使用插入和删除标记构建马的个体识别面板。

Q3 Veterinary
Journal of Equine Science Pub Date : 2023-09-01 Epub Date: 2023-08-09 DOI:10.1294/jes.34.83
Teruaki Tozaki, Aoi Ohnuma, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-Ichi Hirota, Shun-Ichi Nagata
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引用次数: 0

摘要

个体鉴定和亲子鉴定对于避免野生动物和家畜小种群管理中的近亲繁殖非常重要。在赛马行业,它们对于识别和登记个人以及兴奋剂控制以确保公平竞争极为重要。在这项研究中,我们使用插入和缺失(INDEL)标记构建了一个马的个体识别小组。该小组包括从全基因组INDEL数据库中选择的39个INDEL标记。89只纯种犬的基因分型显示多态性,所有标记的次要等位基因频率(MAFs)为0.180-0.489。亲子鉴定的总排除概率、辨别能力和同一性概率分别为0.9994271269、>0.9999999999和0.9999999987。该小组应用于13个三胞胎(父系、母系和小马驹),在三胞胎之间的遗传上没有发现矛盾。当这个小组应用于包含假父亲的三人组(52个三人组)时,平均有7.3个标记排除了父母关系。此外,从6匹马尿液中提取的基因组DNA进行了39个标记的部分基因分型,6-28个标记成功进行了基因分型。新构建的小组有两个优点:与短串联重复序列相比,标记突变率低,与单核苷酸变体分型相比,基因分型程序与短串联反复序列分型一样简单。该面板可用于纯种马的个体鉴定、亲子鉴定和尿样鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Construction of an individual identification panel for horses using insertion and deletion markers.

Construction of an individual identification panel for horses using insertion and deletion markers.

Individual identification and paternity testing are important for avoiding inbreeding in the management of small populations of wild and domestic animals. In horse racing industries, they are extremely important for identifying and registering individuals and doping control to ensure fair competition. In this study, we constructed an individual identification panel for horses by using insertion and deletion (INDEL) markers. The panel included 39 INDEL markers selected from a whole-genome INDEL database. Genotyping of 89 Thoroughbreds showed polymorphisms with minor allele frequencies (MAFs) of 0.180-0.489 in all markers. The total probability of exclusion for paternity testing, power of discrimination, and probability of identity were 0.9994271269, >0.9999999999, and 0.9999999987, respectively. The panel was applied to 13 trios (sires, dams, and foals), and no contradictions were observed in genetic inheritance among the trios. When this panel was applied to the trios (52 trios) containing false fathers, an average of 7.3 markers excluded parentage relationships. In addition, genomic DNA extracted from the urine of six horses was partially genotyped for 39 markers, and 6-28 markers were successfully genotyped. The newly constructed panel has two advantages: a low marker mutation rate compared with short tandem repeats and a genotyping procedure that is as simple as short tandem repeat typing compared with single nucleotide variant typing. This panel can be applied for individual identification, paternity determination, and urine-sample identification in Thoroughbred horses.

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来源期刊
Journal of Equine Science
Journal of Equine Science Veterinary-Equine
CiteScore
1.60
自引率
0.00%
发文量
9
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