HardwareXPub Date : 2024-10-09DOI: 10.1016/j.ohx.2024.e00592
Christelle Schang , Le Zhang , Baiqian Shi , Monica Nolan , Rachael Poon , David T. McCarthy
{"title":"A low-cost homogenizing device for in-field and remote DNA and RNA extraction","authors":"Christelle Schang , Le Zhang , Baiqian Shi , Monica Nolan , Rachael Poon , David T. McCarthy","doi":"10.1016/j.ohx.2024.e00592","DOIUrl":"10.1016/j.ohx.2024.e00592","url":null,"abstract":"<div><div>Environmental monitoring of microorganisms is critical for the protection and enhancement of human and ecosystem health. Even though these molecular methods have overtaken traditional culture-based methods and become more accessible, these techniques still require expensive equipment and dedicated facilities to process samples which in the context of a global pandemic, remote sampling areas or low-income countries can be extremely challenging. Sample preparation and sample homogenisation are critical steps for molecular-based techniques, especially for the extraction of DNA and RNA. This study developed a low-cost, open-source, freely available 3D printed homogenizer for the processing of DNA and RNA extraction. The BoSL Beater 3D is a portable device that allows researcher to perform bead-beating steps commonly required for environmental sample extraction protocols in the field and without access to main’s power. The BoSL Beater 3D was tested on filtered wastewater samples and passive samplers exposed to wastewater over a 24-hour period and showed similar or better performance to the traditional laboratory bead beater for both the extraction of DNA and RNA. The cost of this 3D homogeniser is roughly $18 AUD ($296 AUD with the jigsaw, which is roughly 57 times cheaper than a traditional bead beater) and has the added usability of being portable and easily adaptable to any type of jigsaw. In combination to newly developed field extraction kits as well as portable PCR machines, this 3D homogeniser could provide the tool necessary to enable access to molecular testing in remote setting as well as developing countries, which may not have access to fully equipped laboratories, but also allow for timely reporting. In addition, the BoSL Beater 3D, in combination with field extraction kit, can allow more flexibility to researchers while sampling, shipping, and processing DNA and RNA samples, whilst maintaining quality of these samples.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00592"},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-10-09DOI: 10.1016/j.ohx.2024.e00593
Vincent Salvadori , Daniel Fäh , Sarina Flühler , Jan Wandeler , Maria J. Jacome , Adrian Koller , Marcel Egli , Simon L. Wuest
{"title":"Portable, low-cost, desktop microscope","authors":"Vincent Salvadori , Daniel Fäh , Sarina Flühler , Jan Wandeler , Maria J. Jacome , Adrian Koller , Marcel Egli , Simon L. Wuest","doi":"10.1016/j.ohx.2024.e00593","DOIUrl":"10.1016/j.ohx.2024.e00593","url":null,"abstract":"<div><div>Light microscopes became essential tools in everyday lab work a long time ago. However, most commercial microscopes are costly, and they are often bulky and heavy. Therefore, microscopes are rarely seen in mobile applications or used by interested amateurs. Here, we present an affordable, portable single-lens microscope. It essentially uses a Raspberry Pi single-board computer, a camera, a touchscreen display, and an LED ring at its core. Apart from brightfield microscopy, contrast-enhancing methods by oblique, dark-field, and Rheinberg illumination are possible, as well. The microscope is ideal for applications that do not require high-end optical components. Due to its low cost and flexible use, it is also suitable for hands-on experiences of the fascinating world not visible by the human eye.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00593"},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-10-08DOI: 10.1016/j.ohx.2024.e00591
Gorkem Anil Al, Nicholas Hedworth, Douglas Tilley, Samer Ahmed, Richmond Afeawo, Uriel Martinez-Hernandez
