发布求助
文献互助 智能选刊 最新文献

Current Opinion in Biomedical Engineering最新文献

筛选
英文 中文
Protein engineering technologies for development of next-generation genome editors 开发下一代基因组编辑器的蛋白质工程技术
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-11-01 DOI: 10.1016/j.cobme.2023.100514
Jackson Winter , Shraddha Shirguppe , Pablo Perez-Pinera
{"title":"Protein engineering technologies for development of next-generation genome editors","authors":"Jackson Winter ,&nbsp;Shraddha Shirguppe ,&nbsp;Pablo Perez-Pinera","doi":"10.1016/j.cobme.2023.100514","DOIUrl":"10.1016/j.cobme.2023.100514","url":null,"abstract":"<div><p><span>Base editors and prime editors have emerged as promising tools for the modeling and treatment of genetic diseases due to their ability to introduce targeted modifications in the </span>genomic DNA<span> of living cells. Several engineering approaches have been applied to improve their performance, ranging from simple protein design<span> approaches to complex directed evolution schemes that can probe a vast landscape of mutational variants with minimal user intervention. These extensive efforts have led to new generations of editors with enhanced properties such as increased editing activity, tailored editing windows, increased targetability, smaller construct size for viral delivery, and decreased off-target effects. In this manuscript we review protein engineering technologies that have been recently utilized to create an ever-evolving landscape of high-performance gene editing tools specifically designed for genetic targets of interest and that have redefined what is possible in the field of precision medicine.</span></span></p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135222101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-use biosensors for biomanufacturing: Perspective on the state-of-the-art 用于生物制造的一次性生物传感器:最先进的观点
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-10-31 DOI: 10.1016/j.cobme.2023.100512
Zach Hetzler , Noah Lott , Aditi Dey Poonam , Selen Dalgan , Qingshan Wei
{"title":"Single-use biosensors for biomanufacturing: Perspective on the state-of-the-art","authors":"Zach Hetzler ,&nbsp;Noah Lott ,&nbsp;Aditi Dey Poonam ,&nbsp;Selen Dalgan ,&nbsp;Qingshan Wei","doi":"10.1016/j.cobme.2023.100512","DOIUrl":"10.1016/j.cobme.2023.100512","url":null,"abstract":"<div><p>The biomanufacturing industry has experienced significant transformations in the past 10 years, driven by changing industry trends and rapidly maturing new technologies. However, the pace of process monitoring technology development has lagged behind. This review focuses on the current major gaps in critical monitoring technology required for industry advancement, with a particular focus on single-use biosensors. There is a large unmet need for single-use biosensors in upstream and downstream processing<span> as the industry transitions to single-use bioreactors. Emerging fields like cell and gene therapy that are still lacking numerous satisfactory at-line product characterization sensors will also be covered.</span></p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microfluidic platforms based on SERS imaging and their point-of-care applications 基于SERS成像的微流控平台及其护理点应用
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-10-27 DOI: 10.1016/j.cobme.2023.100510
Hilal Torul , Emine Yıldırım , Uğur Tamer
{"title":"Microfluidic platforms based on SERS imaging and their point-of-care applications","authors":"Hilal Torul ,&nbsp;Emine Yıldırım ,&nbsp;Uğur Tamer","doi":"10.1016/j.cobme.2023.100510","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100510","url":null,"abstract":"<div><p>Surface-enhanced Raman spectroscopy (SERS) imaging is one of the most prevalent and efficient molecular imaging techniques that may provide enhanced Raman signals of samples such as cells or biomolecules for highly accurate measurement. Furthermore, SERS imaging-based techniques can be adapted to portable systems, making them sufficient to be used for POC applications. In this review, we focused on prominent publications based on SERS imaging techniques in combination with microfluidics platforms for POC analysis of proteins, biomarkers and pathogens over the past five years.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The changing landscape of gene editing tool delivery in neurological disorders 神经系统疾病中基因编辑工具传递的变化前景
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-10-12 DOI: 10.1016/j.cobme.2023.100509
Yu-Ju Chen, Abhik Paul, Michael Gregory Collins, Hye Young Lee
{"title":"The changing landscape of gene editing tool delivery in neurological disorders","authors":"Yu-Ju Chen,&nbsp;Abhik Paul,&nbsp;Michael Gregory Collins,&nbsp;Hye Young Lee","doi":"10.1016/j.cobme.2023.100509","DOIUrl":"10.1016/j.cobme.2023.100509","url":null,"abstract":"<div><p>The delivery of gene editing tools has made significant advancements in recent years. This being said, the brain remains a challenging organ for the safe and efficient delivery of gene editing tools. As nonviral delivery vehicles have shown promise with their safety profiles and biocompatibility, there have been efforts to test nonviral delivery vehicles for gene editing tools in the brain. Here, we review recent advancements in gene editing tool development and discuss how they were tested using nonviral delivery vehicles, with a specific focus on brain applications.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135706116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biomaterials: Intelligent biomaterials 2023 生物材料:智能生物材料2023
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-10-06 DOI: 10.1016/j.cobme.2023.100508
Himansu Sekhar Nanda, Masoud Mozafari, Gulden Camci-Unal, Seeram Ramakrishna, Ravin Narain
{"title":"Biomaterials: Intelligent biomaterials 2023","authors":"Himansu Sekhar Nanda,&nbsp;Masoud Mozafari,&nbsp;Gulden Camci-Unal,&nbsp;Seeram Ramakrishna,&nbsp;Ravin Narain","doi":"10.1016/j.cobme.2023.100508","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100508","url":null,"abstract":"","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92042580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Futures of BME: Sustainable medical materials 2023 BME的未来:可持续医疗材料2023
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-09-28 DOI: 10.1016/j.cobme.2023.100507
Seeram Ramakrishna, Aldo R. Boccaccini, Mina Zare
