Current Opinion in Biomedical Engineering最新文献_第4页

3D bioprinting strategies for recapitulation of hepatic structure and function in bioengineered liver: A state-of-the-art review 在生物工程肝脏中再现肝脏结构和功能的三维生物打印策略：最新进展综述

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2024-02-13 DOI: 10.1016/j.cobme.2024.100526

Arka Sanyal, Sourabh Ghosh

{"title":"3D bioprinting strategies for recapitulation of hepatic structure and function in bioengineered liver: A state-of-the-art review","authors":"Arka Sanyal, Sourabh Ghosh","doi":"10.1016/j.cobme.2024.100526","DOIUrl":"10.1016/j.cobme.2024.100526","url":null,"abstract":"<div>3D bioprinting has recently emerged as a successful biofabrication strategy for replicating the complex in vivo hepatic milieu. Significant research advances in this field have allowed for the fabrication of biomimetic hepatic tissues with potential applications in the healthcare (regeneration, transplantation, drug discovery) and diagnostic sectors (in vitro disease models). This article initially delves into describing the hepatic tissue architecture and function, followed by a rational exposition of how 3D bioprinting potentiates the better development of functional liver tissue compared to traditional tissue engineering approaches and 3D cell culture platforms. This review then highlights the recent breakthroughs and reliable strategies for replicating liver structure and function through bioprinting approaches. In this context, we have systematically described the current landscape of hepatic bioprinting, initially focusing on the cell sources used, followed by the biomaterials and strategies implemented to prolong their in vitro viability. Proceeding forward, we have critically highlighted essential aspects of hepatic bioprinting, such as developing tissue-specific bioinks, strategies to induce vascularization within bioprinted liver constructs, and replication of native liver tissue heterogeneity through spatial distribution of multiple cell types in predetermined patterns. In our concluding remarks, we discuss the existing bottlenecks that prevail in this field and provide our viewpoint regarding possible future directions to overcome them.</div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"30 ","pages":"Article 100526"},"PeriodicalIF":3.9,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139891823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial overview: Low-cost and portable systems for biomedical imaging and sensing 编辑综述：用于生物医学成像和传感的低成本便携式系统

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2024-02-09 DOI: 10.1016/j.cobme.2024.100527

Hatice Ceylan Koydemir, Aydogan Ozcan

引用次数: 0

Cell therapy for duchenne muscular dystrophy using induced pluripotent stem cell-derived muscle stem cells and the potential of regenerative rehabilitation 利用诱导多能干细胞衍生的肌肉干细胞治疗杜兴氏肌肉萎缩症的细胞疗法和再生康复的潜力

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2024-02-02 DOI: 10.1016/j.cobme.2024.100523

Nana Takenaka-Ninagawa , Megumi Goto , Clémence Kiho Bourgeois Yoshioka , Mayuho Miki , Hidetoshi Sakurai

引用次数: 0

Biophysical determinants of nuclear shape and mechanics and their implications for genome integrity 核形状和力学的生物物理决定因素及其对基因组完整性的影响

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2024-01-26 DOI: 10.1016/j.cobme.2024.100521

S. Hervé, Y.A. Miroshnikova

引用次数: 0

Editorial overview: The next-generation of genome editing: The future is now 新一代基因组编辑：未来就在眼前

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2024-01-23 DOI: 10.1016/j.cobme.2024.100522

Pablo Perez-Pinera, Thomas Gaj

引用次数: 0

AAV-based CRISPR-Cas9 genome editing: Challenges and engineering opportunities 基于 AAV 的 CRISPR-Cas9 基因组编辑：挑战与工程机遇

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-12-07 DOI: 10.1016/j.cobme.2023.100517

Ami M. Kabadi , Maria Katherine Mejia-Guerra , John D. Graef , Sohrab Z. Khan , Eric M. Walton , Xinzhu Wang , Charles A. Gersbach , Rachael Potter

{"title":"AAV-based CRISPR-Cas9 genome editing: Challenges and engineering opportunities","authors":"Ami M. Kabadi , Maria Katherine Mejia-Guerra , John D. Graef , Sohrab Z. Khan , Eric M. Walton , Xinzhu Wang , Charles A. Gersbach , Rachael Potter","doi":"10.1016/j.cobme.2023.100517","DOIUrl":"10.1016/j.cobme.2023.100517","url":null,"abstract":"<div>Recent innovations in the field of gene therapy have paved the way for advances towards developing genome editing medicines. Despite these steps forward, challenges with viral delivery of genome editing tools persist. Efforts currently underway include developing next-generation genome editors, overcoming adeno-associated virus (AAV) packaging restrictions, improving AAV genome integrity, engineering novel AAV capsids, and minimizing the immune response. This review discusses current challenges in delivering CRISPR-Cas nuclease-based genome editing therapies using AAV and highlights emerging methods to overcome these obstacles. This includes developing smaller payloads and regulatory elements, advancing novel sequencing methods for vector characterization, engineering capsids with enhanced potency, tissue-selectivity, and ability to evade pre-existing antibodies, controlling transgene expression, and minimizing the immune response to Cas proteins.</div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"29 ","pages":"Article 100517"},"PeriodicalIF":3.9,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468451123000739/pdfft?md5=23fd7e302b12d0dee1de084f88f1a6ed&pid=1-s2.0-S2468451123000739-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138617634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new function for nuclear lamins: providing surface tension to the nuclear drop. 核片层蛋白的新功能：为核滴提供表面张力。

