HardwareXPub Date : 2024-10-18DOI: 10.1016/j.ohx.2024.e00594
Paulo V.R.M. Silva , Robert B. James , Kathryn L. Russell , Tim D. Fletcher , Maria F.S. Gisi , Oldrich Navratil , Frederic Cherqui , Etienne Cossart
{"title":"An automated low-cost monitoring station for suspended sediments and water level","authors":"Paulo V.R.M. Silva , Robert B. James , Kathryn L. Russell , Tim D. Fletcher , Maria F.S. Gisi , Oldrich Navratil , Frederic Cherqui , Etienne Cossart","doi":"10.1016/j.ohx.2024.e00594","DOIUrl":"10.1016/j.ohx.2024.e00594","url":null,"abstract":"<div><div>The use of low-cost sensors, with open-source code, facilitates greater spatial resolution and flexibility of environmental monitoring, thus generating more information and overcoming limitations of traditional commercial sensors. Measurement of water turbidity using submerged sensors can be problematic in that rapid biofouling requires frequent site visits to remove, clean, calibrate and replace the sensor. We therefore designed an automated system using low-cost commercially-available sensors that pumps water from the stream, samples it for turbidity and purges remaining water, leaving the turbidity sensor dry between measurements, thus greatly reducing the biofouling problem and minimizing operation costs. Our station was able to estimate suspended sediment concentrations between 0 and 6 g/L with a root mean square error (RMSE) around 5 % of the total range, which meets typical research and operational study requirements. The results showed that the monitoring station is capable of monitoring water level and turbidity for long periods without the need of cleaning the turbidity sensor, due to its purge function. We demonstrated that spatially intense measurement of turbidity within catchments and drainage networks can be achieved at a relatively low cost, which allows a better understanding of the main sources of suspended sediments and their spatial and temporal variability.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00594"},"PeriodicalIF":2.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-10-11DOI: 10.1016/j.ohx.2024.e00595
Samuel Olson, McKenna Finley, Raviraj Thakur
{"title":"An open-source, battery-powered, low-cost, and dual-channel pneumatic pulse generator for microfluidic cell-stretch assays","authors":"Samuel Olson, McKenna Finley, Raviraj Thakur","doi":"10.1016/j.ohx.2024.e00595","DOIUrl":"10.1016/j.ohx.2024.e00595","url":null,"abstract":"<div><div>Cells in the body are regularly subjected to mechanical forces that influence their biological fate in terms of morphology, gene expression, and differentiation. The current gold standard method to replicate these effects in vitro is to culture cells on devices with elastic substrates and to impart mechanical stretch using mechanical or pneumatic pull–push methods. Microfluidic device designs offer several advantages in this context for general uniform and controlled stretching. However, the experimental setups are bulky, not user-friendly, and often involve several components that reside outside of the tissue culture incubator. Given the wide utility of mechanical stimulation in in-vitro research, our aim was to create a turn-key research tool that bioengineers can deploy in their cell-stretch assays, without having to deal with the complexity and nuances of ad hoc experimental setups. Here, we present an open-source, battery-powered, dual-channel cyclic pneumatic pulse generator box that can reside within an incubator and is compatible with custom microfluidic cell stretch devices. Our method depends on generating pressure-vacuum pulses simply using a linear miniature pneumatic air cylinder actuated using a continuous servo motor. To the best our knowledge, this is a first example of a completely battery-powered, standalone system that doesn’t have any peripherals residing out of the incubator. We provide a detailed list of different components as well as the step-by-step assembly process. We validate its performance in a cell stretch assay using a commercially available microfluidic chip. Our results show an acute stimulation of cyclic stretching over 8 h on human umbilical vein endothelial cells (HUVECs) resulted in preferential alignment of cells perpendicular to the axis of stretch.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00595"},"PeriodicalIF":2.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142437812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-10-10DOI: 10.1016/j.ohx.2024.e00590
Robert L. Read , Lawrence Kincheloe , Forrest Lee Erickson
