Current Opinion in Biomedical Engineering最新文献_第9页

To cut or not to cut: Next-generation genome editors for precision genome engineering 剪还是不剪:精确基因组工程的下一代基因组编辑器

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-07-07 DOI: 10.1016/j.cobme.2023.100489

Meng Zhang , Zhixin Zhu , Guanhua Xun , Huimin Zhao

{"title":"To cut or not to cut: Next-generation genome editors for precision genome engineering","authors":"Meng Zhang , Zhixin Zhu , Guanhua Xun , Huimin Zhao","doi":"10.1016/j.cobme.2023.100489","DOIUrl":"10.1016/j.cobme.2023.100489","url":null,"abstract":"<div><p>Since the original report of repurposing the CRISPR/Cas9 system for genome engineering, the past decade has witnessed profound improvement in our ability to efficiently manipulate the mammalian genome. However, significant challenges lie ahead that hinder the translation of CRISPR-based gene editing technologies into safe and effective therapeutics. The CRISPR systems often have a limited target scope due to PAM restrictions, and the off-target activity also poses serious risks for therapeutic applications. Moreover, the first-generation genome editors typically achieve desired genomic modifications by inducing double-strand breaks (DSBs) at target site(s). Despite being highly efficient, this “cut and fix” strategy is less favorable in clinical settings due to drawbacks associated with the nuclease-induced DSBs. In this review, we focus on recent advances that help address these challenges, including the engineering and discovery of novel CRISPR/Cas systems with improved functionalities and the development of DSB-free genome editors.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"28 ","pages":"Article 100489"},"PeriodicalIF":3.9,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10430874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anatomics: Co-engineering body and machine in pursuit of synergistic bionic performance 解剖学：身体和机器的协同工程，追求协同仿生性能

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-07-07 DOI: 10.1016/j.cobme.2023.100490

Tyler R. Clites

引用次数: 0

Current opinion: Underreported cell populations in the aortic valve influence tissue development and homeostasis 当前观点-主动脉瓣中未充分报道的细胞群影响组织发育和稳态

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-07-04 DOI: 10.1016/j.cobme.2023.100486

Daniel Chaparro , Joshua D. Hutcheson

{"title":"Current opinion: Underreported cell populations in the aortic valve influence tissue development and homeostasis","authors":"Daniel Chaparro , Joshua D. Hutcheson","doi":"10.1016/j.cobme.2023.100486","DOIUrl":"10.1016/j.cobme.2023.100486","url":null,"abstract":"<div><p>During valvulogenesis, various cells in the developing heart remodel the cushions in the outflow tract to shape the aortic valve (AoV). In adulthood, mature cells maintain the tissue in homeostatic balance to cope with changes in cardiac mechanics. Pathological progression of aortic valve disease is characterized by asymptomatic irreversible extracellular matrix (ECM) remodeling rendering the valve dysfunctional, ultimately leading to heart failure and death if the valve is not replaced. Resident cells within the tissue are responsible for congenital and acquired pathological conditions. A complex heterogenous population of cells resides within the valve apparatus, including underreported populations of neurons and melanocytes. Though not thoroughly explored, reports suggest that these cell types have implications in development, homeostasis, and disease progression within the AoV. In this current opinion piece, we summarize recent and relevant literature exploring the connection between both AoV cellular heterogeneity and cell developmental origin in development and disease.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"28 ","pages":"Article 100486"},"PeriodicalIF":3.9,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41973653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modular and handheld Raman systems for SERS-based point-of-care diagnostics 模块化和手持式拉曼系统为基于sers的点护理诊断

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-07-04 DOI: 10.1016/j.cobme.2023.100488

Sayantan Tripathy , Suhash Chavva , Gerard L. Coté , Samuel Mabbott

引用次数: 0

Towards ultrasound imaging-based closed-loop peripheral nerve stimulation for tremor suppression 基于超声成像的闭环周围神经刺激抑制震颤

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-06-24 DOI: 10.1016/j.cobme.2023.100484

Nitin Sharma , Xiangming Xue , Ashwin Iyer , Xiaoning Jiang , Daniel Roque

引用次数: 0

Intelligent biomaterials for cardiovascular applications 用于心血管应用的智能生物材料

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-06-22 DOI: 10.1016/j.cobme.2023.100474

