhances data quality, facilitates laboratory microbiological investigation, and provides combined water quality data and water samples for comprehensive analysis. The platform comprises a remotely operated boat equipped with lab-grade sensors (pH, dissolved oxygen, conductivity, ORP, temperature) and a sonar depth sensor. It efficiently collects high-resolution spatio-temporal water-quality data with a high accuracy RTK-GPS system and allows eight separate water samples to be collected at different locations. The sensors were validated using lab-grade equipment, followed by successful field testing that confirmed their accuracy and reliability in real-world conditions.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00634"},"PeriodicalIF":2.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles 紧凑的电磁注射器搅拌器和温度控制器,用于可靠地分配活细胞和微粒
IF 2
HardwareX Pub Date : 2025-03-04 DOI: 10.1016/j.ohx.2025.e00638
Maryamsadat Ghoreishi , Giovanna Peruzzi , Lucia Iafrate , Gianluca Cidonio , Noemi D’Abbondanza , Giancarlo Ruocco , Marco Leonetti , Riccardo Reale
{"title":"A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles","authors":"Maryamsadat Ghoreishi ,&nbsp;Giovanna Peruzzi ,&nbsp;Lucia Iafrate ,&nbsp;Gianluca Cidonio ,&nbsp;Noemi D’Abbondanza ,&nbsp;Giancarlo Ruocco ,&nbsp;Marco Leonetti ,&nbsp;Riccardo Reale","doi":"10.1016/j.ohx.2025.e00638","DOIUrl":"10.1016/j.ohx.2025.e00638","url":null,"abstract":"<div><div>Lab-on-chip technologies frequently require biological samples, such as cells or microorganisms, to be maintained inside a syringe for prolonged periods of time during operations. Challenges include preventing cell sedimentation, ensuring cell viability, and maintaining buffer rheological properties (i.e. viscosity and density) constant, particularly in applications like 3D bioprinting and diagnostic assays. To address these challenges, we have developed the Syringe Electromagnetic Controller (SEC), an integrated system capable of simultaneously stirring and thermoregulating samples inside a syringe. SEC prevents sedimentation through the cyclic movement of a magnet actuated by an electromagnetic field, while maintaining a stable temperature (within ± 0.5 °C from a set-point) with a feedback loop. The system is compact, cost-effective, and easily integrated into various setups. Experimental validation shows that SEC effectively keeps living cells in suspension and at a constant temperature without compromising cell viability. Thus, we have ultimately demonstrated the functionality of SEC as a versatile solution for enhancing the reliability of lab-on-chip applications.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00638"},"PeriodicalIF":2.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IoT smartwatch based on open technologies for the collection of thermal comfort data 物联网智能手表基于开放技术采集热舒适数据
IF 2
HardwareX Pub Date : 2025-03-03 DOI: 10.1016/j.ohx.2025.e00633
Julio Landa, Guillermo Barrios, Guadalupe Huelsz
{"title":"IoT smartwatch based on open technologies for the collection of thermal comfort data","authors":"Julio Landa,&nbsp;Guillermo Barrios,&nbsp;Guadalupe Huelsz","doi":"10.1016/j.ohx.2025.e00633","DOIUrl":"10.1016/j.ohx.2025.e00633","url":null,"abstract":"<div><div>This paper presents an IoT smartwatch based on open technologies for thermal comfort data collection. The device performs simplified thermal comfort surveys every hour, collecting data such as clothing insulation level (clo), metabolic activity (met), location, thermal sensation, and thermal acceptance. In addition, it measures two physiological variables: skin temperature and heart rate. The smartwatch is built with low-cost components, including a XIAO ESP32C3 microcontroller, a GY-906 temperature sensor, a MAX30102 heart rate sensor, and Seeed Studio’s XIAO Round Display touchscreen. All collected data are published in real time on an IoT platform that allows remote access to all information. During device registration, the user is prompted to complete a Google Form, where additional data such as gender, age, height, weight, and frequency of air conditioner use are collected. This information, in combination with data from the smartwatch, contributes to a robust database. The ease of use, the design of the device, and its open-source nature make it ideal for the collection of thermal comfort data and its potential use for the generation of adaptative thermal comfort models.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00633"},"PeriodicalIF":2.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low-cost composite autosampler for wastewater sampling 用于废水采样的低成本复合自动进样器
IF 2
HardwareX Pub Date : 2025-03-01 DOI: 10.1016/j.ohx.2025.e00631
Hadi A. Al-agele , Bao D. Nguyen , Liam P. Zimmermann , Gurpreet Singh , Cara Walter , Chet Udell , John S. Selker