{"title":"General Purpose Alarm Device: A programmable annunciator","authors":"Robert L. Read , Lawrence Kincheloe , Forrest Lee Erickson","doi":"10.1016/j.ohx.2024.e00590","DOIUrl":"10.1016/j.ohx.2024.e00590","url":null,"abstract":"<div><div>The General Purpose Alarm Device (GPAD) shines lights and makes loud noises to draw the attention of a human being to a problem. It provides a programmable, 80 character display to provide textual information. As an inexpensive modular annunciator, it is intended to decrease the cost of any system that requires complex monitoring and rapid human intervention. Fundamentally, it is designed to act as a peripheral to a controlling computer or microcontroller. The controller may communicate over a USB (COM) connection or a 5V SPI connection via an RJ12 cable. The GPAD is intended to be as general purpose as possible, so that it can be used to provide alarm functionality for many engineering and scientific projects, hobby machines, instruments, and various situations. The original driving use case is to provide medical alarm capability to the PolyVent open-source mechanical medical ventilator. The GPAD supports 5 alarm levels above “silent” of increasing urgency in terms of light, rhythm, and frequency. It has a mute button. It is based on the Arduino Uno Atmega328 design and is potentially extensible through headers and shields like an Uno. The GPAD includes a printed wiring assembly, firmware for the GPAD peripheral, a simple documented API and a 3D printable enclosure. The repo includes instructions for using a second GPAD as a controller as an example for programming.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00590"},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-10-09DOI: 10.1016/j.ohx.2024.e00592
Christelle Schang , Le Zhang , Baiqian Shi , Monica Nolan , Rachael Poon , David T. McCarthy
{"title":"A low-cost homogenizing device for in-field and remote DNA and RNA extraction","authors":"Christelle Schang , Le Zhang , Baiqian Shi , Monica Nolan , Rachael Poon , David T. McCarthy","doi":"10.1016/j.ohx.2024.e00592","DOIUrl":"10.1016/j.ohx.2024.e00592","url":null,"abstract":"<div><div>Environmental monitoring of microorganisms is critical for the protection and enhancement of human and ecosystem health. Even though these molecular methods have overtaken traditional culture-based methods and become more accessible, these techniques still require expensive equipment and dedicated facilities to process samples which in the context of a global pandemic, remote sampling areas or low-income countries can be extremely challenging. Sample preparation and sample homogenisation are critical steps for molecular-based techniques, especially for the extraction of DNA and RNA. This study developed a low-cost, open-source, freely available 3D printed homogenizer for the processing of DNA and RNA extraction. The BoSL Beater 3D is a portable device that allows researcher to perform bead-beating steps commonly required for environmental sample extraction protocols in the field and without access to main’s power. The BoSL Beater 3D was tested on filtered wastewater samples and passive samplers exposed to wastewater over a 24-hour period and showed similar or better performance to the traditional laboratory bead beater for both the extraction of DNA and RNA. The cost of this 3D homogeniser is roughly $18 AUD ($296 AUD with the jigsaw, which is roughly 57 times cheaper than a traditional bead beater) and has the added usability of being portable and easily adaptable to any type of jigsaw. In combination to newly developed field extraction kits as well as portable PCR machines, this 3D homogeniser could provide the tool necessary to enable access to molecular testing in remote setting as well as developing countries, which may not have access to fully equipped laboratories, but also allow for timely reporting. In addition, the BoSL Beater 3D, in combination with field extraction kit, can allow more flexibility to researchers while sampling, shipping, and processing DNA and RNA samples, whilst maintaining quality of these samples.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00592"},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-10-09DOI: 10.1016/j.ohx.2024.e00593
Vincent Salvadori , Daniel Fäh , Sarina Flühler , Jan Wandeler , Maria J. Jacome , Adrian Koller , Marcel Egli , Simon L. Wuest
