HardwareX最新文献_第10页

Pi-seq—A customizable multichannel syringe pump for microfluidics Pi-seq-A 用于微流控的可定制多通道注射泵

IF 2.2

HardwareX Pub Date : 2024-03-05 DOI: 10.1016/j.ohx.2024.e00517

Tim L. Czech , Philipp P. Nelson , Clemens Thölken , Patrick Meyer , Timo Hess , Ho-Ryun Chung , Till Adhikary

{"title":"Pi-seq—A customizable multichannel syringe pump for microfluidics","authors":"Tim L. Czech , Philipp P. Nelson , Clemens Thölken , Patrick Meyer , Timo Hess , Ho-Ryun Chung , Till Adhikary","doi":"10.1016/j.ohx.2024.e00517","DOIUrl":"https://doi.org/10.1016/j.ohx.2024.e00517","url":null,"abstract":"<div><p>The advent of single cell technologies resulted in growing demand for microfluidics in the biological sciences. Commercial platforms have remained expensive, inflexible, and non-customizable black boxes. We developed an open source, multichannel, zero-backflow microfluidics device based on syringe pumps controlled by a Raspberry Pi computer. It uses both readily available and 3D-printed parts as well as a custom PCB and is easily serviceable. Moreover, it is fully customizable for various applications. Total cost is under €600. We equipped one channel with a custom Peltier-based temperature controller for precise heating or cooling and a mixer mechanism to prevent sedimentation of the cells within the syringe. Depending on the cells in the sample, heating and cooling can be useful to maintain a beneficial environment or to slow down cellular processes and cell death, respectively. Combined with microfluidics consumables and a microscope, the device is capable of integration into a high quality droplet-based single cell RNA sequencing workflow as shown here. Analysis of a mixture of human and insect cells resulted in a dataset of 17,769 single cells and demonstrates reliable operation and separation.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"18 ","pages":"Article e00517"},"PeriodicalIF":2.2,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000117/pdfft?md5=2b420c34a8516c9b5fc0bb0d5cd0d048&pid=1-s2.0-S2468067224000117-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140052390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BioCloneBot: A versatile, low-cost, and open-source automated liquid handler BioCloneBot：多功能、低成本、开源自动液体处理机

IF 2.2

HardwareX Pub Date : 2024-02-28 DOI: 10.1016/j.ohx.2024.e00516

Ke’Koa CDH Wells , Nawwaf Kharma , Brandon B. Jaunky , Kaiyu Nie , Gabriel Aguiar-Tawil , Daniel Berry

{"title":"BioCloneBot: A versatile, low-cost, and open-source automated liquid handler","authors":"Ke’Koa CDH Wells , Nawwaf Kharma , Brandon B. Jaunky , Kaiyu Nie , Gabriel Aguiar-Tawil , Daniel Berry","doi":"10.1016/j.ohx.2024.e00516","DOIUrl":"https://doi.org/10.1016/j.ohx.2024.e00516","url":null,"abstract":"<div><p>Liquid handler systems can provide significant benefits to researchers by automating laboratory work, however, their unaffordable price provides a steep barrier to entry. Therefore, we provide the BioCloneBot, a versatile, low-cost, and open-source automated liquid handler. This system can be easily built with 3D-printed parts and readily available commercial components. The BioCloneBot is highly adaptive to user needs and facilitates various liquid handling tasks in research and diagnostics. Its user-friendly interface and programmable nature make it suitable for a wide range of applications, from small-scale experiments to larger laboratory setups. By utilizing BioCloneBot, researchers and scientists can streamline their liquid handling processes without the financial constraints posed by traditional systems. In this paper, we detail the design, construction, and validation of BioCloneBot, showcasing its precise control, accuracy, and repeatability in various liquid handling tasks. The open-source nature of the system encourages collaboration and customization, enabling researchers to contribute and adapt the technology to specific experimental requirements.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"18 ","pages":"Article e00516"},"PeriodicalIF":2.2,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000105/pdfft?md5=a0cb106f75004b6841e4acba7046051d&pid=1-s2.0-S2468067224000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140122478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual feed progressive cavity pump extrusion system for functionally graded direct ink write 3D printing 用于功能分级直接写墨三维打印的双进料螺杆泵挤出系统

