Journal of Equine Science最新文献_第4页

A case of equine piroplasmosis in the Tokyo 2020 Olympic Games. 2020年东京奥运会上的一例马梨状体病。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.93

Hiroko Aida, Jonathan H Foreman, Akihiro Ochi, Yoshimasa Takizawa, Takashi Yamanaka

引用次数: 0

Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests. 适合基因兴奋剂检测的血浆样品的最佳储存和使用程序的研究。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.21

Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Yuji Takahashi, Shun-Ichi Nagata

{"title":"Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests.","authors":"Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Yuji Takahashi, Shun-Ichi Nagata","doi":"10.1294/jes.34.21","DOIUrl":"https://doi.org/10.1294/jes.34.21","url":null,"abstract":"Gene doping, which is prohibited in horseracing and equestrian sports, can be performed by introducing exogenous genes, known as transgenes, into the bodies of postnatal animals. To detect exogenous genes, a method utilizing quantitative polymerase chain reaction (qPCR) with a hydrolysis probe was developed to test whole blood and plasma samples, thereby protecting the fairness of competition and the rights of stakeholders in horseracing and equestrian sports. Therefore, we aimed to develop sample storage methods suitable for A and B samples in gene doping tests using blood. For sample A, sufficient qPCR detection was demonstrated after refrigeration for 1 to 2 weeks post collection. For sample B, the following procedures were confirmed to be suitable for storage: 1) centrifugation after sample receipt, 2) frozen storage, 3) natural thawing at room temperature, and 4) centrifugation without mixing blood cell components. Our results indicated that long-term cryopreservation yielded good plasma components from frozen blood samples even though it destroyed blood cells, indicating its applicability to the gene doping test using sample B, which can be stored for later use. Sample storage procedures are as important as detection methods in doping tests. Therefore, the series of procedures that we evaluated in this study will contribute to the efficient performance of gene doping tests through qPCR using blood samples.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 2","pages":"21-27"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e3/c6/jes-34-021.PMC10315635.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunohistochemical markers for equine granulosa cell tumors: a pilot study. 马颗粒细胞肿瘤的免疫组织化学标记物:一项初步研究。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.37

Aurelia Dolin, Peter Schweiger, Martin Waselau, Monika Egerbacher, Ingrid Walter

引用次数: 0

Efficiency of round bale feeders: comparison of Tombstone versus Hay Saver. 圆捆喂料机的效率:墓碑与干草节省器的比较。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.51

Kendall A Hyde, Annabelle Altman, Robin Banasek, Melba O Gastal, Eduardo L Gastal

引用次数: 0

Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses. 输血供马抗红细胞抗体阳性转化的监测。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.47

Hironaga Kakoi, Mio Kikuchi, Taichiro Ishige, Yuko Hirosawa, Shoko Tanaka, Shun-Ichi Nagata

{"title":"Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses.","authors":"Hironaga Kakoi, Mio Kikuchi, Taichiro Ishige, Yuko Hirosawa, Shoko Tanaka, Shun-Ichi Nagata","doi":"10.1294/jes.34.47","DOIUrl":"https://doi.org/10.1294/jes.34.47","url":null,"abstract":"To confirm the positive conversion of antibodies against erythrocyte antigens in horses, possible blood transfusion donor horses selected from draft horse populations were periodically monitored with an indirect antiglobulin (Coombs) test for approximately 3 years. In this study, 19 horses (16 females and 3 males) were investigated, and five mares showed alloantibodies during the monitoring period. Four mares were typically pregnant when positive conversion was detected, whereas no particular cause of conversion could be observed for one mare based on its clinical records. In the analyzed horses, most positive conversions were possibly due to pregnancy, as conversion occurred more often during this period than after parturition. Pregnancy is considered a key event for positive conversion. Additionally, in cases in which unknown causative sensitization is confirmed, continuous monitoring with a test to detect antibodies should be performed, even if the possible donor is selected and maintained.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 2","pages":"47-49"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/91/cf/jes-34-047.PMC10315640.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10160532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of equine metabolic syndrome, clinical manifestations, and associated risk factors in Nigeria. 尼日利亚马代谢综合征的发生、临床表现及相关危险因素