{"title":"Portable, low-cost, desktop microscope","authors":"Vincent Salvadori , Daniel Fäh , Sarina Flühler , Jan Wandeler , Maria J. Jacome , Adrian Koller , Marcel Egli , Simon L. Wuest","doi":"10.1016/j.ohx.2024.e00593","DOIUrl":"10.1016/j.ohx.2024.e00593","url":null,"abstract":"<div><div>Light microscopes became essential tools in everyday lab work a long time ago. However, most commercial microscopes are costly, and they are often bulky and heavy. Therefore, microscopes are rarely seen in mobile applications or used by interested amateurs. Here, we present an affordable, portable single-lens microscope. It essentially uses a Raspberry Pi single-board computer, a camera, a touchscreen display, and an LED ring at its core. Apart from brightfield microscopy, contrast-enhancing methods by oblique, dark-field, and Rheinberg illumination are possible, as well. The microscope is ideal for applications that do not require high-end optical components. Due to its low cost and flexible use, it is also suitable for hands-on experiences of the fascinating world not visible by the human eye.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00593"},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-10-08DOI: 10.1016/j.ohx.2024.e00591
Gorkem Anil Al, Nicholas Hedworth, Douglas Tilley, Samer Ahmed, Richmond Afeawo, Uriel Martinez-Hernandez
{"title":"BEATRIX: An open source humanoid head platform for robotics teaching and research","authors":"Gorkem Anil Al, Nicholas Hedworth, Douglas Tilley, Samer Ahmed, Richmond Afeawo, Uriel Martinez-Hernandez","doi":"10.1016/j.ohx.2024.e00591","DOIUrl":"10.1016/j.ohx.2024.e00591","url":null,"abstract":"<div><div>This paper introduces BEATRIX, a novel robotic head designed to bridge the gap between theoretical knowledge and practical experience in the field of robotics at universities. The BEATRIX robot comprises a head actuated by a neck-like mechanism with three stepper motors, two cameras and two microphones for acquisition of visual and audio information from the environment. The robot can be connected to any external computer for the design and implementation of algorithms for applications in human–robot interaction. The proposed robotic platform has been used successfully with undergraduate and master students implementing tasks such as face detection and tracking, sound detection and tracking, robot control and graphical user interfaces. This paper includes lists of all the robot components, assembly instructions, and links to all CAD and software files, facilitating replication and further exploration. The robot design and integration of visual and audio sensors enables the development of engaging educational tutorials and robot experiments, enhancing the teaching and learning experience in robotics.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00591"},"PeriodicalIF":2.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-27DOI: 10.1016/j.ohx.2024.e00587
Santiago Zuñiga , Diego Bravo , Carlos Rengifo
{"title":"Design and construction of a servomechanism using a memory alloy linear actuator","authors":"Santiago Zuñiga , Diego Bravo , Carlos Rengifo","doi":"10.1016/j.ohx.2024.e00587","DOIUrl":"10.1016/j.ohx.2024.e00587","url":null,"abstract":"<div><div>This work shows the design and construction of a servomechanism of a rotator-type joint based on NiTi Shape Memory Alloys (SMA) with an angular position measurement based on a potentiometer sensor and digital electronic position control. The expected application of this prototype is for the use of small charges that emulate the movement of the human being, being bio-inspired and activated by artificial muscles, their potential applications they will be in medical and humanoid robotics. Computer Aided Design (CAD) allows evaluating and validating the most convenient parameters for construction of servomechanism, experimental results validate allowed us to obtain the values of the range of motion <span><math><mrow><mo>±</mo><mspace></mspace><mn>20</mn><mo>°</mo></mrow></math></span> and a maximum torque of 1.01 kg-cm exerted on the axis of rotation for the prototype.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00587"},"PeriodicalIF":2.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-27DOI: 10.1016/j.ohx.2024.e00589
Nelson E. Guevara , Carlos F. Rengifo , Yamir H. Bolaños , Daniel A. Fernández , Wilson A. Sierra , Luis E. Rodríguez