IF 2.2

HardwareX Pub Date : 2024-02-12 DOI: 10.1016/j.ohx.2024.e00515

Max J. Sevcik , Jacob Golson , Gabriel Bjerke , Isaac Snyder , Gage Taylor , Finnegan Wilson , Grace I. Rabinowitz , Dylan J. Kline , Michael D. Grapes , Kyle T. Sullivan , Jonathan L. Belof , Veronica Eliasson

{"title":"Dual feed progressive cavity pump extrusion system for functionally graded direct ink write 3D printing","authors":"Max J. Sevcik , Jacob Golson , Gabriel Bjerke , Isaac Snyder , Gage Taylor , Finnegan Wilson , Grace I. Rabinowitz , Dylan J. Kline , Michael D. Grapes , Kyle T. Sullivan , Jonathan L. Belof , Veronica Eliasson","doi":"10.1016/j.ohx.2024.e00515","DOIUrl":"https://doi.org/10.1016/j.ohx.2024.e00515","url":null,"abstract":"<div><p>Material extrusion Additive Manufacturing (AM), is one of the most widely practiced methods of AM. Fused Filament Fabrication (FFF) is what most associate with AM, as it is relatively inexpensive, and highly accessible, involving feeding plastic filament into a hot-end that melts and extrudes from a nozzle as the toolhead moves along the toolpath. Direct Ink Write (DIW) 3D printing falls into this same category of AM, however is primarily practiced in laboratory settings to construct novel parts from flowable feedstock materials. DIW printers are relatively expensive and often depend on custom software to print a part, limiting user-specificity. There have been recent advancements in multi-material and functionally graded DIW, but the systems are highly custom and the methods used to achieve multi-material prints are openly available to the public. The following article outlines the construction and operation method of a DIW system that is capable of printing that can produce compositionally-graded components using a dual feed progressive cavity pump extruder equipped with a dynamic mixer. The extruder and its capabilities to vary material composition while printing are demonstrated using a Prusa i3 MK3S+ desktop fused filament fabrication printer as the gantry system. This provides users ease of operation, and the capability of further tailoring to specific needs.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"17 ","pages":"Article e00515"},"PeriodicalIF":2.2,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000099/pdfft?md5=6f6ecfe5903d28977ac54c27dc769440&pid=1-s2.0-S2468067224000099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139738457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “An open-source, automated, gas sampling peripheral for laboratory incubation experiments using cavity ring-down spectroscopy” [HardwareX 10 (2021) e00208] 使用空腔环降光谱进行实验室培养实验的开放源码自动气体采样外围设备 "的更正

IF 2.2

HardwareX Pub Date : 2024-02-09 DOI: 10.1016/j.ohx.2024.e00514

Timothy D. Berry , Chance Creelman , Nick Nickerson , Akio Enders , Thea Whitman

引用次数: 0

Developing a remote gamma-ray spectra collection system (RGSCS) by coupling a high purity Germanium (HPGe) detector with a cosmicguard background reduction device 通过将高纯锗（HPGe）探测器与宇宙卫士背景消减装置相结合，开发远程伽马射线光谱收集系统（RGSCS）