S.S.V. Tetali, A.T.R. Fricker, Y.A. van Domburg, I. Roy

{"title":"Intelligent biomaterials for cardiovascular applications","authors":"S.S.V. Tetali, A.T.R. Fricker, Y.A. van Domburg, I. Roy","doi":"10.1016/j.cobme.2023.100474","DOIUrl":"10.1016/j.cobme.2023.100474","url":null,"abstract":"<div><p>Cardiovascular disease remains a leading cause of morbidity and mortality worldwide, and as such, research in cardiovascular medicine is continuously evolving. Recent advances in technology have created opportunities for improving the diagnosis and management of cardiovascular disease. This review article summarizes the use of innovative polymeric biomaterials for various cardiovascular applications, highlighting promising results obtained in the past five years.</p><p>The review begins by discussing the use of artificial blood vessels and coronary artery stents with biosensors for coronary artery disease management. Additionally, the studies on cardiac patches for heart failure management are evaluated. The review also covers recent advancements in artificial intelligence and real-time health monitoring for diagnosing cardiovascular conditions such as arrhythmias and structural heart disease. New catheters for epicardial mapping and stretchable conducting polymers for surface electrodes have improved diagnostic capabilities. The review also examines advancements in engineering with intelligent biomaterials for unique and sustainable treatment options. This includes piezoelectric and triboelectric nanogenerators for improved cardiovascular devices, reducing the need for battery changes and the risk of infections.</p><p>Overall, the review provides a comprehensive analysis of innovative polymeric biomaterials for various cardiovascular diagnostic and treatment modalities. It summarizes recent studies that demonstrate the potential of these materials for improving patient outcomes and ultimately reducing the burden of cardiovascular disease. As the field of cardiovascular medicine continues to evolve, these advancements may pave the way for further progress in the diagnosis and management of cardiovascular disease.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"28 ","pages":"Article 100474"},"PeriodicalIF":3.9,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45077881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterizing off-target effects of genome editors 描述基因组编辑的脱靶效应

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-06-17 DOI: 10.1016/j.cobme.2023.100480

Xichen Rao , Huanan Zhao , Chuyun Shao , Chengqi Yi

引用次数: 3

Detection and quantification of unintended large on-target gene modifications due to CRISPR/Cas9 editing CRISPR/Cas9编辑引起的非预期大靶基因修饰的检测和定量

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-06-16 DOI: 10.1016/j.cobme.2023.100478

So Hyun Park, Mingming Cao, Gang Bao

引用次数: 2

Surface modifications of nanocellulose for drug delivery applications; a critical review 用于药物递送应用的纳米纤维素的表面改性；批判性评论

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-06-08 DOI: 10.1016/j.cobme.2023.100475

Muhammad Mujtaba , Arvind Negi , Alistair W.T. King , Mina Zare , Johana Kuncova-Kallio

{"title":"Surface modifications of nanocellulose for drug delivery applications; a critical review","authors":"Muhammad Mujtaba , Arvind Negi , Alistair W.T. King , Mina Zare , Johana Kuncova-Kallio","doi":"10.1016/j.cobme.2023.100475","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100475","url":null,"abstract":"<div><p>With the development of novel drugs and the discovery of new targets, there is an increased need for novel drug carriers. Nanocellulose has great potential to become a novel drug carrier thanks to its biocompatibility, low toxicity, tunable chemical structure, and sustainability. There are many types of nanocelluloses based on their physical properties (particle size, shape, surface charge), chemical properties (surface functional groups), form (lyophilized, hydrogel, aerogel, membrane) and source (bacterial, animal, plant) and that also largely influences drug release patterns, but also toxicity profile. To our knowledge, so far, no nanocellulose materials have been accepted as ingredients for pharmaceutical formulations. Some types of nanocellulose have however been accepted for use in medical devices, which is also a tightly regulated area. This review focuses on the modification of nanocellulose to enhance its capability as a drug delivery agent and pinpoints the pitfalls for the registration of such novel sustainable material.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"28 ","pages":"Article 100475"},"PeriodicalIF":3.9,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49817095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolutionizing genetic disease treatment: Recent technological advances in base editing 革命性的遗传疾病治疗:碱基编辑的最新技术进展

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-06-02 DOI: 10.1016/j.cobme.2023.100472

Tyler C. Daniel , Hongzhi Zeng , Emmanuel C. Osikpa , Xue Gao

引用次数: 2