{"title":"Design of a low-cost force insoles to estimate ground reaction forces during human gait","authors":"Nelson E. Guevara , Carlos F. Rengifo , Yamir H. Bolaños , Daniel A. Fernández , Wilson A. Sierra , Luis E. Rodríguez","doi":"10.1016/j.ohx.2024.e00589","DOIUrl":"10.1016/j.ohx.2024.e00589","url":null,"abstract":"<div><div>This paper proposes a low-cost electronic system for estimating ground reaction forces (GRF) during human gait. The device consists of one master node and two slave nodes. The master node sends instructions to slave nodes that sample and store data from two force insoles located at the participant’s feet. These insoles are equipped with 14 piezo-resistive FlexiForce A301 sensors (FSR). The slave nodes are attached to the ankles and feet of each participant. Subsequently, the start command is transmitted through the master node, which is connected to the USB port of a personal computer (PC). Once the walking session is completed, the information obtained by the slave nodes can be downloaded by accessing the access point generated by these devices through Wi-Fi communication. The GRF estimation system was validated with force platforms (<em>BTS Bioengineering P6000, Italy</em>), giving on average a <em>fit</em> measure equal to <span><math><mrow><mn>68</mn><mo>.</mo><mn>71</mn><mtext>%</mtext><mo>±</mo><mn>4</mn><mo>.</mo><mn>80</mn><mtext>%</mtext></mrow></math></span> in dynamic situations. Future versions of this device are expected to increase this <em>fit</em> by using machine learning models.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00589"},"PeriodicalIF":2.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ScarfingTool: An Advanced tool to repair Carbon Fiber Reinforced plastic","authors":"Simone Pio Negri, Massimiliano Nitti, Nicola Mosca, Vito Renò, Ettore Stella","doi":"10.1016/j.ohx.2024.e00588","DOIUrl":"10.1016/j.ohx.2024.e00588","url":null,"abstract":"<div><div>Carbon Fiber Reinforced Plastic (CFRP) is going to assume more and more importance in mechanical and aerospace engineering in the near future. This is due to its intrinsic high lightness and resistance, if compared to traditional metallic materials. CFRP is characterized by a proper production and repair technology. The tool showed in this paper is conceived to repair and verify the restoration of such kind of material. Its application is limited to the first part of the repair process: in jargon the scarfing operation, otherwise called “defect removal”. This operation is similar to a high-speed grinding.</div><div>For this reason, the designed tool can be easily applied to other contexts, such as sanding, polishing or deburring.</div><div>Basically, the ScarfingTool is composed by a high-speed electro-spindle and a 3D smart snapshot sensor. This tool is conceived to be as light as possible to be installed on a light collaborative robot. Moreover, its specific design assures great compliance that, as needed, can be regulated. A high efficiency suction inlet completes the tool and guarantees a clean environment during operation. The scarfing process has already been dealt with in <span><span>[1]</span></span> to which the interested reader is addressed.</div><div>This work is licensed under CC BY-SA 4.0. (<span><span>https://creativecommons.org/licenses/by-sa/4.0</span><svg><path></path></svg></span>)</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00588"},"PeriodicalIF":2.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-19DOI: 10.1016/j.ohx.2024.e00583
Maya Lewis , Eli Silver , Robert Hunt, Daniel M. Harris
{"title":"OpenFlume: An accessible and reproducible benchtop flume for research and education","authors":"Maya Lewis , Eli Silver , Robert Hunt, Daniel M. Harris","doi":"10.1016/j.ohx.2024.e00583","DOIUrl":"10.1016/j.ohx.2024.e00583","url":null,"abstract":"<div><div>Open-channel flumes are an important tool in fluid mechanics research and education. However, the few commercially available small-scale flumes are generally expensive and lack complete characterization. In this work, we present an open-source, low-cost, modular benchtop laboratory flume that is designed to be accessible and reproducible. The flume is assembled from widely available materials and hardware, and fabricated exclusively using tools and machinery commonly found in workshop spaces. The recirculating water flow through the system is driven by a controllable electric coolant pump designed for automobiles. Our design has a flow cross-section of 5 × 5 cm, adjustable flow velocity, and a modest overall footprint. All design files and build instructions are shared in a digital repository ensuring openness and reproducibility. The flow in the test section is characterized using particle image velocimetry (PIV) and is shown to be of high uniformity with low turbulence intensity. Furthermore, direct measurements of the drag force on a submerged sphere are reported for a range of control parameters, and exhibit good agreement with established empirical values.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00583"},"PeriodicalIF":2.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}