Journal of Equine Science最新文献_第3页

3D imaging and body measurement of riding horses using four scanners simultaneously. 同时使用四台扫描仪对骑马进行三维成像和身体测量。

Journal of Equine Science Pub Date : 2024-03-01 Epub Date: 2024-03-19 DOI: 10.1294/jes.35.1

Akihiro Matsuura, Suzuka Torii, Yuki Ojima, Yoshio Kiku

{"title":"3D imaging and body measurement of riding horses using four scanners simultaneously.","authors":"Akihiro Matsuura, Suzuka Torii, Yuki Ojima, Yoshio Kiku","doi":"10.1294/jes.35.1","DOIUrl":"10.1294/jes.35.1","url":null,"abstract":"Although there have been advances in the technology for measuring horse body size with stereoscopic three-dimensional (3D) scanners, previously reported methods with a single scanner still face a significant challenge: the time necessary for scanning is too long for the horses to remain stationary. This study attempted to scan the horse simultaneously from four directions using four scanners in order to complete the scans in a short amount of time and then combine the images from the four scans on a computer into one whole image of each horse. This study also compared body measurements from the combined 3D images with those taken from conventional manual measurements. Nine riding horses were used to construct stereoscopic composite images, and the following 10 measurements were taken: height at the withers, back, and croup; chest depth; width of the chest (WCh), croup, and waist; girth circumference, cannon circumference (CaC), and body length. The same 10 measurements were taken by conventional manual methods. Relative errors ranged from -1.89% to 7.05%. The correlation coefficient between manual and 3D measurements was significant for all body measurements (P<0.01) except for WCh and CaC. A simple regression analysis of all body measurements revealed a strong correlation (P<0.001, R2=0.9994, root-mean-square error=1.612). Simultaneous scanning with four devices from four directions reduced the scanning time from 60 sec with one device to 15 sec. This made it possible to perform non-contact body measurements even on incompletely trained horses who could not remain stationary for long periods of time.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"35 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10955269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum amino acid profiles in clinically normal Noma horses. 临床正常的坏疽性口炎马的血清氨基酸谱。

Journal of Equine Science Pub Date : 2024-01-01 Epub Date: 2024-07-03 DOI: 10.1294/jes.35.29

Keiichi Hisaeda, Tetsushi Ono, Sho Kadekaru, Akihisa Hata, Takako Shimokawa Miyama, Kenji Kutara, Keisuke Sugimoto, Yasuharu Hiasa, Emi Ohzawa, Tetsuo Kunieda, Eri Iwata, Hitoshi Kitagawa

{"title":"Serum amino acid profiles in clinically normal Noma horses.","authors":"Keiichi Hisaeda, Tetsushi Ono, Sho Kadekaru, Akihisa Hata, Takako Shimokawa Miyama, Kenji Kutara, Keisuke Sugimoto, Yasuharu Hiasa, Emi Ohzawa, Tetsuo Kunieda, Eri Iwata, Hitoshi Kitagawa","doi":"10.1294/jes.35.29","DOIUrl":"10.1294/jes.35.29","url":null,"abstract":"Plasma or serum amino acids are used to evaluate nutritional status and metabolic disorders. In this study, we aimed to set reference values of serum amino acid concentrations in the Noma horse, a Japanese native horse. Thirty-one horses were classified into six age groups: neonatal foal (0-4 days), foal (0.5-1 years), youth (5 years), middle age (10 years), old (15 years), and extra-old (>20 years). Horses >5 years of age were analyzed together as the adult group. In the adult horses, there were no significant differences among the serum amino acid concentrations of each age group. The foal group had higher concentrations of alanine, aspartic acid, glutamic acid, α-aminoadipic acid, and 3-methyl-histidine than the adult group. The neonatal foal group had higher serum concentrations of phenylalanine, lysine, alanine, proline, aspartic acid, glutamic acid, β-alanine, and β-amino-iso-butyric acid and lower tryptophan concentrations and Fischer's ratios than the adult group. The neonatal foal group had higher β-amino-iso-butyric acid concentrations and lower tryptophan and 3-methyl-histidine concentrations than the foal group. Therefore, reference values might be set separately in neonatal foals, foals, and adult horses. The data for the serum amino acid concentrations can be used for health care through physiological and pathological evaluations in Noma horses.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"35 2","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11219156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetic/pharmacodynamic analysis of cephalothin after intramuscular administration in Thoroughbred horses. 头孢噻吩在纯血马肌肉注射后的药代动力学/药效学分析。

Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.111

Taisuke Kuroda, Yohei Minamijima, Hidekazu Niwa, Hiroshi Mita, Norihisa Tamura, Kentaro Fukuda, Minoru Ohta

引用次数: 0

Postmortem magnetic resonance imaging findings of tricuspid atresia with ventricular and atrial septal defects and subvalvular pulmonic stenosis in a Japanese native Noma horse. 一匹日本原产野马的三尖瓣闭锁伴心室和房间隔缺损以及瓣下肺动脉狭窄的尸检磁共振成像结果。

Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.121

Kenji Kutara, Sho Kadekaru, Keiichi Hisaeda, Keisuke Sugimoto, Tetsushi Ono, Yoichi Inoue, Shinichi Nakamura, Ryohei Yoshitake, Emi Ohzawa, Akira Goto, Eri Iwata, Kenichi Shibano, Yumi Une, Hitoshi Kitagawa

引用次数: 0

Effects of concentrate levels on intestinal fermentation and the microbial profile in Japanese draft horses. 精料水平对日本草食马肠道发酵和微生物概况的影响。

Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.101

Rintaro Yano, Tomoe Moriyama, Miho Fujimori, Takehiro Nishida, Masaaki Hanada, Naoki Fukuma

{"title":"Effects of concentrate levels on intestinal fermentation and the microbial profile in Japanese draft horses.","authors":"Rintaro Yano, Tomoe Moriyama, Miho Fujimori, Takehiro Nishida, Masaaki Hanada, Naoki Fukuma","doi":"10.1294/jes.34.101","DOIUrl":"10.1294/jes.34.101","url":null,"abstract":"In racehorses, feeding a high-concentrate diet could cause abnormal fermentation in the hindgut. This feeding management regime is not suitable for the nutritional physiology of horses. However, studies on the hindgut environment have yet to be reported in Japanese draft horses, so feeding management needs to be investigated in these horses. Therefore, the objective of this study was to investigate the effects of a high-concentrate diet on hindgut fermentation in Japanese draft horses. Feces were collected from 20 male Japanese draft horses managed by two stables with different feeding designs (65% weight ratio of concentrate feed, HC; 50% weight ratio of concentrate, MC), and fecal metabolic characteristics and the microbiome were analyzed. Higher lactate concentrations and lower fecal pH levels were observed in the HC group (P=0.0011, P=0.0192, respectively). Fecal microbiome analysis revealed a decrease in microbial diversity (P=0.0360) and an increase in the relative abundance of Streptococcus lutetiensis/equinus/infantarius (P=0.0011) in the HC group. On the other hand, fibrolytic bacteria in the MC group had similarities with Clostridium sacchalolyticum and Ruminococcus albus. This study revealed that overfeeding of concentrates induced abnormal fermentation in the hindgut of Japanese draft horses. This suggests that the establishment of a feeding design based on not only the chemical compositions of feeds but also microbial dynamics is needed.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 4","pages":"101-109"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10806360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139564924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of Japanese native horse breeds by genotyping variants that are associated with phenotypic traits. 通过对与表型特征相关的变体进行基因分型，确定日本本土马匹品种的遗传特征。

Journal of Equine Science Pub Date : 2023-12-01 Epub Date: 2024-01-18 DOI: 10.1294/jes.34.115

Ibuki Saito, Kotono Nakamura, Teruaki Tozaki, Kazuki Hano, Masaki Takasu

引用次数: 0

Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells. 白藜芦醇及其类似物对马间充质干/基质细胞细胞周期的影响。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.67

Norihisa Tamura, Neda Heidari, Richard G A Faragher, Roger K W Smith, Jayesh Dudhia

