{"title":"Open-source CNC workstation for paper-based diagnostic assay assembly","authors":"Lucy Tecle , Shannon Riegle , Andrew Piepho , Jacqueline Linnes","doi":"10.1016/j.ohx.2025.e00739","DOIUrl":"10.1016/j.ohx.2025.e00739","url":null,"abstract":"<div><div>Paper-based diagnostics are promising for point-of-care testing, but their assembly is often manual and can introduce alignment variability. To address this, we developed a low-cost, open-source workstation that repurposes a three-axis computer numerical control (CNC) machine for automated pick-and-place assembly of paper-based assays. The system integrates an Arduino-based controller with GRBL firmware, a custom vacuum end effector, and component holders to handle delicate assay components. This design eliminates reliance on proprietary CNC controls, reducing the costs to under $900 while enabling machine-agnostic adaptability. Performance was validated across dipstick, lateral flow immunoassay, and custom duplex immunoassay formats. Linear placement accuracy averaged ∼0.5–0.6 mm (within functional tolerance for diagnostic readability), while angular deviations (1–3°) remained acceptable for sample flow. Control line intensity in CNC-assembled assays were statistically indistinguishable from hand-assembled assays, confirming preserved diagnostic performance. By lowering the barrier to automated fabrication, this workstation provides an accessible platform for academic labs, startups, and decentralized environments to prototype and scale paper-based diagnostics. The open-source hardware and design files expand opportunities for reproducible, affordable diagnostic assembly in early-stage research and development.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"25 ","pages":"Article e00739"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2026-03-01Epub Date: 2025-12-13DOI: 10.1016/j.ohx.2025.e00729
A. Villarreal , J. Lazovic , S.E. Solis-Najera , R. Martin , R. Ruiz , L. Medina , A.O. Rodriguez
{"title":"A method for designing birdcage coils based on a simplified magnetic field model, validated experimentally at 4 T, 7 T, and 15.2 T","authors":"A. Villarreal , J. Lazovic , S.E. Solis-Najera , R. Martin , R. Ruiz , L. Medina , A.O. Rodriguez","doi":"10.1016/j.ohx.2025.e00729","DOIUrl":"10.1016/j.ohx.2025.e00729","url":null,"abstract":"<div><div>Magnetic resonance imaging and spectroscopy rely on magnetic fields generated by radiofrequency (RF) volume coils for high-quality data acquisition. Understanding electromagnetic field behavior in these coils is key to optimizing imaging and designing advanced coils. This paper presents an alternative tool to validate experimental and simulated results from traditional bridge coils using a theoretical approach to derive the magnetic field B1 expression for a BC coil. The formula will be used for: (a) assessing RF coil performance for high-quality images with optimal SNR and accurate anatomical representation, and (b) guiding the development of BC coils for specific applications. To validate the model, phantom images were acquired at different resonant frequencies, and the results were compared with experimental data. The findings confirm the accuracy and effectiveness of the model, offering insights into electromagnetic field behavior and providing a framework for advancing RF coil design in MR imaging and MR spectroscopy.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"25 ","pages":"Article e00729"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-11-24DOI: 10.1016/j.ohx.2025.e00725
Fox Avery , Thomas Geer , Dirk Albrecht
{"title":"Lensless light guide-coupled LED illumination for low-cost microscopy","authors":"Fox Avery , Thomas Geer , Dirk Albrecht","doi":"10.1016/j.ohx.2025.e00725","DOIUrl":"10.1016/j.ohx.2025.e00725","url":null,"abstract":"<div><div>Solid-state LED illumination systems for microscopy are generally expensive, often containing multiple LED drivers, excitation filters, and mirrors. High intensity illumination and precise timing are important for <em>in vivo</em> and <em>in vitro</em> microscopy, fluorescence imaging, and for optical stimulation. Here we present a low-cost, simplified single-wavelength LED mount with 3D printed components that achieves nearly 300 mW optical power through a plastic PMMA light guide, and 40–60 mW excitation intensity with ∼95 % flatfield uniformity at the sample, for under $70 USD. By keeping the LED light beam within the diameter of the light guide, no collecting or collimating lenses are needed. Illumination performance of the low-cost 3 W red and blue systems with passive cooling and plastic light guide match 7 W to 15 W commercial systems with active fan cooling and a liquid-filled light guide, while reducing cost over 20-fold. Additionally, low electrical power use (5 W) and small physical size enable its use in resource-limited environments and compact, battery-powered imaging devices.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00725"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-09-11DOI: 10.1016/j.ohx.2025.e00691
Cristian Castillo-Velásquez , Carlos Fuhrhop , Mario E. Flores , Sebastian Brauchi