{"title":"Futures of BME: Sustainable medical materials 2023","authors":"Seeram Ramakrishna,&nbsp;Aldo R. Boccaccini,&nbsp;Mina Zare","doi":"10.1016/j.cobme.2023.100507","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100507","url":null,"abstract":"","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468451123000636/pdfft?md5=f7d7257320e223763cf7ef68bbf87295&pid=1-s2.0-S2468451123000636-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91956957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fueling next-generation genome editing with DNA repair 通过DNA修复推动下一代基因组编辑
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-09-22 DOI: 10.1016/j.cobme.2023.100506
Ana Gvozdenovic, Jacob E. Corn
{"title":"Fueling next-generation genome editing with DNA repair","authors":"Ana Gvozdenovic,&nbsp;Jacob E. Corn","doi":"10.1016/j.cobme.2023.100506","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100506","url":null,"abstract":"<div><p>Genome editing technologies generate targeted DNA lesions and rely on cellular DNA repair pathways for resolution. Understanding the DNA repair mechanisms responsible for resolving the specific damage caused by gene editing tools can significantly advance their optimization and facilitate their broader application in research and therapeutic contexts. Here we explore the cellular processes involved in repairing base and prime editor-generated DNA lesions and strategies to leverage and manipulate DNA repair pathways for desired genomic changes.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468451123000624/pdfft?md5=406dc88a0f0e1ffc1c4e21a01f36d737&pid=1-s2.0-S2468451123000624-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91956956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-invasive brain imaging to advance the understanding of human balance 无创大脑成像促进对人类平衡的理解
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-09-16 DOI: 10.1016/j.cobme.2023.100505
Helen J. Huang , Daniel P. Ferris
{"title":"Non-invasive brain imaging to advance the understanding of human balance","authors":"Helen J. Huang ,&nbsp;Daniel P. Ferris","doi":"10.1016/j.cobme.2023.100505","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100505","url":null,"abstract":"<div><p>Humans depend on mobility for social interaction, cognitive development, and health maintenance. Successful mobility requires maintaining balance, which integrates sensory feedback, internal cognitive models of body dynamics, and musculoskeletal actions. There have been great strides in understanding these components of balance control in the last 20 years, but balance deficits persist in a large percentage of the population. We propose that combining non-invasive brain imaging using high-density electroencephalography (EEG) with behavioral and biomechanical measures could reveal unique insights about balance control. Source separation and localization of brain electrical activity during mobile tasks have improved with advancements in electrodes and motion artifact removal. This enables studying naturally occurring balance tasks with and without perturbations to identify the timing, magnitude, and quality of brain processing during balance. Along with efforts toward more inclusive EEG research and open resources, this approach could help diagnose and treat poor balance ability among more people.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49817084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanotechnology-based strategies for advancing point-of-care lateral flow immunoassays 基于纳米技术的策略推进护理点横向流免疫测定
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-09-09 DOI: 10.1016/j.cobme.2023.100504
Peuli Nath, Aniruddha Ray
{"title":"Nanotechnology-based strategies for advancing point-of-care lateral flow immunoassays","authors":"Peuli Nath,&nbsp;Aniruddha Ray","doi":"10.1016/j.cobme.2023.100504","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100504","url":null,"abstract":"<div><p>Point-of-care tests (POCTs) have the potential to transform medical diagnostics by providing rapid and accurate results at the site of patient care. Of the many different types of POCTs being developed, lateral flow immunoassays (LFIAs) are some of the most widely used due to their simplicity, low cost, and fast response time. Combining recent advances in nanotechnology with the LFIAs has drastically improved their performance in terms of their sensitivity, specificity, and limit of detection, as well as their long-term stability. Nanoparticles, both plasmonic and fluorescent, can be functionalized with antibodies/aptamers and other targeting moieties, thereby enabling the detection of a wide range of biomarkers. Here we discuss various strategies, utilizing nanotechnology, to enhance the performance of these LFIAs in order to break the limits of the currently used conventional assays. Successful deployment of these tests will facilitate widespread use, thereby revolutionizing the current diagnostic and treatment practices, as well as curb future pandemics. Extensive utilization of these tests will also reduce the high medical expenditures, which is a central object of health care reform in every country.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49817085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using biophysical cues and biomaterials to improve genetic models 利用生物物理线索和生物材料改进遗传模型
IF 3.9 3区 工程技术
Current Opinion in Biomedical Engineering Pub Date : 2023-09-09 DOI: 10.1016/j.cobme.2023.100502
Thomas G. Molley , Adam J. Engler
{"title":"Using biophysical cues and biomaterials to improve genetic models","authors":"Thomas G. Molley ,&nbsp;Adam J. Engler","doi":"10.1016/j.cobme.2023.100502","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100502","url":null,"abstract":"<div><p>With the advent of induced pluripotent stem cells and modern differentiation protocols, many advances in our understanding of disease have been made possible by <em>in vitro</em> disease modeling; in some cases, their use may have supplanted animal models. Yet <em>in vitro</em> models often rely on rigid cell culture substrates that could limit our ability to completely reproduce human disease in a dish. Nascent work, however, suggests that the combination of biomaterials and/or advanced microphysiological systems–which better recapitulate tissue properties–with stem cells expressing disease mimicking genetics, could substantially improve current disease modeling efforts where genetics alone is insufficient. This review will highlight such recent advances as well as review current challenges that the fields must overcome to create more personalized therapeutics in the future.</p></div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49857656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信