{"title":"Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells.","authors":"Norihisa Tamura, Neda Heidari, Richard G A Faragher, Roger K W Smith, Jayesh Dudhia","doi":"10.1294/jes.34.67","DOIUrl":"https://doi.org/10.1294/jes.34.67","url":null,"abstract":"Resveratrol (RSV; trans-3,5,4'-trihydroxystilbene) strongly activates sirtuin 1, and it and its analogue V29 enhance the proliferation of mesenchymal stem/stromal cells (MSCs).Although culture medium containing 5-azacytydine and RSV inhibits senescence of adipose tissue-derived MSCs isolated from horses with metabolic syndrome, few studies have reported the effects of RSV on equine bone marrow-derived MSCs (eBMMSCs) isolated from horses without metabolic syndrome. The aim of this study was to investigate the effects of RSV and V29 on the cell cycle of eBMMSCs. Following treatment with 5 µM RSV or 10 µM V29, the cell proliferation capacity of eBMMSCs derived from seven horses was evaluated by EdU (5-ethynyl-2'-deoxyuridine) and Ki-67 antibody assays. Brightfield images of cells and immunofluorescent images of EdU, Ki-67, and DAPI staining were recorded by fluorescence microscopy, and the number of cells positive for each was quantified and compared by Friedman's test at P<0.05. The growth fraction of eBMMSCs was significantly increased by RSV and V29 as measured by the EdU assay (control 28.1% ± 13.8%, V29 31.8% ± 14.6%, RSV 32.0% ± 10.8%; mean ± SD; P<0.05) but not as measured by the Ki-67 antibody assay (control 27.0% ± 11.2%, V29 27.4% ± 10.8%, RSV 27.7% ± 6.8%). RSV and V29 promoted progression of the cell cycle of eBMMSCs into the S phase and may be useful for eBMMSC expansion.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 3","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0d/8c/jes-34-067.PMC10534064.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Construction of an individual identification panel for horses using insertion and deletion markers. 使用插入和删除标记构建马的个体识别面板。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-08-09 DOI: 10.1294/jes.34.83

Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Shun-Ichi Nagata

{"title":"Construction of an individual identification panel for horses using insertion and deletion markers.","authors":"Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Shun-Ichi Nagata","doi":"10.1294/jes.34.83","DOIUrl":"https://doi.org/10.1294/jes.34.83","url":null,"abstract":"Individual identification and paternity testing are important for avoiding inbreeding in the management of small populations of wild and domestic animals. In horse racing industries, they are extremely important for identifying and registering individuals and doping control to ensure fair competition. In this study, we constructed an individual identification panel for horses by using insertion and deletion (INDEL) markers. The panel included 39 INDEL markers selected from a whole-genome INDEL database. Genotyping of 89 Thoroughbreds showed polymorphisms with minor allele frequencies (MAFs) of 0.180-0.489 in all markers. The total probability of exclusion for paternity testing, power of discrimination, and probability of identity were 0.9994271269, >0.9999999999, and 0.9999999987, respectively. The panel was applied to 13 trios (sires, dams, and foals), and no contradictions were observed in genetic inheritance among the trios. When this panel was applied to the trios (52 trios) containing false fathers, an average of 7.3 markers excluded parentage relationships. In addition, genomic DNA extracted from the urine of six horses was partially genotyped for 39 markers, and 6-28 markers were successfully genotyped. The newly constructed panel has two advantages: a low marker mutation rate compared with short tandem repeats and a genotyping procedure that is as simple as short tandem repeat typing compared with single nucleotide variant typing. This panel can be applied for individual identification, paternity determination, and urine-sample identification in Thoroughbred horses.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 3","pages":"83-92"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/3b/jes-34-083.PMC10534061.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41172379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements. 马绞痛的严重程度与氧化应激生物标志物、急性期蛋白质和某些微量元素的关系。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.73

Ali Hajimohammadi, Mohsen Ghane, Masoud Ghari Tehrani, Benyamin Paravar, Ahmadreza Mirzaei, SeyedAmin Razavi, Mohammad Nikzad