{"title":"A cost-effective and open-source near-field electrospinning system with a graphical user interface","authors":"Cristian Castillo-Velásquez , Carlos Fuhrhop , Mario E. Flores , Sebastian Brauchi","doi":"10.1016/j.ohx.2025.e00691","DOIUrl":"10.1016/j.ohx.2025.e00691","url":null,"abstract":"<div><div>Electrospinning is a versatile technique widely used in biomedicine and electronics. Here we describe the design and construction of a low-cost Near-Field Electrospinning System (NFES) using open-source technologies, including 3D printing and open-source hardware and software. The system features a modified 3D printer for precise needle and mobile collector control, along with an Arduino-driven syringe pump to regulate the flow of the polymeric solution. A custom user interface ensures optimal conditions during operation. Proof-of-concept tests demonstrate the system capability to fabricate and functionalize microfibers using a polyethylene oxide solution in distilled water.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00691"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-10-01DOI: 10.1016/j.ohx.2025.e00710
Luis Corral, Pablo E. Román
{"title":"Design of a low-cost microphone array for portable multi-platform applications","authors":"Luis Corral, Pablo E. Román","doi":"10.1016/j.ohx.2025.e00710","DOIUrl":"10.1016/j.ohx.2025.e00710","url":null,"abstract":"<div><div>Acoustic imaging analysis of noise sources is a widespread method to obtain source spatial positioning and parameters like sound pressure. The challenge is to record audio from multiple microphones simultaneously and optionally capture color images from a camera for final overlapped sound localization display. We propose a multiple hardware component interconnection device, with necessary and optional parts available from different retailers and minimal printed circuit board designs. The setup is based on a 3D-printed base that can be modified to achieve different portable microphone array configurations. The final product is USB 2.0 compliant and can be connected to multiple computers and operating system, as well as various development boards. The simple script provided allows recording 16-channel files at 48 kHz and 32 bits, alongside optional colored point clouds from a depth camera. Measurements taken in an anechoic chamber and post-processing with inverse methods result in holographic reconstruction of noise sources surface parameters and a sound power approximation.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00710"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-10-15DOI: 10.1016/j.ohx.2025.e00716
Ryan T. Chaffer, Joseph Johnson, Matthew J. DiDomizio, Mark B. McKinnon
{"title":"Anaerobic Pyrolysis Chamber: An atmosphere-controlled furnace for controlled-atmosphere thermal decomposition","authors":"Ryan T. Chaffer, Joseph Johnson, Matthew J. DiDomizio, Mark B. McKinnon","doi":"10.1016/j.ohx.2025.e00716","DOIUrl":"10.1016/j.ohx.2025.e00716","url":null,"abstract":"<div><div>As some materials are heated they undergo thermal decomposition, resulting in a change in the material’s physical structure and chemical composition through a process called pyrolysis. During pyrolysis, flammable vapors are driven off the material; if those vapors are released in the presence of oxygen, flaming combustion may occur. Combustion can accelerate the decomposition process, making it difficult to isolate and harvest partially decomposed material samples for subsequent analysis. Due to this difficulty, the thermo-physical changes to materials undergoing pyrolysis have not been extensively studied. A new apparatus, termed the Anaerobic Pyrolysis Chamber (APC), was designed to produce gram-scale samples of partially decomposed materials by pyrolyzing those materials under a defined heating protocol and in an inert atmosphere. The APC consists of a decomposition chamber positioned within a muffle furnace, and an instrumentation package designed to monitor and control the temperature, pressure, and oxygen concentration within the decomposition chamber. The driving purpose of the APC was to produce solid products of pyrolysis for study in other measurement apparatus. The performance of the apparatus has been characterized, and the APC has been used to support property measurement in research studies.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00716"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145362740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-11-13DOI: 10.1016/j.ohx.2025.e00722
Nipuna Chamara , Yufeng Ge , Sabrina Russo
{"title":"FireLog: An open-source, low-cost system for temperature logging during wildland fires with high spatial and temporal resolution","authors":"Nipuna Chamara , Yufeng Ge , Sabrina Russo","doi":"10.1016/j.ohx.2025.e00722","DOIUrl":"10.1016/j.ohx.2025.e00722","url":null,"abstract":"<div><div>Measuring flame, air, and soil temperatures during wildland fires, including wildfires and controlled burns in land management contexts, is crucial for research and applications in fire ecology, safety, and management in a wide array of ecosystems, from grasslands to forests. However, open-source and commercial systems are needed for measuring and logging flame and air temperatures that are user-friendly, economical, modular, and customizable. This paper details the design, development, and validation of the FireLog system. Laboratory validation experiments demonstrated high measurement accuracy, with a minimum coefficient of determination (R2) of 0.98 and the highest observed root mean square error (RMSE) of 29.5 °C when compared with a Campbell Scientific data logger in furnace tests spanning 20–1000 °C. These results confirm the FireLog system’s precision, repeatability, and robustness under controlled conditions. Field deployment during prescribed burns further validated its operational performance, confirming its ability to record temperature dynamics reliably in active fire environments. FireLog represents a practical and scalable tool for researchers and practitioners in fire science and land management.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00722"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-09-19DOI: 10.1016/j.ohx.2025.e00701