IF 2.2

HardwareX Pub Date : 2024-02-02 DOI: 10.1016/j.ohx.2024.e00513

Zaijing Sun, Krishnakumar Divakar Nangeelil, Haven Searcy

{"title":"Developing a remote gamma-ray spectra collection system (RGSCS) by coupling a high purity Germanium (HPGe) detector with a cosmicguard background reduction device","authors":"Zaijing Sun, Krishnakumar Divakar Nangeelil, Haven Searcy","doi":"10.1016/j.ohx.2024.e00513","DOIUrl":"https://doi.org/10.1016/j.ohx.2024.e00513","url":null,"abstract":"<div><p>Despite being widely used for high-resolution spectral analysis and quantifying low activity in natural samples, the operations and data analysis of High Purity Germanium (HPGe) gamma-ray detectors are seldom fully automated due to the excessive costs associated with commercially available automatic sample changing systems. This paper introduces the design and implementation of a cost-effective, customized remote gamma-ray spectra collection system centered around the HPGe detector coupled to a cosmic-ray veto background reduction device. The HPGe detector system, equipped with a Lynx DSA, is seamlessly integrated with an economically viable automatic sample changer. This sample vial changer is controlled by a high-torque NEMA 34 stepper servo motor from Vention. Web control of the rotary actuator is facilitated through a CAD-based programming tool. The remote-controlled sample pick-and-place procedure is executed using a robotic arm (Trossen Robotics, Viper X 250). The DYNAMIXEL servomotors of the robotic arm are programmed using Python software supported by the Robotic Operating System. Beyond its technical construction, this system is uniquely fashioned for academic research, providing invaluable hands-on experience in gamma spectrometry to both junior researchers and students.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"17 ","pages":"Article e00513"},"PeriodicalIF":2.2,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000075/pdfft?md5=61b3d74d0f4d9327a85dbbddb40590ea&pid=1-s2.0-S2468067224000075-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139682456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obstructive respiratory disease simulation device 阻塞性呼吸道疾病模拟装置

IF 2.2

HardwareX Pub Date : 2024-02-01 DOI: 10.1016/j.ohx.2024.e00512

Jaimey A. Clifton, Ella F.S. Guy, Jennifer L. Knopp, J. Geoffrey Chase

{"title":"Obstructive respiratory disease simulation device","authors":"Jaimey A. Clifton, Ella F.S. Guy, Jennifer L. Knopp, J. Geoffrey Chase","doi":"10.1016/j.ohx.2024.e00512","DOIUrl":"https://doi.org/10.1016/j.ohx.2024.e00512","url":null,"abstract":"<div><p>Respiratory disease is a major contributor to healthcare costs, as well as increasing morbidity and early mortality. The device presented is used to simulate the effects of Chronic Obstructive Pulmonary Disease (COPD) in healthy people. The intended use is to provide data equivalent to COPD data measured from those who are ill for initial validation of respiratory mechanics models. It would thus eliminate the need to test unhealthy and/or fragile subjects, or the need for invasive or costly equipment based test methods. The device is used in conjunction with an open-access venturi-based flow sensor, to measure pressure, flow, and breath tidal volume. The device simulates the pressure and flow profiles of a person who has COPD including the non-linear increased resistance to end-exhalation and gas trapping. To achieve this non-linearity, a combination of high and low resistance outlets is used. Thus, the simulator allows the collection of patient-specific COPD-like breathing data in a non-invasive manner from healthy subjects. The device is low-cost with the majority of the parts 3D printed using a Prusa mini 3D printer and PLA filament.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"17 ","pages":"Article e00512"},"PeriodicalIF":2.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000063/pdfft?md5=7f39e36fbd4618c176a5566ad3f50c8e&pid=1-s2.0-S2468067224000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139682435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P-TDHM: Open-source portable telecentric digital holographic microscope P-TDHM：开源便携式远心数字全息显微镜

IF 2.2

HardwareX Pub Date : 2024-01-26 DOI: 10.1016/j.ohx.2024.e00508

Lei Jin , Ziyang Yu , Aaron Au , Peter Serles , Nan Wang , Jeremy T. Lant , Tobin Filleter , Christopher M. Yip

引用次数: 0

HYKYHT: A versatile, affordable, and open-source 3D-printed liquid aspiration system HYKYHT：多功能、经济实惠且开源的 3D 打印液体抽吸系统

IF 2.2

HardwareX Pub Date : 2024-01-24 DOI: 10.1016/j.ohx.2024.e00509

Teng-Cheong Ha , Michael Morgan , Axel Schambach

引用次数: 0

The Enderstruder: An accessible open-source syringe extruder compatible with Ender series 3D printers Enderstruder：与 Ender 系列三维打印机兼容的可访问开源注射器挤出机