{"title":"BEATRIX: An open source humanoid head platform for robotics teaching and research","authors":"Gorkem Anil Al, Nicholas Hedworth, Douglas Tilley, Samer Ahmed, Richmond Afeawo, Uriel Martinez-Hernandez","doi":"10.1016/j.ohx.2024.e00591","DOIUrl":"10.1016/j.ohx.2024.e00591","url":null,"abstract":"<div><div>This paper introduces BEATRIX, a novel robotic head designed to bridge the gap between theoretical knowledge and practical experience in the field of robotics at universities. The BEATRIX robot comprises a head actuated by a neck-like mechanism with three stepper motors, two cameras and two microphones for acquisition of visual and audio information from the environment. The robot can be connected to any external computer for the design and implementation of algorithms for applications in human–robot interaction. The proposed robotic platform has been used successfully with undergraduate and master students implementing tasks such as face detection and tracking, sound detection and tracking, robot control and graphical user interfaces. This paper includes lists of all the robot components, assembly instructions, and links to all CAD and software files, facilitating replication and further exploration. The robot design and integration of visual and audio sensors enables the development of engaging educational tutorials and robot experiments, enhancing the teaching and learning experience in robotics.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00591"},"PeriodicalIF":2.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-27DOI: 10.1016/j.ohx.2024.e00587
Santiago Zuñiga , Diego Bravo , Carlos Rengifo
{"title":"Design and construction of a servomechanism using a memory alloy linear actuator","authors":"Santiago Zuñiga , Diego Bravo , Carlos Rengifo","doi":"10.1016/j.ohx.2024.e00587","DOIUrl":"10.1016/j.ohx.2024.e00587","url":null,"abstract":"<div><div>This work shows the design and construction of a servomechanism of a rotator-type joint based on NiTi Shape Memory Alloys (SMA) with an angular position measurement based on a potentiometer sensor and digital electronic position control. The expected application of this prototype is for the use of small charges that emulate the movement of the human being, being bio-inspired and activated by artificial muscles, their potential applications they will be in medical and humanoid robotics. Computer Aided Design (CAD) allows evaluating and validating the most convenient parameters for construction of servomechanism, experimental results validate allowed us to obtain the values of the range of motion <span><math><mrow><mo>±</mo><mspace></mspace><mn>20</mn><mo>°</mo></mrow></math></span> and a maximum torque of 1.01 kg-cm exerted on the axis of rotation for the prototype.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00587"},"PeriodicalIF":2.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-27DOI: 10.1016/j.ohx.2024.e00589
Nelson E. Guevara , Carlos F. Rengifo , Yamir H. Bolaños , Daniel A. Fernández , Wilson A. Sierra , Luis E. Rodríguez
{"title":"Design of a low-cost force insoles to estimate ground reaction forces during human gait","authors":"Nelson E. Guevara , Carlos F. Rengifo , Yamir H. Bolaños , Daniel A. Fernández , Wilson A. Sierra , Luis E. Rodríguez","doi":"10.1016/j.ohx.2024.e00589","DOIUrl":"10.1016/j.ohx.2024.e00589","url":null,"abstract":"<div><div>This paper proposes a low-cost electronic system for estimating ground reaction forces (GRF) during human gait. The device consists of one master node and two slave nodes. The master node sends instructions to slave nodes that sample and store data from two force insoles located at the participant’s feet. These insoles are equipped with 14 piezo-resistive FlexiForce A301 sensors (FSR). The slave nodes are attached to the ankles and feet of each participant. Subsequently, the start command is transmitted through the master node, which is connected to the USB port of a personal computer (PC). Once the walking session is completed, the information obtained by the slave nodes can be downloaded by accessing the access point generated by these devices through Wi-Fi communication. The GRF estimation system was validated with force platforms (<em>BTS Bioengineering P6000, Italy</em>), giving on average a <em>fit</em> measure equal to <span><math><mrow><mn>68</mn><mo>.</mo><mn>71</mn><mtext>%</mtext><mo>±</mo><mn>4</mn><mo>.</mo><mn>80</mn><mtext>%</mtext></mrow></math></span> in dynamic situations. Future versions of this device are expected to increase this <em>fit</em> by using machine learning models.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00589"},"PeriodicalIF":2.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ScarfingTool: An Advanced tool to repair Carbon Fiber Reinforced plastic","authors":"Simone Pio Negri, Massimiliano Nitti, Nicola Mosca, Vito Renò, Ettore Stella","doi":"10.1016/j.ohx.2024.e00588","DOIUrl":"10.1016/j.ohx.2024.e00588","url":null,"abstract":"<div><div>Carbon Fiber Reinforced Plastic (CFRP) is going to assume more and more importance in mechanical and aerospace engineering in the near future. This is due to its intrinsic high lightness and resistance, if compared to traditional metallic materials. CFRP is characterized by a proper production and repair technology. The tool showed in this paper is conceived to repair and verify the restoration of such kind of material. Its application is limited to the first part of the repair process: in jargon the scarfing operation, otherwise called “defect removal”. This operation is similar to a high-speed grinding.