Alejandro Gaviria-Cano, Cristian Escudero-Quintero, Jose David López-Suárez, Juan Pablo Villegas-Ceballos, Elkin Edilberto Henao-Bravo, Sergio Ignacio Serna-Garcés
{"title":"Cost-effective and versatile Hardware-in-the-Loop system for DC/DC converter emulation in education and research","authors":"Alejandro Gaviria-Cano, Cristian Escudero-Quintero, Jose David López-Suárez, Juan Pablo Villegas-Ceballos, Elkin Edilberto Henao-Bravo, Sergio Ignacio Serna-Garcés","doi":"10.1016/j.ohx.2025.e00701","DOIUrl":"10.1016/j.ohx.2025.e00701","url":null,"abstract":"<div><div>This work presents a cost-effective, versatile hardware-in-the-loop (HIL) system for non-isolated DC/DC converter emulation. The system emulates five types of converters (Buck, Boost, Buck-Boost, Cuk, and SEPIC) and targets educational and research applications. The hardware uses an ARM Cortex M7 microcontroller to perform real-time calculations and produce analog signals that emulate the behavior of the converters. Its economical, compact, and modular design is optimized to facilitate use in educational environments and research projects with limited resources. Additionally, the system includes software that enables automatic configuration of the code necessary for emulation, offering flexibility while reducing costs and complexity</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00701"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-10-27DOI: 10.1016/j.ohx.2025.e00718
Siluo Chen , Yu Chen , Jing Huang , Chengyu Liu , Kan Luo
{"title":"Open-source low-cost non-contact ECG monitoring system using active dry electrodes","authors":"Siluo Chen , Yu Chen , Jing Huang , Chengyu Liu , Kan Luo","doi":"10.1016/j.ohx.2025.e00718","DOIUrl":"10.1016/j.ohx.2025.e00718","url":null,"abstract":"<div><div>Continuous electrocardiogram (ECG) monitoring is essential for the early detection of cardiac arrhythmias and other heart conditions. However, conventional wet electrode-based systems often cause skin irritation and signal degradation, making them uncomfortable and unsuitable for long-term daily use. In this study, we present a fully open-source, low-cost (∼$14), miniaturized (∼50 × 35 × 28 mm<sup>3</sup>), and lightweight (∼60 g), wearable ECG monitoring platform that uses non-contact, active, dry electrodes based on capacitive coupling. The system integrates ultra-high input impedance electrode circuitry, an analog front-end integrated circuit, Bluetooth wireless communication, and an Android mobile application for real-time signal visualization and storage. Comprehensive validation shows that the system reliably acquires ECG signals through light to moderate clothing, with clear preservation of key features, such as QRS complexes, and accurate heart rate estimation. Comparative experiments with a commercial, clinical-grade ECG monitor demonstrate excellent agreement in rhythm tracking, and systematic module-level power analysis highlights further opportunities for low-power optimization. All hardware designs, firmware, and software are openly released to accelerate innovation and reproducibility in the field of wearable, non-contact ECG monitoring. The proposed platform provides a practical, extensible, and accessible reference for researchers aiming to advance next-generation cardiac monitoring technologies.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00718"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145416973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HardwareXPub Date : 2025-12-01Epub Date: 2025-10-04DOI: 10.1016/j.ohx.2025.e00709
Artur Schneider , Ilka Diester
{"title":"FreiLaser: Flexible pulse generator for laser control in optogenetic experiments","authors":"Artur Schneider , Ilka Diester","doi":"10.1016/j.ohx.2025.e00709","DOIUrl":"10.1016/j.ohx.2025.e00709","url":null,"abstract":"<div><div>To facilitate optogenetic experiments in neuroscience, we designed a cost-effective (<span><math><mo><</mo></math></span>40 €) and versatile laser control system, FreiLaser, based on the Raspberry Pi Pico microcontroller running CircuitPython. FreiLaser allows precise control over various stimulation parameters for up to four lasers, utilizing both analog and digital signals. It features a user-friendly graphical interface for parameter configuration and real-time visualization, as well as an API for seamless integration with existing experimental setups. The system also includes a built-in mask controller to prevent behavioral bias by synchronizing masking LEDs with laser pulses. Validation tests confirmed that FreiLaser generates stable, temporally precise control signals, suitable for a range of stimulation patterns. The system’s flexibility, ease of use, and low cost make it an invaluable tool for researchers conducting optogenetic and behavioral studies. Our open-source design ensures accessibility and adaptability for a wide range of experimental needs.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"24 ","pages":"Article e00709"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}