IF 4.7 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-12-01 Epub Date: 2023-06-20 DOI: 10.1016/j.cobme.2023.100483

Richard B Dickinson, Tanmay P Lele

{"title":"A new function for nuclear lamins: providing surface tension to the nuclear drop.","authors":"Richard B Dickinson, Tanmay P Lele","doi":"10.1016/j.cobme.2023.100483","DOIUrl":"10.1016/j.cobme.2023.100483","url":null,"abstract":"The nuclear lamina, a conserved structure in metazoans, provides mechanical rigidity to the nuclear envelope. A decrease in lamin levels and/or lamin mutations are associated with a host of human diseases. Despite being only about 15 nm thick, perturbation of components of the nuclear lamina dramatically impacts the deformation response of the entire nucleus through mechanisms that are not well understood. Here we discuss evidence for the recently proposed 'nuclear drop' model that explains the role of A-type lamins in nuclear deformation in migrating cells. In this model, the nuclear lamina acts as an inextensible surface, supporting a surface tension when fully unfolded, that balances nuclear interior pressure. Much like a liquid drop surface where the molecularly thin interface governs surface tension and drop shape under external forces, the thin nuclear lamina imparts a surface tension on the nuclear drop to resist nuclear deformation as well as to establish nuclear shape. We discuss implications of the nuclear drop model for the function of this crucially important eukaryotic organelle.","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"1 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10812902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54234085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the targets of therapeutic electrophysical stimulation - For the advancement of peripheral nerve regenerative rehabilitation 扩大治疗性电物理刺激的靶点——促进周围神经再生康复

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-12-01 DOI: 10.1016/j.cobme.2023.100515

Shixuan Xu, Akira Ito

引用次数: 0

Pooled screening with next-generation gene editing tools. 利用新一代基因编辑工具进行联合筛选。

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-12-01 Epub Date: 2023-06-21 DOI: 10.1016/j.cobme.2023.100479

Liqun Zhou, Luojia Yang, Yanzhi Feng, Sidi Chen

引用次数: 0

Mechanome-guided strategies in regenerative rehabilitation 再生康复的机制引导策略

IF 3.9 3区工程技术

Current Opinion in Biomedical Engineering Pub Date : 2023-11-30 DOI: 10.1016/j.cobme.2023.100516

Diego Jacho, Eda Yildirim-Ayan

{"title":"Mechanome-guided strategies in regenerative rehabilitation","authors":"Diego Jacho, Eda Yildirim-Ayan","doi":"10.1016/j.cobme.2023.100516","DOIUrl":"https://doi.org/10.1016/j.cobme.2023.100516","url":null,"abstract":"<div>Regenerative Rehabilitation represents a multifaceted approach that merges mechanobiology with therapeutic intervention to harness the body's intrinsic tissue repair and regeneration capacity. This review delves into the intricate interplay between mechanical loading and cellular responses in the context of musculoskeletal tissue healing. It emphasizes the importance of understanding the phases involved in translating mechanical forces into biochemical responses at the cellular level. The review paper also covers the mechanosensitivity of macrophages, fibroblasts, and mesenchymal stem cells, which play a crucial role during regenerative rehabilitation since these cells exhibit unique mechanoresponsiveness during different stages of the tissue healing process. Understanding how mechanical loading amplitude and frequency applied during regenerative rehabilitation influences macrophage polarization, fibroblast-to-myofibroblast transition (FMT), and mesenchymal stem cell differentiation is crucial for developing effective therapies for musculoskeletal tissues. In conclusion, this review underscores the significance of mechanome-guided strategies in regenerative rehabilitation. By exploring the mechanosensitivity of different cell types and their responses to mechanical loading, this field offers promising avenues for accelerating tissue healing and functional recovery, ultimately enhancing the quality of life for individuals with musculoskeletal injuries and degenerative diseases.</div>","PeriodicalId":36748,"journal":{"name":"Current Opinion in Biomedical Engineering","volume":"29 ","pages":"Article 100516"},"PeriodicalIF":3.9,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138769743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0