{"title":"Low-cost composite autosampler for wastewater sampling","authors":"Hadi A. Al-agele ,&nbsp;Bao D. Nguyen ,&nbsp;Liam P. Zimmermann ,&nbsp;Gurpreet Singh ,&nbsp;Cara Walter ,&nbsp;Chet Udell ,&nbsp;John S. Selker","doi":"10.1016/j.ohx.2025.e00631","DOIUrl":"10.1016/j.ohx.2025.e00631","url":null,"abstract":"<div><div>Wastewater monitoring is a proven method for detecting viruses, illicit drug consumption, pharmaceutical use/abuse, water pollution, and the occurrence of antimicrobial resistance genes. Analyzing sewage water for pathogens like the SARS-Cov-2 virus could enable early detection of infected areas or virus variants. We developed a low-cost (∼US$1000), open-source, composite automatic water sampler with operational flexibility and simple data logging. Sample quantity is measured and cut off appropriately by continuously weighing the incoming liquid. Our system is customizable in both its hardware and firmware (via options and code modification) which allows for the device to be tailored easily to each application. For example, the user can input sampling quantities from 10 g per sample every 1.5 min to a single 2900 g sample after up to a 16-day delay prior to sampling. The outer diameter and height of the sampler are less than 0.5 m to fit in human-entry openings<strong>.</strong> We present experimental data for sample sizes of 10 g and 100 g at suction heights between 0.65 and 4.75 m.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"21 ","pages":"Article e00631"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A portable low-cost polymerase chain reaction device 一种便携式低成本聚合酶链式反应装置
IF 2
HardwareX Pub Date : 2025-03-01 DOI: 10.1016/j.ohx.2025.e00635
Kan Luo , Wei Cheng , Yu Chen , Qirong Zhang , Chaobing Liang , Jianxing Li , Wu Wang
{"title":"A portable low-cost polymerase chain reaction device","authors":"Kan Luo ,&nbsp;Wei Cheng ,&nbsp;Yu Chen ,&nbsp;Qirong Zhang ,&nbsp;Chaobing Liang ,&nbsp;Jianxing Li ,&nbsp;Wu Wang","doi":"10.1016/j.ohx.2025.e00635","DOIUrl":"10.1016/j.ohx.2025.e00635","url":null,"abstract":"<div><div>Polymerase chain reaction (PCR) is a cornerstone technique in molecular biology and clinical diagnostics. However, conventional PCR systems are often bulky and prohibitively expensive, limiting their use in resource-limited settings. In this work, we present a portable, low-cost PCR instrument designed to overcome these challenges while providing fast and accurate thermal cycling. The system features a compact four-well aluminum heating block integrated with a semiconductor thermoelectric cooler and a heated lid, all controlled via an Arduino UNO platform and a piecewise variable coefficient PID algorithm. The device achieves heating and cooling rates of 1.78 °C/s and 1.52 °C/s, respectively, and maintains temperature accuracy within ± 0.55 °C. The power-bank powered prototype measures 210 × 140 × 105 mm<sup>3</sup> and weighs 670 g, making it ideal for resource-constrained applications. Validation experiments, including successful amplification of kelp genes, yielded results comparable to conventional commercial instruments. Open source design files and detailed build instructions are provided under the MIT license, providing a cost-effective and accessible solution for expanding molecular diagnostic capabilities in resource-limited settings.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"21 ","pages":"Article e00635"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Portable pressure chamber for manual camera-assisted monitoring of leaf water potential 用于手动相机辅助监测叶片水势的便携式压力室
IF 2
HardwareX Pub Date : 2025-03-01 DOI: 10.1016/j.ohx.2025.e00632
Willian Viana Campos , José Teixeira Filho , Alcebiades Rebouças São José
{"title":"Portable pressure chamber for manual camera-assisted monitoring of leaf water potential","authors":"Willian Viana Campos ,&nbsp;José Teixeira Filho ,&nbsp;Alcebiades Rebouças São José","doi":"10.1016/j.ohx.2025.e00632","DOIUrl":"10.1016/j.ohx.2025.e00632","url":null,"abstract":"<div><div>This paper presents a detailed description of the assembly and components of a pressure chamber used to measure leaf water potential in leaves, particularly in african mahogany. The assembly and components of this pressure chamber were specifically designed for field experiments. The image capture camera plays a crucial role in recording changes occurring in the sample during measurements. It can also be coupled with image analysis devices to quantify alterations in leaf cells and stomata under varying pressure levels. A cylindrical acrylic support ensures the stability of the camera during the procedure, providing additional protection. The pressure chamber assembly is designed to securely accommodate leaves during measurements, facilitating the manipulation of gas levels and enabling the creation of a controlled atmosphere within the chamber. The sealing valve is an essential component, allowing precise control of internal pressure, preventing leaks, and enabling the study of plant responses under different atmospheric conditions. This pressure chamber proven to be a valuable tool in plant ecophysiology, enabling precise monitoring of plant responses to diverse environmental conditions. Consequently, it contributes significantly to the conservation and sustainable management of african mahogany and other plant species in their natural environments.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"21 ","pages":"Article e00632"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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