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.29

Olumide Odunayo Akinniyi, Anthony Kojo Beku Sackey, Gabriel Enenche Ochube, Philip Wayuta Mshelia, Francis Alkali Musa, Mary Oluwatomisin Elijah, Kelvin Olutimilehin Jolayemi

{"title":"Occurrence of equine metabolic syndrome, clinical manifestations, and associated risk factors in Nigeria.","authors":"Olumide Odunayo Akinniyi, Anthony Kojo Beku Sackey, Gabriel Enenche Ochube, Philip Wayuta Mshelia, Francis Alkali Musa, Mary Oluwatomisin Elijah, Kelvin Olutimilehin Jolayemi","doi":"10.1294/jes.34.29","DOIUrl":"https://doi.org/10.1294/jes.34.29","url":null,"abstract":"Insulin dysregulation (ID) is central to the pathophysiology of equine metabolic syndrome (EMS), putting the horse at risk of laminitis. There is a paucity of information on the status of EMS in Nigeria. This study aimed to determine the occurrence of EMS, clinical manifestations, and associated risk factors in Nigeria. A cross-sectional study was carried out. Selected horses underwent an insulin 2-step response test to ascertain insulin dysregulation; a physical examination was carried out to diagnose laminitis and obesity. Risk factors were assessed using a questionnaire. The overall prevalence of EMS was 43.10%. Breed and sex were significantly associated with EMS, but age was not. Horses diagnosed with laminitis showed two signs of laminitis, namely, divergent hoof rings and widened white lines. Risk factors significantly associated with the prevalence of EMS were as follows: being a West African Barb horse (60.00%), being a stallion (67.86%), being a leisure horse (67.86%), only walking horses during exercise (68.00%), exercising horses once every 5 months (82.76%), tethering horses to a stake in the ground (67.86%), obesity (92.86%), and abnormal neck crest (83.33%). The risk of ID remains higher in obese horses. However, some of the horses with ID were not obese, indicating that there are other possible underlying causes of EMS.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 2","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fd/d6/jes-34-029.PMC10315637.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9858301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Preliminary study of heart rate variability in Criollo horses for the elucidation of their neurophysiological characteristics of autonomic nerve function. 马心率变异性的初步研究，以阐明其自主神经功能的神经生理特征。

Journal of Equine Science Pub Date : 2023-06-01 DOI: 10.1294/jes.34.55

Nozomi Seki, Ryota Tochinai, Shin-Ichi Sekizawa, Martín Alejandro Márquez, Kentaro Fukuda, Hajime Ohmura, Masayoshi Kuwahara

引用次数: 0

Enhanced detection of Taylorella equigenitalis by qPCR using 'Dry' swabs. 用“干”拭子增强qPCR检测均匀泰勒菌。