{"title":"Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements.","authors":"Ali Hajimohammadi, Mohsen Ghane, Masoud Ghari Tehrani, Benyamin Paravar, Ahmadreza Mirzaei, SeyedAmin Razavi, Mohammad Nikzad","doi":"10.1294/jes.34.73","DOIUrl":"10.1294/jes.34.73","url":null,"abstract":"Sixty-one horses were enrolled in this study and divided into 3 different groups according to their severity of colic (heart rate, oral mucous membrane color, and abdominal distention): a strangulating colic (SC) group (n=21), non-strangulating colic (NC) group (n=20), and control group (n=20) consisting of randomly selected normal horses without signs of colic. The serum concentrations of haptoglobin, tumor necrosis factor-α (TNFα), nitric oxide (NO), malondialdehyde (MDA), zinc, iron, and copper were evaluated in all horses. The average concentration of TNFα in the SC group was higher than that in the control group (P<0.001). The TNFα concentration was higher in the NC group compared with the control group (P<0.001). Furthermore, the average concentration of TNFα tended to be higher in the SC group compared with the NC group (P=0.052). The average concentration of haptoglobin in the SC group was higher than that in the control group (P<0.001). The average concentration of NO was higher in the SC group compared with the NC group. (P=0.016) The average concentration of MDA was higher in the SC group compared with the control group (P=0.042). Furthermore, the concentration of MDA was higher in the SC group compared with the NC group (P=0.048). TNFα in horses with signs of colic may be a reliable indicator of prognosis and the severity of clinical signs. The haptoglobin concentration may be a useful marker in cases where animals are referred to clinicians a few days after the onset of colic. The concentrations of MDA and NO should be interpreted with caution.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 3","pages":"73-81"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8e/50/jes-34-073.PMC10534060.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41152614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence of surgical site infection after internal fixation of the first phalangeal bone and the third metacarpal/metatarsal bone fractures in Thoroughbred racehorses. 纯种赛马第一指骨和第三掌骨/跖骨骨折内固定后手术部位感染的发生率。

Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-09-08 DOI: 10.1294/jes.34.61

Hiroshi Mita, Taisuke Kuroda, Hidekazu Niwa, Norihisa Tamura, Kentaro Fukuda, Minoru Ohta

{"title":"Incidence of surgical site infection after internal fixation of the first phalangeal bone and the third metacarpal/metatarsal bone fractures in Thoroughbred racehorses.","authors":"Hiroshi Mita, Taisuke Kuroda, Hidekazu Niwa, Norihisa Tamura, Kentaro Fukuda, Minoru Ohta","doi":"10.1294/jes.34.61","DOIUrl":"10.1294/jes.34.61","url":null,"abstract":"Surgical site infection (SSI) is one of the major complications of equine fracture surgery. The purpose of this study was to investigate the incidence of and risk factors for SSI after internal fixation of the first phalangeal bone (P1) and the third metacarpal/metatarsal bone (MC3/MT3) fractures in Thoroughbred racehorses. Between 2011 and 2020, 451 cases underwent surgery with screws or a locking compression plate (LCP) for sagittal fractures of P1 or condylar fractures of MC3/MT3. Overall, 2.9% (13/451) of the cases developed an SSI. The incidence was significantly higher in plate fixation (21.4%) than in screw fixation (2.3%). There was no significant association with other variables, such as sex, age, number of screws, experience of surgeon, or prophylactic antimicrobials. The median duration of hospitalization for screw fixation was 14 days without an SSI and 20 days with an SSI, and those for plate fixation were 26 and 25-88 days, respectively, indicating that the development of SSI prolongs the duration of hospitalization. On the other hand, there were no significant differences in discharge and race resumption rates between cases with and without an SSI. These data indicate that the incidence of SSI in this study was low and that it was higher following plate fixation than screw fixation.","PeriodicalId":35701,"journal":{"name":"Journal of Equine Science","volume":"34 3","pages":"61-66"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/91/02/jes-34-061.PMC10534062.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41118457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0