IF 2.2

HardwareX Pub Date : 2024-01-24 DOI: 10.1016/j.ohx.2024.e00510

Domenic J. Cordova , Angel A. Rodriguez , Sabrina C. Woodward , Cody O. Crosby

{"title":"The Enderstruder: An accessible open-source syringe extruder compatible with Ender series 3D printers","authors":"Domenic J. Cordova , Angel A. Rodriguez , Sabrina C. Woodward , Cody O. Crosby","doi":"10.1016/j.ohx.2024.e00510","DOIUrl":"https://doi.org/10.1016/j.ohx.2024.e00510","url":null,"abstract":"<div><p>Bioprinting has enabled the precise spatiotemporal deposition of cells and biomaterials, opening new avenues of research in tissue engineering and regenerative medicine. Although several open-source syringe extruder adaptations for bioprinters have been published and adopted by end users, only one has been specifically adapted for the Ender series, an affordable and open-source line of thermoplastic 3D printers. Here, we introduce the Enderstruder, a cost-effective extruder attachment that uses a standard 10 mL BD syringe, positions the stepper motor at the level of the gantry, enhances x-axis stability with a linear rail, and uses the originally included stepper motor, resulting in reduced cost and simplified assembly. Furthermore, we present an iterative process to fine-tune printing profiles for high-viscosity biomaterial inks. To facilitate the implementation of our work by other researchers, we provide fully editable Cura profiles for five commonly used biomaterials. Using these five materials to validate and characterize our design, we employ the Enderstruder to print established calibration patterns and complex shapes. By presenting the Enderstruder and its iterative development process, this study contributes to the growing repository of open-source bioprinting solutions, fostering greater accessibility and affordability for researchers in tissue engineering.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"17 ","pages":"Article e00510"},"PeriodicalIF":2.2,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S246806722400004X/pdfft?md5=e227e52c8fd2a35dfc8a968c47f3fee0&pid=1-s2.0-S246806722400004X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A low-cost, wireless, 4-channel EEG measurement system used in virtual reality environments 用于虚拟现实环境的低成本无线 4 通道脑电图测量系统

IF 2.2

HardwareX Pub Date : 2024-01-24 DOI: 10.1016/j.ohx.2024.e00507

Zhiyuan Yu , Shengwen Guo

{"title":"A low-cost, wireless, 4-channel EEG measurement system used in virtual reality environments","authors":"Zhiyuan Yu , Shengwen Guo","doi":"10.1016/j.ohx.2024.e00507","DOIUrl":"10.1016/j.ohx.2024.e00507","url":null,"abstract":"<div><p>The combination of Virtual Reality (VR) technology and Electroencephalography (EEG) measurements has shown tremendous potential in the fields of psychology and neuroscience research. However, the majority of EEG measurement devices currently available are expensive, bulky, uncomfortable to wear, and difficult to integrate with VR headsets. These limitations have hindered the development of related research fields. This study describes a low-cost (60.07 USD), small-sized, wireless, high-precision, low-power consumption 4-channel EEG measurement system (NeuroVista) for frontal area EEG measurements, which can be used with a VR headset, enabling EEG measurements in VR environments. The system has an input-referred noise of less than 0.9480 <span><math><mrow><mi>μ</mi><mi>V</mi><mi>r</mi><mi>m</mi><mi>s</mi></mrow></math></span>, a common mode rejection ratio of over 96 dB, a measurement resolution of less than 0.1 <span><math><mrow><mi>μ</mi><mi>V</mi></mrow></math></span>, a bandwidth of 0.5 ∼ 45 Hz, and works at a sampling rate of 250 Hz. It also supports metal dry electrodes and includes a built-in analog bandpass filter, right-leg drive circuit, and built-in digital lowpass filter and notch filter, which can reduce noise during measurement. Researchers can reconstruct the electrode system to measure regions of interest according to their needs.</p></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"17 ","pages":"Article e00507"},"PeriodicalIF":2.2,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468067224000014/pdfft?md5=49b9e4de3c44905e67632156492470c4&pid=1-s2.0-S2468067224000014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139635652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0