</div><div>For this reason, the designed tool can be easily applied to other contexts, such as sanding, polishing or deburring.</div><div>Basically, the ScarfingTool is composed by a high-speed electro-spindle and a 3D smart snapshot sensor. This tool is conceived to be as light as possible to be installed on a light collaborative robot. Moreover, its specific design assures great compliance that, as needed, can be regulated. A high efficiency suction inlet completes the tool and guarantees a clean environment during operation. The scarfing process has already been dealt with in <span><span>[1]</span></span> to which the interested reader is addressed.</div><div>This work is licensed under CC BY-SA 4.0. (<span><span>https://creativecommons.org/licenses/by-sa/4.0</span><svg><path></path></svg></span>)</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00588"},"PeriodicalIF":2.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-19DOI: 10.1016/j.ohx.2024.e00583
Maya Lewis , Eli Silver , Robert Hunt, Daniel M. Harris
{"title":"OpenFlume: An accessible and reproducible benchtop flume for research and education","authors":"Maya Lewis , Eli Silver , Robert Hunt, Daniel M. Harris","doi":"10.1016/j.ohx.2024.e00583","DOIUrl":"10.1016/j.ohx.2024.e00583","url":null,"abstract":"<div><div>Open-channel flumes are an important tool in fluid mechanics research and education. However, the few commercially available small-scale flumes are generally expensive and lack complete characterization. In this work, we present an open-source, low-cost, modular benchtop laboratory flume that is designed to be accessible and reproducible. The flume is assembled from widely available materials and hardware, and fabricated exclusively using tools and machinery commonly found in workshop spaces. The recirculating water flow through the system is driven by a controllable electric coolant pump designed for automobiles. Our design has a flow cross-section of 5 × 5 cm, adjustable flow velocity, and a modest overall footprint. All design files and build instructions are shared in a digital repository ensuring openness and reproducibility. The flow in the test section is characterized using particle image velocimetry (PIV) and is shown to be of high uniformity with low turbulence intensity. Furthermore, direct measurements of the drag force on a submerged sphere are reported for a range of control parameters, and exhibit good agreement with established empirical values.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00583"},"PeriodicalIF":2.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-13DOI: 10.1016/j.ohx.2024.e00585
Jeremiah Takyi, Heather R. Beem
{"title":"Design & automation of a small-scale towing tank for flow visualization","authors":"Jeremiah Takyi, Heather R. Beem","doi":"10.1016/j.ohx.2024.e00585","DOIUrl":"10.1016/j.ohx.2024.e00585","url":null,"abstract":"<div><p>Although the towing tank is a standard piece of equipment used to investigate fluid phenomena, it primarily exists as custom-built hardware that takes up a significant footprint. The size, cost, and custom-built nature have heretofore inhibited the production of this equipment in the authors’ context, an African university. This paper presents a small-scale (1000 mm x 200 mm x 200 mm), low-cost (<$1,000) towing tank made using readily available components and basic digital fabrication tools. Other universities on the continent and beyond can hence create this foundational platform for fluid mechanics-related teaching and research. Leveraging an Arduino microcontroller loaded with the GRBL firmware, G-code is sent from the computer to stepper motors to execute movements in two axes. This allows for automation capabilities, controlled towing speeds, and consistent experimental conditions. Validation tests revealed motion accuracy within 1 %. A glitter-based flow visualization approach to measuring surface phenomena is demonstrated here. Experiments conducted successfully visualized relevant flow characteristics generated by bluff bodies being towed in the tank. As the Reynolds number increased within the operating range, wider wakes and larger, more distinct vortices were generated, as expected. This platform can be replicated widely in institutions that may otherwise forego experimentation in fluid mechanics.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00585"},"PeriodicalIF":2.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000798/pdfft?md5=33a5afc6aa1702b24f0e866d4e6d7d8b&pid=1-s2.0-S2468067224000798-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142173243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-11DOI: 10.1016/j.ohx.2024.e00582