Journal of Equine Science Pub Date : 2023-03-01 DOI: 10.1294/jes.34.7

Ian Mawhinney, Anne Bollard

{"title":"Enhanced detection of Taylorella equigenitalis by qPCR using 'Dry' swabs.","authors":"Ian Mawhinney, Anne Bollard","doi":"10.1294/jes.34.7","DOIUrl":"https://doi.org/10.1294/jes.34.7","url":null,"abstract":"Detection of Taylorella equigenitalis (CEMO) in the horse uses genital swabs. These swabs traditionally have been put in Amies charcoal transport medium for detection by culture but are also used for PCR. We determined the suitability of swabs without transport medium (Dry swabs) for CEMO PCR compared to swabs in Amies charcoal transport medium. The experiment was a factorial design using swab type and dilution of organism in culture suspensions, done in two parts. Simulated genital swabs were prepared in the laboratory by dipping in pairs into culture suspensions containing T. equigenitalis with or without other organisms, and then inserting them into a sleeve either with or without transport medium. In study 1, the difference in Ct value for the two swab types was compared. In study 2 genital swab material was then also added to culture suspensions and the swab types again compared. The swabs were tested by a validated quantitative PCR method. The Ct value of the PCR test was used as the measure for comparison, and the effect of variables assessed with linear regression. There was an 7.7% (6.5-8.9) higher mean Ct value of TM versus Dry swabs (P<0.001) overall. The Ct difference was more marked at higher dilutions. Addition of genital swab material had no effect on the Ct value. Dry swabs perform at least as well for PCR as swabs in Amies charcoal transport medium, especially when relatively low numbers of organism are present, and are advantageous for routine sampling when culture is not being used.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 1","pages":"7-12"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e1/22/jes-34-007.PMC10122987.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of genetic diversity using 31 microsatellites in Miyako horses. 利用31颗微卫星评价宫古马遗传多样性。

Journal of Equine Science Pub Date : 2023-03-01 DOI: 10.1294/jes.34.1

Mioko Masuda, Akihiro Nikadori, Emi Nikadori, Naoya Uchida, Yoshimasa Takizawa, Mutsuki Ishimaru, Takumi Yamamoto, Reiko Yuasa, Haruo Kugai, Yukio Nagahama, Masaki Takasu, Teruaki Tozaki

{"title":"Evaluation of genetic diversity using 31 microsatellites in Miyako horses.","authors":"Mioko Masuda, Akihiro Nikadori, Emi Nikadori, Naoya Uchida, Yoshimasa Takizawa, Mutsuki Ishimaru, Takumi Yamamoto, Reiko Yuasa, Haruo Kugai, Yukio Nagahama, Masaki Takasu, Teruaki Tozaki","doi":"10.1294/jes.34.1","DOIUrl":"https://doi.org/10.1294/jes.34.1","url":null,"abstract":"The Miyako horse is a native Japanese horse breed. As with other native Japanese horses, the number of Miyako horses decreased due to mechanization and motorization, which reduced their roles, with just 14 in 1980. Although their population had increased to 55 horses by 2021, a further increase in their numbers is required to avoid extinction. Recently, their breeding has involved natural mating during group grazing; therefore, pedigree management has been difficult, and individual identification has been inconclusive. With the aim of formulating an effective breeding plan, this study used microsatellites to confirm parent-offspring relationships and evaluate the genetic diversity over time. First, the combination of microsatellite genotypes identified misunderstood parent-offspring relationships in 35.3% of the existing individuals, and a correct family tree was reconstructed. Next, the number of alleles and observed and expected values of heterozygosity were calculated separately for the populations during periods of 1998-2012 and 2013-2020. The values were 4.2, 0.705, and 0.653 and 3.9, 0.633, and 0.603, respectively, indicating that genetic diversity according to all indices decreased during period of 2013-2020. This was probably because of the bias of stallions in the 2013-2020 population. Errors in pedigree information in a small population such as Miyako horses could increase the risk of inbreeding, and confirmation of parent-offspring relationships using genotypes may be beneficial. Additionally, to maintain diversity in future breeding, it is important to avoid bias, particularly among stallions, and to ensure offspring of various individuals who are as distantly related to each other as possible.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ab/15/jes-34-001.PMC10122988.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9431791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Prevalence of serum and salivary virus-neutralizing antibodies against equine coronavirus in four riding stables in Japan. 日本4个马场马冠状病毒血清和唾液病毒中和抗体的流行

Journal of Equine Science Pub Date : 2023-03-01 DOI: 10.1294/jes.34.13

Hiroshi Bannai, Yoshinori Kambayashi, Minoru Ohta, Manabu Nemoto, Koji Tsujimura

引用次数: 0