Gustavo N. Santiago , Ignacio Ciampitti
{"title":"Multiple channels, low-cost, and dual data storage data logger for building a soil temperature network","authors":"Gustavo N. Santiago , Ignacio Ciampitti","doi":"10.1016/j.ohx.2024.e00582","DOIUrl":"10.1016/j.ohx.2024.e00582","url":null,"abstract":"<div><p>Temperature measurement is critical in many areas of research, particularly in agriculture, where it can have a significant impact on crop health and yield. Experiments such as seed germination often require numerous temperature sensors to collect extensive data. Typically, data loggers are used to store information, but market options are expensive and offer limited, non-customizable inputs (channels), creating challenges for comprehensive soil temperature monitoring. This study aims to develop a network of open-source, low-cost data loggers with multiple customizable channels for local and remote temperature data storage. The hardware includes Arduino, temperature sensors, a Real Time Clock, and a LoRa module to transmit data to a LILYGO TTGO board, which sends it to a remote MongoDB database while also storing it locally on a microSD card. In addition, a digital tool was developed to retrieve and display both current and historical readings from the MongoDB database. The total cost of this hardware is approximately US$ 72 (based on current prices) for the simplest network, which is approximately 18 % of the commercial cost. The system achieved a root mean square error (RMSE) of 1.6 °C compared to a manual sampling probe thermometer, proving it to be a reliable measurement source. The hardware developed in this study surpasses commercial options by allowing the integration of multiple sensors and emitters, creating a network of data loggers at a lower cost. In addition to the hardware, an open-source digital tool was developed to visualize historical data at no additional cost.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00582"},"PeriodicalIF":2.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000762/pdfft?md5=776090d0e6183fb5d682a02ca49aa7c1&pid=1-s2.0-S2468067224000762-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2024-09-10DOI: 10.1016/j.ohx.2024.e00584
Andrea Contina , Eric Abelson , Brendan Allison , Brian Stokes , Kenedy F. Sanchez , Henry M. Hernandez , Anna M. Kepple , Quynhmai Tran , Isabella Kazen , Katherine A. Brown , Je’aime H. Powell , Timothy H. Keitt
{"title":"BioSense: An automated sensing node for organismal and environmental biology","authors":"Andrea Contina , Eric Abelson , Brendan Allison , Brian Stokes , Kenedy F. Sanchez , Henry M. Hernandez , Anna M. Kepple , Quynhmai Tran , Isabella Kazen , Katherine A. Brown , Je’aime H. Powell , Timothy H. Keitt","doi":"10.1016/j.ohx.2024.e00584","DOIUrl":"10.1016/j.ohx.2024.e00584","url":null,"abstract":"<div><p>Automated remote sensing has revolutionized the fields of wildlife ecology and environmental science. Yet, a cost-effective and flexible approach for large scale monitoring has not been fully developed, resulting in a limited collection of high-resolution data. Here, we describe BioSense, a low-cost and fully programmable automated sensing platform for applications in bioacoustics and environmental studies. Our design offers customization and flexibility to address a broad array of research goals and field conditions. Each BioSense is programmed through an integrated Raspberry Pi computer board and designed to collect and analyze avian vocalizations while simultaneously collecting temperature, humidity, and soil moisture data. We illustrate the different steps involved in manufacturing this sensor including hardware and software design and present the results of our laboratory and field testing in southwestern United States.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"20 ","pages":"Article e00584"},"PeriodicalIF":2.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000786/pdfft?md5=ad6045c212fa76ca2cac78c8944e97b3&pid=1-s2.0-S2468067224000786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}